Comparison of aerobically-treated and untreated crop residue as a source of recycled nutrients in a recirculating hydroponic system.

This study compared the growth of potato plants on nutrients recycled from inedible potato biomass. Plants were grown for 105 days in recirculating, thin-film hydroponic systems containing four separate nutrient solution treatments: (1) modified half-strength Hoagland's (control), 2) liquid effluent from a bioreactor containing inedible potato biomass, 3) filtered (0.2 micrometer) effluent, and 4) the water soluble fraction of inedible potato biomass (leachate). Approximately 50% of the total nutrient requirement in treatments 2-4 were provided (recycled) from the potato biomass. Leachate had an inhibitory effect on leaf conductance, photosynthetic rate, and growth (50% reduction in plant height and 60% reduction in tuber yield). Plants grown on bioreactor effluent (filtered or unfiltered) were similar to the control plants. These results indicated that rapidly degraded, water soluble organic material contained in the inedible biomass, i.e., material in leachate, brought about phytotoxicity in the hydroponic culture of potato. Recalcitrant, water soluble organic material accumulated in all nutrient recycling treatments (650% increase after 105 days), but no increase in rhizosphere microbial numbers was observed.     

Use of biologically reclaimed minerals for continuous hydroponic potato production in a CELSS.

Plant-derived nutrients were successfully recycled in a Controlled Ecological Life Support System (CELSS) using biological methods. The majority of the essential nutrients were recovered by microbiologically treating the plant biomass in an aerobic bioreactor. Liquid effluent containing the nutrients was then returned to the biomass production component via a recirculating hydroponic system. Potato (Solanum tuberosum L.) cv. Norland plants were grown on those nutrients in either a batch production mode (same age plants on a nutrient solution) or a staggered production mode (4 different ages of plants on a nutrient solution). The study continued over a period of 418 days, within NASA Breadboard Project's Biomass Production Chamber at the Kennedy Space Center. During this period, four consecutive batch cycles (104-day harvests) and 13 consecutive staggered cycles (26-day harvests) were completed using reclaimed minerals and compared to plants grown with standard nutrient solutions. All nutrient solutions were continually recirculated during the entire 418 day study. In general, tuber yields with reclaimed minerals were within 10% of control solutions. Contaminants, such as sodium and recalcitrant organics tended to increase over time in solutions containing reclaimed minerals, however tuber composition was comparable to tubers grown in the control solutions.     

Growth of sweetpotato cultured in the newly designed hydroponic system for space farming

Life support of crews in long-duration space missions for other planets will be highly dependent on amounts of food, atmospheric O₂ and clean water produced by plants. Therefore, the space farming system with scheduling of crop production, obtaining high yields with a rapid turnover rate, converting atmospheric CO₂ to O₂ and purifying water should be established with employing suitable plant species and cultivars and precisely controlling environmental variables around plants grown at a high density in a limited space. In this study, we developed a new hydroponic method for producing tuberous roots and fresh edible leaves and stems of sweetpotato. In the first experiment, we examined the effects of water contents in the rooting substrate on growth and tuberous root development of sweetpotato. The rooting substrates made with rockwool slabs were inclined in a culture container and absorbed nutrient solution from the lower end of the slabs by capillary action. Tuberous roots developed on the lower surface of the rockwool slabs. The tuberous roots showed better growth and development at locations farther from the water surface on the rockwool slabs, which had lower water content. In the second experiment, three sweetpotato cultivars were cultured in a hydroponic system for five months from June to November under the sun light in Osaka, Japan as a fundamental study for establishing the space farming system. The cultivars employed were ‘Elegant summer’, ‘Kokei-14’ and ‘Beniazuma’. The hydroponic system mainly consisted of culture containers and rockwool slabs. Dry weights of tuberous roots developed in the aerial space between the rockwool slab and the nutrient solution filled at the bottom of the culture container were 0.34, 0.45 and 0.23 kg/plant and dry weights of the top portion (leaves, petioles and stems) were 0.42, 0.29 and 0.61 kg/plant for ‘Elegant summer’, ‘Kokei-14’ and ‘Beniazuma’, respectively. Young stems and leaves as well as tuberous roots of ‘Elegant summer’ are edible and palatable. Therefore ‘Elegant summer’ would be a promising crop to produce large amounts of food with high nutritional values in the present hydroponic system in space farming.     

Effects of several environmental factors on sweetpotato growth.

Effects of relative humidity, light intensity and photoperiod on growth of 'Ga Jet' and 'TI-155' sweetpotato cultivars, using the nutrient film technique (NFT), have been reported. In this study, the effect of ambient temperature regimes (constant 28 degrees C and diurnal 28:22 degrees C day:night) and different CO2 levels (ambient, 400, 1000 and 10000 microliters/L--400, 1000 and 10000 ppm) on growth of one or both of these cultivars in NFT are reported. For a 24-h photoperiod, no storage roots were produced for either cultivar in NFT when sweetpotato plants were grown at a constant temperature of 28 degrees C. For the same photoperiod, when a 28:22 degrees C diurnal temperature variation was used, there were still no storage roots for 'TI-155' but the cv. 'Ga Jet' produced 537 g/plant of storage roots. For both a 12-h and 24-h photoperiod, 'Ga Jet' storage root fresh and dry weight tended to be higher with a 28:22 degrees C diurnal temperature variation than with a constant 28 degrees C temperature regime. Preliminary results with both 'Ga Jet' and 'TI 155' cultivars indicate a distinctive diurnal stomatal response for sweetpotato grown in NFT under an ambient CO2 level. The stomatal conductance values observed for 'Ga Jet' at elevated CO2 levels indicated that the difference between the light- and dark-period conductance rates persisted at 400, 1000, and 10000 microliters/L.     

Integration of crop production with CELSS waste management.

Lettuce plants were grown utilizing water, inorganic elements, and CO2 inputs recovered from waste streams. The impact of these waste-derived inputs on the growth of lettuce was quantified and compared with results obtained when reagent grade inputs were used. Phytotoxicity was evident in both the untreated wastewater stream and the recovered CO2 stream. The toxicity of surfactants in wastewater was removed using several treatment systems. Harmful effects of gaseous products resulting from incineration of inedible biomass on crop growth were observed. No phytotoxicity was observed when inorganic elements recovered from incinerated biomass ash were used to prepare the hydroponic solution, but the balance of nutrients had to be modified to achieve near optimal growth. The results were used to evaluate closure potential of water and inorganic elemental loops for integrated plant growth and human requirements.     

Evaluation of an anaerobic digestion system for processing CELSS crop residues for resource recovery.

Three bioreactors, connected in series, were used to process CELSS potato residues for recovery of resources. The first stage was an anaerobic digestor (8 L working volume; cow rumen contents inoculum; fed-batch; 8 day retention time; feed rate 25 gdw day-1) that converted 33% of feed (dry weight loss) to CO2 and "volatile fatty acids" (vfa, 83:8:8 mmolar ratio acetic:propionic:butyric). High nitrate-N in the potato residue feed was absent in the anaerobic effluent, with a high portion converted to NH4(+)-N and the remainder unaccounted and probably lost to denitrification and NH4+ volatilization. Liquid anaerobic effluent was fed to an aerobic, yeast biomass production vessel (2 L volume; Candida ingens inoculum; batch [pellicle] growth; 2 day retention time) where the VFAs and some NH4(+)-N were converted into yeast biomass. Yeast yields accounted for up to 8% of potato residue fed into the anaerobic bioreactor. The third bioreactor (0.5 L liquid working volume; commercial nitrifier inoculum; packed-bed biofilm; continuous yeast effluent feed; recirculating; constant volume; 23 day hydraulic retention time) was used to convert successfully the remaining NH4(+)-N into nitrate-N (preferred form of N for CELSS crop production) and to remove the remaining degradable soluble organic carbon. Effluents from the last two stages were used for partial replenishment of minerals for hydroponic potato production.     

Proximate composition of CELSS crops grown in NASA's Biomass Production Chamber.

Edible biomass from four crops of wheat (Triticum aestivum L.), four crops of lettuce (Lactuca sativa L.), four crops of potato (Solanum tuberosum L.), and three crops of soybean (Glycine max (L.) Merr.) grown in NASA's CELSS Biomass Production Chamber were analyzed for proximate composition. All plants were grown using recirculating nutrient (hydroponic) film culture with pH and electrical conductivity automatically controlled. Temperature and humidity were controlled to near optimal levels for each species and atmospheric carbon dioxide partial pressures were maintained near 100 Pa during the light cycles. Soybean seed contained the highest percentage of protein and fat, potato tubers and wheat seed contained the highest levels of carbohydrate, and lettuce leaves contained the highest level of ash. Analyses showed values close to data published for field-grown plants with several exceptions: In comparison with field-grown plants, wheat seed had higher protein levels; soybean seed had higher ash and crude fiber levels; and potato tubers and lettuce leaves had higher protein and ash levels. The higher ash and protein levels may have been a result of the continuous supply of nutrients (e.g., potassium and nitrogen) to the plants by the recirculating hydroponic culture.     

Sweetpotato vine management for confined food production in a space life-support system

Sweetpotato (Ipomea batatas L.) ‘Whatley–Loretan’ was developed for space life support by researchers at Tuskegee University for its highly productive, nutritious storage roots. This promising candidate space life-support crop has a sprawling habit and aggressive growth rate in favorable environments that demands substantial growing area. Shoot pruning is not a viable option for vine control because removal of the main shoot apex drastically inhibits storage-root initiation and development, and chemical growth retardants typically are not cleared for use with food crops. As part of a large effort by the NASA Specialized Center of Research and Training in Advanced Life Support to reduce equivalent system mass (ESM) for food production in space, the dilemma of vine management for sweetpotato was addressed in effort to conserve growth area without compromising root yield. Root yields from unbranched vines trained spirally around wire frames configured either in the shapes of cones or cylinders were similar to those from vines trained horizontally along the bench, but occupying only a small fraction of the bench area. This finding indicates that sweetpotato is highly adaptable to a variety of vine-training architectures. Planting a second plant in the growth container and training the two vines in opposite directions around frames enhanced root yield and number, but had little effect on average length of each vine or bench area occupied. Once again, root yields were similar for both configurations of wire support frames. The 3–4-month crop-production cycles for sweetpotato in the greenhouse spanned all seasons of multiple years during the course of the study, and although electric lighting was used for photoperiod control and to supplement photosynthetic light during low-light seasons, there still were differences in total light available across seasons. Light variations and other environmental differences among experiments in the greenhouse had more effects on vine length than on root yield. Average vine length correlated positively with total hours of daylight received across seasons, and responses for one plant per container were higher above a threshold duration of solar exposure, suggesting that the vines of two plants per container compete for available light. In addition to the adaptability of sweetpotato to various vine-training architectures and across seasons in terms of maintaining root productivity, the open, interior volumes of the support frames tested in this study will provide future opportunity to enhance sweetpotato root yield in space by adding novel interior lighting, such as from intracanopy arrays of light-emitting diodes. This work was sponsored by NASA grant NAG 5 1286.     

Selection and hydroponic growth of potato cultivars for bioregenerative life support systems

As part of the ESA-funded MELiSSA program, Ghent University and the Université catholique de Louvain investigated the suitability, growth and development of four potato cultivars in hydroponic culture under controlled conditions with the aim to incorporate such cultivation system in an Environmental Control and Life Support System (ECLSS). Potato plants can fulfill three major functions in an ECLSS in space missions: (a) fixation of CO₂ and production of O₂, (b) production of tubers for human nutrition and (c) production of clean water after condensation of the water vapor released from the plants by transpiration. Four cultivars (Annabelle, Bintje, Desiree and Innovator) were selected and grown hydroponically in nutrient film technique (NFT) gullies in a growth chamber under controlled conditions. The plant growth parameters, tuber harvest parameters and results of tuber nutritional analysis of the four cultivars were compared. The four potato cultivars grew well and all produced tubers. The growth period lasted 127 days for all cultivars except for Desiree which needed 145 days. Annabelle (1.45 kg/m²) and Bintje (1.355 kg/m²) were the best performing of the four cultivars. They also produced two times more tubers than Desiree and Innovator. Innovator produced the biggest tubers (20.95 g/tuber) and Desiree the smallest (7.67 g/tuber). The size of Annabelle and Bintje potatoes were intermediate. Bintje plants produced the highest total biomass in term of DW. The highest non-edible biomass was produced by Desiree, which showed both the highest shoot and root DW. The manual length and width measurements were also used to predict the total tuber mass. The energy values of the tubers remained in the range of the 2010 USDA and Souci-Fachmann-Kraut food composition databases. The amount of Ca determined was slightly reduced compared to the USDA value, but close to the Souci-Fachmann-Kraut value. The concentration of Cu, Zn and P were high compared to both databases.     

Soybean cultivar selection for Bioregenerative Life Support Systems (BLSSs) – Hydroponic cultivation

Four soybean cultivars (‘Atlantic’, ‘Cresir’, ‘Pr91m10’ and ‘Regir’), selected through a theoretical procedure as suitable for cultivation in BLSS, were evaluated in terms of growth and production. Germination percentage and Mean Germination Time (MGT) were measured. Plants were cultivated in a growth chamber equipped with a recirculating hydroponic system (Nutrient Film Technique). Cultivation was performed under controlled environmental conditions (12 h photoperiod, light intensity 350 μmol m⁻² s⁻¹, temperature regime 26/20 °C light/dark, relative humidity 65–75%). Fertigation was performed with a standard Hoagland solution, modified for soybean specific requirements, and EC and pH were kept at 2.0 dS m⁻¹ and 5.5 respectively.     

Excess nutrients in hydroponic solutions alter nutrient content of rice, wheat, and potato.

Environment has significant effects on the nutrient content of field-grown crop plants. Little is known, however, about compositional changes caused by controlled environments in which plants receive only artificial radiation and soilless, hydroponic culture. This knowledge is essential for developing a safe, nutritious diet in a Controlled Ecological Life-Support System (CELSS). Three crops that are candidates for inclusion in a CELSS (rice, wheat, and white potato) were grown both in the field and in controlled environments where the hydroponic nutrient solution, photosynthetic photon flux (PPF), and CO2 level were manipulated to achieve rapid growth rates. Plants were harvested at maturity, separated into discrete parts, and dried prior to analysis. Plant materials were analyzed for proximate composition (protein, fat, ash, and carbohydrate), total nitrogen (N), nitrate, minerals, and amino-acid composition. The effect of environment on nutrient content varied by crop and plant part. Total N and nonprotein N (NPN) contents of plant biomass generally increased under controlled-environment conditions compared to field conditions, especially for leafy plant parts and roots. Nitrate levels were increased in hydroponically-grown vegetative tissues, but nitrate was excluded from grains and tubers. Mineral content changes in plant tissue included increased phosphorus and decreased levels of certain micronutrient elements under controlled-environment conditions. These findings suggest that cultivar selection, genetic manipulation, and environmental control could be important to obtain highly nutritious biomass in a CELSS.     

Differentiation of cartilaginous anlagen in entire embryonic mouse limbs cultured in a rotating bioreactor.

Mechanisms involved in development of the embryonic limb have remained the same throughout eons of genetic and environmental evolution under Earth gravity (1 g). During the spaceflight era it has been of interest to explore the ancient theory that form of the skeleton develops in response to gravity, and that changes in gravitational forces can change the developmental pattern of the limb. This has been shown in vivo and in vitro, allowing the hypergravity of centrifugation and microgravity of space to be used as tools to increase our knowledge of limb development. In recapitulations of spaceflight experiments, premetatarsals were cultured in suspension in a bioreactor, and found to be shorter and less differentiated than those cultured in standard culture dishes. This study only measured length of the metatarsals, and did not account for possible changes due to the skeletal elements having a more in vivo 3D shape while in suspension vs. flattened tissues compressed by their own weight. A culture system with an outcome closer to in vivo and that supports growth of younger limb buds than traditional systems will allow studies of early Hox gene expression, and contribute to the understanding of very early stages of development. The purpose of the current experiment was to determine if entire limb buds could be cultured in the bioreactor, and to compare the growth and differentiation with that of culturing in a culture dish system. Fore and hind limbs from E11-E13 ICR mouse embryos were cultured for six days, either in the bioreactor or in center-well organ culture dishes, fixed, and embedded for histology. E13 specimens grown in culture dishes were flat, while bioreactor culture specimens had a more in vivo-like 3D limb shape. Sections showed excellent cartilage differentiation in both culture systems, with more cell maturation, and hypertrophy in the specimens cultured in the bioreactor. Younger limb buds fused together during culture, so an additional set of E11.5 limb buds was cultured with and without encapsulation in alginate prior to culturing in the bioreactor. Encapsulated limbs grown in the bioreactor did not fuse together, but developed only the more proximal elements while limbs grown in culture dishes formed proximal and distal elements. Alginate encapsulation may have reduced oxygenation to the progress zone of the developing limb bud resulting in lack of development of the more distal elements. These results show that the bioreactor supports growth and differentiation of skeletal elements in entire E13 limb buds, and that a method to culture younger limb buds without fusing together needs to be developed if any morphometric analysis is to be performed.     

Effect of lamp type and temperature on development, carbon partitioning and yield of soybean.

Soybeans grown in controlled environments are commonly taller than field-grown plants. In controlled environments, including liquid hydroponics, height of the dwarf cultivar "Hoyt" was reduced from 46 to 33 cm when plants were grown under metal halide lamps compared to high pressure sodium lamps at the same photosynthetic photon flux. Metal halide lamps reduced total biomass 14% but did not significantly reduce seed yield. Neither increasing temperature nor altering the difference between day/night temperature affected plant height. Increasing temperature from 21 to 27 degrees C increased yield 32%. High temperature significantly increased carbon partitioning to stems and increased harvest index.     

Effect of CO2 levels on nutrient content of lettuce and radish.

Atmospheric carbon-dioxide enrichment is known to affect the yield of lettuce and radish grown in controlled environments, but little is known about CO2 enrichment effects on the chemical composition of lettuce and radish. These crops are useful model systems for a Controlled Ecological Life-Support System (CELSS), largely because of their relatively short production cycles. Lettuce (Lactuca sativa L.) cultivar 'Waldmann's Green' and radish (Raphanus sativus L.) cultivar 'Giant White Globe' were grown both in the field and in controlled environments, where hydroponic nutrient solution, light, and temperature were regulated, and where CO2 levels were controlled at 400, 1000, 5000, or 10,000 ppm. Plants were harvested at maturity, dried, and analyzed for proximate composition (protein, fat, ash, and carbohydrate), total nitrogen (N), nitrate N, free sugars, starch, total dietary fiber, and minerals. Total N, protein N, nonprotein N (NPN), and nitrate N generally increased for radish roots and lettuce leaves when grown under growth chamber conditions compared to field conditions. The nitrate-N level of lettuce leaves, as a percentage of total NPN, decreased with increasing levels of CO2 enrichment. The ash content of radish roots and of radish and lettuce leaves decreased with increasing levels of CO2 enrichment. The levels of certain minerals differed between field- and chamber-grown materials, including changes in the calcium (Ca) and phosphorus (P) contents of radish and lettuce leaves, resulting in reduced Ca/P ratio for chamber-grown materials. The free-sugar contents were similar between the field and chamber-grown lettuce leaves, but total dietary fiber content was much higher in the field-grown plant material. The starch content of growth-chamber lettuce increased with CO2 level.     

Soybean cultivation for Bioregenerative Life Support Systems (BLSSs): The effect of hydroponic system and nitrogen source

Soybean [Glycine max (L.) Merr.] is one of the plant species selected within the European Space Agency (ESA) Micro-Ecological Life Support System Alternative (MELiSSA) project for hydroponic cultivation in Biological Life Support Systems (BLSSs), because of the high nutritional value of seeds. Root symbiosis of soybean with Bradirhizobium japonicum contributes to plant nutrition in soil, providing ammonium through the bacterial fixation of atmospheric nitrogen. The aim of this study was to evaluate the effects of two hydroponic systems, Nutrient Film Technique (NFT) and cultivation on rockwool, and two nitrogen sources in the nutrient solution, nitrate (as Ca(NO₃)₂ and KNO₃) and urea (CO(NH₂)₂), on root symbiosis, plant growth and seeds production of soybean. Plants of cultivar ‘OT8914’, inoculated with B. japonicum strain BUS-2, were grown in a growth chamber, under controlled environmental conditions.     

Utilization of sweet potatoes in controlled ecological life support systems (CELSS).

A number of studies have selected the sweet potato as a potentially important crop for CELSS. Most hydroponic studies of sweet potatoes have been short term (<80 days). Full term (90 to 150 days) studies of sweet potatoes in hydroponic systems were needed to understand the physiology of storage root enlargement and to evaluate sweet potato production potential for CELSS. Early and late maturing sweet potato varieties were crown in hydroponic systems of different types--static with periodic replacement, flowing with and without recirculation, aggregate, and non-aggregate. In a flowing system with recirculation designed at Tuskegee University using the nutrient film technique (NFT), storage root yields as high as 1790 g were produced with an edible growth rate of up to 66 g m-2 d-1 and a harvest index as high as 89% under greenhouse conditions. Preliminary experiments indicated high yields can be obtained in controlled environmental chambers. Significant cultivar differences were found in all systems studied. Nutritive composition of storage roots and foliage were similar to field-grown plants. The results indicate great potential for sweet potato in CELSS.     

Selection and hydroponic growth of bread wheat cultivars for bioregenerative life support systems

As part of the ESA-funded MELiSSA program, the suitability, the growth and the development of four bread wheat cultivars were investigated in hydroponic culture with the aim to incorporate such a cultivation system in an Environmental Control and Life Support System (ECLSS). Wheat plants can fulfill three major functions in space: (a) fixation of CO₂ and production of O₂, (b) production of grains for human nutrition and (c) production of cleaned water after condensation of the water vapor released from the plants by transpiration. Four spring wheat cultivars (Aletsch, Fiorina, Greina and CH Rubli) were grown hydroponically and compared with respect to growth and grain maturation properties. The height of the plants, the culture duration from germination to harvest, the quantity of water used, the number of fertile and non-fertile tillers as well as the quantity and quality of the grains harvested were considered. Mature grains could be harvested after around 160 days depending on the varieties. It became evident that the nutrient supply is crucial in this context and strongly affects leaf senescence and grain maturation. After a first experiment, the culture conditions were improved for the second experiment (stepwise decrease of EC after flowering, pH adjusted twice a week, less plants per m²) leading to a more favorable harvest (higher grain yield and harvest index). Considerably less green tillers without mature grains were present at harvest time in experiment 2 than in experiment 1. The harvest index for dry matter (including roots) ranged from 0.13 to 0.35 in experiment 1 and from 0.23 to 0.41 in experiment 2 with modified culture conditions. The thousand-grain weight for the four varieties ranged from 30.4 to 36.7 g in experiment 1 and from 33.2 to 39.1 g in experiment 2, while market samples were in the range of 39.4–46.9 g. Calcium levels in grains of the hydroponically grown wheat were similar to those from field-grown wheat, while potassium, magnesium, phosphorus, iron, zinc, copper, manganese and nickel levels tended to be higher in the grains of experimental plants. It remains a challenge for future experiments to further adapt the nutrient supply in order to improve senescence of vegetative plant parts, harvest index and the composition of bread wheat grains.     

Hydroponic potato production on nutrients derived from anaerobically-processed potato plant residues.

Bioregenerative methods are being developed for recycling plant minerals from harvested inedible biomass as part of NASA's Advanced Life Support (ALS) research. Anaerobic processing produces secondary metabolites, a food source for yeast production, while providing a source of water soluble nutrients for plant growth. Since NH4-N is the nitrogen product, processing the effluent through a nitrification reactor was used to convert this to NO3-N, a more acceptable form for plants. Potato (Solanum tuberosum L.) cv. Norland plants were used to test the effects of anaerobically-produced effluent after processing through a yeast reactor or nitrification reactor. These treatments were compared to a mixed-N treatment (75:25, NO3:NH4) or a NO3-N control, both containing only reagent-grade salts. Plant growth and tuber yields were greatest in the NO3-N control and yeast reactor effluent treatments, which is noteworthy, considering the yeast reactor treatment had high organic loading in the nutrient solution and concomitant microbial activity.     

Significance of rhizosphere microorganisms in reclaiming water in a CELSS.

Plant-microbe interactions, such as those of the rhizosphere, may be ideally suited for recycling water in a Controlled Ecological Life Support System (CELSS). The primary contaminant of waste hygiene water will be surfactants or soaps. We identified changes in the microbial ecology in the rhizosphere of hydroponical1y grown lettuce during exposure to surfactant. Six week old lettuce plants were transferred into a chamber with a recirculating hydroponic system. Microbial density and population composition were determined for the nutrient solution prior to introduction of plants and then again with plants prior to surfactant addition. The surfactant Igepon was added to the recirculating nutrient solution to a final concentration of 1.0 g L-1. Bacteria density and species diversity of the solution were monitored over a 72-h period following introduction of Igepon. Nine distinct bacterial types were identified in the rhisosphere; three species accounted for 87% of the normal rhizosphere population. Microbial cell number increased in the presence of Igepon, however species diversity declined. At the point when Igepon was degraded from solution, diversity was reduced to only two species. Igepon was found to be degraded directly by only one species found in the rhizosphere. Since surfactants are degraded from the waste hygiene water within 24 h, the potential for using rhizosphere bacteria as a waste processor in a CELSS is promising.     

Density and composition of microorganisms during long-term (418 day) growth of potato using biologically reclaimed nutrients from inedible plant biomass.

A study evaluating alternative methods for long term operation of biomass production systems was recently completed at the Kennedy Space Center (KSC). The 418-day study evaluated repeated batch versus mixed-aged production of potato grown on either standard 1/2-strength Hoagland's nutrient solution or solutions including nutrients recycled from inedible plant material. The long term effects of closure and recycling on microbial dynamics were evaluated by monitoring the microbial communities associated with various habitats within the plant growth system (i.e., plant roots, nutrient solution, biofilms within the hydroponic systems, atmosphere, and atmospheric condensate). Plate count methods were used to enumerate and characterize microorganisms. Microscopic staining methods were used to estunate total cell densities. The primary finding was that the density and composition of microbial communities associated with controlled environmental plant growth systems are stable during long term operation. Continuous production resulted in slightly greater stability. Nutrient recycling, despite the addition of soluble organic material from the waste processing system, did not significantly increase microbial density in any of the habitats.