Extremophiles and the search for extraterrestrial life

Extremophiles thrive in ice, boiling water, acid, the water core of nuclear reactors, salt crystals, and toxic waste and in a range of other extreme habitats that were previously thought to be inhospitable for life. Extremophiles include representatives of all three domains (Bacteria, Archaea, and Eucarya); however, the majority are microorganisms, and a high proportion of these are Archaea. Knowledge of extremophile habitats is expanding the number and types of extraterrestrial locations that may be targeted for exploration. In addition, contemporary biological studies are being fueled by the increasing availability of genome sequences and associated functional studies of extremophiles. This is leading to the identification of new biomarkers, an accurate assessment of cellular evolution, insight into the ability of microorganisms to survive in meteorites and during periods of global extinction, and knowledge of how to process and examine environmental samples to detect viable life forms. This paper evaluates extremophiles and extreme environments in the context of astrobiology and the search for extraterrestrial life.     

Pumice as a remarkable substrate for the origin of life

The context for the emergence of life on Earth sometime prior to 3.5 billion years ago is almost as big a puzzle as the definition of life itself. Hitherto, the problem has largely been addressed in terms of theoretical and experimental chemistry plus evidence from extremophile habitats like modern hydrothermal vents and meteorite impact structures. Here, we argue that extensive rafts of glassy, porous, and gas-rich pumice could have had a significant role in the origin of life and provided an important habitat for the earliest communities of microorganisms. This is because pumice has four remarkable properties. First, during eruption it develops the highest surface-area-to-volume ratio known for any rock type. Second, it is the only known rock type that floats as rafts at the air-water interface and then becomes beached in the tidal zone for long periods of time. Third, it is exposed to an unusually wide variety of conditions, including dehydration. Finally, from rafting to burial, it has a remarkable ability to adsorb metals, organics, and phosphates as well as to host organic catalysts such as zeolites and titanium oxides. These remarkable properties now deserve to be rigorously explored in the laboratory and the early rock record.     

With a grain of salt: what halite has to offer to discussions on the origin of life

This experimental study investigated how the dynamics of the crystallization of the evaporite mineral halite could affect the accumulation and preservation of organic macromolecules present in the crystallizing solution. Halite was grown under controlled conditions in the presence of polymer nanoparticles that acted as an analog to protocellular material. Optical microscopy, atomic force microscopy, and laser scanning confocal fluorescence microscopy were used to trace the localization of the nanoparticles during and after growth of halite crystals. The present study revealed that the organic nanoparticles were not regularly incorporated within the halite, but were very concentrated on its surfaces. Their distribution was controlled dominantly by the morphologic surface features of the mineral rather than by specific molecular interactions with an atomic plane of the mineral. This means that the distribution of organic molecules was controlled by surfaces like those of halite's evaporitic growth forms. The experiments with halite also demonstrated that a mineral need not continuously incorporate organic molecules during its crystallization to preserve those molecules: After rejection by (non-incorporation into) the crystallizing halite, the organic nanoparticles increased in concentration in the evaporating brine. They ultimately either adsorbed in rectilinear patterns onto the hopper-enhanced surfaces and along discontinuities within the crystals, or they were encapsulated within fluid inclusions. Of additional importance in origin-of-life considerations is the fact that halite in the natural environment rapidly can change its role from that of a protective repository (in the absence of water) to that of a source of organic particles (as soon as water is present) when the mineral dissolves.     

Experimentally tracing the key steps in the origin of life: The aromatic world

Life is generally believed to emerge on Earth, to be at least functionally similar to life as we know it today, and to be much simpler than modern life. Although minimal life is notoriously difficult to define, a molecular system can be considered alive if it turns resources into building blocks, replicates, and evolves. Primitive life may have consisted of a compartmentalized genetic system coupled with an energy-harvesting mechanism. How prebiotic building blocks self-assemble and transform themselves into a minimal living system can be broken into two questions: (1) How can prebiotic building blocks form containers, metabolic networks, and informational polymers? (2) How can these three components cooperatively organize to form a protocell that satisfies the minimal requirements for a living system? The functional integration of these components is a difficult puzzle that requires cooperation among all the aspects of protocell assembly: starting material, reaction mechanisms, thermodynamics, and the integration of the inheritance, metabolism, and container functionalities. Protocells may have been self-assembled from components different from those used in modern biochemistry. We propose that assemblies based on aromatic hydrocarbons may have been the most abundant flexible and stable organic materials on the primitive Earth and discuss their possible integration into a minimal life form. In this paper we attempt to combine current knowledge of the composition of prebiotic organic material of extraterrestrial and terrestrial origin, and put these in the context of possible prebiotic scenarios. We also describe laboratory experiments that might help clarify the transition from nonliving to living matter using aromatic material. This paper presents an interdisciplinary approach to interface state of the art knowledge in astrochemistry, prebiotic chemistry, and artificial life research.     

The possible origin and persistence of life on Enceladus and detection of biomarkers in the plume

The jets of icy particles and water vapor issuing from the south pole of Enceladus are evidence for activity driven by some geophysical energy source. The vapor has also been shown to contain simple organic compounds, and the south polar terrain is bathed in excess heat coming from below. The source of the ice and vapor, and the mechanisms that accelerate the material into space, remain obscure. However, it is possible that a liquid water environment exists beneath the south polar cap, which may be conducive to life. Several theories for the origin of life on Earth would apply to Enceladus. These are (1) origin in an organic-rich mixture, (2) origin in the redox gradient of a submarine vent, and (3) panspermia. There are three microbial ecosystems on Earth that do not rely on sunlight, oxygen, or organics produced at the surface and, thus, provide analogues for possible ecologies on Enceladus. Two of these ecosystems are found deep in volcanic rock, and the primary productivity is based on the consumption by methanogens of hydrogen produced by rock reactions with water. The third ecosystem is found deep below the surface in South Africa and is based on sulfur-reducing bacteria consuming hydrogen and sulfate, both of which are ultimately produced by radioactive decay. Methane has been detected in the plume of Enceladus and may be biological in origin. An indicator of biological origin may be the ratio of non-methane hydrocarbons to methane, which is very low (0.001) for biological sources but is higher (0.1-0.01) for nonbiological sources. Thus, Cassini's instruments may detect plausible evidence for life by analysis of hydrocarbons in the plume during close encounters.     

Archaeal genome organization and stress responses: implications for the origin and evolution of cellular life

For DNA to be used as an informational molecule it must exist in the cell on the edge of stability because all genomic processes require local controlled melting. This presents mechanistic opportunities and problems for genomic DNA from hyperthermophilic organisms, whose unpackaged DNA could melt at optimal temperatures for growth. Hyperthermophiles are suggested to employ the novel positively supercoiling topoisomerase enzyme reverse gyrase (RG) to form positively supercoiled DNA that is intrinsically resistant to thermal denaturation. RG is presently the only archaeal gene that is uniquely found in hyperthermophiles and therefore is central to hypotheses suggesting a hypothermophilic origin of life. However, the suggestion that RG has evolved by the fusion of two pre-existing enzymes has led to hypotheses for a lower temperature for the origin of life. In addition to the action of topoisomerases, DNA packaging and the intracellular ionic environment can also manipulate DNA topology significantly. In the Euryarchaeota, nucleosomes containing minimal histones can adopt two alternate DNA topologies in a salt-dependent manner. From this we hypothesize that since internal salt concentrations are increased following an increase in temperature, the genomic effects of temperature fluctuations could also be accommodated by changes in nucleosome organization. In addition, stress-induced changes in the nucleoid proteins could also play a role in maintaining the genome in the optimal topological state in changing environments. The function of these systems could therefore be central to temperature adaptation and thus be implicated in origin of life scenarios involving hyperthermophiles.     

Formation of nucleobases from formamide in the presence of iron oxides: implication in chemical evolution and origin of life

Simple compounds like HCN, which have one C and one N, are proposed as the probable precursors for biomonomers. Formamide, a hydrolysis product of HCN, is known as the precursor of various biologically important compounds, for example, nucleobases (purines and pyrimidines). In this paper, we report our results on the synthesis of nucleobases, adenine, cytosine, purine, 9-(hydroxyacetyl) purine, and 4(3H)-pyrimidinone from formamide, using iron oxide (hematite) and oxide hydroxides (goethite and akaganeite) as a catalyst. Goethite and hematite produced purine in higher yield. The products formed were characterized by high-performance liquid chromatography and electrospray ionization mass spectrometry techniques. Results of our study reveal that iron oxides might have worked as efficient prebiotic catalysts.     

Scenarios which may lead to the rise of an asteroid-based technical civilisation

In a previous paper, the author described a hypothetical development path of technical civilisations which has the following stages: planet dwellers, asteroid dwellers, interstellar travellers, interstellar space dwellers. In this paper, several scenarios are described which may cause the rise of an asteroid-based technical civilisation. Before such a transition may take place, certain space technologies must be developed fully (now these exist only in very preliminary forms): closed-cycle biological life support systems, space manufacturing systems, electrical propulsion systems. After mastering these technologies, certain events may provide the necessary financial means and social impetus for the foundation of the first asteroid-based colonies. In the first scenario, a rich minority group becomes persecuted and they decide to leave the Earth. In the second scenario, a "cold war"-like situation exists and the leaders of the superpowers order the creation of asteroid-based colonies to show off their empires' technological (and financial) grandiosity. In the third scenario, the basic situation is similar to the second one, but in this case the asteroids are not just occupied by the colonists. With several decades of hard work, an asteroid can be turned into a kinetic energy weapon which can provide the same (or greater) threat as the nuclear arsenal of a present superpower. In the fourth scenario, some military asteroids are moved to Earth-centred orbits and utilised as "solar power satellites" (SPS). This would be a quite economical solution because a "military asteroid" already contains most of the important components of an SPS (large solar collector arrays, power distribution devices, orbit modifying rocket engine), one should add only a large microwave transmitter.     

Rock surfaces as life indicators: new ways to demonstrate life and traces of former life

Life and its former traces can only be detected from space when they are abundant and exposed to the planetary atmosphere at the moment of investigation by orbiters. Exposed rock surfaces present a multifractal labyrinth of niches for microbial life. Based upon our studies of highly stress-resistant microcolonial fungi of stone monument and desert rock surfaces, we propose that microbial biofilms that develop and become preserved on rock surfaces can be identified remotely by the following characteristics: (1) the existence of spectroscopically identifiable compounds that display unique adsorption, diffraction, and reflection patterns characteristic of biogenerated organic compounds (e.g., chlorophylls, carotenes, melanins, and possibly mycosporines), (2) demonstrably biogenic geomorphological features (e.g., biopitting, biochipping, and bioexfoliation), and (3) biominerals produced in association with biofilms that occupy rock surfaces (e.g., oxalates, forsterite, and special types of carbonates, sulfides, and silicates). Such traces or biosignatures of former life could provide macroscopically visible morphotypes and chemically identifiable products uniquely indicative of life.     

Hydrothermal simulation experiments as a tool for studies of the origin of life on Earth and other terrestrial planets: a review

The potential of life's origin in submarine hydrothermal systems has been evaluated by a number of investigators by conducting high temperature-high pressure experiments involving organic compounds. In the majority of these experiments little attention has been paid to the importance of constraining important parameters, such as the pH and the redox state of the system. This is particularly revealed in the apparent difficulties in interpreting experimental data from hydrothermal organic synthesis and stability studies. However, in those cases where common mineral assemblages have been used in an attempt to buffer the pH and redox conditions to geologically and geochemically realistic values, theoretical and experimental data seem to converge. The use of mineral buffer assemblages provides a convenient way by which to constrain the experimental conditions. Studies at high temperatures and pressure in the laboratory have revealed a number of reactions that proceed rapidly in hydrothermal fluids, including the Strecker synthesis of amino acids. In other cases, the verification of postulated abiotic reaction mechanisms has not been possible, at least for large molecules such as large fatty acids and hydrocarbons. This includes the Fischer-Tropsch synthesis reaction. High temperature-high pressure experimental methods have been developed and used successfully for a long time in, for example, mineral solubility studies under hydrothermal conditions. By taking advantage of this experimental experience new and, at times, unexpected directions can be taken in bioorganic geochemistry, one being, for instance, primitive two-dimensional information coding. This article critically reviews some of the organic synthesis and stability experiments that have been conducted under simulated submarine hydrothermal conditions. We also discuss some of the theoretical and practical considerations that apply to hydrothermal laboratory studies of organic molecules related to the origin of life on Earth and probably also to the other terrestrial planets.     

Property rights and the duty to extend human life

This article examines what the author calls the ‘argument from duty’, i.e. the argument that we should recognize private property rights in space because doing so will help us to act upon our duty to ensure the future survival of humanity. More strongly, the motivational impact of recognizing such rights may be required if we are to act upon this duty in an effective manner. The article argues that the duty in question is important but not overriding and that there are other duties with which it may happen to conflict. More specifically, the practical requirements of human resettlement may conflict with our duty to pursue a more just and environmentally sensitive way of living here on Earth. If this is correct, only a significantly weakened argument from duty may be made. The article also strengthens the case for thinking about our duties in the light of other ethical considerations.     

A non-earthcentric approach to life detection

The ultimate goal of a comprehensive life detection strategy is never to miss life when we encounter it. To accomplish this goal, we must define life in universal, that is, non-Earthcentric, measurable terms. Next, we must understand the nature of biosignatures observed from the measured parameters of life. And finally, we must have a clear idea of the end-member states for the search--what does life, past life, or no life look like (in terms of the measured parameters) at multiple spatial and temporal scales? If we can approach these problems both in the laboratory and in the field on Earth, then we have a chance of being able to detect life elsewhere in our solar system. What are the required limits of detection at each of those scales? What spatial, spectral, and temporal resolutions are necessary to detect life? These questions are actively being investigated in our group, and in this report, we present our strategy and approach to non-Earthcentric life detection.     

On the origin of satellite swarms

For a species to develop in nature basically two things are needed: an enabling technology and a “niche”. In spacecraft design the story is the same. Both a suitable technology and a niche application need to be there before a new generation of spacecraft can be developed. In the last century two technologies have emerged which had and still have a huge impact on the development of technical systems: Micro-Electronics (ME) and Micro-Systems Technology (MST). Many different terrestrial systems have changed dramatically since the introduction of ME and MST and many new systems have emerged. In the same period many nano-satellites have been built and launched and shown that they can perform in space. Still it is not clear what the specific role of these small satellites will be. Where will they go? What will they do? In this paper the authors will try to answer these questions and will refer to the OLFAR space born radio telescope as one of the niche applications for a nano-satellite swarm.     

赛博空间作战及应考虑的问题

电子信息系统及其网络在信息战争中的重要性,决定了它必将成为战争中的首要攻击目标。这种攻击已不再局限于火力摧毁和电子干扰等传统手段,正逐步演变成为信息战争中一种全新的作战模式,即赛博空间作战。赛博空间作战目前还不成熟,有些技术还处于发展阶段,有些理论还处于探索阶段。简要阐述美国赛博空间作战概念及其对赛博空间作战能力的要求,指出了赛博空间作战的作用和应考虑的问题。     

Formation of replicating saponite from a gel in the presence of oxalate: implications for the formation of clay minerals in carbonaceous chondrites and the origin of life

The potential role of clay minerals in the abiotic origin of life has been the subject of ongoing debate for the past several decades. At issue are the clay minerals found in a class of meteorites known as carbonaceous chondrites. These clay minerals are the product of aqueous alteration of anhydrous mineral phases, such as olivine and orthopyroxene, that are often present in the chondrules. Moreover, there is a strong correlation in the occurrence of clay minerals and the presence of polar organic molecules. It has been shown in laboratory experiments at low temperature and ambient pressure that polar organic molecules, such as the oxalate found in meteorites, can catalyze the crystallization of clay minerals. In this study, we show that oxalate is a robust catalyst in the crystallization of saponite, an Al- and Mg-rich, trioctahedral 2:1 layer silicate, from a silicate gel at 60°C and ambient pressure. High-resolution transmission electron microscopy analysis of the saponite treated with octadecylammonium (n(C)=18) cations revealed the presence of 2:1 layer structures that have variable interlayer charge. The crystallization of these differently charged 2:1 layer silicates most likely occurred independently. The fact that 2:1 layer silicates with variable charge formed in the same gel has implications for our understanding of the origin of life, as these 2:1 clay minerals most likely replicate by a mechanism of template-catalyzed polymerization and transmit the charge distribution from layer to layer. If polar organic molecules like oxalate can catalyze the formation of clay-mineral crystals, which in turn promote clay microenvironments and provide abundant adsorption sites for other organic molecules present in solution, the interaction among these adsorbed molecules could lead to the polymerization of more complex organic molecules like RNA from nucleotides on early Earth.     

Microbial fuel cells applied to the metabolically based detection of extraterrestrial life

Since the 1970s, when the Viking spacecrafts carried out experiments to detect microbial metabolism on the surface of Mars, the search for nonspecific methods to detect life in situ has been one of the goals of astrobiology. It is usually required that a methodology detect life independently from its composition or form and that the chosen biological signature point to a feature common to all living systems, such as the presence of metabolism. In this paper, we evaluate the use of microbial fuel cells (MFCs) for the detection of microbial life in situ. MFCs are electrochemical devices originally developed as power electrical sources and can be described as fuel cells in which the anode is submerged in a medium that contains microorganisms. These microorganisms, as part of their metabolic process, oxidize organic material, releasing electrons that contribute to the electric current, which is therefore proportional to metabolic and other redox processes. We show that power and current density values measured in MFCs that use microorganism cultures or soil samples in the anode are much larger than those obtained with a medium free of microorganisms or sterilized soil samples, respectively. In particular, we found that this is true for extremophiles, which have been proposed as potential inhabitants of extraterrestrial environments. Therefore, our results show that MFCs have the potential to be used for in situ detection of microbial life.     

我要做怎样的新人

成都的秋夜,阵阵晚风透着好闻的清凉,一日的充实过后,我习惯走上楼顶,静赏黑夜的美丽。偶尔,一架夜航飞机打破夜的寂静,闪烁着美丽的夜航灯,满载着乘客飞往目的地。这短暂的轰鸣,让我的心却有着持续的共振……