Evaluation of gravity-dependent membrane potential shift in Paramecium.

It is still debated whether or not gravity can stimulate unicellular organisms. This question may be settled by revealing changes in the membrane potential in a manner depending on the gravitational forces imposed on the cell. We estimated the gravity-dependent membrane potential shift to be about 1 mV G⁻¹ for Paramecium showing gravikinesis at 1–5 G, on the basis of measurements of gravity-induced changes in active propulsion and those of propulsive velocity in solutions, in which the membrane potential has been measured electrophysiologically. The shift in membrane potential to this extent may occur from mechanoreceptive changes in K⁺ or Ca²⁺ conductance by about 1% and might be at the limit of electrophysiological measurement using membrane potential-sensitive dyes. Our measurements of propulsive velocity vs membrane potential also suggested that the reported propulsive force of Paramecium measured in a solution of graded densities with the aid of a video centrifuge microscope at 350 G was 11 times as large as that for −29 mV, i.e., the resting membrane potential at [K⁺]_o = 1 mM and [Ca²⁺]_o = 1 mM, and, by extrapolation, that Paramecium was hyperpolarized to −60 mV by gravity stimulation of 100- G equivalent, the value corrected by considering the reduction of density difference between the interior and exterior of the cell in the graded density solution. The estimated shift of the membrane potential from −29 mV to −60 mV by 100- G equivalent stimulation, i.e., 0.3 mV G⁻¹, could reach the magnitude entirely feasible to be measured more directly.     

Longevity in space; Experiment on the life span of Paramecium cell clone in space

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.     

Enzyme activities and membrane lipids in Artemia cysts after a long duration space flight

In the Free Flyer Biostack Experiment (L.D.E.F. mission) investigations have shown that biological objects in a resting state can survive more than 5.5 years of exposure to the space factors in particular microgravity and cosmic rays. We have measured enzyme activities involved in metabolic pathways of sugar and lipid degradation and determined phospholipid composition. Pyruvate kinase and glucose-6-phosphate dehydrogenase activities in space-exposed cysts were higher than in earth controls after 1 hour incubation. In controls, total phospholipids remained unchanged, on the contrary they increased significantly in space-exposed cysts. The rate of metabolism of various phospholipid components was unchanged in controls allowing the development while the level of most of them decreased in space-exposed cysts except for phosphatidylcholine. Enzyme activities (acetylhydrolase, phospholipase A₂ and lyso phospholipase) involved in phospholipid degradation increased; however, activities were much higher in space-exposed cysts. In conclusion, the long duration space flight resulted in an increase of the metabolic activity correlated with a faster development within the first 20 hours of post flight incubation.     

Rosat observations of single pulsars

Recent Rosat results on the X-ray emission of single neutron stars are reviewed and compared to the the existing information.     

ROSAT observations of the Chamaeleon star forming cloud

Two soft X-ray images of the Chamaeleon I star forming cloud obtained with the ROSAT Position Sensitive Proportional Counter are presented. Seventy reliable, and perhaps 19 additional, X-ray sources are found. Up to Ninety percent of these sources are certainly or probably identified with T Tauri stars formed in the cloud. Twenty to 35 are probably previously unrecognized ‘weak’ T Tauri (WTT) stars. T Tauri X-ray luminosities range from log , or 10² – 10⁴ times solar levels, with mean in the 0.2–2.5 keV band. The X-ray luminosities of well-studied Chamaeleon cloud members are correlated with a complex of four stellar properties: effective temperature, mass, radius and bolometric luminosity. The spatial distribution, H-R diagram locations of the stars indicate WTT and CTT are coeval. The total premain sequence population of the cloud is likely to be > 100 stars, with WTT stars outnumbering ‘classical’ T Tauri (CTT) stars by 2:1.     

Implications of ROSAT observations for the local hot bubble

The ROSAT all-sky survey and Guest Observer pointed observations have brought a wealth of high-quality data to the study of the soft X-ray diffuse background. Analysis of the spatial structure of the 1/4 keV flux, including observations of shadows cast by discrete clouds in the interstellar medium, have allowed the separation of the observed flux into foreground and background components supporting a spatially varying local component consistent with previous ideas of the local hot bubble, a highly variable galactic halo component, and an extragalactic background.     

Evidence that far-infrared radiation promotes growth of Xenopus laevis

In most ectotherms, environmental temperature has differential effects on growth and differentiation. For example, amphibian size at maturity decreases with increasing temperature. To address how radiant heat in the form of far-infrared radiation (FIR) may affect development of the aquatic ectotherm Xenopus laevis, we continuously irradiated swimming larvae as they developed into young adults. Here we report evidence that FIR promotes growth of these organisms in an aqueous environment.     

Growth of Prunus tree stems under simulated microgravity conditions

Stem growth of Prunus trees under simulated microgravity conditions was examined using a three-dimensional clinostat. The stems elongated with bending under such conditions. Stem elongation and leaf expansion were both promoted, whereas the formation of xylem in the secondary thickening growth was inhibited under the simulated microgravity condition. In secondary xylem, sedimentable amyloplasts were observed in the 1g control. The present results suggest that stem elongation and leaf expansion may be inhibited at 1g, while growth direction and secondary xylem formation depend on a gravity stimulus. A space experiment is expected to advance research on thickening growth in trees.     

Confirmation of gravisensitivity in the slime mold physarum polycephalum under near weightlessness

We have investigated Physarum polycephalum, a unicellular organism with no special gravity receptors, on its ability to react to gravity. The first experiments were 0 g-simulation experiments on the fast-rotating clinostat conducted with plasmodial strands of this acellular slime mold. In these earth-bound experiments the observed parameters were periodicity of the contractions and dilatations of the strand's ectoplasm as well as the periodicity and velocity of the striking cytoplasmic (endoplasmic) shuttle streaming. During 0 g-simulation these parameters showed significant changes indicating the existence of a gravisensitivity of the slime mold.

The Space-Shuttle experiment (ESA-Biorack in D 1-Mission) should demonstrate the validity of the 0 g-simulation on the fast-rotating clinostat. The experiment was designed in a way enabling the registration of the same parameters as on the clinostat (using the light microscope in combination with a photo diode and a cinecamera). Only one of the two planned measurement sessions was fully successful and provided us with data confirming the results gained on the fast-rotating clinostat: The slime mold showed under real near weightlessness in the D 1-Space Shuttle Mission a transient frequency increase in its contraction rhythmicity and a (steady) increase in the streaming velocity of its endoplasm.     

X-ray measurements of the dark matter distribution in clusters of galaxies with Chandra

We investigate the dark matter distributions in the central region of two clusters of galaxies (A1835 and MKW3S) using Chandra data. N-body simulations in the standard cold dark matter (CDM) model predict the dark matter distribution shows a cuspy dark matter profile: ρ(r) ∝ r, with in the range 1–2, while observations of dwarf and low surface brightness galaxies seem to favor the presence of a relatively flat core: 0 <  < 1. To investigate the dark matter distributions in the central region of clusters of galaxies, we analyze the Chandra data of A1835 and MKW3S with a deprojection method. We derive the mass profiles without the assumption of analytical models. We examine the inner slope of derived mass profiles assuming the dark matter profile is described with a power-law expression. The values of the slope are 0.95 ± 0.10 for A1835 and 1.33 ± 0.12 for MKW3S within the radius of 200 kpc. These are consistent with the result of the CDM simulations. However, within the radius of 100 kpc, the value of is less than unity for A1835 (0.47 ± 0.31). Our result implies that the central dark matter profile of some clusters cannot be described by CDM halos.     

Gravitropism and phototropism in protonemata of the moss Pohlia nutans (hedw.) Lindb

The gravitropism of protonemata of Pohlia nutans is described and compared with that of other mosses. In darkness, protonemata showed negative gravitropism. Under uniform illumination they grew radially over the substrate surface, whereas unilateral illumination induced positive phototropic growth. Gravitropism was coupled with starch synthesis and amyloplast formation. Protonematal gravitropic growth is more variable than the strict negative gravitropism of Ceratodon chloronema.     

Changes in the micro-structure of the vestibular apparatus of tadpoles (Rana temporaria) developed in simulated weightlessness

The vestibular apparatus of tadpoles (Rana temporaria) exposed to simulated weightlessness was examined by electron microscopy. Extended exposure to simulated weightlessness is followed by significant alterations in the sensory epithelia and also in the otolith membrane. Large vacuoles, filled with necrobiotic mitochondria and fragments of endoplasmic reticulum, were concentrated in the region where an otolith membrane covers the hair cells but were mostly absent in zones of the epithelia with undifferentiated cells. The number of otoconia in the otolith membrane was diminished. The results were compared with data from space flight experiments and some concordance was noted. The possible connection between some unusual behavior of the tadpoles after weightlessness simulation and the structural alterations in the gravitational sensors was discussed.     

Optical and electron-microscopic studies of the Funaria hygrometrica protonema after cultivation for 96 days in space

$\text{Funaria hygrometrica}$ protonema cells grown in the “IFS-2” (Inoculating fixing system) for 96 days on board the Salyut 6 — Soyuz 32 orbital scientific station were examined by light and electron-microscopy. Investigation of experimental and control cells of the moss protonema showed common features as well as distinctions in their structure. Protonema cells of $\text{Funaria hygrometrica}$ both differentiate and undergo photosynthesis during space flight. Changes in cell shape, decreased cell size, a reduction in the volume of starch granules, and altered chloroplast structure were observed.     

Evaluation of Voigt algorithms for the ISS/JEM/SMILES L2 data processing system

We present a calculation of the accuracy and speed of the Voigt function of various algorithms (Armstrong (1967), Hui et al. (1978), Humlicek (1982) and improved version of Kuntz (1997) developed in this study) for making the faster and higher accuracy forward model required for the Superconducting Submillimeter-Wave Limb-Emission Sounder (SMILES) Level 2 (L2) data processing system.     

Evaluation of SARAL/AltiKa performance using GNSS/IMU equipped buoy in Sajafi,Imam Hassan and Kangan Ports

Performance of SARAL/AltiKa mission has been evaluated within 2016 altimeter calibration/validation framework in Persian Gulf through three campaigns conducted in the offshore waters of Sajafi, Imam Hassan and Kangan Ports, while the altimeter overflew the passes 470, 111 and 25 on 13 Feb, 7 March and 17 June 2016, respectively. As the preparation, a lightweight buoy was equipped with a GNSS receiver/choke-ring antenna and a MEMS-based IMU to measure independent datasets in the field operations. To obtain accurate sea surface height (SSH) time series, the offset of the onboard antenna from the equilibrium sea level was predetermined through surveying operations as the buoy was deploying in the onshore waters of Kangan Port. Accordingly, the double-difference carrier phase observations have been processed via the Bernese GPS Software v. 5.0 so as to provide the GNSS-derived time series at the comparison points of the calibration campaigns, once the disturbing effects due to the platform tilt and heave have been eliminated. Owing to comparing of the SSH time series and the associating altimetry 1?Hz GDR-T datasets, the calibration/validation of the SARAL/AltiKa has been performed in the both cases of radiometer and ECMWF wet troposphere corrections so as to identify potential land contamination. An agreement of the present findings in comparison with those attained in other international calibrations sites confirms the promising feasibility of Persian Gulf as a new dedicated site for calibration/validation of ongoing and future altimetry missions.     

MultiWaveLink: An interactive data base for the coordination of multiwavelength and multifacility observations

MultiWaveLink is an interactive, computerized data base that was developed to facilitate a multiwavelength approach to studying astrophysical sources. It can be used to access information about multiwavelength resources (observers, telescopes, data bases and analysis facilities) or to organize observing campaigns that require either many telescopes operating in different spectral regimes or a network of similar telescopes circumspanning the Earth.     

Evaluation and analysis on positioning performance of BDS/QZSS satellite navigation systems in Asian-Pacific region

By using the observation data and products of precise obit and clock offset from Multi-GNSS Experiment (MGEX) of the International GNSS Service (IGS) and GNSS Research Centre, Curtin University in this paper, the positioning performance of BDS/QZSS satellite navigation system has been analyzed and evaluated in aspects of the quantity of visible satellites, DOP value, multipath effect, signal-to-noise ratio, static PPP and kinematic PPP. The analysis results show that compared to BDS single system when the cutoff angle are 30°and 40°, the DOP value of BDS/QZSS combined system has decreased above 20%, and the quantity of visible satellites increased about 16–30% respectively, because of the improved spatial geometric configuration. The magnitude of satellite multipath effect of BDS system shows the trend of MEO?>?IGSO?>?GEO, which is consistent with that of QZSS satellite system, as the constellation structure of the two systems is similar. The variation tendencies of signal-to-noise ratio with respect to elevation angle of the two systems are almost the same at all frequencies, showing that at the same elevation angle the signal-to-noise ratio of MEO satellites is higher than that of IGSO satellites, as the higher obit is the lower transmitting power is obtained. For having a specially designed obit, the variation of signal-to-noise ratio of BDS system is more stable. However, the magnitude of signal-to-noise ratio of QZSS system appears the trend of frequency 3?>?frequency 2?>?frequency 1. The static PPP performance of the BDS/QZSS combination system has been improved more significantly than the BDS single system in E, N and U directions. When the cutoff angle are at 7°, 15° and 30°, the PPP accuracy is increased about 25–34% in U direction, 10–13% and 23–34% in E and N directions respectively. When the elevation angle is large (40°), compared to BDS single system at lower elevation angles (7° and 15°) the PPP accuracy of the BDS/QZSS combination system is improved above 30% in U direction. In kinematic PPP performance, compared to BDS single system, the accuracy, availability and reliability of the BDS/QZSS combination system has been improved too, especially at large elevation angles (30° and 40°), the kinematic PPP accuracy in E and U directions has been improved about 10–50%, and above 50% in U direction. It can be concluded that the combination with QZSS system can improve the positioning accuracy, reliability and stability of BDS system. In the future, with the improvement of the satellite construction of Japan’s QZSS system and the global networking of China’s BDS satellites, the QZSS satellites will contribute greatly to improve the positioning accuracy, reliability, availability and stability of GNSS systems in areas such as cities, mountains, densely-packed buildings and severely covered areas in Asian-Pacific region.     

Space flight effects on paramecium tetraurelia flown aboard Salyut 6 in the Cytos I and Cytos M experiments

Results of the Cytos M experiment and complementary results of the Cytos I experiment flown aboard the Soviet orbital station Salyut 6 are shown. Space flight of Paramecia cultures resulted in a stimulating effect on cell proliferation, in a larger cell volume, in changes in cell dry weight, cell total protein and the electrolyte content of the culture media in which the organisms were grown. The assumption of a possible effect of weightlessness on membrane permeability is discussed.     

Changes in membrane lipid composition in azuki bean epicotyls under hypergravity conditions: Possible role of membrane sterols in gravity resistance

Seedlings of azuki bean (Vigna angularis Ohwi et Ohashi) were cultivated under hypergravity conditions, and changes in membrane lipid composition in their epicotyls were analyzed. Under hypergravity conditions at 300g, the levels of total sterols, phospholipids, and fatty acids per fresh weight were kept higher, as compared with 1g controls. In particular, sterol levels were prominently increased by hypergravity. On the other hand, hypergravity did not clearly influence the levels of each phospholipid and glycolipid class, or their fatty acid compositions. Thus, the effect of hypergravity on membrane lipid metabolism was specific for sterol biosynthesis. In various regions of azuki epicotyls, high growth rate was associated with high sterol levels. Hypergravity suppressed elongation growth and stimulated lateral expansion of azuki epicotyls. In the presence of lovastatin, an inhibitor of sterol biosynthesis, at 30 μM, such changes in growth parameters occurred even under 1g conditions, suggesting that lovastatin made epicotyls hypersensitive to the gravitational force. These results support the hypothesis that membrane sterols are involved in maintenance of normal growth capacity of plant organs against gravity.