Planetary protection and the search for life beneath the surface of Mars.

The search for traces of extinct and extant life on Mars will be extended to beneath the surface of the planet. Current data from Mars missions suggesting the presence of liquid water early in Mars' history and mathematical modeling of the fate of water on Mars imply that liquid water may exist deep beneath the surface of Mars. This leads to the hypothesis that life may exist deep beneath the Martian surface. One possible scenario to look for life on Mars involves a series of unmanned missions culminating with a manned mission drilling deep into the Martian subsurface (approximately 3Km), collecting samples, and conducting preliminary analyses to select samples for return to earth. This mission must address both forward and back contamination issues, and falls under planetary protection category V. Planetary protection issues to be addressed include provisions stating that the inevitable deposition of earth microbes by humans should be minimized and localized, and that earth microbes and organic material must not contaminate the Martian subsurface. This requires that the drilling equipment be sterilized prior to use. Further, the collection, containment and retrieval of the sample must be conducted such that the crew is protected and that any materials returning to earth are contained (i.e., physically and biologically isolated) and the chain of connection with Mars is broken.     

Sterilisation properties of the Mars surface and atmospheric environment.

The radiative and chemical conditions at the surface and in the lower Martian atmosphere are computed at various latitudes and seasons combining a 2D photochemical model and radiation simulations. In most situations, the solar UV B and C radiations reach the surface however, suspended dust and, in polar cases, ozone can constitute an effective UV shield. The daytime and night time concentrations of the sterilizing oxidants: OH, H2O2 and O3 are determined, as well as the concentration of the substances which could influence the metabolism of microorganisms. The possible habitats of a remaining Mar's life as well as the possibilities of contamination by resistant earth life forms will be described.     

Mars glob: Creation of an international network of Mars surface landers

Program MARS GLOB provides step-by-step deployment of an international network of Mars surface stations by association the MESUR NETWORK (USA), INTERMARS-NET (ESA) programs with the network of small stations and penetrators now under developing in Russia jointly with international cooperation in frameworks of the MARS-96 Project. It is offering also delivery on Mars surface two penetrators and Mars Rover. Now penetrators and Rover are developing by Russia with participation of other countries in frameworks of the MARS-98 (or MARS TOUR) Project.     

Application of ISFETs in closed-loop systems for greenhouses.

In horticulture, growing in artificial substrates such as rockwool is more and more considered to be a sound alternative to growing in soil. This development enables the opportunity to create closed-loop systems which lower the waste of raw materials and reduce pollution of the environment. Applying closed-loop systems needs precise knowledge of the composition of the recirculating nutrient solution. This paper presents basic principles of a measuring system, which can monitor continuously the concentration of nutrients in water. The system is based on ion-selective field effect transistors (ISFETs). By appropriate calibration, a high accuracy is achieved for pH and potassium measurements in the nutrient solution. An accuracy of better than 10% (mMol/l) has been achieved.     

Peroxides and the survivability of microorganisms on the surface of Mars.

Results of the Viking mission seem to indicate that there is a ubiquitous layer of highly oxidizing aeolian material covering the Martian surface. This layer is thought to oxidize organic material that may settle on it, and is therefore responsible for the lack of detection of organic matter on the planet's surface by Viking. The mechanism that creates the oxidizing condition is not well understood, nor is the extent of the oxidation potential of this material. It has been suggested that the oxidizing nature of the soil is due to photochemical reactions which create hydrogen peroxide and superoxides in the surface soil. One question of importance to planetary protection regarding this material is, what is its potential for destroying terrestrial microorganisms, thus making the surface of Mars "self-sterilizing"? Using data obtained by the gas exchange experiment on Viking, and for simplicity assuming that all of the O2 released came from H2O2, the concentration range for H2O2 on the surface of Mars can be calculated to be 25-250 ppm. The microbial disinfection rate by H2O2 is concentration dependent, and is highly variable within the microbial community. Data from our laboratory indicate that certain soil bacteria survive and grow to stationary phase in 30,000 ppm H2O2. However, the total number of organisms decreases in the presence of H2O2. These results indicate that it is doubtful that the presence of H2O2 alone on Mars would make the surface "self-sterilizing".     

Life support approaches for Mars missions.

Life support approaches for Mars missions are evaluated using an equivalent system mass (ESM) approach, in which all significant costs are converted into mass units. The best approach, as defined by the lowest mission ESM, depends on several mission parameters, notably duration, environment and consequent infrastructure costs, and crew size, as well as the characteristics of the technologies which are available. Generally, for the missions under consideration, physicochemical regeneration is most cost effective. However, bioregeneration is likely to be of use for producing salad crops for any mission, for producing staple crops for medium duration missions, and for most food, air and water regeneration for long missions (durations of a decade). Potential applications of in situ resource utilization need to be considered further.     

Life support systems for Mars transit.

The long-held human dream of travel to the stars and planets will probably be realized within the next quarter century. Preliminary analyses by U.S. scientists and engineers suggests that a first trip to Mars could begin as early as 2016. A proposal by U.S.S.R. space planners has suggested that an effort involving the cooperation and collaboration of many nations could begin by 2011. Among the major considerations that must be made in preparation for such an excursion are solidification of the scientific, economic and philosophical rationales for such a trip made by humans, and realistic evaluations of current and projected technical capabilities. Issues in the latter category include launch and propulsion systems, long term system stability and reliability, the psychological and physiological consequences of long term exposure to the space environment, the development and use of countermeasures to deleterious human physiological responses to the space environment, and life support systems that are both capable of the immense journey and reliable enough to assure their continued operation for the duration of the voyage. Many of the issues important in the design of a life support system for a Mars trip are based on reasonably well understood data: the human requirements for food, oxygen and water. However, other issues are less well-defined, such as the demands that will be made on the system for personal cleanliness and hygiene, environmental cleanliness, prevention or reduction of environmental toxins, and psychological responses to the environment and to the diet. It is much too early to make final decisions about the characteristics of the long-duration life support system needed for travel to Mars, or for use on its surface. However, it is clear that life support systems will evolve during the next few decades form the relatively straightforward systems that are used on Shuttle and Soyuz, to increasingly more complex and regenerative systems. The Soviet Union has an operating life support system on Mir that can apparently evolve, and the United States is currently planning the one for Space Station Freedom that will use partial regeneration. It is essential to develop concepts now for life support systems on an advanced Space Station, the lunar outpost (to be launched in about 2004) and the lunar base. Such concepts will build on current technology and capabilities. But because of the variety of different technologies that can be developed, and the potential for coordinating the functions of very diverse sub-systems within the same life support system, the possibility of developing an efficient, reliable mixed process system is high. It is likely that a life support system for Mars transit and base will use a composite of physical, chemical, and biological processes. The purpose of this paper is to explore the potentially useful structural elements of a life support system for use on a Mars trip, and to identify the features that, at this time, appear to be most appropriate for inclusion in the system.     

Waste system implications for Mars missions.

Waste technologies for Mars missions have been analyzed, considering equivalent system mass and interface loads. Storage or dumping seems most appropriate for early missions with low food closure. Composting or other treatment of inedible biomass in a bioreactor seems most attractive for moderate food closure (50-75%). Some form of physicochemical oxidation of the composted residue might be needed for increased food closure, but oxidation of all waste does not seem appropriate due to excess of production of carbon dioxide over demand. More comprehensive analysis considering interfaces with other mission systems is needed. In particular, in-situ resource utilization is not considered, and might provide resources more cheaply than waste processing.     

Radiation issues for piloted Mars mission.

Man is now entering an era of colonizing the moon and exploration of Mars. The crewmembers of a piloted mission to Mars will be exposed to inner belt trapped protons, the outer trapped electrons, and the galactic cosmic radiation. In addition there is always the added risk of acute exposure to a solar particle event. Current radiation risk is estimated using the idea of absorbed dose and ICRP-26, LET-dependent quality factors. In a spacecraft with aluminum walls (2 g cm-2) at solar minimum the calculated dose equivalent is 0.73 Sv for a 406-day mission. Based on the current thinking this leads to an excess cancer mortality in a 35 year male of about 1%. About 75% of the dose equivalent is contributed by HZE particles and target fragments with average quality factors of 10.3 and 20, respectively. The entire concept of absorbed dose, quality factor, and dose equivalent as applied to such missions needs to be reexamined, in light of the fact that less than 50% of the nuclei in the body of the astronaut would have been traversed by a single GCR nuclei in the 406-day mission. Clearly, more biologically relevant information about the effects of heavy ions and target fragments is needed and fluence based risk estimation strategy developed for such long term stays in space.     

Remote sensing for organics on Mars.

The detection of organics on Mars remains an important scientific objective. Advances in instrumentation and laboratory techniques provide new insight into the lower level detection limit of complex organics in closely packed media. Preliminary results demonstrate that algae present in a palagonite medium do exhibit a spectral reflectance feature in the visible range for dry mass weight ratios of algae to palagonite greater than 6%--which corresponds to 30 mg algae in a 470 mg (just optically thick (< 3 mm) layer) palagonite matrix. This signature most probably represents chlorophyll a, a light harvesting pigment with an emission peak at 678 nm.     

Biological life-support systems for Mars mission.

Mars mission like the Lunar base is the first venture to maintain human life beyond earth biosphere. So far, all manned space missions including the longest ones used stocked reserves and can not be considered egress from biosphere. Conventional path proposed by technology for Martian mission LSS is to use physical-chemical approaches proved by the experience of astronautics. But the problem of man living beyond the limits of the earth biosphere can be fundamentally solved by making a closed ecosystem for him. The choice optimum for a Mars mission LSS can be substantiated by comparing the merits and demerits of physical-chemical and biological principles without ruling out possible compromise between them. The work gives comparative analysis of ecological and physical-chemical principles for LSS. Taking into consideration universal significance of ecological problems with artificial LSS as a particular case of their solution, complexity and high cost of large-scale experiments with manned LSS, it would be expedient for these works to have the status of an International Program open to be joined. A program of making artificial biospheres based on preceding experience and analysis of current situation is proposed.     

MEP (Mars Environment Package): toward a package for studying environmental conditions at the surface of Mars from future lander/rover missions.

In view to prepare Mars human exploration, it is necessary to promote and lead, at the international level, a highly interdisciplinary program, involving specialists of geochemistry, geophysics, atmospheric science, space weather, and biology. The goal of this program will be to elaborate concepts of individual instruments, then of integrated instrumental packages, able to collect exhaustive data sets of environmental parameters from future landers and rovers of Mars, and to favour the conditions of their implementation. Such a program is one of the most urgent need for preparing human exploration, in order to develop mitigation strategies aimed at ensuring the safety of human explorers, and minimizing risk for surface operations. A few main areas of investigation may be listed: particle and radiation environment, chemical composition of atmosphere, meteorology, chemical composition of dust, surface and subsurface material, water in the subsurface, physical properties of the soil, search for an hypothesized microbial activity, characterization of radio-electric properties of the Martian ionosphere. Scientists at the origin of the present paper, already involved at a high degree of responsibility in several Mars missions, and actively preparing in situ instrumentation for future landed platforms (Netlander--now cancelled, MSL-09), express their readiness to participate in both ESA/AURORA and NASA programs of Mars human exploration. They think that the formation of a Mars Environment working group at ESA, in the course of the AURORA definition phase, could act positively in favour of the program, by increasing its scientific cross-section and making it still more focused on human exploration.     

Science strategy for human exploration of Mars.

The scientific objectives of Mars exploration can be framed within the overarching theme of exploring Mars as another home for life, both for evidence of past or present life on Mars, and as a potential future home for human life. The two major areas of research within this theme are: 1) determining the relationship between planetary evolution, climate change, and life, and 2) determining the habitability of Mars. Within this framework, this paper discusses the exploration objectives for exobiology, climatology and atmospheric science, geology, and martian resource assessment. Human exploration will proceed in four major phases: 1) Precursor missions which will obtain environmental knowledge necessary for human exploration, 2) Emplacement phase which includes the first few human landings where crews will explore the local area of the landing site; 3) Consolidation phase missions where a permanent base will be constructed and crews will be capable of detailed exploration over regional scales; 4) Utilization phase, in which a continuously occupied permanent Mars base exists and humans will be capable of detailed global exploration of the martian surface. The phases of exploration differ primarily in the range and capabilities of human mobility. In the emplacement phase, an unpressurized rover, similar to the Apollo lunar rover, will be used and will have a range of a few tens of kilometers. In the Consolidation phase, mobility will be via a pressurized all-terrain vehicle capable of expeditions from the base site of several weeks duration. In the Utilization phase, humans will be capable of several months long expeditions to any point on the surface of Mars using a suborbital rocket equipped with habitat, lab, and return vehicle. Because of human mobility limitations, it is important to extend the range and duration of exploration in all phases by using teleoperated rover vehicles. Site selection for human missions to Mars must consider the multi-decade time frame of these four phases. We suggest that operations in the first two phases be focused in the regional area containing the Coprates Quadrangle and adjacent areas.     

Legal aspects of planetary protection for Mars missions.

The planning and execution of manned and robotic missions to Mars present a wide range of jurisprudential issues. Provisions to prevent the disruption of natural celestial environments, as well as damage to the environment of Earth by the return of extraterrestrial materials, are important components of the law applicable to mankind's activities in outer space, and have been supplemented by scientifically instituted planetary protection policies. However, divergent legal regimes may exist, as the space treaties in force are neither uniform in their provisions, nor identical as to the states which have signed, ratified, or adopted the international agreements. The legal requirements applicable to a specific mission will vary depending on the entities conducting the program and specific mission profile. This article analyzes the divergent international legal regimes together with the factors which will influence the determination of the standards of conduct which will govern manned and robotic missions to Mars.     

Radiation environment monitoring for manned missions to Mars.

In this paper a radiation monitoring system for manned Mars missions is described, based on the most recent requirements on crew radiation safety. A comparison is shown between the radiation monitoring systems for Earth-orbiting and interplanetary spacecraft, with similarities and differences pointed out and discussed. An operational and technological sketch of the chosen problem solving approach is also given.     

Studies in the search for life on Mars.

The ability of living organisms to survive extraterrestrial conditions has implications for the origins of life in the solar system. We have therefore studied the survival of viruses, bacteria, yeast, and fungi under simulated Martian conditions. The environment on Mars was simulated by low temperature, proton irradiation, ultraviolet irradiation, and simulated Martian atmosphere (CO2 95.46%, N2 2.7%, water vapor 0.03%) in a special cryostat. After exposure to these conditions, tobacco mosaic virus and spores of Bacillus, Aspergillus, Clostridium, and some species of coccus showed significant survival.     

Exobiological exploration of Mars.

Of all the other planets in the solar system, Mars remains the most promising for further elucidating concepts about chemical evolution and the origin of life. Strategies were developed to pursue three exobiological objectives for Mars exploration: determining the abundance and distribution of the biogenic elements and organic compounds, detecting evidence of an ancient biota on Mars, and determining whether indigenous organisms exist anywhere on the planet. The three strategies are quite similar and, in fact, share the same sequence of phases. In the first phase, each requires global reconnaissance and remote sensing by orbiters to select sites of interest for detailed in situ analyses. In the second phase, lander missions are conducted to characterize the chemical and physical properties of the selected sites. The third phase involves conducting "critical" experiments at sites whose properties make them particularly attractive for exobiology. These critical experiments would include, for example, identification of organics, detection of fossils, and detection of extant life. The fourth phase is the detailed analysis of samples returned from these sites in Earth-based laboratories to confirm and extend previous discoveries. Finally, in the fifth phase, human exploration is needed to establish the geological settings for the earlier findings or to discover and explore sites that are not accessible to robotic spacecraft.     

Search for life on Mars: evaluation of techniques.

An important question for exobiology is, did life evolve on Mars? To answer this question, experiments must be conducted on the martian surface. Given current mission constraints on mass, power, and volume, these experiments can only be performed using proposed analytical techniques such as: electron microscopy, X-ray fluorescence, X-ray diffraction, alpha-proton backscatter, gamma-ray spectrometry, differential thermal analysis, differential scanning calorimetry, pyrolysis gas chromatography, mass spectrometry, and specific element detectors. Using prepared test samples consisting of 1% organic matter (bovine serum albumin) in palagonite and a mixture of palagonite, clays, iron oxides, and evaporites, it was determined that a combination of X-ray diffraction and differential thermal analysis coupled with gas chromatography provides the best insight into the chemistry, mineralogy, and geological history of the samples.     

Bio-markers and the search for extinct life on Mars.

Geologic and climatologic studies suggest that conditions on early Mars were similar to early Earth. Because life on Earth is believed to have originated during this early period (3.5 billion years ago), the Martian environment could have also been conducive to the origin of life. To investigate this possibility we must first define the attributes of an early Martian biota. Then, specific geographic locations on Mars must be chosen where life may have occurred (i.e. areas which had long standing water), and within these distinct locations search for key signatures or bio-markers of a possible extinct Martian biota. Some of the key signatures or bio-markers indicative of past biological activity on Earth may be applicable to Mars including: reduced carbon and nitrogen compounds, CO3(2-), SO4(2-), NO3-, NO2- [correction of NO2(2)], Mg, Mn, Fe, and certain other metals, and the isotopic ratios of C, N and S. However, we must also be able to distinguish abiotic from biologic origins for these bio-markers. For example, abiotically fixed N2 would form deposits of NO3- and NO2-, whereas biological processes would have reduced these to ammonium containing compounds, N2O, or N2, which would then be released to the atmosphere. A fully equipped Mars Rover might be able to perform analyses to measure most of these biomarkers while on the Martian surface.