Utilization of white potatoes in CELSS.

Potatoes (Solanum tuberosum) have a strong potential as a useful crop species in a functioning CELSS. The cultivar Denali has produced 37.5 g m-2 d-1 when grown for 132 days with the first 40 days under a 12-h photoperiod and a light:dark temperature cycle of 20 degrees C:16 degrees C, and then 92 days under continuous irradiance and a temperature of 16 degrees C. Irradiance was at 725 micromoles m-2 s-1 PPF and carbon dioxide at 1000 micromoles mol-1. The dried tubers had 82% carbohydrates, 9% protein and 0.6% fat. Other studies have shown that carbon dioxide supplementation (1000 micromoles mol-1) is of significant benefit under 12-h irradiance but less benefit under 24 h irradiance. Irradiance cycles of 60 minutes light and 30 minutes dark caused a reduction of more than 50% in tuber weight compared to cycles of 16 h light and 8 h dark. A diurnal temperature change of 22 degrees C for the 12-h light period to 14 degrees C during the 12-h dark period gave increased yields of 30% and 10% for two separate cultivars, compared with plants grown under a constant 18 degrees C temperature. Cultivar screening under continuous irradiance and elevated temperatures (28 degrees C) for 8 weeks of growth indicated that the cvs Haig, Denali, Atlantic, Desiree and Rutt had the best potential for tolerance to these conditions. Harvesting of tubers from plants at weekly intervals, beginning at 8 weeks after planting, did not increase yield over a single final harvest. Spacing of plants on 0.055 centers produced greater yield per m2 than spacing at 0.11 or 0.22 m2. Plants maintained 0.33 meters apart (0.111 m2 per plant) in beds produced the same yields when separated by dividers in the root matrix as when no separation was made.     

Particulated growth media for optimal liquid and gaseous fluxes to plant roots in microgravity.

An important and yet relatively under researched area of plant growth in microgravity, deals with the rooting environment of plants. A comprehensive approach for selecting the physical characteristics of root growth media which optimizes the dynamic availability of water and dissolved nutrients, and gases to plant roots was developed and tested. Physically-based and parametric models describing the relationship between content and fluxes of liquids and gases were used to cast a multi-objective optimization problem. This methodology reveals that a medium's ability to supply liquid and gas fluxes optimally is dependent upon physiological target values, system operation limits and root module design which dictate the medium's range of soil water characteristic and particle size distribution. Optimized media parameters designate a particle size distribution from which a particulated growth media was constructed and matched to the optimized media parameters. This methodology should improve the selection of optimal media properties for plant growth in microgravity as well as other porous media applications.     

点阵液晶显示器用于数字记忆示波器的显示方法

提出了一种点跟踪采样、逐帧实时显示的波形显示方法，成功的使点阵液晶显示器运用到数字记忆示波器的显示上，并以日本ＥＰＳＯＮ公司的Ｅ１３３０点阵液晶控制器为应用对象，介绍了具体显示方法。该方法具有较高的实用价值。     

Carbon dioxide interactions with irradiance and temperature in potatoes.

Separate controlled environment studies were conducted to determine the interaction of CO2 with irradiance and interaction of CO2 with temperature on growth of three potato cultivars. In the first study, an elevated CO2 concentration of 1000 micromoles mol-1 and an ambient CO2 of 350 micromoles mol-1 were maintained at the photosynthetic photon fluxes (PPF) of 17 and 34 mol m-2 d-1 with 12 h photoperiod, and at the PPF of 34 and 68 mol m-2 d-1 with 24 h photoperiod (400 and 800 micromoles m-2 s-1 PPF at each photoperiod). Tuber and total dry weights of 90-day old potatoes were significantly increased with CO2 enrichment, but the CO2 stimulation was less with higher PPF and longer photoperiod. Shoot dry weight was affected more by photoperiod than by PPF and CO2 concentrations. The elevated CO2 concentration increased leaf CO2 assimilation rates and decreased stomatal conductance with 12 h photoperiod, but had only a marginal effect with 24 h photoperiod. In the second study, four CO2 concentrations of 500, 1000, 1500 and 2000 micromoles mol-1 were combined with two air temperature regimes of 16 and 20 degrees C under a 12 h photoperiod. At harvest, 35 days after transplanting, tuber and total dry weights of potatoes reached a maximum with 1000 micromoles mol-1 CO2 at 16 degrees C, but continued to increase up to 2000 micromoles mol-1 CO2 at 20 degrees C. Plant growth was greater at 20 degrees C than at 16 degrees C under all CO2 concentrations. At 16 degrees C specific leaf weight increased substantially with increasing CO2 concentrations as compared to 500 micromoles mol-1 CO2, but increased only slightly at 20 degrees C. This suggests a carbohydrate build-up in the leaves at 16 degrees C temperature that reduces plant response to increased CO2 concentrations. The data in the two studies indicate that a PPF of 34 mol m-2 d-1, 20 degrees C temperature, and 1000-2000 micromoles mol-1 CO2 produces optimal tuber yield in potatoes.     

Ultrastructural and growth indices of Chlorella culture in multicomponent aquatic systems under space flight conditions.

Submicroscopic organization of Chlorella cells cultivated under space flight conditions in three-component aquatic system has been studied. Comparison of the experimental cells with that of the controls revealed certain rearrangements of cell organelles particularly, a reduction in the amount of reserve polysaccharides in chloroplasts, increase of cell vacuolization and mitochondrion volume, complication configuration of plasmalemma evaginations and invaginations, and also disturbances in the process of cytokinesis. More over an increase in the number of Chlorella cells infected by bacteria was shown in the experimental variant. No considerable differences were established in the growth characteristics of the experimental and control populations. A comparative cytological analysis revealed general regularities of organelles in Chlorella cells cultivated under space flight condition in the uni- and multicomponent systems.     

Trickle water and feeding system in plant culture and light-dark cycle effects on plant growth.

Rockwool, as an inert medium covered or bagged with polyethylene film, can be effectively used for plant culture in space station. The most important machine is the pump adjusting the dripping rate in the feeding system. Hydro-aeroponics may be adaptable to a space laboratory. The shortening of the light-dark cycles inhibits plant growth and induces an abnormal morphogenesis. A photoperiod of 12-hr-dark may be needed for plant growth.     

Instrumentation for plant health and growth.

Comprehensive spectroscopic monitoring of plant health and growth in bioregenerative life support system environments is possible using a variety of spectrometric technologies. Absorption spectrometry and atomic emission spectrometry in combination allow for direct, on-line, reagentless monitoring of plant nutrients from nitrate and potassium to micronutrients such as copper and zinc. Fluorometric spectrometry is ideal for the on-line detection, identification and quantification of bacteria and fungi. Liquid Atomic Emission Spectrometry (LAES) is a new form of spectrometry that allows for direct measurement of atomic emission spectra in liquids. An electric arc is generated by a pair of electrodes in the liquid to provide the energy necessary to break molecular bonds and reduce the substance to atomic form. With a fiber probe attached to the electrodes, spectral light can be transmitted to a photodiode array spectrometer for light dispersion and analysis. Ultraviolet (UV) absorption spectrometry is a long-established technology, but applications typically have required specific reagents to produce an analyte-specific absorption. Nitrate and iron nutrients have native UV absorption spectra that have been used to accurately determine nutrient concentrations at the +/- 5% level. Fluorescence detection and characterization of microbes is based upon the native fluorescent signatures of most microbiological species. Spectral and time-resolved fluorometers operating with remote fiber-optic probes will be used for on-line microbial monitoring in plant nutrient streams.     

Optical sensors for monitoring and control of plant growth systems.

Optical chemical sensors have been developed for monitoring several parameters relevant to plant growth systems. These sensors utilize porous polymer and porous glass as the sensing element, and optical fiber input/output lines connected to a custom optoelectronic interface. Present in the sensing element are immobilized colorimetric indicators, which react with the analyte to be sensed. This reaction results in a change in the optical properties of the sensor. These sensors are particularly suited to in-situ monitoring of nutrient solution parameters and atmospheric trace contaminants in life support and plant growth systems. Sensors for monitoring pH, ammonia, and ethylene will be discussed.     

Use of lunar regolith as a substrate for plant growth.

Regenerative Life Support Systems (RLSS) will be required to regenerate air, water, and wastes, and to produce food for human consumption during long-duration missions to the Moon and Mars. It may be possible to supplement some of the materials needed for a lunar RLSS from resources on the Moon. Natural materials at the lunar surface may be used for a variety of lunar RLSS needs, including (i) soils or solid-support substrates for plant growth, (ii) sources for extraction of essential, plant-growth nutrients, (iii) substrates for microbial populations in the degradation of wastes, (iv) sources of O2 and H2, which may be used to manufacture water, (v) feed stock materials for the synthesis of useful minerals (e.g., molecular sieves), and (vi) shielding materials surrounding the outpost structure to protect humans, plants, and microorganisms from harmful radiation. Use of indigenous lunar regolith as a terrestrial-like soil for plant growth could offer a solid support substrate, buffering capacity, nutrient source/storage/retention capabilities, and should be relatively easy to maintain. The lunar regolith could, with a suitable microbial population, play a role in waste renovation; much like terrestrial waste application directly on soils. Issues associated with potentially toxic elements, pH, nutrient availability, air and fluid movement parameters, and cation exchange capacity of lunar regolith need to be addressed before lunar materials can be used effectively as soils for plant growth.     

Neuronal feedback between brain and inner ear for growth of otoliths in fish.

Previous investigations revealed that fish inner ear otolith growth (concerning otolith size and calcium-incorporation) depends on the amplitude and the direction of gravity, suggesting the existence of a (negative) feedback mechanism. In search for the regulating unit, the vestibular nerve was unilaterally transected in neonate swordtail fish (Xiphophorus helleri) which were subsequently incubated in the calcium-tracer alizarin-complexone. Calcium incorporation ceased on the transected head sides, indicating that calcium uptake is neurally regulated.     

一种新型固体推进剂比冲标准物质

介绍一种新型的比冲标准物质BC-1,同SQ-2标准物质相比,对配方进行了改进,其燃烧性能和燃烧稳定性均优于SQ-2。通过对配方中燃烧催化系统的改进,研制出了一种新型的比冲标准物质BC-1,克服了SQ-2标准物质的燃烧不稳定性,且具有优良的燃烧特性,在6MPa~12 MPa压力范围内的压强指数由0.60降至-0.03。     

Validation of the in-flight calibration procedures for the MICROSCOPE space mission

The MICROSCOPE space mission aims to test the Equivalence Principle with an accuracy of 10^-15${10}^{-15}$. The drag-free micro-satellite will orbit around the Earth and embark a differential electrostatic accelerometer including two cylindrical test masses submitted to the same gravitational field and made of different materials. The experience consists in testing the equality of the electrostatic acceleration applied to the masses to maintain them relatively motionless. The accuracy of the measurements exploited for the test of the Equivalence Principle is limited by our a priori knowledge of several physical parameters of the instrument. These parameters are partially estimated on-ground, but with an insufficient accuracy, and an in-orbit calibration is therefore required to correct the measurements. The calibration procedures have been defined and their analytical performances have been evaluated. In addition, a simulator software including the dynamics model of the instrument, the satellite drag-free system and the perturbing environment has been developed to numerically validate the analytical results. After an overall presentation of the MICROSCOPE mission, this paper will describe the calibration procedures and focus on the simulator. Such an in-flight calibration is mandatory for similar space missions taking advantage of a drag-free system.     

A capillary-driven root module for plant growth in microgravity.

A new capillary-driven root module design for growing plants in microgravity was developed which requires minimal external control. Unlike existing systems, the water supply to the capillary-driven system is passive and relies on root uptake and media properties to develop driving gradients which operate a suction-induced flow control valve. A collapsible reservoir supplies water to the porous membrane which functions to maintain hydraulic continuity. Sheet and tubular membranes consisting of nylon, polyester and sintered porous stainless steel were tested. While finer pore sized membranes allow greater range of operation, they also reduce liquid flux thereby constraining system efficiency. Membrane selection should consider both the maximum anticipated liquid uptake rate and maximum operating matric head (suction) of the system. Matching growth media water retention characteristics to the porous membrane characteristics is essential for supplying adequate liquid flux and gas exchange. A minimum of 10% air-filled porosity (AFP) was necessary for adequate aeration. The capillary-driven module maintained hydraulic continuity and proper gas exchange rates for more than 80 days in a plant growth experiment.     

Convective motions in liquid metals for material processings. Numerical simulation of oscillation regimes and effect of rotation

The paper is concerned with the numerical simulation and the analysis of some kinds of flow regimes which can develop in Bridgman and Czochralski systems for material processings. The flows in the liquid phase are investigated considering two-dimensional and axisymmetric models. The time-dependent regimes were studied for a zero-Prandtl-number fluid layer confined inside a two-dimensional cavity of aspect ratio (length-to-height) A=4, involving a stress-free upper surface and submitted to a horizontal temperature gradient. The range of Grashof number was varied up to the conditions at which the flow goes from oscillatory to chaotic type behaviours. The combined influence of the temperature gradients and of the rotations of the crucible and of the seed/crystal was investigated for a Czochralski model. The axisymmetric regimes were studied for a Pr_m=0.015 liquid melt confined inside a cylindrical crucible of aspect ratio (height-to-radius) A_m=2, and coupled to a viscous encapsulant liquid layer (10<Pr_e<1200) of aspect ratio A_e=0.5. A number of steady and (transient) time-dependent flow patterns are identified.     

Development and growth of potato tubers in microgravity.

A potato explant consisting of a leaf, its axillary bud, and a small segment of stem will develop a tuber in 10-14 days when grown on earth. The tubers develop from the axillary buds and accumulate starch derived from sugars produced through photosynthesis and/or mobilized from leaf tissue. Potato explants were harvested and maintained in the Astroculture (TM) unit, a plant growth chamber designed for spaceflight. The unit provides an environment with controlled temperature, humidity, CO2 level, light intensity, and a nutrient delivery system. The hardware was loaded onto the space shuttle Columbia 24 hours prior to the launch of the STS-73 mission. Explant leaf tissue appeared turgid and green for the first 11 days of flight, but then became chlorotic and eventually necrotic by the end of the mission. The same events occurred to ground control explants with approximately the same timing. At the end of the 16-day mission, tubers were present on each explant. The size and shape of the space-grown tubers were similar to the ground-control tubers. The arrangement of cells in the tuber interior and at the exterior in the periderm was similar in both environments. Starch and protein were present in the tubers grown in space and on the ground. The range in starch grain size was similar in tubers from both environments, but the distribution of grains into size classes differed somewhat, with the space-grown tubers having more small grains than the ground control tubers. Proteinaceous crystals were found in tubers formed in each condition.     

Seedling growth and development on space shuttle.

Young pine seedlings, and mung bean and oat seeds were flown on shuttle flights, STS-3 and STS-51F, in March, 1982 and July/August, 1985, respectively. The plant growth units built to support the two experiments functioned mechanically as anticipated and provided the necessary support data. Pine seedlings exposed to the microgravity environment of the space shuttle for 8 days continued to grow at a rate similar to ground controls. Pine stems in flight seedlings, however, averaged 10 to 12% less lignin than controls. Flight mung beans grew slower than control beans and their stems contained about 25% less lignin than control seedlings. Reduced mung bean growth in microgravity was partly due to slower germination rate. Lignin also was reduced in flight oats as compared to controls. Oats and mung beans exhibited upward growing roots which were not observed in control seedlings. Chlorophyll A/B ratios were lower in flight tissues than controls. The sealed PGCs exhibited large variations in atmospheric gas composition but the changes were similar between flight and ground controls. Ethylene was present in low concentrations in all chambers.     

High temperature effect on microflora of radish root-inhabited zone and nutrient solutions for radish growth.

The effect of high temperatures (35 and 45 degrees C) on microflora of the root zone of radish plants grown in phytotron was evaluated by the response of microorganisms from 9 indicator groups. Phytotron air temperature elevated to 35 degrees C for 20 hours caused no significant changes in qualitative and quantitative composition of the root microflora in experimental plants. By the end of the experiment, the species diversity of microflora had changed. The amount of phytopathogenic microorganisms decreased which can be interpreted as more stable co-existence of microflora with plants. The numbers of microbes from other indicator groups was in dynamic equilibrium. The plants' condition did not deteriorate either. Exposure to the temperature of 45 degrees C for 7 hours have been found to change the numbers and species diversity in the radish root zone microflora. The microorganisms were observed to increase their total numbers at the expense of certain indicator groups. Bacteria increased spore forms at the stage of spores. Colon bacillus bacteria of increased their numbers by the end of experiment by an order. By the end of experiment the roots of experiment plants had microscopic fungi from Mucor, Aspergillus, Trichoderma, Cladosporium genera. The observed changes in the microbial complex seem to be associated with the changes of root emissions and general deterioration of the plants' condition. It is suggested that the response of the microorganisms can be indicative of the condition of plants under investigation.     

Achieving and documenting closure in plant growth facilities.

As NASA proceeds with its effort to develop a Controlled Ecological Life Support System (CELSS) that will provide life support to crews during long duration space missions, it must address the question of facility and system closure. Here we discuss the concept of closure as it pertains to CELSS and describe engineering specifications, construction problems and monitoring procedures used in the development and operation of a closed plant growth facility for the CELSS program. A plant growth facility is one of several modules required for a CELSS. A prototype of this module at Kennedy Space Center is the large (7m tall x 3.5m diameter) Biomass Production Chamber (BPC), the central facility of the CELSS Breadboard Project. The BPC is atmospherically sealed to a leak rate of approximately 5% of its total volume per 24 hours. This paper will discuss the requirements for atmospheric closure in this facility, present CO2 and trace gas data from initial tests of the BPC with and without plants, and describe how the chamber was sealed atmospherically. Implications that research conducted in this type of facility will have for the CELSS program are discussed.     

Development of tissue culture techniques and hardware to study mineralization under microgravity conditions.

To study the effects of weightlessness on mouse fetal long bone rudiment growth and mineralization we have developed a tissue culture system for the Biorack facility of Spacelab. The technique uses standard liquid tissue culture medium, supplemented with NA-beta-glycerophosphate, confined in gas permeable polyethylene bags mounted inside ESA Biorack Type I experiment containers. The containers can be flushed with an air/5% CO2 gas mixture necessary for the physiological bicarbonate buffer used. Small amounts of fluid can be introduced at the beginning (e.g. radioactive labels for incorporation studies) or at the end of the experiment (fixatives). A certain form of mechanical stimulation (continuous compression) can be used to counteract the, possibly, adverse effect of microgravity. Using 16 day old metatarsals the in vitro calcification process under microgravity conditions can be studied for a 4 day period.     

Gravi- and photostimuli in moss protonema growth movements.

Moss protonemal growth direction is controlled by at least three factors, photo-, gravi- and autotropism. It is possible to experimentally separate these factors and to control selectively their morphological appearance. In darkness protonema grow negatively gravitropically, and unilateral illumination initiated positive phototropism. Red light suppressed auto- and gravitropism, blue light suppressed only gravitropism. Green light allowed both gravi- and autotropism. The effect of light on gravitropism might involve changes in starch synthesis.