Histochemical localisation of carbonic anhydrase in the inner ear of developing cichlid fish,Oreochromis mossambicus

Inner ear otolith growth in terms of mineralisation mainly depends on the enzyme carbonic anhydrase (CAH). CAH is located in specialised, mitochondria-rich macular cells (ionocytes), which are involved in the endolymphatic ion exchange, and the enzyme is responsible for the provision of the pH-value necessary for otolithic calcium carbonate deposition.     

Effect of hypergravity on carboanhydrase reactivity in inner ear ionocytes of developing cichlid fish.

It has been shown earlier that hypergravity slows down inner ear otolith growth in developing fish. Otolith growth in terms of mineralization mainly depends on the enzyme carboanhydrase (CA), which is responsible for the provision of the pH-value necessary for calcium carbonate deposition. Larval siblings of cichlid fish (Oreochromis mossambicus) were subjected to hypergravity (3 g, hg; 6 h) during development and separated into normally and kinetotically swimming individuals following the transfer to 1 g (i.e., stopping the centrifuge; kinetotically behaving fish performed spinning movements). Subsequently, CA was histochemically demonstrated in inner ear ionocytes (cells involved in the endolymphatic ion exchange) and enzyme reactivity was determined densitometrically. It was found that both the total macular CA-reactivity as well as the difference in reactivities between the left and the right maculae (asymmetry) were significantly lower (1) in experimental animals as compared to the 1 g controls and (2) in normally swimming hg-animals as compared to the kinetotically behaving hg-fish. The results are in complete agreement with earlier studies, according to which hypergravity induces a decrease of otolith growth and the otolithic calcium incorporation (visualized using the calcium-tracer alizarin complexone) of kinetotically swimming hg-fish was higher as compared to normally behaving hyper-g animals. The present study thus strongly supports the concept that a regulatory mechanism, which adjusts otolith size and asymmetry as well as otolithic calcium carbonate incorporation towards the gravity vector, acts via activation/deactivation of macular CA.     

The magnetic fields of Mercury,Venus and Mars

Just as clearly as Mariner 10 established that Mercury has an intrinsic magnetic field, the Pioneer Venus orbiter has established that Venus has no significant intrinsic field. This is perhaps the opposite of what might be expected. Mercury, a small planet might be expected to cool rapidly and its internal dynamo to cease, while Venus, which is almost as large as the Earth, should not have lost much heat. On the contrary the source of energy of the Mercury dynamo appears to be extant whereas that of Venus appears to be extinct.The existence of a Martian magnetic field is controversial. No unambiguous signature of a Martian magnetic field has been reported. If the field on the nightside of Mars is of planetary rather than solar origin the Russian Mars spacecraft observations indicate the Martian dipole lies near the planetary equator rather than its pole.     

A review of solar proton events during the 22nd solar cycle.

Solar cycle 22 had significant, large fluence, energetic particle events on a scale reminiscent of the 19th solar cycle. Examination of the characteristics of these large events suggests that some of the old concepts of spectral form, intensity-time envelope and energy extrapolations, used to estimate the dose from large events that occurred during previous solar cycles should be re-evaluated. There has also been a dramatic change in perspective regarding the source of solar protons observed in interplanetary space. Very large fluence events are associated with powerful fast interplanetary shocks. The elemental composition and charge state of these events is suggestive of a dominate source in the solar corona and not from a very hot plasma. Furthermore, there is a strong suggestion that the intensity-time profile observed in space is dominated by the connection of the observer to an interplanetary shock source rather than to a unique location near the surface of the sun. These concepts will be examined from the perspective of energetic particles contributing to the dose experienced by an astronaut on an interplanetary space mission.     

Structural and functional organisation of regenerated plant protoplasts exposed to microgravity on Biokosmos 9.

Preparatory experiments for the IML-1 mission using plant protoplasts, were flown on a 14-day flight on Biokosmos 9 in September 1989. Thirty-six hours before launch of the biosatellite, protoplasts were isolated from hypocotyl cells of rapeseed (Brassica napus) and suspension cultures of carrot (Daucus carota). Ultrastructural and fluorescence analysis of cell aggregates from these protoplasts, cultured under microgravity conditions, have been performed. In the flight samples as well as in the ground controls, a portion of the total number of protoplasts regenerated cell walls. The processes of cell differentiation and proliferation under micro-g did not differ significantly from those under normal gravity conditions. However, in micro-g differences were observed in the ultrastructure of some organelles such as plastids and mitochondria. There was also an increase in the frequency of the occurrence of folds formed by the plasmalemma together with an increase in the degree of complexity of these folds. In cell cultures developed under micro-g conditions, the calcium content tends to decrease, compared to the ground control. Different aspects of using isolated protoplasts for clarifying the mechanisms of biological effects of microgravity are discussed.     

Gravitational response of the slime mold Physarum.

The acellular slime mold Physarum polycephalum is used as a model system to investigate the graviresponse of single cells which possess no receptors specialized for the perception of gravity. To obtain insights into the gravity-signal transduction mechanism the light response of the cell is used: Macroplasmodia of the slime mold show clear geo- and phototaxes. Gravity increases and white light decreases transiently the contraction frequency of plasmodial strands whereby both responses follow the same time pattern. Since mitochondria play a major role in changing the contraction rhythm in response to light and gravity stimuli, the simultaneous and subsequent inductions of the opposing light and gravity responses and their mutual influences on one another were investigated. The experiments were performed in weightlessness (0 g)--simulated on the fast-rotating clinostat as well as in actual weightlessness during the IML-1 Space Shuttle mission. The results indicate that mitochondria (chondriome) are part of the acceleration-stimulus reaction chain in Physarum. Two models for a direct gravireceptor mechanism are discussed.     

Potential sites for the perception of gravity in the acellular slime mold Physarum polycephalum.

Recently a gravisensitivity of the acellular slime mold Physarum polycephalum, which possesses no specialized gravireceptor, could be established by conducting experiments under simulated and under real near weightlessness. In these experiments macroplasmodia showed a modulation of their contraction rhythm followed by regulation phenomena. Until now the perception mechanism for the gravistimulus is unknown, but several findings indicate the involvement of mitochondria: A) During the impediment of respiration the 0g-reaction is inhibited and the regulation is reduced. B) The response to a light stimulus and the following regulation phenomena strongly resemble the behavior during exposure to 0g, the only difference is that the two reactions are directed into opposite directions. In the blue-light reaction a flavin of the mitochondrial matrix seems to be involved in the light perception. C) The contraction rhythm as well as its modulations are coupled to rhythmic changes in the levels of ATP and calcium ions, involving the mitochondria as sites of energy production and of Ca(++)-storage. So the mitochondria could be the site of the regulation and they possibly are the receptor sites for the light and gravity stimuli. Also the observation of a morphologic polarity of the slime mold's plasmodial strands has to be considered: Cross-sections reveal that the ectoplasmic wall surrounding the streaming endoplasm is much thinner on the physically lower side than on the upper side of the strand--this applies to strands lying on or hanging on a horizontal surface. So, in addition to the mitochondria, also the morphologic polarity may be involved in the perception mechanism of the observed gravisensitivity and of the recently established geotaxis. The potential role of the nuclei and of the contractile elements in the perception of gravity is also discussed.     

Do we know the cosmic-ray boosters?

The literature on cosmic-ray acceleration tends to favour strong shocks (in supernovae, stellar and galactic winds) over compact boosters. Difficulties are posed by the spectrum which does not reveal the implied upper cutoff (at E = 10^13±1 eV), by the energy-dependent anisotropy at the high-energy end (at E 10¹⁸ eV), the missing hydrogen, the large required boosting efficiency, the high positron abundance at E 10 GeV, detailed studies of supernova shells, and by an inadequacy of the Sedov-Taylor model for supernova explosions. Solar and solar-wind data support single-step or few-step accelerations because of the large observed anisotropies and short time scales.

The importance of spindown in compact binary systems is often underestimated. Several neutron-star binaries have been detected at cosmic γ-ray energies. Cosmic-ray escape from the Galaxy may proceed supersonically through narrow tubes rather than via convective diffusion.     

Neural [correction of Neutral] networks for control, identification and diagnosis.

Advances in the theory and technology of artificial neural networks provide the potential for new approaches to the problems of control, identification, and diagnosis for large, complex systems. However, these approaches must be validated for specific applications before they can be exploited effectively. Because of the unique capabilities they offer, neural networks should play an important role in space exploration systems operations. After a brief introduction to neural networks is presented, some applications of neural networks to identification and control of space systems are described and discussed. They span the spectrum of relatively straightforward to rather complex applications. An explanation of how neural networks can be applied to such important tasks as fault diagnosis and accommodation is presented. Neural networks are shown to be part of the hierarchy of intelligent control where a higher order decision element monitors and supervises lower order elements for sensing and actuation.     

Comparative characteristic of mitochondria ultrastructural organization in Chlorella cells under altered gravity conditions.

Results from experiments that used cells from the unicellular alga Chlorella vulgaris (strain Larg-1) grown on a clinostat, demonstrated the occurrence of rearrangements in cellular organelles, including changes in the mitochondrial ultrastructure compared to controls. Changes in mitochondrial structure were observed in auto- and heterotrophic regimes of cells grown in altered gravity conditions, especially in long-term experiments. The mitochondrial rearrangements become apparent during cell proliferation, which resulted in an increase in the relative volume of mitochondria per cell: up to 2.7 +/- 0.3% in short-term clino-rotation (2.2 +/- 0.1% in the control) and up to 5.3 +/- 0.4% and 5.1 +/- 0.4% in long-term clinorotation (2.3 +/- 0.2% in the control). The size of the mitochondria and their cristae increased in cells grown under long-time clinorotation. In addition, hypertrophied organelles, not typical for this strain, were observed. These changes in the cells were accompanied by increased electron density of the matrix and a well-ordered topography of the cristae. To examine the separation of oxidative phosphorylation and respiration, an inhibitory agent 2,4-dinitrophenol (2,4-DNP) was applied to cells which resulted in insignificant volume changes of the mitochondria (2.5 +/- 0.4% versus 2.1 +/- 0.2% in the control). The increase of mitochondrial size with regularly arranged cristae, with more condensed matrix and extension of cristae areas of clino-rotated cells, may demonstrate higher functional activity of the mitochondria under altered gravity conditions. Changes observed early in clinorotated cells, in particular the increased level of respiration, adenylate content (especially ATP) and more intensive electron-cytochemical reactions of Mg2(+)-ATPase and succinate [correction of succinat] dehydrogenase (SDH) in mitochondria (including hypertrophic organelles), also suggest increased activity of mitochondria from cells grown under altered gravity conditions compared to controls.     

The amphibian egg as a model system for analyzing gravity effects.

Amphibian eggs provide several advantageous features as a model system for analyzing the effects of gravity on single cells. Those features include large size, readily tracked intracellular inclusions, and ease of experimental manipulation. Employing novel gravity orientation as a tool, a substantial data base is being developed. That information is being used to construct a 3-D model of the frog (Xenopus laevis) egg. Internal cytoplasmic organization (rather than surface features) are being emphasized. Several cytoplasmic compartments (domains) have been elucidated, and their behavior in inverted eggs monitored. They have been incorporated into the model, and serve as a point of departure for further inquiry and speculation.     

Local stability analysis of interface region of astrophysical viscous shear flows with a gradual velocity gradient

In this paper, we extend the stability analysis of cold sharp shear flows to warm astrophysical cases with, inevitable, gradual velocity gradient in the interface region in the presence of viscosity effect. Using linear perturbation theory as well as the local approximation method, the instability growth rate of the excited electromagnetic modes has been investigated for the relativistic and non-relativistic cases of solar wind interacting with interstellar plasma medium. Results show that astrophysical shear systems with a small velocity gradient in the transition region are more stable rather than larger ones. Moreover, dependent on the viscosity coefficient value, the viscosity effects could have a positive role on the instability growth rate of the system in some range of initial bulk velocity, while it plays a destructive role in other velocity ranges.     

等效原理算法在不同等效面下的误差分析

等效原理算法(EPA)借鉴了区域分解的思想,并基于惠更斯等效原理实现了对大规模散射问题的计算.本文对EPA中的等效原理算子(EPO)进行了误差分析,选取了立方体、球体和进行了光滑处理的立方体等作为等效面寻找误差来源,并提出了误差改进的建议.根据计算结果,等效磁流在等效面上描述的误差一般集中在等效面表面的几何不连续处,且主要受等效面上的法向向量不连续性和基函数的选取的影响.故在选取等效面时应尽量考虑使用平滑的等效面,而不是直接使用立方体等效面.      

Detection of microlesions induced by heavy ions using liposomes filled with fluorescent dye.

In cells irradiation by heavy ions has been hypothesized to produce microlesions, regions of local damage. In cell membranes this damage is thought to manifest itself in the form of holes. The primary evidence for microlesions comes from morphological studies of cell membranes, but this evidence is still controversial, especially since holes also have been observed in membranes of normal, nonirradiated, cells. However, it is possible that damage not associated with histologically discernable disruptions may still occur. In order to resolve this issue, we developed a system for detecting microlesions based on liposomes filled with fluorescent dye. We hypothesized that if microlesions form in these liposomes as the result of irradiation, then the entrapped dye will leak out into the surrounding medium in a measurable way. Polypropylene vials containing suspensions of vesicles composed of either dipalmitoyl phosphatidylcholine, or a combination of egg phosphatidylcholine and cholesterol were irradiated at the Brookhaven National Laboratory using 56Fe ions at 1 GeV/amu. In several cases we obtained a significant loss of the entrapped dye above the background level. Our results suggest that holes may form in liposomes as the result of heavy ion irradiation, and that these holes are large enough to allow leakage of cell internal contents that are at least as large as a 1 nm diameter calcein molecule.     

Ontogeny of plants under various gravity condition.

The results of experiments performed under conditions of microgravity (MG) or under its simulation on the horizontal clinostat (HC) with the callus, seedlings of various species and embryogenic structures have revealed a definite role of gravity as an ecological factor in the processes of cytomorphogenesis, growth, and development. The transformation of differentiated somatic cells of arabidopsis seed into undifferentiated callus was not inhibited under MG, though modifications of the whole callus morphology and of mean cell and nucleus size were observed. The morphogenesis of polar structures such as root-hair bearing cells of Lactuca primary root has been shown to be modified in the course of differentiation under mass acceleration diminished below 0.1 g. Seed germination and seedling morphogenesis under MG follow their normal course, but a significant stimulation of shoot growth with no effect on primary root growth has been determined. A successful in vitro regeneration of Nicotiana tabacum plantlets from leaf cells and subsequent formation of shoots and roots on a continuously rotating HC as well as the formation of viable seeds during seed-to-seed growth of Arabidopsis plants under MG have indicated that gravity plays but a limited role in the processes of embryogenesis and organogenesis.     

The first results from the Herschel-HIFI mission

This paper contains a summary of the results from the first years of observations with the HIFI instrument onboard ESA’s Herschel space observatory. The paper starts by outlining the goals and possibilities of far-infrared and submillimeter astronomy, the limitations of the Earth’s atmosphere, and the scientific scope of the Herschel-HIFI mission. The presentation of science results from the mission follows the life cycle of gas in galaxies as grouped into five themes: Structure of the interstellar medium, First steps in interstellar chemistry, Formation of stars and planets, Solar system results and Evolved stellar envelopes. The HIFI observations paint a picture where the interstellar medium in galaxies has a mixed, rather than a layered structure; the same conclusion may hold for protoplanetary disks. In addition, the HIFI data show that exchange of matter between comets and asteroids with planets and moons plays a large role. The paper concludes with an outlook to future instrumentation in the far-infrared and submillimeter wavelength ranges.     

Retinal photoreceptor and related gene expression in normal and clinostat-treated fish embryos

Medaka fish had performed mating behavior successfully in space for the first time among vertebrate, and the eggs which were laid in space developed normally, and hatched during the space travel of 15 days aboard the space shuttle in the second International Microgravity Laboratory (IML-2) mission in 1994 (Ijiri 1994). But there has been few studies whether microgravity affects the development of rather complex tissues in this fish. Investigating this problem, we focused on the organogenetic events in the retina in developing Medaka under normal and simulated microgravity conditions (by a three-dimensional clinostat, 3D-clinostat). Our results showed that both normal and 3D-clinostat-treated Medaka embryos developed on almost equal time course. Moreover, we investigated the development of the retina in normal and 3D-clinostat-treated embryos, but there were no differences in organogenesis of their retina. Lamination of retina occurred almost at equal timing and the expressions of opsin genes in the 3D-clinostat-treated group also began almost at the same time as control. Our observations suggest that there were no definite effects of simulated microgravity on the organizations of a complex tissue such as retina in developing fish embryos.     

基于有限元方法的蜘蛛网阻拦猎物过程模拟

为揭示蜘蛛网阻拦猎物过程的内在力学规律,基于理论和有限元方法对这一过程进行了研究。首先,根据已有实验结果建立了蜘蛛网径线和周线的应力-应变关系。然后,给出了单根丝线受横向冲击问题的理论解,并利用LS-DYNA有限元软件对该问题进行了数值模拟,有限元数值解与理论解一致。最后,利用LS-DYNA有限元软件模拟了蜘蛛网阻拦猎物的过程,得到的网内最大应变值和能量曲线与已有实验结果一致。计算结果表明,在蜘蛛网对猎物动能的耗散中,径线占主导地位;与冲击位置相邻的径线应力水平很低,有利于蜘蛛网的修复,径线中点附近的区域最有利于较大初始动能猎物的阻拦。      

Coronal heating mechanisms

As a result of the large body of data available from solar and stellar coronae, our understanding of the mechanisms responsible for the heating of coronal plasmas to temperatures of the order of ～ 10⁸ K has changed. The solar corona is highly structured by magnetic fields and the acoustic shocks which, according to early theories, should have acted as the coronal energy source have not been observed. Einstein Observatory data show moreover that coronae are present in most regions of the H-R diagram. The observed relationship between X-ray luminosity and rotational velocity in dwarf stars from spectral types F to M again suggests an active role for the magnetic fields.The basic picture which is emerging is that coronae in stellar types from F to M are produced because of the interaction of the magnetic field with the convective velocity fields generated in the photosphere resulting in MHD waves or currents which dissipate in the corona. X-ray emission in early type stars cannot be explained with this mechanism and the models which have been proposed for these stars are not yet completely satisfactory.