Effect of physical fitness and training on physiological responses to hypogravity.

The studies on the orthostatic tolerance during the hypodynamics exposure seem to be significant in connection with the selection, training and health maintenance of astronauts. Using male human subjects of various physical fitness levels, fluctuations of their physical fitness through 2 weeks of vigorous athletic training were measured in many parameters. For some of the subjects, the effects of 6 hr thermal neutral water immersion exposure in head out supine position on the physical fitness parameters and orthostatic tolerability were compared before training with after training. The results obtained were as follows: (1) Before training, orthostatic tolerability before hypodynamics exposure increased, following the physical fitness levels; the value after the hypodynamics exposure decreased in all the cases, but no differences were observed between the physical fitness levels. (2) As a result of training an increase of the physical fitness capacity was observed. The increase of orthostatic tolerability before hypodynamics exposure was noticed except for athletes. (3) Before hypodynamics exposure the urinary excretion of noradrenaline on non-athlete subjects increased as the physicsl fitness level increased. The values were decreased by physical training, the more so the better the physical fitness. After hypodynamics exposure the same relation was observed. But for athletes the values remain more stable and the decrease by hypodynamics exposure was not so distinctive. Such decreased reaction to hypodynamic conditions seems to reveal the neuro hormonal mechanism for the detrimental adaptation of athletes to hypodynamics. These results suggest that stable athletes do not always have low orthostatic tolerability, but do not respond well to hypodynamic conditions, at least from the orthostatic point of view. The mechanism seems related to sympathetic nerve activity.     

Mechanisms of spinal motoneurons survival in rats under simulated hypogravity on earth

It was previously shown that different cell types in vivo and in vitro may die via apoptosis under weightlessness conditions in space as well as in simulated hypogravity on the Earth. We assessed survivability of spinal motoneurons of rats after 35-day antiorthostatic hind limb suspension. Following weight bearing, unloading the total protein content in lumbar spinal cord is dropped by 21%. The electrophysiological studies of m. gastrocnemius revealed an elevated motoneurons’ reflex excitability and conduction disturbances in the sciatic nerve axons. The number of myelinated fibers in the ventral root of experimental animals was insignificantly increased by 35-day of antiorthostatic hind limb suspension, although the retrograde axonal transport was significantly decreased during the first week of simulated hypogravity. The results of the immunohistochemical assay with antibodies against proapoptotic protein caspase 9 and cytotoxicity marker neuron specific nitric oxide synthase (nNOS) and the TUNEL staining did not reveal any signs of apoptosis in motoneurons of suspended and control animals. To examine the possible adaptation mechanisms activated in motoneurons in response to simulated hypogravity we investigated immunoexpression of Hsp25 and Hsp70 in lumbar spinal cord of the rats after 35-day antiorthostatic hind limb suspension. Comparative analysis of the immunohistochemical reaction with anti-Hsp25 antibodies revealed differential staining of motoneurons in intact and experimental animals. The density of immunoprecipitate with anti-Hsp25 antibodies was substantially higher in motoneurons of the 35-day suspended than control rats and the more intensive precipitate in this reaction was observed in motoneuron neuritis. Quantitative analysis of Hsp25 expression demonstrated an increase in the Hsp25 level by 95% in experimental rats compared to the control. The immunoexpression of Hsp70 found no qualitative and quantitative differences in control and experimental lumbar spinal cords. Taken together our results show that (1) rat motoneurons survived after 35-day antiorthostatic hind limb suspension and the changes in neurons had a mostly functional character, and (2) the increased immunoexpression of Hsp25 can be considered as the anti-apoptotic factor.     

Response and adaptation of bone cells to simulated microgravity

Bone loss induced by microgravity during space flight is one of the most deleterious factors on astronaut’s health and is mainly attributed to an unbalance in the process of bone remodeling. Studies from the space microgravity have demonstrated that the disruption of bone remodeling is associated with the changes of four main functional bone cells, including osteoblast, osteoclast, osteocyte, and mesenchymal stem cells. For the limited availability, expensive costs and confined experiment conditions for conducting space microgravity studies, the mechanism of bone cells response and adaptation to microgravity is still unclear. Therefore, some ground-based simulated microgravity methods have been developed to investigate the bioeffects of microgravity and the mechanisms. Here, based on our studies and others, we review how bone cells (osteoblasts, osteoclasts, osteocytes and mesenchymal stem cells) respond and adapt to simulated microgravity.     

Induction of vascular endothelial phenotype and cellular proliferation from human cord blood stem cells cultured in simulated microgravity

Recent studies have demonstrated that stem cells derived from adult hematopoietic tissues are capable of trans-differentiation into non-hematopoietic cells, and that the culture in microgravity (microg) may modulate the proliferation and differentiation. We investigated the application of microg to human umbilical cord blood stem cells (CBSC) in the induction of vascular endothelial phenotype expression and cellular proliferation. CD34+ mononuclear cells were isolated from waste human umbilical cord blood samples and cultured in simulated microg for 14 days. The cells were seeded in rotary wall vessels (RWV) with or without microcarrier beads (MCB) and vascular endothelial growth factor was added during culture. Controls consisted of culture in 1 G. The cell cultures in RWV were examined by inverted microscopy. Cell counts, endothelial cell and leukocyte markers performed by flow cytometry and FACS scan were assayed at days 1, 4, 7 and at the termination of the experiments. Culture in RWV revealed significantly increased cellular proliferation with three-dimensional (3D) tissue-like aggregates. At day 4, CD34+ cells cultured in RWV bioreactor without MCB developed vascular tubular assemblies and exhibited endothelial phenotypic markers. These data suggest that CD34+ human umbilical cord blood progenitors are capable of trans-differentiation into vascular endothelial cell phenotype and assemble into 3D tissue structures. Culture of CBSC in simulated microg may be potentially beneficial in the fields of stem cell biology and somatic cell therapy.     

从对《安妮日记》的改写看奈达的读者反应论

文章基于"Translation:Ideology——On the Construction of Different Anne Franks"一文中关于《安妮日记》不同版本改写,删减的问题,举例说明了读者反应论在跨文化翻译中的作用。     

Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.

The cytoskeleton is a complex network of fibers that is sensitive to environmental factors including microgravity and altered gravitational forces. Cellular functions such as transport of cell organelles depend on cytoskeletal integrity; regulation of cytoskeletal activity plays a role in cell maintenance, cell division, and apoptosis. Here we report cytoskeletal and mitochondria alterations in cultured human lymphocyte (Jurkat) cells after exposure to spaceflight and in insect cells of Drosophila melanogaster (Schneider S-1) after exposure to conditions created by clinostat rotation. Jurkat cells were flown on the space shuttle in Biorack cassettes while Schneider S-1 cells were exposed to altered gravity forces as produced by clinostat rotation. The effects of both treatments were similar in the different cell types. Fifty percent of cells displayed effects on the microtubule network in both cell lines. Under these experimental conditions mitochondria clustering and morphological alterations of mitochondrial cristae was observed to various degrees after 4 and 48 hours of culture. Jurkat cells underwent cell divisions during exposure to spaceflight but a large number of apoptotic cells was also observed. Similar results were obtained in Schneider S-1 cells cultured under clinostat rotation. Both cell lines displayed mitochondria abnormalities and mitochondria clustering toward one side of the cells which is interpreted to be the result of microtubule disruption and failure of mitochondria transport along microtubules. The number of mitochondria was increased in cells exposed to altered gravity while cristae morphology was severely affected indicating altered mitochondria function. These results show that spaceflight as well as altered gravity produced by clinostat rotation affects microtubule and mitochondria organization and results in increases in apoptosis. Grant numbers: NAG 10-0224, NAG2-985.     

高温环境中碳化型烧蚀材料的详细响应

阐述并分析了高温环境下烧蚀材料的详细响应.给出了固定坐标系下控制偏微分方程及其隐式有限差分的形式.在反应区,详细的高温分解动力学服从阿仑尼乌斯定律,热物理性质与温度和状态有关.给出了评价材料热性质的数学函数,采用适当方法使计算时间大幅降低.设计了一套计算机软件,用该软件计算得出的结果与标准软件结果、公开的文献报道和内部的试验结果相一致.     

A preliminary survey of non-lichenized fungi cultured from the hyperarid Atacama Desert of Chile

The Atacama Desert is one of the driest environments on Earth, and has been so for over 200,000 years. Previous reports have suggested that surprisingly low numbers of culturable bacteria, counted as biomass or species diversity, are present in Atacama sands collected from the most hyperarid regions. In previous studies, the presence of eukaryotic organisms was not discussed. In this report, we describe a method of direct plating onto rich media that resulted in culturing a range of fungi from Atacama samples. All fungi identified in this preliminary survey are spore-forming saprobes that are readily dispersed by wind, a likely mechanism that accounts for their presence in the central Atacama Desert.     

Taxane recovery from cells of Taxus in micro- and hypergravity

Cell suspension cultures of Taxus cuspidata produce taxanes that are released from the outer surface of cells into the culture medium as free and bound alkaloids. Paclitaxel (Taxol (TM)), is an anti-cancer drug in short supply. It has a taxane ring derived from baccatin III and a C-13 phenylisoserine side-chain. This drug is produced over a wide range of gravitational forces. Monoclonal and polyclonal antibodies to paclitaxel, baccatin III, and the C-13 phenylisoserine side chain were combined in multiple-labeling studies to localize taxanes and paclitaxel on cell surfaces or on particles released into the culture medium. Bioreactor vessel design altered the composition of taxanes recovered from cells in simulated microgravity. At 10(-2) and 2x10(-4)g, taxane recovery was reduced but biomass growth and percent paclitaxel was significantly increased. At 1 to 24g, growth was reduced with a significant recovery of total taxanes with low percent paclitaxel. Bound paclitaxel was also localized in endonuclease-rich fragmenting nuclei of individual apoptotic cells. A model is presented comprising TCH (touch) genes encoding enzymes that modify taxane-bearing xylan residues in cell walls, the calcium-sensing of gravitational forces by the cytoplasm, and the predisposition of nuclei to apoptosis. This integrates the adaptive physiological and biochemical responses of drug-producing genomes with gravitational forces.     

车载设备的振动响应

本文用力阻抗法推导出车载设备在受车内动力设备或地面振动干扰时的振动响应公式。     

Simultaneous Response of the Mid-Latitude Ionosphere to a Sudden Commencement of Strong Magnetic Storms

Using the new technology of global GPS detection of ionospheric disturbances (GLOBDET), it is found that a sharp increase of the time derivative of the magnetic field strength during magnetic storms is accompanied by a simultaneous decrease of the mid-latitude total electron content (TEC) over the entire dayside of the globe. The corresponding negative correlation coefficient is no less than 0.8, and the delay relative to the sudden commencement of magnetic storm is about 3–10 min. The effect is especially clearly pronounced for magnetic storms with sudden commencements (SSC). The analysis is carried out for a set of 90 to 300 GPS stations for 10 days (January 6 and April 23, 1998; April 6, June 8, July 13, 14, and 15, 2000; March 31, April 4 and 11, 2001) with various levels of geomagnetic activity (D _st and K _p varied from –6 to –295 nT and from 0 to 9, respectively). The amplitude of the response in the total electron content for the events considered was 0.1–0.4 × 10¹⁶ m^–2 (which is a deviation of 0.2–2.6% from the TEC background value). The velocity of the disturbance motion from the dayside to the nightside was about 10–20 km/s. The results obtained agree with the data of ionospheric parameter measurements conducted earlier by methods with high temporal resolution.     

某天线支承筒阻尼减振设计与分析

为减小某卫星天线支承筒的振动,根据阻尼减振原理,采用约束阻尼层方法进行减振处理。以ZN-1丁基橡胶为粘性阻尼材料,T700S层合板为约束层,在约束层厚0.4 mm,铺层数为4的条件下,设计了三种不同铺层角的减振方案。有限元计算结果表明,在天线支承筒上附加约束阻尼层可明显降低支承筒指定点处的频率响应。在给定条件下,给出的三种方案均满足工程要求,其中以铺层角45°/-45°/0°/90°方案的效果最佳。     

砂垫层刚度对地面地震反应的影响研究

我国是一个多地震国家,地震区分布范围广,发震频繁.因此减震、隔震技术对国民经济基本建设具有重要意义.随着科学技术的进步、计算机的运用等,减震、隔震方法的研究与应用取得了很大的发展,但仍有许多问题有待解决,特别是就我国目前中、低层房屋较多的实际状况而言,这一问题显得尤为突出.采用在基础与地基之间设置砂垫层的方法,减小或不放大地震对地面的影响,最终达到减震、隔震的目的.在提出砂垫层隔震概念及其与<建筑抗震设计规范>(GB50011-2001)的区别之后,研究了砂垫层刚度对地面地震反应的影响,得到了一些有益的结果.     

Microbial fuel cells applied to the metabolically based detection of extraterrestrial life

Since the 1970s, when the Viking spacecrafts carried out experiments to detect microbial metabolism on the surface of Mars, the search for nonspecific methods to detect life in situ has been one of the goals of astrobiology. It is usually required that a methodology detect life independently from its composition or form and that the chosen biological signature point to a feature common to all living systems, such as the presence of metabolism. In this paper, we evaluate the use of microbial fuel cells (MFCs) for the detection of microbial life in situ. MFCs are electrochemical devices originally developed as power electrical sources and can be described as fuel cells in which the anode is submerged in a medium that contains microorganisms. These microorganisms, as part of their metabolic process, oxidize organic material, releasing electrons that contribute to the electric current, which is therefore proportional to metabolic and other redox processes. We show that power and current density values measured in MFCs that use microorganism cultures or soil samples in the anode are much larger than those obtained with a medium free of microorganisms or sterilized soil samples, respectively. In particular, we found that this is true for extremophiles, which have been proposed as potential inhabitants of extraterrestrial environments. Therefore, our results show that MFCs have the potential to be used for in situ detection of microbial life.     

砂垫层厚度及基底压力对地面地震反应的影响

我国地震区分布范围广，发震频繁。随着科学技术的进步、计算机的运用等，国内外对建筑结构减震、隔震方法的研究与应用取得了很大的发展，但仍有许多问题有待解决。特别是我国为农业大国，目前中、低层房屋仍然较多，采用在基础与地基之间设置砂垫层的方法，以最少的造价，达到减小或不放大地震对地面的影响，最终达到减震、隔震的目的是一种切合实际的方法。在研究了砂垫层厚度及基底压力对地面地震反应的影响后，得到了一些有益的结果。     

债转股问题初探

债转股作为经济体制改革的一个重要探索,已经成为当今法学界和经济学界讨论的主题.作为一个新事物的出现,债转股必然会遇到一系列来自不同方向的阻碍,本文就这些困惑进行论述,阐明了债转股面临的主要问题,并提出相应的解决方案和建议.国民经济体制改革需要大胆的探索和创新,债转股就是一个很好的范例,尽管其实施过程中难免遇到一些难题,但必将取得骄人的成果.     

EDFA对HFC系统CNR的影响

在光纤接入网中EDFA是不可缺少的部件,在HFC网络中,EDFA主要作用是将光功率放大使光信号传输距离更远或光分路能力更强。论文从分析HFC系统的CNR入手,对HFC系统中使用的。EDFA的CNR进行了较详细理论的分析,阐述了EDFA对HFC系统CNR的影响。     

案例教学法在高职高专营销专业教学中存在问题的研究

案例教学是一种全新的教学模式。尽管首创于美国,但对我国高校类似专业的教学模式仍有借鉴意义,需要任课教师与学生的互动才能实现其教学效果。文章从四个方面论述了影响案例教学应用于营销类专业教学环节中存在的一些问题,并且提出了一些试图解决这些问题的思路和方法。具有一定的现实意义。     

Transfer orbits to/from the Lagrangian points in the restricted four-body problem

The well-known Lagrangian points that appear in the planar restricted three-body problem are very important for astronautical applications. They are five points of equilibrium in the equations of motion, what means that a particle located at one of those points with zero velocity will remain there indefinitely. The collinear points (L₁, L₂ and L₃) are always unstable and the triangular points (L₄ and L₅) are stable in the present case studied (Earth–Sun system). They are all very good points to locate a space-station, since they require a small amount of ΔV (and fuel), the control to be used, for station-keeping. The triangular points are especially good for this purpose, since they are stable equilibrium points.In this paper, the planar restricted four-body problem applied to the Sun–Earth–Moon–Spacecraft is combined with numerical integration and gradient methods to solve the two-point boundary value problem. This combination is applied to the search of families of transfer orbits between the Lagrangian points and the Earth, in the Earth–Sun system, with the minimum possible cost of the control used. So, the final goal of this paper is to find the magnitude of the two impulses to be applied in the spacecraft to complete the transfer: the first one when leaving/arriving at the Lagrangian point and the second one when arriving/living at the Earth.The dynamics given by the restricted four-body problem is used to obtain the trajectory of the spacecraft, but not the position of the equilibrium points. Their position is taken from the restricted three-body model. The goal to use this model is to evaluate the perturbation of the Sun in those important trajectories, in terms of fuel consumption and time of flight. The solutions will also show how to apply the impulses to accomplish the transfers under this force model.The results showed a large collection of transfers, and that there are initial conditions (position of the Sun with respect to the other bodies) where the force of the Sun can be used to reduce the cost of the transfers.