The Protein Crystallization Facility (PCF) for EURECA.

Research on the structure of molecules by X-ray diffraction analysis requires large single crystals. However, the dynamic behavior of proteins caused by their high molecular weight prevents the growth of large single crystals if this process is disturbed by thermal convection. For example, protein single crystals grown under terrestrial (1 g) conditions are limited to dimensions in the order of 0.1 mm, whereas the size of crystals, grown under (quasi) space conditions has been 5 times larger (pilot experiment CRYOSTAT, Spacelab). Under EURECA conditions (e.g. no microgravity disturbances), the result in regularity of crystal growth and size is expected to be much better. In this paper an overview is given of the protein crystallization facility which includes Experiment-, Service- and Secondary Cooling Module, and its interfaces to the EURECA Carrier. At the end, there will be presented a short mission profile concerning cooling-, power- and data exchange requirements.     

The evolution of complex life

In considering the probabilities that intelligent life might exist elsewhere in the Universe, it is important to ask questions about the factors governing the emergence of complex living organisms in the context of evolutionary biology, planetary environments and events in space. Two important problems arise. First, what can be learned about the general laws governing the evolution of complex life anywhere in space by studying its history on the Earth? Second, how is the evolution of complex life affected by events in space? To address these problems, a series of Science Workshops on the Evolution of Complex Life was held at the Ames Research Center. Included in this paper are highlights of those workshops, with particular emphasis on the first question, namely the evolution of complex extraterrestrial life.     

The origin and early evolution of membrane channels

The origin and early evolution of ion channels are considered from the point of view that the transmembrane segments of membrane proteins are structurally quite simple and do not require specific sequences to fold. We argue that the transport of solute species, especially ions, required an early evolution of efficient transport mechanisms, and that the emergence of simple ion channels was protobiologically plausible. We also argue that, despite their simple structure, such channels could possess properties that, at the first sight, appear to require markedly greater complexity. These properties can be subtly modulated by local modifications to the sequence rather than global changes in molecular architecture. In order to address the evolution and development of ion channels, we focus on identifying those protein domains that are commonly associated with ion channel proteins and are conserved throughout the three main domains of life (Eukarya, Bacteria, and Archaea). We discuss the potassium-sodium-calcium superfamily of voltage-gated ion channels, mechanosensitive channels, porins, and ABC-transporters and argue that these families of membrane channels have sufficiently universal architectures that they can readily adapt to the diverse functional demands arising during evolution.     

Secular evolution of rotary motion of a charged satellite in a decaying orbit

An electrostatically charged Earth satellite whose orbit is decaying due to the Earths oblateness is considered. Secular perturbations of the orbit are taken into account: they are caused by the second zonal harmonic of the geopotential. These perturbations represent deviations of the longitude of the ascending node and perigee argument, the orbit form being invariable and the orbit inclination to the equatorial plane being constant. The attitude rotary motion of the satellite under the action of perturbing moments of the gravitational and Lorentz forces is studied. The magnetic field of the Earth is taken in a quadrupole approximation. The evolution of the satellites rotary motion is investigated on the basis of new differential equations in s-parameters specially constructed for this purpose. Using the method of averaging, basic regularities of the secular evolution of rotary motion of a screened satellite are revealed. It is found that the rotary motion of a charged satellite essentially depends on the quadrupole component of the geomagnetic potential.__________Translated from Kosmicheskie Issledovaniya, Vol. 43, No. 2, 2005, pp. 111–125.Original Russian Text Copyright © 2005 by Tikhonov.     

Analysis of evolution of multi-orbit perturbed trajectories of a spacecraft in a central field of attraction

Properties of differential equations of multi-orbit trajectory motion of a spacecraft are investigated analytically. The spacecraft moves under the action of small perturbations (in particular, low thrust) in the plane of a central Newtonian field of attraction. The conditions are specified for existence of a partial singular aperiodic solution, in the neighborhood of which the behavior of osculating elements changes sharply. In this case, phase variables (the angular position of the pericenter and the true anomaly) are found to undergo the sharpest changes. The exact superposition of solutions is suggested for the equations of motion transformed to the form of a quasi-linear, weakly non-stationary system: a partial singular aperiodic solution and fast solutions oscillating around it. Asymptotic representations are obtained for both components of the superposition. They are fairly exact in the region of smallness of perturbing terms at a long variation of the argument.     

Design of a high performance suspension for lunar rover based on evolution

In this paper, we propose a new suspension for lunar rover called obverse and reverse four-linkage suspension (ORF-L suspension for short). Its two components are designed based on the evolutions of bogie and rocker. Firstly, we analyze the character of bogie and research the approach to improve its performance. Based on that research, an evolved mechanism of bogie is proposed, named obverse four-linkage. It has better capacity than bogie. In addition an evolved mechanism of rocker is also proposed, named reverse four-linkage. The bogie, rocker and their evolved mechanisms can compose four available suspensions including the interested ORF-L suspension. Because ORF-L suspension is composed of two evolved mechanisms, it has the highest performance. In order to check that, the performance comparison between ORF-L suspension and rocker-bogie suspension are carried out based on simulation. Finally, a prototype rover with ORF-L suspension is designed and manufactured. It shows excellent performance as expected.     

ISS crew evolution

A Russian cosmonaut reviews the composition of U.S. and Russian crews and joint activities on International Space Station Expeditions One through Five.     

On the features of long-term evolution of a high-apogee orbit of the SPECTR-R spacecraft

The practical tasks related to qualitative investigation of long-term evolution of high-apogee orbits of artificial Earth satellites (AES), for which the main perturbing factors are gravitational perturbations from the Moon and the Sun, are considered. Attention is given to the problem of the ballistic lifetime of similar orbits, and the issues associated with possibilities of the correction of orbits for ensuring the required duration of their ballistic lifetime are considered. The orbit of the SPECTR-R spacecraft launched in July of 2011 is considered as an example.     

Gedankenspiele zur kosmischen evolution

To day's predominant opinion about the fact of a finitely large, expanding universe is based on observations of a distance dependent red shift in the spectrum of cosmic light sources, as reported about fifty years ago by the American astronomer E. P. Hubble.

Doubts appeared in the meantime about this hypothesis of expansion. Those as well as the prerequisites to alternate models of the universe and its evolution will be treated.     

A study of evolution of spin-up mode of a satellite in the plane of its orbit

We investigate the mode of spinning up a low-orbit satellite in the plane of its orbit. In this mode the satellite rotates around its principal central axis of the minimum moment of inertia which executes small oscillations with respect to the normal to the orbit plane; the angular velocity of the rotation around this axis several times exceeds the mean orbital motion. Gravitational and restoring aerodynamic moments are taken into account in the satellite’s equations of motion. A small parameter characterizing deviation of the satellite from a dynamically symmetric shape is introduced into the equations. A two-dimensional integral surface of the equations of motion, describing quasi-steady-state rotations of the satellite close to cylindrical precession of the corresponding symmetrical satellite in a gravitational field, has been studied by the method of small parameter and numerically. Such quasi-steady-state rotations are suggested to be considered as unperturbed motions of the satellite in the spin-up mode. Investigation of the integral surface is reduced to numerical solution of a periodic boundary value problem of a certain auxiliary system of differential equations and to calculation of quasi-steady-state rotations by the two-cycle method. A possibility is demonstrated to construct quasi-steady rotations by way of minimization of a special quadratic functional.     

From Halley's Comet to solar terrestrial science : The evolution of the Inter-Agency consultative group

The Inter-Agency Consultative Group (IACG) is an organization which seeks to maximize scientific returns from focused areas of space science through international cooperation. In its 11-year history the IACG has experienced both monumental success (with the collaborative exploration of Comet Halley) and, more recently, some serious growing pains in its second phase of operation, which focuses on solar terrestrial science. In this post-Cold War period, with increased interaction between countries offering greater opportunities for cooperation, the lessons to be learned from the IACG's experience will be valuable ones.     

The role of mud volcanoes in the evolution of Hecate Tholus Volcano on the surface of Mars

Hecate Tholus Volcano has undergone numerous changes in its history of evolution. Further, the phenomena occurring on the surface of this volcano endow us with a remarkable perspective of recent martian geological changes. In the vicinity of this volcano cone, most of the lava is covered with a thick layer of loose sediments (probably clay). The presence of such sediments at the base of the volcano cone has led to the formation of several major landslides. Moreover, liquid water flow on the volcano cone has created a myriad of radial channels. The formation of such structures on the cone of a volcano is only plausible as a result of eruption of a mud volcano from its crater. Besides, the constant discharge of mud-like materials as well as hot water from the volcano paves the way for the growth and evolution of hydrothermal organisms.     

Tides and the evolution of planetary habitability

Tides raised on a planet by the gravity of its host star can reduce the planet's orbital semi-major axis and eccentricity. This effect is only relevant for planets orbiting very close to their host stars. The habitable zones of low-mass stars are also close in, and tides can alter the orbits of planets in these locations. We calculate the tidal evolution of hypothetical terrestrial planets around low-mass stars and show that tides can evolve planets past the inner edge of the habitable zone, sometimes in less than 1 billion years. This migration requires large eccentricities (>0.5) and low-mass stars ( less or similar to 0.35 M(circle)). Such migration may have important implications for the evolution of the atmosphere, internal heating, and the Gaia hypothesis. Similarly, a planet that is detected interior to the habitable zone could have been habitable in the past. We consider the past habitability of the recently discovered, approximately 5 M(circle) planet, Gliese 581 c. We find that it could have been habitable for reasonable choices of orbital and physical properties as recently as 2 Gyr ago. However, when constraints derived from the additional companions are included, most parameter choices that indicate past habitability require the two inner planets of the system to have crossed their mutual 3:1 mean motion resonance. As this crossing would likely have resulted in resonance capture, which is not observed, we conclude that Gl 581 c was probably never habitable.     

SETI in the light of cosmic convergent evolution

Theodosius Dobzhansky, one of the founding fathers of the modern evolutionary synthesis, once famously stated that “nothing makes sense in biology except in the light of evolution”. Here it will be argued that nothing in astrobiology makes sense except in the light of “Cosmic Convergent Evolution” (CCE). This view of life contends that natural selection is a universal force of nature that leads to the emergence of similarly adapted life forms in analogous planetary biospheres. Although SETI historically preceded the rise of astrobiology that we have witnessed in the recent decade, one of its main tenets from the beginning was the convergence of life on a cosmic scale toward intelligent behavior and subsequent communication via technological means. The question of cultural convergence in terms of symbolic exchange, language and scientific capabilities between advanced interstellar civilizations has been the subject of ongoing debate. However, at the core of the search for extraterrestrial intelligence lies in essence a biological problem since even post-biological extraterrestrial intelligences must have had an origin based on self-replicating biopolymers. Thus, SETI assumes a propensity of the Universe towards biogenesis in accordance with CCE, a new evolutionary concept which posits the multiple emergence of life across the Cosmos. Consequently, we have to wonder about the biophilic properties the Universe apparently exhibits, as well as to try to find an encompassing theory that is able to explain this “fine-tuning” in naturalistic terms. The aims of this paper are as follows: 1) to emphasize the importance of convergent evolution in astrobiology and ongoing SETI research; 2) to introduce novel and biology-centered cosmological ideas such as the “Selfish Biocosm Hypothesis” and the “Evo Devo Universe” as valuable arguments in theorizing about the origin and nature of extraterrestrial intelligence and 3) to synthesize these findings within an emerging post-biological paradigm on which future SETI efforts may be founded.     

Impacts and evolution: future prospects

The discipline of astrobiology includes the dynamics of biological evolution. One of the major ways that the cosmos influences life is through the catastrophic environmental disruptions caused when comets and asteroids collide with a planet. We now recognize that such impacts have caused mass extinctions and played a major role in determining the evolution of life on Earth. The time-averaged impact flux as a function of projectile energy can be derived from lunar cratering statistics as well as the current population of near Earth asteroids (NEAs). Effects of impacts of various energies can be modeled, using data from historic impacts [such as the Cretaceous-Tertiary (KT) impactor 65 million years ago] and the observed 1994 bombardment of Jupiter by fragments of Comet Shoemaker-Levy 9. It is of particular interest to find from such models that the terrestrial environment is highly vulnerable to perturbation from impacts, so that even such a small event as the KT impact (by a projectile 10-15 km in diameter) can lead to a mass extinction. Similar considerations allow us to model the effects of still smaller (and much more likely) impacts, down to the size of the asteroid that exploded over Tunguska in 1908 (energy approximately 10 megatons). Combining the impact flux with estimates of environmental and ecological effects reveals that the greatest contemporary hazard is associated with impactors near 1 million megatons in energy (approximately 2 km in diameter for an asteroid). The current impact hazard is significant relative to other natural hazards, and arguments can be developed to illuminate a variety of public policy issues. The first priority in any plan for defense against impactors is to survey the population of Earth-crossing NEAs and project their orbits forward in time. This is the purpose of the Spaceguard Survey, which has already found more than half of the NEAs >1 km in diameter. If there is an NEA on a collision course with Earth, it can be discovered and the impact predicted with decades or more of warning. It is then possible to consider how to deflect or disrupt the NEA. Unlike other natural hazards, the impact risk can be largely eliminated, given sufficient advanced knowledge to take action against the threatening projectile.     

Domain evolution and functional diversification of sulfite reductases

Sulfite reductases are key enzymes of assimilatory and dissimilatory sulfur metabolism, which occur in diverse bacterial and archaeal lineages. They share a highly conserved domain "C-X5-C-n-C-X3-C" for binding siroheme and iron-sulfur clusters that facilitate electron transfer to the substrate. For each sulfite reductase cluster, the siroheme-binding domain is positioned slightly differently at the N-terminus of dsrA and dsrB, while in the assimilatory proteins the siroheme domain is located at the C-terminus. Our sequence and phylogenetic analysis of the siroheme-binding domain shows that sulfite reductase sequences diverged from a common ancestor into four separate clusters (aSir, alSir, dsr, and asrC) that are biochemically distinct; each serves a different assimilatory or dissimilatory role in sulfur metabolism. The phylogenetic distribution and functional grouping in sulfite reductase clusters (dsrA and dsrB vs. aSiR, asrC, and alSir) suggest that their functional diversification during evolution may have preceded the bacterial/archaeal divergence.     

Potential evolution of an international moon base programme

The Moon is a major target in expanding human activity in Space. President Bush has called for a Space Exploration Initiative. European participation may depend on achieving an affordable programme and identifying distinct elements for non-U.S. participation. Affordability requires that all participants can influence the “cost to user” of Base operations. If lunar activity is to evolve towards resource exploitation, there will need to be a progressive reduction in operating costs. European interest would prefer participation that allowed longer-term independent interests. The paper addresses how non-U.S. agencies could contribute valuable elements to an International Moon Base while meeting three criteria:

• — Keep a core infrastructure under U.S. control.

• — Avoid a total reliance by the partner on U.S. services.

• — Allow the partner to evolve towards an eventual, semi-autonomous or autonomous capability.

The paper illustrates possible implications of meeting these constraints through “mini infrastructures” combining several elements to form a working architecture. It is concluded that any European participation in an International Moon Base Programme should contain both Space transport and surface elements.     

Pyrite-induced hydrogen peroxide formation as a driving force in the evolution of photosynthetic organisms on an early earth

The remarkable discovery of pyrite-induced hydrogen peroxide (H2O2) provides a key step in the evolution of oxygenic photosynthesis. Here we show that H2O2 can be generated rapidly via a reaction between pyrite and H2O in the absence of dissolved oxygen. The reaction proceeds in the dark, and H2O2 levels increase upon illumination with visible light. Since pyrite was stable in most photic environments prior to the rise of O2 levels, this finding represents an important mechanism for the formation of H2O2 on early Earth.