Cave biosignature suites: microbes,minerals, and Mars

Earth's subsurface offers one of the best possible sites to search for microbial life and the characteristic lithologies that life leaves behind. The subterrain may be equally valuable for astrobiology. Where surface conditions are particularly hostile, like on Mars, the subsurface may offer the only habitat for extant lifeforms and access to recognizable biosignatures. We have identified numerous unequivocally biogenic macroscopic, microscopic, and chemical/geochemical cave biosignatures. However, to be especially useful for astrobiology, we are looking for suites of characteristics. Ideally, "biosignature suites" should be both macroscopically and microscopically detectable, independently verifiable by nonmorphological means, and as independent as possible of specific details of life chemistries--demanding (and sometimes conflicting) criteria. Working in fragile, legally protected environments, we developed noninvasive and minimal impact techniques for life and biosignature detection/characterization analogous to Planetary Protection Protocols. Our difficult field conditions have shared limitations common to extraterrestrial robotic and human missions. Thus, the cave/subsurface astrobiology model addresses the most important goals from both scientific and operational points of view. We present details of cave biosignature suites involving manganese and iron oxides, calcite, and sulfur minerals. Suites include morphological fossils, mineral-coated filaments, living microbial mats and preserved biofabrics, 13C and 34S values consistent with microbial metabolism, genetic data, unusual elemental abundances and ratios, and crystallographic mineral forms.     

Desperately seeking life on Mars

Humans appear to be obsessed with the idea of finding life on Mars and have latched on to any evidence—however improbable—that might support its existence. Charting the history of the often deceptive scientific (not to mention literary) findings made about Mars, this viewpoint suggests that our desire to find extraterrestrial life says more about the human need for companionship and communication than about the true past of the planet. Nevertheless, ESA's Mars Express should give us a better understanding of the true likelihood of life ever having existed there after its launch in 2003.     

Gypsum-permineralized microfossils and their relevance to the search for life on Mars

Abstract Orbital and in situ analyses establish that aerially extensive deposits of evaporitic sulfates, including gypsum, are present on the surface of Mars. Although comparable gypsiferous sediments on Earth have been largely ignored by paleontologists, we here report the finding of diverse fossil microscopic organisms permineralized in bottom-nucleated gypsums of seven deposits: two from the Permian (～260?Ma) of New Mexico, USA; one from the Miocene (～6?Ma) of Italy; and four from Recent lacustrine and saltern deposits of Australia, Mexico, and Peru. In addition to presenting the first report of the widespread occurrence of microscopic fossils in bottom-nucleated primary gypsum, we show the striking morphological similarity of the majority of the benthic filamentous fossils of these units to the microorganisms of a modern sulfuretum biocoenose. Based on such similarity, in morphology as well as habitat, these findings suggest that anaerobic sulfur-metabolizing microbial assemblages have changed relatively little over hundreds of millions of years. Their discovery as fossilized components of the seven gypsiferous units reported suggests that primary bottom-nucleated gypsum represents a promising target in the search for evidence of past life on Mars. Key Words: Confocal laser scanning microscopy-Gypsum fossils-Mars sample return missions-Raman spectroscopy-Sample Analysis at Mars (SAM) instrument-Sulfuretum. Astrobiology 12, 619-633.     

Fluid inclusion studies of chemosynthetic carbonates: strategy for seeking life on Mars

Fluid inclusions in minerals hold the potential to provide important data on the chemistry of the ambient fluids during mineral precipitation. Especially interesting to astrobiologists are inclusions in low-temperature minerals that may have been precipitated in the presence of microorganisms. We demonstrate that it is possible to obtain data from inclusions in chemosynthetic carbonates that precipitated by the oxidation of organic carbon around methane-bearing seepages. Chemosynthetic carbonates have been identified as a target rock for astrobiological exploration. Other surficial rock types identified as targets for astrobiological exploration include hydrothermal deposits, speleothems, stromatolites, tufas, and evaporites, each of which can contain fluid inclusions. Fracture systems below impact craters would also contain precipitates of minerals with fluid inclusions. As fluid inclusions are sealed microchambers, they preserve fluids in regions where water is now absent, such as regions of the martian surface. Although most inclusions are < 5 microns, the possibility to obtain data from the fluids, including biosignatures and physical remains of life, underscores the advantages of technological advances in the study of fluid inclusions. The crushing of bulk samples could release inclusion waters for analysis, which could be undertaken in situ on Mars.     

The potential for lithoautotrophic life on Mars: application to shallow interfacial water environments

We developed a numerical model to assess the lithoautotrophic habitability of Mars based on metabolic energy, nutrients, water availability, and temperature. Available metabolic energy and nutrient sources were based on a laboratory-produced Mars-analog inorganic chemistry. For this specific reference chemistry, the most efficient lithoautotrophic microorganisms would use Fe(2+) as a primary metabolic electron donor and NO(3)(-) or gaseous O(2) as a terminal electron acceptor. In a closed model system, biomass production was limited by the electron donor Fe(2+) and metabolically required P, and typically amounted to approximately 800 pg of dry biomass/ml ( approximately 8,500 cells/ml). Continued growth requires propagation of microbes to new fecund environments, delivery of fresh pore fluid, or continued reaction with the host material. Within the shallow cryosphere--where oxygen can be accessed by microbes and microbes can be accessed by exploration-lithoautotrophs can function within as little as three monolayers of interfacial water formed either by adsorption from the atmosphere or in regions of ice stability where temperatures are within some tens of degrees of the ice melting point. For the selected reference host material (shergottite analog) and associated inorganic fluid chemistry, complete local reaction of the host material potentially yields a time-integrated biomass of approximately 0.1 mg of dry biomass/g of host material ( approximately 10(9) cells/g). Biomass could also be sustained where solutes can be delivered by advection (cryosuction) or diffusion in interfacial water; however, both of these processes are relatively inefficient. Lithoautotrophs in near-surface thin films of water, therefore, would optimize their metabolism by deriving energy and nutrients locally. Although the selected chemistry and associated model output indicate that lithoautotrophic microbial biomass could accrue within shallow interfacial water on Mars, it is likely that these organisms would spend long periods in maintenance or survival modes, with instantaneous biomass comparable to or less than that observed in extreme environments on Earth.     

A hydrothermal system associated with the Siljan impact structure, Sweden--implications for the search for fossil life on Mars

The Siljan ring structure (368 +/- 1.1 Ma) is the largest known impact structure in Europe. It isa 65-km-wide, eroded, complex impact structure, displaying several structural units, including a central uplifted region surrounded by a ring-shaped depression. Associated with the impact crater are traces of a post-impact hydrothermal system indicated by precipitated and altered hydrothermal mineral assemblages. Precipitated hydrothermal minerals include quartz veins and breccia fillings associated with granitic rocks at the outer margin of the central uplift, and calcite, fluorite, galena, and sphalerite veins associated with Paleozoic carbonate rocks located outside the central uplift. Two-phase water/gas and oil/gas inclusions in calcite and fluorite display homogenization temperatures between 75 degrees C and 137 degrees C. With an estimated erosional unloading of approximately 1 km, the formation temperatures were probably not more than 10-15 degrees C higher. Fluid inclusion ice-melting temperatures indicate a very low salt content, reducing the probability that the mineralization was precipitated during the Caledonian Orogeny. Our findings suggest that large impacts induce low-temperature hydrothermal systems that may be habitats for thermophilic organisms. Large impact structures on Mars may therefore be suitable targets in the search for fossil thermophilic organisms.     

Searching for life on Mars: selection of molecular targets for ESA's aurora ExoMars mission

The European Space Agency's ExoMars mission will seek evidence of organic compounds of biological and non-biological origin at the martian surface. One of the instruments in the Pasteur payload may be a Life Marker Chip that utilizes an immunoassay approach to detect specific organic molecules or classes of molecules. Therefore, it is necessary to define and prioritize specific molecular targets for antibody development. Target compounds have been selected to represent meteoritic input, fossil organic matter, extant (living, recently dead) organic matter, and contamination. Once organic molecules are detected on Mars, further information is likely to derive from the detailed distribution of compounds rather than from single molecular identification. This will include concentration gradients beneath the surface and gradients from generic to specific compounds. The choice of biomarkers is informed by terrestrial biology but is wide ranging, and nonterrestrial biology may be evident from unexpected molecular distributions. One of the most important requirements is to sample where irradiation and oxidation are minimized, either by drilling or by using naturally excavated exposures. Analyzing regolith samples will allow for the search of both extant and fossil biomarkers, but sequential extraction would be required to optimize the analysis of each of these in turn.     

“好奇”号登陆火星——奔向“盖尔”的梦幻舞步

2012年8月6日,“好奇”号在经过8个多月的星途旅行后,成功登陆火星,开始火星探测工作。作为美国第一辆采用核动力驱动的火星车,“好奇”号开启了一个新的火星探索时代。     

A microbial oasis in the hypersaline Atacama subsurface discovered by a life detector chip: implications for the search for life on Mars

The Atacama Desert has long been considered a good Mars analogue for testing instrumentation for planetary exploration, but very few data (if any) have been reported about the geomicrobiology of its salt-rich subsurface. We performed a Mars analogue drilling campaign next to the Salar Grande (Atacama, Chile) in July 2009, and several cores and powder samples from up to 5?m deep were analyzed in situ with LDChip300 (a Life Detector Chip containing 300 antibodies). Here, we show the discovery of a hypersaline subsurface microbial habitat associated with halite-, nitrate-, and perchlorate-containing salts at 2?m deep. LDChip300 detected bacteria, archaea, and other biological material (DNA, exopolysaccharides, some peptides) from the analysis of less than 0.5?g of ground core sample. The results were supported by oligonucleotide microarray hybridization in the field and finally confirmed by molecular phylogenetic analysis and direct visualization of microbial cells bound to halite crystals in the laboratory. Geochemical analyses revealed a habitat with abundant hygroscopic salts like halite (up to 260?g kg(-1)) and perchlorate (41.13?μg g(-1) maximum), which allow deliquescence events at low relative humidity. Thin liquid water films would permit microbes to proliferate by using detected organic acids like acetate (19.14?μg g(-1)) or formate (76.06?μg g(-1)) as electron donors, and sulfate (15875?μg g(-1)), nitrate (13490?μg g(-1)), or perchlorate as acceptors. Our results correlate with the discovery of similar hygroscopic salts and possible deliquescence processes on Mars, and open new search strategies for subsurface martian biota. The performance demonstrated by our LDChip300 validates this technology for planetary exploration, particularly for the search for life on Mars.     

Strategic, technological and ethical aspects of establishing colonies on Moon and Mars

With the vast experience gained by Aerospace Community in the last five decades, the natural future course of action will be to expand Space Exploration. Our understanding of Moon is relatively better with a number of unmanned satellite missions carried out by the leading Space Agencies and manned missions to Moon by USA. Also a number of unmanned satellite missions and surface rover missions were carried out to Mars by those Space agencies generating many new details about Mars. While the future exploration efforts by global community will also be centered obviously on Moon and Mars, it is noteworthy that already NASA had declared its plans for establishing a Surface Base on Moon and developing the technical infrastructure required. Surface Bases on Moon and Mars give rise to a number of strategic, technical and ethical issues both in the process of development, and in the process of establishing the bases. The strategic issues related to Moon and Mars Surface Bases will be centered around development of enabling technologies, cost of the missions, and international cooperation. The obvious path for tackling both the technological development and cost issues will be through innovative and new means of international cooperation. International cooperation can take many forms like—all capable players joining a leader, or sharing of tasks at system level, or all players having their independent programmes with agreed common interfaces of the items being taken to and left on the surface of Moon/Mars. Each model has its own unique features. Among the technical issues, the first one is that of the Mission Objectives—why Surface Bases have to be developed and what will be the activity of crew on Surface Bases? Surface Bases have to meet mainly the issues on long term survivability of humans on the Mars/Moon with their specific atmosphere, gravity and surface characteristics. Moon offers excellent advantages for astronomy while posing difficulties with respect to solar power utilization and extreme temperature variations. Hence the technical challenges depend on a number of factors starting from mission requirements. Obviously the most important technical challenge to be addressed will be in the areas of crew safety, crew survivability, adequate provision to overcome contingencies, and in-situ resource utilization. Towards this, new innovations will be developed in areas such as specialized space suits, rovers, power and communication systems, and ascent and descent modules. The biggest ethical issue is whether humankind from Earth is targeting ‘habitation’ or ‘colonization’ of Moon/Mars. The next question will be whether the in-situ resource exploitation will be only for carrying out further missions to other planets from Moon/Mars or for utilization on Earth. The third ethical issue will be the long term impact of pollution on Moon/Mars due to technologies employed for power generation and other logistics on Surfaces. The paper elaborates the views of the authors on the strategic, technical and ethical aspects of establishing Surface Bases and colonies on Moon and Mars. The underlying assumptions and gray areas under each aspect will be explained with the resulting long-term implications.     

Ultrastructural study of iron oxide precipitates: implications for the search for biosignatures in the Meridiani hematite concretions, Mars

Two terrestrial environments that have been proposed as analogs for the iron oxide precipitation in the Meridiani Planum region of Mars include the Rio Tinto precipitates and southern Utah marble concretions. Samples of two typical Utah iron oxide concretions and iron oxide precipitates in contact with biofilms from Rio Tinto have been studied to determine whether evidence could be found for biomediation in the precipitation process and to identify likely locations for fossil microorganisms. Scanning electron microscopy, energy dispersive X-ray, and gas chromatography-mass spectrometry (GC-MS) were used to search for biosignatures in the Utah marbles. The precipitation of iron oxides resembles known biosignatures, though organic compounds could not be confirmed with GC-MS analysis. In contrast, textural variations induced by biological activity are abundant in the modern Rio Tinto samples. Although no compelling evidence of direct or indirect biomediation was found in the Utah marbles, the ultrastructure of the iron oxide cement in the concretion suggests an inward growth during concretion precipitation from an initially spherical redox front. No indication for growth from a physical nucleus was found.     

Patent Analysis and Research on Mars Sampling Technology

This article reviews Mars sampling and return technology,by retrieving and analyzing the patents of domestic and foreign sampling equipment and mechanism techno...     

NASA宣布在月球和火星上发现水

NASA于9月24日宣布其科学家已在月球极区发现了水分子,并宣布在火星中纬度地区新形成的陨石坑内发现了水冰。这两项发现发表在次日出版的《科学》杂志上。     

火星移动智能体技术探讨

在火星表面巡视探测任务中,环境的复杂性、火地之间遥远的距离和探测器的安全性要求,决定了火星车必须具有较强的自主能力,自主实现环境感知、路径规划、科学探测、故障诊断等功能。移动智能体技术的发展和应用,为上述功能的更好实现提供了可能。文章通过对火星车自主能力的发展历程进行调研,结合当前我国巡视器技术的发展状况,从任务规划、能源管理、热控管理、通信管理等方面对火星移动智能体技术的应用需求进行了分析,提出了一种采用三层递阶式体系结构的解决方案框架,可为火星乃至其他地外天体移动智能体的技术实现提供参考。     

Water related environment modelling on Mars

During a human Mars exploration because of the lack of time astronauts need fast methods for the interpretation of unexpected observations which give them flexibility and new, important targets. With in-situ modelling it is possible to get information on various past and present processes at the same location on a far wider spectrum than would be realized even during a long mission. This work summarizes the potential technical requirements and benefits of the modelling. Based on a simple estimation with a 300 kg package, and 1-10% of the working time of 1-2 astronauts at the same location, they can get plenty of new and important information for the whole past and present Mars. With the proposed five test groups astronauts will be able to make better and newer kinds of interpretations of observations, and find better targets and methods during the same mission.     

中国志愿者王跃实验日志独家披露 “登陆”火星的心路历程

2011年2月2日这个世界总是有很多事情是因为巧合而赶到一起。今天是中国的除夕夜,也是我们打开EU50舱门的重要日子。大家都很激动——除了我。不晓得什么时候开始,当然我指的是进舱之后的变化了。很少,很少有能让我很激动很兴奋的事情了。即使今天是咱们中国人最重要的节日,似乎心情也只是淡淡的没有波澜。唯一有点儿好奇的是,EU50里面现在是个什么样子。答案是——塞满了。整个50立方米的登陆舱中塞满了物品,包括在厕所、床底、抽屉甚至地板下     

勇气号机遇号双双登陆火星

美国东部标准时间1月3日晚11时35分许，美国航宇局的勇气号火星探测器经过半年多的旅行，成功地降落在火星赤道以南15度的古谢夫环形山处。十几分钟后，它向地球发回了第一条信息。几小时后，它又传回了10多张着陆区附近的清晰照片。这标志着勇气号为期3个月的探测使命有了一个良好开端。     

拜访火星，中国10月启程

自1962年苏联发射首个火星探测器以来的近半个世纪里,世界各国先后发射火星探测器30多个。尽管这些“火星使者”中有2／3未能完成使命,但人类探测火星的热情却丝毫不减。尤其是新世纪以来,以美俄为主的航天大国,在火星探测方面又开始暗暗地较劲。已充分掌握航天技术的中国,在火星探测方面自然也希望有所建树。2009年10月,中国第一个火星探测器——“萤火一号”（YH-1）将搭乘俄罗斯的运载火箭,踏上拜访火星的漫漫旅程。