Cellular responses to gravity: extracellular, intracellular and in-between.

Our understanding of gravitational effects (inertial effects in the vicinity of 1 x g) on cells has matured to a stage at which it is possible to define, on the basis of experimental evidence, extracellular effects on small cells and intracellular effects on eukaryotic gravisensing cells. Yet undetermined is the nature of response, if any, of those classes of cells that are not governed solely by extracellular physical events (as are prokaryotes) and are devoid of obvious mechanical devices for sensing inertial forces (such as those possessed by certain plant cells and sensory cells of animals). This "in-between" class of cells needs to be understood on the basis of the combination of intracellular and extracellular gravity-dependent processes that govern experimentally-measurable variables that are relevant to the cell's responses to modified inertial forces. The forces that certain cell types generate or respond to are therefore compared to those imposed by approximately 1 x g in the context of cytoskeletal action and symmetry-breaking pathways.     

Boundary-layer analysis for the convection/diffusion transition in dendritic growth

Earlier ground-based studies have shown that a sharp transition occurs in the dominant mode of heat transport during dendritic solidifcation. Specifically, convective heat transport dominates below the transition supercooling and diffusive heat transport dominates above. Thus the influence of gravitational acceleration on dendritic crystallization depends critically on the overall thermodynamic driving force. Since many practical solidification processes occur at relatively small supercoolings, understanding the nature of the transition in transport mode is essential for both terrestrial and space processing. A boundary-layer analysis has been carried out wherein the diffusion boundary-layer thickness (Stefan length) is compared with the thermal boundary layer thickness determined from the fluid mechanics of natural convection. Our analysis shows that the Stefan length δ_S decreases rapidly with supercooling (ΔT^?2.5) whereas the thermal boundary layer thickness δ_T decreases slowly (ΔT^?.25). The transition supercooling at which the transport mode switches from convective to diffusive occurs where δ_T = δ_S. The influence of gravitational acceleration, and the thermo-physical properties of the molten phase will be discussed, along with avenues for conducting future research.     

High phosphorylase activity is correlated with increased potato minituber formation and starch content during extended clinorotation.

The major purpose of these experiments were to investigate growth of potato storage organs and starch synthesis in minitubers at slow horizontal clinorotation (2 rpm), which partly mimics microgravity, and a secondary goal was to study the activity and localization of phosphorylase (EC 2.4.1.1) in storage parenchyma under these conditions. Miniplants of Solanum tuberosum L. (cv Adreta) were grown in culture for 30 days for both the vertical control and the horizontal clinorotation. During long-term clinorotation, an acceleration of minituber formation, and an increase of amyloplast number and size in storage parenchyma cells, as well as increased starch content, was observed in the minitubers. The differences among cytochemical reaction intensity, activity of phosphorylase, and carbohydrate content in storage parenchyma cells of minitubers grown in a horizontal clinostat were established by electron-cytochemical and biochemical methods. It is shown that high phosphorylase activity is correlated with increased starch content during extended clinorotation. The results demonstrate the increase in carbohydrate metabolism and possible accelerated growth of storage organs under the influence of microgravity, as mimicked by clinorotation; therefore, clinorotation can be used as a basis for future studies on mechanisms of starch synthesis under microgravity.     

Energetic metabolism response in algae and higher plant species from simulation experiments with the clinostat.

Adenylate state is acknowledged to be among the most convenient approaches in the study of physiological changes in plant cells under simulation of altered gravity condition with the clinostat. Adenylate levels and the ATP/ADP ratio in cytoplasmic and mitochondrial extracts of cultivated cells of Haplopappus gracilis and algae cells of Chlorella vulgaris under initial stages of the fast-rotating and slow-rotating clinorotation, as well as the long-term clinorotation, have been investigated. For analysis of ATP and ADP levels in the plant cells under the clinorotation, we applied a high-sensitive bioluminescence method using the luciferase and piruvate kinase enzyme systems. It has been shown that the adenylate ratio is already increased during at the start of clinorotation with the different speed of rotation in the biological material tested. The considerable changes in mitochondrial ultrastructure of Chlorella cells, as well as the rising ATP level and dropping of the ATP/ADP ratio appear after long-duration clinorotation if compared to control material. It is probably connected with the distinctions in ATP-synthetase functioning in mitochondria of the cells under the clinorotation conditions.     

Travelling heater growth of GaSb under reduced gravity during the first spacelab-mission

Single crystals of binary III-V-semiconductors, e.g. GaAs or InP, are important basic materials for optoelectronic devices, e.g. LED's and lasers. Device production needs highly perfect substrate crystals with low defect densities and homogeneous dopant distributions. In our experiment we applied the Travelling-Heater-Method to grow the III-V compound GaSb. The aim of this research project was to improve the crystal quality by investigating convective transport phenomena and the origins of dopant inhomogeneities under earth and space conditions. Earth grown crystals show strong dopant variations mainly due to convective flow phenomena. The preliminary result of our SPACELAB 1 experiment reveals an increase of dopant homogeneity in the space grown crystal because of the absence of natural convection under reduced gravity.     

基于DFT的水射流红外热像频域时空分析

基于二维离散傅里叶变换及空间频谱分析,对水射流湍流脉动的空间尺度进行了研究,得到了由红外辐射温度表征的被动标量湍流场在对流区、耗散区、惯性子区的特征空间尺度及其时间演化规律.对射流不同区域的关心点重新采样,得到湍流场中关心点的时间序列,利用一维离散傅里叶变换,分析了对流区大尺度涡中心、惯性子区小尺度涡中心、耗散区及射流轴心线上各关心点的湍流波动特征.计算了时间序列频谱的分形维数,研究了自由湍流不同尺度区间上述各关心点湍流脉动的分形特征.      

Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation.

Here, we report changes gene expression and morphology of the renal epithelial cell line, A6, which was derived from Xenopus laevis adult kidney that had been induced by long-term culturing with a three-dimensional clinostat. An oligo microarray analysis on the A6 cells showed that mRNA levels for 52 out of 8091 genes were significantly altered in response to clinorotation. On day 5, there was no dramatic change in expression level, but by day 8 and day 10, either upregulation or downregulation of gene expression became evident. By day 15, the expression levels of 18 out of 52 genes had returned to the original levels, while the remaining 34 genes maintained the altered levels of expression. Quantitative analyses of gene expression by real-time PCR confirmed that changes in the mRNA levels of selected genes were found only under clinorotation and not under hypergravity (7 g) or ground control. Morphological changes including loss of dome-like structures and disorganization of both E-cadherin adherence junctions and cortical actin were also observed after 10 days of culturing with clinorotation. These results revealed that the expression of selected genes was altered specifically in A6 cells cultured under clinorotation.     

EFFECTS OF CONVECTIVE FLUID MOTION UPON OXIDE CRYSTAL GROWTH IN HIGH TEMPERATURE SOLUTION

For understanding of the influence of convective flow on crystal growth, space high temperature in situ observation instrument (SHITISOI) is dedicated to visualize and record the whole growth process of oxide crystals in high temperature up to 1000°C. Model experiments using transparent liquids such as KNbO3 and a mix ture of Li2B4O7+KNbO3 were chosen to investigate effects on ground and in space.On the earth, an investigation of growth kinetics of KNbO3 crystal related to two different states of convection: diffusive-advective flow and diffusive-convective flow,has been performed. The per unit length of a step e is calculated from the exper imental data for two different states of convection. Analyses of these data show the effect of buoyancy convection is to enhance the sharpness of the interface. The growth of KNbO3 crystals from solution of KNbO3+Li2B4O7 was investigated in space. The streamlines of the steady thermocapillary convection in Li2B4O7 solvent was observed. Due to thermocapillary convection, KNbO3 crystal grains grew and filled the whole solution homogeneously. Earth-based quenching experiments are de signed in order to study polyhedral instability of KNbO3 crystal, which is controlled by diffusion mechanism limitation. In all cases, when the crystal was nucleated near air/solution surface, it lost its polyhedral stability and varied from polyhedrons to dedrites. The thickness of diffusion mechanism limitation layer is about 60μm.     

Calcium balance in pea root statocytes under both clinorotation and Ca2+ channel blockers' influence.

We have previously demonstrated that space flight and clinorotation conditions increase cytoplasmic Ca2+ level in pea root statocytes. A rise in [Ca2+]i may be a serious problem for plants in microgravity environment. It is hypothesized that involvement of Ca2+ channel blockers in the growth medium may rescue a plant from abundance of Ca2+ ions. Indeed, combination of clinorotation (2 rpm, 5 days) and any Ca2+ channel blocker (1 micromole D600 or nicardipine, 12 hr) causes decreasing the Ca2+ concentration in pea root statocytes in comparison with clinorotation alone. Redistribution of Ca(2+)-ATPase activities observed under clinorotation comes to normal after D600 application whereas following by nicardipine action the pattern of the cytochemical staining is intermediate between those in stationary control and under clinorotation. Our data support the hypothesis that Ca2+ channel blockers may act as protectors for plants against rise in [Ca2+]i. The role for Ca2+ channels in graviperception and in microgravity effects as well as ways for stabilization of Ca2+ balance in plant cells in space flights are discussed.     

Solidification of GeSi solid solution in near-zero-G conditions

In summary, it should be stated that the existing theories of segregation during directional crystallization which consider mass transport in melt due only to either concentration diffusion or convective stirring (or both) cannot account for solute segregation picture observed in solid solution crystals which have been obtained aboard Apollo-Soyuz orbital complex. To do this, some other, new models are required.     

IMF effect on ionospheric trough occurrence at equinoxes

Previous observations have shown that there is a relationship between the F region trough and both B_z and B_y components of the interplanetary magnetic field (IMF). Since IMF governs the polar cap convection, we investigate here if this relationship can be explained by means of polar cap convection. The study is limited to equinox seasons. The poleward and equatorward edges of the trough are determined from satellite tomographic observations and their locations are plotted in magnetic coordinates together with the convection pattern given by Papitashvili and Rich [Papitashvili, V.O., Rich, F.J. High-latitude ionospheric convection models derived from DMSP ion drift observations and parameterized by the IMF strength and direction. J. Geophys. Res. 107, 2002, doi:10.1029/2001JA000264] using IMF measurements coincident with trough observations. The results indicate a close relationship between the troughs and convection. Most of the troughs are seen within the dusk cell and the pattern of trough observations rotates with the convection pattern, when B_y changes its sign. More dayside troughs are observed when B_z is negative than in the opposite case, i.e. fast convective flow favours the dayside trough occurrence. Nightside troughs are observed more frequently when B_y is negative. In both evening and morning sectors the trough is situated close to the edges of convection cells, which partly contradicts previous results showing that the troughs are associated with the convection reversal. It is concluded that plasma convection has an important role in trough generation, although the effect of a strong electric field and other mechanisms like precipitation certainly have a role of their own.     

Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

Modeling the radiation belts: what are the important physical processes to be taken into account in models?

During active periods, physical processes acting on particle dynamics are well known to have several origins and can play a role at different times, different locations, and with different time scales. As their effects can be opposite, it is necessary to identify them and their relative importance:

• the particle sources (plasmasheet or direct solar wind entry)

• the particle losses (precipitation or drift loss)

• the particle transport and acceleration (convection access, magnetic or electric pulse or variation and recirculation).

The various phenomena are explained and results are presented. In particular, we demonstrate that classical diffusion models like the Salammbo code can account for all these phenomena.     

Solar wind-Venus interaction affecting ionospheric electron density profile

The equilibrium electron density profile has been computed and compared with measured profiles by Venera 9 and Mariner 5 and 10. The computed electron density profile is seen to show discrepancies with measured data. The contribution of solar wind interaction induced convection to equilibrium electron density profile has been estimated. It is found that the convective processes are less important at lower altitudes, whereas at higher altitudes its contribution becomes dominant. The night side Venus ionosphere is formed due to the transport of O⁺ and impact ionization of neutral gases by suprathermal electrons. The discrepancies in theoretical and measured electron density profiles provide clear indication of additional energy source of solar wind origin.     

Global tectonics and metallogeny: Deep roots of some ore-controlling fracture zones. A possible relation to small-scale convective cells at the base of the lithosphere?

Criteria suggest that some of the ore-controlling fracture zones have deep roots, some extending into the upper mantle. The question is analyzed of whether a pattern of deep-seated fracture zones, extending to the base of a continental lithosphere, may be related to, or interact with, the small-scale convective cells (rolls) which originate [1,2] in the mantle beneath a moving lithospheric plate.The analysis follows a previous application of the same concept by others [3,4] who explained the origin of a chain of volcanoes by the movement of an oceanic lithospheric plate over a pattern of small-scale convective cells of the underlying mantle.The Canadian Shield is used to discuss the above question. A correlation has been found between: (1) the direction of two sets of long wavelength gravity anomalies detected by Stephenson and Beaumont, [8] in a study of the isostatic response of the Canadian Shield, and (2) the pattern of N-S and E-W trending trajectories of ore-controlling fracture zones, postulated by the author [5,6] in the southern part of the Canadian Shield. The Hudson Bay Paleolineament [7] correlates very well with the N-S set of gravity anomalies.The above correlation suggests that the ore-controlling lineaments and the long wavelength anomalies of the Canadian Shield are related. If the gravity anomalies reflect, as Stephenson and Beaumont [8] tentatively suggest, a pattern of convective cells beneath the base of the lithosphere, then the volcanic activity and metallogenesis may be related to the boundaries and corners of the cells. The author suggests that the convective cells could, in this case, originate by melting of the mantle material proceeding preferentially along the intersecting deep-seated fracture zones.The pattern of deep-seated fracture zones, compiled for the western United States [9] also shows a relationship of major ore deposits and ore clusters to the corners of rectangular blocks, defined by mutual intersection of the E-W and N-S fracture zones. In this case, the size of the blocks, measured in an E-W direction, is about 530 km, and in a N-S direction about 600 km. These figures are significantly close to the distance from the seismic discontinuity at a depth of 650 km, which is considered by others [2,4] as the lower boundary of the small-scale convection.     

Patterns of cortical microtubules formed in epidermis of Beta vulgaris L. roots under clinorotation.

Changes of cortical microtubules (MTs) from the normal transverse arrangement were observed in epidermal cells of Beta vulgaris roots under clinorotation. We hypothesize that the epidermis is sensitive to clinorotation and that the microtubular cytoskeleton plays a key role in the ensuing growth response.     

蒸发效应与热毛细对流耦合现象的实验研究

实验研究了矩形液池中蒸发薄液居中蒸发效应与热毛细对流的耦合机理. 对于单纯的热毛细对流稳定性从实验和理论上已有深入研究,但目前国际上对带有菇发界面的热毛细对流问题尚缺乏研究. 特别是近来的研究发现,气液界面的蒸发对热毛细对流稳定性有很大的影响. 本实验以温度为主要控制参数,测量了不同工况下蒸发界面不同点的蒸发速率和表层温度,并利用 PIV 方法分析得到了液体内的嘛场分布. 实验结果发现,随着沿界面的温差增加,蒸发液体内的流型从稳定的单涡胞结构变为稳定的多祸胞结构,并最终演变为紊流结构. 综合分析以上测量结果并与理论分析结果进行了比较。      

Gravity, cellular membranes and associated processes: an introduction.

The aim of the session "Gravity: Cellular Membranes and Associated Processes" was to bring together scientists from different biological disciplines concentrated on the same scientific question: What are the basic interactions or influences, respectively, of gravity on cellular, molecular level? Presentations were selected dealing with the interaction of gravity with basic physico-chemical processes of membranes, such as changes of the membrane composition of human erythrocytes up to ultrastructural changes in a fungus and other objects after exposure to the conditions of space flight, clinorotation or increased acceleration by means of centrifugation. Taken together the data presented here and in the other session of the symposium on "Life and Gravity", clearly outline that future basic physico-chemical studies, and studies dealing with the molecular basis of the cellular signal-perception and transduction, have to be pressed forward in order to understand signal-responses on cellular level, but also of a whole organism.     

太阳大气中非线性波的数字计算

本文用多方过程方程式封闭一维带引力场的流体力学方程组,用不同的γ值计算了不同类型的扰动在HSRA太阳大气模型中向上传播和演化。说明在太阳大气中向上传播的小扰动能够发展成为激波;激波的形成高度基本上不受热力学过程的影响,而与扰动强度关系很大,愈小的扰动传播愈高;但由于太阳大气中湍流场的存在,完全有可能使这样小的扰动在未形成激波前就被破坏掉。这就证实了一些人认为的对流层机械波并不是一个对色球和日冕的有效加热机制的说法。