The features of radiation dose variations onboard ISS and Mir space station: comparative study.

The dynamics of the ISS-measured radiation dose variations since August 2000 is studied. Use is made of the data obtained with the R-16 instrument, which consists of two ionization chambers behind different shielding thicknesses. The doses recorded during solar energetic particle (SEP) events are compared with the data obtained also by R-16 on Mir space station. The SEP events in the solar maximum of the current cycle make a much smaller contribution to the radiation dose compared with the October 1989 event recorded on Mir space station. In the latter event, the proton intensity was peaking during a strong magnetic storm. The storm-time effect of solar proton geomagnetic cutoff decreases on dose variations is estimated. The dose variations on Mir space stations due to formation of a new radiation belt of high-energy protons and electrons during a sudden commencement of March 24, 1991 storm are also studied. It was for the first time throughout the ISS and Mir dose measurement period that the counting rates recorded by both R-16 channels on ISS in 2001-2002 were nearly the same during some time intervals. This effect may arise from the decreases of relativistic electron fluxes in the outer radiation belt.     

Peculiarities of the solar proton events of 19 October 1989 and 23 March 1991 according to the measurements onboard the Mir space station.

Flux and dose rate dynamics of solar cosmic rays were measured by the Lyulin dosimeter during the events 19 October 1989 and 23 March 1991. The maximum dose rate registered was 0.4, 0.12 and 0.01 cGy/hour, respectively. Based on the latitude distribution of particle flux a power law form for the energy spectra of solar protons in the anisotropic phase of the events on 19 October 1989 and 23 March 1991 was determined. It was obtained that after the development of geomagnetic storm protons with energies more than 1 GeV were registered.     

Austrian dose measurements onboard space station MIR and the International Space Station--overview and comparison.

The Atominstitute of the Austrian Universities has conducted various space research missions in the last 12 years in cooperation with the Institute for Biomedical Problems in Moscow. They dealt with the exact determination of the radiation hazards for cosmonauts and the development of precise measurement devices. Special emphasis will be laid on the last experiment on space station MIR the goal of which was the determination of the depth distribution of absorbed dose and dose equivalent in a water filled Phantom. The first results from dose measurements onboard the International Space Station (ISS) will also be discussed. The spherical Phantom with a diameter of 35 cm was developed at the Institute for Biomedical Problems and had 4 channels where dosimeters can be exposed in different depths. The exposure period covered the timeframe from May 1997 to February 1999. Thermoluminescent dosimeters (TLDs) were exposed inside the Phantom, either parallel or perpendicular to the hull of the spacecraft. For the evaluation of the linear energy transfer (LET), the high temperature ratio (HTR) method was applied. Based on this method a mean quality factor and, subsequently, the dose equivalent is calculated according to the Q(LET infinity) relationship proposed in ICRP 26. An increased contribution of neutrons could be detected inside the Phantom. However the total dose equivalent did not increase over the depth of the Phantom. As the first Austrian measurements on the ISS dosimeter packages were exposed for 248 days, starting in February 2001 at six different locations onboard the ISS. The Austrian dosimeter sets for this first exposure on the ISS contained five different kinds of passive thermoluminescent dosimeters. First results showed a position dependent absorbed dose rate at the ISS.     

Study of the radiation environment on MIR space station with SILEYE-2 experiment.

In this work we present preliminary results of nuclear composition measurements on board space station MIR obtained with SILEYE-2 particle telescope. SILEYE-2 was placed on MIR in 1997 and has been working since then. It consists of an array of 6 active silicon strip detectors which allow nuclear and energetic identification of cosmic rays in the energy range between approximately 30 and 200 MeV/n. The device is attached to an helmet and connected to an eye mask which shields the cosmonaut eyes from light and allow studies of the Light Flashes (LF) phenomenon. In addition to the study of the causes of LF, the device is used to perform real time long term radiation environment monitoring inside the MIR, performing measurements in solar quiet and active days.     

Recent space shuttle observations of the South Atlantic Anomaly and the radiation belt models.

Active instruments consisting of a tissue equivalent proportional counter (TEPC) and a proton and heavy ion detector (PHIDE) have been carried on a number of Space Shuttle flights. These instruments have allowed us to map out parts of the South Atlantic Particle Anomaly (SAA) and to compare some of its features with predictions of the AP-8 energetic proton flux models. We have observed that consistent with the generally observed westward drift of the surface features of the terrestrial magnetic field the SAA has moved west by about 6.9 degrees longitude between the epoch year 1970 of the AP-8 solar maximum model and the Space Shuttle observations made twenty years later. However, calculations indicate that except for relatively brief periods following very large magnetic storms the SAA seems to occupy the same position in L-space as in 1970. After the great storm of 24 March 1991 reconfiguration of the inner radiation belt and/or proton injection into the inner belt, a second energetic proton belt was observed to form at L approximately = 2. As confirmed by a subsequent flight observations, this belt was shown to persist at least for six months. Our measurements also indicate an upward shift in the L location of the primary belt from L = 1.4 to L = 1.5. In addition we confirm through direct real time observations the existence and the approximate magnitude of the East-West effect.     

Improvements in and actual performance of the Plant Experiment Unit onboard Kibo,the Japanese experiment module on the international space station

In 2004, Japan Aerospace Exploration Agency developed the engineered model of the Plant Experiment Unit and the Cell Biology Experiment Facility. The Plant Experiment Unit was designed to be installed in the Cell Biology Experiment Facility and to support the seed-to-seed life cycle experiment of Arabidopsis plants in space in the project named Space Seed. Ground-based experiments to test the Plant Experiment Unit showed that the unit needed further improvement of a system to control the water content of a seedbed using an infrared moisture analyzer and that it was difficult to keep the relative humidity inside the Plant Experiment Unit between 70 and 80% because the Cell Biology Experiment Facility had neither a ventilation system nor a dehumidifying system. Therefore, excess moisture inside the Cell Biology Experiment Facility was removed with desiccant bags containing calcium chloride. Eight flight models of the Plant Experiment Unit in which dry Arabidopsis seeds were fixed to the seedbed with gum arabic were launched to the International Space Station in the space shuttle STS-128 (17A) on August 28, 2009. Plant Experiment Unit were installed in the Cell Biology Experiment Facility with desiccant boxes, and then the Space Seed experiment was started in the Japanese Experiment Module, named Kibo, which was part of the International Space Station, on September 10, 2009 by watering the seedbed and terminated 2 months later on November 11, 2009. On April 19, 2010, the Arabidopsis plants harvested in Kibo were retrieved and brought back to Earth by the space shuttle mission STS-131 (19A). The present paper describes the Space Seed experiment with particular reference to the development of the Plant Experiment Unit and its actual performance in Kibo onboard the International Space Station. Downlinked images from Kibo showed that the seeds had started germinating 3 days after the initial watering. The plants continued growing, producing rosette leaves, inflorescence stems, flowers, and fruits in the Plant Experiment Unit. In addition, the senescence of rosette leaves was found to be delayed in microgravity.     

Distribution of energetic particles and secondary radiation according to orbital station “MIR” data obtained in 1991

A set of instruments for measuring energetic particle fluxes, containing two neutron detectors under different plexiglas shielding thicknesses, a scintillation detector, measuring energy release >0. I MeV and 0.5 MeV and a Geiger counter were launched onboard OS ‘MIR’. The latitude dependencies of the cosmic ray measurements were obtained and studied. The distributions of primary particle fluxes (protons and electrons) as well as secondary particle fluxes (bremsstrahlung gamma-rays and neutrons) produced in interactions of radiation belt particles with the station materials were obtained. The electron belt, generated during the storm of March 24 1991, is studied.     

Aquatic animal research in space station and its issues--focus on support technology on nitrate toxicity.

We studied the effects of accumulated nitrate in water on the spawning, hatching and development of medaka using a simple nitrifying filter and a combined filter having both nitrifying and denitrifying capabilities. A nitrate concentration of 100 mg NO3(-)-N/L was clearly of lethal toxicity to fish when they were exposed to nitrate in both adult and the growing phases. A nitrate concentration of 75 mg NO3(-)-N/L reduced the fertilization rate, delayed the hatching time and reduced the hatching rate of the eggs laid by adults and decreased the growth rate of juveniles. In addition, nitrate accumulations as low as 50 mg NO3(-)-N/L remarkably retarded spawning and lowered the number of eggs laid by fish exposed in the juvenile phase. The effects on the reproduction system may be initiated by a low concentration, approximately 30 mg NO3(-)-N/L.     

Cataract analysis and the assessment of radiation risk in space.

Radiation cataract, a non-stochastic effect on the lens, is readily amenable to non-invasive analysis. Thus, it provides the means to assess radiation risk in space and for long-term monitoring of those who frequent that environment. The importance of such evaluations are underscored by the uncertainties associated with the assignment of quality factors for the effects of heavy charged particles constituting cosmic and solar radiation. Experimental studies were conducted using albino rats to evaluate the cataractogenic potential of 570 MeV/amu Argon ions administered as both single and protracted doses. The cataract studies and investigations of quantitative cytopathological changes associated with them indicate that as the dose of heavy particles decreases, the relative biological effectiveness, compared to X rays, increases. Fractionating the exposures not only failed to reduce the cataractogenic effect but caused a dose-dependent enhancement in the time of onset of opacification. Cytopathologically, the damage caused by heavy particles, when compared to low-LET radiation was found to be quantitatively dissimilar but qualitatively identical. In addition, damage which might be consistent with microlesions was not evident. The data indicates that as regards the cataractogenic potential of heavy particles at low doses an assignment of a Quality Factor (QF) of at least 40 may be in order.     

Cellular changes in microgravity and the design of space radiation experiments.

Cell metabolism, secretion and cell-cell interactions can be altered during space flight. Early radiobiology experiments have demonstrated synergistic effects of radiation and microgravity as indicated by increased mutagenesis, increased chromosome aberrations, inhibited development, and retarded growth. Microgravity-induced changes in immune cell functions include reduced blastogenesis and cell-mediated, delayed-type hypersensitivity responses, increased cytokine secretions, but inhibited cytotoxic effects and macrophage differentiation. These effects are important because of the high radiosensitivity of immune cells. It is difficult to compare ground studies with space radiation biology experiments because of the complexity of the space radiation environment, types of radiation damage and repair mechanisms. Altered intracellular functions and molecular mechanisms must be considered in the design and interpretation of space radiation experiments. Critical steps in radiocarcinogenesis could be affected. New cell systems and hardware are needed to determine the biological effectiveness of the low dose rate, isotropic, multispectral space radiation and the potential usefulness of radioprotectants during space flight.     

Feasibility study of LEO,GEO and Molniya orbit based satellite solar power station for some identified sites in India

The analysis of satellite solar power station (SSPS) is carried out for some specified locations (Delhi, Mumbai, Kolkata and Bengaluru) in India and consequently the performance of the system is evaluated for geostationary earth orbit (GEO) based SSPS, low earth orbit (LEO) based SSPS and Molniya (quasi geostationary) orbit based SSPS for sites located at different latitudes. The analysis of power; received energy over a year and weight of the rectenna array for the same beam intensity showed varied results for Molniya orbit based SSPS, LEO based SSPS and GEO based SSPS. The power delivered by the LEO SSPS was highest which indicated that this SSPS may be efficient for the short term power requirement. However, it is observed from the results of the energy received over a year that the GEO based system is suitable for base load power plant as it is capable of delivering constant energy through out a year. Further, the weight of the rectenna and hence the space required for ground station for same power output is smallest for Molniya orbit based system for a range of rectenna array radius considered. It is thus envisaged that the Molniya orbit based system would be a better choice for commercial use of SSPS. These findings may help for judicious selection of satellite orbit and ground station location for placing the satellite for SSPS for various applications.     

Definition of the lower hybrid frequency by active wave methods on the intercosmos-24 satellite and comparison of these data with mass-spectrometric measurements

The lower hybrid resonance (LHR) frequency estimated from the mass-spectrometer data is compared with measurements using spectrograms of VLF noise excited by both natural sources and through active wave stimulation of the surrounding plasma. The measurements are from the Intercosmos 24 satellite taken over a 2 year period from 1989–1991.     

Effect of the space environment on the induction of DNA-repair related proteins and recovery from radiation damage.

Recovery of bacterial cells from radiation damage and the effects of microgravity were examined in an STS-79 Shuttle/Mir Mission-4 experiment using the extremely radioresistant bacterium Deinococcus radiodurans. The cells were irradiated with gamma rays before the space flight and incubated on board the Space-Shuttle. The survival of the wild type cells incubated in space increased compared with the ground controls, suggesting that the recovery of this bacterium from radiation damage was enhanced under microgravity. No difference was observed for the survival of radiosensitive mutant rec30 cells whether incubated in space or on the ground. The amount of DNA-repair related RecA protein induced under microgravity was similar to those of ground controls, however, induction of PprA protein, the product of a newly found gene related to the DNA repair mechanism of D. radiodurans, was enhanced under microgravity compared with ground controls.     

Investigation of dose and flux dynamics in the Liulin-5 dosimeter of the tissue-equivalent phantom onboard the Russian segment of the International Space Station.

Described is the Liulin-5 active dosimetric telescope designed for measurement of the space radiation dose depth-distribution in a human phantom on the Russian Segment of the International Space Station (ISS). The Liulin-5 experiment is a part of the international project MATROSHKA-R on ISS. The MATROSHKA-R project is aimed to study the depth-dose distribution at the sites of critical organs of the human body, using models of human body-anthropomorphic and spherical tissue-equivalent phantoms. The aim of Liulin-5 experiment is a long term (4-5 years) investigation of the radiation environment dynamics inside the spherical tissue-equivalent phantom, mounted in different compartments. Energy deposition spectra, linear energy transfer spectra, and flux and dose rates for charged particles will be measured simultaneously with near real time resolution at different depths of the phantom by means of three silicon detectors. Data obtained together with data from other active and passive dosimeters will be used to estimate the radiation risk to the crewmembers, which verify the models of radiation environment in low Earth orbit. Presented are the test results of the prototype unit. Liulin-5 will be flown on the ISS in the year 2003.     

Preliminary results of space experiment: Implications for the effects of space radiation and microgravity on survival and mutation induction in human cells

In view of the concern for the health of astronauts that may one day journey to Mars or the Moon, we investigated the effect that space radiation and microgravity might have on DNA damage and repair. We sent frozen human lymphoblastoid TK6 cells to the International Space Station where they were maintained under frozen conditions during a 134-day mission (14 November 2008 to 28 March 2009) except for an incubation period of 8 days under 1G or μG conditions in a CO₂ incubator. The incubation period started after 100 days during which the cells had been exposed to 54 mSv of space radiation. The incubated cells were then refrozen, returned to Earth, and compared to ground control samples for the determination of the influence of microgravity on cell survival and mutation induction. The results for both varied from experiment to experiment, yielding a large SD, but the μG sample results differed significantly from the 1G sample results for each of 2 experiments, with the mean ratio of μG to 1G being 0.55 for the concentration of viable cells and 0.59 for the fraction of thymidine kinase deficient (TK⁻) mutants. Among the mutants, non-loss of zygosity events (point mutations) were less frequent (31%) after μG incubation than after 1G incubation, which might be explained by the influence of μG on cellular metabolic or physiological function. Additional experiments are needed to clarify the effect of μG interferes on DNA repair.     

Estimation of risk based on multiple events in radiation carcinogenesis of rat skin.

In the multistage theory of carcinogenesis, cells progress to cancer through a series of discrete, irreversible, heritable genetic alterations or mutations. However data on radiation-induced cancer incidence in rat skin suggests that some part of an intermediate repairable alteration may occur. Data are presented on cancer induction in rat skin exposed to the following radiations: 1. an electron beam (LET=0.34 keV/um, 2. a neon ion beam (LET=25 keV/um and 3. an argon ion beam (LET=125 keV/um. The latter 2 beams were generated by the Bevalac at the Lawrence Berkeley Laboratory, Berkeley, CA. About 6.0 cm2 of skin was irradiated per rat. The rats were observed every 6 weeks for at least 78 weeks and tumors were scored at first occurrence. Several histological types of cancer, including squamous and basal cell carcinomas, were induced. The cancer yield versus radiation dose was fitted by the quadratic equation (Y(D)=CLD+BD2), and the parameters C and B were estimated for each type of radiation. Analysis of the DNA from the electron-induced carcinomas indicated that K-ras and/or c-myc oncogenes were activated in all tumors tested, although only a small proportion of neon-induced tumors showed similar activation. In situ hybridization indicated that the cancers contain subpopulations of cells with differing amounts of c-myc and H-ras amplification. The results are consistent with the idea that ionizing radiation produces carcinogenically relevant lesions via 2 repairable events at low LET and via a non-repairable, linked event pathway at high LET; either pathway may advance the cell by 1 stage in the multistage model. The model, if validated, permits the direct calculation of cancer risk in rat skin in a way that can be subjected to experimental testing.     

Initial approach to comparative studies on the evolutionary potentials of space radiation effects in a plant system.

The role of cosmic ionizing radiation, including heavy ions (HZE-particles) in the induction of mutations at the molecule-, chromosome-, genome- and cell-level is discussed on the basis of different DNA organization in a pro- and eukaryotically compartmented plant system (Arabidopsis thaliana (L.) Heynh.). Data recently obtained on the biological effects of ionizing radiation make it timely to discuss comparatively the evolutionary potentials of space radiation effects in the pro- and eukaryotic genomes (plasmon, plastidom, chondriom, and nucleom) during long duration exposure on space flights.     

A model of radiation conditions during spacecraft flights in the interplanetary space and in the Earth's magnetosphere.

Based on the available measurement data, simulations of radiation conditions during spacecraft flights in the interplanetary space and in the Earth's and Jupiter's radiation belts has been carried out. The > or = 10 MeV and > or = 30 MeV solar flare proton fluence forecast has been proposed for Cycle 22. Radiation conditions due to both magnetospheric electrons and protons and to solar flare protons, magnetic rigidity cutoff being taken into account, have been evaluated on spacecraft trajectories in the Earth's and Jupiter's magnetospheres.     

Effects of long duration space flight on rice seed (or embryo) radiation sensitivity and element microlocalizations.

In long duration space experiments Rice caryopses and embryos, which are able to remain alive 10 years (or more) and tolerate extreme physical conditions (temperature, few water content) during irradiation and post-irradiation storage, were used (8, 40, 201 and 457 days on board of Salyut 7, 2107 days on LDEF). In certain experiments (Salyut 7), samples were irradiated either before or after the flight. Effects of the flight and radiosensitivity were observed in Rice seedlings cultivated in in vitro conditions. Statistical results indicate an increase in radiosensitivity when irradiations occur before the flight. Microanalyses were made in different parts of one caryopsis and of one embryo, and the results compared with those of control samples. With caryopses and embryos of the same Rice varieties, but from LDEF, we made the same kinds of experiments to compare results.     

Solar-interplanetary circumstances associated with the major events in March and June 1991 and comparison with similar events of previous solar cycles.

The solar activity and geomagnetic storm events of March and June 1991 were associated with the appearance of an enhanced particle flux in the trapped radiation belts as discovered by the CRRES satellite observations and later measured by shuttle radiation detectors. The solar-interplanetary conditions associated with these events appear to be a major sequence of activity near the sun's central meridian generating powerful fast interplanetary shocks resulting in major perturbations to the magnetosphere. The solar-interplanetary events in 1991 are discussed and compared to similar activity in the past such as the events in February 1986, August 1972, July 1961, November 1960, and July 1959.