Effects of solar UV-B radiation on aquatic ecosystems.

Solar UV degrades dissolved organic carbon photolytically so that they can readily be taken up by bacterioplankton. On the other hand solar UV radiation inhibits bacterioplankton activity. Bacterioplankton productivity is far greater than previously thought and is comparable to phytoplankton primary productivity. According to the "microbial loop hypothesis," bacterioplankton is seen in the center of a food web, having a similar function to phytoplankton and protists. The penetration of UV and PAR into the water column can be measured. Marine waters show large temporal and regional differences in their concentrations of dissolved and particulate absorbing substances. A network of dosimeters (ELDONET) has been installed in Europe ranging from Abisko in Northern Sweden to Gran Canaria. Cyanobacteria are capable of fixing atmospheric nitrogen which is then made available to higher plants. The agricultural potential of cyanobacteria has been recognized as a biological fertilizer for wet soils such as in rice paddies. UV-B is known to impair processes such as growth, survival, pigmentation, motility, as well as the enzymes of nitrogen metabolism and CO2 fixation. The marine phytoplankton represents the single most important ecosystem on our planet and produces about the same biomass as all terrestrial ecosystems taken together. It is the base of the aquatic food chain and any changes in the size and composition of phytoplankton communities will directly affect food production for humans from marine sources. Another important role of marine phytoplankton is to serve as a sink for atmospheric carbon dioxide. Recent investigations have shown a large sensitivity of most phytoplankton organisms toward solar short-wavelength ultraviolet radiation (UV-B); even at ambient levels of UV-B radiation many organisms seem to be under UV stress. Because of their requirement for solar energy, the phytoplankton dwell in the top layers of the water column. In this near-surface position phytoplankton will be exposed to solar ultraviolet radiation. This radiation has been shown to affect growth, photosynthesis, nitrogen incorporation and enzyme activity. Other targets of solar UV irradiation are proteins and pigments involved in photosynthesis. Whether or not screening pigments can be induced in phytoplankton to effectively shield the organisms from excessive UV irradiation needs to be determined. Macroalgae show a distinct pattern of vertical distribution in their habitat. They have developed mechanisms to regulate their photosynthetic activity to adapt to the changing light regime and protect themselves from excessive radiation. A broad survey was carried out to understand photosynthesis in aquatic ecosystems and the different adaptation strategies to solar radiation of ecologically important species of green, red and brown algae from the North Sea, Baltic Sea, Mediterranean, Atlantic, polar and tropical oceans. Photoinhibition was quantified by oxygen exchange and by PAM (pulse amplitude modulated) fluorescence measurements based on transient changes of chlorophyll fluorescence.     

Stability of nucleic acid under the effect of UV radiation.

Nucleic acids (combined with protein molecules) are essential constituents of the living systems playing an important role in the early evolution of life as well. A specific feature of these molecules has been found and directly confirmed recently: under the influence of short-wavelength UV radiation bipyrimidine photoproducts (cyclobutane dimers and 6-4 bipyrimidines) are induced and the reversion of them can be provoked by the same photons. However, reversion is preferred by the shorter wavelengths. With increasing ratio of the longer wavelength components of the radiation (using artificial UV sources and solar light on the Earth's surface) the impact of the reversible photoproducts in the harmful biological effect decreases and other photoproducts are dominant. Assuming the photoinduced reactions (dimerisation and reversion) are statistical events, during the irradiation the chance for a number of nucleoprotein molecules to survive the radiation damage can be reality. The theoretical and experimental basis of these assumptions will be discussed in the case of bacteriophage T7 nucleoprotein.     

The total ozone and UV solar radiation over Stara Zagora,Bulgaria

The results from direct ground-based solar UV irradiance measurements and the total ozone content (TOC) over Stara Zagora (42° 25′N, 25° 37′E), Bulgaria are presented. During the period 1999–2003 the TOC data show seasonal variations, typical for the middle latitudes – maximum in the spring and minimum in the autumn. The comparison between TOC ground-based data and Global Ozone Monitoring Experiment (GOME) satellite-borne ones shows a seasonal dependence of the differences between them.A strong negative relationship between the total ozone and the 305 nm wavelength irradiance was found. The dependence between the two variables is significant (r = −0.62 ± 0.18) at 98% confidence level.The direct sun UV doses for some specific biological effects (erythema and eyes) are obtained. The estimation of the radiation amplification factor RAF shows that the ozone reduction by 1% increases the erythemal dose by 2.3%. The eye-damaging doses are more influenced by the TOC changes and in this case RAF = −2.7%.The amount of these biological doses depended on the solar altitude over the horizon. This dependence was not so strong when the total ozone content in the atmosphere was lower.     

Spore dosimetry of solar UV radiation: applications to monitoring of daily irradiance and personal exposure.

Environmental UV radiation can be quantified using spore dosimetry, which measures the inactivation of repair-deficient Bacillus subtilis spores dried on a membrane filter. The system exhibits highly selective sensitivity to UV radiation, not being affected by various environmental adversities, such as high and low temperature and humidity. Biologically-effective dose rate and cumulative dose of ambient radiation are measurable under various conditions at various places on the earth, including tropical, temperate, and polar sites. Applications to monitor the exposure at the surface of organisms including humans and plants have also been advanced.     

Contribution of solar UV radiation to the observed ozone variations during the 21st and 22nd solar cycles

A two-dimensional dynamical radiative-photochemical model of the ozonosphere including aerosol physics is used to examine the changes of the Earth's ozone layer occurred during the 21st and 22nd solar cycles. The calculated global total ozone changes in the latitude range 60°S—60°N caused by 11-year variation of solar UV radiation, volcanic eruptions, and anthropogenic atmospheric pollution containing CO₂, CH₄, N₂O and chlorine and bromine species are in a rather good agreement with the observed global ozone trend. The calculations show that the anthropogenic pollution of the atmosphere is a main reason of the ozone depletion observed during the last two solar cycles. However, the 11-year solar UV variation as well as volcanic eruptions of El Chichon and Mt. Pinatubo also gave a significant contribution to the observed global ozone changes.     

Reconstruction of solar UV irradiance

Understanding solar influence on the Earth’s climate requires a reconstruction of solar irradiance for the pre-satellite period. Considerable advances have been made in modelling the irradiance variations at wavelengths longer than 200 nm. At shorter wavelengths, however, the LTE approximation usually taken in such models fails, which makes a reconstruction of the solar UV irradiance a rather intricate problem. We choose an alternative approach and use the observed SUSIM UV spectra to extrapolate available models to shorter wavelengths.     

Earth life support for aquatic organisms, system and technical aspects.

The importance of the research on Bioregenerative Life Support has increased dramatically in the last decade not only with regard to possible space flight application but also as a way to obtain a better understanding of our Earth's ecology. A major goal was to reach long-term stability of artificial model systems. Preliminary data are presented on the development of an improved aquatic system, currently dedicated for ground-based research. Closed aquatic ecosystems require reliability of the key parameters of pH, O2 and CO2 concentration and stability of sensors for monitoring. Besides the integration of an artificial lung (holofiber system and air pump with valves, allowing controlled oxygen uptake of air), in parallel to the oxygen producing water plants. Our new approach is to implement opto-chemical sensors, for such environmental monitoring. One major advantage of the new sensor technique is their better long-term reliability as compared to the electrochemical sensors. Our experiment with the new sensor technique has demonstrated satisfactory performance in closed aquatic ecosystems.     

Monitoring of environmental UV radiation by biological dosimeters.

As a consequence of the stratospheric ozone layer depletion biological systems can be damaged due to increased UV-B radiation. The aim of biological dosimetry is to establish a quantitative basis for the risk assessment of the biosphere. DNA is the most important target molecule of biological systems having special sensitivity against short wavelength components of the environmental radiation. Biological dosimeters are usually simple organisms, or components of them, modeling the cellular DNA. Phage T7 and polycrystalline uracil biological dosimeters have been developed and used in our laboratory for monitoring the environmental radiation in different radiation conditions (from the polar to equatorial regions). Comparisons with Robertson-Berger (RB) meter data, as well as with model calculation data weighted by the corresponding spectral sensitivities of the dosimeters are presented. Suggestion is given how to determine the trend of the increase in the biological risk due to ozone depletion.     

Life support for aquatic species--past; present; future.

Life Support is a basic issue since manned space flight began. Not only to support astronauts and cosmonauts with the essential things to live, however, also animals which were carried for research to space etc. together with men need support systems to survive under space conditions. Most of the animals transported to space participate at the life support system of the spacecraft. However, aquatic species live in water as environment and thus need special developments. Research with aquatic animals has a long tradition in manned space flight resulting in numerous life support systems for them starting with simple plastic bags up to complex support hardware. Most of the recent developments have to be identified as part of a technological oriented system and can be described as small technospheres. As the importance arose to study our Earth as the extraordinary Biosphere we live in, the modeling of small ecosystems began as part of ecophysiological research. In parallel the investigations of Bioregenerative Life Support Systems were launched and identified as necessity for long-term space missions or traveling to Moon and Mars and beyond. This paper focus on previous developments of Life Support Systems for aquatic animals and will show future potential developments towards Bioregenerative Life Support which additionally strongly benefits to our Earth's basic understanding.     

UV photobiochemistry of anhydrobiotic organisms at extremely low temperatures

Spores of Bacillus subtilis (TKJ 3412), cells of Deinococcus radiodurans R₁ (wild type) and conidia of Aspergillus ochraceus (strain 3174) have been UV irradiated (254 nm) in the dry state (3% relative humidity, argon) or in aqueous suspension at room temperature, at −55°C to −70°C and at −165°C to −170°C. The following effects have been analyzed: decrease in viability, occurrence of DNA strand breaks (pulsed-field gel electrophoresis) and production of DNA-protein cross-links (membrane filter method). The loss in viability is usually more pronounced at around −70°C than at room temperature, but it is lowest around −170°C. The kind of prevailing DNA damage varies from organism to organism. The amount of UV induced DNA-protein cross-link products steadily decreases with the temperature and is lowest at −170°C. The decrease in highly polymeric DNA by double strand breaks follows no universal pattern. The observed hypersensitivity of the three very different species at −70°C can therefore not be simply explained on the basis of the number of DNA lesions analyzed in the course of this work. We suggest that also the changing state of cellular water below and above about −130°C significantly contributes to the change in photosensitivity.     

Effects of orbit progression on the radiation exposures from solar proton fluxes in low Earth orbit under geomagnetic storm conditions.

The present study examines the effects of orbit progression on the exposures within a Space Station Freedom module in a 51.6-degree inclined orbit at 450 km. The storm evolution is modeled after the November 1960 event, and the solar proton flux evolution is taken from the August 1972 solar proton event. The effects of a strong magnetic shock, such as was observed during the October 1989 event, is also modeled. The statistics on hourly average storm fields for the last forty years reveal that the largest geomagnetic storms approach a Dst value of -500 nanotesla at the storm peak. Similarly, one of the largest satellite-measured proton flux (> 10 MeV) for space exposures is the event of August 1972. The effects of orbit progression (advance of the line of nodes) is examined for the above conditions to study the variation of exposures under differing times of occurrence of the solar proton peak intensity, attainment of geomagnetic storm maximum, and the location of the line of nodes of the last geomagnetically protected orbit. The impact of the inherent inhomogeneity of the space station module is examined as a limiting factor on exposure with regard to the need of additional parasitic shielding.     

On the time lag between solar wind dynamic parameters and solar activity UV proxies

The solar activity displays variability and periodic behaviours over a wide range of timescales, with the presence of a most prominent cycle with a mean length of 11 years. Such variability is transported within the heliosphere by solar wind, radiation and other processes, affecting the properties of the interplanetary medium. The presence of solar activity–related periodicities is well visible in different solar wind and geomagnetic indices, although their time lags with respect to the solar cycle lead to hysteresis cycles. Here, we investigate the time lag behaviour between a physical proxy of the solar activity, the Ca II K index, and two solar wind parameters (speed and dynamic pressure), studying how their pairwise relative lags vary over almost five solar cycles. We find that the lag between Ca II K index and solar wind speed is not constant over the whole time interval investigated, with values ranging from 6 years to $～$1 year (average 3.2 years). A similar behaviour is found also for the solar wind dynamic pressure. Then, by using a Lomb-Scargle periodogram analysis we obtain a 10.21-year mean periodicity for the speed and 10.30-year for the dynamic pressure. We speculate that the different periodicities of the solar wind parameters with respect to the solar 11-year cycle may be related to the overall observed temporal evolution of the time lags. Finally, by accounting for them, we obtain empirical relations that link the amplitude of the Ca II K index to the two solar wind parameters.     

Reconstruction of the solar UV irradiance back to 1974

The variability of the solar UV irradiance has strong effects on the terrestrial atmosphere. In order to study the solar influence for times when no UV observations are available, it is necessary to reconstruct the variation of the UV irradiance with time on the basis of proxies. We present reconstructions of the solar UV irradiance based on the analysis of space-based and ground-based magnetograms of the solar disk going back to 1974. With COde for Solar Irradiance (COSI) we calculate solar intensity spectra for the quiet Sun and different active regions and combine them according to their fractional area on the solar disk, whereby their time-dependent contributions over the solar cycle lead to a variability in radiation. COSI calculates the continuum and line formation under conditions which are out of local thermodynamic equilibrium (non-LTE). The applied temperature and density structures include the chromosphere and transition region, which is particularly important for the UV. The reconstructions are compared with observations.     

UV photobiochemistry under space conditions

The response of spores of Bacillus subtilis, cells of Deinococcus radiodurans and conidia of Aspergillus ochraceus to actual and simulated space conditions (UV in combination with long-term exposure to extremely dry conditions, including vacuum) has been studied: The following effects have been analyzed: decrease of viability, occurrence of DNA double strand breaks, formation of DNA-protein cross-links and DNA-DNA cross-links. All organisms show an increased sensitivity to UV light in extreme dryness (dry argon or vacuum) compared to an irradiation in aqueous suspension. The UV irradiation leads in all cases to a variety of DNA lesions. Very conspicuous is the occurrence of double strand breaks. Most of these double strand breaks are produced by incomplete repair of other lesions, especially base damages. The increase in DNA lesions can be correlated to the loss in viability. The specific response of the chromosomal DNA to UV irradiation in extreme dryness, however, varies from species to species and depends on the state of dehydration. The formation of DNA double strand breaks and DNA-protein cross-links prevails in the case of B. subtilis spores. In cells of Deinococcus radiodurans DNA-DNA cross-links often predominate, in conidia of Aspergillus ochraceus double strand breaks. The results obtained by direct exposure to space conditions (EURECA mission and D2 mission) largely agree with the laboratory data.     

Sailing with solar and planetary radiation pressure

Literature on solar sailing has thus far mostly considered solar radiation pressure (SRP) as the only contribution to sail force. However, considering a sail in a planetary mission scenario, a new contribution can be added. Since the planet itself emits radiation, this generates a radial planetary radiation pressure (PRP) that is also exerted on the sail. Hence, this work studies the combined effects of both SRP and PRP on a sail for two case studies, i.e. Earth and Venus. In proximity of the Earth, the effect of PRP can be significant under specific conditions. Around Venus, instead, PRP is by far the dominating contribution. These combined effects have been studied for single- and double-sided reflective coating and including eclipse. Results show potential increase in the net acceleration and a change in the optimal attitude to maximise the acceleration in a given direction. Moreover, an increasing semi-major axis manoeuvre is shown with and without PRP, to quantify the difference on a real-case scenario.     

Experimental investigation of transient thermal behavior of an airship under different solar radiation and airflow conditions

Knowledge of the thermal behavior of airships is crucial to the development of airship technology. An experiment apparatus is constructed to investigate the thermal response characteristics of airships, and the transient temperature distributions of both hull and inner gas are obtained under the irradiation of a solar simulator and various airflow conditions. In the course of the research, the transient temperature change of the experimental airship is measured for four airflow speeds of 0 m/s (natural convection), 3.26 m/s, 5.5 m/s and 7.0 m/s, and two incident solar radiation values of 842.4 W/m² and 972.0 W/m². The results show that solar irradiation has significant influence on the airship hull and inner gas temperatures even if the airship stays in a ground airflow environment where the heat transfer is dominated by radiation and convection. The airflow around the airship is conducive to reduce the hull temperature and temperature nonuniformity. Transient thermal response of airships rapidly varies with time under solar radiation conditions and the hull temperature remains approximately constant in ∼5–10 min. Finally, a transient thermal model of airship is developed and the model is validated through comparison with the experimental data.     

Analysis of radiation risk from alpha particle component of solar particle events.

The solar particle events (SPE) will contain a primary alpha particle component, representing a possible increase in the potential risk to astronauts during an SPE over the often studied proton component. We discuss the physical interactions of alpha particles important in describing the transport of these particles through spacecraft and body shielding. Models of light ion reactions are presented and their effects on energy and linear energy transfer (LET) spectra in shielding discussed. We present predictions of particle spectra, dose, and dose equivalent in organs of interest for SPE spectra typical of those occurring in recent solar cycles. The large events of solar cycle 19 are found to have substantial increase in biological risk from alpha particles, including a large increase in secondary neutron production from alpha particle breakup.     

Observation bio-effect of SW-global solar radiation in Ilorin in the tropics

The annual variation of global (total) solar radiation measured over four (4) years (1995–1998) at Ilorin (8°32′N, 4°34′E) was studied and the bio-effects of the variation is herein reported. The weekly averages of the solar radiation flux were obtained and plotted. The profile of the weekly averages of the insolation shows two notable “wells” corresponding to the harmattan dry season and the rainy season. It was deduced that the duration of the seasons was directly proportional to the sizes of the “wells”, while the depths of the “wells” correspond to the severity of the causes of the “wells”. The fall and rise rates of the depth of the edges of the wells are inversely proportional to the ecological effects of the variation of the radiation. The rates of decrease and increase of the radiation flux affect the depths of the “wells” and were found to be rapid and shallow for the “Harmattan Well”, and gentle and deep for the “Rain Well”. The width (duration), rate of change and depth of the “Harmattan Well” bring about stress for both animals and plants.     

Impact linear polarization observed in a UV chromospheric line during a solar flare

Linear polarization was observed in the S I 1437Å line in bright flaring points during the soft X-ray emission. The degree of polarization is about 25% and is detected at a signal to noise ratio of 2.9. The polarized electric vector is directed towards disk center to within 3°.This polarization could be due to collisional excitationm of S I by energetic electrons beamed in the vertical direction. We suggest that the heat flux in the region interconnecting the transition zone to the high chromosphere during the gradual phase of a flare could lead to an anisotropic excitation. Then the observed polarization would be due to vertical motions of the transition zone sweeping the preexisting chromosphere.     

Repair of radiation induced genetic damage under microgravity.

The influence of microgravity on the repair of radiation induced genetic damage in a temperature-conditional repair mutant of the yeast Saccharomyces cerevisiae (rad 54-3) was investigated onboard the IML-1 mission (January 22nd-30th 1992, STS-42). Cells were irradiated before the flight, incubated under microgravity at the permissive (22 degrees C) and restrictive (36 degrees C) temperature and afterwards tested for survival. The results suggest that repair may be reduced under microgravity.