Microbial fuel cells applied to the metabolically based detection of extraterrestrial life

Since the 1970s, when the Viking spacecrafts carried out experiments to detect microbial metabolism on the surface of Mars, the search for nonspecific methods to detect life in situ has been one of the goals of astrobiology. It is usually required that a methodology detect life independently from its composition or form and that the chosen biological signature point to a feature common to all living systems, such as the presence of metabolism. In this paper, we evaluate the use of microbial fuel cells (MFCs) for the detection of microbial life in situ. MFCs are electrochemical devices originally developed as power electrical sources and can be described as fuel cells in which the anode is submerged in a medium that contains microorganisms. These microorganisms, as part of their metabolic process, oxidize organic material, releasing electrons that contribute to the electric current, which is therefore proportional to metabolic and other redox processes. We show that power and current density values measured in MFCs that use microorganism cultures or soil samples in the anode are much larger than those obtained with a medium free of microorganisms or sterilized soil samples, respectively. In particular, we found that this is true for extremophiles, which have been proposed as potential inhabitants of extraterrestrial environments. Therefore, our results show that MFCs have the potential to be used for in situ detection of microbial life.     

Can identification of a fourth domain of life be made from sequence data alone, and could it be done on Mars?

A central question in astrobiology is whether life exists elsewhere in the universe. If so, is it related to Earth life? Technologies exist that enable identification of DNA- or RNA-based microbial life directly from environmental samples here on Earth. Such technologies could, in principle, be applied to the search for life elsewhere; indeed, efforts are underway to initiate such a search. However, surveying for nucleic acid-based life on other planets, if attempted, must be carried out with caution, owing to the risk of contamination by Earth-based life. Here we argue that the null hypothesis must be that any DNA discovered and sequenced from samples taken elsewhere in the universe are Earth-based contaminants. Experience from studies of low-biomass ancient DNA demonstrates that some results, by their very nature, will not enable complete rejection of the null hypothesis. In terms of eliminating contamination as an explanation of the data, there may be value in identification of sequences that lie outside the known diversity of the three domains of life. We therefore have examined whether a fourth domain could be readily identified from environmental DNA sequence data alone. We concluded that, even on Earth, this would be far from trivial, and we illustrate this point by way of examples drawn from the literature. Overall, our conclusions do not bode well for planned PCR-based surveys for life on Mars, and we argue that other independent biosignatures will be essential in corroborating any claims for the presence of life based on nucleic acid sequences.     

A model of habitability within the Milky Way galaxy

We present a model of the galactic habitable zone (GHZ), described in terms of the spatial and temporal dimensions of the Galaxy that may favor the development of complex life. The Milky Way galaxy was modeled using a computational approach by populating stars and their planetary systems on an individual basis by employing Monte Carlo methods. We began with well-established properties of the disk of the Milky Way, such as the stellar number density distribution, the initial mass function, the star formation history, and the metallicity gradient as a function of radial position and time. We varied some of these properties and created four models to test the sensitivity of our assumptions. To assess habitability on the galactic scale, we modeled supernova rates, planet formation, and the time required for complex life to evolve. Our study has improved on other literature on the GHZ by populating stars on an individual basis and modeling Type II supernova (SNII) and Type Ia supernova (SNIa) sterilizations by selecting their progenitors from within this preexisting stellar population. Furthermore, we considered habitability on tidally locked and non-tidally locked planets separately and studied habitability as a function of height above and below the galactic midplane. In the model that most accurately reproduces the properties of the Galaxy, the results indicate that an individual SNIa is ～5.6× more lethal than an individual SNII on average. In addition, we predict that ～1.2% of all stars host a planet that may have been capable of supporting complex life at some point in the history of the Galaxy. Of those stars with a habitable planet, ～75% of planets are predicted to be in a tidally locked configuration with their host star. The majority of these planets that may support complex life are found toward the inner Galaxy, distributed within, and significantly above and below, the galactic midplane.     

SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.     

An Aristotelian account of minimal chemical life

This paper addresses the open philosophical and scientific problem of explaining and defining life. This problem is controversial, and there is nothing approaching a consensus about what life is. This raises a philosophical meta-question: Why is life so controversial and so difficult to define? This paper proposes that we can attribute a significant part of the controversy over life to use of a Cartesian approach to explaining life, which seeks necessary and sufficient conditions for being an individual living organism, out of the context of other organisms and the abiotic environment. The Cartesian approach contrasts with an Aristotelian approach to explaining life, which considers life only in the whole context in which it actually exists, looks at the characteristic phenomena involving actual life, and seeks the deepest and most unified explanation for those phenomena. The phenomena of life might be difficult to delimit precisely, but it certainly includes life's characteristic hallmarks, borderline cases, and puzzles. The Program-Metabolism-Container (PMC) model construes minimal chemical life as a functionally integrated triad of chemical systems, which are identified as the Program, Metabolism, and Container. Rasmussen diagrams precisely depict the functional definition of minimal chemical life. The PMC model illustrates the Aristotelian approach to life, because it explains eight of life's hallmarks, one of life's borderline cases (the virus), and two of life's puzzles.     

Metalaw and relations with intelligent beings revisited

A body of Metalaw must be recognized or established in order to anticipate the discovery of an incident of intelligent life indigenous to a place or dimension outside the realm and comprehension of extraterrestrial intelligence (ETI) as understood on the planet Earth. It has been said that the limits of your language are the limits of your world. Recognition of life and communication between intelligent beings is and will be the determining factor in humankind's world of the future. But what is the correct language to use in the search for ETI? Philosophical, legal and sociological realities will govern our future as beings of the universe and beyond. This is a study which must be pursued and perfected to the extent of our abilities prior to the immediacy of our need. Such study was begun many years ago by the best of our scholars but their activities are not well known today. In arguing for the need to proceed with our evolving discoveries of realities and possibilities, this article also brings to light the earlier work of jurists and philosophers on relationships with ETI.     

Space education policy — a global imperative

The discoveries and knowledge gained from space exploration and technology development are valuable scientific education tools which are not being adequately used in the classroom. Moreover, the increasing application of space technology to everyday life and industry requires a work force educated to be able to work productively in this field and to take advantage of all it has to offer. This article provides a survey of what is presently being done in the field of space education in Europe and the USA. Although encouraging, it is not nearly enough to meet the needs of 21st century society, principally because current efforts lack government or industry support. The author argues that this state of affairs must change, and proposed an international symposium as a first step towards this goal.     

Exosemiotics: an inter-disciplinary approach

Over the past several hundred years, many ideas have been expressed as to how human beings might communicate with extraterrestrials. These ideas have been put forth by experts from a diverse range of fields including physical scientists, mathematicians, behavioral scientists, philosophers and creative writers who have widely differing views on how to express ourselves coherently with civilizations from other worlds.This paper will look at some of these differing viewpoints and stress the need for an inter-disciplinary approach to the challenges of sending and, perhaps most important, receiving messages. Could we decipher a message if we got one? Who is doing the listening and what advantages could an inter-disciplinary approach bring to these efforts? What are some inter-disciplinary approaches to sending messages to extraterrestrials?     

Invited Pesek lecture: Exploration rather than speculation–assembling the puzzle of potential life beyond Earth

Speculations about the existence of life beyond Earth are probably as old as mankind itself, but still there is no evidence – neither for its presence nor for its absence. Moreover, we neither know the necessary nor the sufficient conditions for life to emerge, sustain or evolve. The Drake equation famously quantifies our ignorance by writing the number of detectable civilizations as product of factors that get increasingly uncertain the further one goes to the right. As a result, the predictive power is poor, and it ultimately depends on the most uncertain factor. However, if we were able to derive a reasonable estimate, we would not need SETI experiments to tell us whether we are alone or not. What has changed substantially over human history is our ability to explore the Universe. Most significantly, radio transmission technology gives us the opportunity to communicate over interstellar distances, and we are now able to not only determine the population statistics of planets within the Milky Way, but even in principle to find biosignatures in their atmospheres. By finding life beyond Earth, we will learn how frequently it emerges. By finding signals from intelligent extra-terrestrial civilizations, we will get unprecedented insight into our biological, technological, and societal evolution. The Drake equation is not such a useful means for assessing the chances of success of SETI, but instead it provides the framework for using observational data in advancing towards understanding the origins of our existence and our role in the cosmos, and maybe to get a glimpse of our future.     

Ultraviolet-stimulated fluorescence and phosphorescence of aromatic hydrocarbons in water ice

A principal goal of astrobiology is to detect and inventory the population of organic compounds on extraterrestrial bodies. Targets of specific interest include the wealth of icy worlds that populate our Solar System. One potential technique for in situ detection of organics trapped in water ice matrices involves ultraviolet-stimulated emission from these compounds. Here, we report a preliminary investigation into the feasibility of this concept. Specifically, fluorescence and phosphorescence of pure benzene ice and 1% mixtures of benzene, toluene, p-xylene, m-xylene, and o-xylene in water ice, respectively, were studied at temperatures ranging from ～17 K up to 160 K. Spectra were measured from 200-500 nm (50,000-20,000 cm(-1)) while ice mixtures were excited at 248.6 nm. The temperature dependence of the fluorescence and phosphorescence intensities was found to be independent of the thermal history and phase of the ice matrix in all cases examined. All phosphorescent emissions were found to decrease in intensity with increasing temperature. Similar behavior was observed for fluorescence in pure benzene, while the observed fluorescence intensity in water ices was independent of temperature.     

Is space an environment?

Expanding the human sphere of influence beyond Earth presents philosophical questions that also have important practical applications. Do we need to worry about the moral implications of our actions in the vastness of space? What kind of explorers will we be - and what kind of explorers should we be? The answers to these basic questions depend greatly on what moral status is assigned to space; how it is conceptualized. This article sets forth arguments both for and against considering space as an environment, that is, as a place deserving of ethical treatment in the same way that terrestrial environments are valued and respected in environmental ethics. It sketches some answers to how space exploration could meet high ethical standards and puts forward the notion of environmental ‘virtue ethics’.     

改进粒子滤波的弱目标跟踪与检测

基于粒子滤波的检测前跟踪技术（TBD\|PF）是低信噪比环境下目标检测与跟踪的有效方法。针对传统的TBD\|PF算法经重采样后容易导致粒子耗尽,从而跟丢目标的缺点。本文提出采用对权重较低的部分“存活”粒子用“新生”粒子将其替换,且对重采样后的粒子实施马尔科夫链蒙特卡洛（MCMC）移动步骤的粒子更新策略,在增加粒子多样性的同时保证了粒子的有效性。仿真实验分析了粒子数大小,预设门限的选取对检测性能的影响。实验结果表明,所提算法的检测与跟踪性能要优于传统的粒子滤波算法。
     

The NASA astrobiology program

The new discipline of astrobiology addresses fundamental questions about life in the universe: "Where did we come from?" "Are we alone in the universe?" "What is our future beyond the Earth?" Developing capabilities in biotechnology, informatics, and space exploration provide new tools to address these old questions. The U.S. National Aeronautics and Space Administration (NASA) has encouraged this new discipline by organizing workshops and technical meetings, establishing a NASA Astrobiology Institute, providing research funds to individual investigators, ensuring that astrobiology goals are incorporated in NASA flight missions, and initiating a program of public outreach and education. Much of the initial effort by NASA and the research community was focused on determining the technical content of astrobiology. This paper discusses the initial answer to the question "What is astrobiology?" as described in the NASA Astrobiology Roadmap.     

Managing Temporal Constraints with Preferences

Abstract

Preferences in temporal problems are common but significant in many real world applications. In this paper, we extend our temporal reasoning framework, managing numeric and symbolic information, in order to handle preferences. Unlike the existing models managing single temporal preferences, ours supports four types of preferences, namely: numeric and symbolic temporal preferences, composite preferences and conditional preferences. This offers more expressive power in representing a wide variety of temporal constraint problems. The preferences are considered here as a set of soft constraints using a c-semiring structure with combination and projection operators. Solving temporal constraint problems with preferences consists in finding a solution satisfying all the temporal constraints while optimizing the preference values. This is handled by a variant of the branch and bound algorithm, we propose in this paper, and where constraint propagation is used to improve the time efficiency. Experimental tests, we conducted on randomly generated temporal constraint problems with preferences, favor a variant of MAC as the constraint propagation strategy that should be used within the branch and bound algorithm.     

Predictive Maintenance of Manned Spacecraft Through Remaining Useful Life Estimation Technique

Manned spacecraft pose challenges in terms of extremely high safety and reliability, and with the growth of system complexity and longer on-orbit operation time, the traditional management mode, such as monitoring the threshold of parameter passively, is difficult to meet the required safety standards. Predictive maintenance, which analyzes the system heath trend and estimates remaining useful life(RUL) to establish maintenance strategies ahead of time before failure occurs, is a new mode to approach maintenance tasks. Here, a predictive maintenance strategy for complex manned spacecraft is proposed based on the remaining useful life estimation technique. Firstly, a health index is established based on an abundance of telemetry data, reflecting the system's current health state. Secondly, we map the health index to the remaining useful life through system degradation modelling, taking into consideration both the system's stochastic deterioration and uncertainty. The maintenance and management strategies are then made based on the calculated distribution of RUL time. Finally, a case study on Chinese space station energy system predictive maintenance is presented.     

MIMO信道空时联合功率优化

文章给出了评价MIMO信道质量的空域和时域准则,分别以MIMO系统各态历经容量最大化和中断容量最大化为目标,提出了空时联合的功率优化算法。对于各态历经容量最大化,采用空域和时域联合注水;对于中断容量最大化,在空域采用注水,时域上根据信道质量,仅分配满足一定中断概率要求的发射功率。仿真结果表明,空时联合优化与传统的空域注水得到的各态历经容量几乎相同,但是,引入时域自适应能够显著改善系统的中断特性。     

火星探测的主要科学问题

在太阳系的行星中,火星与地球之间存在最多的相似之处,因此,火星是一颗承载人类最多梦想的星球。火星有水和生命存在的问题,激发了人类火星探索的好奇心,成为人类持续探测火星的推动力。火星的起源和演化与太阳系形成过程的关系,火星与类地行星的共性和特性,是当代行星科学研究的重要内容。为了人类社会的可持续发展,火星可否改造成为适宜人类居住的绿色星球——这些是人类在火星探测中必须面对的重大科学问题。只有这些重大科学问题被一一解答,我们才能清晰地去思考地球和人类自身的未来！     

India's EO infrastructure for disaster reduction: Lessons and perspectives

India has established a ‘critical mass’ in terms of EO infrastructure for disaster management. Starting from IRS 1A in 1980s to the most recent CARTOSAT-2, India's EO series of satellites are moving away from the generic to thematic constellations. The series of RESOURCESAT, CARTOSAT, OCEANSAT and forthcoming Radar Imaging Satellite (RISAT) satellites exemplifies the thematic characters of the EO missions. These thematic constellations, characterized with multi-platform, multi-resolution and multi-parameter EO missions, are important assets for disaster reduction. In the more specific term, these constellations in conjunction with contemporary EO missions address the critical observational gaps in terms of capturing the catastrophic events, phenomena or their attributes on real/near real time basis with appropriate spatial and temporal attributes.Using conjunctively the data primarily emanating these thematic constellations and all weather radar data from aerial platform and also from RADARSAT as gap-fillers has been a part of India's EO strategy for disaster management. The infrastructure has been addressing the observational needs in disaster management. The high resolution imaging better than one-meter spatial resolution and also Digital Elevation Models (DEM) emanating from Cartosat series are providing valuable inputs to characterize geo-physical terrain vulnerability. Radar Imaging Satellite, with all weather capability missions, is being configured for disaster management. At present, the current Indian EO satellites cover the whole world every 40 h (with different resolutions and swaths), and the efforts are towards making it better than 24 h. The efforts are on to configure RESOURCESAT 3 with wider swath of 740 km with 23 m spatial resolution and also to have AWiFS type of capability at geo-platform to improve the observational frequencies for disaster monitoring.India's EO infrastructure has responded comprehensively to all the natural disasters the country has faced in the recent times. As a member of International Charter on Space and Major Disasters, India has also been instrumental in promoting the related UN initiatives viz., RESAP of UN ESCAP, SPIDER of UN OOSA, Sentinel Asia of JAXA initiative and also of GEOSS initiative. The paper intends to illustrate India's EO strategy for disaster reduction.     

对太阳能源由来的机理分析（英文）

Why has the sun burnt for billions of years? What is the mechanism of combustion? What will happen to the trend of solar combustion? Modern humans have only just begun their close-up exploration of the sun. By studying the sun, we can learn more about other stars. The more we understand other stars, the more we will know about the universe in the end. The exploration of the origin of solar energy will help people to deepen their understanding of the origin of the universe as well as the development of the universe. Solar probes will help us further understand the characteristics of the sun, validate relevant theories and make new discoveries.