Animal research facility for Space Station Freedom.

An integrated animal research facility is planned by NASA for Space Station Freedom which will permit long-term, man-tended experiments on the effects of space conditions on vertebrates. The key element in this facility is a standard type animal habitat which supports and maintains the animals under full bioisolation during transport and during the experiment. A holding unit accommodates the habitats with animals to be maintained at zero gravity; and a centrifuge, those to be maintained at artificial gravity for control purposes or for gravity threshold studies. A glovebox permits handling of the animals for experimental purposes and for transfer to a clean habitat. These facilities are described, and the aspects of environmental control, monitoring, and bioisolation are discussed.     

空间里的时间:微重力等环境下的生物节律研究

生物钟是地球上的生物为适应环境周期性变化经历长期演化而来的内在机制.在分子水平上受生物钟基因及其他相关基因的调节;在组织水平上,生物钟由主生物钟和外周生物钟组成.生物钟对于各种生物的生理、认知和行为等具有重要功能,是生物适应环境的决定因素之一.空间环境下的微重力、辐射、光照条件、社会性因素等与地面存在很大差异,这些因素均可能导致节律紊乱,影响生物的生理及环境适应性.因此,对地外生命的研究也应该考虑生物钟因素.对航天员而言,节律紊乱可引起睡眠障碍,并且对骨肌系统、神经系统、心血管系统及内分泌系统等造成不利影响,导致人的认知和工效水平下降.在未来空间生命探索以及航天员健康保障研究中,生物钟是一个不可忽视的重要因素.      

The role of gravity in the evolutionary emergence of multicellular complexity: Microgravity effects on arthropod development and aging

While experiments carried out in Space with isolated cells have shown that eucaryotic cells are able to sense and respond to the absence of gravity by modifying their reactions, experiments in which more complex processes have been investigated, such as Biological Systems undergoing development under Microgravity, have been surprisingly unaffected by the space environment. This can be considered a curious result since all organisms are evolutionarily adapted to the current level of the gravity force in our planet and should eventually change if this parameter will vary in a permanent manner. In fact, the small effects of the modifications in gravity on development in short term experiments may be equivalent to the difficulties in detecting the involvement of other basic physical processes such as diffusion-controled auto-organizative reactions in currently developing biological systems. An apparent exception to this lack of effect is experiments where brine shrimp dormant gastrulae directly exposed to the space environment accumulate developmental defects as a consequence of cosmic irradiation. In this article we discuss the idea that at a certain stage during the evolutionary emergence of multicellular organisms the cues laid by generic forces such as gravity were involved in the evolutionary organization of these primitive organisms. As evolution proceed, these early mechanisms may have been obscured and/or made redundant by the appearance of new internal, environment-independent biological regulatory mechanisms. On the other hand, behavioral responses that may be important, for example, in setting the life-spans of organisms may still be more readily susceptible to manipulation by external cues as experiments carried out by our group in Space and on the ground with Drosophila melanogaster indicate.     

Exploration life support technology challenges for the Crew Exploration Vehicle and future human missions

As NASA implements the U.S. Space Exploration Policy, life support systems must be provided for an expanding sequence of exploration missions. NASA has implemented effective life support for Apollo, the Space Shuttle, and the International Space Station (ISS) and continues to develop advanced systems. This paper provides an overview of life support requirements, previously implemented systems, and new technologies being developed by the Exploration Life Support Project for the Orion Crew Exploration Vehicle (CEV) and Lunar Outpost and future Mars missions. The two contrasting practical approaches to providing space life support are (1) open loop direct supply of atmosphere, water, and food, and (2) physicochemical regeneration of air and water with direct supply of food. Open loop direct supply of air and water is cost effective for short missions, but recycling oxygen and water saves costly launch mass on longer missions. Because of the short CEV mission durations, the CEV life support system will be open loop as in Apollo and Space Shuttle. New life support technologies for CEV that address identified shortcomings of existing systems are discussed. Because both ISS and Lunar Outpost have a planned 10-year operational life, the Lunar Outpost life support system should be regenerative like that for ISS and it could utilize technologies similar to ISS. The Lunar Outpost life support system, however, should be extensively redesigned to reduce mass, power, and volume, to improve reliability and incorporate lessons learned, and to take advantage of technology advances over the last 20 years. The Lunar Outpost design could also take advantage of partial gravity and lunar resources.     

Influence of gravity on the circadian timing system.

The circadian timing system (CTS) is responsible for daily temporal coordination of physiological and behavioral functions both internally and with the external environment. Experiments in altered gravitational environments have revealed changes in circadian rhythms of species ranging from fungi to primates. The altered gravitational environments examined included both the microgravity environment of spaceflight and hyperdynamic environments produced by centrifugation. Acute exposure to altered gravitational environments changed homeostatic parameters such as body temperature. These changes were time of day dependent. Exposure to gravitational alterations of relatively short duration produced changes in both the homeostatic level and the amplitude of circadian rhythms. Chronic exposure to a non-earth level of gravity resulted in changes in the period of the expressed rhythms as well as in the phase relationships between the rhythms and between the rhythms and the external environment. In addition, alterations in gravity appeared to act as a time cue for the CTS. Altered gravity also affected the sensitivity of the pacemaker to other aspects of the environment (i.e., light) and to shifts of time cues. Taken together, these studies lead to the conclusion that the CTS is indeed sensitive to gravity and its alterations. This finding has implications for both basic biology and space medicine.     

相变材料容器的瞬态热分析

空间太阳能热动力发电系统是很有前途的空间电源系统。基于微重力状态下的导热控制微分方程，采用焓法对相变材料容器进行了二维数值分析，改进了以往的模拟计算，对计算结果给予了讨论。     

Gravitational response of the slime mold Physarum.

The acellular slime mold Physarum polycephalum is used as a model system to investigate the graviresponse of single cells which possess no receptors specialized for the perception of gravity. To obtain insights into the gravity-signal transduction mechanism the light response of the cell is used: Macroplasmodia of the slime mold show clear geo- and phototaxes. Gravity increases and white light decreases transiently the contraction frequency of plasmodial strands whereby both responses follow the same time pattern. Since mitochondria play a major role in changing the contraction rhythm in response to light and gravity stimuli, the simultaneous and subsequent inductions of the opposing light and gravity responses and their mutual influences on one another were investigated. The experiments were performed in weightlessness (0 g)--simulated on the fast-rotating clinostat as well as in actual weightlessness during the IML-1 Space Shuttle mission. The results indicate that mitochondria (chondriome) are part of the acceleration-stimulus reaction chain in Physarum. Two models for a direct gravireceptor mechanism are discussed.     

Chromosomes and plant cell division in space: environmental conditions and experimental details.

Details of the plant cultivation system developed for the CHROMEX experiment flown aboard the Shuttle Discovery (March, 1989) in NASA's Plant Growth Unit (PGU) are presented. The physical regime as measured during Spaceflight, both within the orbiter cabin environment and within the PGU itself, is discussed. These data function as a guide to what may be representative of the environmental regime in which Space-based plant cultivation systems will be operating, at least for the near-term. Attention is also given to practical considerations involved in conducting a plant experiment in Space. Of particular importance are the differences expected to occur in moisture distribution patterns within substrates used to cultivate plants in Space vs on Earth.     

Crickets in space: morphological, physiological and behavioral alterations induced by space flight and hypergravity.

"Crickets in Space" was a Neurolab experiment by which the balance between genetic programs and the gravitational environment for the development of a gravity sensitive neuronal system was studied. The model character of crickets was justified by their external gravity receptors, identified position-sensitive interneurons (PSI) and gravity-related compensatory head response, and by the specific relation of this behavior to neuronal arousal systems activated by locomotion. These advantages allowed to study the impact of modified gravity on cellular processes in a complex organism. Eggs, 1st, 4th and 6th stage larvae of Acheta domesticus were used. Post-flight experiments revealed a low susceptibility of the behavior to micro- and hypergravity while the physiology of the PSI was significantly affected. Immunocytological investigations revealed a stage-dependent sensitivity of thoracic GABAergic motoneurons to 3 g-conditions concerning their soma sizes but not their topographical arrangement. The morphology of neuromuscular junctions was not affected by 3 g-hypergravity. Peptidergic neurons from cerebral sensorimotor centers revealed no significant modifications by microgravity (micro g). The contrary physiological and behavioral results indicate a facilitation of 1 g-readaptation originating from accessory gravity, proprioceptive and visual sense organs. Absence of anatomical modifications point to an effective time window of micro g or 3 g-expo-sure related to the period of neuronal proliferation. The analysis of basic mechanisms of how animals and man adapt to altered gravitational conditions will profit from a continuation of the project "Crickets in Space".     

Arthropod model systems for studying complex biological processes in the space environment.

Three arthropod systems are discussed in relation to their complementary and potential use in Space Biology. In a next biosatellite flight, Drosophila melanogaster pre-adapted during several months to different g levels will be flown in an automatic device that separates parental from first and second generations. In the same flight, flies will be exposed to microgravity conditions in an automatic unit in which fly motility can be recorded. In the International Microgravity Laboratory-2, several groups of Drosophila embryos will be grown in Space and the motility of a male fly population will be video-recorded. In the Biopan, an ESA exobiology facility that can be flown attached to the exterior of a Russian biosatellite, Artemia dormant gastrulae will be exposed to the space environment in the exterior of the satellite under a normal atmosphere or in the void. Gastrulae will be separated in hit and non-hit populations. The developmental and aging response of these animals will be studied upon recovery. With these experiments we will be able to establish whether exposure to the space environment influences arthropod development and aging, and elaborate on some of the cellular mechanisms involved which should be tested in future experiments.     

Classification of gravity effects on “free” cells

When cell physiologists detect gravity related reactions of their objects it is often difficult to decide where the receptors for the observed effects are located. Answering this question is necessary for any further analysis of a detected gravity effect on cells. In previous papers we have discussed direct and indirect gravity effects in relation to the smallest functional units where the primary receptor, which interacts with gravity, is positioned inside and outside of such a unit, respectively. So, in a first approximation we can conclude that in a multicellular aquatic organism, which changes its metabolism in weightlessness, the primary receptors of gravity are located inside the cells of that organism. A special approach is necessary when free living cells, the density of which may be higher than the one of the (liquid) medium, or even cells living on a free surface are observed. In these two cases also indirect effects have to be taken into account, which will be demonstrated with the aid of the slime mold . Additionally the environment of the organisms can be changed directly and indirectly by gravity.     

Gravity model development for precise orbit computations for satellite altimetry

Satellite altimeters have been developed which have the capability of measuring the surface topography of the ocean over entire basins for time periods of several years with a precision of a few cm. The combination of these data with accurate satellite position information, subsurface measurements and models of the density field can be used to determine the general circulation of the ocean and its variability. The Topex altimeter oceanographic experiment proposed for the early 1990's requires a radial orbit accuracy approaching 10 cm. Considerable progress has been made at the Goddard Space Flight Center in developing precision orbit determination to support satellite altimetry analysis. This progress is attributed to the development of more accurate models of the earth gravity field, earth and ocean tidal effects, a reference coordinate system as well as improved drag and solar radiation pressure computations. This computational system has been used for the Seasat data acquired during the 1978 time period and has produced an rms radial accuracy of about 50 cm. A first step towards the development of a new Interim gravity model is presented. Global orbit determination accuracies have been improved using these new PGS-T1 and T2 fields.     

Development of the Infrared Telescope in Space: IRTS

Infrared Telescope in Space (IRTS) is a cryogenically cooled small infrared telescope aboard the small space platform, SFU (Space Flyer Unit). IRTS has a telescope of 15 cm diameter, which is rather small compared with other big space missions. IRTS, however, is optimized to observe extended diffuse sources with four focal plane instruments which have wide wavelength coverage from the near infrared to the submillimeter region.

All instruments have been calibrated with a test cryostat, and integrated into the flight cryostat. Cooling tests of the whole IRTS system have confirmed that instruments and cryostat will perform as expected. Integration of the IRTS on the SFU will soon begin and the ground tests of the SFU system will be conducted in the coming year.

The launch of the SFU will be in early 1995. The IRTS will survey the 10% of the sky during the three week mission and provide significant scientific results on cosmology, galactic structure, interstellar matter, stars, and solar system.     

Issues of exploration: human health and wellbeing during a mission to Mars.

Today, the tools are in our hands to enable us to travel away from our home planet and become citizens of the solar system. Even now, we are seriously beginning to develop the robust infrastructure that will make the 21st century the Century of Space Travel. But this bold step must be taken with due concern for the health, safety and wellbeing of future space explorers. Our long experience with space biomedical research convinces us that, if we are to deal effectively with the medical and biomedical issues of exploration, then dramatic and bold steps are also necessary in this field. We can no longer treat the human body as if it were composed of muscles, bones, heart and brain acting independently. Instead, we must lead the effort to develop a fully integrated view of the body, with all parts connected and fully interacting in a realistic way. This paper will present the status of current (2000) plans by the National Space Biomedical Research Institute to initiate research in this area of integrative physiology and medicine. Specifically, three example projects are discussed as potential stepping stones towards the ultimate goal of producing a digital human. These projects relate to developing a functional model of the human musculoskeletal system and the heart.     

Mapping the earth's magnetic and gravity fields from space: Current status and future prospects

Orbital potential field measurements are sensitive to regional variations in earth density and magnetization that occur over scales of a few hundred kilometers or greater. Global field models currently available are able to distinguish gravity variations of ±5 milligal over distances of ～1,000 km and magnetic variations of ±6 gamma over distances of ～300 km at the earth's surface. Regional variations in field strength have been detected in orbital measurements that are not apparent in higher resolution, low altitude surveys. NASA is presently studying a spacecraft mission known as GRAVSAT/MAGSAT, which would be the first satellite mission to perform a simultaneous survey of the earth's gravity and magnetic fields at low orbital altitudes. GRAVSAT/MAGSAT has been proposed for launch during the latter nineteen-eighties, and it would measure gravity field strength to an accuracy of 1 milligal and magnetic field strength to an accuracy of 2 gamma (scalar)/5 gamma (vector components) over a distance of roughly 100 km. Even greater improvements in the accuracy and spatial resolution of orbital surveys are anticipated during the nineteen-nineties with the development of potential field gradiometers and a tethered satellite system that can be deployed from the Space Shuttle to altitudes of 120 km above the earth's surface.     

Early development in the mouse: would it be affected by microgravity?

Gravity has been identified as a morphogenetic signal in Amphibian and bird embryonic development so it is plausible that it might be such in mammals as well. Since early mammalian development shows some apparently significant differences to these other groups, a brief summary of mouse embryogenesis will be given identifying events in which polarity is an important feature and consequently, in which gravity may be a causative factor. These include compaction and polarization during cleavage, establishment of the radial axis, the embryonic-abembryonic axis, the dorso-ventral axis, and the anterior-posterior axis, implantation, and the later rotation of the embryo. The experimental data on these morphogenetic steps will be discussed and an assessment of the possible involvement of gravity will be made.     

一种全天空气辉成像仪数据预处理方法和初步结果

大气重力波是大气中的基本波动形式之一,在中高层大气动力和热力学过程中起着十分重要的作用.全天空气辉成像仪是一种以大气气辉辐射为示踪物,能够有效对大气重力波成像的仪器.本文针对中国科学院国家空间科学中心空间天气学国家重点实验室中高层大气组自主研制的全天空气辉成像仪所观测的数据,提出了一种气辉图像预处理方法,进行平场校正、方位校正、星光去除和坐标映射等数据订正.利用该方法处理2015年5月17日21:00BLT至次日05:00BLT西宁台站（36.6°N,101.7°E）的OH气辉数据,发现一次重力波事件,分析并获得了该重力波的水平波长、观测水平相速度和传播方向（分别为17.72km,47m·s-1,339°）.研究结果表明该方法是可行的.      

Fertilization and development of eggs of the South African clawed toad, Xenopus laevis, on sounding rockets in space.

Egg rotation and centrifugation experiments strongly suggest a role for gravity in the determination of the spatial structure of amphibian embryos. Decisive experiments can only be made in Space. Eggs of Xenopus laevis, the South African clawed toad, were the first vertebrate eggs which were successfully fertilized on Sounding Rockets in Space. Unfixed, newly fertilized eggs survived reentry, and a reasonable number showed a seemingly normal gastrulation but died between gastrulation and neurulation. Only a few reached the larval stage, but these developed abnormally. In the future, we intend to test whether this abnormal morphogenesis is due to reentry perturbations, or due to a real microgravity effect, through perturbation of the reinitiation of meiosis and other processes, or started by later sperm penetration.     

An overview of Japanese CELSS research activities.

Many research activities regarding Controlled Ecological Life Support System (CELSS) have been conducted and continued all over the world since the 1960's and the concept of CELSS is now changing from Science Fiction to Scientific Reality. Development of CELSS technology is inevitable for future long duration stays of human beings in space, for lunar base construction and for manned mars flight programs. CELSS functions can be divided into two categories, Environment Control and Material Recycling. Temperature, humidity, total atmospheric pressure and partial pressure of oxygen and carbon dioxide, necessary for all living things, are to be controlled by the environment control function. This function can be performed by technologies already developed and used as the Environment Control Life Support System (ECLSS) of Space Shuttle and Space Station. As for material recycling, matured technologies have not yet been established for fully satisfying the specific metabolic requirements of each living thing including human beings. Therefore, research activities for establishing CELSS technology should be focused on material recycling technologies using biological systems such as plants and animals and physico-chemical systems, for example, a gas recycling system, a water purifying and recycling system and a waste management system. Based on these considerations, Japanese research activities have been conducted and will be continued under the tentative guideline of CELSS research activities as shown in documents /1/, /2/. The status of the over all activities are discussed in this paper.