Immune organs and haemopoietic system under modelling of the mission factors

Literary and experimental data on the character of changes in immune organs and lymphoid tissue of respiratory system and digestive system in laboratory animals during the mission factors model area given. Inhibition of reproductive function in bone marrow, thymus and spleen under irradiation of gamma-rays and accelerated carbon ions, tensity of immune response in the lymphoid structures of larynx, trachea and bronchi under the influence of acetaldehyde vapors and decrease of lymphoid tissue square on histological series in spleen and small intestine with an increase of concentration of microbial bodies in the drinking water were estimated.     

Tolerance of anhydrobiotic eggs of the Tardigrade Ramazzottius varieornatus to extreme environments

Tardigrades are tiny (less than 1?mm in length) invertebrate animals that have the potential to survive travel to other planets because of their tolerance to extreme environmental conditions by means of a dry ametabolic state called anhydrobiosis. While the tolerance of adult tardigrades to extreme environments has been reported, there are few reports on the tolerance of their eggs. We examined the ability of hydrated and anhydrobiotic eggs of the tardigrade Ramazzottius varieornatus to hatch after exposure to ionizing irradiation (helium ions), extremely low and high temperatures, and high vacuum. We previously reported that there was a similar pattern of tolerance against ionizing radiation between hydrated and anhydrobiotic adults. In contrast, anhydrobiotic eggs (50% lethal dose; 1690 Gy) were substantially more radioresistant than hydrated ones (50% lethal dose; 509 Gy). Anhydrobiotic eggs also have a broader temperature resistance compared with hydrated ones. Over 70% of the anhydrobiotic eggs treated at either -196°C or +50°C hatched successfully, but all the hydrated eggs failed to hatch. After exposure to high-vacuum conditions (5.3×10(-4) Pa to 6.2×10(-5) Pa), the hatchability of the anhydrobiotic eggs was comparable to that of untreated control eggs.     

Analysis of the pre-flight and post-flight calibration procedures performed on the Liulin space radiation dosimeter

Liulin, a dosimetry-radiometry system, was developed to satisfy the requirements for active flux and dose rate measurements for the flight of the second Bulgarian cosmonaut in 1988. The system consists of a compact battery-operated silicon solid state detector unit and a read/write microcomputer and telemetry unit. We describe the pre-flight calibrations with charged particles, using radioactive sources and accelerated 170 MeV/nucleon proton and alpha particles at the Dubna, Russia cyclotron. We discuss comparisons with data obtained on Mir with the French-built tissue equivalent LET spectrometer NAUSICAA. Lastly, we describe post-flight calibrations performed with 1 GeV/nucleon 56Fe ions at the Brookhaven National Laboratory AGS accelerator, where the instrument was mounted in tandem with several thin position-sensitive silicon detectors behind a stopping target. The silicon detectors provided an energy spectrum for the surviving charged nuclear fragments for which the flux and absorbed dose were recorded by Liulin.     

Cosmic radiation exposure of biological test systems during the EXPOSE-E mission

In the frame of the EXPOSE-E mission on the Columbus external payload facility EuTEF on board the International Space Station, passive thermoluminescence dosimeters were applied to measure the radiation exposure of biological samples. The detectors were located either as stacks next to biological specimens to determine the depth dose distribution or beneath the sample carriers to determine the dose levels for maximum shielding. The maximum mission dose measured in the upper layer of the depth dose part of the experiment amounted to 238±10 mGy, which relates to an average dose rate of 408±16 μGy/d. In these stacks of about 8?mm height, the dose decreased by 5-12% with depth. The maximum dose measured beneath the sample carriers was 215±16 mGy, which amounts to an average dose rate of 368±27 μGy/d. These values are close to those assessed for the interior of the Columbus module and demonstrate the high shielding of the biological experiments within the EXPOSE-E facility. Besides the shielding by the EXPOSE-E hardware itself, additional shielding was experienced by the external structures adjacent to EXPOSE-E, such as EuTEF and Columbus. This led to a dose gradient over the entire exposure area, from 215±16 mGy for the lowest to 121±6 mGy for maximum shielding. Hence, the doses perceived by the biological samples inside EXPOSE-E varied by 70% (from lowest to highest dose). As a consequence of the high shielding, the biological samples were predominantly exposed to galactic cosmic heavy ions, while electrons and a significant fraction of protons of the radiation belts and solar wind did not reach the samples.     

Recent results of the joint ESA-DARA/IBMP experiments Biokosmos "Seeds"

Comparison of experimental data obtained from short (SDEF) and long duration exposure flights (LDEF) have recently led to results which will be significant for longer and/or repeated sojourn of man in space. Under orbital conditions biological stress and damage are induced in test subjects by cosmic radiation, especially the high energetic, densely ionizing component of heavy ions. Plant seeds were successful model systems for a biotest in studying the physiological damages and mutagenic effect caused by ionizing cosmic radiation in particular stem cells. Dosimetrically, the subdivision into charge- and Let-groups reveals the contribution of the intermediate group (LET = 350-1000 MeV/cm) due to the medium heavy ions (Z = 6-10). Their relative contribution increases with the lower inclination of the orbit of LDEF-1; on the other hand, the total fluence becomes higher with longer duration of the flight. The observed endpoints of the biological radiation damage hint at a correlation with particle dose rate rather than with the dose; additionally, data on shielding effects inside and outside the space craft and its exposure were gained from the different SDEF- and LDEF-missions.     

Ground-based simulations of cosmic ray heavy ion interactions in spacecraft and planetary habitat shielding materials

This paper surveys some recent accelerator-based measurements of the nuclear fragmentation of high energy nuclei in shielding and tissue-equivalent materials. These data are needed to make accurate predictions of the radiation field produced at depth in spacecraft and planetary habitat shielding materials and in the human body by heavy charged particles in the galactic cosmic radiation. Projectile-target combinations include 1 GeV/nucleon 56Fe incident on aluminum and graphite and 600 MeV/nucleon 56Fe and 290 MeV/nucleon 12C on polyethylene. We present examples of the dependence of fragmentation on material type and thickness, of a comparison between data and a fragmentation model, and of multiple fragments produced along the beam axis.     

Time Variations of Low-Energy Protons and Helium Nuclei (0.3–10 MeV/nucleon) during the Quiescent Time of Solar Activity in the Inner Heliosphere

Time variations with a duration of 0.6–1.5 years are studied in the interplanetary space for protons and helium nuclei with energies of 0.3–10 MeV per nucleon at a quiescent time of solar activity. It is shown that at 1 AU in the periods 1978–1981 and 1988–1990, at the phases of growth of the 21st and 22nd solar cycles, the background fluxes of these particles determined as minimum intensity levels in every month increased demonstrating steplike variations. At the same time, the intensity of galactic cosmic rays (GCRs) decreased also with the formation of modulation steps. Each step of low-energy particles was finished by a deep minimum of intensity (gap) in both protons and helium nuclei and with a simultaneous short-term increase of the GCR intensity. We present the results of studying five such steps in the intensity of low-energy particles that were observed simultaneously and were opposite in phase with modulation steps of galactic particles. The lowest values of the H/He ratio were recorded at the end of every step, at the lowest intensities of these particles, i.e., in the gap. The true background population at 1 AU was detected precisely at these time intervals, when the contributions of flare particles and those accelerated in the international space were minimum. Various possibilities of the origination of the steplike variations of the background fluxes of protons and helium nuclei with energies of 0.3–10 MeV per nucleon, correlated with similar GCR variations, are discussed.     

SiCp/Al复合材料的Fe11+离子辐照实验研究

为了解辐照对航天电子元器件封装基板复合材料SiC_p/Al的影响,利用3 MeV Fe11+束流进行了不同注量下的辐照实验。辐照完成后,利用X射线衍射（XRD）仪和纳米压痕仪对材料进行表征分析。XRD分析表明,Fe11+离子入射后材料辐照层应力发生了变化。结合对应的理论模型,对铝基体的位错密度进行计算,发现辐照后铝基体的位错密度增加,SiC颗粒产生非晶化。纳米压痕测试表明,随着离子注量增加,材料辐照硬化程度增加。综上,辐照硬化可能是由于铝合金基体的应力变化导致产生局部高密度位错区,而这些区域阻碍了后续位错的运动,进而导致位错聚集,最终使得材料硬度增加。     

原子氧对航天材料的影响与防护

采用质子加速器对Fe-Ni软磁合金进行8 MeV质子辐照,研究其磁性能及组织结构与缺陷变化情况。研究结果表明,随质子辐照吸收剂量的增加,Fe-Ni合金的矫顽力增大,磁导率下降,饱和磁感应强度未受影响。X射线衍射（XRD）结果表明辐照未引起相结构的变化,但使晶格发生了畸变。正电子湮没分析表明,辐照导致了空位等材料缺陷。     

Evaluation of dose rate reduction in a spacecraft compartment due to additional water shield

The dose reduction rates brought about by the installation of additional water shielding in a spacecraft are calculated in the paper using the particles and heavy ion transport code system PHITS, which can deal with transport of all kinds of hadrons and heavy ions with energies up to 100 GeV/n in three-dimensional phase spaces. In the PHITS simulation, an imaginary spacecraft was irradiated isotropically by cosmic rays with charges up to 28 and energies up to 100 GeV/n, and the dose reduction rates due to water shielding were evaluated for 5 types of doses: the dose equivalents obtained from the LET and linear energy spectra, the dose equivalents to skin and red bone marrow, and the effective dose equivalent. The results of the simulation indicate that the dose reduction rates differ according to the type of dose evaluated. For example, 5 g/cm² water shielding reduces the effective dose equivalent and the LET dose equivalent by approximately 14% and 32%, respectively. Such degrees of dose reduction can be regarded to make water shielding worth the efforts required to install it.     

大容量Flash存储器空间辐射效应试验研究

分析了商用Flash存储器应用于航天器时应考虑的空间辐射效应和机理,并利用钴-60γ射线和重离子加速器对韩国三星公司生产的大容量Flash存储器K9XXG08UXA系列进行了抗电离总剂量试验和抗单粒子试验,以评估其空间应用可行性。试验结果显示：这一系列存储器的累积电离总剂量为50krad（Si）时,器件部分数据丢失,重...     

Establishment of a rearing system of the extremotolerant tardigrade Ramazzottius varieornatus: a new model animal for astrobiology

Studies on the ability of multicellular organisms to tolerate specific environmental extremes are relatively rare compared to those of unicellular microorganisms in extreme environments. Tardigrades are extremotolerant animals that can enter an ametabolic dry state called anhydrobiosis and have high tolerance to a variety of extreme environmental conditions, particularly while in anhydrobiosis. Although tardigrades have been expected to be a potential model animal for astrobiological studies due to their excellent anhydrobiotic and extremotolerant abilities, few studies of tolerance with cultured tardigrades have been reported, possibly due to the absence of a model species that can be easily maintained under rearing conditions. We report the successful rearing of the herbivorous tardigrade, Ramazzottius varieornatus, by supplying the green alga Chlorella vulgaris as food. The life span was 35 +/- 16.4 d, deposited eggs required 5.7 +/- 1.1 d to hatch, and animals began to deposit eggs 9 d after hatching. The reared individuals of this species had an anhydrobiotic capacity throughout their life cycle in egg, juvenile, and adult stages. Furthermore, the reared adults in an anhydrobiotic state were tolerant of temperatures of 90 degrees C and -196 degrees C, and exposure to 99.8% acetonitrile or irradiation with 4000 Gy (4)He ions. Based on their life history traits and tolerance to extreme stresses, R. varieornatus may be a suitable model for astrobiological studies of multicellular organisms.     

The neutron,gamma-ray,X-ray spectrometer (NGXS): A compact instrument for making combined measurements of neutrons,gamma-rays,and X-rays

The Neutron, Gamma ray, and X-ray Spectrometer (NGXS) is a compact instrument designed to detect neutrons, gamma-rays, and hard X-rays. The original goal of NGXS was to detect and characterize neutrons, gamma-rays, and X-rays from the Sun as part of the Solar Probe Plus mission in order to provide direct insight into particle acceleration, magnetic reconnection, and cross-field transport processes that take place near the Sun. Based on high-energy neutron detections from prompt solar flares, it is estimated that the NGXS would detect neutrons from 15 to 24 impulsive flares. The NGXS sensitivity to 2.2 MeV gamma rays would enable a detection of ∼50–60 impulsive flares. The NGXS is estimated to measure ∼120 counts/s for a GOES C1-type flare at 0.1 AU, which allows for a large dynamic range to detect both small and large flares.     

Neutral buoyancy and sleep-deprived serum factors alter expression of cytokines regulating osteogenesis

We examined expression of genes associated with cytokine production, and genes implicated in regulating bone metabolism, in bone stromal and osteoblast cells incubated under standard ground conditions and under conditions of neutral buoyancy, and in the presence/absence of serum from normal or sleep-deprived mice. We observed a clear interaction between these two conditions (exposure to neutral buoyancy and serum stimulation) in promoting enhanced osteoclastogenesis. Both conditions independently altered expression of a number of cytokines implicated in the regulation of bone metabolism. However, using stromal cells from IL-1 and TNFx cytokine(r) KO mice, we concluded that the increased bone loss under microgravity conditions was not primarily cytokine mediated.     

Probabilistic assessment of radiation risk for astronauts in space missions

Accurate estimations of the health risks to astronauts due to space radiation exposure are necessary for future lunar and Mars missions. Space radiation consists of solar particle events (SPEs), comprised largely of medium energy protons (less than several hundred MeV); and galactic cosmic rays (GCR), which include high-energy protons and heavy ions. While the frequency distribution of SPEs depends strongly upon the phase within the solar activity cycle, the individual SPE occurrences themselves are random in nature. A solar modulation model has been developed for the temporal characterization of the GCR environment, which is represented by the deceleration potential, ?. The risk of radiation exposure to astronauts as well as to hardware from SPEs during extra-vehicular activities (EVAs) or in lightly shielded vehicles is a major concern for radiation protection. To support the probabilistic risk assessment for EVAs, which could be up to 15% of crew time² on lunar missions, we estimated the probability of SPE occurrence as a function of solar cycle phase using a non-homogeneous Poisson model [1] to fit the historical database of measurements of protons with energy>30 MeV, Φ₃₀. The resultant organ doses and dose equivalents, as well as effective whole body doses, for acute and cancer risk estimations are analyzed for a conceptual habitat module and for a lunar rover during space missions of defined durations. This probabilistic approach to radiation risk assessment from SPE and GCR is in support of mission design and operational planning for future manned space exploration missions.     

Detection of DNA damages and repair in human culture cells with simulated space radiation

DNA damages and its repair of cultured WI38 human fibroblast cells and T98G human glioblastoma cells were studied by exposing to carbon ion beams of HIMAC accelerator. The exposed cells were incubated at 37 °C for appropriate intervals and the damages were analyzed by alkaline comet assay and quantitative RT-PCR with p53 mRNA Highly inhomogeneous DNA damages were observed among the electrophoretic cell images of the comet assay. The degree of the damages was analyzed semi-quantitatively by using the Comet Index. The damaged fraction of WI38 cells was 85% immediately after 4 Gy (100 keV/μm) irradiation and decreased to 50% after 120 min. incubation indicating a repair of cell DNA. Time dependent p53 gene expression was also analyzed by the quantitative RT-PCR method.     

用10 MeV质子和钴60 γ射线进行CCD空间辐射效应评估

文章用10MeV质子和钴60γ射线对CCD(Charge Coupled Device)进行了辐照试验,分别计算得到了电离总剂量和位移效应的失效剂量。通过分析比较得出:在空间环境中,相对于电离总剂量效应而言,位移效应对CCD的损伤更为严重。因此,进行CCD辐射效应评估时,不仅要考虑电离总剂量效应,还要考虑位移效应。文章还探讨了评估CCD抗位移损伤能力的方法。     

高加速近紫外模拟的均匀性控制方法

在对航天器敏感材料开展高加速紫外模拟试验的过程中,需要对近紫外辐照模拟的均匀性进行控制,以提高试验模拟的有效性和试验结果的准确性。文章采用氙灯作为紫外辐照源,利用聚光镜、光学积分器和反射/滤光镜,实现了大面积高均匀度的紫外辐照;同时,利用准直镜和发散镜的设计,成功兼顾高加速倍率和较大辐照面积,可实现?300 mm面积上紫外辐照度为15SC、?180 mm面积上紫外辐照度为24SC、均匀性均优于95%的紫外模拟。     

γ射线辐射对空间光学玻璃透射率的影响

利用60Co γ-射线对钡冕玻璃（BaK3）、镧冕玻璃（LaK3）、火石玻璃（F10）、镧火石玻璃（LaF3）、轻火石玻璃（QF3）和重火石玻璃（ZF4）进行辐照,研究不同辐照剂量对光学透射率的影响及这些玻璃在空间光学系统中的适应性。光学透射率测试范围为400~1 100 nm。结果表明所有玻璃在辐照后可见光透射率都下降了,而在近红外波段下降不明显（除了QF3和LaF3）。尽管F10和QF3光学透射率在辐照前相似,但是辐照后F10衰减是所有玻璃中最小的,而QF3衰减最为严重。研究发现,当达到一定辐照剂量后,玻璃材料的透射率不再继续衰减,而是趋于稳定。这些结果为空间光学系统针对辐射进行冗余设计提供了依据。