Longevity in space; Experiment on the life span of Paramecium cell clone in space

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.     

Enzyme activities and membrane lipids in Artemia cysts after a long duration space flight

In the Free Flyer Biostack Experiment (L.D.E.F. mission) investigations have shown that biological objects in a resting state can survive more than 5.5 years of exposure to the space factors in particular microgravity and cosmic rays. We have measured enzyme activities involved in metabolic pathways of sugar and lipid degradation and determined phospholipid composition. Pyruvate kinase and glucose-6-phosphate dehydrogenase activities in space-exposed cysts were higher than in earth controls after 1 hour incubation. In controls, total phospholipids remained unchanged, on the contrary they increased significantly in space-exposed cysts. The rate of metabolism of various phospholipid components was unchanged in controls allowing the development while the level of most of them decreased in space-exposed cysts except for phosphatidylcholine. Enzyme activities (acetylhydrolase, phospholipase A₂ and lyso phospholipase) involved in phospholipid degradation increased; however, activities were much higher in space-exposed cysts. In conclusion, the long duration space flight resulted in an increase of the metabolic activity correlated with a faster development within the first 20 hours of post flight incubation.     

Daily variation characteristics at polar geomagnetic observatories

This paper is based on the statistical analysis of the diurnal variation as observed at six polar geomagnetic observatories, three in the Northern and three in the Southern hemisphere. Data are for 2006, a year of low geomagnetic activity. We compared the Italian observatory Mario Zucchelli Station (TNB; corrected geomagnetic latitude: 80.0°S), the French–Italian observatory Dome C (DMC; 88.9°S), the French observatory Dumont D’Urville (DRV; 80.4°S) and the three Canadian observatories, Resolute Bay (RES; 83.0°N), Cambridge Bay (CBB; 77.0°N) and Alert (ALE, 87.2°N). The aim of this work was to highlight analogies and differences in daily variation as observed at the different observatories during low geomagnetic activity year, also considering Interplanetary Magnetic Field conditions and geomagnetic indices.     

Optical and electron-microscopic studies of the Funaria hygrometrica protonema after cultivation for 96 days in space

$\text{Funaria hygrometrica}$ protonema cells grown in the “IFS-2” (Inoculating fixing system) for 96 days on board the Salyut 6 — Soyuz 32 orbital scientific station were examined by light and electron-microscopy. Investigation of experimental and control cells of the moss protonema showed common features as well as distinctions in their structure. Protonema cells of $\text{Funaria hygrometrica}$ both differentiate and undergo photosynthesis during space flight. Changes in cell shape, decreased cell size, a reduction in the volume of starch granules, and altered chloroplast structure were observed.     

Survey of the vestibulum, and behavior of xenopus laevis larvae developed during a 7-days space flight

Aquatic animals have almost no body weight related proprioception for spatial orientation. larvae, like fish, maintain their attitude in water by continuous correction with their fin(s). For these reasons a special performance of the equilibrium system compared to terrestrial animals is necessary. Evidently fish therefore have more compact (dense) otoliths; larvae have less dense otolith (membranes) similar to land vertebrates; but their sacculus-otoliths are vertically positioned, which also may lead to a higher g-sensitivity.

For plausibility reasons gravity should influence the embryonic development of gravity receptors. Yet, evaluations of photographs taken from the surface of cut deep-frozen objects by incident light show no aberration of the shape of the whole vestibulum and of the shape, density, size and position of the otolith membrane in larvae developed under near-zero g (NEXPA-BW-STATEX in D1-Mission).

The further evaluation of the “weightless-larvae” revealed a probably not yet described statolith-like formation in the dorsal wall of the vestibulum. In the weightless larvae this formation outnumbers, also qualitatively, strongly the 1-g controls.

An extra result is the lack of striking effects of cosmic radiation on the embryonic development of the flown eggs.

The swimming behavior of the larvae which was observed about one hour after landing of the Space Shuttle showed a typical anomaly (loop swimming), which is known from larvae developed on the clinostat or from fish flown aboard Apollo capsules.     

The X-ray evolution of galaxies: implications for future X-ray observatories

The X-ray evolution of the luminosity of normal galaxies is primarily driven by the evolution of their X-ray binary populations. The imprints left by a cosmological evolution of the star formation rate (SFR) will cause the average X-ray luminosity of galaxies to appear higher in the redshift range 1–3. As reported by White and Ghosh [ApJ, 504 (1998) L31] the profile of X-ray luminosity with redshift can both serve as a diagnostic probe of the SFR profile and constrain evolutionary models for X-ray binaries. In order to observe the high redshift (z>3) universe in the X-ray band, it is necessary to avoid confusion from foreground field galaxies. We report on the predictions of these models of the X-ray flux expected from galaxies and the implications for the telescope parameters of future deep universe X-ray observatories.     

Symmetry and asymmetry of ionospheric weather at magnetic conjugate points for two midlatitude observatories

Variations of the ionospheric weather W-index for two midlatitude observatories, namely, Grahamstown and Hermanus, and their conjugate counterpart locations in Africa are studied for a period from October 2010 to December 2011. The observatories are located in the longitude sector, which has consistent magnetic equator and geographic equator so that geomagnetic latitudes of the line of force are very close to the corresponding geographic latitudes providing opportunity to ignore the impact of the difference of the gravitational field and the geomagnetic field at the conjugate points on the ionosphere structure and dynamics. The ionosondes of Grahamstown and Hermanus provide data of the critical frequency (foF2), and Global Ionospheric Maps (GIM) provide the total electron content (TECgps) along the magnetic field line up to the conjugate point in the opposite hemisphere. The global model of the ionosphere, International Reference Ionosphere, extended to the plasmasphere altitude of 20,200 km (IRI-Plas) is used to deliver the F2 layer peak parameters from TECgps at the magnetic conjugate area. The evidence is obtained that the electron gas heated by day and cooled by night at the summer hemisphere as compared with the opposite features in the conjugate winter hemisphere testifies on a reversal of plasma fluxes along the magnetic field line by the solar terminator. The ionospheric weather W-index is derived from NmF2 (related with foF2) and TECgps data. It is found that symmetry of W-index behavior in the magnetic conjugate hemispheres is dominant for the equinoxes when plasma movement along the magnetic line of force is imposed on symmetrical background electron density and electron content. Asymmetry of the ionospheric storm effects is observed for solstices when the plasma diffuse down more slowly into the colder winter hemisphere than into the warmer summer hemisphere inducing either plasma increase (positive phase) or decrease (negative phase of W-index) in the ionospheric and plasmaspheric plasma density.     

Rosat observations of single pulsars

Recent Rosat results on the X-ray emission of single neutron stars are reviewed and compared to the the existing information.     

Capacity building in space law and space policy

A high level of expertise in space policy and law is required to initiate and keep pace with the expansion of space activities, including those undertaken by the private space industry. Space activities generally refer to those undertakings that are carried out with the use of several technologies for the exploration and utilization of outer space often for scientific, military, economic and social proposes within international and national policy and regulatory frameworks. Space policies and legal regimes determine the scope, nature, pace, possibility and development of space undertakings. Therefore, appropriate space policies and regulatory regimes, both at the international and national levels, are indispensable for the initiation, operation and enhancement of space activities. There are various regulatory models and approaches for regulating space activities, and for building capacity in space law and space policy. It is imperative to have the appropriate human resources and capabilities in the development and implementation of space policies and space legal regimes. This crucial requirement has been well recognized in the more advanced space-faring nations, but not to the same level in other space-faring or space-aspiring nations.     

Probing the galactic halo with ROSAT

We discuss the current status of ROSAT shadowing observations designed to search for emission from million degree gas in the halo of the Milky Way galaxy. Preliminary results indicate that million degree halo gas is observed in the 1/4 keV band in some directions, most notably toward the Draco cloud at (ℓ, b) = (92°, +38°), but that the halo emission is patchy and highly anisotropic. Our current understanding of this halo emission is based on a small handful of observations which have been analyzed to date. Many more observations are currently being analyzed or are scheduled for observation within the next year, and we expect our understanding of this component of the galactic halo to improve dramatically in the near future.     

Revealing the hot intergalactic medium with ROSAT

The X-ray background intensity around galaxies and rich clusters of galaxies is investigated in three energy bands using the ROSAT All-Sky Survey maps. It is found that an amplitude of the XRB enhancements surrounding the Abell clusters and high density areas in the Lick galaxy counts depends on photon energy. Excess flux generated in the surrounding of the galaxy concentrations is consistent with the thermal emission by hot gas postulated by hydrodynamic simulations.     

Evolution of coronal mass ejection/shock system in interplanetary space

The evolution of coronal mass ejection/shock system is investigated by numerically solving the usual set of two-dimensional single-fluid polytropic magnetohydrodynamic equations from 1 R_s to 1 AU in the meridian plane. The simulation result reveals that the coronal mass ejection/shock system formed near the sun evolves into the magnetic cloud/shock system near the earth’s orbit through the following three phases: the initial formation, the dominant latitudinal expansion and the similar expansion.     

从时空里归来

1979年,我被外派到巴拿马籍的荒原日出号工作,同时被外派的还有1名木匠、4名水手和1名服务员.船队中还有来自意大利的高级船员、菲律宾和印度的技工等,是多国船员组成的船队.     

A statistical analysis of low frequency geomagnetic field pulsations at two Antarctic geomagnetic observatories in the polar cap region

The aim of this study is to investigate the characteristics of low frequency (∼0.5–5 mHz) geomagnetic field fluctuations as recorded at two Antarctic stations within the polar cap: the Italian observatory Mario Zucchelli Station (TNB) and the French–Italian observatory Dome C (DMC) in order to investigate the spatial extension and propagation characteristics of the phenomena observed at very high latitude. The stations have approximately the same geographic latitude, but a very different corrected geomagnetic latitude, being DMC close to the geomagnetic pole and TNB closer to the auroral oval.     

Evaluation of gravity-dependent membrane potential shift in Paramecium

It is still debated whether or not gravity can stimulate unicellular organisms. This question may be settled by revealing changes in the membrane potential in a manner depending on the gravitational forces imposed on the cell. We estimated the gravity-dependent membrane potential shift to be about 1 mV G⁻¹ for Paramecium showing gravikinesis at 1–5 G, on the basis of measurements of gravity-induced changes in active propulsion and those of propulsive velocity in solutions, in which the membrane potential has been measured electrophysiologically. The shift in membrane potential to this extent may occur from mechanoreceptive changes in K⁺ or Ca²⁺ conductance by about 1% and might be at the limit of electrophysiological measurement using membrane potential-sensitive dyes. Our measurements of propulsive velocity vs membrane potential also suggested that the reported propulsive force of Paramecium measured in a solution of graded densities with the aid of a video centrifuge microscope at 350 G was 11 times as large as that for −29 mV, i.e., the resting membrane potential at [K⁺]_o = 1 mM and [Ca²⁺]_o = 1 mM, and, by extrapolation, that Paramecium was hyperpolarized to −60 mV by gravity stimulation of 100- G equivalent, the value corrected by considering the reduction of density difference between the interior and exterior of the cell in the graded density solution. The estimated shift of the membrane potential from −29 mV to −60 mV by 100- G equivalent stimulation, i.e., 0.3 mV G⁻¹, could reach the magnitude entirely feasible to be measured more directly.     

ROSAT observations of the Chamaeleon star forming cloud

Two soft X-ray images of the Chamaeleon I star forming cloud obtained with the ROSAT Position Sensitive Proportional Counter are presented. Seventy reliable, and perhaps 19 additional, X-ray sources are found. Up to Ninety percent of these sources are certainly or probably identified with T Tauri stars formed in the cloud. Twenty to 35 are probably previously unrecognized ‘weak’ T Tauri (WTT) stars. T Tauri X-ray luminosities range from log , or 10² – 10⁴ times solar levels, with mean in the 0.2–2.5 keV band. The X-ray luminosities of well-studied Chamaeleon cloud members are correlated with a complex of four stellar properties: effective temperature, mass, radius and bolometric luminosity. The spatial distribution, H-R diagram locations of the stars indicate WTT and CTT are coeval. The total premain sequence population of the cloud is likely to be > 100 stars, with WTT stars outnumbering ‘classical’ T Tauri (CTT) stars by 2:1.     

Laboratory experiments and space phenomena

Two types of convection were observed in the laboratory model of the magnetosphere: viscous convection and convection due to field lines common to both the magnetosphere and artificial solar wind. With a southward field component in the solar wind, convection from the Sun is observed in the polar cap, while with a large northward component, convection is directed toward the Sun. Merging of the field lines occurs in the cleft. With the southward component, a visor appears in front of the magnetosphere boundary. The decay of the visor into small magnetic structure is observed. The formation of an induced magnetosphere with a magnetic tail is shown in the experiments of the simulated conditions near non-magnetic bodies with a plasma shell (Venus, comets). A combined induced-intrinsic magnetosphere also was investigated.