Metabolic changes in rats subjected to space flight for 18.5 days in the biosatellite Cosmos 936

From an investigation of the activity of six glucocorticoid dependent liver enzymes, the existence of chronic, transient, stress-induced hypercorticosteronaemia during flight is probable. This hypercorticosteronaemia arises from weightlessness and induces gluconeogenesis. Weightlessness also caused substantial increases in liver glycogen level. The increased lipolytic activity and that of lipoprotein lipase in several groups of animals could be interpreted as enhancement of fat mobilization and utilization under the influence of stress. As this latter enhancement was also found in ground-based controls, it may have been due to the stress of handling rather than to space flight per se.     

Changes of deoxyribonucleoprotein in the spleen, thymus and liver of rats exposed to weightlessness and artificial gravity aboard the Cosmos biosatellites

Changes of deoxyribonucleoprotein in the spleen, thymus and liver of rats exposed to wegithlessness or artifical gravity on board biosatellites Cosmos 782 and Cosmos 936 after 20 days of flight were investigated. The level of polydeoxyribonucleotides in the spleen and thymus of rats exposed during the flight to weightlessness increased 4 – 11 hours after landing, suggesting breakdown of a part of the deoxyribonucleoprotein present. The use of artifical gravity prevented this breakdown in the thymus but not in the spleen. The breakdown was accompanied in the majority of cases by a decrease in teh deoxyribonucleoprotein content. We believe the breakdown of deoxyribonucleoprotein is due to a nonspecific stress reaction to the change from the weightless state to that of terrestrial gravity during landing. The polydeoxyribonucleotide level and amount of deoxyribonucleoprotein in the majority of cases returned to normal values during the 25 days of readaptation. No substantial change of deoxyribonucleoprotein was found in the liver. The different findings in the three organs are due to the fact that breakdown of deoxyribonucleoprotein takes place in sensitive cells underlying pycnosis. These cells are found in the spleen and thymus, but not in the liver.     

Biological role of gravity: hypotheses and results of experiments on "Cosmos" biosatellites.

In order to reveal the biological significance of gravity, microgravity effects have been studied at the cellular, organism and population levels. The following questions arise. Do any gravity-dependent processes exist in a cell? Is cell adaptation to weightlessness possible; if so, what role may cytoskeleton, the genetic apparatus play in it? What are the consequences of the lack of convection in weightlessness for the performance of morphogenesis? Do the integral characteristics of living beings change in weightlessness? Is there any change in "biological capacity" of space, its resistance to expansion of life? What are the direction and intensity of microgravity action as a factor of natural selection, the driving force of evolution? These problems are discussed from a theoretical point of view, and in the light of results obtained in experiments from aboard biosatellites "Cosmos".     

The effect of gravity on plant germination.

An axis clinostat was constructed to create micro and negative gravity also a rotated flat disk was constructed with different rotation rates to give increased gravity, by centrifugal force up to 48 g. Rice seeds were grown on agar in tubes at the constant air temperature of 20 degrees C under an average light condition of 110 micromol/m2/sec(PPF). Humidity was not controlled but was maintained above 90%. Since the tube containers were not large enough for long cultivation, shoot and root growth were observed every 12 hours until the sixth day from seeding. The lengths of shoots and roots for each individual plant were measured on the last day. The stem lengths were increased by microgravity but the root lengths were not. Under the negative gravity, negative orthogeotropism and under microgravity, diageotropism was observed. No significant effect of increased gravity was observed on shoot and root growth.     

Operation of magnetically assisted fluidized beds in microgravity and variable gravity: experiment and theory.

The conversion of solid waste into useful resources in support of long duration manned missions in space presents serious technological challenges. Several technologies, including supercritical water oxidation, microwave powered combustion and fluidized bed incineration, have been tested for the conversion of solid waste. However, none of these technologies are compatible with microgravity or hypogravity operating conditions. In this paper, we present the gradient magnetically assisted fluidized bed (G-MAFB) as a promising operating platform for fluidized bed operations in the space environment. Our experimental and theoretical work has resulted in both the development of a theoretical model based on fundamental principles for the design of the G-MAFB, and also the practical implementation of the G-MAFB in the filtration and destruction of solid biomass waste particles from liquid streams.     

The effect of temperature gradient on the motion of a bubble in reduced gravity

The bubble motion is described as a function of thermal variation of surface tension of a bubble, temperature gradient, gravity, bubble diameter, viscosity and density. The relations among those values are shown in graphs, from which the requirements for the measurements to ascertain the velocity due to thermal variation of surface tension are made clear. According to these requirements, the experiments on the velocity of the bubble in a temperature gradient were conducted and the Marangoni effect on a bubble motion was ascertained.     

Results on Artemia cysts, lettuce and tobacco seeds in the Biobloc 4 experiment flown aboard the Soviet Biosatellite Cosmos 1129.

Artemia cysts, lettuce and tobacco seeds were flown aboard the Cosmos 1129 for 19 days. A correlative method was used in order to determine the passage of cosmic heavy ions (HZE particles) through the biological test objects. This space flight resulted in a decrease on hatchability, nucleic acid and protein synthesis in hydrated Artemia cysts. HZE particle effects on plant cellular chromosomes are confirmed. In tobacco seeds, a stimulating effect on germination rate and a higher frequency of abnormalities were observed. Dormant biological objects are a very suitable material to study cosmic ray effects: these objects can be arranged in monolayers and sandwiched between visual track detectors in order to determine the passage of the cosmic heavy ions (HZE particles). On the other hand this method allows us to study effects of microgravity and those of the protonic component of cosmic rays in the objects not hit by the HZE articles.     

The effect of spatial truncation error on integral inversion of satellite gravity gradiometry data

The satellite gravity gradiometry (SGG) data can be used for local modelling of the Earth’s gravity field. In this study, the SGG data in the local north-oriented and orbital frames are inverted to the gravity anomaly at sea level using the second-order partial derivatives of the extended Stokes formula. The emphasis is on the spatial truncation error and the kernel behaviour of the integral formulas in the aforementioned frames. The paper will show that only the diagonal elements of gravitational tensor at satellite level are suitable for recovering the gravity anomaly at sea level. Numerical studies show that the gravity anomaly can be recovered in Fennoscandia with an accuracy of about 6 mGal directly from on-orbit SGG data.     

Formation and growth of callus tissue of Arabidopsis under changed gravity.

The changed gravity conditions do not prevent the process of cell dedifferentiation and formation of callus. Yet, callus grown on the clinostat and in space differs from the control one: its consistence is denser, occurence of meristematic centres is less frequent and it shows a reduced proliferative activity of cells. Average size of cell and nuclei area in the test variants is lower than in the control.     

The ionospheric effect of atmospheric gravity waves excited prior to strong earthquake

We have computed perturbations in the nighttime mid-latitude F2 region ionosphere that could be produced by internal atmospheric gravity waves generated before strong earthquakes through ionospheric Joule heating due to the seismogenic electric field of short duration. There is a strong anisotropy of the atmospheric gravity wave effect with respect to the imminent earthquake epicentre, the electron density changes being maximum poleward and equatorward of the epicentre and being minimum eastward and westward of it. It should be noted that the duration of the electron density perturbation in the F2 region ionosphere is much longer than the duration of the primary precursor of an earthquake – the enhancement of the vertical electric field at the Earth’s surface, which initiates the atmospheric gravity wave generation. This fact is important from the practical point of view of predicting catastrophic earthquakes.     

Results of ZARNITZA-2, a rocket experiment on artificial electron beam injection in the ionosphere

The rocket experiment ZARNITZA-2 on artificial electron beam injection in the ionosphere was carried out on September 11, 1975. Great attention was paid to the study of phenomena occurring in the near-rocket region. The results of the experiment are discussed in terms of a beam plasma discharge.     

Registration of ELF waves in rocket-satellite experiment with plasma injection

Two rocket experiments KOMBI-SAMA with plasma injection at height 100–240 km were performed in August 1987 in the region of Brazilian magnetic anomaly (L = 1.25). The launching time of the rocket was determined so that plasma injection was at the time when satellite COSMOS 1809 passed as close as possible to magnetic tube of injection. Caesium plasma jet was produced during ≥ 300 s by electric plasma generator separated from the payload. By diagnostic instruments on board of the rocket and the satellite were registered energetic particle fluxes and plasma wave activities stimulated by plasma injection. When the satellite passed the geomagnetic tube intersecting the injection region an enhancement of ELF emission at 140 Hz, 450 Hz by 2 times was registered on board the satellite. An enhancement of energetic particles (E > 40 keV) flux by 4–5 times was registered on board the rocket. Observed ELV emission below 100 Hz is interpreted as generation of oblique electromagnetic ion-cyclotron waves due to drift plasma instability at the front of the plasma jet.     

The influence of gravity on structure and function of animals

Gravity is the only environmental parameter that has remained constant during the period of evolution of living matter on Earth. Thus, it must have been a major force in shaping livimg things. The influence of gravitational loading on evolution of the vertebrate skeleton is well recognized, and scale effects have been studied. This paper, however, considers in addition four pivotal events in early evolution that would seem to have been significant for the later success and diversification of animal life. These are evolution of the cytoskeleton, cell motility (flagellae and cilia), gravity detecting devices (accelerometers), and biomineralization. All are functionally calcium dependent in eukaryotes and all occurred or were foreshadowed in prokaryotes. A major question is why calcium was selected as an ion of great importance to the structure and function of living matter; another is whether gravity played a role in its selection.     

A drop-tower experiment to determine the threshold of gravity for inducing motion sickness in fish.

It has been repeatedly shown earlier that some fish of a given batch reveal motion sickness (a kinetosis) at the transition from 1 g to microgravity. In the course of parabolic aircraft flight experiments, it has been demonstrated that kinetosis susceptibility is correlated with asymmetric inner ear otoliths (i.e., differently weighed statoliths on the right and the left side of the head) or with genetically predispositioned malformed cells within the sensory epithelia of the inner ear. Hitherto, the threshold of gravity perception for inducing kinetotic behavior as well as the relative importance of asymmetric otoliths versus malformed epithelia for kinetosis susceptibility has yet not been determined. The following experiment using the ZARM drop-tower facility in Bremen, Germany, is proposed to be carried out in order to answer the aforementioned questions. Larval cichlid fish (Oreochromis mossambicus) will be kept in a camcorder-equipped centrifuge during the microgravity phases of the drops and thus receive various gravity environments ranging from 0.1 to 0.9 g. Videographed controls will be housed outside of the centrifuge receiving 0 g. Based on the video-recordings, animals will be grouped into kinetotically and normally swimming samples. Subsequently, otoliths will be dissected and their size and asymmetry will be measured. Further investigations will focus on the numerical quantification of inner ear supporting and sensory cells as well as on the quantification of inner ear carbonic anhydrase reactivity. A correlation between: (1) the results to be obtained concerning the g-loads inducing kinetosis and (2) the corresponding otolith asymmetry/morphology of sensory epithelia/carbonic anhydrase reactivity will further contribute to the understanding of the origin of kinetosis susceptibility. Besides an outline of the proposed principal experiments, the present study reports on a first series of drop-tower tests, which were undertaken to elucidate the feasibility of the proposal (especially concerning the question, if some 4.7 s of microgravity are sufficient to induce kinetotic behavior in larval fish).     

Influence of altered gravity on brain cellular energy and plasma membrane metabolism of developing lower aquatic vertebrates.

Biochemical analyses of the brain of cichlid fish larvae, exposed for 7 days to increased acceleration of 3g (hyper-g), revealed an increase in energy availability (succinate dehydrogenase activity, SDH), and in mitochondrial energy transformation (creatine kinase, Mia-CK), but no changes in an energy consumptive process (high-affinity Ca(2+)-ATPase). Brain glucose-6-phosphate dehydrogenase (G6PDH) of developing fish was previously found to be increased after hyper-g exposure. Three respectively 5 hours thereafter dramatic fluctuations in enzyme activity were registered. Analysing the cytosolic or plasma membrane-located brain creatine kinase (BB-CK) of clawed toad larvae after long-term hyper-g exposure a significant increase in enzyme activity was demonstrated, whereas the activity of a high affinity Ca(2+)-ATPase remained unaffected.     

A direct approach to the study of the effect of gravity on axis formation in birds.

A system has been developed to enable the normal development of aborted very early uterine avian embryos, outside the female's uterus. The shell-less aborted egg was put into a foster shell of a sister egg, previously laid by the same female. The empty space between the shell and aborted egg was filled with artificial uterine fluid. The reconstructed eggs were incubated at 42 degrees C for 30 hours in a vertical position. The atmosphere contained a high concentration of CO2 (8-10%). At the termination of the 30 h the eggs were transferred to incubation at 37 degrees C in normal atmospheric conditions. Normal development has been recorded for a certain percentage of eggs incubated up to 12 days. In other cases abnormalities, arrested development or development of extraembryonic membranes only, without a sign of an embryonic axis, have been observed. The three important conclusions from the above experiments were: 1. It is possible to develop a closed, self-contained system, disconnected from the female's body, that would support the development of early uterine embryos. 2. The incidence of embryo-less extraembryonic membranes in such a system, is correlated with the degree of detachment of the "yolk" from the outer envelopes. 3. Such a system can be further developed into an experiment suited for microgravity conditions which will be an alternative to an experiment with live birds. The experiment will be aimed at testing the importance of gravity in changing the radially symmetrical avian blastoderm into a bilaterally symmetrical blastoderm.     

The ORGANIC experiment on EXPOSE-R on the ISS: Flight sample preparation and ground control spectroscopy

In March of 2009, the ORGANIC experiment integrated into the European multi-user facility EXPOSE-R, containing experiments dedicated to Astrobiology, was mounted through Extra Vehicular Activity (EVA) externally on the International Space Station (ISS). The experiment exposed organic samples of astronomical interest for a duration of 97 weeks (∼22 months) to the space environment. The samples that were returned to Earth in spring 2011, received a total UV radiation dose during their exposure including direct solar irradiation of >2500 h, exceeding the limits of laboratory simulations. We report flight sample preparation and pre-flight ultraviolet–visible (UV–Vis) characterization of the ORGANIC samples, which include 11 polycyclic aromatic hydrocarbons (PAHs) and three fullerenes. The corresponding time-dependent ground control monitoring experiments for ORGANIC measured over ∼19 months are presented and the results anticipated upon return of the samples are discussed. We present the first UV–Vis spectrum of solid circobiphenyl (C₃₈H₁₆). Further, we present the first published UV–Vis spectra of diphenanthro[9,10-b′,10′-d]thiophene (C₂₈H₁₆S), dinaphtho[8,1,2-abc,2′,1′,8′-klm]coronene (C₃₆H₁₆), tetrabenzo[de,no,st,c′d′]heptacene (C₄₂H₂₂), and dibenzo[jk,a′b′]octacene (C₄₀H₂₂) in solid phase and in solution. The results of the ORGANIC experiment are expected to enhance our knowledge of the evolution and degradation of large carbon-containing molecules in space environments.     

Modeling of artificial plasma “bubble” in ionosphere

Results of a combination of radio-crossing and in situ measurements of plasma density in an artificial plasma “bubble” in the ionosphere are presented. Shaped — charge barium injection was made at short distance (≤50 m) to plasma diagnostics on the rocket. After injection the rocket passed through expanding plasma shell. Plasma density depletion inside was more than one order and plasma enhancements on the boundary about 3–5 times that of background. When the rocket passed the shell and went away by 2.1 km an abrupt drop of telemetry signal level (≤ 65 dB) was registered though plasma density was not more than 3×10³sm⁻³. An estimation of high frequency signal refraction on the plasma shell is in good accordance with refraction data of geostationary satellite signals on equatorial bubbles.     

The influence of gravity on the process of development of animal systems

The development of animal systems is described in terms of a series of overlapping phases: pattern specification; differentiation; growth; and aging. The extent to which altered (micro) gravity (g) affects those phases is briefly reviewed for several animal systems. As a model, amphibian egg/early embryo is described. Recent data derived from clinostat protocols indicates that microgravity simulation alters early pattern specification (dorsal/ventral polarity) but does not adversely influence subsequent morphogenesis. Possible explanations for the absence of catastrophic microgravity effects on amphibian embryogenesis are discussed.