Changes in hyperspectral reflectance signatures of lettuce leaves in response to macronutrient deficiencies

The adaptation of specific remote sensing and hyperspectral analysis techniques for the determination of incipient nutrient stress in plants could allow early detection and precision supplementation for remediation, important considerations for minimizing mass of advanced life support systems on space station and long term missions. This experiment was conducted to determine if hyperspectral reflectance could be used to detect nutrient stress in Lactuca sativa L. cv. Black Seeded Simpson. Lettuce seedlings were grown for 90 days in a greenhouse or growth chamber in vermiculite containing modified Hoagland’s nutrient solution with key macronutrient elements removed in order to induce a range of nutrient stresses, including nitrogen, phosphorus, potassium, calcium, and magnesium. Leaf tissue nutrient concentrations were compared with corresponding spectral reflectances taken at the end of 90 days. Spectral reflectances varied with growing location, position on the leaf, and nutrient deficiency treatment. Spectral responses of lettuce leaves under macronutrient deficiency conditions showed an increase in reflectance in the red, near red, and infrared wavelength ranges. The data obtained suggest that spectral reflectance shows the potential as a diagnostic tool in predicting nutrient deficiencies in general. Overlapping of spectral signatures makes the use of wavelengths of narrow bandwidths or individual bands for the discrimination of specific nutrient stresses difficult without further data processing.     

Space Materials Science in China: II. Ground-based Researches and Academic Activities

Activities of space materials science research in China have been continuously supported by two main national programs. One is the China Space Station (CSS) program since 1992, and the other is the Strategic Priority Program (SPP) on Space Science since 2011. In CSS plan in 2019, eleven space materials science experimental projects were officially approved for execution during the construction of the space station. In the SPP Phase II launched in 2018, seven pre-research projects are deployed as the first batch in 2018, and one concept study project in 2019. These pre-research projects will be cultivated as candidates for future selection as space experiment projects on the recovery of scientific experimental satellites in the future. A new apparatus of electrostatic levitation system for ground-based research of space materials science and rapid solidification research has been developed under the support of the National Natural Science Foundation of China. In order to promote domestic academic activities and to enhance the advancement of space materials science in China, the Space Materials Science and Technology Division belong to the Chinese Materials Research Society was established in 2019. We also organized scientists to write five review papers on space materials science as a special topic published in the journal Scientia Sinica to provide valuable scientific and technical references for Chinese researchers.      

Living plant systems: How robust are they in the absence of gravity?

The following hierarchical levels can be recognised in plant systems: cells, organs, organisms and gamodemes (interbreeding members of a community). Each level in this ‘living hierarchy’ is both defined and supported by a similar set of sub-systems. Within this framework of plant organization, two complementary questions are relevant for interpreting plant-oriented space experiments: 1) What role, if any, does gravity play in enabling the development of each organizational level? and 2) Does abnormal development in an altered gravity environment indicate sub-system inefficiency? Although a few representatives of the various organizational levels in plant systems have already been the subject of microgravity experiments in space laboratories—from cells in culture to gamodemes, the latter being found in some Closed Environment Life Support Systems—it would be of interest to investigate additional systems with respect to their response to microgravity. Recognition of the sub-systems at each level might be relevant not only for a more complete understanding of plant development but also for the successful cultivation and propagation of plants during long-term space flights and the establishment of plants in extra-terrestrial environments.     

Solar wind interaction with comet Halley

In March 6 and 9, 1986 the spacecrafts ‘Vega-1’ and ‘Vega-2’ have flown through the coma of comet Halley and have carried measurements of plasma, energetic particles, magnetic field and plasma waves along its trajectory. A short review of these measurements and its comparison with theoretical models of solar wind interaction with comets are given.

The spacecrafts ‘Vega-1’ and ‘Vega-2’ have studied the solar wind loading by cometary ions, the structure of cometary bow shock and the processes in the inner coma of comet Halley. Exactly in this sequence we discuss the results of measurements and compare them with the theory.     

Distribution of energetic particles and secondary radiation according to orbital station “MIR” data obtained in 1991

A set of instruments for measuring energetic particle fluxes, containing two neutron detectors under different plexiglas shielding thicknesses, a scintillation detector, measuring energy release >0. I MeV and 0.5 MeV and a Geiger counter were launched onboard OS ‘MIR’. The latitude dependencies of the cosmic ray measurements were obtained and studied. The distributions of primary particle fluxes (protons and electrons) as well as secondary particle fluxes (bremsstrahlung gamma-rays and neutrons) produced in interactions of radiation belt particles with the station materials were obtained. The electron belt, generated during the storm of March 24 1991, is studied.     

The occurrence of single hard X-raysources in solar flares

The ‘standard’ thick target flare model predicts the existence of strong hard X-ray emission at the footpointsof a flare loop. However, Yohkoh observations suggest that up to 20% of events with data available in three or more Hard X-ray Telescope (HXT) channels show only a single source. Combining datasets from Yohkoh, the Solar and Heliospheric Observatory (SOHO), and Nobeyama Radio Heliograph (NoRH), we compare the characteristics of these single source events to double source events. The objective of this study is to determine whether these represent unresolved double footpoints, asymmetric electron deposition due to magnetic mirroring effects, or a genuine departure from the ‘standard’ model.     

Growth restoration in azuki bean and maize seedlings by removal of hypergravity stimuli.

Hypergravity stimuli, gravitational acceleration of more than 1 x g, decrease the growth rate of azuki bean epicotyls and maize coleoptiles and mesocotyls by decreasing the cell wall extensibility via an increase in the molecular mass of matrix polysaccharides. An increase in the pH in the apoplastic fluid is hypothesized to be involved in the processes of the increase in the molecular mass of matrix polysaccharides due to hypergravity. However, whether such physiological changes by hypergravity are induced by normal physiological responses or caused by physiological damages have not been elucidated. In the present study, we examined the effects of the removal of hypergravity stimuli on growth and the cell wall properties of azuki bean and maize seedlings to clarify whether the effects of hypergravity stimuli on growth and the cell wall properties are reversible or irreversible. When the seedlings grown under hypergravity conditions at 300 x g for several hours were transferred to 1 x g conditions, the growth rate of azuki bean epicotyls and maize coleoptiles and mesocotyls greatly increased within a few hours. The recovery of growth rate of these organs was accompanied by an immediate increase in the cell wall extensibility, a decrease in the molecular mass of matrix polysaccharides, and an increase in matrix polysaccharide-degrading activities. The apoplastic pH also decreased promptly upon the removal of hypergravity stimuli. These results suggest that plants regulate the growth rate of shoots reversibly in response to hypergravity stimuli by changing the cell wall properties, by which they adapt themselves to different gravity conditions. This study also revealed that changes in growth and the cell wall properties under hypergravity conditions could be recognized as normal physiological responses of plants. In addition, the results suggest that the effects of microgravity on plant growth and cell wall properties should be reversible and could disappear promptly when plants are transferred from microgravity to 1 x g. Therefore, plant materials should be fixed or frozen on orbit for detecting microgravity-induced changes in physiological parameters after recovering the materials to earth in space experiments.     

ROSAT observations of the Chamaeleon star forming cloud

Two soft X-ray images of the Chamaeleon I star forming cloud obtained with the ROSAT Position Sensitive Proportional Counter are presented. Seventy reliable, and perhaps 19 additional, X-ray sources are found. Up to Ninety percent of these sources are certainly or probably identified with T Tauri stars formed in the cloud. Twenty to 35 are probably previously unrecognized ‘weak’ T Tauri (WTT) stars. T Tauri X-ray luminosities range from log , or 10² – 10⁴ times solar levels, with mean in the 0.2–2.5 keV band. The X-ray luminosities of well-studied Chamaeleon cloud members are correlated with a complex of four stellar properties: effective temperature, mass, radius and bolometric luminosity. The spatial distribution, H-R diagram locations of the stars indicate WTT and CTT are coeval. The total premain sequence population of the cloud is likely to be > 100 stars, with WTT stars outnumbering ‘classical’ T Tauri (CTT) stars by 2:1.     

Gamma rays and cosmic ray gradiants

The advent of data from the Gamma Ray Observatory, of much higher precision than hitherto, will open up the possibility of deriving more precise results on the presence of ‘cosmic ray gradients’ in the Galaxy. There will still be a variety of difficulties, however. There are still uncertainties in the mass of gas in each of its major forms, HI, H₂ and HII, and their corresponding spatial distribution and the photon field in the Galaxy are known imprecisely. The problem of the contribution to the gamma ray flux from unresolved discrete sources will still be present, too, albeit at a lower level than previously. The division of parent particles between electrons and protons (nuclei) will also continue to be rather uncertain.

These problems are examined in the paper.     

Update on Fengyun Meteorological Satellite Program and Development

China began to develop its meteorological satellite program since 1969. With 50-years' growing, there are 17 Fengyun (FY) meteorological satellites launched successfully. At present, seven of them are in orbit to provide the operational service, including three polar orbiting meteorological satellites and four geostationary meteorological satellites. Since last COSPAR report, no new Fengyun satellite has been launched. The information of the on-orbit FY-2 series, FY-3 series, and FY-4 series has been updated. FY-3D and FY-2H satellites accomplished the commission test and transitioned into operation in 2018. FY-2E satellite completed its service to decommission in 2019. The web-based users and Direct Broadcasting (DB) users keep growing worldwide to require the Fengyun satellite data and products. A new Mobile Application Service has been launched to Fengyun users based on the cloud technology in 2018. In this report, the international and regional co-operations to facilitate the Fengyun user community have been addressed especially. To strengthen the data service in the Belt and Road countries, the Emergency Support Mechanism of Fengyun satellite (FY_ESM) has been established since 2018. Meanwhile, a Recalibrating 30-years' archived Fengyun satellite data project has been founded since 2018. This project targets to generate the Fundamental Climate Data Record (FCDR) as a space agency response to the Global Climate Observation System (GCOS). At last, the future Fengyun program up to 2025 has been introduced as well.      

微重力条件下红外材料的空间生长

根据近年红外材料在空间生长的研究概况,分析了涵盖熔体、气相、液相外延和分子束外延等生长方法及各个方法生长红外材料的基本原理,在地面生长遇到的问题,以及在空间微重力环境中进行红外材料生长的优势.同时基于中国利用熔体法在天宫二号上已经进行的ZnTe:Cu晶体的空间生长科学实验及地基实验结果,通过对空间和地面实验结果进行对比,提出了在超高真空环境下,利用分子束外延方式生长高性能、大尺寸红外材料的可能性,以及未来在空间微重力条件下进行红外材料生长的研究方向及具体应用.      

Biological dosimetry to determine the UV radiation climate inside the MIR station and its role in vitamin D biosynthesis

The vitamin D synthesis in the human skin, is absolutely dependent on UVB radiation. Natural UVB from sunlight is normally absent in the closed environment of a space station like MIR. Therefore it was necessary to investigate the UV radiation climate inside the station resulting from different lamps as well as from occasional solar irradiation behind a UV-transparent quartz window. Biofilms, biologically weighting and integrating UV dosimeters successfully applied on Earth (e.g. in Antarctica) and in space (D-2, Biopan I) were used to determine the biological effectiveness of the UV radiation climate at different locations in the space station. Biofilms were also used to determine the personal UV dose of an individual cosmonaut. These UV data were correlated with the concentration of vitamin D in the cosmonaut's blood and the dietary vitamin D intake. The results showed that the UV radiation climate inside the Mir station is not sufficient for an adequate supply of vitamin D, which should therefore be secured either by vitamin D supplementat and/or by the regular exposure to special UV lamps like those in sun-beds. The use of natural solar UV radiation through the quartz window for ‘sunbathing’ is dangerous and should be avoided even for short exposure periods.     

Biological effects of weightlessness and clinostatic conditions registered in cells of root meristem and cap of higher plants.

Research in cellular reproduction, differentiation and vital activity, i.e. processes underlying the development and functioning of organisms, plants included, is essential for solving fundamental and applied problems of space biology. Detailed anatomical analysis of roots of higher plants grown on board the Salyut 6 orbital research station show that under conditions of weightlessness for defined duration mitosis, cytokinesis and tissue differentiation in plant vegetative organs occur essentially normally. At the same time, certain rearrangements in the structural organization of cellular organelles--mainly the plastid apparatus, mitochondria, Golgi apparatus and nucleus--are established in the root meristem and cap of the experimental plants. This is evidence for considerable changes in cellular metabolism. The structural changes in the subcellular level arising under spaceflight conditions are partially absent in clinostat experiments designed to simulate weightlessness. Various clinostatic conditions have different influences on the cell structural and functional organization than does space flight. It is suggested that alterations of cellular metabolism under weightlessness and clinostatic conditions occur within existing genetic programs.     

Effects of mineral nutrition conditions on heat tolerance of chufa (Сyperus esculentus L.) plant communities to super optimal air temperatures in the BTLSS

The use of mineralized human wastes as a basis for nutrient solutions will increase the degree of material closure of bio-technical human life support systems. As stress tolerance of plants is determined, among other factors, by the conditions under which they have been grown before exposure to a stressor, the purpose of the study is to investigate the level of tolerance of chufa (Cyperus esculentus L.) plant communities grown in solutions based on mineralized human wastes to a damaging air temperature, 45 °C. Experiments were performed with 30-day-old chufa plant communities grown hydroponically, on expanded clay aggregate, under artificial light, at 690 μmol m⁻² s⁻¹ PAR and at a temperature of 25 °C. Plants were grown in Knop’s solution and solutions based on human wastes mineralized according to Yu.A. Kudenko’s method, which contained nitrogen either as ammonium and urea or as nitrates. The heat shock treatment lasted 20 h at 690 and 1150 μmol m⁻² s⁻¹ PAR. Chufa heat tolerance was evaluated based on parameters of CO₂ gas exchange, the state of its photosynthetic apparatus (PSA), and intensity of peroxidation of leaf lipids. Chufa plants grown in the solutions based on mineralized human wastes that contained ammonium and urea had lower heat tolerance than plants grown in standard mineral solutions. Heat tolerance of the plants grown in the solutions based on mineralized human wastes that mainly contained nitrate nitrogen was insignificantly different from the heat tolerance of the plants grown in standard mineral solutions. A PAR intensity increase from 690 μmol m⁻² s⁻¹ to 1150 μmol m⁻² s⁻¹ enhanced heat tolerance of chufa plant communities, irrespective of the conditions of mineral nutrition under which they had been grown.     

Progress Update in Space Cell Mechano-biological Coupling

Recent progresses in 2018-2019 from space experiments onboard SJ-10 recoverable satellite and on parabolic flight were summarized, mainly focusing on cell mechano-biological coupling under microgravity. In the meantime, technical pre-research and experimental system design for the biomechanics research platform on China Space Station was carried out and updated.      

Modelling canopy photosynthesis in response to environmental conditions.

Physiological models in the plant and crop sciences provide a means of integrating different aspects of the system, in particular the interaction between plant processes and environmental factors. This paper focuses on the response of canopy photosynthesis, including adaptation, to environmental conditions. Adaptation is likely to be important when considering controlled ecological life support systems since physiological characteristics are affected by past as well as current environmental conditions. In particular, the level of photosynthetic enzymes in a plant is generally greater for plants grown in high irradiance levels than for similar plants grown in low irradiance. The models have been developed to apply to 'normal' growing conditions, although the principles will apply to closed bioregenerative systems.     

Potato growth in a porous tube water and nutrient delivery system.

Potato (Solanum tuberosum L.) cv. 'Norland', vegetative growth and tuber productivity grown in the porous water and nutrient delivery system (PTNDS) developed by the Wisconsin Center for Space Automation and Robotics were compared with the vegetative growth and tuber productivity of plants grown in a peat:vermiculite potting mixture (PT/VR). The plants were grown at 12, 16, and 24-h light periods, 18 degrees C constant temperature, 70% relative humidity, and 300 micromol m-2 s-1 photosynthetic photon flux. Canopy height of plants grown in the PT/VR system was taller than that of plants grown in the PTNDS system. Canopy height differences were greatest when the plants were grown under a 24-h photoperiod. Leaf and stem dry masses were similar for plants grown in the two systems under the 12-h photoperiod. Under the 24-h photoperiod, leaf and stem dry masses of plants grown in the PT/VR system were more than 3 times those of plants grown in the PTNDS system. Tuber dry masses were similar for plants grown in the two systems under the 12-h photoperiod. Under the 24 h-photoperiod, tuber dry weights of plants grown in the PT/VR system were more than twice those of plants grown in the PTNDS system. A slightly higher harvest index (ratio of tuber weight to leaf plus stem weight) was noted for the plants grown in the PTNDS than for the plants grown in the PT/VR system. Plants grown in the PTNDS system at the 24-h photoperiod matured earlier than plants grown at this photoperiod in the PT/VR system. Vegetative growth and tuber productivity of plants grown under the 16-h photoperiod generally were intermediate to those noted for plants grown under the 12 and 24-h photoperiods. These results indicate that potato plants grown in a PTNDS system may require less plant growing volume, mature in a shorter time, and likely produce more tubers per unit area compared with plants grown in the PT/VR system. These plant characteristics are a distinct advantage for a plant growing unit of a CELSS.     

Effect of wind velocity on ethylene release from lettuce plants.

Effect of wind velocity on ethylene release rate of intact lettuce plant was investigated. Lettuce plants were grown at wind velocities of 0.1, 0.4, 0.8, and 1.4 m s-1 for 25 to 33 days and then used for ethylene measurement. When ethylene release rate of the plants grown at a wind velocity of 0.1m s-1 was measured at wind velocities of 0.2, 0.6 and 1.0m s-1 the rate was not affected by wind velocity. This result indicates that ethylene diffusion from lettuce leaf to atmosphere is not affected by boundary layer conditions. When ethylene release rate of the plants grown at wind velocities of 0.1, 0.4, 0.8 and 1.4 m s-1 was measured at the same wind velocity as growing conditions, the rate was scarcely increased by high velocity of wind. A strong wind (4.0 m s-1), which induced wounding damage in small areas of the leaves, had no measurable effect on a ethylene release of the whole plant.     

Use of planetary soils within CELSS: the plant viewpoint.

The major functions of soil relative to plant growth include retention and supply of water and minerals, provision of anchorage and support for the root, and provision of an otherwise adequate physical and chemical environment to ensure an extensive, functioning root system. The physical and chemical nature of the solid matrix constituting a soil interacts with the soil confinement configuration, the growing environment, and plant requirements to determine the soil's suitability for plant growth. A wide range of natural and manufactured terrestrial materials have proven adequate soils provided they are not chemically harmful to plants (or animals eating the plants), are suitably prepared for the specific use, and are used in a compatible confinement system. It is presumed this same rationale can be applied to planetary soils for growing plants within any controlled environment life support system (CELSS). The basic concepts of soil and soil-plant interactions are reviewed relative to using soils constituted from local planetary materials for growing plants.