Biological potential of Martian hydrothermal systems

A source of energy to power metabolism may be a limiting factor in the abundance and spatial distribution of past or extant life on Mars. Although a global average of chemical energy available for microbial metabolism and biomass production on Mars has been estimated previously, issues of how the energy is distributed and which particular environments have the greatest potential to support life remain unresolved. We address these issues using geochemical models to evaluate the amounts of chemical energy available in one potential biological environment, Martian hydrothermal systems. In these models, host rock compositions are based upon the compositions of Martian meteorites, which are reacted at high temperature with one of three groundwater compositions. For each model, the values for Gibbs energy of reactions that are important for terrestrial chemosynthetic organisms and likely representative for putative Martian microbes are calculated. Our results indicate that substantial amounts of chemical energy may be available in these systems, depending most sensitively upon the composition of the host rock. From the standpoint of sources of metabolic energy, it is likely that suitable environments exist to support Martian life.     

Geochemical constraints on sources of metabolic energy for chemolithoautotrophy in ultramafic-hosted deep-sea hydrothermal systems

Numerical models are employed to investigate sources of chemical energy for autotrophic microbial metabolism that develop during mixing of oxidized seawater with strongly reduced fluids discharged from ultramafic-hosted hydrothermal systems on the seafloor. Hydrothermal fluids in these systems are highly enriched in H(2) and CH(4) as a result of alteration of ultramafic rocks (serpentinization) in the subsurface. Based on the availability of chemical energy sources, inferences are made about the likely metabolic diversity, relative abundance, and spatial distribution of microorganisms within ultramafic-hosted systems. Metabolic reactions involving H(2) and CH(4), particularly hydrogen oxidation, methanotrophy, sulfate reduction, and methanogenesis, represent the predominant sources of chemical energy during fluid mixing. Owing to chemical gradients that develop from fluid mixing, aerobic metabolisms are likely to predominate in low-temperature environments (<20-30 degrees C), while anaerobes will dominate higher-temperature environments. Overall, aerobic metabolic reactions can supply up to approximately 7 kJ of energy per kilogram of hydrothermal fluid, while anaerobic metabolic reactions can supply about 1 kJ, which is sufficient to support a maximum of approximately 120 mg (dry weight) of primary biomass production by aerobic organisms and approximately 20-30 mg biomass by anaerobes. The results indicate that ultramafic-hosted systems are capable of supplying about twice as much chemical energy as analogous deep-sea hydrothermal systems hosted in basaltic rocks.     

Formate as an energy source for microbial metabolism in chemosynthetic zones of hydrothermal ecosystems

Formate, a simple organic acid known to support chemotrophic hyperthermophiles, is found in hot springs of varying temperature and pH. However, it is not yet known how metabolic strategies that use formate could contribute to primary productivity in hydrothermal ecosystems. In an effort to provide a quantitative framework for assessing the role of formate metabolism, concentration data for dissolved formate and many other solutes in samples from Yellowstone hot springs were used, together with data for coexisting gas compositions, to evaluate the overall Gibbs energy for many reactions involving formate oxidation or reduction. The result is the first rigorous thermodynamic assessment of reactions involving formate oxidation to bicarbonate and reduction to methane coupled with various forms of iron, nitrogen, sulfur, hydrogen, and oxygen for hydrothermal ecosystems. We conclude that there are a limited number of reactions that can yield energy through formate reduction, in contrast to numerous formate oxidation reactions that can yield abundant energy for chemosynthetic microorganisms. Because the energy yields are so high, these results challenge the notion that hydrogen is the primary energy source of chemosynthetic microbes in hydrothermal ecosystems.     

The potential for lithoautotrophic life on Mars: application to shallow interfacial water environments

We developed a numerical model to assess the lithoautotrophic habitability of Mars based on metabolic energy, nutrients, water availability, and temperature. Available metabolic energy and nutrient sources were based on a laboratory-produced Mars-analog inorganic chemistry. For this specific reference chemistry, the most efficient lithoautotrophic microorganisms would use Fe(2+) as a primary metabolic electron donor and NO(3)(-) or gaseous O(2) as a terminal electron acceptor. In a closed model system, biomass production was limited by the electron donor Fe(2+) and metabolically required P, and typically amounted to approximately 800 pg of dry biomass/ml ( approximately 8,500 cells/ml). Continued growth requires propagation of microbes to new fecund environments, delivery of fresh pore fluid, or continued reaction with the host material. Within the shallow cryosphere--where oxygen can be accessed by microbes and microbes can be accessed by exploration-lithoautotrophs can function within as little as three monolayers of interfacial water formed either by adsorption from the atmosphere or in regions of ice stability where temperatures are within some tens of degrees of the ice melting point. For the selected reference host material (shergottite analog) and associated inorganic fluid chemistry, complete local reaction of the host material potentially yields a time-integrated biomass of approximately 0.1 mg of dry biomass/g of host material ( approximately 10(9) cells/g). Biomass could also be sustained where solutes can be delivered by advection (cryosuction) or diffusion in interfacial water; however, both of these processes are relatively inefficient. Lithoautotrophs in near-surface thin films of water, therefore, would optimize their metabolism by deriving energy and nutrients locally. Although the selected chemistry and associated model output indicate that lithoautotrophic microbial biomass could accrue within shallow interfacial water on Mars, it is likely that these organisms would spend long periods in maintenance or survival modes, with instantaneous biomass comparable to or less than that observed in extreme environments on Earth.     

Comparative Metagenomics of Two Microbial Mats at Cuatro Ciénegas Basin II: Community Structure and Composition in Oligotrophic Environments

Abstract Microbial mats are self-sustained, functionally complex ecosystems that make good models for the understanding of past and present microbial ecosystems as well as putative extraterrestrial ecosystems. Ecological theory suggests that the composition of these communities might be affected by nutrient availability and disturbance frequency. We characterized two microbial mats from two contrasting environments in the oligotrophic Cuatro Ciénegas Basin: a permanent green pool and a red desiccation pond. We analyzed their taxonomic structure and composition by means of 16S rRNA clone libraries and metagenomics and inferred their metabolic role by the analysis of functional traits in the most abundant organisms. Both mats showed a high diversity with metabolically diverse members and strongly differed in structure and composition. The green mat had a higher species richness and evenness than the red mat, which was dominated by a lineage of Pseudomonas. Autotrophs were abundant in the green mat, and heterotrophs were abundant in the red mat. When comparing with other mats and stromatolites, we found that taxonomic composition was not shared at species level but at order level, which suggests environmental filtering for phylogenetically conserved functional traits with random selection of particular organisms. The highest diversity and composition similarity was observed among systems from stable environments, which suggests that disturbance regimes might affect diversity more strongly than nutrient availability, since oligotrophy does not appear to prevent the establishment of complex and diverse microbial mat communities. These results are discussed in light of the search for extraterrestrial life. Key Words: Cuatro Ciénegas-Metagenomics-Microbial mats-Oligotrophic-Phosphorus limitation-Stromatolites. Astrobiology 12, 659-673.     

Radiolytic hydrogen and microbial respiration in subsurface sediments

Radiolysis of water may provide a continuous flux of an electron donor (molecular hydrogen) to subsurface microbial communities. We assessed the significance of this process in anoxic marine sediments by comparing calculated radiolytic H(2) production rates to estimates of net (organic-fueled) respiration at several Ocean Drilling Program (ODP) Leg 201 sites. Radiolytic H(2) yield calculations are based on abundances of radioactive elements (uranium, thorium, and potassium), porosity, grain density, and a model of water radiolysis. Net respiration estimates are based on fluxes of dissolved electron acceptors and their products. Comparison of radiolytic H(2) yields and respiration at multiple sites suggests that radiolysis gains importance as an electron donor source as net respiration and organic carbon content decrease. Our results suggest that radiolytic production of H(2) may fuel 10% of the metabolic respiration at the Leg 201 site where organic-fueled respiration is lowest (ODP Site 1231). In sediments with even lower rates of organic-fueled respiration, water radiolysis may be the principal source of electron donors. Marine sedimentary ecosystems may be useful models for non-photosynthetic ecosystems on early Earth and on other planets and moons, such as Mars and Europa.     

太阳能飞行器能源昼夜闭环仿真分析

以能量为核心，建立太阳能飞行器的获能模型；通过对太阳能飞行器飞行剖面特点的分析，设计飞行过程中各个阶段的飞行方案，并建立相应的耗能模型；考虑目前储能电池技术水平，根据产能和耗能模型，建立储能模型，完成了能源闭环模型的设计；参照Zephyr 7太阳能无人机的结构参数对论文建立的模型进行了仿真分析，获取了飞行过程中能量变化规律，同时对飞行姿态进行优化。结果表明：通过对太阳能飞行器昼夜飞行高度的不同设置以及飞行姿态角的优化，太阳能飞行器可以实现跨昼夜持久飞行。     

An energy balance concept for habitability

Habitability can be formulated as a balance between the biological demand for energy and the corresponding potential for meeting that demand by transduction of energy from the environment into biological process. The biological demand for energy is manifest in two requirements, analogous to the voltage and power requirements of an electrical device, which must both be met if life is to be supported. These requirements exhibit discrete (non-zero) minima whose magnitude is set by the biochemistry in question, and they are increased in quantifiable fashion by (i) deviations from biochemically optimal physical and chemical conditions and (ii) energy-expending solutions to problems of resource limitation. The possible rate of energy transduction is constrained by (i) the availability of usable free energy sources in the environment, (ii) limitations on transport of those sources into the cell, (iii) upper limits on the rate at which energy can be stored, transported, and subsequently liberated by biochemical mechanisms (e.g., enzyme saturation effects), and (iv) upper limits imposed by an inability to use "power" and "voltage" at levels that cause material breakdown. A system is habitable when the realized rate of energy transduction equals or exceeds the biological demand for energy. For systems in which water availability is considered a key aspect of habitability (e.g., Mars), the energy balance construct imposes additional, quantitative constraints that may help to prioritize targets in search-for-life missions. Because the biological need for energy is universal, the energy balance construct also helps to constrain habitability in systems (e.g., those envisioned to use solvents other than water) for which little constraint currently exists.     

Experimental modeling of impact of space dust and debris on flying vehicles and their components

For modeling the space dust and debris effect on flying vehicles, an investigation of the low-velocity impact of corundum and tungsten powders, accelerated by explosion, with particle size up to 50 microns on steel and duralumin targets was carried out. Also studied was the impact of sewing needles against metal and dielectric barriers, antimeteor shield models, and duralumin containers with hard materials, gunpowder, and explosives. At impact of powders at velocities of up to 2 km/s and needles at a velocity of up to 0.5 km/s against metals, the channels arose with lengths greater than 100 and 50 diameters of a striker. At impact of needles, the containers with hard explosive materials were destroyed because of ignition of their contents, and containers with plastic explosive were punched through, and no burning occurred. The energy, released at destruction of plexiglas blocks and containers with hard materials, many times exceeded the impact energy due to release of the elastic energy stored in them.     

The possible origin and persistence of life on Enceladus and detection of biomarkers in the plume

The jets of icy particles and water vapor issuing from the south pole of Enceladus are evidence for activity driven by some geophysical energy source. The vapor has also been shown to contain simple organic compounds, and the south polar terrain is bathed in excess heat coming from below. The source of the ice and vapor, and the mechanisms that accelerate the material into space, remain obscure. However, it is possible that a liquid water environment exists beneath the south polar cap, which may be conducive to life. Several theories for the origin of life on Earth would apply to Enceladus. These are (1) origin in an organic-rich mixture, (2) origin in the redox gradient of a submarine vent, and (3) panspermia. There are three microbial ecosystems on Earth that do not rely on sunlight, oxygen, or organics produced at the surface and, thus, provide analogues for possible ecologies on Enceladus. Two of these ecosystems are found deep in volcanic rock, and the primary productivity is based on the consumption by methanogens of hydrogen produced by rock reactions with water. The third ecosystem is found deep below the surface in South Africa and is based on sulfur-reducing bacteria consuming hydrogen and sulfate, both of which are ultimately produced by radioactive decay. Methane has been detected in the plume of Enceladus and may be biological in origin. An indicator of biological origin may be the ratio of non-methane hydrocarbons to methane, which is very low (0.001) for biological sources but is higher (0.1-0.01) for nonbiological sources. Thus, Cassini's instruments may detect plausible evidence for life by analysis of hydrocarbons in the plume during close encounters.     

Space light: space industrial enhancement of the solar option

The solar option can be enhanced significantly by space light technology. Reflectors in suitable orbits beam to Earth measured amounts of sunlight, the most versatile and bio-compatible energy source. The multitude of space light functions ranges from night illumination of rural and urban areas (by Lunetta systems) to photosynthetic production enhancement for the growth of food and of biomass for conversion to chemical fuels, local agricultural irradiation for crop drying and weather stabilization and to electric power generation by irradiating suitable photovoltaic or thermal ground receivers at night or by adding to the natural solar energy input during daytime (Soletta systems).

The Lunetta and Soletta concepts, developed by the author during the past ten years, building on the foundations laid by the great space pioneer Prof. H. Oberth (1928), are reviewed, along with their socio-economic merits. An assessment of terrestrial alternatives shows that many useful functions have no practical alternative, the major exception being electric power generation. Three systems are selected, bracketing the broad versatility of space light—Lunetta, Powersoletta and a large Biosoletta for large-scale seafood production in Antarctic and Artic waters. The systems, and several maintenance and supply requirements are described, sized and analyzed, along with suitable orbit selection for different applications. Models are developed for rural and urban area lighting, power generation at selected sites around the globe with photovoltaic and thermal ground stations and for the large-scale production of seafood at high southern and northern latitudes with ample nutrient upwell, but insufficient annual supply of solar energy. The economics of these systems is analyzed.     

高层大气模型对空间站轨道漂移和寿命的影响分析

本文以轨道摄动分析方法一阶理论为基础，其中大气阻力摄动采用数值积分方法，给出一种可利用各种大气模型进行轨道摄动分析的计算方法，并利用三种高层大气模型（ＣＩＲＡ—７２，ＣＩＲＡ—８６和ＤＴＭ）和三个太阳活动水平（Ｆ１０．７＝１００，１５０和２００）分析比较了大气阻力振动对高度为４００ｋｍ的空间站轨道漂移和寿命的影响，以及估算修正轨道漂移所需的能量。给出的定量分析结果将为空间站或航天飞行器的轨道设计和能量估算提供依据。     

A combinatorial approach to biochemical space: description and application to the redox distribution of metabolism

Redox chemistry is central to life on Earth. It is well known that life uses redox chemistry to capture energy from environmental chemical energy gradients. Here, we propose that a second use of redox chemistry, related to building biomass from environmental carbon, is equally important to life. We apply a method based on chemical structure to evaluate the redox range of different groups of terrestrial biochemicals, and find that they are consistently of intermediate redox range. We hypothesize the common intermediate range is related to the chemical space required for the selection of a consistent set of metabolites. We apply a computational method to show that the redox range of the chemical space shows the same restricted redox range as the biochemicals that are selected from that space. By contrast, the carbon from which life is composed is available in the environment only as fully oxidized or reduced species. We therefore argue that redox chemistry is essential to life for assembling biochemicals for biomass building. This biomass-building reason for life to require redox chemistry is in addition (and in contrast) to life's use of redox chemistry to capture energy. Life's use of redox chemistry for biomass capture will generate chemical by-products-that is, biosignature gases-that are not in redox equilibrium with life's environment. These potential biosignature gases may differ from energy-capture redox biosignatures.     

低轨航天器运行环境质子通量预测模型对比研究

辐射带粒子环境是导致航天器故障和异常的重要因素。为此,需要对辐射带粒子环境及其通量分布进行研究。文章主要研究低地球轨道（LEO）环境辐射带质子分布情况。分析了目前用来计算质子通量的几种常用模型的优缺点;利用各模型计算了不同轨道的质子通量,对计算结果进行了比较;总结了进行不同高度、不同能量范围的质子通量计算时,选择不同模型的依据原则。     

Planetary resources and astroecology. Planetary microcosm models of asteroid and meteorite interiors: electrolyte solutions and microbial growth--implications for space populations and panspermia

Planetary microcosms were constructed using extracts from meteorites that simulate solutions in the pores of carbonaceous chondrites. The microcosms were found to support the growth of complex algal and microbial populations. Such astroecology experiments demonstrate how a diverse ecosystem could exist in fluids within asteroids, and in meteorites that land on aqueous planets. The microcosm solutions were obtained by extracting nutrient electrolytes under natural conditions from powders of the Allende (CV3) and Murchison (CM2) meteorites at low (0.02 g/ml) and high (10.0 g/ml) solid/solution ratios. The latter solutions contain > 3 mol/L electrolytes and about 10 g/L organics, that simulate natural fluids in asteroids during aqueous alteration and in the pores of meteorites, which can help prebiotic synthesis and the survival of early microorganisms. These solutions and wet solids were in fact found to support complex self-sustaining microbial communities with populations of 4 x 10(5) algae and 6 x 10(6) bacteria and fungi for long periods (> 8 months). The results show that planetary microcosms based on meteorites can: assay the fertilities of planetary materials; identify space bioresources; target astrobiology exploration; and model past and future space-based ecosystems. The results show that bioresources in the carbonaceous asteroids can sustain a biomass of 10(18) kg, comprising 10(32) microorganisms and a human population of 10(14). The results also suggest that protoplanetary nebulae can support and disperse microorganisms and can be therefore effective environments for natural and directed panspermia.     

Earth weather and climate control: can space technology contribute?

With ongoing progress in space technology, questions of its potential for the modification of weather and climate phenomena (often summarized by the term ‘geoengineering’) ranging from small-scale severe weather events to mitigation of effects caused by global climate change and ozone depletion have become popular. This paper reviews the current state of scientifically based studies in this context and attempts to provide a basis for an assessment of geoengineering efforts with respect to technological, economic and fundamental scientific aspects. The overview indicates that the current state of knowledge about climate variability as a consequence of natural and anthropogenic influences is sufficient to classify geoengineering solutions as highly risky and their consequences as extremely difficult to predict. Even on smaller scales and with less complexity of interacting processes, only very limited boundary conditions, i.e. a narrow range of atmospheric variability and land surface topography favouring the intended alteration, seem to justify weather modification. Moreover, as for systems reaching scales of large organized storms and hurricanes, required energy and control resources are well beyond existing capabilities. Consequently, the use of space technology for provision of better information on environmental change and integration of remote sensing data into weather and climate models forecasts is supported.     

The biological terraforming of Mars: planetary ecosynthesis as ecological succession on a global scale

Mars is bitterly cold and dry, but robotic spacecraft have returned abundant data that indicate Mars once had a much warmer and wetter climate in the past. These data, the basis of the search for past or present life on Mars, suggest the possibility of returning Mars to its previous climate by global engineering techniques. Greenhouse gases, such as perfluorocarbons, appear to be the best method for warming Mars and increasing its atmospheric density so that liquid water becomes stable. The process of making Mars habitable for terrestrial organisms is called terraforming or planetary ecosynthesis. The process of introducing terrestrial ecosystems to Mars can be compared with a descent down a high mountain. Each drop in elevation results in a warmer, wetter climate and more diverse biological community. Beginning with a polar desert, the sequence of ecosystems passes through tundra, boreal forest, and temperate ecosystems where moisture determines the presence of desert, grassland, or forest. This model suggests a sequence for the introduction of ecosystems to Mars and the communities to search for potential colonizing species for Mars.     

Biomarker and stable isotope characterization of coastal pond-derived organic matter, McMurdo Dry Valleys, Antarctica

Small coastal ponds that contain photosynthetic microbial mat communities represent an extreme environment where a potentially significant source of labile organic carbon can be found within the McMurdo Dry Valleys, Antarctica. To distinguish coastal pond-derived organic matter from other sources of organic matter in the Dry Valleys, bulk organic carbon, nitrogen, and sulfur isotope signatures and phospholipid fatty acid (PLFA) profiles of benthic microbial mats located at two sites--Hjorth Hill coast and Garwood Valley--were investigated. The average isotope values at Hjorth Hill coast and Garwood Valley are, respectively, -10.9 per thousand and -10.2 per thousand for delta(13) C, 3.7 per thousand and -1.3 per thousand for delta(15)N, and 8.1 per thousand and 16.7 per thousand for delta(34)S. Microbial mats from all ponds are dominated by monounsaturated PLFAs (indicative of Gram-negative bacteria) and polyunsaturated PLFAs (indicative of microeukaryotes). Biomarkers specific to aerobic prokaryotes, eukaryotes, and photoautotrophic microeukaryotes, as well as sulfur-reducing bacteria, are present in all samples. Benthic mats at Garwood Valley are thicker and more laminated, have a higher biomass, and have a greater carbon and nitrogen content, which suggests greater productivity than mats at Hjorth Hill coast. Greater productivity is supported, as well, by higher dissolved oxygen contents likely derived from heightened photosynthetic productivity. More productivity at Garwood Valley likely results from a larger influx of terrestrial surface waters together with a concomitant nutrient loading.     

Study of radiation conditions onboard the International space station by means of the Liulin-5 dosimeter

For estimating radiation risk in space flights it is necessary to determine radiation dose obtained by critical organs of a human body. For this purpose the experiments with human body models are carried out onboard spacecraft. These models represent phantoms equipped with passive and active radiation detectors which measure dose distributions at places of location of critical organs. The dosimetric Liulin-5 telescope is manufactured with using three silicon detectors for studying radiation conditions in the spherical tissue-equivalent phantom on the Russian segment of the International space station (ISS). The purpose of the experiment with Liulin-5 instrument is to study dynamics of the dose rate and particle flux in the phantom, as well as variations of radiation conditions on the ISS over long time intervals depending on a phase of the solar activity cycle, orbital parameters, and presence of solar energetic particles. The Liulin-5 dosimeter measures simultaneously the dose rate and fluxes of charged particles at three depths in the radial channel of the phantom, as well as the linear energy transfer. The paper presents the results of measurements of dose rate and particle fluxes caused by various radiation field components on the ISS during the period from June 2007 till December 2009.     

卫星能源约束检查模型改进及仿真

卫星能源约束检查模型对卫星任务规划有重要参考作用,可防止因能源不足而导致卫星故障或任务不能顺利完成。现有的能源约束检查模型存在部分问题:人为设定计算初始值,导致计算不准确;能源约束检查不通过时未给出符合能源约束的任务调整建议,导致任务不能快速调整;未对计算误差进行校正,导致误差积累。对此,提出了一种改进的能源约束检查模型,在原有模型中加入能源数据库对卫星运行的能源数据进行记录,从而准确获取初始值,并在模型中加入任务调整和遥测校正功能。该模型可实现能源约束计算自动管理及任务自动调整功能,缩短任务规划周期,提升紧急任务的执行效率。对改进的模型进行仿真,结果表明:模型预测结果与预期值相符,任务调整和能源校正功能实现良好,能实际应用到运控系统中进行规划任务能源约束检查和能源管理。