Long-term preservation of microbial ecosystems in permafrost.

It has been established that significant numbers (up to 10 million cells per gram of sample) of living microorganisms of various ecological and morphological groups have been preserved under permafrost conditions, at temperatures ranging from -9 to -13 degrees C and depths of up to 100 m, for thousands and sometimes millions of years. Preserved since the formation of permafrost in sand-clay sediments of the Pliocene-Quaternary period and in paleosols and peats buried among them, these cells art the only living organisms that have survived for a geologically significant period of time. The complexity of the microbial community preserved varies with the age of the permafrost. Eukaryotes are found only in Holocene sediments; while prokaryotes are found to greater ages, i.e., Pliocene and Pleistocene. The diversity of microorganisms decreases with increasing age of sediments, and as a result cocci and corynebacteria are predominant. Enzyme activity (catalase and hydrolytic enzymes) and photosynthetic pigments (chlorophyll and pheophytin have also been detected in permafrost sediments. These results permit us to outline some approaches to the search for traces of life in the permafrost of Martian sediments by borehole core sampling. It is in the deep horizons (and not on the planet surface), isolated by permafrost from the external conditions, that results similar to those obtained on Earth can be expected.     

Preservation of cell structures in permafrost: a model for exobiology.

The present report is the first contribution toward a comprehensive fine-structural study of microbial cells from permafrost. Prokaryotes with a variety of cell wall types demonstrate high stability of cell structure after long-term cryopreservation in frozen soils and sediments of the Arctic. The surface capsular layers that were a salient feature of the cells both in situ and on nutrient media may be an adaptation to low temperature. To the extent that permafrost regions on Earth approximate Martian conditions, preservation of cell structure there can serve as the basis for predictions about preservation in Martian permafrost sediments.     

低剂量电离辐射环境下微生物群落物种多样性的产生和维持机制

空间站微生物严重滋生现象表明：在低剂量电离辐射（Low-Dose Ionizing Radiation,LDIR）环境下,微生物群落物种多样性水平大大增加,其机制目前尚不清楚。在前期调查和具体实验观测的基础上,本研究首次提出LDIR能够使微生物群落中的物种产生不同程度的生长延迟效应,从而减小了物种之间的竞争排斥,诱导并维持了微生物群落的物种多样性。本研究基于经典的Lotka-Volterra竞争模型,将生长延迟时间环节引入其中,得到LDIR下微生物群落的演替模型,在Matlab/Simulink平台上进行大规模的计算机仿真实验,获得LDIR下微生物多样性产生和维持的动力学机制,得到结果可为认识低剂量电离辐射环境下微生物群落的演替过程提供科学的理论依据。     

Satellite observed dynamics of chlorophyll and suspended sediments in a shallow,high latitude embayment

A study of the distribution of chlorophyll and suspended sediments in Norton Sound, Alaska, in May and July 1979 and 1980, was made using imagery from both raw and processed data collected by the Nimbus-7 CZCS. False color images generated from the raw data in bands 1,2, and 4 showed the large seasonal changes which take place in the north and easterly movement of suspended sediments from the Yukon River. The atmospherically corrected pigment images suggest that, except in open waters, the outflow from the Yukon and other local rivers strongly influences the nature of the planktonic environment in Norton Sound. Comparison of the patterns shown by the two different kinds of imagery sometimes reveals features which would otherwise have been masked in waters with such a significant sediment load. In general, the distribution and concentration of chlorophyll and suspended sediments in the imagery provided a useful means for determining the relative importance of winds, currents, ice, and river runoff in the biological and physical oceanography of the surface waters of Norton Sound.     

Peroxides and the survivability of microorganisms on the surface of Mars.

Results of the Viking mission seem to indicate that there is a ubiquitous layer of highly oxidizing aeolian material covering the Martian surface. This layer is thought to oxidize organic material that may settle on it, and is therefore responsible for the lack of detection of organic matter on the planet's surface by Viking. The mechanism that creates the oxidizing condition is not well understood, nor is the extent of the oxidation potential of this material. It has been suggested that the oxidizing nature of the soil is due to photochemical reactions which create hydrogen peroxide and superoxides in the surface soil. One question of importance to planetary protection regarding this material is, what is its potential for destroying terrestrial microorganisms, thus making the surface of Mars "self-sterilizing"? Using data obtained by the gas exchange experiment on Viking, and for simplicity assuming that all of the O2 released came from H2O2, the concentration range for H2O2 on the surface of Mars can be calculated to be 25-250 ppm. The microbial disinfection rate by H2O2 is concentration dependent, and is highly variable within the microbial community. Data from our laboratory indicate that certain soil bacteria survive and grow to stationary phase in 30,000 ppm H2O2. However, the total number of organisms decreases in the presence of H2O2. These results indicate that it is doubtful that the presence of H2O2 alone on Mars would make the surface "self-sterilizing".     

MEP (Mars Environment Package): toward a package for studying environmental conditions at the surface of Mars from future lander/rover missions.

In view to prepare Mars human exploration, it is necessary to promote and lead, at the international level, a highly interdisciplinary program, involving specialists of geochemistry, geophysics, atmospheric science, space weather, and biology. The goal of this program will be to elaborate concepts of individual instruments, then of integrated instrumental packages, able to collect exhaustive data sets of environmental parameters from future landers and rovers of Mars, and to favour the conditions of their implementation. Such a program is one of the most urgent need for preparing human exploration, in order to develop mitigation strategies aimed at ensuring the safety of human explorers, and minimizing risk for surface operations. A few main areas of investigation may be listed: particle and radiation environment, chemical composition of atmosphere, meteorology, chemical composition of dust, surface and subsurface material, water in the subsurface, physical properties of the soil, search for an hypothesized microbial activity, characterization of radio-electric properties of the Martian ionosphere. Scientists at the origin of the present paper, already involved at a high degree of responsibility in several Mars missions, and actively preparing in situ instrumentation for future landed platforms (Netlander--now cancelled, MSL-09), express their readiness to participate in both ESA/AURORA and NASA programs of Mars human exploration. They think that the formation of a Mars Environment working group at ESA, in the course of the AURORA definition phase, could act positively in favour of the program, by increasing its scientific cross-section and making it still more focused on human exploration.     

The structure and function of microbial communities in recirculating hydroponic systems.

Strategies to control the microbial community associated with plant growth systems need to be based on a fundamental understanding of the factors which structure and regulate the community. Spatial and temporal patterns in the abundance and production rate of microorganisms in hydroponic systems containing wheat were examined to evaluate how root-derived carbon is processed. The relevance of results to monitoring and control strategies is discussed.     

Density and composition of microorganisms during long-term (418 day) growth of potato using biologically reclaimed nutrients from inedible plant biomass.

A study evaluating alternative methods for long term operation of biomass production systems was recently completed at the Kennedy Space Center (KSC). The 418-day study evaluated repeated batch versus mixed-aged production of potato grown on either standard 1/2-strength Hoagland's nutrient solution or solutions including nutrients recycled from inedible plant material. The long term effects of closure and recycling on microbial dynamics were evaluated by monitoring the microbial communities associated with various habitats within the plant growth system (i.e., plant roots, nutrient solution, biofilms within the hydroponic systems, atmosphere, and atmospheric condensate). Plate count methods were used to enumerate and characterize microorganisms. Microscopic staining methods were used to estunate total cell densities. The primary finding was that the density and composition of microbial communities associated with controlled environmental plant growth systems are stable during long term operation. Continuous production resulted in slightly greater stability. Nutrient recycling, despite the addition of soluble organic material from the waste processing system, did not significantly increase microbial density in any of the habitats.     

The initiation of biological processes on Earth: summary of empirical evidence.

With the currently available geological record at band, the existence of life on this planet as from at least 3.8 Gyr ago seems so firmly established as to be virtually unassailable. Specifically, various disparate lines of evidence have merged to indicate (1) that the surface of the Archaean Earth had hosted prolific microbial ecosystems as is testified by a quasi-continuous record of microbialites ("stromatolites") and associated microfossils of prokaryotic affinity over 3.5, if not 3.8 Gyr of geological history, and (2) that the sedimentary carbon record has preserved the isotopic signature of autotrophic (notably photosynthetic) carbon fixation over the same time span. With the observed enrichment of isotopically light carbon in sedimentary organic matter largely consonant with the bias in favor of 12C during photosynthesis, the mainstream of the carbon isotope record can be best explained as geochemical manifestation of the isotope discriminating properties of the ribulose-1,5-bisphosphate (RuBP) carboxylase reaction of the Calvin cycle suggesting an extreme degree of evolutionary conservatism in the biochemistry of autotrophic carbon fixation. As a consequence, partial biological control of the geochemical carbon cycle was established already during Early Archaean times and fully operative by the time of formation of the Earth's earliest sediments.     

Surface shape control of integrated paraboloid space reflector consisting of sub-reflectors exploiting modulated solar pressure

Reflectors are very critical space elements and can be used not only as solar collectors/reflectors, telecommunication radio antennas and telescopes but also for dual-usage such as solar sails and solar concentrators to probe and sublimate materials from asteroids when actively controlling the surface shape. In this paper, the surface shape of a slack reflector with negligible elastic deformations will be controlled to be a paraboloid by actively modulating the solar radiation pressure (SRP) force using reflectivity control devices (RCDs) across the reflector. Nonlinear static equilibrium equations for an arbitrary infinitesimal within the reflector along the radial, circumferential and transverse directions are established considering the external modulated SRP force and internal tensions respectively. The coupled radial stress differential governing equation and reflectivity algebraic equation are obtained for the paraboloid reflector by the help of the formulation of an inverse problem based on equilibrium equations previously established. Some analytical and numerical analysis for reflectors with ideal and non-perfect SRP force models are performed respectively. The conclusions concerning about how to control the reflector’s surface shape successfully using allowed reflectivity, resulting in reasonable stress range, moreover, how to get the feasible solutions influenced by the reflector’s size parameters, are all based on the presented analytical and numerical analysis.     

航天器AIT中心微生物多样性分析

航天器AIT（总装、集成和测试）中心的洁净度控制是防止造成航天器正向污染的重要保障.利用高通量测序技术与传统培养法比较分析了中国某航天器AIT中心的空气微生物组成与多样性.基于高通量的测序分析结果显示:该AIT中心空气中优势细菌以芽孢杆菌属为主,相对丰度78.47%±1.59%;优势真菌为银耳目,相对丰度8.97%±0.93%;基于培养法获得的优势细菌为葡萄球菌属,且未获得真菌培养物.空气微生物的chao1指数、Simpson多样性指数以及Shannon多样性指数分析显示,该AIT中心空气中细菌的多样性水平均高于真菌.      

METHIS: Mercury thermal infrared spectrometer

The BepiColombo mission to Mercury is devoted to the thorough exploration of Mercury and its environment, with the aim to understand the processes of planetary formation and evolution in the hottest part of the protoplanetary nebula. This mission represents an unique opportunity for the European community to extend the understanding of the Solar Nebula evolution from its outer edge – ideally represented by comets – to its inner and warmer edge. Obviously this exploration asks for a detailed knowledge of the main constituents of the matter present in the different Solar System areas. Spectroscopy is a powerful tool to acquire this knowledge. We have participated with a large consortium of European researchers to the development of the Rosetta imaging spectrometer. We propose here to use our experience to develop a newly designed spectrometer to investigate the mineralogical composition of the Mercurial surface. Given the particular thermodynamical situation of the Mercurial surface, we have developed a concept that combines a medium IR low spectral resolution imager with a moderate spectral resolution NIR point spectrometer. The main goal of METHIS is to provide the mineralogical characterisation of the surface with sufficient spectral resolution in a scientifically diagnostic spectral range.     

Inhibition of denitrification by ultraviolet radiation.

It has been shown that UV-A (lambda=320-400 nm) and UV-B (lambda=280-320 nm) inhibit photosynthesis, nitrogen fixation and nitrification. The purpose of this study was to determine the effects, if any, on denitrification in a microbial community inhabiting the intertidal. The community studied is the microbial mat consisting primarily of Lyngbya that inhabits the Pacific marine intertidal, Baja California, Mexico. Rates of denitrification were determined using the acetylene blockage technique. Pseudomonas fluorescens (ATCC #17400) was used as a control organism, and treated similarly to the mat samples. Samples were incubated either beneath a PAR transparent, UV opaque screen (OP3), or a mylar screen to block UV-B, or a UV transparent screen (UVT) for 2 to 3 hours. Sets of samples were also treated with nitrapyrin to inhibit nitrification, or DCMU to inhibit photosynthesis and treated similarly. Denitrification rates were greater in the UV protected samples than in the UV exposed samples the mat samples as well as for the Ps fluorescens cultures. Killed controls exhibited no activity. In the DCMU and nitrapyrin treated samples denitrification rates were the same as in the untreated samples. These data indicate that denitrification is directly inhibited by UV radiation.     

Analysis of the archaeal sub-seafloor community at Suiyo Seamount on the Izu-Bonin Arc

A sub-surface archaeal community at the Suiyo Seamount in the Western Pacific Ocean was investigated by 16S rRNA gene sequence and whole-cell in situ hybridization analyses. In this study, we drilled and cased holes at the hydrothermal area of the seamount to minimize contamination of the hydrothermal fluid in the sub-seafloor by penetrating seawater. PCR clone analysis of the hydrothermal fluid samples collected from a cased hole indicated the presence of chemolithoautotrophic primary biomass producers of Archaeoglobales and the Methanococcales-related archaeal HTE1 group, both of which can utilize hydrogen as an electron donor. We discuss the implication of the microbial community on the early history of life and on the search for extraterrestrial life.     

Models of P/Borrelly: Activity and dust mantle formation

Comet 19P/Borrelly was observed by Deep Space One spacecraft on September 22, 2001 (Soderblom et al., 2002).The DS1 images show a very dark and elongate nucleus with a complex topography; the IR spectra show a strong red-ward slope consistent with a very hot and dry surface (345K to 300K). During DS1 encounter the comet coma was dominated by a prominent jet but most of the comet was inactive, confirming the Earth-based observations that <10% of the surface is actively sublimating. We have developed a thermal evolution model of comet PBorrelly, using a numerical code that is able to solve the heat conduction and gas diffusion equations at the same time across an idealized spherical nucleus ( De Sanctis et al., 1999, 2000; Capria et al., 2000; Coradini et al., 1997a,b). The comet nucleus is composed by water, volatiles ices and dust in different proportions. The refractory component is made by grains that are embedded in the icy matrix. The code is able to account for the dust release, contributing to the dust flux, and the formation of dust mantles on the comet surface. The model was applied to a cometary nucleus with the estimated physical and dynamical characteristics of P/Borrelly in order to infer the status and activity level of a body on such an orbit during the DS1 observation. The comet gas flux, differentiation and thermal behavior were simulated and reproduced. The model results are in good agreement with the DS1 flyby results and the ground based observations, in terms of activity, dust coverage and temperatures of the surface.     

Life on Mars: how it disappeared (if it was ever there).

The cryptoendolithic microbial community in the Ross Desert (McMurdo Dry Valleys) of Antarctica exists at temperatures significantly below the temperature optima of the primary producers. Surviving near the limit of their physiological adaptability, the organisms are under severe environmental stress, so further deterioration in the environment results in cell damage and death. The sequence of events leading to extinction is considered to be a terrestrial analog for disappearance of possible life on early Mars. Progressive stages of cell damage and death in the Ross Desert material are documented with transmission electron microscopy.     

Population dynamics of transgenic strain Escherichia coli Z905/pPHL7 in freshwater and saline lake water microcosms with differing microbial community structures.

Populations of Escherichia coli Z905/pPHL7, a transgenic microorganism, were heterogenic in the expression of plasmid genes when adapting to the conditions of water microcosms of various mineralization levels and structure of microbial community. This TM has formed two subpopulations (ampicillin-resistant and ampicillin-sensitive) in every microcosm. Irrespective of mineralization level of a microcosm, when E. coli Z905/pPHL7 alone was introduced, the ampicillin-resistant subpopulation prevailed, while introduction of the TM together with indigenous bacteria led to the dominance of the ampicillin-sensitive subpopulation. A high level of lux gene expression maintained longer in the freshwater microcosms than in sterile saline lake water microcosms. A horizontal gene transfer has been revealed between the jointly introduced TM and Micrococcus sp. 9/pSH1 in microcosms with the Lake Shira sterile water.     

Numerical simulations of the radiance from the comet 46P/Wirtanen in the various configurations of the measurements during “Rosetta” mission

The work we present deals with the spectrometric measurements of VIRTIS instrument of the Comet P/Wirtanen planned for the Rosetta mission. This spectrometer can monitor (VIRTIS M channel: 0.250μm – 0.980μm; Δκ=20cm⁻¹; 0.980 – 5.0 μm; Δκ = 5cm⁻¹; VIRTIS H channel: 2.0 μm – 5.0 μm; Δκ=2cm⁻¹) the nucleus and the coma in order to provide a general picture of coma's composition, the production of gas and dust, the relationship of coma production to surface composition and the structure and variation of mineralogy of the nucleus surface. During the mission the observation conditions of the spectroscopic investigation change due to different relative positions spacecraft/comet, and to the different illumination conditions of the surface at various distances of the comet to the Sun. The nucleus surface is continuously modified by the ice sublimation accompanied by gas and dust emission. Consequently the surface also its spectrophotometric properties changes and their monitoring can give a new insight. The important role of simulations is to predict the results of measurements in various experimental condition what, in the future, can help in interpretation of the measured data.

In this paper the first results of our simulation the radiance from the comet in the 0.25–5.0μm spectral range at two distances from the Sun (1AU and 3AU) are shown. The distance between the Rosetta orbiter and the nucleus surface as well as the sun zenith angles are taken into account according to the Rosetta mission phases. In fact the surface and coma properties vary along the comet orbit, and should be taken into account in our calculations. The optical parameters of the dust on the surface (e.g. reflectance) and in the coma (e.g. Q_ext) were calculated from optical constants of possible comet analogues. The thermodynamic parameters of the comet are taken from the models of comet evolution. Through this kind of modelling it is possible to identify the surface characteristics in spectra of the radiation from the surface of nucleus transmitted through the coma loaded with dust and gases.

Even if the “Rosetta mission” is postponed, with the consequence of a target change, we think that our idea and the method used for the simulations can be useful also for the new Rosetta target - the comet 67P/Churyumov Gerasimenko.     

表面等离子体共振生物传感器在微生物检测中的应用

在地球环境及航天空间环境中存在有多种多样的微生物,快速、准确地检测这些微生物成为亟待解决的问题.在众多微生物检测方法中,表面等离子体共振生物传感器凭借其快速、灵敏的检测特点,近年来发展迅速.本文综述了表面等离子体共振生物传感器在微生物检测领域中的应用,以期为地面及航天环境的微生物检测工作提供技术参考.