Surface characteristics of spacecraft components affect the aggregation of microorganisms and may lead to different survival rates of bacteria on Mars landers

Layers of dormant endospores of Bacillus subtilis HA101 were applied to eight different spacecraft materials and exposed to martian conditions of low pressure (8.5 mbar), low temperature (-10 degrees C), and high CO(2) gas composition and irradiated with a Mars-normal ultraviolet (UV-visible- near-infrared spectrum. Bacterial layers were exposed to either 1 min or 1 h of Mars-normal UV irradiation, which simulated clear-sky conditions on equatorial Mars (0.1 tau). When exposed to 1 min of Mars UV irradiation, the numbers of viable endospores of B. subtilis were reduced three to four orders of magnitude for two brands of aluminum (Al), stainless steel, chemfilm-treated Al, clear-anodized Al, and black-anodized Al coupons. In contrast, bacterial survival was reduced only one to two orders of magnitude for endospores on the non-metal materials astroquartz and graphite composite when bacterial endospores were exposed to 1 min of Mars UV irradiation. When bacterial monolayers were exposed to 1 h of Mars UV irradiation, no viable bacteria were recovered from the six metal coupons listed above. In contrast, bacterial survival was reduced only two to three orders of magnitude for spore layers on astroquartz and graphite composite exposed to 1 h of Mars UV irradiation. Scanning electron microscopy images of the bacterial monolayers on all eight spacecraft materials revealed that endospores of B. subtilis formed large aggregates of multilayered spores on astroquartz and graphite composite, but not on the other six spacecraft materials. It is likely that the formation of multilayered aggregates of endospores on astroquartz and graphite composite is responsible for the enhanced survival of bacterial cells on these materials.     

Survival of Bacillus subtilis endospores on ultraviolet-irradiated rover wheels and Mars regolith under simulated Martian conditions

Endospores of Bacillus subtilis HA101 were applied to a simulated Mars Exploration Rover (MER) wheel and exposed to Mars-normal UV irradiation for 1, 3, or 6 h. The experiment was designed to simulate a contaminated rover wheel sitting on its landing platform before rolling off onto the martian terrain, as was encountered during the Spirit and Opportunity missions. When exposed to 1 h of Mars UV, a reduction of 81% of viable endospores was observed compared to the non-UV irradiated controls. When exposed for 3 or 6 h, reductions of 94.6% and 96.6%, respectively, were observed compared to controls. In a second experiment, the contaminated rover wheel was rolled over a bed of heat-sterilized Mars analog soil; then the analog soil was exposed to full martian conditions of UV irradiation, low pressure (6.9 mbar), low temperature (-10°C), and an anaerobic CO(2) martian atmosphere for 24 h to determine whether endospores of B. subtilis on the contaminated rover wheel could be transferred to the surface of the analog soil and survive martian conditions. The experiment simulated conditions in which a rover wheel might come into contact with martian regolith immediately after landing, such as is designed for the upcoming Mars Science Laboratory (MSL) rover. The contaminated rover wheel transferred viable endospores of B. subtilis to the Mars analog soil, as demonstrated by 31.7% of samples showing positive growth. However, when contaminated soil samples were exposed to full martian conditions for 24 h, only 16.7% of samples exhibited positive growth-a 50% reduction in the number of soil samples positive for the transferred viable endospores.     

火星表面热辐射环境模拟

为了对火星表面的热辐射环境进行模拟,以辅助火星探测等任务,建立了火星大气的一维模型和土壤的一维导热模型,并与NASA的一维火星大气辐射程序相结合,得到了一套整体模拟系统。模拟获得了火星地表温度及接收到的可见光、红外辐射热流密度,分析了季节、纬度、尘暴、云层的变化对地表温度和所受太阳辐射造成的影响。模拟结果表明,纬度和季节的变化影响着太阳高度角和日照时长等因素,进而对可见光辐射造成显著影响;尘埃光学厚度的增加会削弱可见光辐射并增强红外辐射,云层光学性质的改变造成的影响与之相似但较小;四者的改变都会对地表的温度及接收到的太阳辐射热流密度造成不同程度的影响。     

低气压温控设备的舱内环境温度影响因素分析

低气压温控设备用于模拟临近空间低气压和高低温环境,低气压环境的温度场控制是低气压温控设备的关键指标,也是设计难点。文章首先通过Fluent软件对无风扇扰动的低气压舱中的环境温度进行数值仿真,研究热沉开孔对舱内环境温度的影响;在低气压舱模型中增加风扇模型,分析强制换热对低气压舱内环境温度的影响。通过在低气压温控设备中进行的无强制对流的温度控制试验,研究此时空间温度的降温速率、试验舱压力和舱内试件工装对舱内环境温度的影响。数值计算和试验研究结果表明,热沉开孔、扰动风扇、试验舱压力和试件工装均对低气压舱内环境温度有影响,在低气压温控设备的研制中,需综合考虑上述因素。     

火星大气来流模拟装置CFD仿真与试验

根据我国火星着陆巡视器工作过程,其着陆发动机需要在相对火星大气高速迎风运动中可靠点火。由于巡视器着陆时发动机喷管出口气流与火星稀薄气流方向相反,目前无法通过理论计算准确获得着陆过程的动态流场对发动机起动过程的影响量值。为验证火星着陆环境下发动机点火的适应性,需要建立发动机的火星大气来流试验环境模拟条件。为模拟发动机在火星大气条件下的相对运动,在真空舱内发动机保持固定,前端设置环形来流形成装置,该装置在发动机喷管周围形成一定速度的逆向来流包络。采用数值模拟技术结合试验验证方法,在火星着陆器巡视器主发动机性能考核试验中,针对来流的形成装置开展了设计研究工作。来流模拟试验测试数据表明:在确保贮箱供应压力稳定的条件下,来流模拟系统能够形成100~200 m/s速度的稳定来流,发动机在来流下能稳定启动工作,真空舱压力满足试验要求。     

一种三元结构的火星采样返回任务方案分析

介绍了目前国外提出的一种三元结构的火星采样返回任务方案,整个方案分3次发射,分别发射漫游车、着陆器和轨道器,每次发射间隔为4年,最终目的是将火星样品带回地球。该方案的优势在于,通过3次发射分别完成漫游车巡视勘察、着陆器现场探测、轨道器数据中继和在轨探测,最终综合完成火星采样返回,能够极大地缓解项目进度和资金压力,充分利用每次发射窗口分步骤完成探测任务。文章重点对方案涉及的关键技术进行了分析,包括样品获取与封装、行星保护、精准着陆、漫游车的危险规避能力和移动性、火星上升器、交会与样品捕获、地球再入器技术等;对方案的前景和优势进行了探讨,并给出几点启示,如精准着陆或成为今后行星探测着陆方式的新趋势,火星采样返回任务将是人类火星探测的里程碑,今后的深空探测任务趋向国际合作模式等。     

Conceptual study and key technology development for Mars Aeroflyby sample collection

Conceptual study of Mars Aeroflyby Sample Collection (MASC) is conducted as a part of the next Mars exploration mission currently entertained in Japan Aerospace Exploration Agency. In the mission scenario, an atmospheric entry vehicle is flown into the Martian atmosphere, collects the Martian dust particles as well as atmospheric gases during the guided hypersonic flight, exits the Martian atmosphere, and is inserted into a parking orbit from which a return system departs for the earth to deliver the dust and gas samples. In order to accomplish a controlled flight and a successful orbit insertion, aeroassist orbit transfer technologies are introduced into the guidance and control system. System analysis is conducted to assess the feasibility and to make a conceptual design, finding that the MASC system is feasible at the minimum system mass of 600 kg approximately. The aerogel, which is one of the candidates for the dust sample collector, is assessed by arcjet heating tests to examine its behavior when exposed to high-temperature gases, as well as by particle impingement tests to evaluate its dust capturing capability.     

Mars orbiter insertion by use of atmospheric deceleration

The multistep adaptive control algorithms with trajectory prediction for Mars orbiter insertion by use of atmospheric deceleration are described. The navigation task is proposed to be solved simultaneously with defining the control law throughout the entry. The stabilization algorithm ensures a low fuel consumption and acceptability of transient process, even under a considerable disturbing aerodynamic torque. Digital computer simulation has been used for the algorithm development and evaluation. The simulation results have shown high effectiveness of the proposed control methods.     

Regional and seasonal limitations for Mars intrinsic ecopoiesis

Mars ecopoiesis is a human controlled process consisting in changes needed for anaerobic life to be established on planet surface. The daily minimum temperature on present day Mars is well below the water freezing point, due to the low thermal inertia of the surface. A simple time-dependent model to evaluate the ground temperature is developed here. It takes into account the incident solar radiation, the greenhouse effect and surface thermal inertia. The model is applied to two modified Martian atmospheres. Increasing surface thermal inertia seems to be necessary for Mars intrinsic ecopoiesis. This can be done either by removing the regolith layer covering the bedrock or by regolith compression. The Northern hemisphere of the terraformed Mars appears to be more hospitable than the Southern hemisphere, because the amplitude of the daily temperature excursion there is lower and the freezing temperature appears at higher latitudes. A regional (and seasonal) terraforming of Mars is suggested.     

Exposure of Arabidopsis thaliana to hypobaric environments: implications for low-pressure bioregenerative life support systems for human exploration missions and terraforming on Mars

Understanding how hypobaria can affect net photosynthetic (P (net)) and net evapotranspiration rates of plants is important for the Mars Exploration Program because low-pressured environments may be used to reduce the equivalent system mass of near-term plant biology experiments on landers or future bioregenerative advanced life support systems. Furthermore, introductions of plants to the surface of a partially terraformed Mars will be constrained by the limits of sustainable growth and reproduction of plants to hypobaric conditions. To explore the effects of hypobaria on plant physiology, a low-pressure growth chamber (LPGC) was constructed that maintained hypobaric environments capable of supporting short-term plant physiological studies. Experiments were conducted on Arabidopsis thaliana maintained in the LPGC with total atmospheric pressures set at 101 (Earth sea-level control), 75, 50, 25 or 10 kPa. Plants were grown in a separate incubator at 101 kPa for 6 weeks, transferred to the LPGC, and acclimated to low-pressure atmospheres for either 1 or 16 h. After 1 or 16 h of acclimation, CO(2) levels were allowed to drawdown from 0.1 kPa to CO(2) compensation points to assess P (net) rates under different hypobaric conditions. Results showed that P (net) increased as the pressures decreased from 101 to 10 kPa when CO(2) partial pressure (pp) values were below 0.04 kPa (i.e., when ppCO2 was considered limiting). In contrast, when ppCO(2) was in the nonlimiting range from 0.10 to 0.07 kPa, the P (net) rates were insensitive to decreasing pressures. Thus, if CO(2 )concentrations can be kept elevated in hypobaric plant growth modules or on the surface of a partially terraformed Mars, P (net) rates may be relatively unaffected by hypobaria. Results support the conclusions that (i) hypobaric plant growth modules might be operated around 10 kPa without undue inhibition of photosynthesis and (ii) terraforming efforts on Mars might require a surface pressure of at least 10 kPa (100 mb) for normal growth of deployed plant species.     

Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia

Bacterial endospores in the genus Bacillus are considered good models for studying interplanetary transfer of microbes by natural or human processes. Although spore survival during transfer itself has been the subject of considerable study, the fate of spores in extraterrestrial environments has received less attention. In this report we subjected spores of a strain of Bacillus subtilis, containing luciferase resulting from expression of an sspB-luxAB gene fusion, to simulated martian atmospheric pressure (7-18 mbar) and composition (100% CO(2)) for up to 19 days in a Mars simulation chamber. We report here that survival was similar between spores exposed to Earth conditions and spores exposed up to 19 days to simulated martian conditions. However, germination-induced bioluminescence was lower in spores exposed to simulated martian atmosphere, which suggests sublethal impairment of some endogenous spore germination processes.     

The Mars Sample Return Project.

The Mars Sample Return (MSR) Project is underway. A 2003 mission to be launched on a Delta III Class vehicle and a 2005 mission launched on an Ariane 5 will culminate in carefully selected Mars samples arriving on Earth in 2008. NASA is the lead agency and will provide the Mars landed elements, namely, landers, rovers, and Mars ascent vehicles (MAVs). The French Space Agency CNES is the largest international partner and will provide for the joint NASA/CNES 2005 Mission the Ariane 5 launch and the Earth Return Mars Orbiter that will capture the sample canisters from the Mars parking orbits the MAVs place them in. The sample canisters will be returned to Earth aboard the CNES Orbiter in the Earth Entry Vehicles provided by NASA. Other national space agencies are also expected to participate in substantial roles. Italy is planning to provide a drill that will operate from the Landers to provide subsurface samples. Other experiments in addition to the MSR payload will also be carried on the Landers. This paper will present the current status of the design of the MSR missions and flight articles.     

Mitigation of the impact of terrestrial contamination on organic measurements from the Mars Science Laboratory

The objective of the 2009 Mars Science Laboratory (MSL), which is planned to follow the Mars Exploration Rovers and the Phoenix lander to the surface of Mars, is to explore and assess quantitatively a site on Mars as a potential habitat for present or past life. Specific goals include an assessment of the past or present biological potential of the target environment and a characterization of its geology and geochemistry. Included in the 10 investigations of the MSL rover is the Sample Analysis at Mars (SAM) instrument suite, which is designed to obtain trace organic measurements, measure water and other volatiles, and measure several light isotopes with experiment sequences designed for both atmospheric and solid-phase samples. SAM integrates a gas chromatograph, a mass spectrometer, and a tunable laser spectrometer supported by sample manipulation tools both within and external to the suite. The sub-part-per-billion sensitivity of the suite for trace species, particularly organic molecules, along with a mobile platform that will contain many kilograms of organic materials, presents a considerable challenge due to the potential for terrestrial contamination to mask the signal of martian organics. We describe the effort presently underway to understand and mitigate, wherever possible within the resource constraints of the mission, terrestrial contamination in MSL and SAM measurements.     

Terraforming Mars: conceptual solutions to the problem of plant growth in low concentrations of oxygen

The widespread growth of higher plants on Mars following ecopoiesis has often been invoked as a method of generating atmospheric oxygen. However, one issue that has been overlooked in this regard is the fact that terrestrial plants do not thrive under conditions of low oxygen tension. A review of the relevant botanical literature reveals that the high oxygen demands of root respiration could limit the introduction of most plants on Mars until after terraforming has raised the atmospheric pO2 to 20-100 mbar. A variety of physiological strategies are discussed which, if it is possible to implement them in a genetically engineered plant specifically designed for life on Mars, might allow this problem to be overcome.     

The photochemical stability of carbonates on Mars

Carbonates, predominately MgCO3, have been spectroscopically identified at a level of 2-5% in martian dust. However, in spite of this observation, and a large number of climate studies that suggest 1 to several bars of CO2 should be sequestered in carbonate rocks, no outcrop-scale exposures of carbonate have been detected anywhere on Mars to date. To address one hypothesis for this long-standing puzzle, the effect of ultraviolet (UV) light on the stability of calcium carbonate in a simulated martian atmosphere was experimentally investigated. Using 13C-labeled calcite, we found no experimental evidence of the UV photodecomposition of calcium carbonate in a simulated martian atmosphere. Extrapolating the lower limit of detection of our experimental system to an upper limit of carbonate decomposition on Mars yields a quantum efficiency of 3.5 x 10(-8) molecules/photon over the wavelength interval of 190-390 nm and a maximum UV photodecomposition rate of 1.2 x 10(-13) kg m(-2) s(-1) from a calcite surface. The maximum loss of bulk calcite due to this process would be 2.5 nm year(-1) (Mars year). However, calcite is expected to be thermodynamically stable on the surface of Mars, and potential UV photodecomposition reaction mechanisms indicate that, though calcium carbonate may decompose under vacuum, it would be stable in a CO2 atmosphere. Given the expected stability of carbonate on Mars and our inability to detect carbonate decomposition, we conclude that it is unlikely that the apparent absence of extensive carbonate deposits on the martian surface is due to UV photodecomposition in the current environment.     

火星探测着陆系统开伞控制方法研究

文章通过对火星探测进入轨道的分析,提出了一种以动压为控制目标的开伞控制方法,并借鉴过载-时间控制法的相关思想,结合火星探测进入轨道的特点,研究了该开伞控制法的原理和实现方法。根据火星探路者的实际进入条件,采用该方法的分析结果与实际飞行中的开伞情况基本吻合。     

火星探测着陆系统开伞控制方法研究

文章通过对火星探测进入轨道的分析，提出了一种以动压为控制目标的开伞控制方法，并借鉴过载-时间控制法的相关思想，结合火星探测进入轨道的特点，研究了该开伞控制法的原理和实现方法。根据火星探路者的实际进入条件，采用该方法的分析结果与实际飞行中的开伞情况基本吻合。     

火星环境对着陆探测任务的影响及设计考虑

火星着陆探测任务环节多、复杂度高、环境不确定性大,历史成功率低于50％.日前我国首次自主火星探测任务"天问一号"已取得圆满成功,在世界上首次一步实现"绕、落、巡"的火星探测.文章对火星着陆探测任务中考虑的火星环境要素及其量化条件的确定过程进行阐述,包括:探测器系统对环境条件的需求,基于此对火星空间环境、大气环境、表面环...     

Survival of methanogens during desiccation: implications for life on Mars

The relatively recent discoveries that liquid water likely existed on the surface of past Mars and that methane currently exists in the martian atmosphere have fueled the possibility of extant or extinct life on Mars. One possible explanation for the existence of the methane would be the presence of methanogens in the subsurface. Methanogens are microorganisms in the domain Archaea that can metabolize molecular hydrogen as an energy source and carbon dioxide as a carbon source and produce methane. One factor of importance is the arid nature of Mars, at least at the surface. If one is to assume that life exists below the surface, then based on the only example of life that we know, liquid water must be present. Realistically, however, that liquid water may be seasonal just as it is at some locations on our home planet. Here we report on research designed to determine how long certain species of methanogens can survive desiccation on a Mars soil simulant, JSC Mars-1. Methanogenic cells were grown on JSC Mars-1, transferred to a desiccator within a Coy anaerobic environmental chamber, and maintained there for varying time periods. Following removal from the desiccator and rehydration, gas chromatographic measurements of methane indicated survival for varying time periods. Methanosarcina barkeri survived desiccation for 10 days, while Methanobacterium formicicum and Methanothermobacter wolfeii were able to survive for 25 days.