Survival and germinability of Bacillus subtilis spores exposed to simulated Mars solar radiation: implications for life detection and planetary protection

Bacterial spores have been considered as microbial life that could survive interplanetary transport by natural impact processes or human spaceflight activity. Deposition of terrestrial microbes or their biosignature molecules onto the surface of Mars could negatively impact life detection experiments and planetary protection measures. Simulated Mars solar radiation, particularly the ultraviolet component, has been shown to reduce spore viability, but its effect on spore germination and resulting production of biosignature molecules has not been explored. We examined the survival and germinability of Bacillus subtilis spores exposed to simulated martian conditions that include solar radiation. Spores of B. subtilis that contain luciferase resulting from expression of an sspB-luxAB gene fusion were deposited on aluminum coupons to simulate deposition on spacecraft surfaces and exposed to simulated Mars atmosphere and solar radiation. The equivalent of 42 min of simulated Mars solar radiation exposure reduced spore viability by nearly 3 logs, while germination-induced bioluminescence, a measure of germination metabolism, was reduced by less than 1 log. The data indicate that spores can retain the potential to initiate germination-associated metabolic processes and produce biological signature molecules after being rendered nonviable by exposure to Mars solar radiation.     

The biological effectiveness of HZE-particles of cosmic radiation studied in the Apollo 16 and 17 Biostack experiments

The Biostack experiments I and II were flown on board the Apollo 16 and 17 command modules in order to obtain information on the biological damage produced by the bombardment of heavy high-energy (HZE) particles of cosmic radiation during spaceflight. Such data are required for estimating radiation hazards in manned spaceflight. Seven biological systems in resting state (Bacillus subtilis spores, Colpoda cucullus cysts, Arabidopsis thaliana seeds, and eggs of Artemia salina, Tribolium castaneum and of Carausius morosus) were accommodated in the two Biostacks. By using a special sandwich construction of visual track detectors and layers of biological objects, identification of each hit biological object was achieved and the possible biological damage correlated with the physical features of the responsible HZE-particle. In the different systems the degree of damage depended on whether the hit cell was replaceable or not. A high sensitivity to HZE-particle bombardment was observed on Artemia salina eggs; 90% of the embryos, which were induced to develop from hit eggs, died at different developmental stages. Malformations of the abdomen or the extremities of the nauplius were frequently induced. In contrast, the growth of hit Vicia faba radiculae and the germination of hit Arabidopsis thaliana seeds and hit Bacillus subtilis spores were not influenced remarkably. But there was an increase in multicaulous plants and a reduction in the outgrowth of the bacterial spores. In addition, information was obtained on the fluence of the HZE-particles, on their spectrum of charge and energy loss, and on the absorption by the Apollo spacecraft and the Biostack material itself. This will help to improve knowledge concerning radiation conditions inside of spacecrafts, necessary to secure a maximum possible protection to the astronauts.     

Response and adaptation of bone cells to simulated microgravity

Bone loss induced by microgravity during space flight is one of the most deleterious factors on astronaut’s health and is mainly attributed to an unbalance in the process of bone remodeling. Studies from the space microgravity have demonstrated that the disruption of bone remodeling is associated with the changes of four main functional bone cells, including osteoblast, osteoclast, osteocyte, and mesenchymal stem cells. For the limited availability, expensive costs and confined experiment conditions for conducting space microgravity studies, the mechanism of bone cells response and adaptation to microgravity is still unclear. Therefore, some ground-based simulated microgravity methods have been developed to investigate the bioeffects of microgravity and the mechanisms. Here, based on our studies and others, we review how bone cells (osteoblasts, osteoclasts, osteocytes and mesenchymal stem cells) respond and adapt to simulated microgravity.     

Using a mini-Raman spectrometer to monitor the adaptive strategies of extremophile colonizers in arid deserts: relationships between signal strength, adaptive strategies, solar radiation, and humidity

Abstract The survival strategies of one cyanobacteria colony and three terricolous lichen species from the hot subdesert of Tabernas, Spain, were studied along with topographical attributes of the area to investigate whether the protective strategies adopted by these pioneer soil colonizers are related to the environmental stressors under which they survive. A handheld Raman spectrometer was used for biomolecular characterization, while the microclimatic and topographic parameters were estimated with a Geographic Information System (GIS). We found that the survival strategies adopted by those organisms are based on different combinations of protective biomolecules, each with diverse ecophysiological functions, such as UV-radiation screening, free-energy quenching, antioxidants, and the production of different types and amounts of calcium oxalates. Our results show that the cyanobacteria community and each lichen species preferentially colonized a particular microhabitat with specific moisture and incident solar radiation levels and exhibited different adaptive mechanisms. In recent years, a number of studies have provided consistent results that suggest a link between the strategies adopted by those extremophile organisms and the microclimatic environmental parameters. To date, however, far too little attention has been paid to results from Raman analyses on dry specimens. Therefore, the results of the present study, produced with the use of our miniaturized instrument, will be of interest to future studies in astrobiology, especially due to the likely use of Raman spectroscopy at the surface of Mars. Key Words: Hot desert-Raman spectroscopy-Topography-Terricolous lichens-Cyanobacteria-Planetary exploration. Astrobiology 12, 743-753.     

Time delays in the nonthermal radiation of solar flares according to observations of the <Emphasis Type="Italic">CORONAS-F</Emphasis> satellite

In 2001–2003, the X-ray and microwave observations of ten solar flares of M- and X-classes were carried out by the CORONAS-F orbital station, the RSTN Sun service, and Nobeyama radio polarimeters. Based on these observations, a correlation analysis of time profiles of nonthermal radiation was performed. On average, hard X-ray radiation outstrips the microwave radiation in 9 events, i.e., time delays are positive. The appearance of negative delays is associated with effective scattering of accelerated electrons in pitch angles, where the length of the free path of a particle is less than the half-length of a flare loop. The additional indications are obtained in favor of the need to account for the effect of magnetic mirrors on the dynamics of energetic particles in the coronal arches.     

Intermittency of solar wind density fluctuations and its relation to sharp density changes

The paper presents the study of turbulent properties of the solar wind plasma, namely, the intermittency of fluctuations of the solar wind ion flux in the earlier unexplored region of comparatively high frequencies (0.01–1 Hz). Special attention is given to a comparison of intermittency for solar wind observation intervals containing sharp (shorter than 10 min) and high-amplitude (greater than 20%) changes of the ion flux to intervals without such changes. The solar wind observation intervals containing sharp changes of the flux are found to be essentially more intermittent than the intervals of quiet solar wind. Such a comparison allows one to reveal the fundamental difference in turbulent properties of the solar wind depending on the presence or absence of sharp boundaries in plasma structures.     

UV photochemistry of DNA in vitro and in Bacillus subtilis spores at earth-ambient and low atmospheric pressure: implications for spore survival on other planets or moons in the solar system

Two major parameters influencing the survival of Bacillus subtilis spores in space and on bodies within the Solar System are UV radiation and vacuum, both of which induce inactivating damage to DNA. To date, however, spore survival and DNA photochemistry have been explored only at the extremes of Earth-normal atmospheric pressure (101.3 kPa) and at simulated space vacuum (10(-3)-10(-6) Pa). In this study, wild-type spores, mutant spores lacking alpha/beta-type small, acid-soluble spore proteins (SASP), naked DNA, and complexes between SASP SspC and DNA were exposed simultaneously to UV (254 nm) at intermediate pressure (1-2 Pa), and the UV photoproducts cis,syn-thymine-thymine cyclobutane dimer (c,sTT), trans,syn-thymine-thymine cyclobutane dimer (t,sTT), and "spore photoproduct" (SP) were quantified. At 101.3 kPa, UV-treated wild-type spores accumulated only SP, but spores treated with UV radiation at 1-2 Pa exhibited a spectrum of DNA damage similar to that of spores treated at 10(-6) Pa, with accumulation of SP, c,sTT, and t,sTT. The presence or absence of alpha/beta-type SASP in spores was partly responsible for the shift observed between levels of SP and c,sTT, but not t,sTT. The changes observed in spore DNA photochemistry at 1-2 Pa in vivo were not reproduced by irradiation of naked DNA or SspC:DNA complexes in vitro, suggesting that factors other than SASP are involved in spore DNA photochemistry at low pressure.     

某天线支承筒阻尼减振设计与分析

为减小某卫星天线支承筒的振动,根据阻尼减振原理,采用约束阻尼层方法进行减振处理。以ZN-1丁基橡胶为粘性阻尼材料,T700S层合板为约束层,在约束层厚0.4 mm,铺层数为4的条件下,设计了三种不同铺层角的减振方案。有限元计算结果表明,在天线支承筒上附加约束阻尼层可明显降低支承筒指定点处的频率响应。在给定条件下,给出的三种方案均满足工程要求,其中以铺层角45°/-45°/0°/90°方案的效果最佳。     

从对《安妮日记》的改写看奈达的读者反应论

文章基于"Translation:Ideology——On the Construction of Different Anne Franks"一文中关于《安妮日记》不同版本改写,删减的问题,举例说明了读者反应论在跨文化翻译中的作用。     

The heliospheric magnetic field and its relation to the temperature,density, and velocity of solar plasma: Experimental evidence

Using daily and hourly data on solar plasma parameters at the Ulysses spacecraft orbit and at 1 AU it is demonstrated that there is a simple relationship between plasma temperature and density with the heliospheric magnetic field (HMF). A mathematical expression connecting HMF with plasma temperature and density is suggested. Correlation coefficients and regression equations for measured and calculated magnetic fields are presented for the 1990–2009 period according to Ulysses spacecraft data and for 2003–2010 at 1 AU (OMNI database). The roles played by density, temperature, and high-speed solar wind streams in forming the magnetic-field peaks are demonstrated using hourly data of OMNI2 and Ulysses.     

Sporadic structures and small-scale irregularity in the nighttime polar ionosphere in the period of high solar activity according to the data of radio occultation measurements on satellite-to-satellite paths

Results of the analysis of 327 sessions of radio occultation on satellite-to-satellite paths are presented. The data are taken in the nighttime polar ionosphere in the regions with latitudes of 67°–88°, and in the period of high solar activity from October 26, 2003 to November 9, 2003. Typical ionospheric changes in the amplitude and phase of decimeter radio waves on paths GPS satellites-CHAMP satellite are presented. It is demonstrated that these data make it possible to determine characteristics of the sporadic E _s structures in the lower ionosphere at heights of 75–120 km. Histograms of distribution of the lower and upper boundaries, thickness, and intensity of the E _s structures are presented. Dispersion and spectra of amplitude fluctuations of decimeter radio waves caused by small-scale irregularity of the ionospheric plasma are analyzed. The relation of the polar E _s structures and intensity of small-scale plasma irregularity to various manifestations of solar activity is discussed. The efficiency of monitoring the ionospheric disturbances caused by shock waves of the solar wind by the radio occultation method on satellite-to-satellite paths is demonstrated.