Regeneration of organs and tissues in lower vertebrates during and after space flight.

In this paper most important data obtained in studies on the effect of space flight conditions on regeneration in the adult newt are summarized. We demonstrate a phenomenon of synchronization of limb and lens regeneration and increase in its rate during and after space flight. We also describe a peculiarities of cell proliferation in lens, limb and tail regenerates and of the process of minced muscle regeneration.     

The pleurodele, an animal model for space biology studies.

Pleurodeles waltl, an Urodele amphibian is proposed as a model for space biology studies. Our laboratory is developing three types of experiments in space using this animal: 1) in vivo fertilization and development ("FERTILE" project); 2) influence of microgravity and space radiation on the organization and preservation of specialized structures in the neurons and muscle cells (in vitro; "CELIMENE" PROJECT); 3) influence of microgravity on tissue regeneration (muscle, bone, epidermis and spinal cord).     

Regeneration of guinea pig facial nerve: the effect of hypergravity.

Exposure to moderate hypergravity improves the regenerative capacity of sectioned guinea-pig facial nerve. The improvement in regeneration is tri-directional as follows: a) an average 1.7 fold increase in rate of regeneration in guinea pigs subjected to hypergravity; b) a 25% enhancement of facial muscle activity following the exposure to hypergravity; and c) improvement in the quality of regeneration from an esthetic standpoint. A good correlation was recorded between the histological structure of the severed nerve at the end of the regeneration and the clinical results.     

Effects of microgravity on muscle and cerebral cortex: a suggested interaction.

The "slow" antigravity muscle adductor longus was studied in rats after 14 days of spaceflight (SF). The techniques employed included standard methods for light microscopy, neural cell adhesion molecule (N-CAM) immunocytochemistry and electron microscopy. Light and electron microscopy revealed myofiber atrophy, segmental necrosis and regenerative myofibers. Regenerative myofibers were N-CAM immunoreactive (N-CAM-IR). The neuromuscular junctions showed axon terminals with a decrease or absence of synaptic vesicles, degenerative changes, vacant axonal spaces and changes suggestive of axonal sprouting. No alterations of muscle spindles was seen either by light or electron microscopy. These observations suggest that muscle regeneration and denervation and synaptic remodeling at the level of the neuromuscular junction may take place during spaceflight. In a separate study, GABA immunoreactivity (GABA-IR) was evaluated at the level of the hindlimb representation of the rat somatosensory cortex after 14 days of hindlimb unloading by tail suspension ("simulated" microgravity). A reduction in number of GABA-immunoreactive cells with respect to the control animals was observed in layer Va and Vb. GABA-IR terminals were also reduced in the same layers, particularly those terminals surrounding the soma and apical dendrites of pyramidal cells in layer Vb. On the basis of previous morphological and behavioral studies of the neuromuscular system after spaceflight and hindlimb suspension it is suggested that after limb unloading there are alterations of afferent signaling and feedback information from intramuscular receptors to the cerebral cortex due to modifications in the reflex organization of hindlimb muscle groups. We propose that the changes observed in GABA immunoreactivity of cells and terminals is an expression of changes in their modulatory activity to compensate for the alterations in the afferent information.     

Urodelean amphibians in studies on microgravity: effects upon organ and tissue regeneration.

Results obtained from nine experiments performed onboard Russian biosatellites have shown that microgravity promotes tissue regeneration in the newt, Pleurodeles waltl. The effect has been reproduced in all flights and on a clinostat as well for eye tissues (lens and retina), limbs and tail. The effect was demonstrated in 1.5- to 2-fold increase in cell proliferation in the early stages of regeneration in space flight. Animals "flown" intact and operated after flight regenerated faster than control ones and showed long-lasting micro-"g" effect. The most recent experiment flew aboard the Bion-11 biosatellite. This test was performed for study on microgravity effect on neural retina regeneration after optic nerve lesioning in the newt. Obtained results confirmed our previous information about intensification of regenerative processes in detached neural retina in urodela exposed to simulated weightlessness (Grigoryan et al., 1998). In particular, we found the increase and activation of cell populations participating in neural retina restoration and maintenance of retinal structure. Our findings suggest that promoting effect of microgravity upon regeneration could be influenced by several factors, largely influenced by a response of the whole organism to changed gravity vector. We hypothesized the synthesis of the specific range of stress proteins induced by micro-"g" and their regulative role in cell proliferation. Such a hypothesis for the existence of "altered gravity stress proteins" is discussed.     

The effect of microgravity on the development of plant protoplasts flown on Biokosmos 9.

An experiment using plant protoplasts has been accepted for the IML-1 Space Shuttle mission scheduled for 1991. Preparatory experiments have been performed using both fast and slow rotating clinostats and in orbit to study the effect of simulated and real weightlessness on protoplast regeneration. Late access to the space vehicles before launch has required special attention since it is important to delay cell wall regeneration until the samples are in orbit. On a flight on Biokosmos 9 ("Kosmos-2044") in September 1989 some preliminary results were obtained. Compared to the ground control, the growth of both carrot and rapeseed protoplasts was decreased by 18% and 44% respectively, after 14 days in orbit. The results also indicated that there is less cell wall regeneration under micro-g conditions. Compared to the ground controls the production of cellulose in rapeseed and carrot flight samples was only 46% and 29% respectively. The production of hemicellulose in the flight samples was 63% and 67% respectively of that of the ground controls. In both cases all samples reached the stage of callus development. The peroxidase activity was also found to be lower in the flight samples than in the ground controls, and the number of different isoenzymes was decreased in the flight samples. In general, the regeneration processes were retarded in the flight samples with respect to the ground controls. From a simulation experiment for IML-1 performed in January 1990 at ESTEC, Holland, regenerated plants have been obtained. These results are discussed and compared to the results obtained on Biokosmos 9. Protoplast regeneration did not develop beyond the callus stage in either the flight or the ground control samples from the Biokosmos 9 experiment.     

Influence of clinorotation and fettering stress on tail regeneration of Triturus vulgaris (Urodela).

Tail-amputated adult Triturus vulgaris, fettered in cuvettes of a fast-rotating clinostat were exposed to simulated weightlessness (60 rpm; equiv. to 10(-3)-10(-4) g), during a 14-day period. To feed and clean the animals rotation was stopped once a day for approx. 10 min. To test the influence of the fettering stress, a second series of animals was kept separately under normal earth conditions without rotation. A further control series was kept in a dark container without any handicap. While tail regeneration of the rotated animals was markedly accelerated, the fettered-only animals showed a considerably less marked acceleration effect. At the end of the 14-day period, all regenerates were reamputated together with an additional 5 mm of the tail stump. Although this second level of amputation was distant from the first, the regenerative growth rate of the rotated series was accelerated 123% in contrast to both the control and the fettered-only series. Our results demonstrate that the growth acceleration is induced by clinorotation. Fettering stress has no comparable influence. The growth promoting effect is not limited to the regenerating area.     

Microgravity effects on neural retina regeneration in the newt.

Data on forelimb and eye lens regeneration in urodeles under spaceflight conditions (SFC) have been obtained in our previous studies. Today, evidence is available that SFC stimulate regeneration in experimental animals rather than inhibit it. The results of control on-ground experiments with simulated microgravity suggest that the stimulatory effect of SFC is due largely to weightlessness. An original experimental model is proposed, which is convenient for comprehensively analyzing neural regeneration under SFC. The initial results described here concern regeneration of neural retina in Pleurodeles waltl newts exposed to microgravity simulated in radial clinostat. After clinorotation for seven days (until postoperation day 16), a positive effect of altered gravity on structural restoration of detached neural retina was confirmed by a number of criteria. Specifically, an increased number of Mullerian glial cells, an increased relative volume of the plexiform layers, reduced cell death, advanced redifferentiation of retinal pigment epithelium, and extended areas of neural retina reattachment were detected in experimental newts. Moreover, cell proliferation in the inner nuclear layer of neural retina increased as compared with control. Thus, low gravity appears to intensify natural cytological and molecular mechanisms of neural retina regeneration in lower vertebrates.     

Signs of Müller cell gliotic response found in the retina of newts exposed to real and simulated microgravity

The effects of real and simulated microgravity on the eye tissue regeneration of newts were investigated. For the first time changes in Müller glial cells in the retina of eyes regenerating after retinal detachment were detected in newts exposed to clinorotation. The cells divided, were hypertrophied, and their processes were thickened. Such changes suggested reactive gliosis and were more significant in animals exposed to rotation when compared with desk-top controls. Later experiments onboard the Russian biosatellite Bion-11 showed similar changes in the retinas that were regenerating in a two-week spaceflight. In the Bion-11 animals, GFAP, the major structural protein of retinal macroglial cells, was found to be upregulated. In a more recent experiment onboard Foton-M3 (2007), GFAP expression in retinas of space-flown, ground control (kept at 1 g), and basal control (sacrificed on launch day) newts was quantified, using microscopy, immunohistochemistry, and digital image analysis. A low level of immunoreactivity was observed in basal controls. In contrast, retinas of space-flown animals showed greater GFAP immunoreactivity associated with both an increased cell number and a higher thickness of intermediate filaments. This, in turn, was accompanied by up-regulation of stress protein (HSP90) and growth factor (FGF2) expressions. It can be postulated that such a response of Müller cells was to mitigate the retinal stress in newts exposed to microgravity. Taken together, the data suggest that the retinal population of macroglial cells could be sensitive to gravity changes and that in space it can react by enhancing its neuroprotective function.     

Inconsistency of magnetic field and plasma velocity variations in the distant plasma sheet: Violation of the “frozen-in” criterion?

Measurements of the magnetic field and low energy plasma by the GEOTAIL spacecraft have been used to study the relationship between variations of the plasma velocity and of the magnetic field in the distant (100–200 R_E) and middle (40–80 R_E) tail. The analysis was carried out separately for the tail lobes and the plasma sheet. It is shown that the absolute values of the magnetic field and plasma velocity, as well as their corresponding components (V_X and B_X, V_Y and B_Y, V_Z and B_Z), are linearly connected in the tail lobes. In the plasma sheet, however, the plasma velocity and the magnetic field do not seem to be related to one another. The distant plasma sheet seems to be in a regime of turbulence. The diffusion coefficients estimated from our data set of the velocity parameters in the plasma sheet are in good agreement with the theoretical predictions of Antonova and Ovchinnikov (1996, 1999).     

基于双CAN总线的数字伺服通信协议

分析了目前国外先进数控系统现场总线的通信特性,提出和定义了一种基于双CAN(Controller Area Network)总线的数控系统位置控制通信协议——CANsmc(CAN for Synchronous Motion Control).CANsmc采用主从式的双通道网络,由一个主站和最多61个从站组成,实现了带有广播同步电报的轮询式通信.协议以通用的CAN总线芯片为硬件基础,利用CAN芯片通信报文的描述符定义出CANsmc的通信报头.CANsmc共定义了3种电报类型:主站同步电报MST,主站数据电报MDT和伺服电报DT.主站通过主通道向从站发送MST和MDT,从站在收到MDT后通过从通道向主站反馈DT.最后,建立了一个CANsmc实验验证系统,用软件的方式实现了CANsmc协议,主站基于PC机ISA总线,从站是一个位控卡,可以实现位置、速度、回参考点等多种控制.实验证明,CANsmc总线可以满足先进工业机器人和数控机床控制系统的应用要求.      

Optical observations of ion tail structures and velocity fields in comet Giacobini-Zinner near the time of the ICE encounter

Observations of constituents of the neutral coma (CN. C₂. CH. O. H) of Giacobini-Zinner were made for a period of nearly three weeks during late August/early September 1985 from La Palma Observatory, Canaries Is. in addition to studies of structures and flows in the ion coma and tail. The neutral coma (in CN) was observed to extend to a radius of at least 400,000 Km, far beyond the “bow wave” identified by the ICE spacecraft. The ion coma (detected to a sunward distance of about 50,000 Km) and ion tail fan (max. length about 500,000 Km, recorded in CO⁺ and H₂O⁺ were also observed throughout the period before and after the ICE encounter. An extended Type I ion tail central condensation was not observed. The maximum observed extent of the ‘ionospheric tail’ was about 50,000 Km, five hours prior to the ICE encounter. This ionospheric tail rapidly diffused into a broad tail fan.     

On the large-scale structure of the tail current as measured by THEMIS

The magnetic field structure and the spatial characteristics of the large-scale currents in the magnetospheric tail were studied during quiet and moderately disturbed geomagnetic conditions in 2009. The magnetic field of the currents other than the tail current was calculated in terms of a paraboloid model of the Earth’s magnetosphere, A2000, and was subtracted from measurements. It was found on the base of obtained tail current magnetic field radial distribution that the inner edge of the tail current sheet is located in the night side magnetosphere, at distances of about 10 R_E and of about 7 R_E during quiet and disturbed periods respectively. During the disturbance of February 14, 2009 (Dst_min ∼ −35 nT), the B_x and the B_z component of the tail current magnetic field near its inner edge were about 60 nT, and −60 nT that means that strong cross-tail current have been developed. The tail current parameters at different time moments during February 14, 2009 have been estimated. Solar wind conditions during this event were consistent with those during moderate magnetic storms with minimum Dst of about −100 nT. However, the magnetospheric current systems (magnetopause and cross-tail currents) were located at larger geocentric distances than typical during the 2009 extremely quiet epoch and did not provide the expected Dst magnitude. Very small disturbance on the Earth’s surface was detected consistent with an “inflated” magnetosphere.     

Effects of clinorotation on the polysaccharide content of resynthesized walls of protoplasts.

Changes in cellulose and callose content during cell wall regeneration in Brassica oleracea protoplasts have been examined by cytofluorimetry following their exposure to the conditions of the horizontal clinostat (2 r.p.m.) for 10 days. In comparison with controls, cellulose content decreased 4-fold and 28% of the protoplasts failed to resynthesize a wall in the clinorotated sample. The callose content was almost doubled in clinostated cells. Callose synthesis fluctuated in both control and clinorotated protoplasts. The results support the idea that inhibition of cellulose synthesis in protoplasts grown on the clinostat is caused by a change of plasmalemma fluidity and functioning, and also by a disturbance to the state of cytoplasmic calcium under conditions of simulated microgravity.     

Influence of longitudinal whole animal clinorotation on lens, tail, and limb regeneration in urodeles.

Two species of newts (Urodela) and two types of clinostats for fast clinorotation (60 rpm) were used to investigate the influence of simulated weightlessness on regeneration and to compare results obtained with data from spaceflight experiments. Seven or fourteen days of weightlessness in Russian biosatellites caused acceleration of lens and limb regeneration by an increase in cell proliferation, differentiation, and rate of morphogenesis in comparison with ground controls. After a comparable time of clinorotation the results obtained with Triturus vulgaris using a horizontal clinostat were similar to those found in spaceflight. In contrast, in Pleurodeles waltl using both horizontal and radial clinostats the results were contradictory compared to Triturus. We speculate that different levels of gravity or/and species specific thresholds for gravitational sensitivity could be responsible for these contradictory results.     

一种模拟长期失重影响的大鼠尾部悬吊模型

本文改进了Morey-Holton和wronski的大鼠尾部悬吊方法,还总结出了一套不致对尾部造成损伤的长期尾部悬吊技术。用此方法,完成了五批(每批10只)为期90—120天的悬吊实验。结果表明:引起的应激程度轻微;悬吊组大鼠体重增长与对照大鼠接近。可用来对长期失重生理影响及其机理进行研究。     

Magnetotail current contribution to the Dst Index Using the MT Index and the WINDMI model

In this paper, we have improved the capabilities of a low dimensional nonlinear dynamical model called WINDMI to determine the state of the global magnetosphere by employing the magnetotail (MT) index as a measurement constraint during large geomagnetic storms. The MT index is derived from particle precipitation measurements made by the NOAA/POES satellites. This index indicates the location of the nightside ion isotropic boundary, which is then used as a proxy for the strength of the magnetotail current in the magnetosphere. In Asikainen et al. (2010), the contribution of the tail current to the Dst index is estimated from an empirical relationship based on the MT index. Here the WINDMI model is used as a substitute to arrive at the tail current and ring current contribution to the Dst index, for comparison purposes. We run the WINDMI model on 7 large geomagnetic storms, while optimizing the model state variables against the Dst index, the MT index, and the AL index simultaneously. Our results show that the contribution from the geotail current produced by the WINDMI model and the MT index are strongly correlated, except during some periods when storm time substorms are observed. The inclusion of the MT index as an optimization constraint in turn increases our confidence that the ring current contribution to the Dst index calculated by the WINDMI model is correct during large geomagnetic storms.     

喷口布局对导弹侧向喷流控制作用的数值模拟

为保持飞行器在稀薄大气中的机动性,通常采用喷流反控制作用(RCS),但在超声速来流中,这会导致飞行器表面出现复杂的喷流干扰流场,对飞行控制造成了巨大影响。为提高对超声速条件下的侧向喷流控制作用的规律性认识,应用数值模拟方法,研究了超声速条件下的无舵光滑弹体和带尾舵的弹-翼组合体上的声速侧向喷流控制问题。开展了关于喷口布局对侧向喷流控制效果影响规律的研究工作,并通过引入法向干扰力沿程增长系数从定量角度加以分析。计算结果表明:在有尾舵的情况下,喷口位置的后移和马赫数的增加能够显著增强侧向喷流控制效果;当喷口位置位于舵面之前时,喷流干扰力放大系数随迎角增大而增大,随来流静压增大而减小;当喷流位置后移至舵面之后时,规律相反;在某些喷口位置和来流条件下,弹-翼组合体的侧向控制效果与无舵光滑弹体相比并不具备优势。     

充磁导致的超高速永磁同步电机不平衡磁拉力

转子永磁体充磁角度偏差导致的不平衡磁拉力对微型燃气轮机发电系统用超高速永磁同步电机的安全稳定运行有很大的影响.采用有限元数值分析方法,分别在不考虑转子偏心和考虑转子偏心的情况下,对由充磁角度偏差导致的超高速永磁同步电机不平衡磁拉力进行了计算和分析;将不平衡磁拉力的计算结果作为载荷,获得了超高速永磁同步电机空气轴承-转子系统的固有频率及振动特性.研究结果表明:转子系统在740 Hz附近发生共振,共振幅值随着转子磁芯充磁偏差角度的增大而增大;要保证转子系统安全可靠的工作,需要将磁芯的充磁角度偏差限制在5°以内.     

Pc5 Oscillation Analysis by the Satellite and Ground-Based Data

Large amplitude Pc5 event was observed in the space and on ground on August 3, 2001, about three hours after contact of the strong discontinuity in the solar wind with the magnetosphere according to data from ACE and Wind satellites. The Pc5 amplitude was as high as 15 nT in the tail of magnetosphere and about 5 nT at the ground based stations. In the magnetosphere Pc5 waves were observed by Cluster and Polar satellites, which occupied positions in the morning part of the near tail at the close field lines but were parted by distance of 11.5 Re, mainly along the x-axis of the GSM coordinate system. Both compressional and transverse components of the Pc5 wave activity were observed in the space, with the transverse component having the larger amplitude. Time delay between the Cluster and Polar satellites was about 8 minutes, which could be interpreted as a wave propagation from the geomagnetic tail to the Earth with the 150 km/s group velocity. The ground-based Pc5 activity was analysed by using data from the Image magnetometer network. Doubtless demonstrations of a field line resonant structure were found in variations of amplitude and polarization with latitude. Finnish chain of search coil magnetometers observed modulated Pc1 emission simultaneously with the Pc5 wave train. A possibility of non-linear impact of Pc5 wave energy on the plasma and waves in the magnetosphere is discussed.