Response of photosynthetic systems to salinity stress in the desert cyanobacterium Scytonema javanicum

The present study investigated the physiological and biochemical characteristics of Scytonema javanicum, a pioneer species isolated from desert biological crusts, under salinity stress. Pigment analysis showed that salinity decreased chlorophyll a and phycocyanin content, while low salinity increased carotenoid concentration and high salinity decreased carotenoid concentration. Salinity also inhibited CO₂ assimilation rate and photosynthetic oxygen evolution in this cyanobacterium. Chlorophyll a fluorescence transient parameters (φPo, φEo, ψO, RC/ABS, RC/CS, PI_ABS, and PI_CS) were decreased under salt stress, while dVo/dto(Mo), Vj and φDo were increased. The decrease of ETRmax and Yield and the change of chlorophyll a fluorescence transients showed that salt stress had an important influence on photosynthesis. These results indicated that the effects of salinity stress on photosynthesis in S. javanicum may depend on the inhibition of electron transport and the inactivation of the reaction centers, but this inhibition may occur in the electron transport pathway at the PSII donor and acceptor sites.     

Antioxidant capacity reduced in scallions grown under elevated CO2 independent of assayed light intensity

Long-duration manned space missions mandate the development of a sustainable life support system and effective countermeasures against damaging space radiation. To mitigate the risk of inevitable exposure to space radiation, cultivation of fresh fruits and vegetables rich in antioxidants is an attractive alternative to pharmacological agents. However it has yet to be established whether antioxidant properties of crops can be preserved or enhanced in a space environment where environmental conditions differ from that which plants have acclimated to on earth. Scallion (Allium fistulosum) rich in antioxidant vitamins C and A, and flavonoids was used as a model plant to study the impact of a range of CO₂ concentrations and light intensities that are likely encountered in a space habitat on food quality traits. Scallions were hydroponically grown in controlled environmental chambers under a combination of 3 CO₂ concentrations of 400, 1200 and 4000 μmol mol⁻¹ and 3 light intensity levels of 150, 300, 450 μmol m⁻² s⁻¹. Total antioxidant activity (TAA) of scallion extracts was determined using a radical cation scavenging assay. Both elevated CO₂ and increasing light intensity enhanced biomass accumulation, but effects on TAA (based on dry weight) differed. TAA was reduced for plants grown under elevated CO₂, but remained unchanged with increases in light intensity. Elevated CO₂ stimulated greater biomass production than antioxidants, while an increase in photosynthetic photo flux promoted the synthesis of antioxidant compounds at a rate similar to that of biomass. Consequently light is a more effective stimulus than CO₂ for antioxidant production.     

Variability of foE in the equatorial ionosphere with solar activity

This research examined the variability of f_oE in the equatorial ionosphere with solar activity within the equatorial ionospheric anomaly region. Ionosonde data recorded at Ouagadougou (lat. 12.4°N, long. 1.5°W and magnetic dip 1.43°N) were engaged to study the transient variations of the critical frequency of the E-layer (f_oE) and its dependence on solar activity. The study revealed that f_oE increases with the increase in solar intensity of the sun. The variability of the f_oE decreases with increases in the solar activity. The maximum value of the f_oE is at local noon when the ionosphere is stable; the variability at this local time is minimal. The minimum value of the f_oE is at sunrise and sunset, at this period on local time the equatorial ionosphere recorded its maxima variability. Irrespective of the degree of solar activity, f_oE is observed to be maximum in June solstice, followed by the equinoxes and minimum in December solstice. Equinoctial asymmetry occurred in the variation of the relative standard deviation of f_oE with maximum in September/March equinox for low/high solar activity.     

Comparing IRI and a regional model with ionosonde measurements in Pakistan

We have used the technique of expansion in Empirical Orthogonal Functions (EOFs) to develop regional models of the critical frequencies of E and F₂ layers (f_oE, f_oF₂), peak height (h_mF₂), and semi-thickness of F₂ layer (Y_mF₂) over Pakistan. In the present study levels of solar activity specified by Smoothed Sunspot Number (R) from 10 to 200 are taken into account. The magnetic dip angle for the model ranges from 30° to 60°. We have compared the regional model and the International Reference Ionosphere (IRI) with measurements of three ionosondes in Pakistan. The model parameters f_oE and f_oF₂ are found overall comparable to the observed hourly median values during daytime at Karachi (geographic latitude = 24.95°N, longitude = 67.13°E, magnetic inclination = 37°), Multan (30.18°N, 71.48°E, 45°) and Islamabad (33.75°N, 73.13°E, 51.5°) during the years 1988, 1996 and 2000. For h_mF₂ the computed values by regional and IRI model for the year 1995 are found close to each other. However, for Y_mF₂the results are better during daytime as compared to nighttime.     

Rotational constants of linear and/or bent Cn+1H and CnN(n = 1–6): A DFT study

The geometries, dipole moments, and rotational constants for the linear and/or bent cations, C_n+1H⁺ and C_nN⁺(n = 1–6), were studied by the B3LYP method with the modest basis sets. For C_nH⁺(n = odd; 3, 5, 7) and C_nN⁺(n = even; 2, 4, 6), the theoretical rotational constants (B_es) of closed-shell singlet C₃H⁺, C₅H⁺, C₇H⁺, CCN⁺, C₄N⁺, and C₆N⁺ were calculated to be about 11,244, 2420, 885.2, 11,970, 2439, and 880.8 MHz, respectively. By contrast, the triplets are stable than the corresponding singlets for C_nH⁺(n = odd; 2, 4, 6) and C_nN⁺(n = even; 3, 5) except CN⁺.     

Precursory signature of several major earthquakes studied using 40 kHz low frequency signal

To investigate the precursory signature of earthquakes on low frequency (LF) signal propagation, six earthquakes, having magnitude greater than equal to 6.5 and depth less than equal to 30 km, are being studied. The base line level of 40 kHz signal, transmitted from JJY station, Japan, is analysed with respect to V_d statistical parameter. Results show that the V_d parameter values starts fluctuating from its ambient levels before and during the days of the earthquakes, with significant variation starting 1–3 days prior to the earthquake concerned. This present study is an approach for identifying the precursory signatures of earthquakes on LF signal propagation using a new methodology with V_d parameter.     

Gas exchange rates of potato stands for bioregenerative life support

Plants can provide a means for removing carbon dioxide (CO₂) while generating oxygen (O₂) and clean water for life support systems in space. To study this, 20 m² stands of potato (Solanum tuberosum L.) plants were grown in a large (113 m³ vol.), atmospherically closed chamber. Photosynthetic uptake of CO₂ by the stands was detected about 10 DAP (days after planting), after which photosynthetic rates rose rapidly as stand ground cover and total light interception increased. Photosynthetic rates peaked ca. 50 DAP near 45 μmol CO₂ m⁻² s⁻¹ under 865 μmol m⁻² s⁻¹ PPF (average photosynthetic photon flux), and near 35 μmol CO₂ m⁻² s⁻¹ under 655 μmol m⁻² s⁻¹ PPF. Short term changes in PPF caused a linear response in stand photosynthetic rates up to 1100 μmol m⁻² s⁻¹ PPF, with a light compensation point of 185 μmol m⁻² s⁻¹ PPF. Comparisons of stand photosynthetic rates at different CO₂ concentrations showed a classic C₃ response, with saturation occurring near 1200 μmol mol⁻¹ CO₂ and compensation near 100 μmol mol⁻¹ CO₂. In one study, the photoperiod was changed from 12 h light/12 h dark to continuous light at 58 DAP. This caused a decrease in net photosynthetic rates within 48 h and eventual damage (scorching) of upper canopy leaves, suggesting the abrupt change stressed the plants and/or caused feedback effects on photosynthesis. Dark period (night) respiration rates increased during early growth as standing biomass increased and peaked near 9 μmol CO₂ m⁻² s⁻¹ ca. 50 DAP, after which rates declined gradually with age. Stand transpiration showed a rapid rise with canopy ground cover and peaked ca. 50 DAP near 8.9 L m⁻² d⁻¹ under 860 μmol m⁻² s⁻¹ PPF and near 6.3 L m⁻² d⁻¹ under 650 μmol m⁻² s⁻¹ PPF. Based on the best photosynthetic rates from these studies, approximately 25 m² of potato plants under continuous cultivation would be required to support the CO₂ removal and O₂ requirements for one person.     

Contribution of satellite laser ranging to combined gravity field models

In the framework of satellite-only gravity field modeling, satellite laser ranging (SLR) data is typically exploited to recover long-wavelength features. This contribution provides a detailed discussion of the SLR component of GOCO02S, the latest release of combined models within the GOCO series. Over a period of five years (January 2006 to December 2010), observations to LAGEOS-1, LAGEOS-2, Ajisai, Stella, and Starlette were analyzed. We conducted a series of closed-loop simulations and found that estimating monthly sets of spherical harmonic coefficients beyond degree five leads to exceedingly ill-posed normal equation systems. Therefore, we adopted degree five as the spectral resolution for real data analysis. We compared our monthly coefficient estimates of degree two with SLR and Gravity Recovery and Climate Experiment (GRACE) time series provided by the Center for Space Research (CSR) at Austin, Texas. Significant deviations in C₂₀ were noted between SLR and GRACE; the agreement is better for the non-zonal coefficients. Fitting sinusoids together with a linear trend to our C₂₀ time series yielded a rate of (−1.75 ± 0.6) × 10⁻¹¹/yr; this drift is equivalent to a geoid change from pole to equator of 0.35 ± 0.12 mm/yr or an apparent Greenland mass loss of 178.5 ± 61.2 km³/yr. The mean of all monthly solutions, averaged over the five-year period, served as input for the satellite-only model GOCO02S. The contribution of SLR to the combined gravity field model is highest for C₂₀, and hence is essential for the determination of the Earth’s oblateness.     

A different method to update monthly median hmF2 values

The height, h_mF2, and the electron density, N_mF2, of the F2 peak are key model parameters to characterize the actual state of the ionosphere. These parameters, or alternatively the propagation factor, M3000F2, and the critical frequency, f_oF2, of the F2 peak, which are related to h_mF2 and N_mF2, are used to anchor the electron density vertical profile computed with different models such as the International Reference Ionosphere ( Bilitza, 2002), as well as for radio propagation forecast purposes. Long time series of these parameters only exist in an inhomogeneous distribution of points over the surface of Earth, where dedicated instruments (typically ionosondes) have been working for many years. A commonly used procedure for representing median values of the aforementioned parameters all over the globe is the one recommended by the ITU-R ( ITU-R, 1997). This procedure, known as the Jones and Gallet mapping technique, was based on ionosondes measurements gathered from 1954 to 1958 by a global network of around 150 ionospheric stations (  and ). Even though several decades have passed since the development of that innovative work, only few efforts have been dedicated to establish a new mapping technique for computing h_mF2 and N_mF2 median values at global scale or to improve the old method using the increased observational database. Therefore, in this work three different procedures to describe the daily and global behavior of the height of the F2 peak are presented. All of them represent a different and simplified method to estimate h_mF2 and are based on different mathematical expressions. The advantages and disadvantages of these three techniques are analyzed, leading to the conclusion that the recommended procedure to represent h_mF2 is best characterized by a Spherical Harmonics expansion of degree and order equal to 15, since the differences between the h_mF2 values obtained with the Jones and Gallet technique and those obtained using the abovementioned procedure are of only 1%.     

Tolerance of chufa (Cyperus esculentus L.) plants,representing the higher plant compartment in bioregenerative life support systems,to super-optimal air temperatures

Plants intended to be included in the photosynthesizing compartment of the bioregenerative life support system (BLSS) need to be studied in terms of both their production parameters under optimal conditions and their tolerance to stress factors that might be caused by emergency situations. The purpose of this study was to investigate tolerance of chufa (Cyperus esculentus L.) plants to the super-optimal air temperature of 45 ± 1 °C as dependent upon PAR (photosynthetically active radiation) intensity and the duration of the exposure to the stress factor. Chufa plants were grown hydroponically, on expanded clay, under artificial light. The nutrient solution was Knop’s mineral medium. Until the plants were 30 days old, they had been grown at 690 μmol m⁻² s⁻¹ PAR and air temperature 25 °C. Thirty-day-old plants were exposed to the temperature 45 °C for 6 h, 20 h, and 44 h at PAR intensities 690 μmol m⁻² s⁻¹ and 1150 μmol m⁻² s⁻¹. The exposure to the damaging air temperature for 44 h at 690 μmol m⁻² s⁻¹ PAR caused irreversible damage to PSA, resulting in leaf mortality. In chufa plants exposed to heat shock treatment at 690 μmol m⁻² s⁻¹ PAR for 6 h and 20 h, respiration exceeded photosynthesis, and CO₂ release in the light was recorded. Functional activity of photosynthetic apparatus, estimated from parameters of pulse-modulated chlorophyll fluorescence in Photosystem 2 (PS 2), decreased 40% to 50%. After the exposure to the stress factor was finished, functional activity of PSA recovered its initial values, and apparent photosynthesis (P_apparent) rate after a 20-h exposure to the stress factor was 2.6 times lower than before the elevation of the temperature. During the first hours of plant exposure to the temperature 45 °C at 1150 μmol m⁻² s⁻¹ PAR, respiration rate was higher than photosynthesis rate, but after 3–4 h of the exposure, photosynthetic processes exceeded oxidative ones and CO₂ absorption in the light was recorded. At the end of the 6-h exposure, P_apparent rate was close to that recorded prior to the exposure, and no significant changes were observed in the functional activity of PSA. At the end of the 20-h exposure, P_apparent rate was close to its initial value, but certain parameters of the functional activity of PSA decreased 25% vs. their initial values. During the repair period, the parameters of external gas exchange recovered their initial values, and parameters of pulse-modulated chlorophyll fluorescence were 20–30% higher than their initial values. Thus, exposure of chufa plants to the damaging temperature of the air for 20 h did not cause any irreversible damage to the photosynthetic apparatus of plants at either 690 μmol m⁻² s⁻¹ or 1150 μmol m⁻² s⁻¹ PAR, and higher PAR intensity during the heat shock treatment enhanced heat tolerance of the plants.     

Efficacy of oxygen-supplying capacity of Azolla in a controlled life support system

Azolla shows high growth and propagation rates, strong photosynthetic O₂-releasing ability and high nutritional value. It is suitable as a salad vegetable and can be cultured on a multi-layered wet bed. Hence, it possesses potential as a fresh vegetable, and to release O₂ and absorb CO₂ in a Controlled Ecological Life Support System in space. In this study, we investigated the O₂-providing characteristics of Azolla in a closed chamber under manned, controlled conditions to lay a foundation for use of Azolla as a biological component in ground simulation experiments for space applications. A closed test chamber, representing a Controlled Ecological Life Support System including an Azolla wet-culture device, was built to measure the changes in atmospheric O₂ and CO₂ concentrations inside the chamber in the presence of coexisting Azolla, fish and men. The amount of O₂ consumed by fish was 0.0805–0.0831 L kg⁻¹ h⁻¹ and the level of CO₂ emission was 0.0705–0.0736 L kg⁻¹ h⁻¹; O₂ consumption by the two trial volunteers was 19.71 L h⁻¹ and the volume of respiration-released CO₂ was 18.90 L h⁻¹. Under 7000–8000 Lx artificial light and Azolla wet-culture conditions, human and fish respiration and Azolla photosynthesis were complementary, thus the atmospheric O₂ and CO₂ concentrations inside chamber were maintained in equilibrium. The increase in atmospheric CO₂ concentration in the closed chamber enhanced the net photosynthesis efficiency of the Azolla colony. This study showed that Azolla has strong photosynthetic O₂-releasing ability, which equilibrates the O₂ and CO₂ concentrations inside the chamber in favor of human survival and verifies the potential of Azolla for space applications.     

Gamma ray bursts: Short vs. long

Short and long GRBs are thought to be two distinct classes based on their different duration and spectrum. Through the spectral analysis of two similarly selected samples of BATSE short and long GRBs, we show that short GRBs are harder than long events, confirming what found from the comparison of their hardness ratio. However, this spectral diversity seems to be due to a harder low energy spectral component of short GRBs, rather than a (slightly higher) peak energy. Interestingly short GRBs have a spectrum which is similar to the spectrum of the emission of the first 1–2 s of long events. We find evidence that short GRBs are inconsistent with the E_peak–E_iso correlation defined by long bursts while they follow the same E_peak–L_iso correlation of long GRBs. These results, coupled to the similar variability timescale of short events and the first seconds of long ones, suggest that a common (or similar) dissipation mechanism could operate in both classes. The difference in the duration would then be due mainly to the central engine lifetime.     

Radiosensitivity to high energy iron ions is influenced by heterozygosity for Atm, Rad9 and Brca1

Loss of function of DNA repair genes has been implicated in the development of many types of cancer. In the last several years, heterozygosity leading to haploinsufficiency for proteins involved in DNA repair was shown to play a role in genomic instability and carcinogenesis after DNA damage is induced, for example by ionizing radiation. Since the effect of heterozygosity for one gene is relatively small, we hypothesize that predisposition to cancer could be a result of the additive effect of heterozygosity for two or more genes critical to pathways that control DNA damage signaling, repair or apoptosis. We investigated the role of heterozygosity for Atm, Rad9 and Brca1 on cell oncogenic transformation and cell survival induced by 1 GeV/n⁵⁶Fe ions. Our results show that cells heterozygous for both Atm and Rad9 or Atm and Brca1 have high survival rates and are more sensitive to transformation by high energy iron ions when compared with wild-type controls or cells haploinsufficient for only one of these proteins. Since mutations or polymorphisms for similar genes exist in a small percentage of the human population, we have identified a radiosensitive sub-population. This finding has several implications. First, the existence of a radiosensitive sub-population may distort the shape of the dose–response relationship. Second, it would not be ethical to put exceptionally radiosensitive individuals into a setting where they may potentially be exposed to substantial doses of radiation.     

A study of L band scintillations during the initial phase of rising solar activity at an Indian low latitude station

The ionospheric scintillation and TEC (Total Electron Content) variations are studied using GPS (Global Positioning System) measurements at an Indian low latitude station Surat (21.16°N, 72.78°E; Geomagnetic: 12.90°N, 147.35°E), situated near the northern crest of the equatorial anomaly region. The results are presented for data collected during the initial phase of current rising solar activity (low to moderate solar activity) period between January 2009 and December 2011. The results show that within a total number of 656 night-time scintillation events, 340 events are observed with TEC depletions, Rate of change of TEC (ROT) fluctuations and enhancement of Rate of change of TEC Index (ROTI). A comparison of night-time scintillation events from the considered period reveal strong correlation amongst the duration of scintillation activity in S₄ index_, TEC depletion, ROT fluctuations and ROTI enhancement in the year 2011, followed by the year 2010 and least in 2009. The statistical analyses of scintillation activity with enhancement of ROTI also show that about 70–96% scintillation activity took place in equinox and winter months. Moreover, from a nocturnal variation in occurrence of scintillation with (S₄ ? 0.2) and enhancement of ROTI with (ROTI ? 0.5), a general trend of higher occurrence in pre-midnight hours of equinox and winter seasons is observed in both indices during the year 2011 and 2010, while no significant trend is observed in the year 2009. The results suggest the presence of F-region ionospheric irregularities with scale sizes of few kilometers and few hundred meters over Surat and are found to be influenced by solar and magnetic activity.     

Life under solar UV radiation in aquatic organisms.

Aquatic photosynthetic organisms are exposed to solar ultraviolet (UV) radiation while they harvest longer wavelength radiation for energetic reasons. Solar UV-B radiation (280-315 nm) affects motility and orientation in motile organisms and impairs photosynthesis in cyanobacteria, phytoplankton and macroalgae as measured by monitoring oxygen production or pulse amplitude modulated fluorescence analysis. Upon moderate UV stress most organisms respond by photoinhibition which is an active downregulation of the photosynthetic electron transport in photosystem II by degradation of UV-damaged D1 protein. Photoinhibition is readily reversible during recovery in shaded conditions. Excessive UV stress causes photodamage which is not easily reversible. Another major target is the DNA where UV-B mainly induces thymine dimers. Cyanobacteria, phytoplankton and macroalgae produce scytonemin, mycosporine-like amino acids and other UV-absorbing substances to protect themselves from short wavelength solar radiation.     

Study on O2 generation and CO2 absorption capability of four co-cultured salad plants in an enclosed system

The ability to generate O₂ and absorb CO₂ of several co-cultured vegetable plants in an enclosed system was studied to provide theoretical reference for the future man-plant integrated tests. Four kinds of salad plants (Lactuca sativa L. var. Dasusheng, Lactuca sativa L. var. Youmaicai, Gynura bicolor and Cichorium endivia L.) were grown in the CELSS Integration Test Platform (CITP). The environmental factors including O₂ and CO₂ concentration were continuously monitored on-line and the plant biomass was measured at the end of the test. The changing rules of O₂ and CO₂ concentration in the system were basically understood and it was found that the O₂ generated by the plants could satisfy the respiratory needs of 1.75 persons by calculation. It was also found that the plants could absorb the CO₂ breathed out by 2 persons when the light intensity was raised to 550 mmol m⁻² s⁻¹ PPF. The results showed that the co-cultured plants hold good compatibility and excellent O₂-generating and CO₂-absorbing capability. They could also supply some fresh edible vegetable for a 2-person crew.     

Symmetry,reduction and relative equilibria of a rigid body in the J2 problem

The J₂ problem is an important problem in celestial mechanics, orbital dynamics and orbital design of spacecraft, as non-spherical mass distribution of the celestial body is taken into account. In this paper, the J₂ problem is generalized to the motion of a rigid body in a J₂ gravitational field. The relative equilibria are studied by using geometric mechanics. A Poisson reduction process is carried out by means of the symmetry. Non-canonical Hamiltonian structure and equations of motion of the reduced system are obtained. The basic geometrical properties of the relative equilibria are given through some analyses on the equilibrium conditions. Then we restrict to the zeroth and second-order approximations of the gravitational potential. Under these approximations, the existence and detailed properties of the relative equilibria are investigated. The orbit–rotation coupling of the rigid body is discussed. It is found that under the second-order approximation, there exists a classical type of relative equilibria except when the rigid body is near the surface of the central body and the central body is very elongated. Another non-classical type of relative equilibria can exist when the central body is elongated enough and has a low average density. The non-classical type of relative equilibria in our paper is distinct from the non-Lagrangian relative equilibria in the spherically-simplified Full Two Body Problem, which cannot exist under the second-order approximation. Our results also extend the previous results on the classical type of relative equilibria in the spherically-simplified Full Two Body Problem by taking into account the oblateness of the primary body. The results on relative equilibria are useful for studies on the motion of many natural satellites, whose motion are close to the relative equilibria.     

Physical implications of discrepancy between summer and winter PC indices observed in the course of magnetospheric substorms

The PC index based on a statistically justified relationship between the polar cap magnetic activity and the interplanetary electric field E_KL has been derived as a value standardized for the E_KL intensity regardless of season, UT and hemisphere. As a result, the summer and winter PC indices are consistent with one another under ordinary conditions. Discrepancies between the summer and winter PC indices arising in the course of magnetospheric substorms are analyzed in this paper. It is argued that the channel of enhanced conductivity, formed in the auroral oval owing to intense auroral particle precipitation, strongly improves the conditions for closure of the Region 1 field-aligned currents in the winter dark polar region but only trivially affects the conditions of the Region 1 FAC closure in the summer sunlit ionosphere. Since the coefficients describing the relationship between E_KL and the polar cap magnetic activity were derived for statistically justified (i.e., mean) conditions, their application to such abnormal situation, as intense field-aligned currents in the winter dark polar region, leads to overestimation of the winter PC index. The summer and winter PC indices level off as soon as the intense auroral particle precipitation terminates and the auroral ionosphere in the winter and summer polar caps returns to the ordinary (statistically justified) state.     

Evolution of periodic orbits near the Lagrangian point L2

A study of the evolution of the periodic and the quasi-periodic orbits near the Lagrangian point L₂, which is located to the right of the smaller primary on the line joining the primaries and whose distance from the more massive primary is greater than the distance between the primaries, in the framework of restricted three-body problem for the Sun–Jupiter, Earth–Moon (relatively large mass ratio) and Saturn–Titan (relatively small mass ratio) systems is made. Two families of periodic orbits around the smaller primary are identified using the Poincaré surface of section method – family I (initially elliptical, gradually becomes egg-shaped with the increase in the Jacobi constant C and elongated towards the more massive primary) and family II (initially egg-shaped orbits elongated towards L₂ and gradually becomes elliptical with the increase in C). The family I in the Sun–Jupiter and Saturn–Titan systems contains two separatrix caused by third-order and fourth-order resonances, while the Earth–Moon system has only one separatrix which is caused by third-order resonances. Also in the Sun–Jupiter and the Saturn–Titan systems, family I merge with family II, around Jacobian constant 3.0393 and 3.0163, respectively, while in the Earth–Moon system, family II evolves separately from two different branches. The two branches merge at C = 3.184515. In the Earth–Moon system, the family II contains a separatrix due to third-order resonances which is absent in the other two systems.     

The effects of CO2 on growth and transpiration of radish (Raphanus sativus) in hypobaria

Plants grown on long-term space missions will likely be grown in low pressure environments (i.e., hypobaria). However, in hypobaria the transpiration rates of plants can increase and may result in wilting if the water is not readily replaced. It is possible to reduce transpiration by increasing the partial pressure of CO₂ (pCO₂), but the effects of pCO₂ at high levels (>120 Pa) on the growth and transpiration of plants in hypobaria are not known. Therefore, the effects of pCO₂ on the growth and transpiration of radish (Raphanus sativus var. Cherry Bomb II) in hypobaria were studied. The fresh weight (FW), leaf area, dry weight (DW), CO₂ assimilation rates (C_A), dark respiration rates (DR), and transpiration rates from 26 day-old radish plants that were grown for an additional seven days at different total pressures (33, 66 or 101 kPa) and pCO₂ (40 Pa, 100 Pa and 180 Pa) were measured. In general, the dry weight of plants increased with CO₂ enrichment and with lower total pressure. In limiting pCO₂ (40 Pa) conditions, the transpiration for plants grown at 33 kPa was approximately twice that of controls (101 kPa total pressure with 40 Pa pCO₂). Increasing the pCO₂ from 40 Pa to 180 Pa reduced the transpiration rates for plants grown in hypobaria and in standard atmospheric pressures. However, for plants grown in hypobaria and high pCO₂ (180 Pa) leaf damage was evident. Radish growth can be enhanced and transpiration reduced in hypobaria by enriching the gas phase with CO₂ although at high levels leaf damage may occur.