Biophysical effect of cosmic heavy ions of distinct LET-classes in a plant model system.

Results presented from recent space flight BION 9 show biological effects of different LET-classes of HZE-particles in different target regions of the seed (meristem and the whole embryo) of Arabidopsis thaliana (L.) Heyhn. HZE-one hit events and non-hit events, i.e. only hit by the low-LET background radiation, and their combined effects on the biological damage endpoint lethality are distinguished. This procedure is opening the opportunity of an approach to comparative studies of the biological effects induced by cosmic HZE-particles of different LET-ranges interacting in the complex cosmic radiation spectrum and with other space flight conditions.     

Inactivation of individual Bacillus subtilis spores in dependence on their distance to single cosmic heavy ions.

For radiobiological experiments in space, designed to investigate biological effects of the heavy ions of the cosmic radiation field, a mandatory requirement is the possibility to spatially correlate the observed biological response of individual test organisms to the passage of single heavy ions. Among several undertakings towards this goal, the BIOSTACK experiments in the Apollo missions achieved the highest precision and therefore the most detailed information on this question. Spores of Bacillus subtilis as a highly radiation resistant and microscopically small test organism yielded these quantitative results. This paper will focus on experimental and procedural details, which must be included for an interpretation and a discussion of these findings in comparison to control experiments with accelerated heavy ions.     

Particle trajectories in seeds of Lactuca sativa and chromosome aberrations after exposure to cosmic heavy ions on Cosmos Biosatellites 8 and 9.

The potentially specific importance of the heavy ions of the galactic cosmic radiation for radiation protection in manned spaceflight continues to stimulate in situ, i.e., spaceflight experiments to investigate their radiobiological properties. Chromosome aberrations as an expression of a direct assault on the genome are of particular interest in view of cancerogenesis being the primary radiation risk for man in space. In such investigations the establishment of the geometrical correlation between heavy ions' trajectories and the location of radiation sensitive biological substructures is an essential task. The overall qualitative and quantitative precision achieved for the identification of particle trajectories in the order of approximately 10 micrometers as well as the contributing sources of uncertainties are discussed. We describe how this was achieved for seeds of Lactuca sativa as biological test organisms, whose location and orientation had to be derived from contact photographies displaying their outlines and those of the holder plates only. The incidence of chromosome aberrations in cells exposed during the COSMOS 1887 (Biosatellite 8) and the COSMOS 2044 (Biosatellite 9) mission was determined for seeds hit by cosmic heavy ions. In those seeds the incidence of both single and multiple chromosome aberrations was enhanced. The results of the Biosatellite 9 experiment, however, are confounded by spaceflight effects unrelated to the passage of heavy ions.     

Simultaneous exposure of mammalian cells to heavy ions and X-rays.

Crews of space missions are exposed to a mixed radiation field, including sparsely and densely ionizing radiation. To determine the biological effectiveness of mixed high-/low-LET radiation fields, mammalian cells were exposed in vitro simultaneously to X-rays and heavy ions, accelerated at the HIMAC accelerator. X-ray doses ranged from 1 to 11 Gy. At the same time, cells were exposed to either 40Ar (550 MeV/n, 86 keV/micrometers), 28Si (100 MeV/n, 150 keV/micrometers), or 56Fe (115 MeV/n, 442 keV/micrometers) ions. Survival was measured in hamster V79 fibroblasts. Structural aberrations in chromosome 2 were measured by chemical-induced premature chromosome condensation combined with fluorescence in situ hybridization in isolated human lymphocytes. For argon and silicon experiments, measured damage in the mixed radiation field was consistent with the value expected using an additive function for low- and high-LET separated data. A small deviation from a simple additive function is observed with very high-LET iron ions combined to X-rays.     

Physical events in the track structure of heavy ions and their relation to alterations of biomolecules.

Heavy charged particles interacting with biological cells can produce a wide variety of different physical, chemical and biological consequences. A rigorous identification of relevant chemical and biological alterations of biomolecules in cells, however, is still lacking and, thus, it is difficult to identify the potential biological importance of different early physical events. In addition, due to experimental and theoretical problems also little is known about the details of energy transfer, -absorption and -decay from projectiles to atoms/molecules in condensed targets; this is particularly true for not completely stripped heavy ions. Nevertheless, one might conclude from available data that higher densities of physical energy absorption events have a significantly higher probability to lead to qualitatively more severe biochemical alterations as regards the induction of DNA double strand breaks and of chromatin damage. It is not very likely that energy migration along the DNA molecule in biological cells over long distances plays a significant role as contributor to these biological radiation effects.     

Effects of heavy ions on visual function and electrophysiology of rodents: the ALTEA-MICE project.

ALTEA-MICE will supplement the ALTEA project on astronauts and provide information on the functional visual impairment possibly induced by heavy ions during prolonged operations in microgravity. Goals of ALTEA-MICE are: (1) to investigate the effects of heavy ions on the visual system of normal and mutant mice with retinal defects; (2) to define reliable experimental conditions for space research; and (3) to develop animal models to study the physiological consequences of space travels on humans. Remotely controlled mouse setup, applied electrophysiological recording methods, remote particle monitoring, and experimental procedures were developed and tested. The project has proved feasible under laboratory-controlled conditions comparable in important aspects to those of astronauts' exposure to particle in space. Experiments are performed at the Brookhaven National Laboratories [BNL] (Upton, NY, USA) and the Gesellschaft für Schwerionenforschung mbH [GSI]/Biophysik (Darmstadt, FRG) to identify possible electrophysiological changes and/or activation of protective mechanisms in response to pulsed radiation. Offline data analyses are in progress and observations are still anecdotal. Electrophysiological changes after pulsed radiation are within the limits of spontaneous variability under anesthesia, with only indirect evidence of possible retinal/cortical responses. Immunostaining showed changes (e.g. increased expression of FGF2 protein in the outer nuclear layer) suggesting a retinal stress reaction to high-energy particles of potential relevance in space.     

Direct measurement of the composition and spectra of cosmic rays above 1 TeV/amu from Jacee

Direct measurements on cosmic ray protons through iron above about 1 TeV/amu have been performed in a series of balloon-borne experiments with emulsion chambers. The measured energy spectra of protons and helium are power laws with exponents of 2.77 ± 0.09 and 2.72 ± 0.11 in the energy range 5 to 500 TeV and 2 to 50 TeV/amu, respectively. The proton spectrum shows no evidence of the steepening near 2 TeV which was reported by other experiments. Helium has a slightly higher intensity compared to extrapolations from lower energy measurements. The heavier elements, carbon to sulfur, show a small tendency for intensity enhancement in the relative abundance above 10 TeV/amu.     

Cellular and subcellular effect of heavy ions: a comparison of the induction of strand breaks and chromosomal aberration with the incidence of inactivation and mutation.

Radiobiological effects of heavy charged particles are compared for a large variety of ions from Helium to Uranium and energies between 1 and 1000 MeV/u which correspond to LET values between 10 and 16000 keV/micrometers. The different cross section for the induction of strand breaks and chromosomal aberrations as well as for inactivation and mutation induction exhibit striking similarities when compared as function of the linear energy transfer (LET). At LET values below 100 keV/micrometers all data points of one specific effect form one single curve as a function of LET, independent of the atomic number of the ion. In this LET range, the biological effects are independ from the particle energy or track structure and depend only on the energy transfer. Therefore, LET is a good parameter in this regime. For LET values greater than 100 keV/micrometers, the curves for the different ions separate from the common curve in order of increasing atomic numbers. In this regime LET is no longer a good parameter and the physical parameters of the formation of particle tracks are important. The similarity of the sigma-LET curves for different endpoints indicates that the 'hook-structure' is produced by physical and chemical effects which occur before the biologically relevant lesions are formed. However, from the existing data of biological effects, it can be concluded that the efficiencies for cell killing are always smaller than those extrapolated from X-ray data on the basis of the energy deposition only. Therefore, cells which are directly hit by an HZE particle are not killed and undergo a finite risk of mutation and transformation.     

Gravitropism and phototropism in protonemata of the moss Pohlia nutans (hedw.) Lindb

The gravitropism of protonemata of Pohlia nutans is described and compared with that of other mosses. In darkness, protonemata showed negative gravitropism. Under uniform illumination they grew radially over the substrate surface, whereas unilateral illumination induced positive phototropic growth. Gravitropism was coupled with starch synthesis and amyloplast formation. Protonematal gravitropic growth is more variable than the strict negative gravitropism of Ceratodon chloronema.     

Studies of the radiation budget anomalies over Antarctica during 1974–1983, and their possible relationship to climatic variations

We present the results of a study of anomalies, which are defined as differences of seasonal means from the data set seasonal means, in the Earth's radiation budget from the analysis of nine years of ten day mean observations derived from the NOAA polar orbiter satellites for the period, 1974–1983. We estimate that the standard deviation in the outgoing longwave flux for this period is less than 12 Wm^?2 and typically 7 Wm^?2. The results show that there are several geographical areas for which the standard deviation is in excess of 20 Wm^?2; in such regions the radiation budget anomalies exceeded these due to natural atmospheric variability. In this paper we discuss the relationship of these anomalies with climatic change.     

Optical and electron-microscopic studies of the Funaria hygrometrica protonema after cultivation for 96 days in space

$\text{Funaria hygrometrica}$ protonema cells grown in the “IFS-2” (Inoculating fixing system) for 96 days on board the Salyut 6 — Soyuz 32 orbital scientific station were examined by light and electron-microscopy. Investigation of experimental and control cells of the moss protonema showed common features as well as distinctions in their structure. Protonema cells of $\text{Funaria hygrometrica}$ both differentiate and undergo photosynthesis during space flight. Changes in cell shape, decreased cell size, a reduction in the volume of starch granules, and altered chloroplast structure were observed.     

Survey of the vestibulum, and behavior of xenopus laevis larvae developed during a 7-days space flight

Aquatic animals have almost no body weight related proprioception for spatial orientation. larvae, like fish, maintain their attitude in water by continuous correction with their fin(s). For these reasons a special performance of the equilibrium system compared to terrestrial animals is necessary. Evidently fish therefore have more compact (dense) otoliths; larvae have less dense otolith (membranes) similar to land vertebrates; but their sacculus-otoliths are vertically positioned, which also may lead to a higher g-sensitivity.

For plausibility reasons gravity should influence the embryonic development of gravity receptors. Yet, evaluations of photographs taken from the surface of cut deep-frozen objects by incident light show no aberration of the shape of the whole vestibulum and of the shape, density, size and position of the otolith membrane in larvae developed under near-zero g (NEXPA-BW-STATEX in D1-Mission).

The further evaluation of the “weightless-larvae” revealed a probably not yet described statolith-like formation in the dorsal wall of the vestibulum. In the weightless larvae this formation outnumbers, also qualitatively, strongly the 1-g controls.

An extra result is the lack of striking effects of cosmic radiation on the embryonic development of the flown eggs.

The swimming behavior of the larvae which was observed about one hour after landing of the Space Shuttle showed a typical anomaly (loop swimming), which is known from larvae developed on the clinostat or from fish flown aboard Apollo capsules.     

The role of drift and convection–diffusion mechanisms in small energy global cosmic ray modulation: Application to the hysteresis phenomenon of proton and alpha particle satellite data

The hysteresis effect for small energies of galactic cosmic rays is due to two effects. The first is the same as for neutron monitor energies – the delay of the interplanetary processes responsible for cosmic ray modulation with respect to the initiating solar processes, according to the effective velocity of solar wind and shock waves propagation. Then, the observed cosmic ray intensity is connected to the solar activity variations during many months before the time of cosmic ray measurement. The second is caused by the time delay of small energy cosmic ray diffusion from the boundary of modulation region to the Earth’s orbit. The model describing the connection between solar activity variation and cosmic ray convection–diffusion global modulation for neutron monitor energies is here developed by taking into account also the time-lag of the small energy particle diffusion in the Heliosphere. We use theoretical results on drifts and analytically approximate the dependences of drifts from tilt angle, and take into account the dependence from the sign of primary particles, and from the sign of polar magnetic field (A > 0 or A < 0). The obtained results are applied on proton and alpha-particle satellite data. We analyze satellite 5-min data of proton fluxes with energies >1 MeV, >2 MeV, >5 MeV, >10 MeV, >30 MeV, >50 MeV, >60 MeV, >100 MeV, and in intervals 10–30 MeV, 30–60 MeV, and 60–100 MeV during January 1986–December 1999. We exclude periods with great cosmic ray increases caused by particle acceleration in solar flare events. Then, we determine monthly averaged fluxes, as well as 5-month and 11-month smoothed data. We analyze also satellite 5-min data on alpha-particle fluxes in the energy intervals 60-160 MeV, 160–260 MeV and 330–500 MeV during January 1986–May 2000. We correct observation data for drifts and then compare with what is expected according to the convection–diffusion mechanism. We assume different dimensions of the modulation region (by the time propagation X₀ of solar wind from the Sun to the boundary of modulation region), for X₀ values from 1 to 60 average months, by one-month steps. For each value of X₀ we determine the correlation coefficient between variations of expected and observed cosmic ray intensities (the estimation of cosmic ray intensities values is given in Section 3 by Eq. (9), and the determination of correlation and regression coefficients in Section 3 by Eq. (8)). The dimension of modulation region is determined by the value of X_0 max, for which the correlation coefficient reaches the maximum value. Then the effective radial diffusion coefficient and residual modulation in small energy region can be estimated.     

Heterogeneous solid/gas chemistry of organic compounds related to comets,meteorites, Titan,and Mars: Laboratory and in lower Earth orbit experiments

To understand the evolution of organic molecules involved in extraterrestrial environments and with exobiological implications, many experimental programs in the laboratory are devoted to photochemical studies in the gaseous phase as well as in the solid state. The validity of such studies and their applications to extraterrestrial environments can be questioned as long as experiments conducted in space conditions, with the full solar spectrum, especially in the short wavelength domain, have not been implemented. The experiments that are described here will be carried out on a FOTON capsule, using the BIOPAN facility, and on the International Space Station, using the EXPOSE facility. Vented and sealed exposition cells will be used, which will allow us to study the chemical evolution in the gaseous phase as well as heterogeneous processes, such as the degradation of solid compounds and the release of gaseous fragments.     

Response of dissolved oxygen and related marine ecological parameters to a tropical cyclone in the South China Sea

It is well known that tropical cyclones can cause upwelling, decrease of sea surface temperature, increase of chlorophyll-a (Chl-a) concentration and enhancement of primary production. But little is known about the response of dissolved oxygen (DO) concentration to a typhoon in the open ocean. This paper investigates the impact of a typhoon on DO concentration and related ecological parameters using in situ and remote sensing data. The in situ data were collected 1 week after the passage of the super-typhoon Nanmadol in the northern South China Sea in 2011. An increase in DO concentration, accompanied by a decrease in water temperature and an increase in salinity and Chl-a concentration, was measured at sampling stations close to the typhoon track. At these stations, maximum DO concentration was found at a depth of around 5 m and maximum Chl-a concentration at depths between 50 and 75 m. The layer of high DO concentration extends from the surface to a depth of 35 m and the concentrations stay almost constant down to this depth. Due to the passage of the typhoon, also a large sea level anomaly (21.6 cm) and a high value of Ekman pumping velocity (4.0 × 10⁻⁴ m s⁻¹) are observed, indicating upwelling phenomenon. At the same time, also intrusion of Kuroshio waters in the form of a loop current into the South China Sea (SCS) was observed. We attribute the increase of DO concentration after the passage of the typhoon to three effects: (1) entrainment of oxygen from the air into the upper water layer and strong vertical mixing of the water body due to the typhoon winds, (2) upwelling of cold nutrient-rich water which stimulates photosynthesis of phytoplankton and thus the generation of oxygen, which also increases the DO concentration due to cold water since the solubility of oxygen increase with decreasing water temperature, and, possibly, (3) transport of DO enriched waters from the Western Pacific to the SCS via the intrusion of Kuroshio waters.     

Tolerance of chufa (Cyperus esculentus L.) plants,representing the higher plant compartment in bioregenerative life support systems,to super-optimal air temperatures

Plants intended to be included in the photosynthesizing compartment of the bioregenerative life support system (BLSS) need to be studied in terms of both their production parameters under optimal conditions and their tolerance to stress factors that might be caused by emergency situations. The purpose of this study was to investigate tolerance of chufa (Cyperus esculentus L.) plants to the super-optimal air temperature of 45 ± 1 °C as dependent upon PAR (photosynthetically active radiation) intensity and the duration of the exposure to the stress factor. Chufa plants were grown hydroponically, on expanded clay, under artificial light. The nutrient solution was Knop’s mineral medium. Until the plants were 30 days old, they had been grown at 690 μmol m⁻² s⁻¹ PAR and air temperature 25 °C. Thirty-day-old plants were exposed to the temperature 45 °C for 6 h, 20 h, and 44 h at PAR intensities 690 μmol m⁻² s⁻¹ and 1150 μmol m⁻² s⁻¹. The exposure to the damaging air temperature for 44 h at 690 μmol m⁻² s⁻¹ PAR caused irreversible damage to PSA, resulting in leaf mortality. In chufa plants exposed to heat shock treatment at 690 μmol m⁻² s⁻¹ PAR for 6 h and 20 h, respiration exceeded photosynthesis, and CO₂ release in the light was recorded. Functional activity of photosynthetic apparatus, estimated from parameters of pulse-modulated chlorophyll fluorescence in Photosystem 2 (PS 2), decreased 40% to 50%. After the exposure to the stress factor was finished, functional activity of PSA recovered its initial values, and apparent photosynthesis (P_apparent) rate after a 20-h exposure to the stress factor was 2.6 times lower than before the elevation of the temperature. During the first hours of plant exposure to the temperature 45 °C at 1150 μmol m⁻² s⁻¹ PAR, respiration rate was higher than photosynthesis rate, but after 3–4 h of the exposure, photosynthetic processes exceeded oxidative ones and CO₂ absorption in the light was recorded. At the end of the 6-h exposure, P_apparent rate was close to that recorded prior to the exposure, and no significant changes were observed in the functional activity of PSA. At the end of the 20-h exposure, P_apparent rate was close to its initial value, but certain parameters of the functional activity of PSA decreased 25% vs. their initial values. During the repair period, the parameters of external gas exchange recovered their initial values, and parameters of pulse-modulated chlorophyll fluorescence were 20–30% higher than their initial values. Thus, exposure of chufa plants to the damaging temperature of the air for 20 h did not cause any irreversible damage to the photosynthetic apparatus of plants at either 690 μmol m⁻² s⁻¹ or 1150 μmol m⁻² s⁻¹ PAR, and higher PAR intensity during the heat shock treatment enhanced heat tolerance of the plants.     

Effects of 40Ar and 56Fe ions on retinal photoreceptor cells of the rabbit: implications for manned missions to Mars.

Losses of photoreceptor cells (rods) from the retinas of New Zealand white (NZW) rabbits were detectable within 2 years after localized acute irradiation of optic and proximal tissues with > or = 7 Gy of 530 MeV u-1 40Ar ions or > or = 2 Gy of 465 MeV u-1 56Fe ions in the Bragg plateau region of energy deposition. Those limits were determined only from an analysis of variance of dose groups because the shapes of the dose response curves at early post-irradiation times are not known, a concern being addressed by experiments in progress. Losses of photoreceptor cells for the period 0.5-2.5 years post-irradiation, determined by provisional linear regression analysis, were approximately 1.7% Gy-1 and 2.5% Gy-1 for 40Ar and 56Fe ions, respectively.     

Present results of the joint radiobiological ESA/IBMP experiments “seeds” aboard Cosmos 2044 and 2229 - correlation of micro-dosimetric data and damage endpoints in Arabidopsis thaliana plants

Experimental data obtained from two Cosmos missions (2044, 2229) performed with dry plant seeds of Arabidopsis thaliana in a Biostack configuration are compared. Biological stress and genetic risk are induced in dry seeds by cosmic radiation, especially the high energetic, densely ionizing component of eavy ons (HI). Subdivision of impacting HI particles into LET-groups (15–35; 35–100; >100 keV/μm) showed the contribution of each LET group to the induction of different biological damage endpoints (survival, cell transformation, lethal plant development). An attempt is presented to comprehend the influence of the spatial energy deposition as a biophysical HI track parameter.     

The role of the society of Latin American specialists on remote sensing (SELPER) in the analysis and actions related to the main advances and needs of spatial remote sensing for Latin America

The existence of SELPER (Sociedad de Especialistas Latinoamericanos en Percepción Remota / Society of Latinamerican Specialists on Remote Sensing) has filled a great gap among latinamerican countries. SELPER was formed in 1980 and several important activities, having international support, have been performed and are planned in the near future. SELPER consolidation will help develop several important regional cooperation programs and the next years look very promisory in this sense. Different steps are planned but the most important is related with the formation of such a Latin American Council on Remote Sensing, having official support from different countries of the region; SELPER can help this important objective. Main advances and needs are summarized in this paper and it is possible to conclude that SELPER will be important for regional and inter-regional scientific and technical cooperation on remote sensing.