Stable Isotope Ratios as a Biomarker on Mars

As both Earth and Mars have had similar environmental conditions at least for some extended time early in their history (Jakosky and Phillips in Nature 412:237–244, 2001), the intriguing question arises whether life originated and evolved on Mars as it did on Earth (McKay and Stoker in Rev. Geophys. 27:189–214, 1989). Conceivably, early autotrophic life on Mars, like early life on Earth, used irreversible enzymatically enhanced metabolic processes that would have fractionated stable isotopes of the elements C, N, S, and Fe. Several important assumptions are made when such isotope fractionations are used as a biomarker. The purpose of this article is two-fold: (1) to discuss these assumptions for the case of carbon and to summarize new insights in abiologic reactions, and (2) to discuss the use of other stable isotope systems as a potential biomarker. It is concluded that isotopic biomarker studies on Mars will encounter several important obstacles. In the case of carbon isotopes, the most important obstacle is the absence of a contemporary abiologic carbon reservoir (such as carbonate deposits on Earth) to act as isotopic standard. The presence of a contemporary abiologic sulfate reservoir (evaporite deposits) suggests that sulfur isotopes can be used as a potential biomarker for sulfate-reducing bacteria. The best approach for tracing ancient life on Mars will be to combine several biomarker approaches; to search for complexity, and to combine small-scale isotopic variations with chemical, mineralogical, and morphological observations. An example of such a study can be a layer-specific correlation between δ ¹³C and δ ³⁴S within an ancient Martian evaporite, which morphologically resembles the typical setting of a shallow marine microbial mat.     

The Importance of Water for Life

Liquid water is essential for life as we know it, i.e. carbon-based life. Although other compound-solvent pairs that could exist in very specific physical environments could be envisaged, the elements essential to carbon and water-based life are among the most common in the universe. Carbon molecules and liquid water have physical and chemical properties that make them optimised compound-solvent pairs. Liquid water is essential for important prebiotic reactions. But equally important for the emergence of life is the contact of carbon molecules in liquid water with hot rocks and minerals. We here review the environmental conditions of the early Earth, as soon as it had liquid water at its surface and was habitable. Basing our approach to life as a “cosmic phenomenon” (de Duve 1995), i.e. a chemical continuum, we briefly address the various hypotheses for the origin of life, noting their relevance with respect to early environmental conditions. It appears that hydrothermal environments were important in this respect. We continue with the record of early life noting that, by 3.5 Ga, when the sedimentary environment started being well-preserved, anaerobic life forms had colonised all habitable microenvironments from the sea floor to exposed beach environments and, possibly, in the photic planktonic zone of the sea. Life on Earth had also evolved to the relatively sophisticated stage of anoxygenic photosynthesis. We conclude with an evaluation of the potential for habitability and colonisation of other planets and satellites in the Solar System, noting that the most common life forms in the Solar System and probably in the Universe would be similar to terrestrial chemotrophs whose carbon source is either reduced carbon or CO₂ dissolved in water and whose energy would be sourced from oxidized carbon, H₂, or other transition elements.     

Morphological Biosignatures in Early Terrestrial and Extraterrestrial Materials

Biosignatures in early terrestrial rocks are highly relevant in the search for traces of life on Mars because the early geological environments of the two planets were, in many respects, similar and, thus, the potential habitats for early life forms were similar. However, the identification and interpretation of biosignatures in ancient terrestrial rocks has proven contentious over the last few years. Recently, new investigations using very detailed field studies combined with highly sophisticated analytical techniques have begun to document a large range of biosignatures in Early Archaean rocks. Early life on Earth was diversified, widespread and relatively evolved, but its traces are generally, but not always, small and subtle. In this contribution I use a few examples of morphological biosignatures from the Early-Mid Archaean to demonstrate their variety in terms of size and type: macroscopic stromatolites from the 3.443 Ga Strelley Pool Chert, Pilbara; a meso-microscopic microbial mat from the 3.333 Ga Josefsdal Chert, Barberton; microscopic microbial colonies and a biofilm from the 3.446 Ga Kitty’s Gap Chert, Pilbara; and microscopic microbial corrosion pits in the glassy rinds of 3.22–3.48 Ga pillow lavas from Barberton. Some macroscopic and microscopic structures may be identifiable in an in situ robotic mission to Mars and in situ methods of organic molecule detection may be able to reveal organic traces of life. However, it is concluded that it will probably be necessary to return suitably chosen Martian rocks to Earth for the reliable identification of signs of life, since multiple observational and analytical methods will be necessary, especially if Martian life is significantly different from terrestrial life.     

Exobiology and Planetary Protection of icy moons

The outer solar system is an important area of investigation for exobiology, the study of life in the universe. Several moons of the outer planets involve processes and structures comparable to those thought to have played an important role in the emergence of life on Earth, such as the formation and exchange of organic materials between different reservoirs. The study of these prebiotic processes on, and in, outer solar system moons is a key goal for exobiology, together with the question of habitability and the search for evidence of past or even present life. This chapter reviews the aspects of prebiotic chemistry and potential presence of life on Europa, Enceladus and Titan, based on the most recent data obtained from space missions as well as theoretical and experimental laboratory models. The habitability of these extraterrestrial environments, which are likely to include large reservoirs of liquid water in their internal structure, is discussed as well as the particular case of Titan’s hydrocarbon lakes. The question of planetary protection, especially in the case of Europa, is also presented.     

Fossil Lipids for Life-Detection: A Case Study from the Early Earth Record

The geological preservation of lipids from the cell membranes of organisms bestows a precious record of ancient life, especially for the Precambrian eon (>542 million years ago) when Earth life was largely microbial. All organisms produce lipids that, if the lipids survive oxidative degradation, become molecular fossils entrained with information on biological diversity, environmental conditions, and post-depositional alteration history. As with most biosignatures, the molecular fossil record that is indigenous (of the same place) and syngenetic (of the same age) to host rocks can be compromised by the introduction from and reaction with foreign or younger materials (e.g., petroleum or endolithic life). Deciphering the resulting complex pool of organic signals requires tests for the provenance of molecular fossils and the overall quality of the geobiological record itself. This paper reviews the basis for the very existence of a molecular fossil record from lipid biochemistry to mechanisms of organic-matter preservation and geochemical alteration. A systematic approach to resolving the provenance of molecular fossils and historical qualities of the record is presented in a case study of an early Earth record. This example demonstrates the value of geological context and the integration of independent geobiological parameters, which are critical to the detection and understanding of the ecological processes responsible for records of life.     

Creating Habitable Zones, at all Scales, from Planets to Mud Micro-Habitats, on Earth and on Mars

The factors that create a habitable planet are considered at all scales, from planetary inventories to micro-habitats in soft sediments and intangibles such as habitat linkage. The possibility of habitability first comes about during accretion, as a product of the processes of impact and volatile inventory history. To create habitability water is essential, not only for life but to aid the continual tectonic reworking and erosion that supply key redox contrasts and biochemical substrates to sustain habitability. Mud or soft sediment may be a biochemical prerequisite, to provide accessible substrate and protection. Once life begins, the habitat is widened by the activity of life, both by its management of the greenhouse and by partitioning reductants (e.g. dead organic matter) and oxidants (including waste products). Potential Martian habitats are discussed: by comparison with Earth there are many potential environmental settings on Mars in which life may once have occurred, or may even continue to exist. The long-term evolution of habitability in the Solar System is considered.     

Electrical Effects on Atmospheric Chemistry

Electrical discharges in planetary atmospheres, and lightning in particular, can cause otherwise unexpected—but highly important—chemical species to be present. The synthesis of oxidants on Mars, nitrates on Earth and Early Mars, and of organic matter elsewhere can be driven by lightning and related electrical phenomena.     

Morphological Biosignatures from Subsurface Environments: Recognition on Planetary Missions

The Earth is inhabited by life not just at its surface, but down to a depth of kms. Like surface life, this deep subsurface life produces a fossil record, traces of which may be found in the pore space of practically all rock types. The (palaeo)subsurface of other planetary bodies is therefore a promising target in the search for another example of life. Subsurface filamentous fabrics (SFFs), i.e. mineral encrustations of a filament-based textural framework, occur in many terrestrial rocks representing present or ancient subsurface settings. SFF are interpreted as mineral encrustations on masses of filaments/pseudofilaments of microbial origin. SFF are a common example of the fossil record of subsurface life. Macroscopic (pseudostalactites, U-shapes) and microscopic (filaments) characteristics make SFF’s a biosignature that can be identified with relative ease. SFF in the subsurface are probably about as common and easily recognizable as are stromatolites in surface environments. Close-up imagers (～50 micron/pixel resolution) and microscopes (～3 micron/pixel resolution) on upcoming Mars lander missions are crucial instruments that will allow the recognition of biofabrics of surface- and subsurface origin. The resolution available however will not allow the recognition of small (～1 micron) individual mineralized microbial cells. The microscopy of unprepared rock surfaces would benefit from the use of polarizing filters to reduce surface reflectance and enhance internally reflected light. Tests demonstrate the potential to visualize mineralized filaments using this procedure.     

Schumann Resonances as a Means of Investigating the Electromagnetic Environment in the Solar System

The propagation of extremely low frequency (ELF, 3 Hz to 3 kHz) radio waves and resonant phenomena in the spherical Earth-ionosphere cavity has been studied for almost fifty years. When such a cavity is excited by naturally occurring broadband electromagnetic radiation, resonances can develop if the equatorial circumference is approximately equal to an integral number of wavelengths of the propagating electromagnetic waves; these are termed Schumann resonances. They provide information not only about thunderstorm and lightning activity on the Earth, and their relation to climate, but also on the properties of the low ionosphere. Similar investigations can be performed for any other planet or satellite, provided that it has an ionosphere. There are important differences between the Earth and other celestial bodies regarding, for example, the surface conductivity, the atmospheric conductivity profile, the geometry of the ionospheric cavity, and the sources of excitation. To a first approximation, the size of the cavity defines the fundamental resonant frequency, the atmospheric electron density profile controls the wave attenuation, the nature of the sources influences the electromagnetic field distribution in the cavity, and the body surface conductivity indicates to what extent the subsurface can be explored. The frequencies and attenuation rates of the principal eigenmodes depend upon the electrical properties of the cavity. Instruments that monitor the electromagnetic environment in the ELF range on the surface, on balloons, or on descent probes provide unique information on the cavity. In this paper, we present Schumann resonance models for selected inner planets, some gaseous giant planets and a few of their satellites. We review the crucial parameters of ELF electromagnetic waves in their atmospheric cavities, namely the electric and magnetic field spectra, their eigenfrequencies, and the associated Q-factors (damping factors). Then we present important information on theoretical developments, on a general model that uses the finite element method and on the parameterization of the cavity. Next we show the distinctiveness of each planetary environment, and discuss how ELF radio wave propagation can contribute to an assessment of the major characteristics of those planetary environments.     

Constant-False-Alarm-Rate Signal Processors for Several Types of Interference

Distribution-free methods and maximum-likelihood estimation technique have been previously suggested for constant-false-alarm-rate (CFAR) processors. The first technique assumes no a priori environmental knowledge and the second assumes almost complete environmental knowledge. Several intermediate environmental assumptions are considered. The performance of single-pulse transmission signal processors that produce CFAR for the different environments is analyzed. Probability of target detection is evaluated for Rayleigh interference and Swerling I target. It is shown that adaptive threshold techniques implemented by logarithmic amplifiers, instead of linear amplifiers, can attain better false-alarm-rate control with only small loss in target detectability.     

Gamma ray and neutron emissions from the Sun

Evidence for acceleration of charged particles in the solar atmosphere is reviewed with specific reference to production of gamma rays and neutrons at the Sun. Fluxes of these components at the Earth, based on theoretical assumptions are also reviewed and estimates and conditions for obtaining observable fluxes from Syrovatskii's dynamic dissipation model are considered. Knowledge about the Sun, to be derived from such observations, is discussed. Finally, a brief review of the present status of experimental observations and suggestions for new experimental approaches are given. Work performed while author was a guest of the Max-Planck-Institute for Physics and Astrophysics, München, on sabbatical leave from the University of New Hampshire, Durham, New Hampshire, U.S.A. Partially supported by a NATO Senior Fellowship in Science.     

Molecular Biosignatures

Life, as we know it, is based on carbon chemistry operating in an aqueous environment. Living organisms process chemicals, make copies of themselves, are autonomous and evolve in concert with the environment. All these characteristics are driven by, and operate through, carbon chemistry. The carbon chemistry of living systems is an exact branch of science and we have detailed knowledge of the basic metabolic and reproductive machinery of living organisms. We can recognise the residual biochemicals long after life has expired and otherwise lost most life-defining features. Carbon chemistry provides a tool for identifying extant and extinct life on Earth and, potentially, throughout the Universe. In recognizing that certain distinctive compounds isolable from living systems had related fossil derivatives, organic geochemists coined the term biological marker compound or biomarker (e.g. Eglinton et al. in Science 145:263–264, 1964) to describe them. In this terminology, biomarkers are metabolites or biochemicals by which we can identify particular kinds of living organisms as well as the molecular fossil derivatives by which we identify defunct counterparts. The terms biomarker and molecular biosignature are synonymous. A defining characteristic of terrestrial life is its metabolic versatility and adaptability and it is reasonable to expect that this is universal. Different physiologies operate for carbon acquisition, the garnering of energy and the storage and processing of information. As well as having a range of metabolisms, organisms build biomass suited to specific physical environments, habitats and their ecological imperatives. This overall ‘metabolic diversity’ manifests itself in an enormous variety of accompanying product molecules (i.e. natural products). The whole field of organic chemistry grew from their study and now provides tools to link metabolism (i.e. physiology) to the occurrence of biomarkers specific to, and diagnostic for, particular kinds of metabolism. Another characteristic of living things, also likely to be pervasive, is that an enormous diversity of large molecules are built from a relatively small subset of universal precursors. These include the four bases of DNA, 20 amino acids of proteins and two kinds of lipid building blocks. Third, life exploits the specificity inherent in the spatial, that is, the three-dimensional qualities of organic chemicals (stereochemistry). These characteristics then lead to some readily identifiable and measurable generic attributes that would be diagnostic as biosignatures. Measurable attributes of molecular biosignatures include:

1. Enantiomeric excess
2. Diastereoisomeric preference
3. Structural isomer preference
4. Repeating constitutional sub-units or atomic ratios
5. Systematic isotopic ordering at molecular and intramolecular levels
6. Uneven distribution patterns or clusters (e.g. C-number, concentration, δ ¹³C) of structurally related compounds.

In this paper we address details of the chemical and biosynthetic basis for these features, which largely arise as a consequence of construction from small, recurring sub-units. We also address how these attributes might become altered during diagenesis and planetary processing. Finally, we discuss the instrumental techniques and further developments needed to detect them.     

Biological implications of the Viking mission to Mars

A central purpose of Viking was to search for evidence that life exists on Mars or may have existed in the past. The missions carried three biology experiments the prime purpose of which was to seek for existing microbial life. In addition the results of a number of the other experiments have biological implications: (1) The elemental analyses of the atmosphere and the regolith showed or implied that the elements generally considered essential to terrestrial biology are present. (2) But unexpectedly, no organic compounds were detected in Martian samples by an instrument that easily detected organic materials in the most barren of terrestrial soils. (3) Liquid water is believed to be an absolute requisite for life. Viking obtained direct evidence for the presence of water vapor and water ice, and it obtained strong inferential evidence for the existence of large amounts of subsurface permafrost now and in the Martain past. However it obtained no evidence for the current existence of liquid water possessing the high chemical potential required for at least terrestrial life, a result that is consistent with the known pressure-temperature relations on the planet's surface. On the other hand, the mission did obtain strong indications from both atmospheric analyses and orbital photographs that large quantities of liquid water flowed episodically on the Martian surface 0.5 to 2.5 G years ago.The three biology experiments produced clear evidence of chemical reactivity in soil samples, but it is becoming increasingly clear that the chemical reactions were nonbiological in origin. The unexpected release of oxygen by soil moistened with water vapor in the Gas Exchange experiment together with the negative findings of the organic analysis experiment lead to the conclusion that the surface contains powerful oxidants. This conclusion is consistent with models of the atmosphere. The oxidants appear also to have been responsible for the decarboxylation of the organic nutrients that were introduced in the Label Release experiment. The major results of the GEX and LR experiments have been simulated at least qualitatively on Earth. The third, Pyrolytic Release, experiment obtained evidence for organic synthesis by soil samples. Although the mechanism of the synthesis is obscure, the thermal stability of the reaction makes a biological explanation most unlikely. Furthermore, the response of soil samples in all three experiments to the addition of water is not consistent with a biological interpretation.The conditions now known to exist at and below the Martian surface are such that no known terrestrial organism could grow and function. Although the evidence does not absolutely rule out the existence of favourable oases, it renders their existence extremely unlikely. The limiting conditions for the functioning of terrestrial organisms are not the limits for conceivable life elsewhere, and accordingly one cannot exclude the possibility that indigenous life forms may currently exist somewhere on Mars or may have existed sometime in the past. Nevertheless, the available information about the present Martian environment puts severe constraints and presents formidable challenges to any putative Martian organisms. The Martian environment in the past, on the other hand, appears to have been considerably less hostile biologically, and it might possibly have permitted the origin and transient establishment of a biota.     

Induced Magnetic Fields in Solar System Bodies

Electromagnetic induction is a powerful technique to study the electrical conductivity of the interior of the Earth and other solar system bodies. Information about the electrical conductivity structure can provide strong constraints on the associated internal composition of planetary bodies. Here we give a review of the basic principles of the electromagnetic induction technique and discuss its application to various bodies of our solar system. We also show that the plasma environment, in which the bodies are embedded, generates in addition to the induced magnetic fields competing plasma magnetic fields. These fields need to be treated appropriately to reliably interpret magnetic field measurements in the vicinity of solar system bodies. Induction measurements are particularly important in the search for liquid water outside of Earth. Magnetic field measurements by the Galileo spacecraft provide strong evidence for a subsurface ocean on Europa and Callisto. The induction technique will provide additional important constraints on the possible subsurface water, when used on future Europa and Ganymede orbiters. It can also be applied to probe Enceladus and Titan with Cassini and future spacecraft.     

On ionospheric investigations by coherent radiowaves emitted from artificial Earth satellites

Results of radio-investigations of the ionosphere with the help of coherent radiowaves emitted by beacons placed on artificial Earth satellites are given. The data discussed cover the period from 1958, after the launch of Sputniks 1 and 3, until the last years, when the geostationary satellites ATS were launched. It is shown that up to the present justice has not be done in these experiments to investigations of the local properties of the near Earth plasma. This is a great deficiency in this field of investigation. Data are given which illustrate results of investigations of local ionospheric characteristics. Such data may help to solve some problems in the present stage of the near Earth plasma study. A new possibility of radio-investigation of the near Earth plasma with the help of a chain of satellites connected together is pointed out.     

基于分布式地面站天线的空间功率合成

卫星导航系统(GNSS)地面站天线对卫星进行上行注入时,信号到达卫星时较弱,容易受到干扰,故地面站注入天线需同时具备平时多目标注入和干扰时单目标功率增强的能力。利用卫星导航系统中地面站之间能够实现精密时间同步的特点,提出了一种基于分布式卫星导航地面站抛物面天线的空间功率合成方法,使用相位预补偿实现分布式天线阵到达目标卫星信号的相位粗同步;分析了相位误差、辐射功率误差对空间功率合成效率的影响,得到了阵元初始相位标定精度与相对定位精度的约束关系;并对合成信号的抗干扰能力和信号质量进行了研究。理论和仿真结果表明,当相位精度因子小于0.2时,4个等辐射功率天线在10°仰角以上波束扫描范围内的功率合成效率均在75%以上,且可以通过控制初始相位标定精度与相对定位精度实现更高的合成效率;而在合成效率要求75%以上时,天线辐射功率误差对合成效率的影响基本可以忽略。采用分布式波束扫描天线能够对地面站上行注入进行功率增强,可实现注入波束和功率的灵活配置,有效解决制约机动式和小型化地面站功率提升的瓶颈问题。     

Cosac, The Cometary Sampling and Composition Experiment on Philae

Comets are thought to preserve the most pristine material currently present in the solar system, as they are formed by agglomeration of dust particles in the solar nebula, far from the Sun, and their interiors have remained cold. By approaching the Sun, volatile components and dust particles are released forming the cometary coma. During the phase of Heavy Bombardment, 3.8--4 billion years ago, cometary matter was delivered to the Early Earth. Precise knowledge on the physico-chemical composition of comets is crucial to understand the formation of the Solar System, the evolution of Earth and particularly the starting conditions for the origin of life on Earth. Here, we report on the COSAC instrument, part of the ESA cometary mission Rosetta, which is designed to characterize, identify, and quantify volatile cometary compounds, including larger organic molecules, by in situ measurements of surface and subsurface cometary samples. The technical concept of a multi-column enantio-selective gas chromatograph (GC) coupled to a linear reflectron time-of-flight mass-spectrometer instrument is presented together with its realisation under the scientific guidance of the Max-Planck-Institute for Solar System Research in Katlenburg-Lindau, Germany. The instrument's technical data are given; first measurements making use of standard samples are presented. The cometary science community is looking forward to receive fascinating data from COSAC cometary in situ measurements in 2014.     

Vibrational relaxation of CO2 (m,nl, p) in a CO2-N2 mixture. Part 1: Survey of available data

The knowledge of the vibrational relaxation reaction rates of diatomic and triatomic molecules is required for the modelling of numerous gaseous mixtures such as the Earth atmosphere (involving radiation transfer phenomena), exhaust plumes, afterbody wakes, Mars atmosphere, shock waves and chemical lasers. It enables a better understanding of the chemical reaction product rates and provides the populations of the radiating levels. In this study, we consider the few lowest vibrational levels of a CO₂-N₂ mixture and the V-T and V-V energy transfer processes between these levels. The reaction rates given by experimental and theoretical studies and by different surveys of data supplied by a bibliographic search are presented. Then, comparisons of the data available for the most important processes are shown. The usual assumptions concerning the vibrational relaxation of CO₂ (m, n^l, p) are recalled and briefly discussed.     

Planetary radio astronomy experiment for Voyager missions

The planetary radio astronomy experiment will measure radio spectra of planetary emissions in the range 1.2 kHz to 40.5 MHz. These emissions result from wave-particle-plasma interactions in the magnetospheres and ionospheres of the planets. At Jupiter, they are strongly modulated by the Galilean satellite Io.As the spacecraft leave the Earth's vicinity, we will observe terrestrial kilometric radiation, and for the first time, determine its polarization (RH and LH power separately). At the giant planets, the source of radio emission at low frequencies is not understood, but will be defined through comparison of the radio emission data with other particles and fields experiments aboard Voyager, as well as with optical data. Since, for Jupiter, as for the Earth, the radio data quite probably relate to particle precipitation, and to magnetic field strength and orientation in the polar ionosphere, we hope to be able to elucidate some characteristics of Jupiter auroras.Together with the plasma wave experiment, and possibly several optical experiments, our data can demonstrate the existence of lightning on the giant planets and on the satellite Titan, should it exist. Finally, the Voyager missions occur near maximum of the sunspot cycle. Solar outburst types can be identified through the radio measurements; when the spacecraft are on the opposite side of the Sun from the Earth we can identify solar flare-related events otherwise invisible on the Earth.     

Searching for Signs of Life in the Reflected Light from Exoplanets: A Catalog of Nearby Target Stars

Which stars are the best stars to search for habitable planets and signs of life? This is a trick question, because it depends not only on the kind of circumstellar environment we think is likely to be supportive to life as we know it, but it depends also on the technique being used to do the search. For example, the Catalog of Nearby Habitable Stellar Systems was designed for SETI, a search for technological signals. Because this search strategy relies on life forms out-shining their star (at least at certain frequencies), target selection is not complicated by the need to spatially resolve the habitable planets on which these life forms presumably live. On the other hand, because the life forms being sought are technologically advanced, it seems reasonable to assume that their planet had to be continuously habitable for long enough to evolve such biological complexity. Thus the deciding factor for SETI is that of long term habitability. Meanwhile, other missions to directly detect habitable planets (e.g., NASA’s TPF and ESA’s Darwin) are less worried about long term habitability but must struggle with the competing factors of planet separation from the star and planet brightness relative to the star. This paper outlines a variety of challenges in the search for simple and complex life in the Solar Neighborhood.