Electric potentiation of gravikinesis in Paramecium is possibly mediated by filaments.

Sensitivity of Paramecium to mechanical stress including gravitational force is organized along two opposing gradients of membrane channel distribution: depolarizing Ca channels and hyperpolarizing K channels. Mechanoreceptor channels reside in the membrane of the cell soma and are activated, when the weight of the cytoplasm deforms the "lower" plasma membrane. Channel distribution is such as to generate ciliary activation which can counteract sedimentation of the cells: a reduction in downward swimming rate and an augmentation in upward swimming rate. Application of weak DC fields does not only induce the well-known cathodal orientation and swimming of Paramecium toward the cathode (galvano-taxis). We document that swimming velocity is augmented up to 175% as a function of the voltage gradient between 0.3 V/cm and 0.8 V/cm (galvanokinesis). A gradient of 0.3 V/cm was highly effective in raising the common negative gravikinesis of downward swimmers threefold. The gravikinesis of upward swimmers reversed polarity under field stimulation inducing cells to augment sedimentation effects (positive gravikinesis). Both effects of electric-field stimulation on ciliary activation are of the depolarizing type: reduction in the frequency of normally beating cilia. Analysis of the data shows that a voltage-sensitivity of gravireceptor channels would not account for the observed potentiation of negative gravikinesis. It is suggested that a previously described voltage-dependent Ca channel of the soma membrane interferes with a Ca(2+)-sensitive, peripheral filament system, which directly connects to gravireceptor channels.     

The identification of gravikinesis from ciliates: methods and experience.

Recent advances in the gravitational physiology of ciliates are reported: the theoretical and experimental assessment of gravikinesis and sedimentation, calculation of gravikinesis using slopes of observed swimming and sedimentation data under hypergravity, orientational distributions of gravikinesis, central and membrane-associated gravitransduction, and the kinetics of activation and relaxation of gravikinesis.     

Evaluation of gravity-dependent membrane potential shift in Paramecium.

It is still debated whether or not gravity can stimulate unicellular organisms. This question may be settled by revealing changes in the membrane potential in a manner depending on the gravitational forces imposed on the cell. We estimated the gravity-dependent membrane potential shift to be about 1 mV G⁻¹ for Paramecium showing gravikinesis at 1–5 G, on the basis of measurements of gravity-induced changes in active propulsion and those of propulsive velocity in solutions, in which the membrane potential has been measured electrophysiologically. The shift in membrane potential to this extent may occur from mechanoreceptive changes in K⁺ or Ca²⁺ conductance by about 1% and might be at the limit of electrophysiological measurement using membrane potential-sensitive dyes. Our measurements of propulsive velocity vs membrane potential also suggested that the reported propulsive force of Paramecium measured in a solution of graded densities with the aid of a video centrifuge microscope at 350 G was 11 times as large as that for −29 mV, i.e., the resting membrane potential at [K⁺]_o = 1 mM and [Ca²⁺]_o = 1 mM, and, by extrapolation, that Paramecium was hyperpolarized to −60 mV by gravity stimulation of 100- G equivalent, the value corrected by considering the reduction of density difference between the interior and exterior of the cell in the graded density solution. The estimated shift of the membrane potential from −29 mV to −60 mV by 100- G equivalent stimulation, i.e., 0.3 mV G⁻¹, could reach the magnitude entirely feasible to be measured more directly.     

Short-term responses of gravitaxis to altered gravity in Paramecium.

Negative gravitaxis of Paramecium almost disappeared in solutions having specific gravity about the same as that of the organisms (1.04). The taxis turned to positive in solutions of specific gravity 1.08. Using a drop shaft at the Japan Microgravity Center, Hokkaido (JAMIC) we examined how swimming behaviour in these media was modified by changing gravitational conditions before, during and after free-fall. Tracks of swimming cells recorded on videotape indicate that the swimming cells continued upward and downward shift depending on the specific gravity of the external medium under 1-g conditions and these vertical displacements disappeared immediately after the moment of launch. The effectiveness of changing gravity to induce displacement of the cells seems to depend on the orientation of the cells to gravity. These results suggest a corelation between vertical displacement of the cell through the medium and a gravitactic mechanism in Paramecium.     

Comparative studies of the graviresponses of Paramecium and Loxodes.

Gravitactic protozoa offer advantages in studying how the gravity stimulus is perceived on the cellular level. By means of a slow rotating centrifuge microscope in space the acceleration thresholds for gravitaxis of Loxodes striatus and Paramecium biaurelia were determined: < or = 0.15 x g for Loxodes and 0.3 x g for Paramecium, indicating different sensitivities of these species. Neutral-buoyant densities of immobilized cells were determined using media of different densities, revealing densities of 1.03 to 1.035 g/cm3 for Loxodes and 1.04 g/cm3 to 1.045 g/cm3 for Paramecium. Behavioral studies revealed that gravitaxis of Loxodes persisted independent of the density of the medium. In contrast, negative gravitaxis of Paramecium was no longer measurable if the density of the medium approached the density of the cell. The results suggest that in the case of Loxodes gravity is perceived by an intracellular receptor and, in the case of Paramecium by its own mass via the pressure on the lower cell membrane.     

Effects of gravity and cosmic rays on cell proliferation kinetics in Paramecium tetraurelia.

Space flights resulted in a stimulating effect on kinetics of proliferation in Paramecium tetraurelia. Additional experiments were performed in order to determine the origin of this phenomena. Paramecia were cultivated in balloon flights or in a slow clinostat, or were exposed to different levels of hypergravity. The results suggest that changes in cell proliferation rate are related to cosmic rays and to a direct effect of microgravity.     

Behavioural changes in Paramecium and Didinium exposed to short-term microgravity and hypergravity.

The swimming behaviour of two ciliate species, Paramecium caudatum and Didinium nasutum was analyzed under microgravity and hypergravity. In Paramecium the differences between former upward and downward swimming rates disappeared under weightlessness. At microgravity the swimming rates equalled those of horizontally swimming cells at 1g. In contrast, the swimming rates of Didinium increased under microgravity conditions, being larger than horizontal swimming rates at 1g. These findings are in accordance with a hypothesis of gravireception in ciliates based on electrophysiological data, which considers the different topology of mechanoreceptor channels in theses species. The hypothesis received further support by data recorded under hypergravity conditions.     

Longevity in space; experiment on the life span of Paramecium cell clone in space.

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.     

How gravity acts on Paramecium: new insights from free-fall experiments.

The swimming behaviour of Paramecium is affected by media of various specific gravities. At 1g, the negative gravitaxis of Paramecium virtually disappears in solutions the specific gravity of which is about the same as that of the organism (1.04). In solutions with a higher specific gravity (1.08), Paramecium becomes positively gravitactic. We recorded the swimming tracks of Paramecium in these media on videotape before, during and after free-falls. The records show that the density-dependent differences in the swimming behaviour disappeared immediately following the onset of the free-fall. The recorded tracks and distributions of cells in the experimental chambers were compared with computer-simulated traces and distributions based on gravitactic and gravikinetic models proposed for Paramecium. Our preliminary analysis favors a novel gravitactic mechanism involving modification of the ciliary movement The drop shaft at the Japan Microgravity Center, Hokkaido (JAMIC) was used for the free-fall experiments.     

A study of chemical systems using signal flow graph theory.

Photochemistry of giant planets and their satellites is characterized by numerous reactions involving a lot of chemical species. In the present paper, chemical systems are modeled by signal flow graphs. Such a technique evaluates the transmission of any input into the system (solar flux, electrons ... ) and gives access to the identification of the most important mechanisms in the chemical system. This method is applied to the production of hydrocarbons in the atmospheres of giant planets. In particular, the production of C2H6 in the atmosphere of Neptune from the photodissociation of CH4 is investigated. Different pathways of dissociation of CH4 are possible from L alpha radiation. A chemical system containing 14 species and 30 reactions including these different pathways of dissociation is integrated. The main mechanism of production of C2H6 is identified and evaluated for each model of dissociation. The importance of various reaction pathways as a function of time is presented.     

Statistical study of inverted-V events: A comparison between experiment and theory

We present a detailed study of the distribution and of the internal structure of the inverted-V electron precipitation commonly detected in the 500 – 2000 km altitude range aboard the AUREOL-3 satellite. These structured precipitations are statistically observed inside the auroral oval with a maximum occurence in the nightside sector. They correspond to primary electron fluxes peaked at energies generally below 10 keV. It is shown that, as predicted by kinetic theories, most inverted-V structures present a clear relationship between the field-aligned current density carried by the 1 – 20 keV primary electrons and the potential drop inferred from particle distribution functions. Furthermore the study demonstrates the existence of strong electron heating, related to the energy gain, when the current density exceeds some threshold of about 1 – 5 μA(m)^?2.     

Conjecture on superrotation in planetary atmospheres: A diffusion model with mixing length theory

Superrotation on Venus is discussed in the context of comparative planetary atmospheres. In our planetary system, the rigid shell component (global average) of superrotation is ubiquitous (Jupiter, Saturn, Earth, Venus, Mars, Titan). The largest equatorial values of the component are between 25 and 150 m/sec. We present a simplified, heuristic analysis, utilizing mixing length theory to describe the small scale non-linear advections of energy and angular momentum, thereby providing a closure of the dynamic system. This leads to the conjecture that the zonal velocity may be crudely estimated by

, approximating the observed planetary trends; with c the speed of sound, the parameter a being 1 or 2 for geostrophic or cyclostrophic conditions respectively, P_α an effective Prandtl number which becomes less than one when radiative cooling is important, S_o the average stability, Γ the adiabatic lapse rate and γ the ratio of specific heats.     

Kinetic theory of the current sheath. I. On polarization of an equilibrium current sheath

The basic theme of this paper is the investigation of the polarizing electric fields caused by anisotropy of thermal plasma of a current sheath. The paper provides a generalization of the equilibrium distribution function of the current sheath (Harris function) to the case of temperature anisotropy of plasma along and across the sheath. It appears that solution of the electroneutral equilibrium represents a narrow class of solutions and is true only for certain relations between the parameters of the problem. In the general case, the plasma of a stationary current sheath is polarized. The structure of the current sheath of a magnetosphere tail is investigated with regard to the effect of polarization, and profiles of the polarization electric fields are obtained. This field should be taken into account in the study of current sheath stability.     

A new calibration of the semi-empirical photometric theory for Halley and other comets

A massive new body of data, comprised of spectrophotometry of 17 comets by Newburn and Spinrad, has now become available for calibration of the basic theory, the Semi-Empirical Photometric Theory, used for modelling of Comet Halley. A redetermination of the constant $\text{R}$ and the function δ has been made, and no change is needed. Improved mixing ratios given as a function of heliocentric distance also permit an improvement in the constants of the visual photometric model presented at COSPAR XXIV. A new light curve for Halley and a lower dust to gas ratio prove to make roughly compensating changes in dust densities.     

Operation of magnetically assisted fluidized beds in microgravity and variable gravity: experiment and theory.

The conversion of solid waste into useful resources in support of long duration manned missions in space presents serious technological challenges. Several technologies, including supercritical water oxidation, microwave powered combustion and fluidized bed incineration, have been tested for the conversion of solid waste. However, none of these technologies are compatible with microgravity or hypogravity operating conditions. In this paper, we present the gradient magnetically assisted fluidized bed (G-MAFB) as a promising operating platform for fluidized bed operations in the space environment. Our experimental and theoretical work has resulted in both the development of a theoretical model based on fundamental principles for the design of the G-MAFB, and also the practical implementation of the G-MAFB in the filtration and destruction of solid biomass waste particles from liquid streams.     

Kinetic theory of the current sheath. II. Effect of polarization on the stability of a current sheath

A procedure for investigating the stability of a current sheath, taking into account the effect of plasma polarization, is offered. The kinetic equation with a self-consistent electromagnetic field for perturbation of the distribution function is solved. On the basis of this solution, the tensor of dielectric permeability for a non-electroneutral current sheath plasma is calculated, and the dispersion equation for the study of possible instability modes of this current sheath is obtained. The instability of the current sheath of the magnetospheric tail with respect to tearing perturbations, and the influence of the effect of plasma polarization on the development of tearing instability, are investigated.     

Kinetic theory of sheath formation in solar wind plasma

We present a general self-consistent kinetic theory for plasma sheath formation in solar wind plasma. The theory could be applied to anisotropic, as well as to isotropic collisionless plasma without resorting to any simplifications, limitations, or assumptions, such as the necessary existence of a ‘pre-sheath’ region of ions acceleration to ensure the Bohm criterion. The kinetic framework is first applied to sheath formation around an arbitrary oriented planar absorbing surface, charged by solar wind anisotropic plasma, under the condition of negligible photoelectric effect. We then make use of our kinetic approach for the plane geometry in isotropic collisionless plasma, as a particular case of a planar electrode orientation parallel to plasma streaming velocity, also analyzing the sheath structure around spherical and cylindrical absorbing electrodes submerged in isotropic collisionless plasma. Obtained results demonstrate principal differences in spatial charge distributions in sheath regions between spherical or cylindrical electrodes of large size and an unbound planar surface submerged in isotropic plasma. In the case of a planar electrode, we directly compare results obtained in our kinetic and hydrodynamic theories and conventional hydrodynamic theory of plasma sheath formation. The outcome from the present study have direct implications to the analysis of plasma sheath structure and associated distribution in space of charged dust grains, which is relevant to the moon exploration near the optical terminator region or in shadowed craters in the moon.     

Middle-atmosphere tides: Recent advances in theory and observation

Recent progress in the theoretical and observational investigation of middle atmosphere tides is reviewed. Theoretical advances include development of more realistic thermal excitation and numerical simulation models, investigation of seasonal — latitudinal and longitudinal variations in tidal structures, and the origin and effects of ‘unsteadiness’ in tidal oscillations over periods of days. Recent analyses of middle latitude radar data between 80 Km and 130 Km delineate consistent seasonal phase patterns in this region. Analyses of rocket data show stratosphere and lower mesosphere tides to be characterized by smaller amplitudes, shorter vertical scales, greater unsteadiness, and greater spatial variability than exhibited in the upper mesosphere and above. Results from the first two ATMAP (Atmospheric Tides Middle Atmosphere Program) campaigns are also discussed.     

Linear theory of steady X-point magnetic reconnection

Slow magnetic reconnection at a neutral X-point of a two-dimensional magnetic field is studied in an incompressible viscous resistive fluid. It is shown analytically that the combined effect of viscosity and resistivity resolves the current singularity appearing in both the ideal and resistive magnetohydrodynamic approximations at the X-point and along the separatrices when the flow is allowed to cross them. A previous attempt had retained a weak singularity at third order. A two-parameter family of exact solutions describing the structure of the flow and current density distribution is found for the corresponding basic equations.