Aquatic modules for bioregenerative life support systems based on the C.E.B.A.S. biotechnology [correction of biotechnilogy

Most concepts for bioregenerative life support systems are based on edible higher land plants which create some problems with growth and seed generation under space conditions. Animal protein production is mostly neglected because of the tremendous waste management problems with tetrapods under reduced weightlessness. Therefore, the "Closed Equilibrated Biological Aquatic System" (C.E.B.A.S.) was developed which represents an artificial aquatic ecosystem containing aquatic organisms which are adapted at all to "near weightlessness conditions" (fishes Xiphophorus helleri, water snails Biomphalaria glabrata, ammonia oxidizing bacteria and the rootless non-gravitropic edible water plant Ceratophyllum demersum). Basically the C.E.B.A.S. consists of 4 subsystems: a ZOOLOGICAL (correction of ZOOLOGICASL) COMPONENT (animal aquarium), a BOTANICAL COMPONENT (aquatic plant bioreactor), a MICROBIAL COMPONENT (bacteria filter) and an ELECTRONICAL COMPONENT (data acquisition and control unit). Superficially, the function principle appears simple: the plants convert light energy into chemical energy via photosynthesis thus producing biomass and oxygen. The animals and microorganisms use the oxygen for respiration and produce the carbon dioxide which is essential for plant photosynthesis. The ammonia ions excreted by the animals are converted by the bacteria to nitrite and then to nitrate ions which serve as a nitrogen source for the plants. Other essential ions derive from biological degradation of animal waste products and dead organic matter. The C.E.B.A.S. exists in 2 basic versions: the original C.E.B.A.S. with a volume of 150 liters and a self-sustaining standing time of more than 13 month and the so-called C.E.B.A.S. MINI MODULE with a volume of about 8.5 liters. In the latter there is no closed food loop by reasons of available space so that animal food has to be provided via an automated feeder. This device was flown already successfully on the STS-89 and STS-90 spaceshuttle missions and the working hypothesis was verified that aquatic organisms are nearly not affected at all by space conditions, i.e. that the plants exhibited biomass production rates identical to the sound controls and that as well the reproductive, and the immune system as the embryonic and ontogenic development of the animals remained undisturbed. Currently the C.E.B.A.S. MINI MODLULE is prepared for a third spaceshuttle flight (STS-107) in spring 2001. Based on the results of the space experiments a series of prototypes of aquatic food production modules for the implementation into BLSS were developed. This paper describes the scientific disposition of the STS-107 experiment and of open and closed aquaculture systems based on another aquatic plant species, the Lemnacean Wolffia arrhiza which is cultured as a vegetable in Southeastern Asia. This plant can be grown in suspension culture and several special bioreactors were developed for this purpose. W. arrhiza reproduces mainly vegetatively by buds but also sexually from time to time and is therefore especially suitable for genetic engineering, too. Therefore it was used, in addition, to optimize the C.E.B.A.S. MINI MODULE to allow experiments with a duration of 4 month in the International Space Station the basic principle of which will be explained. In the context of aquaculture systems for BLSS the continuous replacement of removed fish biomass is an essential demand. Although fish reproduction seems not to be affected in the shortterm space experiments with the C.E.B.A.S. MINI MODULE a functional and reliable hatchery for the production of siblings under reduced weightlessness is connected with some serious problems. Therefore an automated "reproduction module" for the herbivorous fish Tilapia rendalli was developed as a laboratory prototype. It is concluded that aquatic modules of different degrees of complexity can optimize the productivity of BLSS based on higher land plants and that they offer an unique opportunity for the production of animal protein in lunar or planetary bases.     

Novel aquatic modules for bioregenerative life-support systems based on the closed equilibrated biological aquatic system (C.E.B.A.S.)

The closed equilibrated biological aquatic system (C.E.B.A.S) is a man-made aquatic ecosystem which consists of four subcomponents: an aquatic animal habitat, an aquatic plant bioreactor, an ammonia oxidizing bacteria filter and a data acquisition/control unit. It is a precursor for different types of fish and aquatic plant production sites which are disposed for the integration into bioregenerative life-support systems. The results of two successful spaceflights of a miniaturized C.E.B.A.S version (the C.E.B.A.S. MINI MODULE) allow the optimization of aquatic food production systems which are already developed in the ground laboratory and open new aspects for their utilization as aquatic modules in space bioregenerative life support systems. The total disposition offers different stages of complexity of such aquatic modules starting with simple but efficient aquatic plant cultivators which can be implemented into water recycling systems and ending up in combined plant/fish aquaculture in connection with reproduction modules and hydroponics applications for higher land plants. In principle, aquaculture of fishes and/or other aquatic animals edible for humans offers optimal animal protein production under lowered gravity conditions without the tremendous waste management problems connected with tetrapod breeding and maintenance. The paper presents details of conducted experimental work and of future dispositions which demonstrate clearly that aquaculture is an additional possibility to combine efficient and simple food production in space with water recycling utilizing safe and performable biotechnologies. Moreover, it explains how these systems may contribute to more variable diets to fulfill the needs of multicultural crews.     

Animal protein production modules in biological life support systems: novel combined aquaculture techniques based on the Closed Equilibrated Biological Aquatic System (C.E.B.A.S.)

Based on the experiences made with the Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) which was primarily deveoloped for long-term and multi-generation experiments with aquatic animals and plants in a space station highly effective fresh water recycling modules were elaborated utilizing a combination of ammonia oxidizing bacteria filters and higher plants. These exhibit a high effectivity to eliminate phosphate and anorganic nitrogen compounds and arc. in addidition. able to contribute to the oxygen supply of the aquatic animals. The C.E.B.A.S. filter system is able to keep a closed artificial aquatic ecosystem containing teleost fishes and water snails biologically stable for several month and to eliminate waste products deriving from degraded dead fishes without a decrease of the oxygen concentration down to less than 3.5 mg/l at 25 °C. More advanced C.E.B.A.S. filter systems, the BIOCURE filters, were also developed for utilization in semiintensive and intensive aquaculture systems for fishes. In fact such combined animal-plant aquaculture systems represent highly effective productions sites for human food if proper plant and fish species are selected The present papers elucidates ways to novel aquaculture systems in which herbivorous fishes are raised by feeding them with plant biomass produced in the BIOCURE filters and presents the scheme of a modification which utilizes a plant species suitable also for human nutrition. Special attention is paid to the benefits of closed aquaculture system modules which may be integrated into bioregenerative life support systems of a higher complexity for, e. g.. lunar or planetary bases including some psychologiccal aspects of the introduction of animal protein production into plant-based life support systems. Moreover, the basic reproductive biological problems of aquatic animal breeding under reduced gravity are explained leading to a disposition of essential research programs in this context.     

Novel laboratory approaches to multi-purpose aquatic bioregenerative closed-loop food production systems

Based on the construction principle of the Closed Equilibrated Biological Aquatic System (C.E.B.A.S.) two novel combined animal-plant production systems were developed in laboratory scale the first of which is dedicated to mid-term operation in closed state up to two years. In principle both consist of the "classic" C.E.B.A.S. subcomponents: animal tank (Zoological Component), plant cultivators (Botanical Component), ammonia converting bacteria filter (Microbial Component) and data acquisition/control unit (Electronical Component). The innovative approach in the first system is the utilization of minimally three aquatic plant cultivators for different species. In this one the animal tank has a volume of about 160 liters and is constructed as an "endless-way system" surrounding a central unit containing the heat exchanger and the bacteria filter with volumes of about 1.5 liters each. A suspension plant cultivator (1 liter) for the edible duckweed Wolffia arrhiza is externally connected. The second plant cultivator is a meandric microalgal bioreactor for filamentous green algae. The third plant growth facility is a chamber with about 2.5 liters volume for cultivation of the "traditional" C.E.B.A.S. plant species, the rootless buoyant Ceratophyllum demersum. Both latter units are illuminated with 9 W fluorescent lamps. In the current experiment the animal tank contains the live-bearing teleost fish Xiphophorus helleri and the small pulmonate water snail Biomphalaria glabrata because their physiological adaptation to the closed system conditions is well known from many previous C.E.B.A.S. experiments. The water temperature is maintained at 25 degrees C and the oxygen level is regulated between 4 and 7 mg/l by switching on and off the plant cultivator illuminations according to a suitable pattern thus utilizing solely the oxygen produced by photosynthesis. The animals and the microorganisms of filter and biofilm provide the plants with a sufficient amount of carbon dioxide. Oxygen concentration, pH value, temperature and redox potential are on-line recorded. Ion concentrations and numbers of living germs in the system water are determined twice monthly in the laboratory from samples taken from a special "sample removal module"; the sample volume is automatically replaced from an reservoir container. A rotatory pump produces a water flow of about 38 l/min. For a similar smaller test system with approx. 10 l volume developed from the C.E.B.A.S.-MINI-MODULE a novel indirect solar energy supply is tested which has a buffer capacity to maintain the system for 7 days in darkness under central European climate conditions also in winter. It contains only a single plant cultivator which is operated with Wollfia arrhiza. This lemnacean plant is able to produce large amounts of plant biomass in a short time by vegetative reproduction via daughter fronds. This easy-to-handle apparatus is dedicated to be operative more than 4 month. The experimental animals and microorganisms are the same as in the large system. The paper provides detailed information on the system construction principles and the biological, physical and chemical data of the initial phase of the test runs of both systems with the main focus on the large one.     

Perspectives of different type biological life support systems (BLSS) usage in space missions

In the paper an attempt is made to combine three important criteria of LSS comparison: minimum mass, maximum safety and maximum quality of life. Well-known types of BLSS were considered: with higher plant, higher plants and mushrooms, microalgae, and hydrogen-oxidizing bacteria. These BLSSs were compared in terms of "integrated" mass for the case of a vegetarian diet and a "normal" one (with animal proteins and fats). It was shown that the BLSS with higher plants and incineration of wastes becomes the best when the exploitation period is more than 1 yr. The dependence of higher plants' LSS structure on operation time was found. Comparison of BLSSs in terms of integral reliability (this criterion includes mass and quality of life criteria) for a lunar base scenario showed that BLSSs with higher plants are advantageous in reliability and comfort. This comparison was made for achieved level of technology of closing and for perspective one.     

Assessment of the possibility of establishing material cycling in an experimental model of the bio-technical life support system with plant and human wastes included in mass exchange

A pilot model of a bio-technical life support system (BTLSS) including human and plant wastes has been developed at the Institute of Biophysics SB RAS (Krasnoyarsk, Russia). This paper describes the structure of the photosynthesizing unit of the system, which includes wheat, chufa and vegetables. The study substantiates the simultaneous use of neutral and biological substrates for cultivating plants. A novel physicochemical method for the involvement of human wastes in the cycling has been employed, which enables the use of recycled products as nutrients for plants. Inedible plant biomass was subjected to biological combustion in the soil-like substrate (SLS) and was thus involved in the system mass exchange; NaCl contained in native urine was returned to the human through the consumption of Salicornia europaea, an edible salt-concentrating plant. Mass transfer processes in the studied BLSS have been examined for different chemical components.     

Response of Phaseolus vulgaris L. plants to low-let ionizing radiation: Growth and oxidative stress

The scenarios for the long-term habitation of space platforms and planetary stations involve plants as fundamental part of Bioregenerative Life Support Systems (BLSS) to support the crew needs. Several constraints may limit plant growth in space: among them ionizing radiation is recognized to severely affect plant cell at morphological, physiological and biochemical level. In this work, plants of Phaseolus vulgaris L. were subjected to four different doses of X-rays (0.3, 10, 50 and 100 Gy) in order to assess the effects of ionizing radiation on this species and to analyze possible mechanisms carried out to overcome the radiation injuries. The effects of X-rays on plant growth were assessed by measuring stem elongation, number of internodes and leaf dry weight. The integrity of photosynthetic apparatus was evaluated by photosynthetic pigment composition and ribulose 1,5-bisphosphate carboxylase (Rubisco) activity, whereas changes in total antioxidant pool and glutathione S transferase activity (GST) were utilized as markers of oxidative stress. The distribution of phenolic compounds in leaf tissues as natural shielding against radiation was also determined.Irradiation of plants at 0.3 and 10 Gy did not determine differences in all considered parameters as compared to control. On the contrary, at 50 and 100 Gy a reduction of plant growth and a decrease in photosynthetic pigment content, as well as an increase in phenolic compounds and a decrease in total antioxidant content and GST activity were found. Only a slight reduction of Rubisco activity in leaves irradiated at 50 and 100 Gy was found. The overall results indicate P. vulgaris as a species with a good potential to face ionizing radiation and suggest its suitability for utilization in BLSSs.     

Changes in the loco-regional Cerebral Blood Flow (r.C.B.F.) during a simulation of weightlessness.

Experiments of prolonged bedrest in antiorthostatic position are conducted in order to simulated cardio-circulatory modifications observed in weightlessness. Until now, no studies of r.C.B.F. have been effected in these conditions. Six young, healthy volunteers (average age 23.8) were placed in strict bedrest and in antiorthostatic position -4 degrees for 7 days. The r.C.B.F. measurements were studied by 133Xe inhalation method using a 32 detectors system. Studies were made first in basal conditions, then between the 6th and 12th hr, and finally between the 72nd and the 78th hr after the beginning of the experiment. Three of the subjects received 0.450 mg of Clonidine daily during the experiment. In the subjects having taken no Clonidine, we observed a constant increase in r.C.B.F. (12, 17 and 16% respectively) in the first 12 hr; at the 72nd hour, all values had returned to basal state. This findings agrees with the well known notion of a rapid correction of hemodynamic disturbances observed in the first days of weightlessness. In the subjects treated with Clonidine, the increase of r.C.B.F. did not occur. Several mechanisms of action are possible; the Clonidine affecting either the heart by inhibiting volemic atrial receptors or the brain by direct vasoconstriction.     

Meeting the needs of the new millennium: passenger space travel and world economic growth

Without doubt, humans’ most urgent need at the start of the new millennium is the continuation of economic growth, which is the only means by which the great majority of the world population can lift themselves out of the poverty in which they live. A sine qua non for continuing economic growth is for the rich countries to continue to develop new industries—as they did throughout the 20th century, thereby creating high-productivity employment for hundreds of millions of people around the world. Arguably the most significant of these thus far is the development of passenger air travel from zero in 1900 to 1.5 billion passengers per year by 2000. It is becoming clear that passenger space travel could grow to reach a similar economic scale—and that no other space activity has comparable potential. The paper describes the potential contribution to world economic growth of passenger space travel; the failure of government space agencies either to aid its development or to make a contribution to economic growth commensurate to their cost; and the value for economic policy of prioritising the realisation of passenger space travel. The faster passenger space travel services grow, the more the space industry will contribute to “Meeting the Needs of the New Millennium”.     

A socio-economic evaluation of the lunar environment and resources: II. Energy for the selenosphere

This first of several study papers, based on a fundamental paper presented in 1972, provides an independent conceptual analysis and evaluation of the lunar environment as industrial base and habitat. A selenosphere system strategy is outlined. The underlying concept is that of one or several lunar industrial zones for resource extraction and on-surface processing, integrated with a circumlunar zero-g processing capability, serving markets in geolunar space. A classification of lunar elements by utilization category is presented. Lunar oxygen is a prime candidate for being an initial economic “drawing card”, because of its value for fast transportation in geolunar space, requiring significantly fewer ships for equal transfer capability per unit time than electric transports which, however, have value, especially between geosynchronous and lunar orbit. The reduced development difficulties of controlled fusion outside the atmosphere and its advantages for extracting oxygen and other elements in quantity are summarized. Examples of lunar cycle management as fundamental exoindustrial requirement for economic resource enhancement are presented. The principal initial socio-economic value of lunar industry lies in the use of lunar resources for exoindustrial products and operations designed to accelerate, intensify and diversify Earth-related benefits. In the longer run, lunar settlements are a highly suitable proving ground for studying and testing the complex matrix of technological, biological, cultural, social and psychological aspects that must be understood and manageable before large settlements beyond Earth can have a realistic basis for viability. The lunar environment is more suitable for experimentation and comparatively more “forgiving” in case of failures than is orbital space.     

Life and intelligence in the universe from nanobiological principles: A survey and budget of concepts and perspectives

The new-born bioscience called Nanobiology has tackled the problems of the possibility of existence of extraterrestrial life and intelligence and of biosystem distribution in the Universe, as such questions actually belong to the realm of Theoretical Biology. The central, and yet unanswered points of such science have been reinvestigated by attempting knowledge and control of the hard-to-determine nanoscale-level classical and quantum interactions, which would supposedly give mechanistic, definite answers, both informationally and energetically, to the vexing questions put by biosystems to science: is the “living state” a physically definible concept, and how to define it? Are nanoscale kinetics or even detailed mechanics involved in the origin of life? What about intelligence, consciousness and their nanophysical roots? Are “life” and “intelligence” engineerable properties, or is any Artificial Intelligence program bound to mere metaphors? Self-organization, studied at the thermodynamic and the hydrodynamic level, showed the possibility of chemical evolution from amino acids, probably of cometary and/or meteoritic origin, up to spatiotemporal organization, autopoiesis and biological evolution, but didn't explain the origins of life. Questioning the uniqueness of the earthly evolutionary chemistry is cardinal for the ETI dilemma, as from a budgetary appraisal of perspectives in bionanoscale chaotic undecidable dynamics, quantum gravity and quantum vacuum, both “living state” and “intelligence” look like nonlocal, spacetime-linked cosmic phenomena.     

SETI via Wormholes

The special theory of relativity rests on the assumption that in no case can the speed of light be exceeded. Rather surprisingly, however, recent advances in the general theory of relativity show that Faster-Than-Light (FTL) travel is allowed by Einstein’s gravitational theory. An explanation of this apparent contrast between special and general relativity lies in the fact that general relativity uses non-linear differential equations and non-Euclidean spacetime geometry that special relativity does not. Therefore, this larger mathematical armoury makes room for a whole new class of very subtle and unexpected relativistic phenomena to come to light. One of these is the Theory of Wormholes, more politely termed Tunnels into Space–Time. In 1988, Kip S. Thorne and Michael S. Morris published a path-breaking paper about Wormholes showing how spaceflight between two stars might be possible in a time of hours if a “tunnel” dug into space–time exists between them. However, they also showed that keeping the tunnel open for the spaceship to travel through would require a kind of matter, called “exotic” by them, that does not appear to exist in nature, because its tensional strength would have to exceed the energy density of its matter. This request is a severe constraint to the natural existence of Morris–Thorne Wormholes, or even to their artificial construction by an advanced civilization. In 1995, however, the present author sought to replace the exotic matter in a Morris–Thorne Wormhole by a very intense magnetic field. Such “Magnetic Wormholes” could indeed exist because very intense magnetic fields are already known to exist on the surface of neutron stars and pulsars. This paper discusses the consequences on SETI of the possible existence of Magnetic Wormholes. Phenomena of divergent gravitational lensing might possibly occur in the proximity of pulsars and neutron stars. These effects could help us detect signals from very far civilisations by virtue of ordinary SETI techniques already in use.     

The famous son of Ukrainian people V.I. Voznyuk who has provided launch of all ballistic missiles of the cosmodrome Kapustin Yar

The history of the life of V.I. Voznyuk is a history of the phenomenon of the Soviet rocket progress when the engineers with experience of launch of military rocket of small radius of action were testing the ballistic missiles. The remarkable and little-known destiny of Voznuk is the history of the Soviet rocket technology experts who had a severe practical schooling of command by the military forces of the first combat missiles “Katucha” during the grim military years (including the grandiose fight in Stalingrad) and then they have continued to launch the ballistic missiles. V.I. Voznyuk worked as the chief of the first Soviet cosmodrome Kapustin Yar for almost 30 years—since the most difficult moment of its organization. He organized a launch of the first Soviet ballistic missiles R-1, R-2, R-5M of S. Korolev. This report is about the outstanding achievement of the organizing ability of V.I. Voznyuk—about the launch of a missile with a nuclear warhead in 1956. V.I. Voznyuk closes a unique chain in the world of outstanding figures of space-rocket technology who were born or lived in Ukraine from designers of missile up to the organizers of its manufacture and now up to the organizers of the tests of rockets—J. Aizenberg, V. Budnik, O. Baclanov, V. Dogujiev, M. Galasj, N. Gerasuta, V. Gluschko, B. Gubanov, A. Gudimenko, I. Ivanov, G. Kesunjko, B. Konoplev, S. Korolev, V. Kovtunenko, V. Kukuschkin, O. Makarov, A. Nedaivoda, M. Reshetniyov, Yu. Semenov, V. Sergeev, Yu. Smetanin, V. Tchelomey, D. Torchiy, V. Utkin and M. Yangel.     

Summary of medical investigations in the U.S.S.R. manned space missions.

The results of biomedical investigations carried out in the U.S.S.R. manned space missions are discussed. Their basic result is well-documented evidence that man can perform space flights of long duration. The investigations have demonstrated no direct correlation between inflight or postflight physiological reactions of crewmembers and flight duration. In all likelihood, this can be attributed to the fact that special exercises done inflight efficiently prevented adverse effects of weightlessness. However, human reactions to weightlessness need further study. They include negative calcium balance and anemia as well as vestibulo-autonomic disorders shown by crewmembers at early stages of weightlessness. Attention should be given to psychological, social-psychological and ethical problems that may also limit further increase in flight duration.     

U.S. prebreathe protocol

This paper identifies and describes the prebreathe protocol currently used by the U.S. Space Shuttle Program to provide astronauts the capability to safely perform extravehicular activity. A comparison of planned vs actual prebreathe experience through the STS-37 Mission is also provided.     

The overredundant spacecraft—A competitive approach to future telecommunication satellites

Present operational space telecommunication systems are based on simultaneous availability of more than one satellite on orbit, mainly a spare satellite in addition to the operational one.Considering the costs associated to the delivery of extra flight models and to extra launchers, the question is asked whether it would not be advantageous to launch a very limited number of “overredundant” spacecraft instead of several standard satellites.The paper gives main conditions of reliability, size and redundancy concept under which an “overredundant” spacecraft could be a competitive approach to future operational systems.     

X-38, CRV and CRV Evolution Program Overview and European Role

The X-38 Project forms part of the “X” prototype vehicle family developed by the United States. Its development was initiated by NASA to prepare the Crew Return Vehicle (CRV). The European participation in the X-38 Program has been significantly extended since the start of the X-38 cooperation in 1997 and is realized by ESA's “Applied Reentry Technology Program” and the German/DLR “Technologies for Future Space Transportation Systems” (TETRA) Project. European contributions to the X-38 Vehicle 201, (V-201) can be found in all technical key areas. The orbital flight and reentry with the X-38 V-201 will conclude the X-38 project in 2002.The CRV will be used from about mid-2005 as ’ambulance‘, ’lifeboat‘ or as alternate return vehicle for the crew of the International Space Station. Recognizing the very productive and mutually beneficial cooperation established on X-38, NASA and ESA have decided to continue this cooperation into the development of the operational CRV. The Phase C/D will be completed shortly after the Critical Design Review, scheduled for August 2002. The CRV production phase will start in October 2002 and will cover production of four CRV vehicles, ending in 2006.Based on the objective to identify a further evolution potential of the CRV towards a Crew Cargo Transfer Vehicle (CCTV), NASA has implemented upgrade studies in the CRV Phase C/D.     

Natriuretic peptide-sensitive guanylyl cyclase expression is down-regulated in human melanoma cells at simulated weightlessness

The membrane-bound guanylyl cyclases A and B (GC-A/B), which are receptors for natriuretic peptides, are expressed in cancer cells including melanomas and may represent new anticancer targets. Here, we report down-regulation of GC-A/B expression in human metastatic melanoma cells at simulated weightlessness in comparison to 1g conditions, suggesting attenuation of metastatic potential in weightlessness.     

The case for small space missions

Three orders of arguments are outlined to illustrate the importance of small missions. First, the classical reasons (one has to begin small, educational aspects, introduction to the space field) are summarized; then some thoughts are introduced on the efficacy and the efficiency of the effort invested (how expensive is a national project, and how advantageous the inevitable multinational cooperation); finally, a synopsis of the considerations about the need for cultural changes in the space program (the challenge of the coming era, the resulting needs, the philosophy of “small missions” and its contributions) is given.