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1.
The lipid peroxidation (LP) intensity and the activity of the antioxidant enzyme superoxide dismutase (SOD) were studied in chloroplasts of pea (Pisum sativum L.) plants grown for 7 and 14 days under clino-rotation. An increase in LP levels in chloroplasts during both terms of clinorotation in comparison with stationary controls was documented. SOD activity increased in chloroplasts of plants that were clino-rotated for seven days. SOD has a significant protective effect by diminishing the availability of O2-. However, under more prolonged clino-rotation (14 days), SOD activity decreased but was still higher than in the control samples. In accordance with Selye's oxidative stress theory (Selye, 1956; modified by Leshem et al., 1998), plants that were clino-rotated for seven days are presumed to be in a stage of resistance while 14-day plants reached a stage of exhaustion.  相似文献   

2.
Formation and structure of potato minitubers grown aseptically for 30 days on a horizontal clinostat and in stationary control have been studied by light and electron microscopy. It was demonstrated that the number of plants that formed minitubers, their size and fresh weight, was higher when clino-rotated than in the stationary control. It was revealed that the amount of amyloplasts in parenchyma cell sections was doubled in minitubers formed under clino-rotation. Other factors (shape of minitubers and size of reserve parenchyma cells) did not differ from the stationary control. The changes in amyloplast ultrastructure suggest accelerated cell maturity of potato reserve parenchyma in extended clino-rotation.  相似文献   

3.
Results from experiments that used cells from the unicellular alga Chlorella vulgaris (strain Larg-1) grown on a clinostat, demonstrated the occurrence of rearrangements in cellular organelles, including changes in the mitochondrial ultrastructure compared to controls. Changes in mitochondrial structure were observed in auto- and heterotrophic regimes of cells grown in altered gravity conditions, especially in long-term experiments. The mitochondrial rearrangements become apparent during cell proliferation, which resulted in an increase in the relative volume of mitochondria per cell: up to 2.7 +/- 0.3% in short-term clino-rotation (2.2 +/- 0.1% in the control) and up to 5.3 +/- 0.4% and 5.1 +/- 0.4% in long-term clinorotation (2.3 +/- 0.2% in the control). The size of the mitochondria and their cristae increased in cells grown under long-time clinorotation. In addition, hypertrophied organelles, not typical for this strain, were observed. These changes in the cells were accompanied by increased electron density of the matrix and a well-ordered topography of the cristae. To examine the separation of oxidative phosphorylation and respiration, an inhibitory agent 2,4-dinitrophenol (2,4-DNP) was applied to cells which resulted in insignificant volume changes of the mitochondria (2.5 +/- 0.4% versus 2.1 +/- 0.2% in the control). The increase of mitochondrial size with regularly arranged cristae, with more condensed matrix and extension of cristae areas of clino-rotated cells, may demonstrate higher functional activity of the mitochondria under altered gravity conditions. Changes observed early in clinorotated cells, in particular the increased level of respiration, adenylate content (especially ATP) and more intensive electron-cytochemical reactions of Mg2(+)-ATPase and succinate [correction of succinat] dehydrogenase (SDH) in mitochondria (including hypertrophic organelles), also suggest increased activity of mitochondria from cells grown under altered gravity conditions compared to controls.  相似文献   

4.
Information about compositional changes in plants grown in controlled environments is essential for developing a safe, nutritious diet for a Controlled Ecomological Life-Support System (CELSS). Information now is available for some CELSS candidate crops, but detailed information has been lacking for soybeans. To determine the effect of environment on macronutrient and mineral composition of soybeans, plants were grown both in the field and in a controlled environment where the hydroponic nutrient solution, photosynthetic flux (PPF), and CO2 level were manipulated to achieve rapid growth rates. Plants were harvested at seed maturity, separated into discrete parts, and oven dried prior to chemical analysis. Plant material was analyzed for proximate composition (moisture, protein, lipid, ash, and carbohydrate), total nitrogen (N), nonprotein N (NPN), nitrate, minerals, amino acid composition, and total dietary fiber. The effect of environment on composition varied by cultivar and plant part. Chamber-grown plants generally exhibited the following characteristics compared with field-grown plants: 1) increased total N and protein N for all plant parts, 2) increased nitrate in leaves and stems but not in seeds, 3) increased lipids in seeds, and 4) decreased Ca:P ratio for stems, pods, and leaves. These trends are consistent with data for other CELSS crops. Total N, protein N, and amino acid contents for 350 ppm CO2 and 1000 ppm CO2 were similar for seeds, but protein N and amino acid contents for leaves were higher at 350 ppm CO2 than at 1000 ppm CO2. Total dietary fiber content of soybean leaves was higher with 350 ppm CO2 than with 1000 ppm CO2. Such data will help in selecting of crop species, cultivars, and growing conditions to ensure safe, nutritious diets for CELSS.  相似文献   

5.
Proton fluxes obtained by two instruments carried on the ESA/NASA Ulysses spacecraft are reported for the period from launch in October 1990 till Jupiter encounter in February 1992. Proton energy ranges are 24-59, 71-99, 130-320, 320-2100 and > 2100 MeV. The Sun was very active during this period, the events of March 1991 being some of the largest of the solar cycle. The relationship between events on the Sun and the observed proton flux is discussed.  相似文献   

6.
Composition and physical properties of starch in microgravity-grown plants.   总被引:1,自引:0,他引:1  
The effect of spaceflight on starch development in soybean (Glycine max L., BRIC-03) and potato (Solanum tuberosum, Astroculture-05) was compared with ground controls by biophysical and biochemical measurements. Starch grains from plants from both flights were on average 20-50% smaller in diameter than ground controls. The ratio delta X/delta rho (delta X --difference of magnetic susceptibilities, delta rho--difference of densities between starch and water) of starch grains was ca. 15% and 4% higher for space-grown soybean cotyledons and potato tubers, respectively, than in corresponding ground controls. Since the densities of particles were similar for all samples (1.36 to 1.38 g/cm3), the observed difference in delta X/delta rho was due to different magnetic susceptibilities and indicates modified composition of starch grains. In starch preparations from soybean cotyledons (BRIC-03) subjected to controlled enzymatic degradation with alpha-amylase for 24 hours, 77 +/- 6% of the starch from the flight cotyledons was degraded compared to 58 +/- 12% in ground controls. The amylose content in starch was also higher in space-grown tissues. The good correlation between the amylose content and delta X/delta rho suggests, that the magnetic susceptibility of starch grains is related to their amylose content. Since the seedlings from the BRIC-03 experiment showed elevated post-flight ethylene levels, material from another flight experiment (GENEX) which had normal levels of ethylene was examined and showed no difference to ground controls in size distribution, density, delta X/delta rho and amylose content. Therefore the role of ethylene appears to be more important for changes in starch metabolism than microgravity.  相似文献   

7.
通过分析GOES和ACE卫星大于10MeV能量段的5min平均质子通量数据,发现两者有很好的相关性,最佳相关系数显示,GOES通量数据较ACE卫星数据有数十分钟至几小时的时间延迟.这为利用ACE数据进行质子事件预警提供了依据.本文提出一种利用ACE卫星大于10MeV能量段的通量数据对质子事件进行预警的方法.该方法在2001年8月7日至2012年2月20日这11年多时间的试验中,表现出较高的报准率(76.3%)和较低的虚报率(14.7%),预警提前时间在数十分钟至几小时不等.该方法的结果与美国国家海洋和大气管理局(NOAA)空间天气预测中心(SWPC)使用的预警模型对比,具有更高的报准率,较低的虚报率,在预警时间提前量方面两者相当.   相似文献   

8.
We present an analysis of the ionosphere and thermosphere response to Solar Proton Events (SPE) and magnetospheric proton precipitation in January 2005, which was carried out using the model of the entire atmosphere EAGLE. The ionization rates for the considered period were acquired from the AIMOS (Atmospheric Ionization Module Osnabrück) dataset. For numerical experiments, we applied only the proton-induced ionization rates of that period, while all the other model input parameters, including the electron precipitations, corresponded to the quiet conditions. In January 2005, two major solar proton events with different energy spectra and proton fluxes occurred on January 17 and January 20. Since two geomagnetic storms and several sub-storms took place during the considered period, not only solar protons but also less energetic magnetospheric protons contributed to the calculated ionization rates. Despite the relative transparency of the thermosphere for high-energy protons, an ionospheric response to the SPE and proton precipitation from the magnetotail was obtained in numerical experiments. In the ionospheric E layer, the maximum increase in the electron concentration is localized at high latitudes, and at heights of the ionospheric F2 layer, the positive perturbations were formed in the near-equatorial region. An analysis of the model-derived results showed that changes in the ionospheric F2 layer were caused by a change in the neutral composition of the thermosphere. We found that in the recovery phase after both solar proton events and the enhancement of magnetospheric proton precipitations associated with geomagnetic disturbances, the TEC and electron density in the F region and in topside ionosphere/plasmasphere increase at low- and mid-latitudes due to an enhancement of atomic oxygen concentration. Our results demonstrate an important role of magnetospheric protons in the formation of negative F-region ionospheric storms. According to our results, the topside ionosphere/plasmasphere and bottom-side ionosphere can react to solar and magnetospheric protons both with the same sign of disturbances or in different way. The same statement is true for TEC and foF2 disturbances. Different disturbances of foF2 and TEC at high and low latitudes can be explained by topside electron temperature disturbances.  相似文献   

9.
采用GOSE-10卫星4~9 MeV(P2),9~15 MeV(P3),15~40 MeV(P4),40~80 MeV(P5)能段上的质子通量数据,结合质子能谱,对太阳质子事件发生前各能谱参数的变化特征进行分析,详细介绍利用能谱参数的变化特征及能量E>10 MeV的质子通量数据对太阳质子事件进行预报的新方法,并运用这种方法对2002-2006年期间太阳质子事件进行了预报.预报结果显示,预报提前量最多达到100 h以上,对质子事件的报准率达97.5%,预报方法具备一定的有效性和实用性.   相似文献   

10.
太阳质子事件对航天活动构成重要威胁, 预测一定时期内太阳质子通量对航天器抗辐射加固设计有重要的指导意义. 在第20至23太阳活动周的太阳质子事件数据统计分析的基础上, 建立了一个针对 E>10 MeV和 E>30 MeV太阳质子通量的新模型. 新模型与目前航天工程中常用的JPL模型相比较, 引入了太阳活动性对质子事件发生概率的影响因素, 能够评估不同太阳活动水平下的质子通量, 其结果更符合质子事件的分布特征.   相似文献   

11.
    
免疫应答反应一直是限制可植入生物材料应用的关键因素之一。实验评估了2种用于骨修复的胶原膜在体内的免疫反应,希望为其临床试验提供依据。在将2种膜皮下植入到BALB/c小鼠后的第14天,与阴性对照(NC,未植入材料)相比,脾和淋巴结没有明显肿大,淋巴结细胞数几乎没差异,而脾细胞数大约是NC的2倍。流式细胞术分析显示植入胶原膜1导致脾中T细胞比例减少了约13%,但是没有影响T细胞亚群,而植入胶原膜2对小鼠的脾细胞组成没有明显影响;2种胶原膜都激活了一定的B细胞,激活率大约为NC小鼠的2倍。淋巴细胞体外增殖实验显示与NC组无显著差异。酶联免疫吸附测试表明胶原膜1导致了第14天血清中的抗体浓度升高至NC小鼠的2倍。局部H&E染色显示2种材料都引起了轻微的细胞浸润。这2种胶原膜引起的免疫反应很微弱,可以被应用于临床试验。  相似文献   

12.
Recent developments in NASA’s deterministic High charge (Z) and Energy TRaNsport (HZETRN) code have included lateral broadening of primary ion beams due to small-angle multiple Coulomb scattering, and coupling of the ion-nuclear scattering interactions with energy loss and straggling. This new version of HZETRN is based on Green function methods, called GRNTRN, and is suitable for modeling transport with both space environment and laboratory boundary conditions. Multiple scattering processes are a necessary extension to GRNTRN in order to accurately model ion beam experiments, to simulate the physical and biological-effective radiation dose, and to develop new methods and strategies for light-ion radiation therapy. In this paper we compare GRNTRN simulations of proton lateral broadening distributions with beam measurements taken at Loma Linda University Proton Therapy Facility. The simulated and measured lateral broadening distributions are compared for a 250 MeV proton beam on aluminum, polyethylene, polystyrene, bone substitute, iron, and lead target materials. The GRNTRN results are also compared to simulations from the Monte Carlo MCNPX code for the same projectile-target combinations described above.  相似文献   

13.
介绍了南大西洋异常区的辐射环境及其特点,重点研究了发生于2000年7月14日的太阳质子事件对太阳同步轨道空间环境造成的影响,太阳质子事件期间,抵达近地空间的高能电子、质子及重离子对太阳同步轨道空间环境造成剧烈地扰动,并且不同种类不同能量的粒子扰动特征不尽相同。  相似文献   

14.
Variations in the Earth's trapped (Van Allen) belts produced by solar flare particle events are not well understood. Few observations of increases in particle populations have been reported. This is particularly true for effects in low Earth orbit, where manned spaceflights are conducted. This paper reports the existence of a second proton belt and it's subsequent decay as measured by a tissue-equivalent proportional counter and a particle spectrometer on five Space Shuttle flights covering an eighteen-month period. The creation of this second belt is attributed to the injection of particles from a solar particle event which occurred at 2246 UT, March 22, 1991. Comparisons with observations onboard the Russian Mir space station and other unmanned satellites are made. Shuttle measurements and data from other spacecraft are used to determine that the e-folding time of the peak of the second proton belt. It was ten months. Proton populations in the second belt returned to values of quiescent times within eighteen months. The increase in absorbed dose attributed to protons in the second belt was approximately 20%. Passive dosimeter measurements were in good agreement with this value.  相似文献   

15.
Atmospheric carbon-dioxide enrichment is known to affect the yield of lettuce and radish grown in controlled environments, but little is known about CO2 enrichment effects on the chemical composition of lettuce and radish. These crops are useful model systems for a Controlled Ecological Life-Support System (CELSS), largely because of their relatively short production cycles. Lettuce (Lactuca sativa L.) cultivar 'Waldmann's Green' and radish (Raphanus sativus L.) cultivar 'Giant White Globe' were grown both in the field and in controlled environments, where hydroponic nutrient solution, light, and temperature were regulated, and where CO2 levels were controlled at 400, 1000, 5000, or 10,000 ppm. Plants were harvested at maturity, dried, and analyzed for proximate composition (protein, fat, ash, and carbohydrate), total nitrogen (N), nitrate N, free sugars, starch, total dietary fiber, and minerals. Total N, protein N, nonprotein N (NPN), and nitrate N generally increased for radish roots and lettuce leaves when grown under growth chamber conditions compared to field conditions. The nitrate-N level of lettuce leaves, as a percentage of total NPN, decreased with increasing levels of CO2 enrichment. The ash content of radish roots and of radish and lettuce leaves decreased with increasing levels of CO2 enrichment. The levels of certain minerals differed between field- and chamber-grown materials, including changes in the calcium (Ca) and phosphorus (P) contents of radish and lettuce leaves, resulting in reduced Ca/P ratio for chamber-grown materials. The free-sugar contents were similar between the field and chamber-grown lettuce leaves, but total dietary fiber content was much higher in the field-grown plant material. The starch content of growth-chamber lettuce increased with CO2 level.  相似文献   

16.
Activity of auxin polar transport in inflorescence axes of Arabidopsis thaliana grown under simulated microgravity conditions was studied in relation to the growth and development. Seeds were germinated and allowed to grow on an agar medium in test tubes on a horizontal clinostat. Horizontal clinostat rotation substantially reduced the growth of inflorescence axes and the productivity of seeds of Arabidopsis thaliana (ecotypes Landsberg erecta and Columbia), although it little affected seed germination, development of rosette leaves and flowering. The activity of auxin polar transport in inflorescence axes decreased when Arabidopsis plants were grown on a horizontal clinostat from germination stage, being ca. 60% of 1 g control. On the other hand, the auxin polar transport in inflorescence axes of Arabidopsis grown in 1 g conditions was not affected when the segments were exposed to various gravistimuli, including 3-dimensional clinorotation, during transport experiments. Pin-formed mutant of Arabidopsis, having a unique structure of the inflorescence axis with no flower and extremely low levels of the activity of auxin polar transport in inflorescence axes and endogenous auxin, did not continue its vegetative growth under clinostat rotation. These facts suggest that the development of the system of auxin polar transport in Arabidopsis is affected by microgravity, resulting in the inhibition of growth and development, especially during reproductive growth.  相似文献   

17.
Two CELSS candidate crops, soybean (Glycine max) and potato (Solanum tuberosum), were grown hydroponically in controlled environments maintained at carbon dioxide (CO2) partial pressures ranging from 0.05 to 1.00 kPa (500 to 10,000 ppm at 101 kPa atmospheric pressure). Plants were harvested at maturity (90 days for soybean and 105 days for potato) and all tissues analyzed for proximate nutritional composition (i.e. protein, fat, carbohydrate, crude fiber, and ash content). Soybean seed ash and crude fiber were higher and carbohydrate was lower than values reported for field-grown seed. Potato tubers showed little difference from field-grown tubers. With the exception of increased crude fiber of soybean seed with increased CO2, no trends were apparent with regard to CO2 effects on proximate composition of soybean seed and potato tubers. Crude fiber of soybean stems and leaves increased with increased CO2, as did soybean leaf protein (total nitrogen). Potato leaf and stem (combined) protein levels also increased with increased CO2, while leaf and stem carbohydrates decreased. Values for leaf and stem protein and ash were higher than values generally reported for field-grown plants for both species. Results suggest that CO2 partial pressure should have little influence on proximate composition of potato tubers or soybean seed, but that high ash and protein levels might be expected from leaves and stems of crops grown in controlled environments of a CELSS.  相似文献   

18.
建立由太阳光球磁场和日冕偏振亮度等观测约束的单流体太阳风模型,包括日冕和太阳风的等离子体密度、速度和磁场,温度还有待于以后处理.这里采用高山观测台(HAO)MKⅢ的日冕偏振亮度(pB)在1.36Rs上的观测概图,根据Guhathakurta在1996年发展的日冕电子密度反演模型确定日冕的电子密度分布.同时采用Wilcox太阳观测台(WSO)的光球磁场视向分量的观测概图作为底部边界,根据Zhao等在1994年发展的水平电流-电流片(HCCS)模型得到全球磁场.Phillips在1995年及McComas在2003年分别用Ulysses第一次和第二次跨极飞行的观测发现,归一化到1 AU的太阳风动量流密度除了在10°~30°的纬度范围内略低以外几乎不变.根据这一结论,结合已经得到的密度数据,就可以得到日冕和太阳风的速度.将上面的模型应用于1918卡林顿自转周稳态太阳风的研究,结果与太阳活动极小期的观测基本相符,但是与观测相比较低速高密度区偏大,因此密度模型还有待改进.   相似文献   

19.
Changes in the ultrastructure of protonema cells of Funaria hygrometrica, cultivated during 20 days on a horizontal clinostat (2 rev/min), were determined by the electron microscopy method. About 20% of the cells were almost identical to those in the control, 20% were destructive cells, and in 60% ultrastructure changes were observed. The heterogeneity of the reaction demonstrated the evidence of sensitive cells on the clinostation process. Changes affected the ultrastructure of plastids, wall of the cell, and the form of the nucleus as well. Starch disappeared from chloroplasts practically completely, thylakoids swelled, granas frequently disappeared from plastids. Peroxisomes number in cells increased appreciably, width of cell walls decreased by almost half their size. Ca++-binding sites were revealed in cytoplasma of cells. Electronocytochemical exposure of ATPases activity with the presence of Mg++ and Ca++ ions showed that Mg2+-ATPase activity localization in clinosted cells was not too different from the control, while Ca2+-ATPase location presented differences in plasmalemma and Ca-sites. These changes are perhaps connected with the membranes permeability breaking and affect the plant cells adaptation to the influence of hypogravitation.  相似文献   

20.
In low earth orbit, the SAA region is the dominant contributor to both proton environment and electron environment from the standpoint of radiation dose for spacecraft lifetime. However, the polar region and the horn region are sometimes strongly disturbed due to large solar and geomagnetic events. During large disturbances, enhancements in proton flux are measured in the polar region, which gives temporary more severe space radiation environment than that given in the SAA region. On the other hand, enhancements in electron flux are measured mainly in the horn region corresponding to the outer radiation belt, which are likely sources of high-energy electrons in the inner radiation belt. These short-term disturbances have another radiation hazard to spacecraft such as single event and electrostatic discharge.  相似文献   

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