Quantitative criteria for estimation of natural and artificial ecosystems functioning.

Using biotic turnover of substances in trophic chains, natural and artificial ecosystems are similar in functioning, but different in structure. It is necessary to have quantitative criteria to evaluate the efficiency of artificial ecosystems (AES). These criteria are dependent on the specific objectives for which the AES are designed. For example, if AES is considered for use in space, important criteria are efficiency in use of mass, power, volume (size) and human labor and reliability. Another task involves the determination of quantitative criteria for the functioning of natural ecosystems. To solve the problem, it is fruitful to use a hierarchical approach suitable for both individual links and the ecosystem as a whole. Energy flux criteria (principles) were developed to estimate the functional activities of biosystems at the population, community and ecosystem levels. A major feature of ecosystems as a whole is their biotic turnover of matter the rate of which is restricted by the lack of limiting substances. Obviously, the most generalized criterion is to take into account the energy flux used by the biosystem and the quantity of limiting substance included in its turnover. The use of energy flux by ecosystem, E(USED)--is determined from the photoassimilation of CO2 by plants (per time unit). It can be approximately estimated as the net primary production of photosynthesis (NPP). So, the ratio of CO2 photoassimilation rate (sometimes, measured as NPP) to the total mass of limiting substrate can serve as a main universal criterion (MUC). This MUC characterizes the specific cycling rate of limiting chemical elements in the system and effectiveness of every ecosystem including the global Biosphere. Comparative analysis and elaboration of quantitative criteria for estimation of natural and artificial ecosystems activities is of high importance both for theoretical considerations and for real applications.     

Principles of biological adaptation of organisms in artificial ecosystems to changes of environmental factors.

Studying material transformations and biotic cycling in artificial ecosystems (AES), we need to know the principles of biological adaptation of active organisms to change in the environment. Microorganisms in AES for water purification are the most active transforming organisms and consumers of the organic substances contained in wastes. Utilization of organic substances is directly connected with the energy fluxes used by AES. According to energy criteria, the energy fluxes used by a biological system tend to reach maximum values under stable conditions. Unutilized substrate concentration decreases as a result of biological adaptations. After a dramatic change in environmental factors, for example, after a sharp increase in the flow rate of organic substances, the biological system is not able to react quickly. The concentration of unutilized substrate increases and the energy flux used by the biological system decreases. The structure of the microbial community also changes, with a decrease in biological diversity. The efficiency of energy use by simple terrestrial ecosystems depends on the energetic intensity and interactions between plants and rhizospheric microorganisms.     

Experimental and mathematical model of the interactions in the mixed culture of links in the “producer–consumer” cycle

The paper presents a experimental and mathematical model of interactions between invertebrates (the ciliates Paramecium caudatum and the rotifers Brachionus plicatilis) in the “producer–consumer” aquatic biotic cycle with spatially separated components. The model describes the dynamics of the mixed culture of ciliates and rotifers in the “consumer” component, feeding on the mixed algal culture of the “producer” component. It has been found that metabolites of the algae Scenedesmus produce an adverse effect on the reproduction of the ciliates P. caudatum. Taking into account this effect, the results of investigation of the mathematical model were in qualitative agreement with the experimental results. In the “producer–consumer” biotic cycle it was shown that coexistence is impossible in the mixed culture of invertebrates of the “consumer” component. The ciliates P. caudatum are driven out by the rotifers B. plicatilis.     

The water cycle in closed ecological systems: Perspectives from the Biosphere 2 and Laboratory Biosphere systems

To achieve sustainable, healthy closed ecological systems requires solutions to challenges of closing the water cycle – recycling wastewater/irrigation water/soil medium leachate and evaporated water and supplying water of required quality as needed for different needs within the facility. Engineering Biosphere 2, the first multi-biome closed ecological system within a total airtight footprint of 12,700 m² with a combined volume of 200,000 m³ with a total water capacity of some 6 × 10⁶ L of water was especially challenging because it included human inhabitants, their agricultural and technical systems, as well as five analogue ecosystems ranging from rainforest to desert, freshwater ecologies to saltwater systems like mangrove and mini-ocean coral reef ecosystems. By contrast, the Laboratory Biosphere – a small (40 m³ volume) soil-based plant growth facility with a footprint of 15 m² – is a very simplified system, but with similar challenges re salinity management and provision of water quality suitable for plant growth. In Biosphere 2, water needs included supplying potable water for people and domestic animals, irrigation water for a wide variety of food crops, and recycling and recovering soil nutrients from wastewater. In the wilderness biomes, providing adequately low salinity freshwater terrestrial ecosystems and maintaining appropriate salinity and pH in aquatic/marine ecosystems were challenges. The largest reservoirs in Biosphere 2 were the ocean/marsh with some 4 × 10⁶ L, soil with 1 to 2 × 10⁶ l, primary storage tank with 0 to 8 × 10⁵ L and storage tanks for condensate and soil leachate collection and mixing tanks with a capacity of 1.6 × 10⁵ L to supply irrigation for farm and wilderness ecosystems. Other reservoirs were far smaller – humidity in the atmosphere (2 × 10³ L), streams in the rainforest and savannah, and seasonal pools in the desert were orders of magnitude smaller (8 × 10⁴ L). Key technologies included condensation from humidity in the air handlers and from the glass space frame to produce high quality freshwater, wastewater treatment with constructed wetlands and desalination through reverse osmosis and flash evaporation were key to recycling water with appropriate quality throughout the Biosphere 2 facility. Wastewater from all human uses and the domestic animals in Biosphere 2 was treated and recycled through a series of constructed wetlands, which had hydraulic loading of 0.9–1.1 m³ day⁻¹ (240–290 gal d⁻¹). Plant production in the wetland treatment system produced 1210 kg dry weight of emergent and floating aquatic plant wetland which was used as fodder for the domestic animals while remaining nutrients/water was reused as part of the agricultural irrigation supply. There were pools of water with recycling times of days to weeks and others with far longer cycling times within Biosphere 2. By contrast, the Laboratory Biosphere with a total water reservoir of less than 500 L has far quicker cycling rapidity: for example, atmospheric residence time for water vapor was 5–20 min in the Laboratory Biosphere vs. 1–4 h in Biosphere 2, as compared with 9 days in the Earth’s biosphere. Just as in Biosphere 2, humidity in the Laboratory Biosphere amounts to a very small reservoir of water. The amount of water passing through the air in the course of a 12-h operational day is two orders of magnitude greater than the amount stored in the air. Thus, evaporation and condensation collection are vital parts of the recycle system just as in Biosphere 2. The water cycle and sustainable water recycling in closed ecological systems presents problems requiring further research – such as how to control buildup of salinity in materially closed ecosystems and effective ways to retain nutrients in optimal quantity and useable form for plant growth. These issues are common to all closed ecological systems of whatever size, including planet Earth’s biosphere and are relevant to a global environment facing increasing water shortages while maintaining water quality for human and ecosystem health. Modular biospheres offer a test bed where technical methods of resolving these problems can be tested for feasibility.     

Simultaneous investigation of magnetospheric plasma and spacecraft charging

The results of magnetospheric plasma fluxes measurement in geosynchronous orbit are presented. The measurements were done onboard the geosynchronous Russian “Electro” spacecraft (SC) in 1995–1997 years. The diurnal variations of the plasma density and temperature were observed at various levels of geomagnetic activity.     

Energetic neutral atom (ENA) and charged particle periodicities in Saturn’s magnetosphere

The Magnetospheric Imaging Instrument (MIMI) on the Cassini spacecraft has observed energetic neutral atoms (ENA) and charged particles at Saturn from mid-2004 to the present. The particles often but not always reveal striking periodic behavior that seems to depend on the type of particle and spacecraft location. When subjected to a Lomb periodogram analysis, energetic electrons (>150 keV) exhibited strong frequency peaks near 10.80 h (the nominal or “base” period of Saturn kilometric radiation) during 2006–2008, but essentially no periodicity during 2005. The electron periodograms also show pronounced “double” frequency peaks in 2007 and 2008. Energetic protons (3–26 keV) show strong peaks near the same period for 2005–2007, but none for 2008. Oxygen ions at the same energies display strong peaks for 2005 and 2006, but not for 2007 and 2008. By projecting the ENA images onto Saturn’s equatorial plane or onto a plane perpendicular to the equatorial plane and then summing the data in the appropriate dimension, “strip” images can be constructed from which a time history can be derived. These time histories of ENA emissions are also subjected to a Lomb periodogram analyses. The energetic hydrogen neutrals (20–50 keV) exhibited periodic behavior only during 2007, while energetic oxygen neutrals (64–144 keV) displayed a strong SKR-like period in 2005 and 2006 but not for 2007 or 2008. Some of this behavior may be due to changing spacecraft aspect relative to the ENA emissions, and some of it may be real. This periodic behavior may be consistent with a rotating anomaly that “flashes” brightly in the midnight-to-dawn sector once per 10.8 h, with the flash parameters depending on particle species and energy.     

“Oblique” Bernstein modes in solar preflare plasma: Generation of second harmonics

Process of second harmonics generation due to development of corresponding instability has been investigated for pure electron weakly oblique Bernstein mode. This mode was supposed to be modified by taking into account the influence of pair Coulomb collisions and weak large-scale electric field in flare loop. Investigated area was located near the loop foot-point in the “lower–middle” chromosphere of active region. It has been shown, that for the Fontenla–Avrett–Loeser model of solar atmosphere the investigated process of second harmonics generation starts at the extremely low threshold values of subdreicer electric field, well before the beginning of “preheating” phase of flare process.     

Cellular communication and “non-targeted effects”: Modelling approaches

During the last decade, a large number of experimental studies on the so-called “non-targeted effects”, in particular bystander effects, outlined that cellular communication plays a significant role in the pathways leading to radiobiological damage. Although it is known that two main types of cellular communication (i.e. via gap junctions and/or molecular messengers diffusing in the extra-cellular environment, such as cytokines, NO etc.) play a major role, it is of utmost importance to better understand the underlying mechanisms, and how such mechanisms can be modulated by ionizing radiation. Though the “final” goal is of course to elucidate the in vivo scenario, in the meanwhile also in vitro studies can provide useful insights. In the present paper we will discuss key issues on the mechanisms underlying non-targeted effects and cell communication, for which theoretical models and simulation codes can be of great help. In this framework, we will present in detail three literature models, as well as an approach under development at the University of Pavia. More specifically, we will first focus on a version of the “State-Vector Model” including bystander-induced apoptosis of initiated cells, which was successfully fitted to in vitro data on neoplastic transformation supporting the hypothesis of a protective bystander effect mediated by apoptosis. The second analyzed model, focusing on the kinetics of bystander effects in 3D tissues, was successfully fitted to data on bystander damage in an artificial 3D skin system, indicating a signal range of the order of 0.7–1 mm. A third model for bystander effect, taking into account of spatial location, cell killing and repopulation, showed dose–response curves increasing approximately linearly at low dose rates but quickly flattening out for higher dose rates, also predicting an effect augmentation following dose fractionation. Concerning the Pavia approach, which can model the release, diffusion and depletion/degradation of candidate signals (e.g. cytokines) travelling in the extra-cellular environment, the good agreement with ad hoc experimental data obtained in our laboratory validated the adopted approach, which in the future can be applied also to other candidate signals.     

“Modular Biospheres” – New testbed platforms for public environmental education and research

This paper will review the potential of a relatively new type of testbed platform for environmental education and research because of the unique advantages resulting from their material closure and separation from the outside environment. These facilities which we term “modular biospheres”, have emerged from research centered on space life support research but offer a wider range of application. Examples of this type of facility include the Bios-3 facility in Russia, the Japanese CEEF (Closed Ecological Experiment Facility), the NASA Kennedy Space Center Breadboard facility, the Biosphere 2 Test Module and the Laboratory Biosphere. Modular biosphere facilities offer unique research and public real-time science education opportunities. Ecosystem behavior can be studied since initial state conditions can be precisely specified and tracked over different ranges of time. With material closure (apart from very small air exchange rate which can be determined), biogeochemical cycles between soil and soil microorganisms, water, plants, and atmosphere can be studied in detail. Such studies offer a major advance from studies conducted with phytotrons which because of their small size, limit the number of organisms to a very small number, and which crucially do not have a high degree of atmospheric, water and overall material closure. Modular biospheres take advantage of the unique properties of closure, as representing a distinct system “metabolism” and therefore are essentially a “mini-world”. Though relatively large in comparison with most phytotrons and ecological microcosms, which are now standard research and educational tools, modular biospheres are small enough that they can be economically reconfigured to reflect a changing research agenda. Some design elements include lighting via electric lights and/or sunlight, hydroponic or soil substrate for plants, opaque or glazed structures, and variable volume chambers or other methods to handle atmospheric pressure differences between the facility and the outside environment.     

Analysis of data of “Clementine” and “KAGUYA” missions and “ULCN” and “KSC-1162” catalogues

In this paper an analysis of data coordinate systems from selenographic catalogues and space missions was carried out. The lunar macrorelief models were made on basis of the software package ASNI USTU using method of the spherical harmonic expansion. These models accurately describe the global features of the lunar figure. To construct these models the following sources of topographic information were used: “Clementine” and “KАGUYА” (Selena, Japan mission) missions, “KSC-1162” (Kazan selenocentric catalogue), “Kiev” (selenodesic catalogue), “SAI” (Chuikova (1975)), “Bills, Ferrari”, “ULCN” (The Unified Lunar Control Network 2005). Direct comparison hypsometric information “KSС-1162” catalogue data with “Clementine” mission was carried out. These researches confirmed a good agreement of the hypsometric information of compared systems. The normalized coefficients were obtained on basis of the hypsometric information expansion for eight sources. The displacement of the lunar center of mass (LCM) relatively to the lunar center of figure (LCF) was obtained by using topographic data selenodetical catalogues and space missions.     

Integration of lessons from recent research for “Earth to Mars” life support systems

Development of reliable and robust strategies for long-term life support for planetary exploration must be built from real-time experimentation to verify and improve system components. Also critical is incorporating a range of viable options to handle potential short-term life system imbalances. This paper revisits some of the conceptual framework for a Mars base prototype which has been developed by the authors along with others previously advanced (“Mars on Earth^®”) in the light of three years of experimentation in the Laboratory Biosphere, further investigation of system alternatives and the advent of other innovative engineering and agri-ecosystem approaches. Several experiments with candidate space agriculture crops have demonstrated the higher productivity possible with elevated light levels and improved environmental controls. For example, crops of sweet potatoes exceeded original Mars base prototype projections by an average of 46% (53% for best crop) ultradwarf (Apogee) wheat by 9% (23% for best crop), pinto bean by 13% (31% for best crop). These production levels, although they may be increased with further optimization of lighting regimes, environmental parameters, crop density etc. offer evidence that a soil-based system can be as productive as the hydroponic systems which have dominated space life support scenarios and research. But soil also offers distinct advantages: the capability to be created on the Moon or Mars using in situ space resources, reduces long-term reliance on consumables and imported resources, and more readily recycling and incorporating crew and crop waste products. In addition, a living soil contains a complex microbial ecosystem which helps prevent the buildup of trace gases or compounds, and thus assist with air and water purification. The atmospheric dynamics of these crops were studied in the Laboratory Biosphere adding to the database necessary for managing the mixed stands of crops essential for supplying a nutritionally adequate diet in space. This paper explores some of the challenges of small bioregenerative life support: air-sealing and facility architecture/design, balance of short-term variations of carbon dioxide and oxygen through staggered plantings, options for additional atmospheric buffers and sinks, lighting/energy efficiency engineering, crop and waste product recycling approaches, and human factor considerations in the design and operation of a Mars base. An “Earth to Mars” project, forging the ability to live sustainably in space (as on Earth) requires continued research and testing of these components and integrated subsystems; and developing a step-by-step learning process.     

December 2006 solar extreme events and their influence on the near-Earth space environment: “Universitetskiy-Tatiana” satellite observations

The Russian microsatellite “Universitetskiy-Tatiana” was launched on Jan. 20, 2005 and was both a scientific and educational mission. Its two main aims were declared as: (1) monitoring of the energetic particles dynamics in the near-Earth space environment after solar events and during quiet times, (2) educational activities based on experimental data obtained from the spacecraft. In this paper observations acquired during Dec. 5–16, 2006, known as “Solar Extreme Events 2006”, were analyzed. The “Universitetskiy-Tatiana” microsatellite orbit permits one to measure both solar energetic particle dynamics, variations of the boundary of solar particle penetration, as well as relativistic and sub-relativistic electrons of the Earth’s outer radiation belt during and after magnetic storms. Both relativistic electrons of the Earth’s outer radiation and solar energetic particles are an important source of radiation damage in near-Earth space. Therefore, the presented experimental results demonstrate the successful application of a small educational spacecraft both for scientific and educational programs.     

Topology of the high latitude magnetosphere during large magnetic storms and the main mechanisms of relativistic electron acceleration

One of the main endeavors of the “Space Weather” program is the prediction of the appearance of very large fluxes of relativistic electrons with energies larger than 1 MeV, because they represent a serious potential hazard for satellite missions. Large fluxes of relativistic electrons are formed in the outer radiation belt during the recovery phase of some storms. The formation of large fluxes is connected to a balance between the acceleration and loss processes. A two-step acceleration process is ordinarily analyzed. A “Seed” population with energies ∼hundreds of keV appeared during expansion phase of magnetospheric substorm. A “Seed” population is additionally accelerated obtaining relativistic energies by some other process. Several acceleration mechanisms have been proposed for the explanation of the electron acceleration, including radial diffusion and internal acceleration by wave-particle interactions. Nevertheless, none of them takes into account great changes of magnetospheric topology during a magnetic storm. Such changes are mainly connected with asymmetric and symmetric ring current development. We analyze the changes of magnetospheric topology during magnetic storms. We show that a change of the magnetospheric magnetic field can be the important factor determining the acceleration of relativistic electrons.     

Spiral eddies in the Baltic,Black and Caspian seas as seen by satellite radar data

Despite spiral eddies were first seen on the sea surface more than 40 years ago, there is still a lot of uncertainty concerning these eddies. The present paper is aimed to provide the comprehensive results on the occurrence and statistics of small-scale eddies in the three inner seas (the Baltic, Black and Caspian) using satellite synthetic aperture radar (SAR) images. The dataset used includes over 2000 medium resolution Envisat ASAR and ERS-2 SAR images obtained in 2009–2010 in the different parts of the seas mentioned. As a result of the analysis performed ∼14,000 vortical structures were detected. 71% of them were visualized due to surfactant films (“black” eddies), while 29% due to wave/current interactions (“white” eddies). Practically all the eddies detected were cyclonically rotating. Their diameter was within 1–20 km. Characteristic size of the “black” eddies in all the basins was discovered to be less than that of the “white” eddies. Characteristic eddy size for the Baltic, Black and Caspian seas proved to be strictly proportional to the values of the baroclinic Rossby radius of deformation typical for these basins. The “black” eddies did not demonstrate a significant connection with the basin- and meso-scale surface circulation of the seas. Most of the “white” eddies detected were attributed to the zones with the most intense drift currents, i.e. those along the western boundaries and (in the Baltic Sea only) in the elongated parts of the basin.     

Observation bio-effect of SW-global solar radiation in Ilorin in the tropics

The annual variation of global (total) solar radiation measured over four (4) years (1995–1998) at Ilorin (8°32′N, 4°34′E) was studied and the bio-effects of the variation is herein reported. The weekly averages of the solar radiation flux were obtained and plotted. The profile of the weekly averages of the insolation shows two notable “wells” corresponding to the harmattan dry season and the rainy season. It was deduced that the duration of the seasons was directly proportional to the sizes of the “wells”, while the depths of the “wells” correspond to the severity of the causes of the “wells”. The fall and rise rates of the depth of the edges of the wells are inversely proportional to the ecological effects of the variation of the radiation. The rates of decrease and increase of the radiation flux affect the depths of the “wells” and were found to be rapid and shallow for the “Harmattan Well”, and gentle and deep for the “Rain Well”. The width (duration), rate of change and depth of the “Harmattan Well” bring about stress for both animals and plants.     

Old tail lobes effect on the solar-wind – Magnetosphere energy transport for the 27 August 2001 substorm

The magnetic flux of tail lobes Ψ is divided in two parts of comparable values Ψ₁ and Ψ₀₂, with the first that appears during substorm and the second, observed before substorm start. The first was named “new magnetic flux”, the second – “old magnetic flux”. The first, Ψ₁, is known to play a definitive role in the energy transport from the solar wind into the magnetosphere-ionosphere-atmosphere system, but the role of Ψ₀₂ in this transport is not well known. From the 27 August 2001 substorm data we study the involvement in the above transport process of the old flux Ψ₀₂. This involvement is observed in the polar cap (PC) area, which existed prior to the substorm and is called respectively “the old PC”. In this study, as distinct from earlier works, we use the balance equation of the energy stored in magnetosphere and energy consumed. Activation of the old PC magnetic flux Ψ₀₂ was found to increase the energy input by ∼85% in the event under consideration.     

GOCE orbit analysis: Long-wavelength gravity field determination using the acceleration approach

The restricted sensitivity of the Gravity field and steady-state Ocean Circulation Explorer (GOCE) gradiometer instrument requires satellite gravity gradiometry to be supplemented by orbit analysis in order to resolve long-wavelength features of the geopotential. For the hitherto published releases of the GOCE time-wise (TIM) and GOCE space-wise gravity field series—two of the official ESA products—the energy conservation method has been adopted to exploit GPS-based satellite-to-satellite tracking information. On the other hand, gravity field recovery from data collected by the CHAllenging Mini-satellite Payload (CHAMP) satellite showed the energy conservation principle to be a sub-optimal choice. For this reason, we propose to estimate the low-frequency part of the gravity field by the point-wise solution of Newton’s equation of motion, also known as the acceleration approach. This approach balances the gravitational vector with satellite accelerations, and hence is characterized by (second-order) numerical differentiation of the kinematic orbit. In order to apply the method to GOCE, we present tailored processing strategies with regard to low-pass filtering, variance–covariance information handling, and robust parameter estimation. By comparison of our GIWF solutions (initials GI for “Geodätisches Institut” and IWF for “Institut für WeltraumForschung”) and the GOCE-TIM estimates with a state-of-the-art gravity field solution derived from GRACE (Gravity Recovery And Climate Experiment), we conclude that the acceleration approach is better suited for GOCE-only gravity field determination as opposed to the energy conservation method.     

The electron formations under the radiation belts at L-shells 1.2–1.9

We continue to analyze the distribution of electron fluxes with energy 30–500 keV under the radiation belts at low and middle latitudes (L = 1.2–1.9) using experimental data obtained onboard ACTIVE satellite. Special attention is given to altitudinal distribution of electron fluxes and detailed analysis of these electron formations. We observe three main regions of electron flux registration that seem to exist constantly under the radiation belts. These regions are: magneto-conjugated to SAA region (in the north hemisphere), local zone of low intense electron flux accumulation to the west of SAA, and extensive region in the north hemisphere to the east. The analysis of experimental data obtained from ACTIVE satellite (orbit height 500–2500 km) shows that electron fluxes are registered in the wide altitude range up to 1100 km. It is shown that these formations have complicated initial structure with two regions of flux maximums: at L = 1.3 and L = 1.6–1.8. We compare particle data with low frequency (LF) data (ARIEL-4 satellite) and high frequency (HF) data (CORONAS-I satellite). Also we discuss the possible mechanisms of the appearance of these formations under the radiation belts.     

Selection of candidate salad vegetables for controlled ecological life support system

Higher plants, as one of the essential biological components of CELSS, can supply food, oxygen and water for human crews during future long-duration space missions and Lunar/Mars habitats. In order to select suitable leaf vegetable varieties for our CELSS Experimental Facility (CEF), five varieties of lettuce (“Nenlvnaiyou”, “Dasusheng”, “Naichoutai”, “Dongfangkaixuan” and “Siji”), two of spinach (“Daye” and “Quanneng”), one of rape (“Jingyou No. 1”) and one of common sowthistle were grown and compared on the basis of edible biomass, and nutrient content. In addition, two series of experiments were conducted to study single leaf photosynthetic rates and transpiration rates at 30 days after planting, one which used various concentrations of CO₂ (500, 1000, 1500 and 2000 μmol mol⁻¹) and another which used various light intensities (100, 300, 500 and 700 μmol m⁻² s⁻¹). Results showed that lettuce cvs. “Nenlvnaiyou”, “Siji” and “Dasusheng” produced higher yields of edible biomass; common sowthisle would be a good source of β-carotene for the diet. Based on the collective findings, we selected three varieties of lettuce (“Nenlvnaiyou”, “Dasusheng” and “Siji”) and one of common sowthistle as the candidate crops for further research in our CEF. In addition, elevated CO₂ concentration increased the rates of photosynthesis and transpiration, and elevated light intensity increased the rate of photosynthesis for these varieties. These results can be useful for determining optimal conditions for controlling CO₂ and water fluxes between the crops and the overall CELSS.     

Closed Artificial ecosystems as a means of ecosystem studies for Earth and space needs.

Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together "holistic" and "merological" approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of "ecosystems health" concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. Grant numbers: N 99-04-96017, N25.