Biological modulation of the terrestrial carbon cycle: Isotope clues to early organic evolution

The characteristic difference of some 20 to 30 ‰ in the ¹³C/¹²C rations of reduced (organic) and oxidized (carbonate) carbon as observed in the contemporary environment can be traced back over 3.5 (if not 3.8) Ga of recorded Earth history. There is little doubt that the enrichment of isotopically light carbon in fossil organic matter ultimately derives from the bias in favor of ¹²C during photosynthetic carbon fixation (notably the RuBP carboxylase reaction of the Calvin cycle). Accordingly, the mainstream of the sedimentary δ ¹³C_org record can be most reasonably explained as geochemical evidence of the isotope-discriminating properties of the principal CO₂-fixing enzymatic reaction of the assimilatory pathway, thus giving eloquent testimony to an extreme degree of evolutionary conservatism in the biochemistry of autotrophic carbon fixation.     

Isotope fractionations in the terrestrial carbon cycle: a brief overview.

The bias in favour of isotopically light carbon inherent in biological carbon fixation has brought about an isotopic disproportionation of primordial (mantle-derived) carbon on a global scale, causing an enrichment of 12C in reduced (biogenic) carbon and a concomitant accumulation of the heavy complement (13C) in the residual oxidized (inorganic) carbon pool. As a result, the terrestrial carbon cycle has gone bipartite, comprising an organic branch of isotopically light carbon, and an inorganic branch made up of 13C-enriched carbon (mostly in the form of carbonate). The isotopic disparity between the two principal terrestrial carbon species can be traced back over 3.8 Gyr of Earth history, attesting to a biological modulation of the carbon cycle since the time of formation of the oldest sediments.     

Stable carbon isotope fractionation in the search for life on early Mars.

Isotopic measurements and, more specifically, ratios of 13C to 12C in organic relative to inorganic deposits, are useful in reconstructing past biological activity on Earth. Organic matter has a lower ratio of 13C to 12C due largely to the preferential fixation of 12C over the heavier isotope by the major carbon-fixation enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase, although other factors (e.g., availability of source carbon, fixation by other carboxylating enzymes and diagenesis of organic material) also contribute to fractionation. Would carbon isotope discrepancies between inorganic and organic carbon indicate past biological activity on Mars? In order to answer this question, we analyse what is known about terrestrial biologic and abiologic carbon fixation and its preservation in the fossil record, and suggest what the isotope discrimination during possible biologic and abiologic carbon fixation on Mars might have been like. Primarily because isotopic signatures of abiotically fixed carbon overlap with those of biotic fixation, but also because heterotrophy does not significantly alter the isotopic signature of ingested carbon, fractionation alone would not be definitive evidence for life. However, a narrow range of fractionation, including no fractionation, would suggest biotic processes. Never-the-less, isotopic ratios in organic deposits on Mars would be extremely useful in analysing prebiotic, if not biotic, carbon transformations on Mars.     

How much of the solar irradiance variations is caused by the magnetic field at the solar surface?

The contribution to total solar irradiance variations by the magnetic field at the solar surface is estimated. Detailed models of the irradiance changes on the basis of magnetograms show that magnetic features at the solar surface account for over 90% of the irradiance variations on a solar rotation time scale and at least 70% on a solar cycle time scale. If the correction to the VIRGO record proposed by Fröhlich & Finsterle (2001) is accepted, then magnetic features at the solar surface are responsible for over 90% of the solar cycle irradiance variations as well.     

Elevated CO2: impact on diurnal patterns of photosynthesis in natural microbial ecosystems.

Algae, including blue-green algae (cyanobacteria), are the major source of fixed carbon in many aquatic ecosystems. Previous work has shown that photosynthetic carbon fixation is often enhanced in the presence of additional carbon dioxide (CO2). This study was undertaken to determine if this CO2 fertilization effect extended to microbial mats, and, if so, at what times during the day might the addition of CO2 affect carbon fixation. Four microbial mats from diverse environments were selected, including mats from a hypersaline pond (area 5, Exportadora de Sal, Mexico), the marine intertidal (Lyngbya, Laguna Ojo de Liebre, Mexico), an acidic hotspring (Cyanidium, Nymph Creek, Yellowstone National Park), and an acidic stream at ambient temperature (Zygogonium, Yellowstone National Park). Carbon fixation in the absence of additional CO2 essentially followed the rising and falling sunlight levels, except that during the middle of the day there was a short dip in carbon fixation rates. The addition of CO2 profoundly enhanced carbon fixation rates during the daylight hours, including during the midday dip. Therefore, it is unlikely that the midday dip was due to photoinhibition. Surprisingly, enhancement of carbon fixation was often greatest in the early morning or late afternoon, times when carbon fixation would be most likely to be light limited.     

Stable carbon isotopes: possible clues to early life on Mars.

Organic and inorganic carbon in terrestrial near-surface environments are characterized by a marked difference in their 13C/12C ratios which can be traced back in the Earth's sedimentary record over almost 4 billion years. There is no doubt that the bias in favour of 12C displayed by biogenic matter derives, for the most part, from the isotope-selecting properties of the carbon-fixing enzyme (ribulose-1,5-bisphosphate carboxylase) that is operative in the principal photosynthetic pathway and promotes most of the carbon transfer from the non-living to the living realm. Postulating a universality of biological principles in analogy to the proven universality of the laws of physics and chemistry, we may expect enzymatic reactions in exobiological systems to be beset with B similar kinetic fractionation effects. Hence, the retrieval from the oldest Martian sediments of isotopic fractionations between reduced and oxidized (carbonate) carbon may substantially constrain current conjectures on the possible existence of former life on Mars.     

多元线性回归在引气系统故障诊断中的应用

针对利用快速存取记录器(QAR)数据进行民机系统故障诊断问题,以民机引气系统为对象,提出了一种适合多飞行循环数据特点的多元线性回归模型的故障检测方法.首先建立了多飞行循环数据的引气系统性能多元线性回归模型,设计了飞行循环和飞行循环内故障检测方法;然后采用最大后验估计方法进行模型参数估计;最后设计了适合多飞行循环数据的模型参数最大后验估计算法.借助仿真数据和航空公司收集的实际飞行数据对方法进行了验证,结果表明了该方法有效且具有一定工程应用价值.      

Atmospheric dynamics in the "Laboratory Biosphere" with wheat and sweet potato crops.

Laboratory Biosphere is a 40-m3 closed life system equipped with 12,000 W of high pressure sodium lamps over planting beds with 5.37 m2 of soil. Atmospheric composition changes due to photosynthetic fixation of carbon dioxide and corresponding production of oxygen or the reverse, respiration, are observed in short timeframes, e.g., hourly. To focus on inherent characteristics of the crop as distinct from its area or the volume of the chamber, we report fixation and respiration rates in mmol h-1 m-2 of planted area. An 85-day crop of USU Apogee wheat under a 16-h lighted/8-h dark regime peaked in fixation rate at about 100 mmol h-1 m-2 approximately 24 days after planting. Light intensity was about 840 micromoles m-2 s-1. Dark respiration peaked at about 31 mmol h-1 m-2 at the same time. Thereafter, both fixation and respiration declined toward zero as harvest time approached. A residual soil respiration rate of about 1.9 mmol h-1 m-2 was observed in the dark closed chamber for 100 days after the harvest. A 126-day crop of Tuskegee TU-82-155 sweet potato behaved quite differently. Under a 680 micromoles m-2 s-1, 18-h lighted/6-h dark regime, fixation during lighted hours rose to a plateau ranging from about 27 to 48 mmol h-1 m-2 after 42 days and dark respiration settled into a range of 12-23 mmol h-1 m-2. These rates continued unabated until the harvest at 126 days, suggesting that tuber biomass production might have continued at about the same rate for some time beyond the harvest time that was exercised in this experiment. In both experiments CO2 levels were allowed to range widely from a few hundred to about 3000 ppm, which permitted observation of fixation rates both at varying CO2 concentrations and at each number of days after planting. This enables plotting the fixation rate as a function of both variables. Understanding the atmospheric dynamics of individual crops will be essential for design and atmospheric management of more complex CELSS which integrate the simultaneous growth of several crops as in a sustainable remote life support system.     

Stable isotope analysis at the molecular level: a new approach for determining the origins of amino acids in the Murchison meteorite.

A combined gas chromatography/isotope ratio mass spectrometry (GC/IRMS) method has been developed that permits the direct stable carbon isotope analysis of N(O)-trifluoroacetyl-isopropyl esters of individual amino acids and their respective enantiomers at nanomole abundances. Calculation of the original delta 13C values of the amino acids is accomplished via a correction for the carbon introduced during the derivatization process. Previous GC/IRMS analyses of individual amino acids in the non-hydrolyzed water extract of an interior sample of a Murchison meteorite stone revealed an enrichment in 13C relative to terrestrial organic matter, in agreement with previous findings for bulk extracts. The range of amino acid delta 13C values (+5 to +30%, PDB) suggests possible kinetic effects during synthesis. In this study, an apparent kinetic isotope effect was also observed for the amino acid products of a spark discharge experiment. These preliminary results are supportive of a similar mechanism for the abiotic synthesis of amino acids in the Murchison meteorite.     

The flux of Earth-crossing and moon-cratering interplanetary bodies

Methods of determining the present flux and total number of kilometer-sized Earth-crossing objects are discussed, including (1) probability considerations based on the frequency of chance rediscoveries of the lost objects, (2) evaluation of large-scale photographic surveys for the detection of fast moving objects, and (3) evaluation of close encounters of interplanetary bodies with the Earth. The results are interfaced with the lunar and terrestrial cratering history. It is shown that the discrepancies between these two independent lines of evidence are still within the margin of uncertainty set by observational biases, cratering efficiencies, and surface reflectivities of the objects, in particular as regards extinct cometary nuclei. Impacts of active comets can only be held responsible for a very small fraction of the craters, and impacts of high-albedo Apollo asteroids are consistent with a steady state. There is no definite enhancement of the present-day flux as compared with the average level of cratering during the last 3 Gyr which would require significant variations in the stellar environment of the solar system, affecting the rate of delivery of new comets from the Oort cloud. There is also no evidence of a recent major collisional event in the asteroid belt. Deviations from an equilibrium between source and sink only become effective in the size range of meteor particles, where no long-term cratering record is available. They are apparently due to a very limited number of parent objects, and appear on a time scale which is very short compared with the age of the solar system.     

Biological nitrogen fixation under primordial Martian partial pressures of dinitrogen.

Early Earth and early Mars were similar enough such that past geochemical and climatic conditions on Mars may have also been favorable for the origin of life. However, one of the most striking differences between the two planets was the low partial pressure of dinitrogen (pN2) on early Mars (18 mb). On Earth, nitrogen is a key biological element and in many ecosystems the low availability of fixed nitrogen compounds is the main factor limiting growth. Biological fixation of dinitrogen on Earth is a crucial source of fixed nitrogen. Could the low availability of dinitrogen in the primordial Martian atmosphere have prevented the existence, or evolution of Martian microbiota? Azotobacter vinelandii and Azomonas agilis were grown in nitrogen free synthetic medium under various partial pressures of dinitrogen ranging from 780-0 mb (total atmosphere=1 bar). Below 400 mb the biomass, cell number, and growth rate decreased with decreasing pN2. Both microorganisms were capable of growth at a pN2 as low as 5 mb, but no growth was observed at a pN2 < or = 1 mb. The data appear to indicate that biological nitrogen fixation could have occurred on primordial Mars (pN2=18 mb) making it possible for a biotic system to have played a role in the Martian nitrogen cycle. It is possible that nitrogen may have played a key role in the early evolution of life on Mars, and that later a lack of available nitrogen on that planet (currently, pN2=0.2 mb) may have been involved in its subsequent extinction.     

Solar spectral irradiance variability in the ultraviolet from SORCE and UARS SOLSTICE

The SOLar-STellar Irradiance Comparison Experiment (SOLSTICE) on the SOlar Radiation and Climate Experiment (SORCE) has been measuring the solar spectral irradiance on a daily basis since early 2003. This time period includes near-solar maximum conditions, the Halloween storms of 2003, and solar minimum conditions. These results can be compared to observations from the SOLSTICE I experiment that flew on the Upper Atmosphere Research Satellite (UARS) during the decline of the previous solar cycle as well as with currently operating missions. We will discuss similarities and differences between the two solar cycles in the long-term ultraviolet irradiance record.     

Solar radiation variability in the last 1400 years recorded in the carbon isotope ratio of a mediterranean sea core

We present measurements and data analysis of the carbon stable isotopes (δ¹³C) in the planktonic Globigerinoides ruber extracted from the GT90/3 shallow water Ionian sea core, dated with high precision. It is commonly accepted that δ¹³C variations in symbiontic foraminifera mainly record the effects of productivity and of photosynthetic activity, varying with the ambient light level. Therefore from this time series we can deduce information on the sea surface illumination at the time of the planktonic foraminifera growth. The profile (359 points) covers the period 590–1979 AD, with a resolution of 3.87 years and it is an extension of the time series (215 points) previously published in this journal. The spectral analysis of the longer time series confirms the presence of the 11 y signal, with amplitude 0.08‰ (peak-to-trough), found in the shorter time series in phase with the sunspot solar cycle; furthermore it shows the presence of two centennial cycles of 100 and 200 years, with amplitude 0.08‰ and 0.02‰ respectively. These components are identified at high significance level by Monte Carlo singular spectrum analysis (MC-SSA). A comparison between the δ¹³C profile and the historical aurorae series (600–1500 AD) shows that the long-term δ¹³C variations are at least partially generated by the solar activity modulation and in phase with the solar output, as represented by the solar wind interaction with the magnetosphere.     

低速冲击下碳/玻混杂复合材料红外辐射特征

碳/玻混杂复合材料在工业应用中表现出巨大的应用潜力。基于红外热像法试验研究了碳/玻混杂复合材料层压板与2种非混杂材料低速冲击下的红外辐射特征。通过目视、超声C扫描和光学显微镜等方法确定冲击后层压板的损伤模式,分析热图序列的时序变化特征和温度分布特征,从而表征冲击过程中的热耗散效应。结果表明：红外热成像技术非常适合监测低速冲击下纤维增强复合材料的损伤过程,通过热图序列可以建立起监测特征与各损伤模式之间的联系;同时发现碳/玻纤维的层间混杂可有效提升碳纤维强基复合材料（CFRP）的抗分层能力,随着冲击能量增加其抗分层能力愈加明显,冲击后的碳/玻混杂复合材料兼具较大的表面损伤和较小的分层损伤,拥有较好的损伤容限。     

Solar cycle variation of the low-altitude trapped proton flux

Under NASA's Space Environment Effects (SEE) program, we are developing new models for the low-altitude (250–1000 km, L < 1.5) trapped radiation environment based on data from the TIROS/NOAA polar orbiting spacecraft. The unique features of this data base and model include the long time series (more than one complete solar cycle) obtained from the TIROS/NOAA data and the use of a coordinate system more applicable to the low-altitude environment. The data show a strong variation (as much as a factor of 10) over the solar cycle and a hysteresis effect between the rising and falling portions of the solar cycle. Both the solar cycle variation and the hysteresis are functions of L. In addition to the hysteresis effect, the flux during a given cycle appears to be a function of the previous cycle. Superimposed on the gradual variation over the solar cycle, transient effects, correlated with solar particle events (SPEs), can be clearly seen. Comparison with the AP8 models shows that the measured flux is a factor of 2–3 higher than the model. These data have important implications for the development and use of trapped radiation models, and will also contribute to our knowledge of the source and loss mechanisms at work in the inner zone.     

Solar activity variations of equatorial spread F occurrence and sustenance during different seasons over Indian longitudes: Empirical model and causative mechanisms

A comprehensive analysis using nearly two decades of ionosonde data is carried out on the seasonal and solar cycle variations of Equatorial Spread F (ESF) irregularities over magnetic equatorial location Trivandrum (8.5°N, 77°E). The corresponding Rayleigh Taylor (RT) instability growth rates (γ) are also estimated. A seasonal pattern of ESF occurrence and the corresponding γ is established for low solar (LSA), medium solar (MSA) and high solar (HSA) activity periods. For LSA, it is seen that the γ maximizes during post sunset time with comparable magnitudes for autumnal equinox (AE), vernal equinox (VE) and winter solstice (WS), while for summer solstice (SS) it maximizes in the post-midnight period. Concurrent responses are seen in the ESF occurrence pattern. For MSA, γ maximizes during post-sunset for VE followed by WS and AE while SS maximises during post-midnight period. The ESF occurrence for MSA is highest for VE (80%), followed by AE (70%), WS (60%) and SS (50%). In case of HSA, maximum γ occurs for VE followed by AE, WS and SS. The concurrent ESF occurrence maximizes for VE and AE (90%), WS and SS at 70%.The solar cycle variation of γ is examined. γ shows a linear variation with F10.7?cm flux. Further, ESF percentage occurrence and duration show an exponential and linear variation respectively with γ. An empirical model on the solar activity dependence of ESF occurrence and sustenance time over Indian longitudes is arrived at using the database spanning two solar cycles for the first time.     

The seeding of life by comets.

The evidence that living organisms were already extant on the earth almost 4 Gyr ago and that early bombardment by comets and asteroids created a hostile environment up to about this time has revived the question of how it was possible for prebiotic chemical evolution to have provided the necessary ingredients for life to have developed in the short intervening time. The actual bracketed available temporal space is no more than 0.5 Gyr and probably much less. Was this sufficient time for an earth-based source of the first simple organic precursor molecules to have led to the level of the prokaryotic cell? If not, then the difficulty would be resolved if the ancient earth was impregnated by organic molecular seed from outer space. Curiously, it seems that the most likely source of such seeds was the same a one of the sources of the hostile enviroment, namely the comets which bombarded the earth. With the knowledge of comets gained by the space missions it has become clear that a very large fraction of the chemical composition of comet nuclei consists of quite complex organic molecules. Furthermore it has been demonstrated that comets consist of very fluffy aggregates of interstellar dust whose chemistry derives from photoprocessing of simple ice mixtures in space. Thus, the ultimate source of organics in comets comes from the chemical evolution of interstellar dust. An important and critical justification for assuming that interstellar dust is the ultimate source of prebiotic molecular insertion on the earth is the proof that comets are extremely fluffy aggregates, which have the possibility of breaking up into finely divided fragments when the comet impacts the earth's atmosphere. In the following we will summarize the properties of interstellar dust and the chemical and morphological structure of comets indicated by the most recent interpretations of comet observations. It will be shown that the suitable condition for comets having provided abundant prebiotic molecules as well as the water in which they could have further evolved are consistent with theories of the early earth environment.     

A study of the solar cycle signal on total ozone over low-latitude Brazilian observation stations

Observations of total ozone at low latitudes in Brazil have been made using Dobson spectrophotometers since 1974 for Cachoeira Paulista (23.1° S, 45° W) and since 1978 for Natal (5.8° S, 35.2° W). Annual averages, 12 months and 36 months running averages have been analyzed. Spectral analyses of the data revealed that the most important periods found (confidence level> 90%) were: for Natal, 2.5 years (93.1%, quasi-biennial oscillation-QBO) and 10 years (98,2%, possibly the solar cycle signal); for Cachoeira Paulista, 2.4 years (96.8%, QBO) and 8 years (99.6%). The difference in total ozone between maximum and minimum solar cycles were estimated, using yearly averages of total ozone. For solar cycle 21, 1.16% and 1.26% for Natal and Cachoeira Paulista were found; for solar cycle 22, a larger difference of 3.8% for Natal and 4.1% for Cachoeira Paulista were found. The corresponding variation in UV-B at 300 nm, using Beer's law, is 8–10% for C. Paulista and 4–5% for Natal, with maxima occurring during the minimum of the solar cycle.     

Mapping hydrothermal alteration zones and lineaments associated with orogenic gold mineralization using ASTER data: A case study from the Sanandaj-Sirjan Zone,Iran

The Sanandaj-Sirjan Zone (SSZ) is considered as an important region for gold exploration in the western sector of Iran. Its mountainous topography and unpaved routes make its study challenging for researchers and raise the costs for mining companies strating new exploration plans. Gold mineralization mainly occurs as irregular to lenticular sulfide-bearing quartz veins along shear zones in deformed mafic to intermediate metavolcanic and metasedimentary rocks. In this investigation, ASTER data are used for mapping hydrothermal alteration minerals and to better discriminate geological structural features associated with orogenic gold occurrences in the area. Image transformation techniques such as specialized band ratioing and Principal Component Analysis are used to delineate lithological units and alteration minerals. Supervised classification techniques, namely Spectral Angle Mapper (SAM) and Spectral Information Divergence (SID) are applied to detect subtle differences between indicator alteration minerals associated with ground-truth gold locations in the area. The directional filtering technique is applied to help in tracing along the strike the different linear structures. Results demonstrate that the integration of image transformation techniques and supervised classification of ASTER data with fieldwork and geochemical exploration studies has a great efficiency in targeting new prospects of gold mineralization in the SSZ. The approach used in this research provides a fast, cost-efficient means to start a comprehensive geological and geochemical exploration programs in the study area and elsewhere in similar regions.