Urodelean amphibians in studies on microgravity: effects upon organ and tissue regeneration.

Results obtained from nine experiments performed onboard Russian biosatellites have shown that microgravity promotes tissue regeneration in the newt, Pleurodeles waltl. The effect has been reproduced in all flights and on a clinostat as well for eye tissues (lens and retina), limbs and tail. The effect was demonstrated in 1.5- to 2-fold increase in cell proliferation in the early stages of regeneration in space flight. Animals "flown" intact and operated after flight regenerated faster than control ones and showed long-lasting micro-"g" effect. The most recent experiment flew aboard the Bion-11 biosatellite. This test was performed for study on microgravity effect on neural retina regeneration after optic nerve lesioning in the newt. Obtained results confirmed our previous information about intensification of regenerative processes in detached neural retina in urodela exposed to simulated weightlessness (Grigoryan et al., 1998). In particular, we found the increase and activation of cell populations participating in neural retina restoration and maintenance of retinal structure. Our findings suggest that promoting effect of microgravity upon regeneration could be influenced by several factors, largely influenced by a response of the whole organism to changed gravity vector. We hypothesized the synthesis of the specific range of stress proteins induced by micro-"g" and their regulative role in cell proliferation. Such a hypothesis for the existence of "altered gravity stress proteins" is discussed.     

Cell fusion in space: plasma membrane fusion in human fibroblasts during short term microgravity.

During short-term microgravity in sounding rocket experiments (6 min.) the cytoskeleton undergoes changes and therefore it is possible that cell processes which are dependent on the structure and function of the cytoskeleton are influenced. A cell fusion experiment, initiated by a short electric pulse, was chosen as a model experiment for this sounding rocket experiment. Confluent monolayers of primary human skin fibroblasts, grown on coverslips, were mounted between two electrodes (distance 0.5 cm) and fused by discharging a capacitor (68 micro F; 250 V; 10 msec) in a low conductive medium. During a microgravity experiment in which nearly all the requirements for an optimal result were met (only the recovery of the payload was delayed) results were found that indicated that microgravity during 6 minutes did not influence cell fusion since the percentage of fused products did not change during microgravity. Within the limits of discrimination using morphological assays microgravity has no influence on the actin/cortical cytoskeleton just after electrofusion.     

锂离子电池热失控气体燃烧对热失控传播影响的量化方法

为量化锂离子电池热失控过程产生热失控气体对热失控传播的影响,基于能量守恒方程和铜基电池量热法,提出了一种计算热失控气体燃烧对热失控传播贡献占比的方法。选取商用18650型电池,利用自主设计搭建的热失控气体释能测算实验平台获取计算所需参数。实验结果表明：第1节电池热失控气体燃烧释放的能量在第2节电池热失控所需能量中占比达到5.42%,使第2节电池自产热增加了42%,热失控时间提前了29%。研究结果有助于进一步探索热失控传播过程中的能量传递效率,为单元层级和系统层级的电池安全设计提供理论支持。     

The white blood cell line: changes induced in mice by hypergravity.

The effect of hypergravity on the white blood cell (WBC) line of mice was investigated by use of horizontal centrifuge. Several sets of experiments were performed, in which the parameters measured were the WBC and differential cell count in the peripheral blood. In another experiment, lymphocyte counts from the spleen, lymph nodes, and the thymus were measured. The needed samples were taken from the mice during a stay of 7-40 days under a hypergravity of 1.6G. The test groups that were placed on the arms of the centrifuge (1.6G) were compared with stationary control groups (1G) and a rotating control group located at the center of the centrifuge (1G). Such a comparison revealed the test animals to be deficient on all counts, to wit, showing a decrease in total number of WBC's, a decrease in lymphocyte number in the peripheral blood and a decrease in the number of lymphocyte in the spleen and thymus. The decrease of lymphocytes in peripheral blood was characterized by two different slopes--an early and temporary decrease at the first days of the experiment evident in both test and rotating control groups followed by a temporary increase, and a later persistent decrease, evident only in the test group, while in the rotating control lymphocyte counts reverted to normal. There were no significant differences in monocyte or neutrophil counts, except for a temporary increase in the number of neutrophils which peaked on the seventh day. In order to evaluate the effect of hypergravity on restoration of hematopoiesis following hematopoietic suppression, 5-fluoro-uracil (5-FU) was administered i.v. to both the experimental and control mice. Suppression of bone marrow was observed in all groups injected with 5-FU, but while there was later an increase in cell counts in the control groups, there was no such increase in the test group subjected to hypergravity.     

APS星敏感器太阳照射影响分析及试验验证

针对APS星敏感器在轨过程中太阳照射问题进行了研究,分析了太阳照射对星敏感器功能和性能的影响,设计了太阳照射相关的试验及验证方案,最后给出了相关试验过程和试验验证结果,明确了分析及试验结论.通过分析和试验结果表明:紫外辐照试验对光学系统透过率无明显影响,且影响在APS星敏感器灵敏度余量范围内;在轨太阳照射对APS星敏感器探测器及整机的功能和性能无影响.相关分析和试验数据可作为星敏感器后续改进设计的依据和参考.     

The effect of microgravity on the development of plant protoplasts flown on Biokosmos 9.

An experiment using plant protoplasts has been accepted for the IML-1 Space Shuttle mission scheduled for 1991. Preparatory experiments have been performed using both fast and slow rotating clinostats and in orbit to study the effect of simulated and real weightlessness on protoplast regeneration. Late access to the space vehicles before launch has required special attention since it is important to delay cell wall regeneration until the samples are in orbit. On a flight on Biokosmos 9 ("Kosmos-2044") in September 1989 some preliminary results were obtained. Compared to the ground control, the growth of both carrot and rapeseed protoplasts was decreased by 18% and 44% respectively, after 14 days in orbit. The results also indicated that there is less cell wall regeneration under micro-g conditions. Compared to the ground controls the production of cellulose in rapeseed and carrot flight samples was only 46% and 29% respectively. The production of hemicellulose in the flight samples was 63% and 67% respectively of that of the ground controls. In both cases all samples reached the stage of callus development. The peroxidase activity was also found to be lower in the flight samples than in the ground controls, and the number of different isoenzymes was decreased in the flight samples. In general, the regeneration processes were retarded in the flight samples with respect to the ground controls. From a simulation experiment for IML-1 performed in January 1990 at ESTEC, Holland, regenerated plants have been obtained. These results are discussed and compared to the results obtained on Biokosmos 9. Protoplast regeneration did not develop beyond the callus stage in either the flight or the ground control samples from the Biokosmos 9 experiment.     

空间太阳电池板银互连片原子氧效应模拟试验

空间太阳电池板可作为空间飞行器的动力源,但由于原子氧在空间太阳电池板银互连片上的腐蚀作用,可能影响空间太阳电池板的有效寿命.因此,在北航流体所设计的原子氧效应地面模拟试验设备中,对空间太阳电池板的银互连片进行了抗原子氧效应地面模拟试验研究.获得了银箔和不同防护镀层材料在原子氧环境中的不同剥蚀结果,这为进行银互连片表面的原子氧防护提供了必要的应用和设计依据.     

Space environmental factors affecting responses to radiation at the cellular level.

Previous space experiments suggest a high value for the RBE of cosmic radiation. A possible explanation could be a change in cell radiosensitivity due to a combined effect of radiation and other factors related to the space environment and to the space flight. Results of the EXOBLOC II experiment support this assumption. On earth, vibrations or accelerations applied before or after irradiation can change the responses to radiation. Microgravity could be the main factor affecting the radiosensitivity and DNA repair but this hypothesis must be confirmed by additional experiments.     

Longevity in space; Experiment on the life span of Paramecium cell clone in space

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.     

松质骨微观骨小梁结构的生物力学综合分析

股骨松质骨具有骨小梁的微观结构特性,影响了股骨的整体力学性能,找到适当的单元形状与骨小梁分布特点可以有效地进行股骨生物力学特性分析．采用不同结构的微观骨小梁单元模型分析各单元的生物力学特性,并采用快速成形样件力学实验和有限元分析相结合的方法,对由不同骨小梁单元及不同分布形式构成的股骨模型进行生物力学分析,找到了较好的微观骨小梁单元模型及总体布局形式,获得了快速成形样件实验分析与有限元分析2种结果的综合分析,并应用于股骨松质骨建模．所获得的微观单元优化结构可以有效地应用于仿生结构材料的制造．      

Longevity in space; experiment on the life span of Paramecium cell clone in space.

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.     

Preliminary results of space experiment: Implications for the effects of space radiation and microgravity on survival and mutation induction in human cells

In view of the concern for the health of astronauts that may one day journey to Mars or the Moon, we investigated the effect that space radiation and microgravity might have on DNA damage and repair. We sent frozen human lymphoblastoid TK6 cells to the International Space Station where they were maintained under frozen conditions during a 134-day mission (14 November 2008 to 28 March 2009) except for an incubation period of 8 days under 1G or μG conditions in a CO₂ incubator. The incubation period started after 100 days during which the cells had been exposed to 54 mSv of space radiation. The incubated cells were then refrozen, returned to Earth, and compared to ground control samples for the determination of the influence of microgravity on cell survival and mutation induction. The results for both varied from experiment to experiment, yielding a large SD, but the μG sample results differed significantly from the 1G sample results for each of 2 experiments, with the mean ratio of μG to 1G being 0.55 for the concentration of viable cells and 0.59 for the fraction of thymidine kinase deficient (TK⁻) mutants. Among the mutants, non-loss of zygosity events (point mutations) were less frequent (31%) after μG incubation than after 1G incubation, which might be explained by the influence of μG on cellular metabolic or physiological function. Additional experiments are needed to clarify the effect of μG interferes on DNA repair.     

Putative graviperception mechanisms of protists.

Many (if not all) free-living cells use the gravity vector for their spatial orientation (gravitaxis). Additional responses may include gravikinesis as well as changes in morphological and physiological parameters. Though using essentially different modes of locomotion, ameboid and ciliated cells seem to rely on common fundamental graviperception mechanisms. Uniquely in the ciliate family Loxodidae a specialized intracellular gravireceptor organelle has been developed, whereas in all other cells common cell structures seem to be responsible for gravisensing. Changes in direction or magnitude of acceleration (from 0 to 5 g) as well as experiments in density-adjusted media strongly indicate that either the whole cytoplasm or dense organelles like nuclei act as statoliths and open directly or via cytoskeletal elements mechano-sensitive ion channels in the cell membrane. A recent spaceflight experiment (S/MM-06) demonstrated that prolonged (9 d) actual weightlessness did not affect the ability of Loxodes to respond to acceleration stimuli. However, prolonged cooling (> or = l4 d, 4-10 degrees C) destroyed the ability for gravitactic orientation of Paramecium. This may reflect a profound effect either on the gravireceptor itself or on the gravity-signal processing. In gravity signalling the ubiquitous second messenger cAMP may be involved in acceleration-stimulus transduction.     

离子推力器羽流热效应仿真分析

离子推力器工作时向外喷出的羽流与航天器表面碰撞,会引起敏感材料热变形等热效应,严重时会导致航天任务失败。针对兰州空间技术物理研究所研制的LIPS-200型离子推力器羽流热效应进行了仿真分析。仿真中,使用粒子网格（PIC）方法处理等离子体运动,使用直接模拟蒙特卡罗（DSMC）方法处理粒子间碰撞,使用Maxwell模型处理粒子与壁面的能量交换,对电推进羽流热效应测量中的部分测点进行了数值模拟。结果表明,仿真结果与实验数据符合较好,离子推力器出口轴线上滞止热流仿真值与实验测量值误差小于17.0%。此外,热流计对流场的影响主要集中在热流计附近0.1 m范围内,对整体流场影响较小。      

重力对植物细胞生长的效应

从地基研究植物向重性和在神舟八号卫星微重力条件下培养植物细胞出发,探讨重力变化对植物细胞发生作用时产生的生物学效应.已有结果显示,在植物向重性反应和处于失重状态时,重力方向和大小变化对细胞壁代谢具有一定影响.推断细胞形状的维持是由细胞壁的刚性与细胞内膨压平衡所致,当细胞膨压大于细胞壁刚性导致上述平衡打破时就会引起细胞体积增大.因此,重力的变化可能会通过影响植物细胞壁刚性与细胞内膨压的平衡影响细胞生长.      

Intracellular molecular distributions in spacecraft experiments in orbit around Earth

It is possible that the nucleolous inside the cell plays the role of a “gravity receptor”. Furthermore, cells up to 10 μm in diameter can demonstrate some effect due to the redistribution of mitochondria or nucleolous. Effects of gravity should be present in various cell systems where larger objects such as the ribosomes move from cell to cell. In this paper we study the effects of gravity on cells. In particular, we examine the resulting intracellular molecular distribution due to Brownian motion and the ordered distribution of molecules under the action of gravity, where n₀ is the number per unit volume at certain level, and n is the number per unit volume above that level. This is an experiment that takes place at a certain orbital altitude in a spacecraft in orbit around Earth, where the acceleration due to the central field is corrected for the oblateness and also the rotation of the Earth. We found that equatorial circular and elliptical orbits have the highest n/n₀ ratios. This experiment takes place in circular and elliptical orbits, with eccentricities e = 0, 0.1 and involves a bacterial cell at an orbital altitude of 300 km. We found that n/n₀ = 1.00299 and 1.0037 respectively, which is still a 0.6–0.7 % higher than n/n₀ = 0.0996685 calculated on the surface of the Earth. Examining mitochondria in similar orbital experiments we found that equatorial orbits result to higher n/n₀ ratios. In particular, we found that n/n₀ = 8.38119, where an elliptical orbit of eccentricity e = 0.1 results to n/n₀ = 13.8525. Both are high above 100%, signifying the importance of Brownian motion over gravity. Our results are of interest to biomedical applications. Molecular concentrations are important for various processes such as the embryogenesis, positional homeostasis and its relation to cell energy expenditure, cell torque, cell deformation, and more. These results indicate that statistical molecular distributions play an important role for the recognition of a particular environment by the cell, in biological space experiment to come.     

原子自旋陀螺气室加热电磁噪声抑制实验研究

原子自旋陀螺仪作为目前最新一类陀螺仪,具有超高的理论精度。碱金属气室是原子自旋陀螺仪承载原子自旋的敏感表头。通过电加热使碱金属达到饱和蒸气压,但是电加热过程中会引入电磁干扰等噪声,进而影响原子自旋陀螺仪的精度和灵敏度。为减小碱金属气室加热的电磁噪声对原子自旋陀螺仪的影响,从加热器结构与加热驱动信号2个方面进行了电磁噪声抑制实验研究。设计了具有磁场噪声抑制作用的异形加热膜,使高频正弦波作为加热驱动信号,构建了碱金属气室集成化无磁电加热单元。通过实验验证,系统的等效磁场噪声优于17 fT/Hz1/2,气室内部的温度稳定度优于±0.006 ℃,为原子自旋陀螺仪的性能提升提供了可靠保障。      

Laboratory experiments on spacecraft charging and its neutralization

Simulation experiments on spacecraft charging in space plasma and its neutralization are performed in relation to the electron beam experiment (SEPAC) on Space Shuttle Spacelab 1. A spacecraft simulator or a spherical probe is immersed in a magnetized plasma and a positive high voltage with respect to the plasma is externally applied to it. The current-voltage characteristics follow quite well with the theoretical model of Parker and Murphy [1] in the low voltage, low pressure region. When the voltage rises to more than the ionization potential of the surrounding neutral gas, it departs from the model and the effect of plasma production by the electron current becomes very important. The same kind of ionization effect as this has also been observed in our rocket experiments with an electron beam. The enhancement of the ionization effect by an additional neutral gas injection causes a considerable suppression of the potential rise of a spacecraft emitting an electron beam. This is demonstrated with the SEPAC accelerators in a large space chamber experiment.     

空间对接机构碰撞试验系统的开发研究

为研究空间对接机构在对接过程中的碰撞现象,研制了碰撞试验系统.探讨了试验系统方案;依据Hertz弹性接触理论,仿真计算了加速度频率特性,确定了加速度传感器的选择原则;利用该系统开展了低速碰撞试验.试验结果表明,该系统能够满足测量对接碰撞力的要求.      

基于TCAD仿真建模的瞬时剂量率效应研究

针对一款百万门级0.18 μm互补金属氧化物半导体（0.18 μm CMOS）工艺抗辐射加固的微处理器电路,提出了采用TCAD仿真建模技术对加固电路开展瞬时剂量率效应研究的方法,仿真结果表明通过保护环结构加固设计,可有效提升电路的抗瞬时剂量率效应性能,并利用瞬时剂量率辐射源进行了仿真有效性的试验验证。结果表明,采用TCAD仿真手段得到的瞬时光电流峰值与试验得到的光电流峰值误差小于5%,证实了仿真研究电路瞬时剂量率效应方法的有效性。