Photosynthesis and respiration of a wheat stand at reduced atmospheric pressure and reduced oxygen.

A 34-day functional test was conducted in Johnson Space Center's Variable Pressure Growth Chamber (VPGC) to determine responses of a wheat stand to reduced pressure (70 kPa) and modified partial pressures of carbon dioxide and oxygen. Reduced pressure episodes were generally six to seven hours in duration, were conducted at reduced ppO2 (14.7 kPa), and were interrupted with longer durations of ambient pressure (101 kPa). Daily measurements of stand net photosynthesis (Pn) and dark respiration (DR) were made at both pressures using a ppCO2 of 121 Pa. Corrections derived from leakage tests were applied to reduced pressure measurements. Rates of Pn at reduced pressure averaged over the complete test were 14.6% higher than at ambient pressure, but rates of DR were unaffected. Further reductions in ppO2 were achieved with a molecular sieve and were used to determine if Pn was enhanced by lowered O2 or by lowered pressure. Decreased ppO2 resulted in enhanced rates of Pn, regardless of pressure, but the actual response was dependent on the ratio of ppO2/ppCO2. Over the range of ppO2/ppCO2 of 80 to 200, the rate of Pn declined linearly. Rate of DR was unaffected over the same range and by dissolved O2 levels down to 3.1 ppm, suggesting that normal rhizosphere and canopy respiration occur at atmospheric ppO2 levels as low as 11 kPa. Partial separation of effects attributable to oxygen and those related to reduced pressure (e.g. enhanced diffusion of CO2) was achieved from analysis of a CO2 drawdown experiment. Results will be used for design and implementation of studies involving complete crop growth tests at reduced pressure.     

Effects of long-term low atmospheric pressure on gas exchange and growth of lettuce

The objectives of this research were to determine photosynthesis, evapotranspiration and growth of lettuce at long-term low atmospheric pressure. Lettuce (Lactuca sativa L. cv. Youmaicai) plants were grown at 40 kPa total pressure (8.4 kPa _pO₂$p_{{\text{O}}_2}$) or 101 kPa total pressure (20.9 kPa _pO₂$p_{{\text{O}}_2}$) from seed to harvest for 35 days. Germination rate of lettuce seeds decreased by 7.6% at low pressure, although this was not significant. There was no significant difference in crop photosynthetic rate between hypobaria and ambient pressure during the 35-day study. The crop evapotranspiration rate was significantly lower at low pressure than that at ambient pressure from 20 to 30 days after planting (DAP), but it had no significant difference before 20 DAP or after 30 DAP. The growth cycle of lettuce plants at low pressure was delayed. At low pressure, lettuce leaves were curly at the seedling stage and this disappeared gradually as the plants grew. Ambient lettuce plants were yellow and had an epinastic growth at harvest. The shoot height, leaf number, leaf length and shoot/root ratio were lower at low pressure than those at ambient pressure, while leaf area and root growth increased. Total biomass of lettuce plants grown at two pressures had no significant difference. Ethylene production at low pressure decreased significantly by 38.8% compared with ambient pressure. There was no significant difference in microelements, nutritional phytochemicals and nitrate concentrations at the two treatments. This research shows that lettuce can be grown at long-term low pressure (40 kPa) without significant adverse effects on seed germination, gas exchange and plant growth. Furthermore, ethylene release was reduced in hypobaria.     

Sterilisation properties of the Mars surface and atmospheric environment.

The radiative and chemical conditions at the surface and in the lower Martian atmosphere are computed at various latitudes and seasons combining a 2D photochemical model and radiation simulations. In most situations, the solar UV B and C radiations reach the surface however, suspended dust and, in polar cases, ozone can constitute an effective UV shield. The daytime and night time concentrations of the sterilizing oxidants: OH, H2O2 and O3 are determined, as well as the concentration of the substances which could influence the metabolism of microorganisms. The possible habitats of a remaining Mar's life as well as the possibilities of contamination by resistant earth life forms will be described.     

The motion of an artificial satellite under the terrestrial radiation pressure

The terrestrial infrared radiation pressure effects on the motion of an artificial satellite are investigated. The radiative field is described by a series of spherical harmonics. The equations for the changes of the elements are found and numerical examples for the case of the satellite D-5-B (1975 39 B) are given.     

Growth protocols for etiolated soybeans germinated within BRIC-60 canisters under spaceflight conditions.

As part of the GENEX (Gene Expression) spaceflight experiment, protocols were developed to optimize the inflight germination and subsequent growth of 192 soybean (Glycine max cv McCall) seeds during STS-87. We describe a method which provided uniform growth and development of etiolated seedlings while eliminating root and shoot restrictions for short-term (4-7 day) experiments. Final seedling growth morphologies and the gaseous CO2 and ethylene levels present both on the last day in space and at the time of recovery within the spaceflight and ground control BRIC-60 canisters are presented.     

Vegetation and the atmospheric cycling of mercury

Beyond their role in the cycling of the major elements, carbon, nitrogen, oxygen, etc., little is known about geobiological interactions involving plants with the elements of lower abundance. Plants influence the distribution of volcanic mercury by uptake from the atmosphere and soil fluids, transport to the shoot and re-release as Hg° into the atmosphere. Release rates as high as 2.5 × 10^?4 g.h^?1 per kg are known but even at more typical rates, fluxes far greater than the Environmental Protection Agency calculated U.S. average annual degassing rate of 130 × 10^?6 g.m^?2 should exist. On a global basis, the presence or absence of vegetative cover may be a significant regulator of Hg° transport between land surfaces and the atmosphere.     

Growth and development in higher plants under simulated microgravity conditions on a 3-dimensional clinostat.

Growth and development of etiolated pea (Pisum sativum L. cv. Alaska) and maize (Zea mays L. cv. Golden Cross Bantam) seedlings grown under simulated microgravity conditions were intensively studied using a 3-dimensional clinostat as a simulator of weightlessness. Epicotyls of etiolated pea seedlings grown on the clinostat were the most oriented toward the direction far from cotyledons. Mesocotyls of etiolated maize seedlings grew at random and coleoptiles curved slightly during clinostat rotation. Clinostat rotation promoted the emergence of the 3rd internodes in etiolated pea seedlings, while it significantly inhibited the growth of the 1st internodes. In maize seedlings, the growth of coleoptiles was little affected by clinostat rotation, but that of mesocotyls was suppressed, and therefore, the emergence of the leaf out of coleoptile was promoted. Clinostat rotation reduced the osmotic concentration in the 1st internodes of pea seedlings, although it has little effect on the 2nd and the 3rd internodes. Clinostat rotation also reduced the osmotic concentrations in both coleoptiles and mesocotyls of maize seedlings. Cell-wall extensibilities of the 1st and the 3rd internodes of pea seedlings grown on the clinostat were significantly lower and higher as compared with those on 1 g conditions, respectively. Cell-wall extensibility of mesocotyls in seedlings grown on the clinostat also decreased. Changes in cell wall properties seem to be well correlated to the growth of each organ in pea and maize seedlings. These results suggest that the growth and development of plants is controlled under gravity on earth, and that the growth responses of higher plants to microgravity conditions are regulated by both cell-wall mechanical properties and osmotic properties of stem cells.     

Yield comparisons and unique characteristics of the dwarf wheat cultivar 'USU-Apogee'.

Extremely short, high yielding cultivars of all crop plants are needed to optimize the food production of bioregenerative life support systems in space. In the early 1980's, we examined over a thousand wheat genotypes from the world germplasm collection in search of genotypes with appropriate characteristics for food production in space. Here we report the results of 12 years of hybridization and selection for the perfect wheat cultivar. 'USU-Apogee' is a full-dwarf hard red spring wheat (Triticum aestivum L.) cultivar developed for high yields in controlled environments. USU-Apogee was developed by the Utah Agricultural Experiment Station in cooperation with the National Aeronautics and Space Administration and released in April 1996. USU-Apogee is a shorter, higher yielding alternative to 'Yecora Rojo' and 'Veery-10', the short field genotypes previously selected for use in controlled environments. The yield advantage of USU-Apogee is 10 to 30% over these other cultivars, depending on environmental conditions. USU-Apogee (45-50 cm tall, depending on temperature) is 10 to 15 cm shorter than Yecora Rojo and 1 to 4 cm shorter than Veery-10. USU-Apogee was also selected for resistance to the calcium-induced leaf tip chlorosis that occurs in controlled-environments. Breeder seed of USU-Apogee will be maintained by the Crop Physiology Laboratory and seed is available for testing on request.     

Growth of Prunus tree stems under simulated microgravity conditions

Stem growth of Prunus trees under simulated microgravity conditions was examined using a three-dimensional clinostat. The stems elongated with bending under such conditions. Stem elongation and leaf expansion were both promoted, whereas the formation of xylem in the secondary thickening growth was inhibited under the simulated microgravity condition. In secondary xylem, sedimentable amyloplasts were observed in the 1g control. The present results suggest that stem elongation and leaf expansion may be inhibited at 1g, while growth direction and secondary xylem formation depend on a gravity stimulus. A space experiment is expected to advance research on thickening growth in trees.     

Overview of atmospheric ionizing radiation (AIR) research: SST-present.

The Supersonic Transport (SST) program, proposed in 1961, first raised concern for the exposure of pregnant occupants by solar energetic particles (SEP), and neutrons were suspected to have a main role in particle propagation deep into the atmosphere. An eight-year flight program confirmed the role of SEP as a significant hazard and of the neutrons as contributing over half of the galactic cosmic ray exposures, with the largest contribution from neutrons above 10 MeV. The FAA Advisory Committee on the Radiobiological Aspects of the SST provided operational requirements. The more recent lowering of ICRP-recommended exposure limits (1990) with the classification of aircrew as "radiation workers" renewed interest in GCR background exposures at commercial flight altitudes and stimulated epidemiological studies in Europe, Japan, Canada and the USA. The proposed development of a High Speed Civil Transport (HSCT) required validation of the role of high-energy neutrons, and this resulted in ER-2 flights at solar minimum (June 1997) and studies on effects of aircraft materials on interior exposures. Recent evaluation of health outcomes of DOE nuclear workers resulted in legislation for health compensation in year 2000 and recent European aircrew epidemiological studies of health outcomes bring renewed interest in aircraft radiation exposures. As improved radiation models become available, it is imperative that a corresponding epidemiological program of US aircrew be implemented.     

The importance of restoration of the atmospheric electrical environment in closed Bioregenerative Life Supporting Systems.

Earth based Bioregenerative Life Supporting Systems (BLSS) are subject to 4 main physical factors: gravity, light, temperature and electrical environment. The first 3 are obvious to everyone, the Electrical Environment (EE) is not under the majority of prevailing conditions perceived directly by our senses. The EE is one of the important physical factors directly influencing some plants and in a less obvious way also the majority of plants. There are only two long range forces in nature: the electromagnetic and the gravitational forces. Gravity is very much weaker than the electromagnetic forces FG/FEL=10(-38), where FG is the gravitational Force and FEL are the electromagnetic Forces. The atmospheric electric field prevails all the time over the entire Earth with a mean intensity of 130 V/m. It is therefore a potent factor which may be used by some plants exposed throughout their entire life time to the atmospheric electric field. What effect should the normal atmospheric electric field have on plants? All living plants are good electrical conductors for electrostatic fields. The plants distort the normally vertical field lines, which have to be perpendicular to the plant tissue everywhere in order to avoid the extraction of energy from the field. The meristems concentrate the field lines, thus the electrically charged nutrients are supplied to the growing parts of the plant exposed to the field. This results in electrotropism in some plants. It is very well known that plants do have adaptive capabilities as compared to animals, it is important for their survival, because they cannot run away from trouble. It is found by careful observations of the behaviour of different plants that some plants do respond to the presence of the atmospheric electric field while other plants exposed to the same environment are indifferent to the atmospheric electric field. The plants growing in the Biosphere II were shielded by the metal structure of the Biosphere II. Because these plants which do make use of the atmospheric electric field are also to be found in the Biosphere II and must be deprived of this natural atmospheric electric field and in consequence of some of their natural nutrients. I have experimental evidence that at least some plants do respond to the atmospheric electric field. This effect is the most likely cause of the oxygen depletion and of the carbon dioxide accumulation in Biosphere II. Under the micro gravity encountered in space habitats the restoration of electric fields is even more important for plant growth than it is on the Earth.     

Thymine photoproduct formation and inactivation of intact spores of Bacillus subtilis irradiated with short wavelength UV (200-300nm) at atmospheric pressure and in vacuo.

Vacuum exposure renders the survival of spores of Bacillus subtilis approximately five times more sensitive to ultraviolet light irradiation than exposure under atmospheric conditions. The photoproduct formation in spores irradiated under ultrahigh vacuum (UHV) conditions is compared to the photoproduct formation in spores irradiated at atmospheric pressure. Compared to irradiation at atmospheric pressure, where only the "spore photoproduct" 5-thyminyl-5,6-dihydrothymine (TDHT) can be detected, two additional photoproducts, known as the c,s and t,s isomers of thymine dimer (T<>T) are produced in vacuo. The spectral efficiencies for photoproduct formation in spores under atmospheric and vacuum conditions are compared. Since there is no increased formation of TDHT after irradiation in vacuum, TDHT cannot be made responsible for the observed vacuum effect. "Vacuum specific" photoproducts may cause a synergistic response of spores to the simultaneous action of ultraviolet light (UV) and UHV. Three different mechanisms are discussed for the enhanced sensitivity of B. subtilis spores to UV radiation in vacuum. The experiments described contribute valuable research information on the chance for survival of microorganisms in outer space.     

The influence of microbial associations on germination of wheat seeds and growth of seedlings under impact of zinc salts

The life support systems (LSS) for long-term missions are to use cycling-recycling systems, including biological recycling. Higher plants are the traditional regenerator of air and producer of food. They should be used in many successive generations of their reproduction in LSS.     

A simplified closed artificial ecosystem--recycling of organic matter into wheat plants.

A simplified closed system consisting of a plant growth chamber coupled to a decomposition chamber was used to study carbon exchange dynamics. The CO2 produced via the decomposition of wheat straw was used for photosynthetic carbon uptake by wheat plants. The atmosphere of the two chambers was connected through a circuit of known flow rate. Thus, monitoring the CO2 concentrations in both compartments allowed measurement of the carbon exchange between the chambers, and estimation of the rate of respiration processes in the decomposition chamber and photosynthetic rate in the producer chamber. The objective for CELSS research was to simulate a system where a compartment producing food via photosynthesis, would be supplied by CO2 produced from respiration processes. The decomposition of biomass by the decomposer simulated both the metabolism of a crew and the result of a recycling system for inedible biomass. Concerning terrestrial ecosystems, the objective was to study organic matter decomposition in soil and other processes related to permanent grasslands.     

Increase of atmospheric CO2: response patterns of a simple terrestrial man-made ecosystem.

Simple models of terrestrial ecosystems with a limited number of components are an efficient tool to study the main laws of functioning of populations, including microbial ones, and their communities, as components of natural ecosystems, under variable environmental conditions. Among other factors are the increase of carbon dioxide in the atmosphere and limitation of plants' growth by biogenic elements. The main types of ecosystems' responses to changes in environmental conditions (a change in CO2 concentration) have been demonstrated in a "plants-rhizospheric microorganisms-artificial soil" simple experimental system. The mathematical model of interactions between plants and microorganisms under normal and elevated atmospheric CO2 and limitation by nutrients (nitrogen and phosphorus) yielded a qualitative agreement between calculated and experimental values of limiting substances concentrations and release rates of exudates.     

Atmospheric dynamics in the "Laboratory Biosphere" with wheat and sweet potato crops.

Laboratory Biosphere is a 40-m3 closed life system equipped with 12,000 W of high pressure sodium lamps over planting beds with 5.37 m2 of soil. Atmospheric composition changes due to photosynthetic fixation of carbon dioxide and corresponding production of oxygen or the reverse, respiration, are observed in short timeframes, e.g., hourly. To focus on inherent characteristics of the crop as distinct from its area or the volume of the chamber, we report fixation and respiration rates in mmol h-1 m-2 of planted area. An 85-day crop of USU Apogee wheat under a 16-h lighted/8-h dark regime peaked in fixation rate at about 100 mmol h-1 m-2 approximately 24 days after planting. Light intensity was about 840 micromoles m-2 s-1. Dark respiration peaked at about 31 mmol h-1 m-2 at the same time. Thereafter, both fixation and respiration declined toward zero as harvest time approached. A residual soil respiration rate of about 1.9 mmol h-1 m-2 was observed in the dark closed chamber for 100 days after the harvest. A 126-day crop of Tuskegee TU-82-155 sweet potato behaved quite differently. Under a 680 micromoles m-2 s-1, 18-h lighted/6-h dark regime, fixation during lighted hours rose to a plateau ranging from about 27 to 48 mmol h-1 m-2 after 42 days and dark respiration settled into a range of 12-23 mmol h-1 m-2. These rates continued unabated until the harvest at 126 days, suggesting that tuber biomass production might have continued at about the same rate for some time beyond the harvest time that was exercised in this experiment. In both experiments CO2 levels were allowed to range widely from a few hundred to about 3000 ppm, which permitted observation of fixation rates both at varying CO2 concentrations and at each number of days after planting. This enables plotting the fixation rate as a function of both variables. Understanding the atmospheric dynamics of individual crops will be essential for design and atmospheric management of more complex CELSS which integrate the simultaneous growth of several crops as in a sustainable remote life support system.     

Some properties of the polar energy source and of the associated atmospheric perturbations

The magnitude, dissipation mechanism, and spatial distribution of the solar wind - magnetospheric energy source are discussed briefly. Using N₂ measurements of the ESRO 4 satellite, the temperature increase in the polar thermosphere associated with this energy source are investigated. Part of the locally dissipated energy is transported toward lower latitudes. Possible modes of energy transfer are reviewed, and local time variations are documented. Some suggestions are made with respect to future empirical models of the thermosphere.     

Remote sensing of the atmospheric from scientific stations “Salyut”

A review of the four-channel teleradiometer “Micron” calibration and data processing techniques has been presented. The sensor validation of the space-borne teleradiometer “Micron” was based on the following: the preflight absolute calibration with the state certificated standard, the secondary onboard standard (miniature filament lamp), the inflight measurements of the Moon's brightness. The procedure of the extinction coefficient determination from the daytime horizon profiles has been developed. The validity of the used techniques was estimated by carring out model calculations. An optimal measuring procedure has been recommended. The proposed simple techniques are used for processing the space measured horizon brightness data. The results have been compared with the earlier published ones.     

Venus atmospheric circulation: Observations and implications of the thermal structure

Continued analysis of Pioneer Venus imaging and polarimetry data indicates that the average cloud-top level circulation is mainly zonal (east to west) with a small meridional component. Presence of planetary scale waves and a possible sun-related component are evident in the data. If the tracked features refer to the same vertical level, then some variability of the circulation would have to be present to account for the Pioneer and Mariner 10 cloud-tracking results. However, the implied balanced flow from the observed thermal structure analysis strongly suggests that at least some of the variations in these observations is due to apparent cloud-top variations and that the circulation itself is relatively stable.Direct cyclostrophic calculations based on the observed thermal structure of the atmosphere yield a balanced zonal circulation with distinct mid-latitude jets (peak velocities about 110–120 ms^?1) located between 50 and 40 mb in each hemisphere of the planet near 45° latitude. The calculations which extend to about 40 km altitude from 80 km above the surface agree well with the observed entry probe zonal components and indicate breakdown of the balance condition near the upper and lower boundaries at low latitudes.The balanced flow results are consistent with the Mariner 10 and Pioneer cloud tracked estimates of the zonal circulation provided the effective altitude of the tracked features is slightly different at different observation periods. The features in the Pioneer Venus data would then lie on a sloping surface that extends from about 68 km (40 mb) at low latitudes to about 75 km (10 mb) in mid-latitudes. The polarization features would occur on a roughly parallel surface that is 1–2 km above the effective cloud-height surface, and Mariner 10 features would have effective altitudes somewhat lower than the Pioneer ultraviolet features. A slight asymmetry is evident in the balanced zonal circulation arising out of an asymmetry in the thermal field.Finally, the solenoids formed by intersecting isobaric and isosteric (constant specific volume) surfaces deduced from the Pioneer Venus radio occultation data show distinct evidence of a direct meridional circulation that may be important in sustaining the Venus atmospheric circulation.     

Sporadic sodium layers and the average vertical distribution of atmospheric sodium

We have used atmospheric sodium profiles obtained at São José dos Campos over the period 1993–2004 to study the influence of sporadic layers on the average sodium distribution. All the profiles for the time period 19–22 h local standard time were individually classified as either normal or sporadic. Profiles were considered sporadic if they contained sharp increases above the background profile by more than a factor of 2, occurring on the topside of the layer, with a width of less than 5 km. For each night’s data, the averages of the normal and sporadic profiles were stored separately. A comparison between the long-term means of these two average profiles, for those days on which both normal and sporadic profiles were observed, showed no significant difference between them. This indicates that, on average, there is no additional sodium in the sporadic profiles. This analysis suggests that at least part of the topside of the normal sodium layer originates in a source that also gives rise to sporadic layers, which would occur when the source itself becomes layered. The most likely source is sodium-containing ions, which would be layered by the tide and/or gravity wave driven wind-shear mechanism generally accepted as being responsible for some types of ionospheric sporadic-E.