载人深空探测活动中的尿液处理回收技术分析

生物再生生命保障系统(Bioregenerative Life Support System,BLSS)是人类进行深空探测活动,实现长期载人空间飞行必需的关键技术,对于太空的探索开发具有重要意义。在BLSS系统内,航天员尿液废水的处理回收是非常重要的一部分。将尿液中所含有的大量的水分和丰富的营养物质回收用于系统内植物生长所需营养液的配制,既可以保证植物的正常生长,也有助于实现系统内物质的循环利用进而提高BLSS的闭合度。尿液中所含的大量盐分会威胁植物生长,所以需通过一定的技术手段处理尿液废水并回收其中的水分和营养。为了探索适用于BLSS中的尿液处理回收技术,首先分析了几种面向空间站应用的尿液处理技术,如蒸馏技术等;然后基于回收营养物质的需求,分析了面向民用的、发展较为成熟的尿液处理回收技术,如离子交换吸附技术、氨气吹脱技术和鸟粪石沉淀技术,并讨论了这些尿液处理回收技术在BLSS中的应用前景。最后基于BLSS的实际需求,提出了有望用于BLSS中的尿液处理回收技术流程。     

空间站废水的处理及管理

长期载入飞行的空间站需要大量的用水，为解决由此引起的巨大的维持飞行的运行费用，需要实现水的再生与闭路循环，如何针对空间站废水的特征，解决废水的处理与利用问题，并建立合理的水循环体系是这一工作的关键，文中分析，比较了空间站废水的处理方法，设计了空间站水管理整合方案，并多方面探讨和验证这一方案的合理性和可行性。     

Increased BLSS closure using mineralized human waste in plant cultivation on a neutral substrate

The purpose of this work was to study the full-scale potential use of human mineralized waste (feces and urine) as a source of mineral elements for plant cultivation in a biological life support system (BLSS). Plants that are potential candidates for a photosynthesizing link were grown on a neutral solution containing human mineralized waste. Spring wheat Triticum aestivum L., peas Pisum sativum L. Ambrosia cultivar and leaf lettuce Lactuca sativa L., Vitaminny variety, were used. The plants were grown hydroponically on expanded clay aggregates in a vegetation chamber in constant environmental conditions. During plant growth, a determined amount of human mineralized waste was added daily to the nutrient solution. The nutrient solution remained unchanged throughout the vegetation period. Estimated plant requirements for macro-elements were based on a total biological productivity of 0.04 kg day⁻¹ m⁻². As the plant requirements for potassium exceeded the potassium content of human waste, a water extract of wheat straw containing the required amount of potassium was added to the nutrient solution. The Knop’s solution was used in the control experiments.     

Biological dosimetry to determine the UV radiation climate inside the MIR station and its role in vitamin D biosynthesis

The vitamin D synthesis in the human skin, is absolutely dependent on UVB radiation. Natural UVB from sunlight is normally absent in the closed environment of a space station like MIR. Therefore it was necessary to investigate the UV radiation climate inside the station resulting from different lamps as well as from occasional solar irradiation behind a UV-transparent quartz window. Biofilms, biologically weighting and integrating UV dosimeters successfully applied on Earth (e.g. in Antarctica) and in space (D-2, Biopan I) were used to determine the biological effectiveness of the UV radiation climate at different locations in the space station. Biofilms were also used to determine the personal UV dose of an individual cosmonaut. These UV data were correlated with the concentration of vitamin D in the cosmonaut's blood and the dietary vitamin D intake. The results showed that the UV radiation climate inside the Mir station is not sufficient for an adequate supply of vitamin D, which should therefore be secured either by vitamin D supplementat and/or by the regular exposure to special UV lamps like those in sun-beds. The use of natural solar UV radiation through the quartz window for ‘sunbathing’ is dangerous and should be avoided even for short exposure periods.     

Closed bioregenerative life support systems: Applicability to hot deserts

Water scarcity in hot deserts, which cover about one-fifth of the Earth’s land area, along with rapid expansion of hot deserts into arable lands is one of the key global environmental problems. As hot deserts are extreme habitats characterized by the availability of solar energy with a nearly complete absence of organic life and water, space technology achievements in designing closed ecological systems may be applicable to the design of sustainable settlements in the deserts. This review discusses the key space technology findings for closed biogenerative life support systems (CBLSS), which can simultaneously produce food, water, nutrients, fertilizers, process wastes, and revitalize air, that can be applied to hot deserts. Among them are the closed cycle of water and the acceleration of the cycling times of carbon, biogenic compounds, and nutrients by adjusting the levels of light intensity, temperature, carbon dioxide, and air velocity over plant canopies. Enhanced growth of algae and duckweed at higher levels of carbon dioxide and light intensity can be important to provide complete water recycling and augment biomass production. The production of fertilizers and nutrients can be enhanced by applying the subsurface flow wetland technology and hyper-thermophilic aerobic bacteria for treating liquid and solid wastes. The mathematical models, optimization techniques, and non-invasive measuring techniques developed for CBLSS make it possible to monitor and optimize the performance of such closed ecological systems. The results of long-duration experiments performed in BIOS-3, Biosphere 2, Laboratory Biosphere, and other ground-based closed test facilities suggest that closed water cycle can be achieved in hot-desert bioregenerative systems using the pathways of evapotranspiration, condensation, and biological wastewater treatment technologies. We suggest that the state of the art in the CBLSS design along with the possibility of using direct sunlight for photosynthesis and recent advances in photovoltaic engineering can be used as a basis for building sustainable settlements producing food, water, and energy in hot deserts.     

Biologically weighted measurement of UV radiation in space and on Earth with the biofilm technique.

Biological dosimetry has provided experimental proof of the high sensitivity of the biologically effective UVB doses to changes in atmospheric ozone and has thereby confirmed the predictions from model calculations. The biological UV dosimeter 'biofilm' whose sensitivity is based on dried spores of B. subtilis as UV target weights the incident UV radiation according to its DNA damaging potential. Biofilm dosimetry was applicated in space experiments as well as in use in remote areas on Earth. Examples are long-term UV measurements in Antarctica, measurements of diurnal UV profiles parallel in time at different locations in Europe, continuous UV measurements in the frame of the German UV measurement network and personal UV dosimetry. In space biofilms were used to determine the biological efficiency of the extraterrestrial solar UV, to simulate the effects of decreasing ozone concentrations and to determine the interaction of UVB and vitamin D production of cosmonauts in the MIR station.     

Survival of microorganisms in space protected by meteorite material: results of the experiment 'EXOBIOLOGIE' of the PERSEUS mission.

During the early evolution of life on Earth, before the formation of a protective ozone layer in the atmosphere, high intensities of solar UV radiation of short wavelengths could reach the surface of the Earth. Today the full spectrum of solar UV radiation is only experienced in space, where other important space parameters influence survival and genetic stability additionally, like vacuum, cosmic radiation, temperature extremes, microgravity. To reach a better understanding of the processes leading to the origin, evolution and distribution of life we have performed space experiments with microorganisms. The ability of resistant life forms like bacterial spores to survive high doses of extraterrestrial solar UV alone or in combination with other space parameters, e.g. vacuum, was investigated. Extraterrestrial solar UV was found to have a thousand times higher biological effectiveness than UV radiation filtered by stratospheric ozone concentrations found today on Earth. The protective effects of anorganic substances like artificial or real meteorites were determined on the MIR station. In the experiment EXOBIOLOGIE of the French PERSEUS mission (1999) it was found that very thin layers of anorganic material did not protect spores against the deleterious effects of energy-rich UV radiation in space to the expected amount, but that layers of UV radiation inactivated spores serve as a UV-shield by themselves, so that a hypothetical interplanetary transfer of life by the transport of microorganisms inside rocks through the solar system cannot be excluded, but requires the shielding of a substantial mass of anorganic substances.     

Factors controlling activity of zirconia-titania for photocatalytic oxidation of ethylene.

Small photocatalytic devices were developed to remove ethylene from closed plant growth units flown in space. The devices utilized sol-gel-derived catalyst pellets of zirconia-titania. This study was undertaken to understand the significance of different factors on the photocatalytic activity of the catalyst. Increasing reaction temperatures and decreasing humidity of the air significantly increased oxidation of ethylene. The quantity of ethylene oxidized per unit time increased linearly with increasing flow rates, and increasing concentrations of ethylene. Zirconia-titania pellet size and heel depth had little effect on oxidation of ethylene. Platinizing the zirconia-titania significantly increased ethylene oxidation. The catalyst was found to absorb large quantities of water when the humidity of the air stream was elevated and this greatly decreased catalytic activity.     

Creation of biological module for self-regulating ecological system by the way of polymerization of composite materials in free space.

The large-size frame of space ship and space station can be created with the use of the technology of the polymerization of fiber-filled composites and a liquid reactionable matrix applied in free space or on the other space body when the space ship or space station will be used during a long period of time. For the polymerization of the station frame the fabric impregnated with a long-life polymer matrix (prepreg) is prepared in terrestrial conditions and, after folding, can be shipped in a compact container to orbit and kept folded on board the station. In due time the prepreg is carried out into free space and unfolded. Then a reaction of matrix polymerization starts. After reaction of polymerization the durable frame is ready for exploitation. After that, the frame can be filled out with air, the apparatus and life support systems. The technology can be used for creation of biological frame as element of self regulating ecological system, and for creation of technological frame which can be used for a production of new materials on Earth orbit in microgravity conditions and on other space bodies (Mars, Moon, asteroids) for unique high price mineral extraction. Based on such technology a future space base on Earth orbit with volume of 10(6) m3 and a crew of 100 astronauts is considered.     

Canadian advanced life support capacities and future directions

Canada began research on space-relevant biological life support systems in the early 1990s. Since that time Canadian capabilities have grown tremendously, placing Canada among the emerging leaders in biological life support systems. The rapid growth of Canadian expertise has been the result of several factors including a large and technically sophisticated greenhouse sector which successfully operates under challenging climatic conditions, well planned technology transfer strategies between the academic and industrial sectors, and a strong emphasis on international research collaborations. Recent activities such as Canada’s contribution of the Higher Plant Compartment of the European Space Agency’s MELiSSA Pilot Plant and the remote operation of the Arthur Clarke Mars Greenhouse in the Canadian High Arctic continue to demonstrate Canadian capabilities with direct applicability to advanced life support systems. There is also a significant latent potential within Canadian institutions and organizations with respect to directly applicable advanced life support technologies. These directly applicable research interests include such areas as horticultural management strategies (for candidate crops), growth media, food processing, water management, atmosphere management, energy management, waste management, imaging, environment sensors, thermal control, lighting systems, robotics, command and data handling, communications systems, structures, in-situ resource utilization, space analogues and mission operations. With this background and in collaboration with the Canadian aerospace industry sector, a roadmap for future life support contributions is presented here. This roadmap targets an objective of at least 50% food closure by 2050 (providing greater closure in oxygen, water recycling and carbon dioxide uptake). The Canadian advanced life support community has chosen to focus on lunar surface infrastructure and not low Earth orbit or transit systems (i.e. microgravity applications). To advance the technical readiness for the proposed lunar missions, including a lunar plant growth lander, lunar “salad machine” (i.e. small scale plant production unit) and a full scale lunar plant production system, a suite of terrestrial developments and analogue systems are proposed. As has been successfully demonstrated by past Canadian advanced life support activities, terrestrial technology transfer and the development of highly qualified personnel will serve as key outputs for Canadian advanced life support system research programs. This approach is designed to serve the Canadian greenhouse industry by developing compliance measures for mitigating environmental impact, reducing labour and energy costs as well as improving Canadian food security, safety and benefit northern/remote communities.     

Use of human wastes oxidized to different degrees in cultivation of higher plants on the soil-like substrate intended for closed ecosystems

To close mass exchange loops in bioregenerative life support systems more efficiently, researchers of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) have developed a procedure of wet combustion of human wastes and inedible parts of plants using H₂O₂ in alternating electromagnetic field. Human wastes pretreated in this way can be used as nutrient solutions to grow plants in the phototrophic unit of the LSS. The purpose of this study was to explore the possibilities of using human wastes oxidized to different degrees to grow plants cultivated on the soil-like substrate (SLS). The treated human wastes were analyzed to test their sterility. Then we investigated the effects produced by human wastes oxidized to different degrees on growth and development of wheat plants and on the composition of microflora in the SLS. The irrigation solution contained water, substances extracted from the substrate, and certain amounts of the mineralized human wastes. The experiments showed that the human wastes oxidized using reduced amounts of 30% H₂O₂: 1 ml/g of feces and 0.25 ml/ml of urine were still sterile. The experiments with wheat plants grown on the SLS and irrigated by the solution containing treated human wastes in the amount simulating 1/6 of the daily diet of a human showed that the degree of oxidation of human wastes did not significantly affect plant productivity. On the other hand, the composition of the microbiota of irrigation solutions was affected by the oxidation level of the added metabolites. In the solutions supplemented with partially oxidized metabolites yeast-like microscopic fungi were 20 times more abundant than in the solutions containing fully oxidized metabolites. Moreover, in the solutions containing incompletely oxidized human wastes the amounts of phytopathogenic bacteria and denitrifying microorganisms were larger. Thus, insufficiently oxidized sterile human wastes added to the irrigation solutions significantly affect the composition of the microbiological component of these solutions, which can ultimately unbalance the system as a whole.     

Biological and physiochemical methods for utilization of plant wastes and human exometabolites for increasing internal cycling and closure of life support systems.

Wheat was cultivated on soil-like substrate (SLS) produced by the action of worms and microflora from the inedible biomass of wheat. After the growth of the wheat crop, the inedible biomass was restored in SLS and exposed to decomposition ("biological" combustion) and its mineral compounds were assimilated by plants. Grain was returned to the SLS in the amount equivalent to human solid waste produced by consumption of the grain. Human wastes (urine and feces) after physicochemical processing turned into mineralized form (mineralized urine and mineralized feces) and entered the plants' nutrient solution amounts equal to average daily production. Periodically (once every 60-70 days) the nutrient solution was partly (up to 50%) desalinated by electrodialysis. Due to this NaCl concentration in the nutrient solution was sustained at a fixed level of about 0.26%. The salt concentrate obtained could be used in the human nutrition through NaCl extraction and the residuary elements were returned through the mineralized human liquid wastes into matter turnover. The control wheat cultivation was carried out on peat with use of the Knop nutrient solution. Serial cultivation of several wheat vegetations within 280 days was conducted during the experiment. Grain output varied and yield/harvest depended, in large part, upon the amount of inedible biomass returned to SLS and the speed of its decomposition. After achieving a stationary regime, (when the quantity of wheat inedible biomass utilized during vegetation in SLS is equal to the quantity of biomass introduced into SLS before vegetation) grain harvest in comparison with the control was at most 30% less, and in some cases was comparable to the control harvest values. The investigations carried out on the wheat example demonstrated in principle the possibility of long-term functioning of the LSS photosynthesizing link based on optimizations of biological and physicochemical methods of utilization of the human and plants wastes. The possibilities for the use of these technologies for the creation integrated biological-physicochemical LSS with high closure degree of internal matter turnover are discussed in this paper.     

Radiation protection problems for the space station and approaches to their mitigation.

With the advent of a permanent manned space station the longstanding problems of radiation protection in manned spaceflight have acquired an immediacy. This paper endeavors to emphasize the gaps of our knowledge which must be closed for effective radiation protection. The information that is required includes the accurate determination of the exposure inside the space station to the various components of tile ionizing radiation, the evaluation of the biological importance of the different radiation qualities and the depth dose distribution of the less penetrating component. There is also the possibility of an interaction with weightlessness. It is necessary to establish adequate radiation protection standards and a system of dosimetric surveillance. There is a need for studies of possible methods of hardening selective shielding of the space station. Spaceflight experiments, which might contribute to the solution of some of these problems are discussed.     

Subcritical and supercritical water oxidation of CELSS model wastes.

Controlled-Ecological-Life-Support-System (CELSS) model wastes were wet-oxidized at temperatures from 250 to 500 degrees C, i.e., below and above the critical point of water (374 degrees C and 218 kg/cm2 or 21.4 MPa). A solution of ammonium hydroxide and acetic acid and a slurry of human urine, feces, and wipes were used as model wastes. Almost all of the organic matter in the model wastes was oxidized in the temperature range from 400 to 500 degrees C, i.e., above the critical conditions for water. In contrast, only a small portion of the organic matter was oxidized at subcritical conditions. Although the extent of nitrogen oxidation to nitrous oxide (N2O) and/or nitrogen gas (N2) increased with reaction temperature, most of the nitrogen was retained in solution as ammonia near 400 degrees C. This important finding suggests that most of the nitrogen in the waste feed can be retained in solution as ammonia during oxidation at low supercritical temperatures and be subsequently used as a nitrogen source for plants in a CELSS while at the same time organic matter is almost completely oxidized to carbon dioxide and water. It was also found in this study the Hastelloy C-276 alloy reactor corroded during waste oxidation. The rate of corrosion was lower above than below the critical temperature for water.     

Aquaculture in bio-regenerative life support systems (BLSS): Considerations

A significant amount of research has been invested into understanding the effects of including fish culture in bio-regenerative life support systems (BLSS) for long duration space habitation. While the benefits of fish culture as a sub-process for waste treatment and food production continue to be identified, other pressing issues arise that affect the overall equivalent system mass associated with fish culture in a BLSS. This paper is meant to provide insight into several issues affecting fish culture in a BLSS that will require attention in the future if fish meant for consumption are to be cultured in a BLSS.     

A bioreactor system for the nitrogen loop in a Controlled Ecological Life Support System.

As space missions become longer in duration, the need to recycle waste into useful compounds rises dramatically. This problem can be addressed by the development of Controlled Ecological Life Support Systems (CELSS) (i.e., Engineered Closed/Controlled Eco-Systems (ECCES)), consisting of human and plant modules. One of the waste streams leaving the human module is urine. In addition to the reclamation of water from urine, recovery of the nitrogen is important because it is an essential nutrient for the plant module. A 3-step biological process for the recycling of nitrogenous waste (urea) is proposed. A packed-bed bioreactor system for this purpose was modeled, and the issues of reaction step segregation, reactor type and volume, support particle size, and pressure drop were addressed. Based on minimization of volume, a bioreactor system consisting of a plug flow immobilized urease reactor, a completely mixed flow immobilized cell reactor to convert ammonia to nitrite, and a plug flow immobilized cell reactor to produce nitrate from nitrite is recommended. It is apparent that this 3-step bioprocess meets the requirements for space applications.     

Conceptual design of a bioregenerative life support system containing crops and silkworms

This article summarizes a conceptual design of a bioregenerative life support system for permanent lunar base or planetary exploration. The system consists of seven compartments – higher plants cultivation, animal rearing, human habitation, water recovery, waste treatment, atmosphere management, and storages. Fifteen kinds of crops, such as wheat, rice, soybean, lettuce, and mulberry, were selected as main life support contributors to provide the crew with air, water, and vegetable food. Silkworms fed by crop leaves were designated to produce partial animal nutrition for the crew. Various physical-chemical and biological methods were combined to reclaim wastewater and solid waste. Condensate collected from atmosphere was recycled into potable water through granular activated carbon adsorption, iodine sterilization, and trace element supplementation. All grey water was also purified though multifiltration and ultraviolet sterilization. Plant residue, human excrement, silkworm feces, etc. were decomposed into inorganic substances which were finally absorbed by higher plants. Some meat, ingredients, as well as nitrogen fertilizer were prestored and resupplied periodically. Meanwhile, the same amount and chemical composition of organic waste was dumped to maintain the steady state of the system. A nutritional balanced diet was developed by means of the linear programming method. It could provide 2721 kcal of energy, 375.5 g of carbohydrate, 99.47 g of protein, and 91.19 g of fat per capita per day. Silkworm powder covered 12.54% of total animal protein intakes. The balance of material flows between compartments was described by the system of stoichiometric equations. Basic life support requirements for crews including oxygen, food, potable and hygiene water summed up to 29.68 kg per capita per day. The coefficient of system material closure reached 99.40%.     

空间微生物实验技术研究进展

微生物具有结构简单、生长周期短、繁殖快、便于搭载等优点,被作为生物模型用于空间环境下的生命现象研究、地外生命探测以及开展以微生物为重点关注对象的行星保护任务研究.随着中国空间站在轨建造任务的临近,微生物空间实验技术开发以及相应的实验装置研制需要加快部署.本文以已实现空间应用的微生物技术为基础,从微生物空间培养、保存、检...     

Bioelectrochemical degradation of urea at platinized boron doped diamond electrodes for bioregenerative systems

The recovery of potable water from space mission wastewater is critical for the life support and environmental health of crew members in long-term missions. NASA estimates reveal that at manned space missions 1.91 kg/person day of urine is produced, with urea and various salts as its main components. In this research we explore the utilization of urease (EC 3.5.1.5, 15,000 U/g) along with a platinized boron doped diamond electrode (Pt-BDD) to degrade urea. Urea is directly degraded to nitrogen by the in situ utilization of the reaction products as a strategy to increase the amount of clean water in future space expeditions. The biochemical reaction of urease produces ammonia and carbon dioxide from urea. Thereafter, ammonia is electrooxidized at the interface of the Pt-BDD producing molecular nitrogen. The herein presented system has been proven to have 20% urea conversion efficiency. This research has potential applications for future long-term space missions since the reaction byproducts could be used for a biomass subsystem (in situ resource recovery), while generating electricity from the same process.     

Analysis of the DORIS contributions to ITRF2008

In its function as an ITRS Combination Centre, DGFI is in charge with the computation of an ITRF2008 solution. The computation methodology of DGFI is based on the combination of datum-free normal equations (weekly or session data sets, respectively) of station positions and Earth orientation parameters (EOP) from the geodetic space techniques DORIS, GPS, SLR and VLBI. In this paper we focus on the DORIS part within the ITRF2008 computations. We present results obtained from the analysis of the DORIS time series for station positions, network translation and scale parameters, as well as for the terrestrial pole coordinates. The submissions to ITRF2008 benefit from improved analysis strategies of the seven contributing IDS analysis centres and from a combination of the weekly solutions of station positions and polar motion. The results show an improvement by a factor of two compared to past DORIS data submitted to ITRF2005, which has been evaluated by investigating the repeatabilities of position time series. The DORIS position time series were analysed w.r.t. discontinuities and other non-linear effects such as seasonal variations. About 40 discontinuities have been identified which have been compared with the results of an earlier study. Within the inter-technique combination we focus on the DORIS contribution to the integration of the different space geodetic observations and on a comparison of the geodetic local ties with the space geodetic solutions. Results are given for the 41 co-location sites between DORIS and GPS.