Motor coordination in weightless conditions revealed by long-term microgravity adaptation.

The functional approach to studying human motor systems attempts to give a better understanding of the processes behind planning movements and their coordinated performance by relying on weightlessness as a particularly enlightening experimental condition. Indeed, quantitative monitoring of sensorimotor adaptation of subjects exposed to weightlessness outlines the functional role of gravity in motor and postural organization. The recent accessibility of the MIR Space Station has allowed for the first time experimental quantitative kinematic analysis of long-term sensorimotor and postural adaptation to the weightless environment though opto-electronic techniques. In the frame of the EUROMIR'95 Mission, two protocols of voluntary posture perturbation (erect posture, EP; forward trunk bending, FTB) were carried out during four months of microgravity exposure. Results show that postural strategies for quasistatic body orientation in weightlessness are based on the alignment of geometrical body axes (head and trunk) along external references. A proper whole body positioning appears to be recovered only after months of microgravity exposure. By contrast, typically, terrestrial strategies of co-ordination between movement and posture are promptly restored and used when performing motor activities in the weightless environment. This result is explained under the assumption that there may be different sensorimotor integration processes for static and dynamic postural function and that the organisation of coordinated movement might rely stably on egocentric references and kinematics synergies for motor control.     

The influence of microgravity on invasive growth in Saccharomyces cerevisiae

This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Σ1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Σ1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Σ1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.     

Detection of the quantity of kinesin and microgravity-sensitive kinesin genes in rat bone marrow stromal cells grown in a simulated microgravity environment

Kinesin and kinesin-like proteins (KLPs) constitute a superfamily of microtubule motor proteins found in all eukaryotic organisms. Members of the kinesin superfamily are known to play important roles in many fundamental cellular and developmental processes. To date, few published studies have reported on the effects of microgravity on kinesin expression. In this paper, we describe the expression pattern and microgravity-sensitive genes of kinesin in rat bone marrow stromal cells cultured in a ground-based rotating bioreactor. The quantity of kinesin under the clinorotation condition was examined by immunoblot analysis with anti-kinesin. Furthermore, the distribution of kinesin at various times during clinorotation was determined by dual immunostaining, using anti-kinesin monoclonal antibody or anti-β-tubulin monoclonal antibody. In terms of kinesin quantity, we found that the ratios of the amounts of clinorotated/stationary KLPs decreased from clinorotation day 5 to day 10, although it increased on days 2 and 3. Immunofluorescence analysis revealed that kinesin in the nucleus was the first to be affected by simulated microgravity, following the kinesin at the periphery that was affected at various times during clinorotation. Real-time RT-PCR analysis of kinesin mRNA expression was performed and led to the identification of 3 microgravity-sensitive kinesin genes: KIF9, KIFC1, and KIF21A. Our results suggest that kinesin has a distinct expression pattern, and the identification of microgravity-sensitive kinesin genes offers insight into fundamental cell biology.     

Autoignition of fuel-oxidizer mixtures in microgravity

The paper examines the limitations of quasi-steady autoignition theory in providing information needed for the characterization of autoignition temperature fields. Time-dependent autoignition theory is then utilized to examine the autoignition behavior of a reactive system where both wall catalysis and gas phase kinetic rates are significant. It is found that a diverse range of space-time temperature-composition-pressure histories is possible, depending on the system's thermo-kinetic characteristics.     

Some aspects of continuous flow electrophoresis in microgravity

Zone electrophoresis is a highly efficient method of separating biological products. It is based on the differences of mobilities of ionized particles in an electric field. During the separation complications arise due to electro-osmosis or thermal convection generated by Joule heating.This paper analyses the hydrodynamical running of a continuous flow zone electrophoresis cell and shows the influence of specific parameters on the separation.The modelling of the flow structure of the buffer solution is developed. The equations and the corresponding boundary conditions are solved by finite difference method. The model established provides knowledge of the hydrodynamical perturbations generated by electro-osmosis and thermal free convection. The case of microgravity is considered.     

Quantitative analysis of motion control in long term microgravity

In the frame of the 179-days EUROMIR '95 space mission, two in-flight experiments have foreseen quantitative three-dimensional human movement analysis in microgravity. For this aim, a space qualified opto-electronic motion analyser based on passive markers has been installed onboard the Russian Space Station MIR and 8 in flight sessions have been performed. Techhology and method for the collection of kinematics data are described, evaluating the accuracy in three-dimensional marker localisation. Results confirm the suitability of opto-electronic technology for quantitative human motion analysis on orbital modules and raise a set of "lessons learned", leading to the improvement of motion analyser performance with a contemporary swiftness of the on-board operations. Among the experimental program of T4, results of three voluntary posture perturbation protocols are described. The analysis suggests that a short term reinterpretation of proprioceptive information and re-calibration of sensorimotor mechanisms seem to end within the first weeks of flight, while a continuous long term adaptation process allows the refinement of motor performance, in the frame of never abandoned terrestrial strategies.     

Body orientation and center of mass control in microgravity

The control of the body orientation and the center of mass position with respect to the feet was investigated under normo- and microgravity (space flight Altair), during erect posture and at the end of a forward or backward upper trunk movement.

It was observed that during erect posture, the trunk orientation with respect to the vertical was inclined some 6 ° forward in both subjects under microgravity, whereas it was vertical or slightly backward oriented under normogravity. Under microgravity, on the contrary, the initial position CM changed either backwards or forwards. This result suggests that the inclined trunk posture might be due to misevaluating the vertically under microgravity and that different control mechanisms are involved in orienting the trunk and placing the CM.

It was also noted that the final position of the CM at the end of the movement did not differ markedly between microgravity and normogravity. This result suggests that the kinematic synergies which stabilize the CM during uppertrunk movements may result from an automatic central control which is independent from the gravity constraints.     

Altered expression of mRNAs implicated in osteogenesis under conditions of simulated microgravity is regulated by CD200:CD200R

Mouse calvarial cells grown under simulated microgravity conditions (neutral buoyancy) show preferential differentiation towards the osteoclast lineage, as defined by surrogate mRNAs, bone nodule growth and TRAP⁺ cells, when compared with cells cultured under normal gravity conditions. This effect was suppressed in cultures which contained the immunoregulatory molecule CD200, and conversely enhanced by anti-CD200 mAb. Concomitant increases occur in expression of inflammatory cytokines, and their mRNAs, under simulated microgravity conditions. Again cultures containing exogenous CD200 showed suppressed cytokine and cytokine mRNA expression. Further alterations in osteoclastogenesis were seen using cells isolated from cytokine-receptor knockout mice. We conclude that, as assessed by altered expression of mRNAs associated with osteoblast differentiation, CD200:CD200R interactions play an important regulatory role in the enhanced osteoclastogenesis seen under simulated microgravity conditions, with changes in cytokine expression further modulating this effect.     

Crystallization of metal alloys in microgravity and uniform compression conditions

This is an analysis of the experimental results to study the metastable phase formation while crystallization of binary metal melts in microgravity and quasi-isostatic (uniform) compression takes place. The alloy system PbSn serves as an illustration     

Effect of enclosure shape on natural convection velocities in microgravity

A numerical analysis was performed to compare natural convection velocities in two-dimensional enclosures of various shape. The following shapes were investigated: circle, square, horizontal and upright 2 × 1 aspect ratio rectangles, horizontal and upright half-circles, diamond (square oriented with diagonal vertical) and triangle (equilateral and horizontal base). In all cases, the length scale in the various dimensionless parameters, such as Rayleigh number, is defined as the diameter of the equal area circle. Natural convection velocities were calculated for Rayleigh numbers of 100 and 500 with the temperature difference taken to be across (a) the maximum horizontal dimension, (b) the median horizontal line (line through centroid) and (c) the horizontal distance such that the temperature gradient is the same for shapes of equal area. A Rayleigh number of 1000 is within the “low Rayleigh number” range for agreement with first order theory for circular enclosures. A Rayleigh number of 5000 is slightly out of this range. For the class of shapes including the square, upright half-circle and upright rectangle, the computed velocities were found to agree very closely with that of the equal area circle when the temperature difference is taken to be across the maximum horizontal dimension [condition (a)]. The velocities for the horizontal rectangle and half circle were found to be approximately one-half that of the equal area circle for the same condition. Better overall agreement among all shapes was obtained by setting the temperature difference across a distance such that the temperature gradients were equal for shapes of equal area.     

The role of cytoskeleton in cell changes under condition of simulated microgravity

Single cells and cell culture are very good model for estimation of primary effects of gravitational changes. It is suggested that cell cytoskeleton plays a key role in mechanisms of adaptation to mechanical influences including gravitational ones. Our results demonstrated that cultured cells of human vascular endothelium (correction of endotheliun) are highly sensitive to hypogravity (clinorotation) and respond by significant decrease of cell proliferative activity. Simultaneously it was noted that the formation of confluent monolayer appeared early in cultures exposed to simulated microgravity due to accelerated cells spreading. Long-term hypogravity (several hours or days) leads to significant changes of cell cytoskeleton revealed as microfilament thinning and their redistribution within cell. Such changes were observed only in monolayer cells and not in cell suspensions. Gravitational forces as known to be modificators of cell adhesive ability and determine their mobility. Hypogravity environment stimulated endothelial cell migration in culture: 24-48 hrs pre-exposition to hypogravity significantly increased endothelial cell migration resulting in 2-3-fold acceleration of mechanically injured monolayer repair. Obtained results suggest that the effects of hypogravity on cultured human endothelial cells are, possibly, associated with protein kinase C and/or adenylate cyclase activity and are accompanied by noticeable functional cell changes.     

Eddy currents induced migration of droplets in electrically conducting fluid in microgravity environments

Electrical eddy currents induced migration of droplets, whose conductivity differs from that of surrounding fluid, is analysed. Eddy currents in a dilute suspension under consideration, which fills the dielectric tube, are induced by alternating magnetic field parallel to the tube axis. In the case where the droplet radius is small as compared to the skin depth, the distributions of magnetic fields, currents and electromagnetic forces inside and outside the droplet have been calculated. For small Reynolds numbers, with neglecting inertial and thermocapillary effects and the gravity, the flow of fluids around the droplet and inside it, caused by electromagnetic forces, has been studied. The formula is obtained, which relates the droplet migration velocity to the electro-magnetic field, the droplet size and physical properties of fluids.     

A review of muscle atrophy in microgravity and during prolonged bed rest

With the prospect of long duration space missions in Earth orbit or to Mars, there is a need for adequate information on the physiological adaptations that will occur. One consequence of prolonged exposure to microgravity is muscle atrophy (loss of muscle mass). After a long duration space flight, muscle atrophy along with skeletal calcium loss would affect the capacity of astronauts to re-adapt to gravity on return to Earth. Of importance are any countermeasures which can attenuate the adaptive responses to microgravity. Experimentation is difficult in space with small subject numbers and mission constraints. Prolonged bed rest using healthy volunteers is used as an Earth-based model to simulate the muscle atrophy which occurs in the microgravity environment.     

Mental representation of spatial cues in microgravity: Writing and drawing tests

Humans have mental representation of their environment based on sensory information and experience. A series of experiments has been designed to allow the identification of disturbances in the mental representation of three-dimensional space during space flight as a consequence of the absence of the gravitational frame of reference. This NASA/ESA-funded research effort includes motor tests complemented by psychophysics measurements, designed to distinguish the effects of cognitive versus perceptual-motor changes due to microgravity exposure. Preliminary results have been obtained during the microgravity phase of parabolic flight. These results indicate that the vertical height of handwritten characters and drawn objects is reduced in microgravity compared to normal gravity, suggesting that the mental representation of the height of objects and the environment change during short-term microgravity. Identifying lasting abnormalities in the mental representation of spatial cues will establish the scientific and technical foundation for development of preflight and in-flight training and rehabilitative schemes, enhancing astronaut performance of perceptual-motor tasks, for example, interaction with robotic systems during exploration-class missions.     

Effects of microgravity on muscular explosive power of the lower limbs in humans

The maximal explosive power of the lower limbs of one astronaut has been measured before launch, and 2, 6 and 11 days after re-entry from 31 days on the MIR Station (EUROMIR '94). The subject, sitting on the carriage-seat of a Multipurpose Ergometer-Dynamometer (MED) constructed ad hoc in our laboratory, pushed maximally with both feet on two force platforms (knees angle 110 degrees). The carriage was free to move backwards on two rails inclined 20 degrees upwards. The force (F) of the lower limbs and the speed of the carriage (v) were recorded and the instantaneous mechanical power (w) was calculated as w = F * v. The average value of the mechanical power (w max) throughout the explosive effort was then obtained. The overall duration of the push was on the average about 0.3 s. It was observed that, at day R+2, mean force, maximal velocity, maximal power (mean and peak), maximal acceleration and overall mechanical work, were all reduced between 60 and 80% of pre-flight values. However, the recovery was remarkably fast, since all these parameters attained about 90% of pre-flight values by day R+11.     

The asymmetrical growth of otoliths in fish is affected by hypergravity.

Size and asymmetry (size difference between the left and the right side) of inner ear otoliths of larval cichlid fish were determined after a long-term stay at moderate hypergravity conditions (3g; centrifuge), in the course of which the animals completed their ontogenetic development from hatch to freely swimming. Both the normal morphogenetic development as well as the timely onset and gain of performance of the swimming behaviour was not impaired by the experimental conditions. However, both utricular and saccular otoliths (lapilli and sagittae, respectively) were significantly smaller after hyper-g exposure as compared to parallely raised 1g control specimens. The asymmetry of sagittae was significantly increased in the experimental animals, whereas the respective asymmetry con-cerning lapilli was pronouncedly decreased in comparison to the 1g controls. These findings suggest, that the growth and the development of bilateral asymmetry of otoliths is guided by the environmental gravity vector.     

滑动聚束SAR空变天线方向图校正方法

滑动聚束SAR是一种新型高分辨率星载SAR工作模式,通过天线波束扫描导致增加方位向相干积累时间,从而可以大幅提升SAR分辨率.滑动聚束SAR波束扫描将引起天线方向图加权二维空变,进而导致采用传统SAR天线方向图测量和校正方法会引入误差,影响SAR辐射定标精度及目标后向散射系数测量精度.针对此问题,提出了一种滑动聚束SAR天线方向图特性分析及校正方法.首先基于滑动聚束SAR仿真数据对滑动聚束模式下SAR天线方向图二维空变特性进行了分析;然后建立距离向和方位向二维多项式模型来拟合天线方向图二维空变误差;最后,基于天线方向图空变误差模型对观测场景SAR图像进行天线方向图校正,仿真实验结果表明,该方法可以有效降低天线方向图二维空变误差的影响,大大提高滑动聚束SAR辐射定标精度和目标后向散射系数测量精度.     

微重力环境下推进剂贮箱中三维气液平衡界面的数值模拟

以部分管理表面张力贮箱的管理舱为研究对象,利用三维气液平衡界面计算程序Surface Evolver,在无重力和微重力且几何边界条件比较复杂的环境下对管理舱内的气液平衡界面进行数值模拟;计算结果与已经应用卫星的理论计算完全吻合。     

基于FDS的微重力条件下密闭舱细水雾灭火过程仿真分析

文章应用火灾动力学软件(FDS)对微重力条件下载人航天器密闭舱细水雾灭火过程进行仿真分析,得到了电缆火灾扑灭过程中舱内温度和CO的分布规律。结果表明,一定倾斜角度喷射细水雾不但会促进烟雾的扩散,还会为燃烧增加新鲜空气,使扑灭过程趋于困难。分析认为,微重力环境中的细水雾灭火器喷雾粒径应控制在100~150μm,且使用时应使喷雾方向与火灾面尽量趋于垂直。