Planetary protection policy overview and application to future missions.

The 1967 treaty on the peaceful uses of outer space reflected both concerns associated with the unknown nature of the space environment and the desire of the world scientific community to preserve the pristine nature of celestial objects until such times as they could be studied in an effective manner. Since 1967, NASA has issued policy directives that have adopted the guidelines of COSPAR for protecting the planets from contamination by Earth organisms and for protecting the Earth from the unknown. This paper presents the current status of planetary protection (quarantine) policy within NASA, and a prospectus on how planetary protection and back contamination issues might be addressed in relation to future missions envisioned for development by NASA either independently, or in cooperation with the space agencies of other nations.     

The effect of artificial gravity on plasma and tissue lipids in rats: The Cosmos 936 experiment

Plasma and tissue lipids in male SPF Wistar rats flown for 18.5 days aboard the Cosmos 936 biosatellite were analyzed. One group of rats was subjected to artificial gravity by use of a centrifuge during the flight. An experiment simulating known space flight factors other than weightlessness was done on Earth. An increase of total cholesterol in plasma, of nonesterified fatty acids in plasma and brown adipose tissue, of triacylglycerols in plasma, liver, thymus and bone marrow was noted several hours after biosatellite landing. Smaller changes were observed in the terrestrial control experiment. With the exception of triacylglycerol accumulation in bone marrow, these increases disappeared 25 days after biosatellite landing. Exposing the rats aboard the biosatellite to artificial gravity was beneficial in the sense that such exposure inhibited the phospholipid and triacylglycerol increase in plasma and inhibited the increase of triacylglycerol in liver and especially in bone marrow.     

Effects of microgravity on bone and calcium homeostasis.

Mechanical function is known to be of crucial importance for the maintenance of bone tissue. Gravity on one hand and muscular effort on the other hand are required for normal skeletal structure. It has been shown by numerous experimental studies that loss of total-body calcium, and marked skeletal changes occur in people who have flown in space. However, most of the pertinent investigations have been conducted on animal models, including rats and non-human primates, and a reasonably clear picture of bone response to spaceflight has emerged during the past few years. Osteopenia induced by microgravity was found to be associated with reduction in both cortical and trabecular bone formation, alteration in mineralization patterns and disorganization of collagen, and non-collagenous protein metabolism. Recently, cell-culture techniques have offered a direct approach of altered gravity effects at the osteoblastic-cell level. But the fundamental mechanisms by which bone and calcium are lost during spaceflight are not yet fully known. Infrequency and high financial cost of flights have created the necessity to develop on-Earth models designed to mimic weightlessness effects. Antiorthostatic suspension devices are now commonly used to obtain hindlimb unloading in rats, with skeletal effects similar to those observed after spaceflight. Therefore, actual and "simulated" spaceflights, with investigations conducted at whole body and cellular levels, are needed to elucidate pathogeny of bone loss in space, to develop effective countermeasures, and to study recovery processes of bone changes after return to Earth.     

Remote sensing of aerosol and radiation from geostationary satellites

The paper presents a high-level overview of current and future remote sensing of aerosol and shortwave radiation budget carried out at the US National Oceanic and Atmospheric Administration (NOAA) from the US Geostationary Operational Environmental Satellite (GOES) series. The retrievals from the current GOES imagers are based on physical principles. Aerosol and radiation are estimated in separate processing from the comparison of satellite-observed reflectances derived from a single visible channel with those calculated from detailed radiative transfer. The radiative transfer calculation accounts for multiple scattering by molecules, aerosol and cloud and absorption by the major atmospheric gases. The retrievals are performed operationally every 30 min for aerosol and every hour for radiation for pixel sizes of 4-km (aerosol) and 15- to 50-km (radiation). Both retrievals estimate the surface reflectance as a byproduct from the time composite of clear visible reflectances assuming fixed values of the aerosol optical depth. With the launch of GOES-R NOAA will begin a new era of geostationary remote sensing. The Advanced Baseline Imager (ABI) onboard GOES-R will offer capabilities for aerosol remote sensing similar to those currently provided by the Moderate Resolution Imaging Spectroradiometer (MODIS) flown on the NASA Earth Observing System (EOS) satellites. The ABI aerosol algorithm currently under development uses a multi-channel approach to estimate the aerosol optical depth and aerosol model simultaneously, both over water and land. Its design is strongly inspired by the MODIS aerosol algorithm. The ABI shortwave radiation budget algorithm is based on the successful GOES Surface and Insolation Product system of NOAA and the NASA Clouds and the Earth’s Radiant Energy System (CERES), Surface and Atmospheric Radiation Budget (SARB) algorithm. In all phases of the development, the algorithms are tested with proxy data generated from existing satellite observations and forward simulations. Final assessment of the performance will be made after the launch of GOES-R scheduled in 2012.     

Preparing for space: Increasing technical readiness of low-cost high-performance remote sensing using high-altitude ballooning

Two widely available, small size, weight and power camera systems were flown above 97 % of Earth’s atmosphere and showed utility in single filter vegetation and soil analysis in a space analogue environment. The experiment was conducted as a low-cost verification and test analogue to flying on vastly more expensive low Earth orbit missions. Normalised Difference Vegetation Index (NDVI) was used as the metric by which performance was analysed for ground calibration testing, low and near-space altitude remote sensing. Ground calibration testing with a laboratory-grade spectrometer revealed that both cameras were able to return consistent NDVI results, and high-altitude balloon flight allowed similar data capture from an environment similar to space. Although compressed captured imagery had been processed using gamma correction and pre-image processing, these were able to be corrected provided that access to radiometrically-calibrated data was available. The two hobbyist cameras were shown to return scientifically useful results, demonstrating performance, and additionally their utility for citizen science applications in the near-space environment.     

Parathyroid hormone-related protein is a gravisensor in lung and bone cell biology.

Parathyroid Hormone-related Protein (PTHrP) has been shown to be essential for the development and homeostatic regulation of lung and bone. Since both lung and bone structure and function are affected by microgravity, we hypothesized that 0 x g down-regulates PTHrP signaling. To test this hypothesis, we suspended lung and bone cells in the simulated microgravity environment of a Rotating Wall Vessel Bioreactor, which simulates microgravity, for up to 72 hours. During the first 8 hours of exposure to simulated 0 x g, PTHrP expression fell precipitously, decreasing by 80-90%; during the subsequent 64 hours, PTHrP expression remained at this newly established level of expression. PTHrP production decreased from 12 pg/ml/hour to 1 pg/ml/hour in culture medium from microgravity-exposed cells. The cells were then recultured at unit gravity for 24 hours, and PTHrP expression and production returned to normal levels. Based on these findings, we have obtained bones from rats flown in space for 2 weeks (Mission STS-58, SL-2). Analysis of PTHrP expression by femurs and tibias from these animals (n=5) revealed that PTHrP expression was 60% lower than in bones from control ground-based rats. Interestingly, there were no differences in PTHrP expression by parietal bone from space-exposed versus ground-based animals, indicating that the effect of weightlessness on PTHrP expression is due to the unweighting of weight-bearing bones. This finding is consistent with other studies of microgravity-induced osteoporosis. The loss of the PTHrP signaling mechanism may be corrected using chemical agents that up-regulate this pathway. In conclusion, PTHrP represents a stretch-sensitive paracrine signaling mechanism that may sense gravity.     

Animal research facility for Space Station Freedom.

An integrated animal research facility is planned by NASA for Space Station Freedom which will permit long-term, man-tended experiments on the effects of space conditions on vertebrates. The key element in this facility is a standard type animal habitat which supports and maintains the animals under full bioisolation during transport and during the experiment. A holding unit accommodates the habitats with animals to be maintained at zero gravity; and a centrifuge, those to be maintained at artificial gravity for control purposes or for gravity threshold studies. A glovebox permits handling of the animals for experimental purposes and for transfer to a clean habitat. These facilities are described, and the aspects of environmental control, monitoring, and bioisolation are discussed.     

Planetary Protection Policy (U.S.A.).

Through existing treaty obligations of the United States, NASA is committed to exploring space while avoiding biological contamination of the planets, and to the protection of the Earth against harm from materials returned from space. Because of the similarities between Mars and Earth, plans for the exploration of Mars evoke discussions of these Planetary Protection issues. US Planetary Protection Policy will be focused on the preservation of these goals in an arena that will change with the growth of scientific knowledge about the martian environment. Early opportunities to gain the appropriate data will be used to guide later policy implementation. Because human presence on Mars will result in the end of Earth's separation from the martian environment, it is expected that precursor robotic missions will address critical planetary protection concerns before humans arrive.     

Urodelean amphibians in studies on microgravity: effects upon organ and tissue regeneration.

Results obtained from nine experiments performed onboard Russian biosatellites have shown that microgravity promotes tissue regeneration in the newt, Pleurodeles waltl. The effect has been reproduced in all flights and on a clinostat as well for eye tissues (lens and retina), limbs and tail. The effect was demonstrated in 1.5- to 2-fold increase in cell proliferation in the early stages of regeneration in space flight. Animals "flown" intact and operated after flight regenerated faster than control ones and showed long-lasting micro-"g" effect. The most recent experiment flew aboard the Bion-11 biosatellite. This test was performed for study on microgravity effect on neural retina regeneration after optic nerve lesioning in the newt. Obtained results confirmed our previous information about intensification of regenerative processes in detached neural retina in urodela exposed to simulated weightlessness (Grigoryan et al., 1998). In particular, we found the increase and activation of cell populations participating in neural retina restoration and maintenance of retinal structure. Our findings suggest that promoting effect of microgravity upon regeneration could be influenced by several factors, largely influenced by a response of the whole organism to changed gravity vector. We hypothesized the synthesis of the specific range of stress proteins induced by micro-"g" and their regulative role in cell proliferation. Such a hypothesis for the existence of "altered gravity stress proteins" is discussed.     

Characteristics and accuracies of the GRACE inter-satellite pointing

For almost 10 years, the Gravity Recovery and Climate Experiment (GRACE) has provided information about the Earth gravity field with unprecedented accuracy. Efforts are ongoing to approach the GRACE baseline accuracy as there still remains an order of magnitude between the present error level of the gravity field solutions and the GRACE baseline. At the current level of accuracy, thorough investigation of sensor related effects is necessary as they are one of the potential contributors to the error budget. In the science mode operations, the twin satellites are kept precisely pointed with their KBR antennas towards each other. It is the task of the onboard attitude and orbit control system (AOCS) to keep the satellites in the required formation. We analyzed long time series of the inter-satellite pointing variations as they reflect the AOCS performance and characteristics. We present significant systematic effects in the inter-satellite pointing and discuss their possible sources. Prominent features are especially related to the magnetic torquer characteristics, star cameras’ performance and KBR antenna calibration parameters. The relation between the magnetic torquer attitude control and the Earth magnetic field, impact of the different performance of the two star camera heads on the attitude control and the features due to uncertainties in the calibration parameters relating the star camera frame to K-frame are discussed in detail. Proper understanding of these effects will help to reduce their impact on the science data and subsequently increase the accuracy of the gravity field solutions. Moreover, understanding the complexity of the onboard system is essential not only for increasing the accuracy of the GRACE data but also for the development of the future gravity field satellite missions.     

Accommodations for earth-viewing payloads on the international space station

The design of the International Space Station (ISS) includes payload locations that are external to the pressurized environment. These external or attached payload accommodation locations will allow direct access to the space environment at the ISS orbit and direct viewing of the earth and space. NASA sponsored payloads will have access to several different types of standard external locations; the S3 Truss Sites, the Columbus External Payload Facility (EPF), and the Japanese Experiment Module Exposed Facility (JEM-EF). As the ISS Program develops, it may also be possible to locate external payloads at the P3 Truss Sites or at non-standard locations similar to the handrail-attached payloads that were flown during the MIR Program. Earth-viewing payloads may also be located within the pressurized volume of the US Lab in the Window Observational Research Facility (WORF). Payload accommodations at each of the locations will be described, as well as transport to and retrieval from the site.     

Science Research and Utilization Planning of China's Space Station in Operation Period 2022-2032

The core module of China's Space Station (CSS) is scheduled to be launched around the end of 2020, and the experimental module I and II will be launched in the next two years. After on-orbit constructions, CSS will be transferred into an operation period over 10 years (2022-2032 and beyond) to continuously implement space science missions. At present, based on the project selection and research work in the ground development period of CSS, China is systematically making a utilization mission planning for the operation period, which focuses on the fields of aerospace medicine and human research, space life science and biotechnology, microgravity fluid physics, combustion science, materials science, fundamental physics, space astronomy and astrophysics, Earth science, space physics and space environment, space application technology, etc. In combination with the latest development trend of space science and technology, China will continue to update planning for science research and technology development, carry out project cultivation, payload R&D, and upgrade onboard and ground experiment supporting systems to achieve greater comprehensive benefits in science, technology, economy, and society.      

Bodies in flowing plasmas: Laboratory studies

A brief review of early laboratory investigations of bodies in flowing, rarefied plasmas is given together with a discussion of more recent parametric studies carried out at NASA/Marshall Space Flight Center (MSFC), which include the effects of the ion acoustic Mach number and the normalized test body potential. Good agreement is found between the experimental results and theoretical calculations which omit ion thermal motion. The relation between laboratory investigations and the results of satellite-borne measurements is addressed. This relationship has led to an appreciation for the benefits of applying the methods and techniques of laboratory plasma physics to investigations in space, where several limitations inherent to the laboratory can be circumvented. These types of investigations, conducted in Earth orbit, can enhance our understanding of space plasma physics and have direct application to certain types of solar system phenomena.     

CELSS transportation analysis

Regenerative life support systems based on the use of biological material have been considered for inclusion in manned spacecraft since the early days of the United States space program. These biological life support systems are currently being developed by NASA in the Controlled Ecological Life Support System (CELSS) program. Because of the progress being achieved in the CELSS program, it is time to determine which space missions may profit from use of the developing technology. This paper presents the results of a study that was conducted to estimate where potential transportation cost savings could be anticipated by using CELSS technology for selected future manned space missions.

Six representative missions were selected for study from those included in NASA planning studies. The selected missions ranged from a low Earth orbit mission to those associated with asteroids and a Mars sortie. The crew sizes considered varied from four persons to five thousand. Other study parameters included mission duration and life support closure percentages, with the latter ranging from complete resupply of consumable life support materials to 97% closure of the life support system. The paper presents the analytical study approach and describes the missions and systems considered, together with the benefits derived from CELSS when applicable.     

Recent advances in technologies required for a "Salad Machine".

Future long duration, manned space flight missions will require life support systems that minimize resupply requirements and ultimately approach self-sufficiency in space. Bioregenerative life support systems are a promising approach, but they are far from mature. Early in the development of the NASA Controlled Ecological Life Support System Program, the idea of onboard cultivation of salad-type vegetables for crew consumption was proposed as a first step away from the total reliance on resupply for food in space. Since that time, significant advances in space-based plant growth hardware have occurred, and considerable flight experience has been gained. This paper revisits the "Salad Machine" concept and describes recent developments in subsystem technologies for both plant root and shoot environments that are directly relevant to the development of such a facility.     

Review of primary spaceflight-induced and secondary reloading-induced changes in slow antigravity muscles of rats.

We have examined the light and electron microscopic properties of hindlimb muscles of rats flown in space for 1-2 weeks on Cosmos biosatellite flights 1887 and 2044 and Space Shuttle missions Spacelab-3, Spacelab Life Sciences-1 and Spacelab Life Sciences-2. Tissues were obtained both inflight and postflight permitting definition of primary microgravity-induced changes and secondary reentry and gravity reloading-induced alterations. Spaceflight causes atrophy and expression of fast fiber characteristics in slow antigravity muscles. The stresses of reentry and reloading reveal that atrophic muscles show increased susceptibility to interstitial edema and ischemic-anoxic necrosis as well as muscle fiber tearing with disruption of contractile proteins. These results demonstrate that the effects of spaceflight on skeletal muscle are multifaceted, and major changes occur both inflight and following return to Earth's gravity.     

The role of gravity in the evolutionary emergence of multicellular complexity: Microgravity effects on arthropod development and aging

While experiments carried out in Space with isolated cells have shown that eucaryotic cells are able to sense and respond to the absence of gravity by modifying their reactions, experiments in which more complex processes have been investigated, such as Biological Systems undergoing development under Microgravity, have been surprisingly unaffected by the space environment. This can be considered a curious result since all organisms are evolutionarily adapted to the current level of the gravity force in our planet and should eventually change if this parameter will vary in a permanent manner. In fact, the small effects of the modifications in gravity on development in short term experiments may be equivalent to the difficulties in detecting the involvement of other basic physical processes such as diffusion-controled auto-organizative reactions in currently developing biological systems. An apparent exception to this lack of effect is experiments where brine shrimp dormant gastrulae directly exposed to the space environment accumulate developmental defects as a consequence of cosmic irradiation. In this article we discuss the idea that at a certain stage during the evolutionary emergence of multicellular organisms the cues laid by generic forces such as gravity were involved in the evolutionary organization of these primitive organisms. As evolution proceed, these early mechanisms may have been obscured and/or made redundant by the appearance of new internal, environment-independent biological regulatory mechanisms. On the other hand, behavioral responses that may be important, for example, in setting the life-spans of organisms may still be more readily susceptible to manipulation by external cues as experiments carried out by our group in Space and on the ground with Drosophila melanogaster indicate.