UV photobiochemistry of anhydrobiotic organisms at extremely low temperatures

Spores of Bacillus subtilis (TKJ 3412), cells of Deinococcus radiodurans R₁ (wild type) and conidia of Aspergillus ochraceus (strain 3174) have been UV irradiated (254 nm) in the dry state (3% relative humidity, argon) or in aqueous suspension at room temperature, at −55°C to −70°C and at −165°C to −170°C. The following effects have been analyzed: decrease in viability, occurrence of DNA strand breaks (pulsed-field gel electrophoresis) and production of DNA-protein cross-links (membrane filter method). The loss in viability is usually more pronounced at around −70°C than at room temperature, but it is lowest around −170°C. The kind of prevailing DNA damage varies from organism to organism. The amount of UV induced DNA-protein cross-link products steadily decreases with the temperature and is lowest at −170°C. The decrease in highly polymeric DNA by double strand breaks follows no universal pattern. The observed hypersensitivity of the three very different species at −70°C can therefore not be simply explained on the basis of the number of DNA lesions analyzed in the course of this work. We suggest that also the changing state of cellular water below and above about −130°C significantly contributes to the change in photosensitivity.     

Microbial processes in frozen food

Deep freezing of food and storage at −19°C is a standard conservation procedure in food technology. The lower limit of growth of bacteria in food is from about −5°C to about −8°C, whereas the reproduction limit of yeasts is 2 to 3°C lower. Storage temperatures above −10°C should therefore not be used. At −18°C, a commonly used storage temperature, no growth of microorganisms will occur. The microorganisms mainly found at the lower growth limit are Pseudomonas sp. and basidiomycete yeasts. The reduction in the number of microorganisms due to freezing, storage, and thawing is not of practical importance. Microbial enzymes, in particular lipases and proteases, are still active at −18°C. Therefore, the quality of raw products and good hygiene at the production site are most important.     

Cold resistance and metabolic activity of lichens below 0°C

Laboratory measurements show that lichens are extremely tolerant of freezing stress and of low-temperature exposure. Metabolic activity recovered quickly after severe and extended cold treatment. Experimental results demonstrate also that CO₂ exchange is already active at around −20°C. The psychrophilic character of polar lichen species is demonstrated by optimum temperatures for net photosynthesis between 0 and 15°C. In situ measurements show that lichens begin photosynthesizing below 0°C if the dry thalli receive fresh snow. The lowest temperature measured in active lichens was −17°C at a continental Antarctic site. The fine structure and the hydration state of photobiont and mycobiont cells were studied by low-temperature scanning electron microscopy (LTSEM) of frozen hydrated specimens. Water potentials of the frozen system are in the range of or even higher than those allowing dry lichens to start photosynthesis by water vapor uptake at +10°C. The great success of lichens in polar and high alpine regions gives evidence of their physiological adaptation to low temperatures. In general lichens are able to persist through glacial periods, but extended snow cover and glaciation are limiting factors.     

Geotropic sensitivity exhibited by single hornets: The influence of caste, age, light and temperature

Hornet ( , Hymenoptera: Vespinae) workers, queens and males, aged 0–24 hours (i.e. juveniles) and 24 hours and more (i.e. adults) were tested for their responses to changes in the direction of the gravitational force while placed on a flat surface gradually tilted between 0.5° and 180°. The tests were run on non-blind and blind hornets, at temperatures ranging between 18°C and 35°C, in daylight as well as in the dark. Up to 18 hours of age, negative phototaxis prevailed among the hornets, which displayed a clear preference for remaining in the dark regardless of the geotropic position. Between 18–24 hours of age, there was gradual appearance of a sensitivity to change in the geotropic position. Above 24 hr of age, the hornets became sensitive to changes in their declinations, with workers becoming sensitive at a 3–5° declination, queens at 4–5° and males at a declination of 8–19° from the horizontal. Hornet response takes the form of an upward climb, to the highest point of the test surface. Such response required a temperature exceeding 24.8–25°C for workers, 23.2°C for queens and 20.8–21°C for males.     

Geminga pulsar observations with gamma-telescope Gamma-1

The Geminga light curve obtained with the “Gamma-1” telescope features two peaks separated by 0.5 ± 0.03 period. The light curve is pronounced for γ-quanta energies higher than 400 MeV. The pulsed flux upper limit (1σ) in the energy interval 50 – 300 MeV is 6·10⁻⁷ cm⁻²sec⁻¹. For energies >300 MeV the pulsed component power law spectrum has an exponent 1.1 _−0.3^+1.1 and an integral flux (1.1±0.3)·10⁻⁶ cm⁻²sec⁻¹.     

CMB anisotropy testing from small satellites

We review the advantages and possibilities of small satellites. New results of data reduction of the satellite-borne experiment RELICT-1 are presented. For the inflation spectrum of primordial perturbations we obtained the estimate for quadrupole component 6·10⁻⁶ <ΔT₂/T<3.3·10⁻⁶. The RELICT-2 mission will provide a possibility of measurement of CBR anisotropy down to the level less than ΔT₂/T = 10⁻⁸. We present the results of engineering testing of RELICT-2 measurement system and discuss ways of improving of the radiometers sensitivities.     

High temperature alters the growth reaction of Pottia protonemata.

Under gravistimulation, dark-grown protonemata of Pottia intermedia revealed negative gravitropism with a growth rate of approximately 28 μm·h⁻¹ at room temperature (20 °C). In 7 days, the protonema formed a bundle of vertically oriented filaments. At an elevated temperature (30 °C), bundles of vertically growing filaments were also formed. However, both filament growth rate and amplitude of the gravicurvature were reduced. Red light (RL) irradiation induced a positive phototropism of most apical protonemal cells at 20 °C. In a following period of darkness, approximately two-thirds of such cells began to grow upward again, recovering their negative gravitropism. RL irradiation at the elevated temperature caused a partial increase in the number of protonemal cells with negative phototropism, but the protonemata did not exhibit negative gravitropism after transfer to darkness. The negative gravitropic reaction was renewed only when protonemata were placed at 20 °C. A dramatic decrease in starch amount in protonemal apical cells, which are sensitive to both gravity and light, occurred at the higher temperature. Such a decrease may be one of the reasons for the inhibition of the protonemal gravireaction at the higher temperature. The observation has a bearing on the starch-statolith theory.     

Towards validation of SCIAMACHY lunar occultation NO2 vertical profiles

Vertical profiles of stratospheric nitrogen dioxide (NO₂) have been retrieved from moderate resolution lunar occultation transmission spectra measured by Scanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY) on board the European Environmental Satellite (ENVISAT). These measurements were taken over the high southern latitude of 50°–90° during the period of 2003–2005. To assess the accuracy of the retrieved NO₂ profiles, the SCIAMACHY nighttime NO₂ profiles were compared with NO₂ profiles retrieved from sunrise solar occultation spectra measured by the Halogen Occultation Experiment (HALOE) and the Stratospheric Aerosol and Gas Experiments II (SAGE II) using a photochemical correction model. The validation results show good agreement of SCIAMACHY lunar occultation NO₂ with scaled HALOE and SAGE II profiles. The relative mean differences (rmd) with scaled HALOE profiles are within −13% to +5% and standard deviations (rms) of the relative differences are within 3–19% between 25 and 38 km. The rmd and rms with scaled SAGE II NO₂ profiles are in the range of −9 to +7 and 10–17% respectively between 22 and 39 km.     

Near infrared observations of comet Halley from Vega 2

Spatial distribution of the continuum radiation in the range of 0.95–1.9 μm presumes total dust production rate of the comet of 10ρ tonne s⁻¹ (ρ is the dust material density) and its angular distribution proportional cos . Observations of the water vapor band at 1.38 μ m reveal strong jets, their time shift from the dust jet measured in situ is consistent with gas velocity of 0.82±0.1 km s⁻¹ and dust velocity of 0.55±0.08 km s⁻¹. The OH vibrational-rotational bands observed are excided directly via photolysis of water vapor. Water vapor production rate deduced from the H₂O band and OH band intensities is 8×10²⁹ s⁻¹. Intensity of the CN(0,0) band result in the CN column density of 9×10¹² cm⁻², i.e. larger by a factor of 3 than given by the violet band.     

Mercury: can any ice exist at subpolar regions?

The heat transfer in a regolith subsurface layer of thickness 20 m has been theoretically simulated for the areas near Mercury's north pole aiming at the clarification of the possible existence of subsurface ice formations of different form. The paper considers different models of the icy regolith structure and composition: pure uniform amorphous ice; a porous dispersive system with ice-filled pores and voids; permafrost. For comparison the heat transfer in dry iceless regolith has been considered as well. It has been shown that the line of maximum distribution of subsurface icy formations depends on the icy regolith model, but for any one in the “hot” regions it does not go below 70°. For the “cool” regions this line has been shown to go from 5° to 10° southward than that for the “hot” ones. The possible thickness of icy regolith near the pole has been estimated for different models assuming an interior heat flow of 15 mW m⁻². It has been shown that the maximum thickness of this layer takes place at the pole and is equal to 10 km for any model.     

Observations of diffuse galactic positron annihilation radiation

The Galactic plane was scanned nearly three times by the UCSD/MIT Hard X-Ray and Low Energy Gamma-Ray Experiment on HEAO-1 from August 1977 through September 1978. Its Medium Energy Detectors were of the NaI/CsI phoswich type and operated over the 100 keV to 2 MeV range, with a 17° FWHM field of view and a 9% energy resolution at 511 keV. Sky maps for each epoch of observation were constructed in several energy bands. After subtraction of known point sources, a component associated with the galactic plane remains, whose spectrum is consistent with a power law and a positron annihilation spectrum. In the 333 to 583 keV energy band the flux is concentrated within ±35° of the galactic center, and the ratio of flux/radian (anticenter) is high, with a 2σ lower limit of 13. The parameters of the galactic center region's annihilation spectrum are positronium fraction of 0.9±0.1 and 511 keV flux of (2.0±0.7)×10⁻³ photons/cm²-sec-rad.     

High spectral resolution Fabry-Perot interferometer measurements of comet Halley at H-alpha and 6300 Å

A 40.6 cm Newtonian telescope has been interfaced to the Fabry-Perot interferometer at the Arecibo Observatory to make high spectral resolution measurements of Comet Halley emissions at 6562.72 Å (H-alpha) and 6300.3 Å (OI). In March 1986 the H-alpha surface brightness for a 5′.9 field of view centered on the comet nucleus decreased from 39±7.8 rayleighs on 12 March to 16±3.8 rayleighs on 23 March. The atomic hydrogen production rate on 12 March 1986 was 1.62±0.5 × 10³⁰ s⁻¹, and on 23 March 1986 it was 6.76±2.3 × 10²⁹ s⁻¹. Using spectral resolution of 0.196 Å, we found the atomic hydrogen outflow velocity to be approximately 7.9±1.0 km s⁻¹. In general, the H-alpha spectra are highly structured, and indicative of a multiple component atomic hydrogen velocity distribution. An isotropic outflow of atomic hydrogen at various velocities is not adequate to explain the spectra measured at H-alpha. The 6300.3 Å emission of O(1D) had a surface brightness of 81±16 rayleighs on 15 March 1986, and 95±11 rayleighs on 17 March 1986. After adjustment for atmospheric extinction, the implied O(1D) production rate on 15 March is 6.44±3.0 × 10²⁸ s⁻¹, and the production rate on 17 March is 5.66±2.7 × 10²⁸ s⁻¹. These spectra included a feature at 6300.8 Å that we attribute to NH₂. The brightness of this emission feature was 37±11 rayleighs on 15 March.     

Properties of narrow coronal massejections observed with LASCO

We report the statistical properties of narrow coronal mass ejections (CMEs, angular width < 20°) withparticular emphasis on comparison with normal CMEs. We investigated 806 narrow CMEs from an online LASCO/CME catalog and found that (1) the fraction of narrow CMEs increases from 12% to 22% towards solar maximum, (2) during the solar maximum, the narrow CMEs are generally faster than normal ones, (3) the maximum speed of narrow CMEs (1141 km s⁻¹) is much smaller than that of the normal CMEs (2604 km s⁻¹). These results imply that narrow CMEs do not form a subset of normal CMEs and have a different acceleration mechanism from normal CMEs.     

Neutral upper atmospheres of Venus and Mars

Numerous measurements of the neutral upper atmosphere above 100 km have been made from spacecraft over Venus and over Mars. The Venus exospheric temperatures are unexpectedly low (less than 300°K near noon and less than 130°K near midnight). These very low temperatures may be partially caused by collisional excitation of CO₂ vibrational states by atomic oxygen and partially by eddy cooling. The Venus atmosphere is unexpectedly insensitive to solar EUV variability. On the other hand, the Martian dayside exospheric temperature varies from 150°K to 400°K over the 11-year solar cycle, where CO₂ 15-μm cooling may be less effective because of lower atomic oxygen mixing ratios. On Venus, temperature increases with altitude on the dayside (thermosphere), but decreases with altitude from 100 to 150 km on the nightside (cryosphere). However, dayside Martian temperatures near solar minimum for maximum planet-sun distance and low solar activity are essentially isothermal from 40 km to 200 km. During high solar activity, the thermospheric temperatures of Mars sharply increase. The Venus neutral upper atmosphere contains CO₂, O, CO, C, N₂, N, He, H, D and hot nonthermal H, O, C, and N, while the dayside Mars neutral upper atmosphere contains CO₂, O, O₂, CO, C, N₂, He, H, and Ar. There is evidence on Venus for inhibited day-to-night transport as well as superrotation of the upper atmosphere. Both atmospheres have substantial wave activity. Various theoretical models used to interpret the planetary atmospheric data are discussed.     

Large light X-ray optics: basic ideas and concepts

One of the main guidelines for future X-ray astronomy projects like, e.g., XEUS (ESA) and Generation-X (NASA) is to utilize grazing-incidence focusing optics with extremely large telescopes (several tens of m² at 1 keV), with a dramatic increase in collecting area of about two order of magnitude compared to the current X-ray telescopes. In order to avoid the problem of the source's confusion limit at low fluxes, the angular resolution required for these optics should be superb (a few arcsec at most). The enormous mirror dimensions together with the high imaging performances give rise to a number of manufacturing problems. It is basically impossible to realize so large mirrors from closed Wolter I shells which benefit from high mechanical stiffness. Instead the mirrors need to be formed as rectangular segments and a series of them will be assembled in a petal. Taking into account the realistic load capabilities of space launchers, to be able to put in orbit so large mirror modules the mass/geometric-area ratio of the optics should be very small. Finally, with a so large optics mass it would be very difficult to provide the electric power for an optics thermal active control, able to maintain the mirrors at the usual temperature of 20 °C. Therefore, very likely, the optics will instead operate in extreme thermal conditions, with the mirror temperature oscillating between −30 and −40 °C, that tends to exclude the epoxy replication approach (the mismatch between the CTE of the substrate and that of the resin would cause prohibitively large deformations of the mirror surface profiles). From these considerations light weight materials with high thermal–mechanical properties such as glass or ceramics become attractive to realize the mirrors of future Xray telescopes. In this paper, we will discuss a segments manufacturing method based on Borofloat^TM glass. A series of finite element analysis concerning different aspects of the production, testing and integration of the optics are also presented as well.     

Interplanetary scintillations of PKS 2314+03 during occultation by comet Halley

Strong interplanetary scintillations (IPS) of the quasar 2314+03 were recorded at 103 MHz at Thaltej-Ahmedabad, India with a transit type correlation interferometer on 18, 19 and 20 December 1985, as the radio source was predicted to be occulted by the ion tail of the comet Halley.

On 18th through 20th very strong scintillations, with periodicities of 1 sec average were observed, their amplitude progressively decreasing as the source approached the tail-end. The rms variations of scintillating flux of the source on 18, 19 & 20 were about 18, 11 & 4.7 Jy, as against 3.3 Jy on control days 17 and 21 December for solar elongation of 87°.

Assuming Gaussian irregularities with weak scattering, the rms density variations, ΔN, of 10, 6, 3 and 1 elec./cm³ on 18 through 21 December, from the comet nucleus towards its tail-end, varied as (ΔN) ∝ r^−3.3 as against (ΔN) ∝ r⁻² in the solar plasma.

Quasi-periodic modulations of the enhanced scintillating flux possibly imply 10⁴ km scale-size ion condensations and mean electron density of 10³ to 10⁴ electrons/cm³ in the Halley's plasma tail.     

Mesospheric nitric oxide and ozone measurements in polar winter at 69°N

Two rocket experiments were carried out just before and after the polar night at Andoya (69°N), Norway to investigate transport of nitric oxide produced by auroral processes into the middle atmosphere and its influence on the ozone chemistry. Nitric oxide densities of (2–5) × 10⁸cm⁻³ found in the 70–90 km region are one to two orders of magnitude larger than those at middle latitudes. The influence on ozone densities in the 70–90 km region due to such enhanced nitric oxide abundance is found to be insignificant as compared to that due to transport in the middle of February. The larger ozone densities found in February (in spite of longer sunlit duration) than in November in the 40–60 km region again support predominance of transport over photochemical loss.     

Calcium abundance measurements using the Yohkoh BCS

Soft X-ray observations by SMM and other spacecraft have shown that the abundance of certain elements in solar corona varies from flare to flare. In this study, observations made by the Yohkoh Bragg Crystal Spectrometer (BCS) in helium-like Ca XIX have been analysed, and Ca abundance determined for 177 flares observed during the first four years of the mission (1991–1995). The average abundance of Ca relative to H for all flares is A_Ca = (3.64±0.39) × 10⁻⁶. As with an earlier study of SMM data, the abundance is found to be enhanced compared to the photosphere ((2.24±0.10) × 10⁻⁶), and with only minor variation from flare to flare. However, the absolute value and range of values determined by this study is smaller than in the previous study; these differences are discussed.     

A study of the solar cycle signal on total ozone over low-latitude Brazilian observation stations

Observations of total ozone at low latitudes in Brazil have been made using Dobson spectrophotometers since 1974 for Cachoeira Paulista (23.1° S, 45° W) and since 1978 for Natal (5.8° S, 35.2° W). Annual averages, 12 months and 36 months running averages have been analyzed. Spectral analyses of the data revealed that the most important periods found (confidence level> 90%) were: for Natal, 2.5 years (93.1%, quasi-biennial oscillation-QBO) and 10 years (98,2%, possibly the solar cycle signal); for Cachoeira Paulista, 2.4 years (96.8%, QBO) and 8 years (99.6%). The difference in total ozone between maximum and minimum solar cycles were estimated, using yearly averages of total ozone. For solar cycle 21, 1.16% and 1.26% for Natal and Cachoeira Paulista were found; for solar cycle 22, a larger difference of 3.8% for Natal and 4.1% for Cachoeira Paulista were found. The corresponding variation in UV-B at 300 nm, using Beer's law, is 8–10% for C. Paulista and 4–5% for Natal, with maxima occurring during the minimum of the solar cycle.