Significance of rhizosphere microorganisms in reclaiming water in a CELSS.

Plant-microbe interactions, such as those of the rhizosphere, may be ideally suited for recycling water in a Controlled Ecological Life Support System (CELSS). The primary contaminant of waste hygiene water will be surfactants or soaps. We identified changes in the microbial ecology in the rhizosphere of hydroponical1y grown lettuce during exposure to surfactant. Six week old lettuce plants were transferred into a chamber with a recirculating hydroponic system. Microbial density and population composition were determined for the nutrient solution prior to introduction of plants and then again with plants prior to surfactant addition. The surfactant Igepon was added to the recirculating nutrient solution to a final concentration of 1.0 g L-1. Bacteria density and species diversity of the solution were monitored over a 72-h period following introduction of Igepon. Nine distinct bacterial types were identified in the rhisosphere; three species accounted for 87% of the normal rhizosphere population. Microbial cell number increased in the presence of Igepon, however species diversity declined. At the point when Igepon was degraded from solution, diversity was reduced to only two species. Igepon was found to be degraded directly by only one species found in the rhizosphere. Since surfactants are degraded from the waste hygiene water within 24 h, the potential for using rhizosphere bacteria as a waste processor in a CELSS is promising.     

Plant cell plasma membrane structure and properties under clinostatting.

Structural-functional organization of plasma membrane of pea roots seedling was investigated by methods of chemiluminescence, fluorescence probes, chromatography and freeze-fracture studies under normal conditions and clinostatting. Phase character of lipid peroxidation intensity was fixed. The initial phase of this process is characterized by lipid peroxidation decreasing with its next induction. The primary changes depending on free-radical mechanisms of lipid peroxidation were excellently revealed by chemiluminescence. Plasmalemma microviscosity increased on the average of 15-20% under microgravity at the initial stages of its phenomenon. There were major changes of phosphatidilcholine and phosphatidilethanolamine contents. The total quantity of phospholipids remained rather stable. Changes of phosphatide acid concentration point to degradation and phospholipids biosynthesis. There were increases of unsaturated fatty acids mainly at the expense of linoleic and linolenic acids and also a decrease of saturated fatty acid content at the expense of palmitic and stearic acids. Unsaturation index of fatty acids increased as well. On the whole fatty acid composition was variable in comparison with phospholipids. Probably it is one of mechanisms of maintaining of microviscosity within definite limits. Considerable structural changes in organization of plasmalemma protein-lipid complex were not revealed by the freeze-fracture studies.     

The effects of space flight on some rat liver enzymes regulating carbohydrate and lipid metabolism

We have examined, in the livers of rats carried aboard the Cosmos 936 biosatellite, the activities of about 30 enzymes concerned with carbohydrate and lipid metabolism. In addition to the enzyme studies, the levels of glycogen and of the individual fatty acids in hepatic lipids were determined. Livers from flight and ground control rats at recovery (R₀) and 25 days after recovery (R₂₅) were used for these analyses.

For all parameters measured, the most meaningful comparisons are those made between flight stationary (FS) and flight centrifuged (FC) animals at R₀. When these two groups of flight rats were compared at R₀, statistically significant decreases in the activity levels of glycogen phosphorylase, -glycerol phosphate acyl transferase, diglyceride acyl transferase, aconitase and 6-phosphogluconate dehydrogenase and an increase in the palmitoyl CoA desaturase were noted in the weightless group (FS). The significance of these findings was strengthened by the fact that all enzyme activities showing alterations at R₀ returned to normal 25 days postflight. When liver glycogen and total fatty acids of the two sets of flight animals were determined, significant differences that could be attributed to reduced gravity were observed. The weightless group (FS) at R₀ contained, on the average, more than twice the amount of glycogen than did the centrifuged controls (FC) and a remarkable shift in the ratio of palmitate to palmitoleate was noted. These metabolic alterations, both in enzyme levels and in hepatic constituents, appear to be characteristic of the weightless condition. Our data seem to justify the conclusion that centrifugation during flight is equivalent to terrestrial gravity.     

The effect of artificial gravity on plasma and tissue lipids in rats: The Cosmos 936 experiment

Plasma and tissue lipids in male SPF Wistar rats flown for 18.5 days aboard the Cosmos 936 biosatellite were analyzed. One group of rats was subjected to artificial gravity by use of a centrifuge during the flight. An experiment simulating known space flight factors other than weightlessness was done on Earth. An increase of total cholesterol in plasma, of nonesterified fatty acids in plasma and brown adipose tissue, of triacylglycerols in plasma, liver, thymus and bone marrow was noted several hours after biosatellite landing. Smaller changes were observed in the terrestrial control experiment. With the exception of triacylglycerol accumulation in bone marrow, these increases disappeared 25 days after biosatellite landing. Exposing the rats aboard the biosatellite to artificial gravity was beneficial in the sense that such exposure inhibited the phospholipid and triacylglycerol increase in plasma and inhibited the increase of triacylglycerol in liver and especially in bone marrow.     

Transpiration during life cycle in controlled wheat growth.

We use a previously-developed model of wheat growth, which was designed for convenient incorporation into system-level models of advanced space life support systems. We apply the model to data from an experiment that grew wheat under controlled conditions and measured fresh biomass and cumulated transpiration as a function of time. We examine the adequacy of modeling the transpiration as proportional to the inedible biomass and an age factor, which varies during the life cycle. Results indicate that during the main phase of vegetative growth in the first half of the life cycle, the rate of transpiration per unit mass of inedible biomass is more than double the rate during the phase of grain development and maturation during latter half of the life cycle.     

Chemical evolution of RNA under hydrothermal conditions and the role of thermal copolymers of amino acids for the prebiotic degradation and formation of RNA.

The roles of thermal copolymers of amino acids (TCAA) were studied for the prebiotic degradation of RNA. A weak catalytic ability of TCAA consisted of Glu, L-Ala, L-Val, L-Glu, L-Asp, and optionally L-His was detected for the cleavage of the ribose phosphodiester bond of a tetranucleotide (5'-dCrCdGdG) in aqueous solution at 80 degees C. The rate constants of the disappearance of 5'-dCrCdGdG were determined in aqueous solutions using different pH buffer and TCAA. The degradation rates were enhanced 1.3-3.0 times in the presence of TCAA at pH 7.5 and 8.0 at 80 degrees C, while the hydrolysis of oligoguanylate (oligo(G)) was accelerated about 1.6 times at pH 8.0. A weak inhibitory activity for the cleavage of oligo(G) was detected in the presence of 0.055 M TCAA-Std. On the other hand, our recent study on the influences of TCAA for the template-directed reaction of oligo(G) on a polycytidylic acid template showed that TCAA has an acceleration activity for the degradation of the activated nucleotide monomer and an acceleration activity for the formation of G5' ppG capped oligo(G). This series of studies suggest that efficient and selective catalytic or inhibitory activities for either the degradation or formation of RNA under hydrothermal conditions could have hardly emerged from the simple thermal condensation products of amino acids. A scenario is going to be deduced on the chemical evolution of enzymatic activities and RNA molecules concerning hydrothermal earth conditions.     

Stable isotope analysis at the molecular level: a new approach for determining the origins of amino acids in the Murchison meteorite.

A combined gas chromatography/isotope ratio mass spectrometry (GC/IRMS) method has been developed that permits the direct stable carbon isotope analysis of N(O)-trifluoroacetyl-isopropyl esters of individual amino acids and their respective enantiomers at nanomole abundances. Calculation of the original delta 13C values of the amino acids is accomplished via a correction for the carbon introduced during the derivatization process. Previous GC/IRMS analyses of individual amino acids in the non-hydrolyzed water extract of an interior sample of a Murchison meteorite stone revealed an enrichment in 13C relative to terrestrial organic matter, in agreement with previous findings for bulk extracts. The range of amino acid delta 13C values (+5 to +30%, PDB) suggests possible kinetic effects during synthesis. In this study, an apparent kinetic isotope effect was also observed for the amino acid products of a spark discharge experiment. These preliminary results are supportive of a similar mechanism for the abiotic synthesis of amino acids in the Murchison meteorite.     

第23和24太阳活动周高纬地磁感应电流分布特性

基于高纬度芬兰Mäntsälä地区近两个太阳活动周期（1999—2017年）天然气传输管道的地磁感应电流（GIC,I_GIC）观测数据,统计研究了GIC扰动的分布特征以及强GIC扰动与磁暴和地磁亚暴的相关性.研究发现:95.83%时间段的GIC强度分布在0～1A之间.定义:若某个时间段|I_GIC|_max> 1A,则认为发生GIC扰动;|I_GIC|_max>10A,则认为发生强GIC扰动事件.GIC扰动在磁地方时夜侧附近发生的概率最高,这主要与地磁亚暴发生期间电离层电流最剧烈的变化发生在磁地方时夜侧附近有关;强GIC扰动经常爆发式出现,且都发生在磁暴期间,但大多数磁暴并不伴随强GIC扰动事件发生.磁暴急始驱动的强GIC扰动事件较少,由磁层压缩引起地磁场突然增强驱动的强GIC扰动事件持续时间较短;强GIC扰动事件主要发生在磁暴主相和恢复相,由环电流变化驱动的强GIC扰动事件一般持续时间较长且强度较大.      

Impact of heliogeophysical disturbances on ionospheric HF channels

The article presents the results of the observation of a strong magnetic storm and two X-ray flares during the summer solstice in 2015, and their impact on the HF signals characteristics in ionospheric oblique sounding. It was found that the negative phase of the magnetic storm led to a strong degradation of the ionospheric channel, ultimately causing a long blackout on paths adjacent to subauroral latitudes. On mid-latitude paths, the decrease in 1FMOF reached ～50% relative to the average values for the quiet ionosphere. It is shown that the propagation conditions via the sporadic Es layer during the magnetic storm on a subauroral path are substantially better than those for F-mode propagation via the upper ionosphere. The delay of the sharp decrease in 1FMOF during the main phase of the magnetic storm allowed us to determine the propagation velocity of the negative phase disturbances (～100 m/s) from subauroral to mid-latitude ionosphere along two paths: Lovozero – Yoshkar-Ola and Cyprus – Nizhny Novgorod. It is shown that both the LOF and the signal/noise ratio averaged over the frequency band corresponding to the propagation mode via the sporadic Es layer correlate well with the auroral AE index. Using an over-the-horizon chirp radar with a bistatic configuration on the Cyprus – Rostov-on-Don path, we located small-scale scattering irregularities responsible for abnormal signals in the region of the equatorial boundary of the auroral oval.     

Nighttime ionosphere–thermosphere coupling observed during an intense geomagnetic storm

The electrodynamics of the ionosphere in the tropical region presents various scientific aspects, which remain subject of intensive investigations and debates by the scientific community. During the year 2002, in a joint project between the Universidade do Vale do Paraíba (UNIVAP) and Universidade Luterana do Brasil (ULBRA), a chain of three Canadian Advanced Digital Ionosondes (CADIs) was established nearly along the geomagnetic meridian direction, for tropical ionospheric studies, such as, changes and response due to geomagnetic disturbances and thermosphere–ionosphere coupling and the generation and dynamics of ionospheric irregularities, in the Brazilian sector. The locations of the three ionosondes stations are São José dos Campos (23.2°S, 45.9°W, dip latitude 17.6°S – under the southern crest of equatorial ionospheric anomaly), Palmas (10.2°S, 48.2°W, dip latitude 5.5°S – near the magnetic equator) and Manaus (2.9°S, 60.0°W, dip latitude 6.4°N – between the geographic and geomagnetic dip equators). It should be pointed out that Palmas and Manaus are located on the opposite sides of the magnetic equator but both are south of the geographic equator. The three CADIs work in time-synchronized mode and obtain ionograms every 5 min. This configuration of the ionospheric sounding stations allowed us to study the F-region dynamics during geomagnetically disturbed period in the meridional direction. Just after the installation and testing of the three CADIs, on September 05, 2002 a coronal mass ejection (CME) left the Sun and about 2 days after the CME left the Sun, it reached the Earth’s magnetosphere and complex and multi step events took place during the period September 07–09. In the study we note that the equatorial stations located north (Manaus, dip latitude 6.4°N) and south (Palmas, dip latitude 5.5°S) of the dip equator presented significant F-layer height asymmetries during the storm main phase. In addition, the low-latitude station SJC (dip latitude 17.6°S) presented decrease in the F-layer densities (negative phase), whereas Palmas presented increase in the F-layer densities (positive phase) during the main phase. This was followed by positive phase at both the stations. During the first night of the recovery phase a strong formation and evolution of large-scale ionospheric irregularities (equatorial spread-F (ESF)) was observed, but on the second night of the recovery phase, there was strong and almost simultaneous sporadic E (Es) formation at all three stations. During the presence of Es, spread-F formation is not observed, indicating the suppression of spread-F, possibly by sporadic E.     

The evolution of nucleotides.

There has been much speculation about the types of molecules that were present in the first living forms. Recent discoveries show that RNA is a more versatile molecule than was previously believed, but whether it was ever able, for example, to synthesize its own monomers from available precursors is not yet known. If amino acids coexisted with nucleosides on the prebiotic Earth, then it seems likely that these two classes of molecules would have interacted with each other. We have been studying oligonucleotide-directed peptide bond formation, and during this work we discovered that aminoacylation of the internal 2'-hydroxyl groups of RNA occurred stereoselectively. Investigation of the mechanism of this reaction has been aided by the use of 3'-inosine methyl phosphate (as a simplified model for a dinucleoside monophosphate) and proton nmr spectroscopy of t-butoxycarbonyl-alanyl esters of nucleosides as models for the transition state of the aminoacylation reaction itself.     

Ionospheric parameters in the European sector during the magnetic storm of August 25–26, 2018

Variations of ionospheric parameters Total Electron Content (TEC) by GNSS, critical frequency (foF2) by vertical sounding and electron density (Ne) by low-altitude satellite were studied at high, mid and low latitudes of the European sector during the magnetic storm of August 25–26, 2018. During the main phase of the storm the ionospheric F2-layer was under the positive disturbance at mid and low latitudes. Then the transition from the positive to negative ΔfoF2 values occurred at all latitudes. The recovery phase was characterized by negative ionospheric disturbance at all latitudes. This is due to the decrease of thermospheric O/N2 ratio during the recovery phase of the storm. The intense Es layers screened the reflections from the F2-layer on August 26th at high and at low latitudes but at different times. Some blackouts occurred due to the high absorption level at high latitudes. In general, foF2 and TEC data were highly correlated. The major Ne changes were at the low latitudes. In general, Ne data confirmed the ionospheric dynamics revealed with foF2 and TEC.     

Do we understand the inner magnetotail dynamics during substorms?

Substorm evolution of the near-Earth (|X|<15 R_E) plasma sheet has been emphasized recently because the inner tail is thought to link closely to the substorm auroral activity in the ionosphere during the early stage of substorms. In this paper, we discuss how the inner tail substorm phenomena during the late substorm growth phase and early expansion phase are accounted for by the two prevailing substorm models, namely, the near-Earth neutral line model and the current disruption model. We find that the late growth phase features are more satisfactorily accounted for by the current disruption model than by the near-Earth neutral line model. In addition, detailed observations on current disruption show evidence inconsistent with the proposed idea of dipolarization being due to plasma flow braking from reconnection in the mid-tail region, which poses a difficulty to the near-Earth neutral line model as well.     

Hard X-ray and radio investigations prior to or during the impulsive phase of solar flares

The comparative study of radiation in the different spectral ranges, including X-ray and radio observations, can establish constraints for the electron acceleration/injection mechanisms. This paper will focus on the activity prior and during the impulsive phase of solar flares. Observations give evidence for electron acceleration prior the impulsive phase. The association between type III groups and hard X-ray bursts becomes closer with increasing starting frequency of the former observed during the impulsive phase. It is shown that pure type III burst groups, when they are X-ray associated, do not correspond to an intense X-ray emission. At the opposite, the type III/V events can be associated with strong X-ray emission. Radioheliograph observations bring constraints on the geometry of the injection/acceleration site.     

Storm time spatial variations in TEC during moderate geomagnetic storms in extremely low solar activity conditions (2007–2009) over Indian region

The total electron content (TEC) measurements from a network of GPS receivers were analyzed to investigate the storm time spatial response of ionosphere over the Indian longitude sector. The GPS receivers from the GPS Aided Geo Augmented Navigation (GAGAN) network which are uniquely located around the ∼77°E longitude are used in the present study so as to get the complete latitudinal coverage from the magnetic equator to low mid-latitude region. We have selected the most intense storms but of moderate intensity (−100 nT < Dst < −50 nT) which occurred during the unusually extremely low solar activity conditions in 2007–2009. Though the storms are of moderate intensity, their effects on equatorial to low mid-latitude ionosphere are found to be very severe as TEC deviations are more than 100% during all the storms studied. Interesting results in terms of spatial distribution of positive/negative effects during the main/early recovery phase of storms are noticed. The maximum effect was observed at crest region during two storms whereas another two storms had maximum effect near the low mid-latitude region. The associated mechanisms like equatorial electrodynamics and neutral dynamics are segregated and explained using the TIMED/GUVI and EEJ data during these storms. The TEC maps are generated to investigate the storm time development/inhibition of equatorial ionization anomaly (EIA).     

Initial phase of mid-latitude aurora during strong geomagnetic storms

Analysing the initial mid-latitude aurora phase during strong geomagnetic storms we found that the initial phase of the mid-latitude aurorae observed at 630 nm emission during the strong geomagnetic storms on March 24, 1991, April 6, 2000, October 30 and November 20, 2003 is characterized by a short (∼1 h) wave-like disturbance. This disturbance corresponds to the beginning of main phase of the magnetic storms. The marked effect of the mid-latitude aurorae is analyzed using data on magnetosphere and ionosphere conditions in observation periods. The features of the dynamics of the 630 nm emission intensity and its connection with the dynamics of magnetospheric–ionospheric structures are considered. Possible excitation mechanisms of the atomic oxygen emission (630 nm) during these disturbances are discussed.     

The parameters of shock acoustic waves generated during rocket launches

We investigate the form and dynamics of shock acoustic waves (SAW) generated during the launching of rockets Proton and Soyuz from the Baikonur cosmodrome in 1998–2000. In spite of the difference of geophysical conditions, the ionospheric response for all launchings had the character of an N-wave with a period of about 300 s and with an amplitude far exceeding background fluctuations. The angle of elevation of the SAW wave vector varies from 30° to 65°, and the SAW phase velocity (800–1200 m/s) approaches the sound velocity at heights of the ionospheric F region maximum. The position of the SAW source, inferred by neglecting refraction corrections, corresponds to the segment of the rocket path at a distance no less than 700–900 km from the launch pad, which is consistent with the estimated delay time of SAW source triggering (250–300 s).     

Seasonal variations of GPS derived TEC at three different latitudes of the southern hemisphere during geomagnetic storms

Data from the archive of the International GNSS Services (IGS) were used to study the seasonal variations of Total Electron Content (TEC) over three stations located at different latitudes in the southern hemisphere during the geomagnetic storms of 11 January, 6 April, 8 June, and 13 October 2000, representing storms that occurred in summer, autumn equinox, winter and spring equinox, respectively. The percentage TEC deviation with respect to reference values differs substantially from season to season. A strong seasonal anomaly and clear equinoctial asymmetry in TEC response to the storms were observed. Weak and short-lived positive TEC deviations as well as strong and long-lasting negative trends were observed in summer storm during the main and recovery phases respectively over the high and low latitudes whereas in winter storm, the highest positive TEC deviations was recorded during the main phase over the entire latitudes. TEC enhancement dominated all the stations during the autumn (March) equinox storm while TEC depletion was majorly observed during the spring (September) equinox. All these variations find their explanations in the thermospheric composition change and circulation. Future work with direct or modeled measurement of atomic Oxygen to molecular Nitrogen ratio (O/N₂), large number of storms and other possible factors such as variations in storm’s intensity and local time dependence of the storm onset is expected to validate the observations in this study.     

Life under solar UV radiation in aquatic organisms.

Aquatic photosynthetic organisms are exposed to solar ultraviolet (UV) radiation while they harvest longer wavelength radiation for energetic reasons. Solar UV-B radiation (280-315 nm) affects motility and orientation in motile organisms and impairs photosynthesis in cyanobacteria, phytoplankton and macroalgae as measured by monitoring oxygen production or pulse amplitude modulated fluorescence analysis. Upon moderate UV stress most organisms respond by photoinhibition which is an active downregulation of the photosynthetic electron transport in photosystem II by degradation of UV-damaged D1 protein. Photoinhibition is readily reversible during recovery in shaded conditions. Excessive UV stress causes photodamage which is not easily reversible. Another major target is the DNA where UV-B mainly induces thymine dimers. Cyanobacteria, phytoplankton and macroalgae produce scytonemin, mycosporine-like amino acids and other UV-absorbing substances to protect themselves from short wavelength solar radiation.     

The ion energy spectra in the ring current during the geomagnetic storm of February, 1986

Using data from the CHEM instrument on the AMPTE/CCE spacecraft, we follow the development of the ring current energy spectra (1–300 keV/e) of the ion species H⁺, O⁺, He⁺, and He⁺⁺ in the post-noon and pre-noon local time sectors during the geomagnetic storm of February 1986. By comparing displays of phase space density, f, vs. magnetic moment, μ, we can distinguish between enhancements due to newly injected ions and those due to adiabatic energization of a pre-existing population. In both the local time sectors, the initial drop in Dst is associated with enhanced phase space densities of all species. The spectra observed during the pass when the Dst dropped to a minimem of −312 nT show a strong local time asymmetry. In the post-noon sector, the spectra showed the influx of a new population of ions, rich in O⁺ and He⁺⁺. In the pre-noon sector, the flux increase was consistent with adiabatic energization of the ion population injected earlier in the storm. This local time difference is consistent with a greatly enhanced convection electric field which brings a new population from the magnetotail to the post-noon, but not the pre-noon local time sector.