Adaptation of the macular vestibuloocular reflex to altered gravitational conditions in a fish (Oreochromis mossambicus).

Young fish (Oreochromis mossambicus) were exposed to microgravity (micro g) for 9 to 10 days, or to hypergravity (hg) for 9 days. For several weeks after termination of micro g and hg, the roll-induced static vestibuloocular reflex (rVOR) was recorded. In stage 11/12-fish, the rVOR amplitude (angle between the maximal up and down movement of an eye during a complete 360 degree lateral roll) of micro g-animals increased significantly by 25% compared to 1 g-controls during the first post-flight week but decreased to the control level during the second post-flight week. Microgravity had no effect in stage 14/16 fish on the rVOR amplitude. After 3 g-exposure, the rVOR amplitude was significantly reduced for both groups compared to their 1 g-controls. Readaptation to 1 g-condition was completed during the second post-3 g week. We postulate a critical period during which the development of the macular vestibuloocular reflex depends on gravitational input, and which is limited by the first appearance of the rVOR. At this period of early development, exposure to microgravity sensitizes the vestibular system while hypergravity desensitizes it.     

Features of vestibuloocular reflex modulations induced by altered gravitational forces in tadpoles (Xenopus laevis).

In Xenopus laevis tadpoles, we studied the static vestibuloocular reflex (rVOR) in relation to modifications of the gravitational environment to find basic mechanisms of how altered gravitational forces (AGF) affect this reflex. Animals were exposed to microgravity during space flight or hypergravity (3g) for 4 to 12 days. Basic observations were that (1)the development of the rVOR is significantly affected by altered gravitational conditions, (2) the duration of 1g-readaptation depends on the strength of the test stimulus, (3) microgravity induces malformations of the body which are related to the rVOR depression. Future studies are based on the hypotheses (1) that the vestibular nuclei play a key roll in the adaptation to AGF conditions, (2) that the stimulus transducing systems in the sense organ are affected by AGF conditions, and (3) that fertilized eggs will be converted to normal adults guided by physiological and morphological set points representing the genetic programs. Developmental retardation or acceleration, or otherwise occurring deviations from standard development during embryonic and postembryonic life will activate genes that direct the developmental processes towards normality.     

Low light intensity effects on the growth,photosynthetic characteristics,antioxidant capacity,yield and quality of wheat (Triticum aestivum L.) at different growth stages in BLSS

Minimizing energy consumption and maximizing crop productivity are major challenges to growing plants in Bioregenerative Life Support System (BLSS) for future long-term space mission. As a primary source of energy, light is one of the most important environmental factors for plant growth. The purpose of this study is to investigate the effects of low light intensity at different stages on growth, pigment composition, photosynthetic efficiency, biological production and antioxidant defence systems of wheat (Triticum aestivum L.) cultivars during ontogenesis. Experiments were divided into 3 intensity-controlled stages according to growth period (a total of 65 days): seedling stage (first 20 days), heading and flowering stage (middle 30 days) and grain filling stage (last 15 days). Initial light condition of the control was 420 μmol m⁻² s⁻¹ and the light intensity increased with the growth of wheat plants. The light intensities of group I and II at the first stage and the last stage were adjusted to the half level of the control respectively. For group III, the first and the last stage were both adjusted to half level of the control. During the middle 30 days, all treatments were kept the same intensity. The results indicated that low-light treatment at seedling stage, biomass, nutritional contents, components of inedible biomass and healthy index (including peroxidase (POD) activity, malondialdehyde (MDA) and proline content) of wheat plants have no significant difference to the control. Furthermore, unit kilojoule yield of group I reached 0.591 × 10⁻³ g/kJ and induced the highest energy efficiency. However, low-light treatment at grain filling stage affected the final production significantly.     

Far-UVC light as a new tool to reduce microbial burden during spacecraft assembly

This work aims to investigate far-UVC light at 222 nm as a new microbial reduction tool for planetary protection purposes which could potentially be integrated into the spacecraft assembly process. The major advantage of far-UVC (222 nm) compared to traditional germicidal UVC (254 nm) is the potential for application throughout the spacecraft assembly process in the presence of humans without adverse health effects due to the limited penetration of far-UVC light into biological materials. Testing the efficacy of 222-nm light at inactivating hardy bacterial cells and spores isolated from spacecraft and associated surfaces is a necessary step to evaluate this technology. We assessed survival of Bacillus pumilus SAFR-032 and Acinetobacter radioresistens 50v1 exposed to 222-nm light on proxy spacecraft surfaces simulated by drying the bacteria on aluminum coupons. The survival fraction of both bacteria followed a single stage decay function up to 60 mJ/cm², revealing similar susceptibility of both species to 222-nm light, which was independent of the exposure rate. Irradiation with far-UVC light at 222 nm is an effective method to decontaminate the proxy spacecraft materials tested in this study.     

Crop selection for advanced life support systems in the ESA MELiSSA program: Durum wheat (Triticum turgidum var durum)

As part of an ESA MELiSSA investigation into advanced life support (ALS) candidate crop cultivar selection and growth requirements, the University of Guelph’s Controlled Environment Systems Research Facility (CESRF) conducted a case study on growth and development of four durum wheat cultivars (Triticum turgidum var durum) grown hydroponically under controlled conditions in a sealed environment. Cultivars tested were Canadian developed Avonlea, Commander, Eurostar and Strongfield. There were few fundamental differences in durum quality parameters between hydroponically and field grown wheat, however yields of Eurostar and Strongfield exceeded those of field trials by 41% and 87% respectively.     

Low-latitude geomagnetic response to the interplanetary conditions during very intense magnetic storms

The variations in the horizontal and declination components of the geomagnetic field in response to the interplanetary shocks driven by fast halo coronal mass ejections, fast solar wind streams from the coronal hole regions and the dynamic pressure pulses associated with these events are studied. Close association between the field-aligned current density (j_∥) and the fluctuations in the declination component (ΔD_ABG) at Alibag is found for intense storm conditions. Increase in the dawn-dusk interplanetary electric field (E_y) and ΔD_ABG are generally in phase. However, when the magnetospheric electric field is directed from dusk to dawn direction, a prominent scatter occurs between the two. It is suggested that low-latitude ground magnetic data may serve as a proxy for the interplanetary conditions in the solar wind.     

Modelling of galactic Carbon in an asymmetrical heliosphere: Effects of asymmetrical modulation conditions

Observations of galactic cosmic rays (GCRs) from the two Voyager spacecraft inside the heliosheath indicate significant differences between them, suggesting that in addition to a possible global asymmetry in the north–south dimensions (meridional plane) of the heliosphere, it is also possible that different modulation (turbulence) conditions could exist between the two hemispheres of the heliosphere. We focus on illustrating the effects on GCR Carbon of asymmetrical modulation conditions combined with a heliosheath thickness that has a significant dependence on heliolatitude. To reflect different modulation conditions between the two heliospheric hemispheres in our numerical model, the enhancement of both polar and radial perpendicular diffusion off the ecliptic plane is assumed to differ from heliographic pole to pole. The computed radial GCR intensities at polar angles of 55° (approximating the Voyager 1 direction) and 125° (approximating the Voyager 2 direction) are compared at different energies and for both particle drift cycles. This is done in the context of illustrating how different values of the enhancement of both polar and radial perpendicular diffusion between the two hemispheres contribute to causing differences in radial intensities during solar minimum and moderate maximum conditions. We find that in the A > 0 cycle these differences between 55° and 125° change both quantitatively and qualitatively for the assumed asymmetrical modulation condition as reflected by polar diffusion, while in the A < 0 cycle, minute quantitative differences are obtained. However, when both polar and radial perpendicular diffusion have significant latitude dependences, major differences in radial intensities between the two polar angles are obtained in both polarity cycles. Furthermore, significant differences in radial intensity gradients obtained in the heliosheath at lower energies may suggest that the solar wind turbulence at and beyond the solar wind termination shock must have a larger latitudinal dependence.     

Acceleration of charged particles by fluctuating and steady electric fields in a X-type magnetic field

Release of stored magnetic energy via particle acceleration is a characteristic feature of astrophysical plasmas. Magnetic reconnection is one of the mechanisms for releasing energy from magnetized plasmas. Collisionless magnetic reconnection could provide both the energy release mechanism and the particle accelerator in space plasmas. Here we studied particle acceleration when fluctuating (in-time) electric fields are superposed on an static X-type magnetic field in collisionless hot solar plasma. This system is chosen to mimic the reconnective dissipation of a linear MHD disturbance. Our results are compared to particle acceleration from constant electric field superposed on an X-type magnetic field. The constant electric field configuration represents the effects of steady state magnetic reconnection. Time evolution of ion and electron distributions are obtained by numerically integrating particle trajectories. The frequencies of the electric field represent a turbulent range of waves. Depending on the frequency and amplitude of the electric field, electrons and ions are accelerated to different degrees and have energy distributions of bimodal form consisting of a lower energy part and a high energy tail. For frequencies (ω in dimensioless units) in the range 0.5 ? ω ? 1.0 a substantial fraction (20%–30%) of the proton distribution is accelerated to gamma-ray producing energies. For frequencies in the range 1 ? ω ? 100.0 the bulk of the electron distribution is accelerated to hard X-ray producing energies. The acceleration mechanism is important for solar flares and solar noise storms but it could be applicable to all collisionless astrophysical plasmas.     

Characterizing parameters of Jatropha curcas cell cultures for microgravity studies

Jatropha (Jatropha curcas) is a tropical perennial species identified as a potential biofuel crop. The oil is of excellent quality and it has been successfully tested as biodiesel and in jet fuel mixes. However, studies on breeding and genetic improvement of jatropha are limited. Space offers a unique environment for experiments aiming at the assessment of mutations and differential gene expression of crops and in vitro cultures of plants are convenient for studies of genetic variation as affected by microgravity. However, before microgravity studies can be successfully performed, pre-flight experiments are necessary to characterize plant material and validate flight hardware environmental conditions. Such preliminary studies set the ground for subsequent spaceflight experiments. The objectives of this study were to compare the in vitro growth of cultures from three explant sources (cotyledon, leaf, and stem sections) of three jatropha accessions (Brazil, India, and Tanzania) outside and inside the petriGAP, a modified group activation pack (GAP) flight hardware to fit petri dishes. In vitro jatropha cell cultures were established in petri dishes containing a modified MS medium and maintained in a plant growth chamber at 25 ± 2 °C in the dark. Parameters evaluated were surface area of the explant tissue (A), fresh weight (FW), and dry weight (DW) for a period of 12 weeks. Growth was observed for cultures from all accessions at week 12, including subsequent plantlet regeneration. For all accessions differences in A, FW and DW were observed for inside vs. outside the PetriGAPs. Growth parameters were affected by accession (genotype), explant type, and environment. The type of explant influenced the type of cell growth and subsequent plantlet regeneration capacity. However, overall cell growth showed no abnormalities. The present study demonstrated that jatropha in vitro cell cultures are suitable for growth inside PetriGAPs for a period of 12 weeks. The parameters evaluated in this study provide the basic ground work and pre-flight assessment needed to justify a model for microgravity studies with jatropha in vitro cell cultures. Future studies should focus on results of experiments performed with jatropha in vitro cultures in microgravity.     

On the source region of flux transfer events

We have examined the region of occurrence of flux transfer events for three distinct orientations of the interplanetary magnetic field: nearly horizontal in the solar magnetospheric equator, diagonally southward at 45° to the magnetospheric equator and nearly due south. For horizontal IMF conditions the FTE's occur in a horizontal band about ± 6 R_E wide. For diagonally southward IMF conditions, the FTE's occur in a diagonal swath about ± 6 R_E wide passing through the subsolar point. For duskward but nearly due southward IMF conditions, our observations reveal FTE's throughout the northern morning quadrant. These observations are consistent with a near equatorial source for flux transfer events and hence with component merging and not anti-parallel merging. These observations also help understand the energetic ion anisotropies seen in these events.     

The TUS space fluorescence detector for study of UHECR and other phenomena of variable fluorescence light in the atmosphere

The Tracking Ultraviolet Set Up (TUS) instrument has been designed to observe from space the fluorescence light in the atmosphere when Extensive Air Shower (EAS) or other phenomena such as meteors or dust grains traverse it. The TUS design concepts will allow us to construct the next generation of fluorescence detectors with increasing light collection power and higher resolution. The KLYPVE instrument with collection power 5 times larger of the TUS will be the next space detector. Light collection is obtained with the help of segmented “low frequency Fresnel type” mirrors. Photo receiver retina in the focal consists of modules of PM tubes. For stable performance in conditions of variable light noise and variable temperature the tube type with a multi-alcali cathode was chosen. Voltage supplies for PMT in one module were designed for keeping the performance of photo receiver retina uniform when the tube gain change. From every tube the signal amplitude is recorded in time bins of 400 ns. The digital data are kept and analyzed in the module FPGA connected to the central FPGA controlling all data. The RAM memory is large, capable to record events with different duration of the light signal (up to several seconds). The preliminary event data are analyzed in the triggering system of the central FPGA. The trigger criteria have several options for events of different origin (different pixel signal duration). The trigger integration time is controlled from the space mission center. The performances of the detector were simulated and zenith angle dependent trigger efficiencies were calculated. The TUS detector will be efficient in recording “horizontal” EAS (zenith angles more than 60°), developed to their maximum above the cloud cover. The EAS Cherenkov light, back scattered from the cloud cover, will be recorded and will improve data on the EAS direction and position of maximum. For better accuracy in physical parameters of the events and for the experimental check of this accuracy the performance of two TUS detectors at the space platform was recommended. The accommodation of 2 TUS detectors at space platform of the “RESURS O” type was tried and approved. The TUS prototypes are being tested in the Mexican mountains. The photo receiver of two PM tubes with the TUS electronics on-board of the MSU Tatiana satellite is measuring the atmosphere light background.     

Improvements in the re-flight of spaceflight experiments on plant tropisms

In order to effectively study phototropism, the directed growth in response to light, we performed a series of experiments in microgravity to better understand light response without the “complications” of a 1-g stimulus. These experiments were named TROPI (for tropisms) and were performed on the European Modular Cultivation System (EMCS), a laboratory facility on the International Space Station (ISS). TROPI-1 was performed in 2006, and while it was a successful experiment, there were a number of technical difficulties. We had the opportunity to perform TROPI-2 in 2010 and were able to optimize experimental conditions as well as to extend the studies of phototropism to fractional gravity created by the EMCS centrifuge. This paper focuses on how the technical improvements in TROPI-2 allowed for a better experiment with increased scientific return. Major modifications in TROPI-2 compared to TROPI-1 included the use of spaceflight hardware that was off-gassed for a longer period and reduced seed storage (less than 2 months) in hardware. These changes resulted in increased seed germination and more vigorous growth of seedlings. While phototropism in response to red illumination was observed in hypocotyls of seedlings grown in microgravity during TROPI-1, there was a greater magnitude of red-light-based phototropic curvature in TROPI-2. Direct downlinking of digital images from the ISS in TROPI-2, rather than the use of analog tapes in TROPI-1, resulted in better quality images and simplified data analyses. In TROPI-2, improved cryo-procedures and the use of the GLACIER freezer during transport of samples back to Earth maintained the low temperature necessary to obtain good-quality RNA required for use in gene profiling studies.     

Swimming behaviour of the upside-down swimming catfish (Synodontis nigriventris) at high-quality microgravity – A drop-tower experiment

The catfish Synodontis nigriventris often shows a unique swimming behaviour in being oriented upside-down. When swimming near a (e.g., vertical) substrate, however, the animals orient themselves with their ventral side towards this substrate. This tendency is called ventral substrate response (VSR). The VSR does not only override the upside-down swimming behaviour but also the dorsal light response and the ventral light response.     

A solar wind driven model of geosynchronous plasma moments

We describe a tabular specification model of the density and temperature of ions and electrons at geosynchronous orbit as a function of magnetic local time and solar wind parameters. This model can be used to provide boundary conditions for numerical ring current models. Unlike previous specification models of geosynchronous plasma moments, this model is parameterized by upstream solar wind conditions. We find that solar wind parameters are a better predictor of geosynchronous ion density than magnetospheric indices, and as upstream parameters they are often more appropriate as model inputs since they causally precede the model outputs. Of the upstream parameters that were tested, the best predictors of geosynchronous conditions were the solar wind flow pressure and the magnitude and Z-component of the interplanetary magnetic field.     

Long-wavelength lunar gravity field recovery from simulated orbit and inter-satellite tracking data

As has been demonstrated recently, inter-satellite Ka-band tracking data collected by the GRAIL (Gravity Recovery And Interior Laboratory) spacecraft have the potential to improve the resolution and accuracy of the lunar gravity field by several orders of magnitude compared to previous models. By means of a series of simulation studies, here we investigate the contribution of inter-satellite ranging for the recovery of the Moon’s gravitational features; the evaluation of results is made against findings from ground-based Doppler tracking. For this purpose we make use of classical dynamic orbit determination, supported by the analysis of satellite-to-satellite tracking observations. This study sheds particularly light on the influence of the angular distance between the two satellites, solar radiation modeling and the co-estimation of the lunar Love number k₂. The quality of the obtained results is assessed by gravity field power spectra, gravity anomalies and precision orbit determination. We expect our simulation results to be supportive for the processing of real GRAIL data.     

Influence of the interaction between light intensity and CO2 concentration on productivity and quality of spinach (Spinacia oleracea L.) grown in fully controlled environment

The effects of the factorial combination of two light intensities (200 and 800 μmol m⁻² s⁻¹) and two CO₂ concentrations (360 and 800 ppm) were studied on the productivity and nutritional quality of spinach (Spinacia oleracea L.) grown under controlled environment. After 6 weeks within a growth chamber, spinach plants were sampled and analyzed for productivity and quality. There were no statistically significant interactions between the effects of light and CO₂ for all of the variables studied, except for the nitrate and oxalic acid content of the leaves. High light and high CO₂ independently one from the other, promoted spinach productivity, and the accumulation of ascorbic acid, while their interactive effect limited the accumulation of nitrate and oxalic acid in the spinach leaves. The results highlight the importance of considering the effects of the interaction among environmental variables on maximizing production and the nutritional quality of the food when cultivating and modeling the plant response in controlled environment systems such as for bioregenerative life support.     

Community metabolism of aquatic Closed Ecological Systems: Effects of nitrogen sources

To investigate the effect of nitrogen sources on Closed Ecological Systems (CESs), three nitrogen sources (NaNO₃, sodium nitrate; NH₄Cl, ammonium chloride; and NH₄NO₃, ammonium nitrate) were each tested in freshwater CESs consisting of a chemically defined medium, three species of green algae (Ankistrodesmus, Scenedesmus, and Selenastrum), the grazer Daphnia magna, and associated microbes, under 12 h light/12 h dark cycles. It had been hypothesized that the development of high pH in earlier CESs was the result of nitrate utilization, and that ammonium might result in acid conditions, while ammonium nitrate might result in more moderate pH. The three nitrogen sources supported similar densities of algae (estimated by in vivo fluorescence) and similar Daphnia populations. The experiments showed that pH levels rapidly increased when grazers were absent or at low abundances irrespective of the nitrogen source. Consequently, it is hypothesized that carbon cycles, rather than nitrogen sources, are responsible for the pH dynamics. Oxygen diurnal (light:dark) cycles tended to come into balance more quickly than pH. It may be more feasible to convert O₂ data to energy units (using “oxycalorific” values) than CO₂ data since CO₂ dynamics may include other chemical reactions than just photosynthesis and respiration. The feasibility of sustaining grazer populations for at least several weeks in small, simple CESs was demonstrated, along with the ability to monitor algae-grazer dynamics, and the recording of O₂ and pH measurements.     

Phytochromes play a role in phototropism and gravitropism in Arabidopsis roots.

Phototropism as well as gravitropism plays a role in the oriented growth of roots in flowering plants. In blue or white light, roots exhibit negative phototropism, but red light induces positive phototropism in Arabidopsis roots. Phytochrome A (phyA) and phyB mediate the positive red-light-based photoresponse in roots since single mutants (and the double phyAB mutant) were severely impaired in this response. In blue-light-based negative phototropism, phyA and phyAB (but not phyB) were inhibited in the response relative to the WT. In root gravitropism, phyB and phyAB (but not phyA) were inhibited in the response compared to the WT. The differences observed in tropistic responses were not due to growth limitations since the growth rates among all the mutants tested were not significantly different from that of the WT. Thus, our study shows that the blue-light and red-light systems interact in roots and that phytochrome plays a key role in plant development by integrating multiple environmental stimuli.     

The gravitropic and phototropic responses of wheat grown in a space greenhouse prototype with hemispherical planting surface

The time course of gravicurvature of 3-day-old wheat (Triticum aestivum L., cv. Apogee) coleoptiles and 7-day-old wheat stems were studied in darkness and under red and red-blue light illumination after declination from the vertical at various angles. The experiments showed that the shortest gravitropic curvature corresponded to 30° initial angle of gravistimulation (IAG). The time course became longer as the IAG increased and with plant age. The effects of unilateral red (660 nm) and red-blue light (660 nm; 470 nm) at photosynthetic photon flux (PPF) of 30 μmol m⁻² s⁻¹ on the curvature of 3-day-old coleoptiles were evaluated. Red light did not produce phototropic bending of wheat coleoptiles in contrast with red-blue light. The analysis of experimental data showed that the curvature in response to a gravitropic stimulus or to combined gravity-light stimuli were not statistically different. Time course of gravitropic curvature were used to determine the acceptable crop rotation rate around the horizontal axis. Approximation of stem bending to a linear dynamic system described by a first-order aperiodic element with a lag allowed the determination of the dependence of the amplitude of apex oscillations on the rate of horizontal rotation under 1-g conditions. The calculated lowest minimal rotation rate (MRR) minimizing the gravitropic effects on wheat was about 1 revolution per hour (rph). Rotating the plant growth chamber (PGC) at a rate of more than MRR eliminated the effect of gravitropic curvature.