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根据鸡传染性支气管炎病毒M41株的基因序列(GenBank:AY851295.1),设计特异性引物,利用 RT-PCR方法扩增其 S1基因,并将扩增产物克隆到杆状病毒转移质粒pFast-VSV-G-CMV中,获得重组质粒pFast-VSV-G-CMV-S1,进一步将该重组质粒转化到DH10Bac感受态细胞中,得到重组穿梭载体Bacmid-CMV-S1,再利用脂质体转染sf9昆虫细胞,获得重组杆状病毒Ac-V-S1.间接免疫荧光试验表明,该重组杆状病毒可以转导哺乳动物细胞并表达S1蛋白. 相似文献
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介绍了GPS P码直接捕获方法的性能,详细论述扩展复制重叠法的原理和性能,从理论上证明扩展复制重叠法是目前技术条件下行之有效的GPS P码直接捕获方法,通过实例验证了理论的正确性。 相似文献
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前言离子镀膜作为一种镀膜新工艺在国外得到了广泛的运用。它已被用于耐腐、耐热、耐磨装饰等方面,以及机械、光学和电子工业中。利用离子镀可以镀多种金属、氧化物、硫化物、塑料、玻璃、陶瓷等材料。离子镀膜为渗金属开辟了一条新路。目前 相似文献
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从GenBank上下载所有1978-2009年在中国分离的H1N1亚型流感病毒的HA和NA基因及所有同期在中国分离的A型流感病毒(宿主包括人、猪和禽)的PB1、PB2、PA、NS、NP和M基因的序列,将这些基因序列与2009年在中国发生的墨西哥H1N1流感病毒的对应基因序列进行BMCMC法分析,绘制BMCMC进化树,结果揭示中国发生的墨西哥H1N1流感病毒的HA和NA基因相对独立于中国季节性H1N1流感病毒;M基因相对独立于中国国内的季节性流感病毒和禽源流感病毒;PB1基因包含在季节性流感病毒的分群内;PB2基因和NS基因与国内重排的流感病毒相关,但相对独立于国内的季节性流感病毒和禽源流感病毒;PA基因包含在国内禽流感病毒的分群内;NP基因则与国内传统的猪流感病毒相关,但相对独立于国内的季节性流感病毒和禽源流感病毒. 相似文献
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信息战的定义及基本内容美国曾于1992年给信息战下过一个定义,但这个定义是保密的,据悉美国目前正在对这一定义作进一步的修订。目前广泛使用的是信息战的一个功能上的定义,即信息战是对敌方的信息、以信息为基础的各种过程和信息系统施加影响,同时保护己方的信息、以信息为基础的各种过程和信息系统,最终取得“制信息权”的一切可能的行为。由这个定义可以看到信息战所涉及的范围之广,可以说无所不包,一切与信息有关的事物都可能被纳入信息战的范畴。广义的信息战存在于和平时期、危机时期和战争时期,可以公开地也可以秘密地进行。… 相似文献
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随着计算机的广泛应用,工程绘图正在由传统的图板绘图向计算机绘图转变。这种情况的出现是由于计算机绘图奉统比传统的图板绘图方法能给人们提供更多的优点:计算机绘图快,可以进行较多的连续绘图,并且可以直接与生产操作机器如计算机数字控制机床(CNC)相连接。由计算机绘制的图样可以贮存在计算机的内存中并且可以修改,还可以迅速、准确地复制出来,零件明细表的信息可以被贮存起来并加以处理,因此可以提 相似文献
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目前GPS 系统广泛应用于定位、测速中,尽管其在低速、低过载的相关领域
中没有限制,但在一些特殊环境,例如火箭橇试验的大过载环境中,可能因国外技术限制
无法正常工作。而北斗导航系统作为我国自主开发的系统则不会受到此类制约。首先介绍
了卫星导航定位理论,从理论上分析了卫星导航系统工作不受到载体速度和加速度限制。
之后,通过两次试验,发现了GPS 系统受到限制的情况。最后,通过比较GPS 系统和北斗
导航系统的定位、测速精度,得到了可以使用北斗导航系统替代GPS 系统的结论。 相似文献
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一、信息与民航信息“信息”一词(Information),屡见不鲜。1992年8月国家科委明确规定“科技情报”应改称“科技信息”,从而使“信息”这个概念取代“情报”更被广泛使用。信息是客观事物某种属性的反映。信息不是事物本身,而是被反映物的属性再现,故而信息离不开载体和传递。80年代以来,信息已经成为西方发达国家重点发展的产业,他们纷纷拟订对策,加紧信息资源的开发、利用,加快信息产业和高新技术产业的 相似文献
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表贴式永磁同步电机直接转矩控制变幅值预测控制研究 总被引:2,自引:3,他引:2
基于以电压矢量幅值为变量的表贴式永磁同步电机(SPMSM)磁链和转矩方程,采用预测控制计算得出施加不同电压矢量幅值的下一时刻磁链和转矩值,选择使磁链和转矩误差目标函数最小的电压矢量幅值作为最优值,从而确定下一时刻施加的电压矢量。仿真结果表明:在电压矢量变幅值预测下,SPMSM直接转矩控制(DTC)系统运行良好,定子磁链轨迹为理想圆,磁链和转矩均符合控制要求,转速跟踪良好,定子电流波形正弦。预测控制需要从备选电压矢量幅值集合中选择最优幅值。理论上,备选电压矢量幅值个数越多,系统优化效果越好,但也带来更大的计算负担。研究了9种等分电压矢量情况下变幅值预测控制系统的控制效果。根据控制效果和计算负担,提出变幅值预测控制将电压矢量幅值三等分较为理想。 相似文献
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A Fast Beamforming Algorithm for Large Arrays 总被引:3,自引:0,他引:3
This beamforming algorithm is written specifically for array radars in which the number of array elements K is very large compared with the number of jammers L the radar is designed to suppress. It uses a set of M noise vectors to construct a basis for the jammer component of the antenna output vectors. The component of the quiescent weight vector orthogonal to each basis vector is calculated, renormalized to unit length, and identified as the adapted weight vector. This algorithm is effective in the suppression of many types of jammers. The number of noise samples M required in the construction of the adapted weight vector is approximately equal to L. In the special case of L narrowband noise jammers, for example, a choice of M = L usually reduces the receiver output jammer power to a few dBs above the white noise background. It is permissible to have M相似文献
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针对机载多输入多输出(MIMO)雷达空时自适应处理(STAP)中期望目标导向矢量的失配问题,提出一种基于三迭代(TRIA)与二阶锥规划(SOCP)的稳健降维MIMO-STAP方法。首先将MIMO-STAP权矢量分解为发射、接收、多普勒3个低维权矢量的Kronecker积,然后分别限定实际发射、接收、多普勒导向矢量与假定导向矢量之间的误差范数边界,通过优化最差性能,利用SOCP对各个低维权矢量进行三迭代求解,最后进行权矢量合成。该方法在保证机载MIMO雷达获取稳健STAP性能的同时,通过三迭代降维处理,能够有效降低训练样本数需求与运算复杂度,因此更具有实际应用价值。仿真结果验证了算法的有效性。 相似文献
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Bharadwaj P. Runkle P. Carin L. Berrie J.A. Hughes J.A. 《IEEE transactions on aerospace and electronic systems》2001,37(2):595-606
Wideband electromagnetic fields scattered from N distinct target-sensor orientations are employed for classification of airborne targets. Each of the scattered waveforms is parsed via physics-based matching pursuits, yielding N feature vectors. The feature vectors are submitted to a hidden Markov model (HMM), each state of which is characterized by a set of target-sensor orientations over which the associated feature vectors are relatively stationary. The N feature vectors extracted from the multiaspect scattering data implicitly sample N states of the target (some states may be sampled more than once), with the state sequence modeled statistically as a Markov process, resulting in an HMM due to the “hidden” or unknown target orientation. In the work presented here, the state-dependent probability of observing a given feature vector is modeled via physics-motivated linear distributions, in lieu of the traditional Gaussian mixtures applied in classical HMMs. Further, we develop a scheme that yields autonomous definitions for the aspect-dependent HMM states. The paradigm is applied to synthetic scattering data for two simple targets 相似文献
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Raghavan R.S. Qiu H.F. McLaughlin D.J. 《IEEE transactions on aerospace and electronic systems》1995,31(2):647-657
We develop a constant false-alarm rate (CFAR) approach for detecting a random N-dimensional complex vector in the presence of clutter or interference modeled as a zero mean complex Gaussian vector whose correlation properties are not known to the receiver. It is assumed that estimates of the correlation properties of the clutter/interference may be obtained independently by processing the received vectors from a set of reference cells. We characterize the detection performance of this algorithm when the signal to be detected is modeled as a zero-mean complex Gaussian random vector with unknown correlation matrix. Results show that for a prescribed false alarm probability and a given signal-to-clutter ratio (to be defined in the text), the detectability of Gaussian random signals depends on the eigenvalues of the matrix Rc-1Rs. The nonsingular matrix Rc and the matrix Rs are the correlation matrices of clutter-plus-noise and signal vectors respectively. It is shown that the “effective” fluctuation statistics of the signal to be detected is determined completely by the eigenvalues of the matrix Rc-1Rs. For example the signal to be detected has an effective Swerling II fluctuation statistics when all eigenvalues of the above matrix are equal. Swerling I fluctuation statistics results effectively when all eigenvalues except one are equal to zero. Eigenvalue distributions between these two limiting cases correspond to fluctuation statistics that lie between Swerling I and II models 相似文献
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A technique is described for obstacle detection, based on the expansion of the image-plane projection of a textured object, as its distance from the sensor decreases. Information is conveyed by vectors whose components represent first-order temporal and spatial derivatives of the image intensity, which are related to the time to collision through the local divergence. Such vectors may be characterized as patterns corresponding to “safe” or “dangerous” situations. We show that the essential information is conveyed by single-bit vector components, representing the signs of the relevant derivatives. We use two recently developed, high capacity classifiers, employing neural learning techniques, to recognize the imminence of collision from such patterns 相似文献
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Steered beam adaptive arrays for multiple simultaneous desired signals are discussed. It is shown that the performance of a steered beam adaptive array depends upon the range of input signal strengths and the choice of the steering vector. Optimum steering vectors for various input signal strengths are given. All choices of steering vectors are equally effective in the rejection of jammers. 相似文献