The effect of hypokinesia on lipid metabolism in adipose tissue

The increase of nonesterified fatty acid (NEFA) concentration in plasma was observed in rats subjected to hypokinesia for 1-60 days. In the period of recovery (7 and 21 days after 60 days immobilization) the content of NEFA returned to control values. The increase of fatty acid release from adipose tissue was observed in hypokinetic rats, however the stimulation of lipolysis by norepinephrine was lower in rats exposed to hypokinesis. The decrease of the binding capacity and a diminished number of beta-adrenergic receptors were found in animals after hypokinesia. The augmentation of the incorporation of glucose into lipids and the marked increase in the stimulation of lipogenesis by insulin were found in adipose tissue of rats subjected to long-term hypokinesia. These results showed an important effect of hypokinesia on lipid mobilization, on lipogenesis and on the processes of hormone regulation in adipose tissue.     

Changes of insulin effect on lipogenesis and insulin binding receptors during hypokinesia.

The effect of hypokinesia on insulin action and insulin binding to specific receptors in fat cells was studied. Male Wistar rats were exposed to hypokinesia in special adjustable plastic cages for 1, 7, 21 and 60 days, and the stimulatory effect of insulin (10 and 100 mU) on the incorporation of radiocarbon labelled glucose into lipids of fat tissue and the binding of insulin to receptors of isolated adipocytes was estimated. The stimulation of lipogenesis by insulin was slightly diminished after hypokinesia for 1 day, however, an important increase of insulin action was found in rats exposed to hypokinesia for 60 days. The decrease of insulin binding capacity of the number of binding sites per cell and of the insulin receptor density was found after 1 day of hypokinesia. In rats exposed to hypokinesia for 60 days, in agreement with the higher stimulatory affect of insulin, an increase of insulin receptor density was observed. These results showed that hypokinesia has an important influence on stimulatory action of insulin and on insulin receptors in adipocytes.     

A microbial oasis in the hypersaline Atacama subsurface discovered by a life detector chip: implications for the search for life on Mars

The Atacama Desert has long been considered a good Mars analogue for testing instrumentation for planetary exploration, but very few data (if any) have been reported about the geomicrobiology of its salt-rich subsurface. We performed a Mars analogue drilling campaign next to the Salar Grande (Atacama, Chile) in July 2009, and several cores and powder samples from up to 5?m deep were analyzed in situ with LDChip300 (a Life Detector Chip containing 300 antibodies). Here, we show the discovery of a hypersaline subsurface microbial habitat associated with halite-, nitrate-, and perchlorate-containing salts at 2?m deep. LDChip300 detected bacteria, archaea, and other biological material (DNA, exopolysaccharides, some peptides) from the analysis of less than 0.5?g of ground core sample. The results were supported by oligonucleotide microarray hybridization in the field and finally confirmed by molecular phylogenetic analysis and direct visualization of microbial cells bound to halite crystals in the laboratory. Geochemical analyses revealed a habitat with abundant hygroscopic salts like halite (up to 260?g kg(-1)) and perchlorate (41.13?μg g(-1) maximum), which allow deliquescence events at low relative humidity. Thin liquid water films would permit microbes to proliferate by using detected organic acids like acetate (19.14?μg g(-1)) or formate (76.06?μg g(-1)) as electron donors, and sulfate (15875?μg g(-1)), nitrate (13490?μg g(-1)), or perchlorate as acceptors. Our results correlate with the discovery of similar hygroscopic salts and possible deliquescence processes on Mars, and open new search strategies for subsurface martian biota. The performance demonstrated by our LDChip300 validates this technology for planetary exploration, particularly for the search for life on Mars.     

氢喷射温度对氢氧火箭发动机燃烧稳定性的影响

应用CFD方法对氢氧火箭发动机中高频燃烧不稳定性进行了数值模拟,研究分析了不同工况条件下氢喷射温度对燃烧振荡的影响规律,得出了压力振荡频率变化规律及稳定性极限图。结果表明：在一定的氢喷射温度范围内会发生不稳定燃烧,且随着混合比的增大,发生不稳定燃烧的氢喷射温度上限增大;不稳定燃烧振荡主频呈倍频关系,且在氢喷射温度（70K～110K）内,振荡主频最大。     

Taxane recovery from cells of Taxus in micro- and hypergravity

Cell suspension cultures of Taxus cuspidata produce taxanes that are released from the outer surface of cells into the culture medium as free and bound alkaloids. Paclitaxel (Taxol (TM)), is an anti-cancer drug in short supply. It has a taxane ring derived from baccatin III and a C-13 phenylisoserine side-chain. This drug is produced over a wide range of gravitational forces. Monoclonal and polyclonal antibodies to paclitaxel, baccatin III, and the C-13 phenylisoserine side chain were combined in multiple-labeling studies to localize taxanes and paclitaxel on cell surfaces or on particles released into the culture medium. Bioreactor vessel design altered the composition of taxanes recovered from cells in simulated microgravity. At 10(-2) and 2x10(-4)g, taxane recovery was reduced but biomass growth and percent paclitaxel was significantly increased. At 1 to 24g, growth was reduced with a significant recovery of total taxanes with low percent paclitaxel. Bound paclitaxel was also localized in endonuclease-rich fragmenting nuclei of individual apoptotic cells. A model is presented comprising TCH (touch) genes encoding enzymes that modify taxane-bearing xylan residues in cell walls, the calcium-sensing of gravitational forces by the cytoplasm, and the predisposition of nuclei to apoptosis. This integrates the adaptive physiological and biochemical responses of drug-producing genomes with gravitational forces.     

卡托辛对AP热分解影响的XPS研究

采用XPS方法研究了卡托辛对复合固体推进剂氧化剂AP热分解过程的作用机理。结果表明,热氧化后Fe原子的结合能向高位移动,再根据Fe2p3/2谱强度的变化,说明卡托辛在AP热分解过程中是逐渐氧化过程,Fe—C共轭键逐渐断裂;Cl原子的结合能向低位移动,且在200 eV左右出现低结合能的峰,同时其强度逐渐增大,Cl原子在200 eV处形成的低结合能态的可能途径是卡托辛的分解产物环戊二烯离子与HClO4的分解产物HCl发生反应,加速了HClO4的分解速度;卡托辛的二茂铁基夹心结构破坏后生成的Fe2O3具有较高的反应活性,亦加速AP离解产物HClO4的分解速度。     

Vibrografic signs of autonomous muscle tone studied in long term space missions

Signs of resting muscle tone, assessed by cardiac driven microvibrations (MV), were studied in two cosmonauts during long term space missions on the Russian MIR station. In the 1 g environment, MV showed the typical 7-13 Hz resonance oscillations triggered by the heart beat. In 0 g, these pulsations were damped and the waveform became similar to an acceleration ballistocardiogram. If resting tone was slightly increased by extending the arm in 0 g, the resonance oscillations reappeared in most cases. By means of a simple vibromechanic analog it is demonstrated that the elastic component of muscle has to be reduced during the fully relaxed state in 0g.     

基于精英群引导下量子粒子群算法的快速零陷形成技术

引入罚函数项,建立了一种新的发射波束方向图综合控零的多目标优化模型。用适应度值排序建立由种群非支配解组成的精英群,以精英群中的粒子作为全局最优解引导粒子飞行;用爬坡策略更新全局最优解,给出了基于精英群引导的量子粒子群算法。对阵列权值采用加权优化,可在发射波束指定方向的多零陷、宽凹口。仿真结果表明：算法对多目标的优化较文献算法更优,能优化阵列权值在指定方向快速形成零陷。     

Devising an unconventional formal logic for bioinspired spacefaring automata

The field of robotics is increasingly moving from robots confined to factory floors and assembly lines and bound to perform the same tasks over and over in an uncertainty-free, well foreseeable environment, to robots designed for operating in highly dynamic and uncertainty domains, like those of interest in space exploration. According to an idea of a “new system of formal logic less rigid than past and present formal logic” advocated by von Neumann for building a powerful theory of automata, such system should be “closer to another discipline which has been little linked in the past with logic, i.e. thermodynamics, primarily in the form it was received by Boltzmann”. Following that idea, which is particularly interesting now with the emerging computational nano-sciences, it is stressed here that a full set of isomorphisms can be established between the fundamental logical principles and the information flows, Hamiltonian or dissipative, in phase space. This form of logic, dubbed here kinetic logic, takes standard formal logic out of the field of combinatorics and into the field of the Boltzmannian form of thermodynamics, i.e. kinetics.     

连续波雷达目标回波信号参数估计的克拉美-罗界

现代雷达、导航和通信系统几乎无一例外地采用了先进的信号处理技术 [1～ 3 ] 。目前 ,最大似然估计 (ML E)方法是获得雷达目标回波信号参数估计的常用方法。它给出信号参数的无偏渐近有效估计。克拉美 -罗 (CR)界是由样本数据的联合概率密度函数导出的无偏估计量的性能界 ,它可以作为所有参数估计方法的比较标准。任何雷达关于信号参数的测量精度都不可能优于 CR界。文中给出了雷达目标回波信号参数的 CR界的详细而严格的推导过程 ,得出的 CR界公式非常简洁 ,易于计算。实验结果证明了该理论公式正确     

An examination of the carbon isotope effects associated with amino acid biosynthesis

Stable carbon isotope ratios (delta(13)C) were determined for alanine, proline, phenylalanine, valine, leucine, isoleucine, aspartate (aspartic acid and asparagine), glutamate (glutamic acid and glutamine), lysine, serine, glycine, and threonine from metabolically diverse microorganisms. The microorganisms examined included fermenting bacteria, organotrophic, chemolithotrophic, phototrophic, methylotrophic, methanogenic, acetogenic, acetotrophic, and naturally occurring cryptoendolithic communities from the Dry Valleys of Antarctica. Here we demonstrated that reactions involved in amino acid biosynthesis can be used to distinguish amino acids formed by life from those formed by nonbiological processes. The unique patterns of delta(13)C imprinted by life on amino acids produced a biological bias. We also showed that, by applying discriminant function analysis to the delta(13)C value of a pool of amino acids formed by biological activity, it was possible to identify key aspects of intermediary carbon metabolism in the microbial world. In fact, microorganisms examined in this study could be placed within one of three metabolic groups: (1) heterotrophs that grow by oxidizing compounds containing three or more carbon-to-carbon bonds (fermenters and organotrophs), (2) autotrophs that grow by taking up carbon dioxide (chemolitotrophs and phototrophs), and (3) acetoclastic microbes that grow by assimilation of formaldehyde or acetate (methylotrophs, methanogens, acetogens, and acetotrophs). Furthermore, we demonstrated that cryptoendolithic communities from Antarctica grouped most closely with the autotrophs, which indicates that the dominant metabolic pathways in these communities are likely those utilized for CO(2 )fixation. We propose that this technique can be used to determine the dominant metabolic types in a community and reveal the overall flow of carbon in a complex ecosystem.     

Effect of liquid bridge form on oscillatory thermocapillary convection under 1 g and μg conditions

This study analyses the effect of temperature difference between hot and cool disk (ΔT), and non-dimensional liquid bridge volume (V/V_o) on the transition process from steady thermocapillary convection to periodic or chaotic thermocapillary convection in a liquid bridge modeled after the floating zone method under normal gravity and microgravity conditions. From normal gravity and drop shaft experiments, the difference of the regime of the steady state and the oscillatory state was clarified on the ΔT−V/V_o plane under 1 g and μg conditions. A gap or stability region was observed in the specific V/V_o range under 1 g conditions. In the gap or stable region, after the gravity changed from 1 g to μg conditions, the temperature signals showed oscillation. From these results, the critical temperature difference under the μg conditions appeared to be smaller than that under the 1 g conditions. Temperature signals were defined as 6 different types of states. The various temperature oscillatory state regimes were obtained on a ΔT−V/V_o plane under 1 g and μg conditions. Under μg conditions, in these experimental conditions, all temperature oscillatory states exhibited only the Periodic state.     

Amino acids from ion-irradiated nitrile-containing ices

Solid CH(3)CN and solid H(2)O + CH(3)CN were ion irradiated near 10 K to initiate chemical reactions thought to occur in extraterrestrial ices. The infrared spectra of these samples after irradiation revealed the synthesis of new molecules. After the irradiated ices were warmed to remove volatiles, the resulting residual material was extracted and analyzed. Both unhydrolyzed and acid-hydrolyzed residues were examined by both liquid and gas chromatographic-mass spectral methods and found to contain a rich mixture of products. The unhydrolyzed samples showed HCN, NH(3), acetaldehyde (formed by reaction with background and atmospheric H(2)O), alkyamines, and numerous other compounds, but no amino acids. However, reaction products in hydrolyzed residues contained a suite of amino acids that included some found in carbonaceous chondrite meteorites. Equal amounts of D- and L-enantiomers were found for each chiral amino acid detected. Extensive use was made of (13)C-labeled CH(3)CN to confirm amino acid identifications and discriminate against possible terrestrial contaminants. The results reported here show that ices exposed to cosmic rays can yield products that, after hydrolysis, form a set of primary amino acids equal in richness to those made by other methods, such as photochemistry.     

一类不确定非线性系统的反演自适应控制

针对一类包括非线性不确定性、未知间隙滞后特性等的不确定非线性单输入单输出系统,结合反演和自适应控制设计了反演自适应控制器,可使系统达稳定,并能保证系统信号的全局有界性,对非线性不确定性具一定的鲁棒性,且不要求干扰项有界.由仿真结果可知:设计的反演自适应控制器的控制效果较佳.对自适应控制系统有较大的参考意义.     

变极性等离子弧焊设备及其铝合金焊接工艺研究

介绍了国产变极性等离子弧焊（VPPAW）设备的组成、变极性电源主电路的工作原理。以及微机控制和焊炬等关键技术。用该设备对不同厚度的高强度可热处理强化铝合金试样、贮箱缩比及1：1试验件进行了VPPAW，并分析了接头形式、起弧与收弧、焊接工艺参数确定、常见缺陷、焊接组织及其力学性能。焊接试验结果表明，用VPPAW设备焊接的铝舍金厚度可达14mm，与交流钨极惰性气体保护焊（TIG）相比，其焊缝质量佳，接头性能优。     

Building components for an outpost on the Lunar soil by means of a novel 3D printing technology

3D-printing technologies are receiving an always increasing attention in architecture, due to their potential use for direct construction of buildings and other complex structures, also of considerable dimensions, with virtually any shape. Some of these technologies rely on an agglomeration process of inert materials, e.g. sand, through a special binding liquid and this capability is of interest for the space community for its potential application to space exploration. In fact, it opens the possibility for exploiting in-situ resources for the construction of buildings in harsh spatial environments. The paper presents the results of a study aimed at assessing the concept of 3D printing technology for building habitats on the Moon using lunar soil, also called regolith. A particular patented 3D-printing technology – D-shape – has been applied, which is, among the existing rapid prototyping systems, the closest to achieving full scale construction of buildings and the physical and chemical characteristics of lunar regolith and terrestrial regolith simulants have been assessed with respect to the working principles of such technology. A novel lunar regolith simulant has also been developed, which almost exactly reproduces the characteristics of the JSC-1A simulant produced in the US. Moreover, tests in air and in vacuum have been performed to demonstrate the occurrence of the reticulation reaction with the regolith simulant. The vacuum tests also showed that evaporation or freezing of the binding liquid can be prevented through a proper injection method. The general requirements of a Moon outpost have been specified, and a preliminary design of the habitat has been developed. Based on such design, a section of the outpost wall has been selected and manufactured at full scale using the D-shape printer and regolith simulant. Test pieces have also been manufactured and their mechanical properties have been assessed.     

高超声速滑翔飞行器引入段弹道优化

高超声速飞行器弹道优化设计的目的足得到高超声速飞行的控制指令，从而得到方案弹道，针对其数学模型，寻找一个作为输入的攻角规律使得弹道最优。我们将此弹道问题转化成最优控制问题，通过极大值原理得到最优弹道的一阶必要条件。我们采用遗传算法求解了此两点边值问题。首先从次优化弹道得到攻角的变化规律，然后由从次优化弹道估计出的攻角范围推导出初始伴随变量的变化范围，再用遗传算法在此范崮内优化初始伴随变量得到了伞局最优弹道和相应的初始伴随变量。通过一个实例求得了满足热流约束的最大终端速度弹道，通过比较可知优于次优化弹道，从数值计算例子还可以看到遗传算法是求解两点边值问题的一种比较好的方法。     

Newton–Euler equations of multibody systems with changing structures for space applications

Multibody systems with changing structure are considered. These systems have stages in their motion that distinct from each other by degree of freedom (DOF), joint connection structure and joint types. These mechanical systems are common in space application e.g. separation subsystems. Single coordinate set is used to formulate Newton–Euler equations of motion at each stage. Proposed equation form simplifies equations building process for certain stages and whole motion. Numerical experiment was carried out using proposed method.     

扩频测控系统干扰容限的分析

扩频测控系统以其诸多优点正成为卫星测控的主流体制,经典分析给出扩频系统的干扰容限只是一个宽泛的界。在同时考虑输入信号强度、多址干扰等因素的情况下,对扩频测控系统的干扰容限上界进行分析推导,得出更符合实际情况的干扰容限上界的闭式表达,通过计算给出干扰容限与多址干扰、干扰容限与输入信噪比强度的关系。     

Reachable domain of spacecraft with a single tangent impulse considering trajectory safety

The reachable domain of the two-body transfer orbit with a single upper-bounded tangent impulse is studied. Three cases are analyzed for either the magnitude of the tangent impulse or the initial impulse point being free, or both being free. For a fixed impulse magnitude and a free initial impulse point, the initial orbit is proved to be one of the envelopes of the reachable domain. Moreover, the trajectory safety for the transfer orbit requires a lower bound on the perigee altitude and an upper bound on the apogee altitude. Then the ranges of the impulse magnitude and the initial true anomaly can be obtained by solving quadratic and cubic inequalities, respectively. If both constraints are required for an arbitrary impulse point, the range of the impulse magnitude is obtained with impulses at the perigee and the apogee. Several numerical examples with different eccentricities are provided to show the geometry of the reachable domain and to verify the proposed method.