Gravity effects on biological systems.

Theoretical investigations of the membrane-solution interface predict different effects of gravity on vertically and horizontally oriented planar membranes. Single channel events of gramicidin incorporated into phosphatidylserine planar bilayer membranes were measured in 0.1 M KCl solution, pH 7, at room temperature. The potential difference across the membrane was set to +/- 70 mV. The mean channel current was observed to be about 20% higher in horizontally oriented membranes compared to vertical membranes. This is in good agreement with the theoretical considerations and demonstrates that gravity does affect membrane processes by interaction with the membrane-solution interface which is a ubiquitous structure in biological systems.     

Gravity and the membrane-solution interface: theoretical investigations.

The theory of concentration and potential variations at interfaces is applied to the membrane-solution interface to calculate density variations. The theory is modified to take care of the finite ion volumes in electrolytes. Our model is a phospholipid membrane with a surface charge density of -4.824*10(-6)(As/cm2) in contact with solutions of KCl, NaCl, CaCl2, and mixtures. Maximal density variations of about 4*10(-2)(G/cm3) were found in surface layers between the membrane and the solutions. The extension of the layers is in the range of 1 to 6 nm.     

Polycyclic aromatic hydrocarbons: primitive pigment systems in the prebiotic environment.

Polycyclic aromatic hydrocarbons (PAH) in the form of polymerized derivatives represent over 90% of the organic material of carbonaceous chondrites. It now appears likely that there was substantial survival of the organic content of meteoritic and cometary infall during late accretion, so that PAH would presumably be major components of the organic inventory present on the prebiotic Earth. An important question relative to chemical evolution and energy transduction is the nature of pigments which could be available to make light energy available to the earliest cellular forms of life. PAH and their derivatives all absorb light in the near UV and blue wavelengths, and are candidates for primitive pigments. We have explored this possibility in a model system consisting of mixtures of pyrene, fluoranthene and pyrene derivatives with hexadecane, dispersed in dilute salt solutions. Upon illumination, photochemical oxidation of the hexadecane occurs, with long-chain amphiphiles such as 2-hexadecanone and 2-hexadecanol as products. Because the reaction proceeds under strictly anaerobic conditions, the source of oxygen is apparently water. We also observed acid pH shifts during illumination. Photochemical production of hydrogen ion is significant, in that chemiosmotic proton gradients across membranes are used by all contemporary cells as a source of energy for ATP synthesis and nutrient transport. To test whether the protons could be used to transduce light energy into a useful form, PAH derivatives were included in lipid bilayer membranes (liposomes). Upon illumination, protons (or acidic products) were produced and accumulated inside the vesicles, so that substantial pH gradients were established across the membranes, acid inside. We conclude that PAH dissolved in aliphatic hydrocarbons absorb light energy and use it to oxidize the hydrocarbon to long-chain amphiphilic molecules. The oxidation is accompanied by release of protons. If PAH derivatives are included in the bilayer membrane of lipid vesicles, protons accumulate within the membrane-bounded volumes to form proton gradients. This system provides a useful model of a primitive photochemical reaction in which light energy is transduced into potentially useable forms.     

Effect of the troposphere on surface neutron counter measurements

Surface neutron counter data are often used as a proxy for atmospheric ionisation from cosmic rays in studies of extraterrestrial effects on climate. Neutron counter instrumentation was developed in the 1950s and relationships between neutron counts, ionisation and meteorological conditions were investigated thoroughly using the techniques available at the time; the analysis can now be extended using modern data. Whilst surface neutron counts are shown to be a good proxy for ionisation rate, the usual meteorological correction applied to surface neutron measurements, using surface atmospheric pressure, does not completely compensate for tropospheric effects on neutron data. Residual correlations remain between neutron counts, atmospheric pressure and geopotential height, obtained from meteorological reanalysis data. These correlations may be caused by variations in the height and temperature of the atmospheric layer at ∼100 hPa. This is where the primary cosmic rays interact with atmospheric air, producing a cascade of secondary ionising particles.     

Effect of gravity on the velocity of a crystallization front

The crystal growth from a liquid can be studied by methods of non-equilibrium thermodynamics of a discontinuity surface. These methods are used to derive the dependence of the crystalization velocity on transport and surface phenomena across and along an interface solid-liquid and on the acceleration of gravity.     

The plasma sheet and boundary layers under northward IMF: A multi-point and multi-instrument perspective

During conditions of northward interplanetary magnetic field (IMF), the near-tail plasma sheet is known to become denser and cooler, and is described as the cold-dense plasma sheet (CDPS). While its source is likely the solar wind, the prominent penetration mechanisms are less clear. The two main candidates are solar wind direct capture via double high-latitude reconnection on the dayside and Kelvin–Helmholtz/diffusive processes at the flank magnetopause. This paper presents a case study on the formation of the CDPS utilizing a wide variety of space- and ground-based observations, but primarily from the Double Star and Polar spacecraft on December 5th, 2004. The pertinent observations can be summarized as follows: TC-1 observes quasi-periodic (∼2 min period) cold-dense boundary layer (compared to a hot-tenuous plasma sheet) signatures interspersed with magnetosheath plasma at the dusk flank magnetopause near the dawn-dusk terminator. Analysis of this region suggests the boundary to be Kelvin–Helmholtz unstable and that plasma transport is ongoing across the boundary. At the same time, IMAGE spacecraft and ground based SuperDARN measurements provide evidence of high-latitude reconnection in both hemispheres. The Polar spacecraft, located in the southern hemisphere afternoon sector, sunward of TC-1, observes a persistent boundary layer with no obvious signature of boundary waves. The plasma is of a similar appearance to that observed by TC-1 inside the boundary layer further down the dusk flank, and by TC-2 in the near-Earth magnetotail. We present comparisons of electron phase space distributions between the spacecraft. Although the dayside boundary layer at Polar is most likely formed via double high-altitude reconnection, and is somewhat comparable to the flank boundary layer at Double Star, some differences argue in favour of additional transport that augment solar wind plasma entry into the tail regions.     

Studies of the interaction of gravity with biological membranes using alamethicin doped planar lipid bilayers as a model system.

Gravity interacts with biological systems on different levels of complexity. For the understanding of the action of gravity on such systems at higher degrees of organisation, the investigation of interactions on the membrane and even on the molecular level is crucial. To do such studies, planar lipid bilayers with incorporated transport mediating molecules, i.e. membranes of defined biochemical composition, are close to perfect model systems. In our experiments we have used painted planar lipid bilayers doped with alamethicin. Alamethicin is especially suitable for such studies because of its high sensitivity to applied external forces, which is a result of its special pore forming mechanism. Additional, different to most other transport mediating molecules, a big amount of data from the literature is available about the dependency of alamethicin pores on physical and chemical membrane parameters. We found that the conductance of alamethicin doped bilayers is dependent on the angle of the bilayer with the gravitational vector and that it furthermore can be reduced significantly under hyper gravity conditions in a centrifuge. The effect of gravity here is not an effect on the pore conductance or the membrane-aqueous solution interface, but it is due to an interaction of gravity with the pore forming mechanism, as can be shown by investigating the dependency of the alamethicin pore kinetics on the applied forces.     

Dependence of E-sporadic layer response on solar and geomagnetic activity variations from its ion composition

The aim of this paper is to consider an influence of solar and geomagnetic activity level variations on frequency and stochastic parameters of mid-latitude ionosphere sporadic-E layer. Critical frequency of sporadic-E layer, foEs, relative excess of sporadic ionization over monthly median values, foEs − foEm/foEm, and probability of Es layer appearance, PEs, are considered. It has been found that sporadic-E layer parameters response to solar and geomagnetic activity level variations can be both positive (foEs and PEs values are increase) and negative (foEs and PEs values are decrease). In particular, sporadic-E layers response to solar and geomagnetic activity variations are different depending upon layer intensity. It is suggested that revealed differences may be associated with dissimilarity of the layers ion composition (high-intensive layers are composed from metallic ions and low intensive composed from molecular ions).     

Electron densities and temperatures in the Venus ionosphere: Effects of solar EUV,solar wind pressure and magnetic field

The Venus ionosphere is influenced by variations in both solar EUV flux and solar wind conditions. On the dayside the location of the topside of the ionosphere, the ionopause, is controlled by solar wind dynamic pressure. Within the dayside ionosphere, however, electron density is affected mainly by solar EUV variations, and is relatively unaffected by solar wind variations and associated magnetic fields induced within the ionosphere. The existence of a substantial nightside ionosphere of Venus is thought to be due to the rapid nightward transport of dayside ionospheric plasma across the terminator. Typical solar wind conditions do not strongly affect this transport and consequently have little direct influence on nightside ionospheric conditions, except on occasions of extremely high solar wind dynamic pressure. However, both nightside electron density and temperature are affected by the presence of magnetic field, as in the case of ionospheric holes.     

Meteorological Research Institute multivariate ocean variational estimation (MOVE) system: Some early results

The Meteorological Research Institute multivariate ocean variational estimation (MOVE) System has been developed as the next-generation ocean data assimilation system in Japan Meteorological Agency. A multivariate three-dimensional variational (3DVAR) analysis scheme with vertical coupled temperature–salinity empirical orthogonal function modes is adopted. The MOVE system has two varieties, the global (MOVE-G) and North Pacific (MOVE-NP) systems. The equatorial Pacific and western North Pacific are analyzed with assimilation experiments using MOVE-G and -NP, respectively. In each system, the salinity and velocity fields are well reproduced, even in cases without salinity data. Changes in surface and subsurface zonal currents during the 1997/98 El Niño event are captured well, and their transports are reasonably consistent with in situ observations. For example, the eastward transport in the upper layer around the equator has 70 Sv in spring 1997 and weakens in spring 1998. With MOVE-NP, the Kuroshio transport has 25 Sv in the East China Sea, and 40 Sv crossing the ASUKA (Affiliated Surveys of the Kuroshio off Cape Ashizuri) line south of Japan. The variations in the Kuroshio transports crossing the ASUKA line agree well with observations. The Ryukyu Current System has a transport ranging from 6 Sv east of Taiwan to 17 Sv east of Amami. The Oyashio transport crossing the OICE (Oyashio Intensive observation line off Cape Erimo) line south of Hokkaido has 14 Sv southwestward (near shore) and 11 Sv northeastward (offshore). In the Kuroshio–Oyashio transition area east of Japan, the eastward transport has 41 Sv (32–36°N) and 12 Sv (36–39°N) crossing the 145°E line.     

高超声速尖锥边界层转捩数值模拟

边界层转捩对高超声速飞行器气动力和热产生重要影响.通过联立求解Favré平均层流脉动能方程与Favré平均Navier-Stokes方程,开展了高马赫数条件下尖锥边界层转捩位置的数值预测研究.将数值计算结果与可用的实验结果进行对比,结果表明增大单位Reynolds数可使转捩提前发生,攻角效应可使迎风面转捩延迟,背风面转捩提前.     

Thermal and thermosolutal convective effects in bridgman solidification: Effect of orientation relative to gravity

A concentrated binary system (Ge-Si) and a dilute one (Ge-Ga) have been solidified at 1 g, in a new vertical Bridgman furnace where radial thermal gradients have been minimized. Very different solutal boundary layer extents (δ_Ge-Si = 3 cm, δ_Ge-Ga = 2.5 mm) obtained for both systems solidified in the same thermal conditions are explained by simple analytical hydrodynamic models. It is demonstrated that :1. The convective transport of Ga in the Ge-Ga system is due to the unavoidable residual horizontal thermal gradients associated with discontinuity of thermal properties at the solid-liquid interface.2. The larger boundary layer extent observed for Ge-Si and the corresponding pure diffusive transport is the result of the stabilizing effect of the longitudinal solutal gradient in this system.Thus, in the case of dilute systems, convective effects can only be cancelled through a reduction of the gravity level. On the opposite, pure diffusive solute transport can be achieved in the vertical configuration a 1 g in the case of concentrated systems where a stabilizing solutal effect is operating.     

Free and membrane-bound calcium in microgravity and microgravity effects at the membrane level.

The changes of [Ca2+]i controlled is known to play a key regulatory role in numerous cellular processes especially associated with membranes. Previous studies from our laboratory have demonstrated an increase in calcium level in root cells of pea seedlings grown aboard orbital station "Salyut 6". These results: 1) indicate that observed Ca(2+)-binding sites of membranes also consist in proteins and phospholipids; 2) suggest that such effects of space flight in membrane Ca-binding might be due to the enhancement of Ca2+ influx through membranes. In model presented, I propose that Ca(2+)-activated channels in plasma membrane in response to microgravity allow the movement of Ca2+ into the root cells, causing a rise in cytoplasmic free Ca2+ levels. The latter, in its turn, may induce the inhibition of a Ca2+ efflux by Ca(2+)-activated ATPases and through a Ca2+/H+ antiport. It is possible that increased cytosolic levels of Ca2+ ions have stimulated hydrolysis and turnover of phosphatidylinositols, with a consequent elevation of cytosolic [Ca2+]i. Plant cell can response to such a Ca2+ rise by an enhancement of membranous Ca(2+)-binding activities to rescue thus a cell from an abundance of a cytotoxin. A Ca(2+)-induced phase separation of membranous lipids assists to appear the structure nonstable zones with high energy level at the boundary of microdomains which are rich by some phospholipid components; there is mixing of molecules of the membranes contacted in these zones, the first stage of membranous fusion, which was found in plants exposed to microgravity. These results support the hypothesis that a target for microgravity effect is the flux mechanism of Ca2+ to plant cell.     

Sensing snow height and surface temperature variations in Greenland from GPS reflected signals

The in situ measurements of snow surface temperature (SST) and snow height (SH) are very difficult with high costs, particularly in Greenland Ice Sheet (GrIS). Since the snow depth variations coupling with surface temperature are related to GPS multipath, it is possible to estimate the snow depth and surface air temperature variations by incorporating GPS-Reflectometry (GPS-R). In this paper, the reflected signals from ground GPS receivers are used to sense the SST and SH variations based on the thermophysical behavior and variations of snow layer from April to June 2010 at SMM1 site and from March to December 2010 at MARG site in Greenland. The results show that the mean daily changes in the ionospheric geometrical-free linear combination (GPS-L4) of dual-frequency GPS signals are related to daily SST and SH variations. The nonparametric bootstrapping model in direct (forward) and inverse models are developed and applied to estimate the SST and SH variations. The mean biases of SST and SH estimates are 0.18 °C and 0.23 m at SMM1 site, respectively, and 3.8 °C and 0.13 m at MARG site, respectively.     

A model for calculating the vertical distribution of the atmospheric electric potential in the exchange layer in a maritime clean atmosphere

A theoretical model is developed for calculating the vertical distribution of atmospheric electric potential in exchange layer of maritime clean atmosphere. The transport of space charge in electrode layer acts as a convective generator in this model and plays a major role in determining potential distribution in vertical. Eddy diffusion is the main mechanism responsible for the distribution of space charge in vertical. Our results show that potential at a particular level increases with increase in the strength of eddy diffusion under similar conditions. A method is suggested to estimate columnar resistance, the ionospheric potential and the vertical atmospheric electric potential distribution in exchange layer from measurements of total air-earth current density and surface electric field made over oceans. The results are validated and found to be in very good agreement with the previous aircraft measurements. Different parameters involved in the proposed methodology can be determined either theoretically, as in the present work, or experimentally using the near surface atmospheric electrical measurements or using some other surface-based measurement technique such as LIDAR. A graphical relationship between the atmospheric eddy diffusion coefficient and height of exchange layer obtained from atmospheric electrical approach, is reported.     

Liposome formation in microgravity.

Liposomes are artificial vesicles with a phospholipid bilayer membrane. The formation of liposomes is a self-assembly process that is driven by the amphipathic nature of phospholipid molecules and can be observed during the removal of detergent from phospholipids dissolved in detergent micelles. As detergent concentration in the mixed micelles decreases, the non-polar tail regions of phospholipids produce a hydrophobic effect that drives the micelles to fuse and form planar bilayers in which phospholipids orient with tail regions to the center of the bilayer and polar head regions to the external surface. Remaining detergent molecules shield exposed edges of the bilayer sheet from the aqueous environment. Further removal of detergent leads to intramembrane folding and membrane folding and membrane vesiculation, forming liposomes. We have observed that the formation of liposomes is altered in microgravity. Liposomes that were formed at 1-g did not exceed 150 nm in diameter, whereas liposomes that were formed during spaceflight exhibited diameters up to 2000 nm. Using detergent-stabilized planar bilayers, we determined that the stage of liposome formation most influenced by gravity is membrane vesiculation. In addition, we found that small, equipment-induced fluid disturbances increased vesiculation and negated the size-enhancing effects of microgravity. However, these small disturbances had no effect on liposome size at 1-g, likely due to the presence of gravity-induced buoyancy-driven fluid flows (e.g., convection currents). Our results indicate that fluid disturbances, induced by gravity, influence the vesiculation of membranes and limit the diameter of forming liposomes.     

Interplanetary crew doses and dose equivalents: variations among different bone marrow and skin sites.

Previously, calculations of bone marrow dose from the large solar particle event (SPE) of July 2000 were carried out using the BRYNTRN space radiation transport code and the computerized anatomical man (CAM) model. Results indicated that the dose for a bone marrow site in the mid-thigh might be twice as large as the dose for a site in the pelvis. These large variations may be significant for space radiation protection purposes, which traditionally use an average of many (typically 33) sites throughout the body. Other organs that cover large portions of the body, such as the skin, may also exhibit similar variations with doses differing from site to site. The skin traditionally uses an average of 32 sites throughout the body. Variations also occur from site to site among the dose equivalents, which may be important in determining stochastic effects. In this work, the magnitudes of dose and dose equivalent variations from site to site are investigated. The BRYNTRN and HZETRN transport codes and the CAM model are used to estimate bone marrow and skin doses and dose equivalents as a function of position in the body for several large solar particle events and annual galactic cosmic ray spectra from throughout the space era. These position-specific results are compared with the average values usually used for radiation protection purposes. Various thicknesses of aluminum shielding, representative of nominal spacecraft, are used in the analyses.     

Molecular aspects of adaptation to extreme cold environments

Here are reviewed and summarized the strategies adopted by living organisms to survive low temperatures, from a molecular and membrane point of view. The presentation is aimed at a wide variety of readers.

Two prime examples of connections between biological cold adaptation and the molecular level are (1) antifreeze proteins in fish from cold sea water, (the DNA sequence of the protein gene is now known) (2) the fluidity characteristics of cell membranes in a wide variety of organisms. In model membranes of phospholipids, stabler “s-phases” have recently been found to form at low temperatures. Antarctic endolithic organisms, living just under the surface of rocks, are exposed to long periods of low temperatures, and may develop such phases in their membranes. In the saturated phosphatidyl cholines, only lipids with a restricted range of acyl chain lengths show simultaneously s-phases and a main transition : This restricted range is about the restricted range found in natural membranes. The s-phases also form in the presence of natural cryoprotectants, and may be connected with botanical vernalization.     

带QoP反馈的实时视频服务质量控制框架

分析了实时视频在现有"尽力而为"IP网络上传输所面临的带宽、延迟和丢失等问题,介绍了需要采用的拥塞控制和差错控制等应用级服务质量控制策略,并提出了一种联合的支持应用级服务质量控制的实时视频传输框架.该框架既考虑了网络资源的动态变化和影响,又兼顾了视频的服务质量调整对视频的表现质量的限制因素,充分利用了服务质量和表现质量之间的映射关系,具有更广泛的实用价值.      

阵列射流冲击复合不同肋化表面的沸腾特性

阵列射流冲击冷却技术可以有效地解决高热流密度器件的散热问题,为了验证受冲击表面强化传热结构对优化两相射流冷却性能的有效性,结合高速显微摄像手段,研究了不同肋化表面结构形态对受限式阵列射流冷却的流动、传热特性的影响。设计了2种含不同肋化表面形态:光滑切割针肋（0.6 mm×0.6 mm×1.0 mm）、外覆多孔烧结层的粗糙针肋（粒径为73~53 μm）。实验使用无水乙醇为工质,以光滑表面的射流冷却热沉为对照组,入口温度均为20℃,在固定工质流量7.5 mL/s下,随着加热热流密度由5 W/cm2增加至100 W/cm2时,热沉的换热系数均持续上升但增幅逐渐减小,未明显观察到沸腾相变的发生。对固定热流密度82.6 W/cm2、80.5 W/cm2改变工质流量（射流雷诺数）的实验工况,当工质流量由7.5 mL/s逐渐降低至1.0 mL/s时,可以非常明显地观测到射流腔内部工质由分层湍流逐步进入泡状流、弹状流及环状流,其分别对应起始沸腾区、核态沸腾区及膜态沸腾区。