Equatorial M(3000)F2 estimation of F2-layer models peak heights during low solar activity

M(3000)F2 estimation of h_mF2 based on four different formulated models viz: (1) Shimazaki (1955) (2) Bradley and Dudeney (1973), (3) Dudeney (1974) and (4) Bilitza et al. (1979) at an equatorial station in West Africa during low solar activity period (1995) are used to validate its conformity with observed and International Reference Ionosphere (IRI) model. Local time analyses of data from fifteen (15) selected days during the January and July solstices and April and October equinoxes are used. The results obtained show that the M(3000)F2 estimation of h_mF2 from the ionosonde-measured values using the Ionospheric Prediction Service (IPS-42) sounder compared to the observed values which were deduced using an algorithm from scaled virtual heights of quiet day ionograms are highly correlated with Bilitza model. International Reference Ionosphere (IRI 2007) model for the equatorial region also agrees with the formulation developed by Bilitza et al. (1979) for the four different seasons of the year. h_mF2 is highest (425 km) in summer (June solstice) season and lowest (386 km) in autumn (September equinox) season with daytimes peaks occurring at 1100–1200 LT during the solstices and at 1000 LT during the equinoxes respectively. Also, the post-sunset peaks are highest (362 km) at the spring (March equinox) and lowest (308 km) at the summer (June solstice) both occurring between 1800 and 2000 LT.     

Effect of vertical plasma transport on ionospheric F2-region parameters at equatorial latitude

The variability of the F2-layer even during magnetically quiet times are fairly complex owing to the effects of plasma transport. The vertical E × B drift velocities (estimated from simplified electron density continuity equation) were used to investigate the seasonal effects of the vertical ion drifts on the bottomside daytime ionospheric parameters over an equatorial latitude in West Africa, Ibadan, Nigeria (Geographic: 7.4°N, 3.9°E, dip angle: 6°S) using 1 year of ionsonde data during International Geophysical Year (IGY) of 1958, that correspond to a period of high solar activity for quiet conditions. The variation patterns between the changes of the vertical ion drifts and the ionospheric F2-layer parameters, especially; f_oF₂ and h_mF₂ are seen remarkable. On the other hand, we observed strong anti-correlation between vertical drift velocities and h′F in all the seasons. We found no clear trend between N_mF₂ and h_mF₂ variations. The yearly average value of upward daytime drift at 300 km altitude was a little less than the generally reported magnitude of 20 ms⁻¹ for equatorial F-region in published literature, and the largest upward velocity was roughly 32 ms⁻¹. Our results indicate that vertical plasma drifts; ionospheric F2-layer peak height, and the critical frequency of F2-layer appear to be somewhat interconnected.     

Global comparison of the model results of GSM TIP with IRI for summer conditions

This paper presents the results of the numerical calculations thermosphere/ionosphere parameters which were executed with using of the Global Self-consistent Model of the Thermosphere, Ionosphere and Protonosphere (GSM TIP)and comparison of these results with empirically-based model IRI-2001. Model GSM TIP was developed in West Department of IZMIRAN and solves self-consistently the time-dependent, 3-D coupled equations of the momentum, energy and continuity for neutral particles (O₂, N₂, O), ions (O⁺, H⁺), molecular ions (M⁺) and electrons and largescale eletric field of the dynamo and magnetospheric origin in the range of height from 80 km to 15 Earth’s radii. The empirically derived IRI model describes the E and F regions of the ionosphere in terms of location, time, solar activity and season. Its output provides a global specification not only of N_e but also on the ion and electron temperatures and the ion composition. These two models represent a unique set of capabilities that reflect major differences in along with a substantial approaches of the first-principles model and global database model for the mapping ionosphere parameters. We focus on global distribution of the N_e, T_i, T_e and TEC for the one moment UT and fixed altitudes: 110 km, h_mF2, 300 km and 1000 km. The calculations were executed with using of GSM TIP and IRI models for August 1999, moderate solar activity and quiet geomagnetic conditions. Results present as the global differences between the IRI and GSM TIP models predictions. The discrepancies between model results are discussed.     

Variation in the ionospheric propagating factor M(3000)F2 at Ouagadougou,Burkina Faso

We use hourly monthly median values of propagation factor M(3000)F2 data observed at Ouagadougou Ionospheric Observatory (geographic12.4°N, 1.5°W; 5.9^o dip), Burkina Faso (West Africa) during the years Januar1987–December1988 (average F10.7 < 130 × 10⁻²² W/m²/Hz, representative of low solar flux conditions) and for January 1989–December1990 (average F10.7 ? 130 × 10⁻²² W/m²/Hz, representative of high solar epoch) for magnetically quiet conditions to describe local time, seasonal and solar cycle variations of equatorial ionospheric propagation factor M(3000)F2 in the African region. We show that that seasonal trend between solar maximum and solar minimum curves display simple patterns for all seasons and exhibits reasonable disparity with root mean square error (RMSE) of about 0.31, 0.29 and 0.26 for December solstice, June solstice and equinox, respectively. Variability Σ defined by the percentage ratio of the absolute standard deviation to the mean indicates significant dissimilarity for the two solar flux levels. Solar maximum day (10–14 LT) and night (22–02 LT) values show considerable variations than the solar minimum day and night values. We compare our observations with those of the IRI 2007 to validate the prediction capacity of the empirical model. We find that the IRI model tends to underestimate and overestimate the observed values of M(3000)F2, in particular, during June solstice season. There are large discrepancies, mainly during high solar flux equinox and December solstice between dawn and local midnight. On the other hand, IRI provides a slightly better predictions for M(3000)F2 between 0900 and 1500 LT during equinox low and high solar activity and equinox high sunspot number. Our data are of great importance in the area of short-wave telecommunication and ionospheric modeling.     

An alternative Shell inversion technique - Analysis and validation based on COSMIC and ionosonde data

Multi-channel Global Positioning System (GPS) carrier phase signals, received by the six low Earth orbiting (LEO) satellites from the Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC) program, were used to undertake active limb sounding of the Earth’s atmosphere and ionosphere via radio occultation. In the ionospheric radio occultation (IRO) data processing, the standard Shell inversion technique (SIT), transformed from the traditional Abel inversion technique (AIT), is widely used, and can retrieve good electron density profiles. In this paper, an alternative SIT method is proposed. The comparison between different inversion techniques will be discussed, taking advantage of the availability of COSMIC datasets. Moreover, the occultation results obtained from the SIT and alternative SIT at 500 km and 800 km, are compared with ionosonde measurements. The electron densities from the alternative SIT show excellent consistency to those from the SIT, with strong correlations over 0.996 and 0.999 at altitudes of 500 km and 800 km, respectively, and the peak electron densities (N_mF₂) from the alternative SIT are equivalent to the SIT, with 0.839 vs. 0.844, and 0.907 vs. 0.909 correlation coefficients when comparing to those by the ionosondes. These results show that: (1) the N_mF₂ and h_mF₂ retrieved from the SIT and alternative SIT are highly consistent, and in a good agreement with those measured by ionosondes, (2) no matter which inversion technique is used, the occultation results at the higher orbits (∼800 km) are better than those at the lower orbits (∼500 km).     

A different method to update monthly median hmF2 values

The height, h_mF2, and the electron density, N_mF2, of the F2 peak are key model parameters to characterize the actual state of the ionosphere. These parameters, or alternatively the propagation factor, M3000F2, and the critical frequency, f_oF2, of the F2 peak, which are related to h_mF2 and N_mF2, are used to anchor the electron density vertical profile computed with different models such as the International Reference Ionosphere ( Bilitza, 2002), as well as for radio propagation forecast purposes. Long time series of these parameters only exist in an inhomogeneous distribution of points over the surface of Earth, where dedicated instruments (typically ionosondes) have been working for many years. A commonly used procedure for representing median values of the aforementioned parameters all over the globe is the one recommended by the ITU-R ( ITU-R, 1997). This procedure, known as the Jones and Gallet mapping technique, was based on ionosondes measurements gathered from 1954 to 1958 by a global network of around 150 ionospheric stations (  and ). Even though several decades have passed since the development of that innovative work, only few efforts have been dedicated to establish a new mapping technique for computing h_mF2 and N_mF2 median values at global scale or to improve the old method using the increased observational database. Therefore, in this work three different procedures to describe the daily and global behavior of the height of the F2 peak are presented. All of them represent a different and simplified method to estimate h_mF2 and are based on different mathematical expressions. The advantages and disadvantages of these three techniques are analyzed, leading to the conclusion that the recommended procedure to represent h_mF2 is best characterized by a Spherical Harmonics expansion of degree and order equal to 15, since the differences between the h_mF2 values obtained with the Jones and Gallet technique and those obtained using the abovementioned procedure are of only 1%.     

Comparison of observed hmF2 and IRI 2007 model with M(3000)F2 estimation of hmF2 at low solar activity for an equatorial station

This is to investigate ways of improving the Equatorial F2-layer peak heights estimated from M(3000)F2 ionosonde data measured using the Ionospheric Prediction Service (IPS-42) sounder at Ouagadougou, Burkina Faso (Latitude +12.4°N, Longitude +1.5°W, Dip latitude +5.9°N) during a low solar activity year (1995). For this purpose, we have compared the observed h_mF2 (h_mF2_obs) deduced using an algorithm from scaled virtual heights of quiet day ionograms and the predicted h_mF2 values which is given by the IRI 2007 model (h_mF2_{IRI 2007}) with the ionosonde measured M(3000)F2 estimation of the h_mF2 values (h_mF2_est) respectively. The correlation coefficients R² for all the seasons were found to range from 0.259 to 0.692 for h_mF2_obs values, while it ranges from 0.551 to 0.875 for the h_mF2_{IRI 2007} values. During the nighttime, estimated h_mF2 (h_mF2_est) was found to be positively correlated with the h_mF2_obs values by the post-sunset peak representation which is also represented by the h_mF2_{IRI 2007} values. We also investigated the validity of the h_mF2_est values by finding the percentage deviations when compared with the h_mF2_obs and h_mF2_{IRI 2007}.     

Variability of the ionospheric electron density at fixed heights and validation of IRI-2007 profile’s prediction at Ilorin

A study on the variability of the equatorial ionospheric electron density was carried out at fixed heights below the F2 peak using one month data for each of high and low solar activity periods. The data used for this study were obtained from ionograms recorded at Ilorin, Nigeria, and the study covers height range from 100 km to the peak of the F2 layer for the daytime hours and height range from 200 km to the peak of the F2 layer for the nighttime hours. The results showed that the deviation of the electron density variation from simple Chapman variation begins from an altitude of about 200 km for the two months investigated. Daytime minimum variability of between 2.7% and 9.0% was observed at the height range of about 160 and 200 km during low solar activity (January 2006) and between 3.7% and 7.8% at the height range of 210 and 260 km during high solar activity (January 2002). The nighttime maximum variability was observed at the height range of 210 and 240 km at low solar activity and at the height range of 200 and 240 km at high solar activity. A validation of IRI-2007 model electron density profile’s prediction was also carried out. The results showed that B0 option gives a better prediction around the noontime.     

Improvements in bottomside electron density definition in the Autoscala program

Some improvements introduced in the Autoscala program are presented. They include improvements in E valley modeling of the electron density profile N_e(h), and in the link between the E valley and bottom-side F regions. An abrupt variation in N_e(h) generated by the previous version of Autoscala under night conditions has been eliminated.A series of ionograms recorded by the Millstone Hill digisonde (42.6°, 288.5°) were automatically interpreted by the previous version of Autoscala and by the new one. Data from Incoherent Scatter Radar (ISR) were used to comparatively assess the performance of the two versions. For this purpose, the root mean square errors (RMSEs) of the N_e(h) provided by Autoscala were calculated relative to the corresponding values provided by ISR.A more accurate overall modeling of N_e(h) was achieved by the new Autoscala version (RMSE = 0.51 MHz for the new version against RMSE = 0.67 MHz for the previous one).     

Efficacy of oxygen-supplying capacity of Azolla in a controlled life support system

Azolla shows high growth and propagation rates, strong photosynthetic O₂-releasing ability and high nutritional value. It is suitable as a salad vegetable and can be cultured on a multi-layered wet bed. Hence, it possesses potential as a fresh vegetable, and to release O₂ and absorb CO₂ in a Controlled Ecological Life Support System in space. In this study, we investigated the O₂-providing characteristics of Azolla in a closed chamber under manned, controlled conditions to lay a foundation for use of Azolla as a biological component in ground simulation experiments for space applications. A closed test chamber, representing a Controlled Ecological Life Support System including an Azolla wet-culture device, was built to measure the changes in atmospheric O₂ and CO₂ concentrations inside the chamber in the presence of coexisting Azolla, fish and men. The amount of O₂ consumed by fish was 0.0805–0.0831 L kg⁻¹ h⁻¹ and the level of CO₂ emission was 0.0705–0.0736 L kg⁻¹ h⁻¹; O₂ consumption by the two trial volunteers was 19.71 L h⁻¹ and the volume of respiration-released CO₂ was 18.90 L h⁻¹. Under 7000–8000 Lx artificial light and Azolla wet-culture conditions, human and fish respiration and Azolla photosynthesis were complementary, thus the atmospheric O₂ and CO₂ concentrations inside chamber were maintained in equilibrium. The increase in atmospheric CO₂ concentration in the closed chamber enhanced the net photosynthesis efficiency of the Azolla colony. This study showed that Azolla has strong photosynthetic O₂-releasing ability, which equilibrates the O₂ and CO₂ concentrations inside the chamber in favor of human survival and verifies the potential of Azolla for space applications.     

Estimation of the Love and Shida numbers: h2, l2 using SLR data for the low satellites

In this paper we present results for the global elastic parameters: Love number h₂ and Shida number l₂ derived from the analysis of Satellite Laser Ranging (SLR) data. SLR data for the two low satellites STELLA (H = 800 km) and STARLETTE (H = 810 km) observed during 2.5 years from January 3, 2005 until July 1, 2007 with 18 globally distributed ground stations were analyzed. The analysis was done separately for the two satellites. We do a sequential analysis and study the stability and convergence of the estimates as a function of length of the data set used.     

Equatorial vertical E × B drift velocities inferred from ionosonde measurements over Ouagadougou and the IRI-2007 vertical ion drift model

F-region vertical plasma drift velocities were deduced from the hourly hmF2 values acquired from ionogram data over a near dip equatorial station Ouagadougou (12.4°N, 358.5°E, dip angle 5.9°N) in Africa. Our results are compared against the global empirical model of Scherliess and Fejer (1999) incorporated in the IRI model (IRI-2007) for 1600 to 0800 LT from 1 year of data during sunspot maximum year of 1989 (yearly average solar flux intensity, F10.7 = 192) corresponding to the peak phase of solar cycle 22, under magnetically quiet conditions. The drifts are entirely downward between 2000 and 0500 LT bin for both techniques and the root mean square error (RMSE) between the modeled and the ionosonde vertical plasma drifts during these periods is 3.80, 4.37, and 4.74 m/s for June solstice, December solstice and equinox, respectively. Ouagadougou average vertical drifts show evening prereversal enhancement (PRE) velocity peaks (V_ZP) of about 16, 14, and 17 m/s in June solstice, December solstice, and equinox, respectively, at 1900–2000 LT; whereas global empirical model average drifts indicate V_ZP of approximately 33 m/s (June solstice), 29 m/s (December solstice), and 50 m/s (equinox) at 1800 LT. We find very weak and positive correlation (+0.10376) between modeled V_ZP versus F10.7, while ionosonde V_ZP against F10.7 gives worst and opposite correlation (−0.05799). The results also show that modeled V_ZP–Ap indicates good and positive correlation (+0.64289), but ionosonde V_ZP–Ap exhibits poor and negative correlation (−0.22477).     

Cowpeas and pinto beans: Performance and yields of candidate space crops in the laboratory biosphere closed ecological system

An experiment utilizing cowpeas (Vigna unguiculata L.), pinto beans (Phaseolus vulgaris L.) and Apogee ultra-dwarf wheat (Triticum sativa L.) was conducted in the soil-based closed ecological facility, Laboratory Biosphere, from February to May 2005. The lighting regime was 13 h light/11 h dark at a light intensity of 960 μmol m⁻² s⁻¹, 45 mol m⁻² day⁻¹ supplied by high-pressure sodium lamps. The pinto beans and cowpeas were grown at two different planting densities. Pinto bean production was 341.5 g dry seed m⁻² (5.42 g m⁻² day⁻¹) and 579.5 dry seed m⁻² (9.20 g m⁻² day⁻¹) at planted densities of 32.5 plants m⁻² and 37.5 plants m⁻², respectively. Cowpea yielded 187.9 g dry seed m⁻² (2.21 g m⁻² day⁻¹) and 348.8 dry seed m⁻² (4.10 g m⁻² day⁻¹) at planted densities of 20.8 plants m⁻² and 27.7 plants m⁻², respectively. The crop was grown at elevated atmospheric carbon dioxide levels, with levels ranging from 300–3000 ppm daily during the majority of the crop cycle. During early stages (first 10 days) of the crop, CO₂ was allowed to rise to 7860 ppm while soil respiration dominated, and then was brought down by plant photosynthesis. CO₂ was injected 27 times during days 29–71 to replenish CO₂ used by the crop during photosynthesis. Temperature regime was 24–28 °C day/deg 20–24 °C night. Pinto bean matured and was harvested 20 days earlier than is typical for this variety, while the cowpea, which had trouble establishing, took 25 days more for harvest than typical for this variety. Productivity and atmospheric dynamic results of these studies contribute toward the design of an envisioned ground-based test bed prototype Mars base.     

The COMPLEIK subroutine of the IONORT-ISP system for calculating the non-deviative absorption: A comparison with the ICEPAC formula

The present paper proposes to discuss the ionospheric absorption, assuming a quasi-flat layered ionospheric medium, with small horizontal gradients. A recent complex eikonal model (Settimi et al., 2013b) is applied, useful to calculate the absorption due to the ionospheric D-layer, which can be approximately characterized by a linearized analytical profile of complex refractive index, covering a short range of heights between h₁ = 50 km and h₂ = 90 km. Moreover, Settimi et al. (2013c) have already compared the complex eikonal model for the D-layer with the analytical Chapman’s profile of ionospheric electron density; the corresponding absorption coefficient is more accurate than Rawer’s theory (1976) in the range of middle critical frequencies. Finally, in this paper, the simple complex eikonal equations, in quasi-longitudinal (QL) approximation, for calculating the non-deviative absorption coefficient due to the propagation across the D-layer are encoded into a so called COMPLEIK (COMPLex EIKonal) subroutine of the IONORT (IONOspheric Ray-Tracing) program ( Azzarone et al., 2012). The IONORT program, which simulates the three-dimensional (3-D) ray-tracing for high frequencies (HF) waves in the ionosphere, runs on the assimilative ISP (IRI-SIRMUP-P) discrete model over the Mediterranean area ( Pezzopane et al., 2011). As main outcome of the paper, the simple COMPLEIK algorithm is compared to the more elaborate semi-empirical ICEPAC formula (Stewart, undated), which refers to various phenomenological parameters such as the critical frequency of E-layer. COMPLEIK is reliable just like the ICEPAC, with the advantage of being implemented more directly. Indeed, the complex eikonal model depends just on some parameters of the electron density profile, which are numerically calculable, such as the maximum height.     

Response of low latitude D-region ionosphere to the total solar eclipse of 22 July 2009 deduced from ELF/VLF analysis

Response of the D-region of the ionosphere to the total solar eclipse of 22 July 2009 at low latitude, Varanasi (Geog. lat., 25.27° N; Geog. long., 82.98° E; Geomag. lat. = 14° 55’ N) was investigated using ELF/VLF radio signal. Tweeks, a naturally occurring VLF signal and radio signals from various VLF navigational transmitters are first time used simultaneously to study the effect of total solar eclipse (TSE). Tweeks occurrence is a nighttime phenomena but the obscuration of solar disc during TSE in early morning leads to tweek occurrence. The changes in D-region ionospheric VLF reflection heights (h) and electron density (n_e: 22.6–24.6 cm⁻³) during eclipse have been estimated from tweek analysis. The reflection height increased from ∼89 km from the first occurrence of tweek to about ∼93 km at the totality and then decreased to ∼88 km at the end of the eclipse, suggesting significant increase in tweek reflection height of about 5.5 km during the eclipse. The reflection heights at the time of totality during TSE are found to be less by 2–3 km as compared to the usual nighttime tweek reflection heights. This is due to partial nighttime condition created by TSE. A significant increase of 3 dB in the strength of the amplitude of VLF signal of 22.2 kHz transmitted from JJI-Japan is observed around the time of the total solar eclipse (TSE) as compared to a normal day. The modeled electron density height profile of the lower ionosphere depicts linear variation in the electron density with respect to solar radiation as observed by tweek analysis also. These low latitude ionospheric perturbations on the eclipse day are discussed and compared with other normal days.     

Physical and model interpretation of HF radio propagation on the St. Petersburg–Longyearbyen (Svalbard) path

HF radio wave observations have been carried out with an oblique ionospheric sounding (OIS) method on the radio path from St. Petersburg to Longyearbyen (Svalbard), and experimental ionograms were obtained for December 2001. These ionograms have been analysed to investigate the impact of the main ionospheric trough (MIT) and magnetic disturbances on the signals on this path. The observations during weakly disturbed (K_р = 2) magnetic conditions on 14–15 December 2001 were compared with predictions from ray-tracing through a numerical model of the ionosphere. The ray-tracing computer program synthesizes the OIS ionograms by means of the “shooting method”. This method calculates trajectories of HF radio waves for different values of elevation angle and transmission frequency. There was a variety of calculated trajectories, from which we choose those which reach the receiver, and the selected paths provide a synthesis of the oblique ionograms. To simulate HF radio wave propagation, we apply a three-dimensional distribution of the electron density calculated with the mathematical model of the high-latitude ionosphere developed in the Polar Geophysical Institute (PGI). These numerical simulations permit us to interpret specific peculiarities of the OIS data such as abnormal propagation modes, increased delays of signals, enhanced MOF (maximum observed frequency) values etc. New results of the study are summarised as follows. (1) An unusual feature of the propagation along the path is the change of propagation mechanism during substorms on entering a path midpoint (or 1-hop reflection point) to the MIT. (2) Even weak substorms, having the distinguished intensities, lead to the appearance of different types of irregularities observed by the CUTLASS radar and therefore to the different propagation modes and F2MOF values. (3) The PGI model of the ionosphere was first used for ray-tracing at high latitudes. The model results are basically in a good qualitative agreement with experimental observations. This model provides the satisfactory agreement between the calculated and experimental F2MOF values while not correctly representing the fine structure of the experimental OIS ionograms at night. An agreement between the calculated and experimental data is better for day and evening hours than at night.     

Gas exchange rates of potato stands for bioregenerative life support

Plants can provide a means for removing carbon dioxide (CO₂) while generating oxygen (O₂) and clean water for life support systems in space. To study this, 20 m² stands of potato (Solanum tuberosum L.) plants were grown in a large (113 m³ vol.), atmospherically closed chamber. Photosynthetic uptake of CO₂ by the stands was detected about 10 DAP (days after planting), after which photosynthetic rates rose rapidly as stand ground cover and total light interception increased. Photosynthetic rates peaked ca. 50 DAP near 45 μmol CO₂ m⁻² s⁻¹ under 865 μmol m⁻² s⁻¹ PPF (average photosynthetic photon flux), and near 35 μmol CO₂ m⁻² s⁻¹ under 655 μmol m⁻² s⁻¹ PPF. Short term changes in PPF caused a linear response in stand photosynthetic rates up to 1100 μmol m⁻² s⁻¹ PPF, with a light compensation point of 185 μmol m⁻² s⁻¹ PPF. Comparisons of stand photosynthetic rates at different CO₂ concentrations showed a classic C₃ response, with saturation occurring near 1200 μmol mol⁻¹ CO₂ and compensation near 100 μmol mol⁻¹ CO₂. In one study, the photoperiod was changed from 12 h light/12 h dark to continuous light at 58 DAP. This caused a decrease in net photosynthetic rates within 48 h and eventual damage (scorching) of upper canopy leaves, suggesting the abrupt change stressed the plants and/or caused feedback effects on photosynthesis. Dark period (night) respiration rates increased during early growth as standing biomass increased and peaked near 9 μmol CO₂ m⁻² s⁻¹ ca. 50 DAP, after which rates declined gradually with age. Stand transpiration showed a rapid rise with canopy ground cover and peaked ca. 50 DAP near 8.9 L m⁻² d⁻¹ under 860 μmol m⁻² s⁻¹ PPF and near 6.3 L m⁻² d⁻¹ under 650 μmol m⁻² s⁻¹ PPF. Based on the best photosynthetic rates from these studies, approximately 25 m² of potato plants under continuous cultivation would be required to support the CO₂ removal and O₂ requirements for one person.     

Tolerance of chufa (Cyperus esculentus L.) plants,representing the higher plant compartment in bioregenerative life support systems,to super-optimal air temperatures

Plants intended to be included in the photosynthesizing compartment of the bioregenerative life support system (BLSS) need to be studied in terms of both their production parameters under optimal conditions and their tolerance to stress factors that might be caused by emergency situations. The purpose of this study was to investigate tolerance of chufa (Cyperus esculentus L.) plants to the super-optimal air temperature of 45 ± 1 °C as dependent upon PAR (photosynthetically active radiation) intensity and the duration of the exposure to the stress factor. Chufa plants were grown hydroponically, on expanded clay, under artificial light. The nutrient solution was Knop’s mineral medium. Until the plants were 30 days old, they had been grown at 690 μmol m⁻² s⁻¹ PAR and air temperature 25 °C. Thirty-day-old plants were exposed to the temperature 45 °C for 6 h, 20 h, and 44 h at PAR intensities 690 μmol m⁻² s⁻¹ and 1150 μmol m⁻² s⁻¹. The exposure to the damaging air temperature for 44 h at 690 μmol m⁻² s⁻¹ PAR caused irreversible damage to PSA, resulting in leaf mortality. In chufa plants exposed to heat shock treatment at 690 μmol m⁻² s⁻¹ PAR for 6 h and 20 h, respiration exceeded photosynthesis, and CO₂ release in the light was recorded. Functional activity of photosynthetic apparatus, estimated from parameters of pulse-modulated chlorophyll fluorescence in Photosystem 2 (PS 2), decreased 40% to 50%. After the exposure to the stress factor was finished, functional activity of PSA recovered its initial values, and apparent photosynthesis (P_apparent) rate after a 20-h exposure to the stress factor was 2.6 times lower than before the elevation of the temperature. During the first hours of plant exposure to the temperature 45 °C at 1150 μmol m⁻² s⁻¹ PAR, respiration rate was higher than photosynthesis rate, but after 3–4 h of the exposure, photosynthetic processes exceeded oxidative ones and CO₂ absorption in the light was recorded. At the end of the 6-h exposure, P_apparent rate was close to that recorded prior to the exposure, and no significant changes were observed in the functional activity of PSA. At the end of the 20-h exposure, P_apparent rate was close to its initial value, but certain parameters of the functional activity of PSA decreased 25% vs. their initial values. During the repair period, the parameters of external gas exchange recovered their initial values, and parameters of pulse-modulated chlorophyll fluorescence were 20–30% higher than their initial values. Thus, exposure of chufa plants to the damaging temperature of the air for 20 h did not cause any irreversible damage to the photosynthetic apparatus of plants at either 690 μmol m⁻² s⁻¹ or 1150 μmol m⁻² s⁻¹ PAR, and higher PAR intensity during the heat shock treatment enhanced heat tolerance of the plants.     

Study on O2 generation and CO2 absorption capability of four co-cultured salad plants in an enclosed system

The ability to generate O₂ and absorb CO₂ of several co-cultured vegetable plants in an enclosed system was studied to provide theoretical reference for the future man-plant integrated tests. Four kinds of salad plants (Lactuca sativa L. var. Dasusheng, Lactuca sativa L. var. Youmaicai, Gynura bicolor and Cichorium endivia L.) were grown in the CELSS Integration Test Platform (CITP). The environmental factors including O₂ and CO₂ concentration were continuously monitored on-line and the plant biomass was measured at the end of the test. The changing rules of O₂ and CO₂ concentration in the system were basically understood and it was found that the O₂ generated by the plants could satisfy the respiratory needs of 1.75 persons by calculation. It was also found that the plants could absorb the CO₂ breathed out by 2 persons when the light intensity was raised to 550 mmol m⁻² s⁻¹ PPF. The results showed that the co-cultured plants hold good compatibility and excellent O₂-generating and CO₂-absorbing capability. They could also supply some fresh edible vegetable for a 2-person crew.