Excretion of amino acids by humans during space flight

We measured the urine amino acid distribution patterns before, during and after space flight on the Space Shuttle. The urine samples were collected on two separate flights of the space shuttle. The first flight lasted 9.5 days and the second flight 15 days. Urine was collected continuously on 8 subjects for the period beginning 10 d before launch to 6 d after landing. Results: In contrast to the earlier Skylab missions where a pronounced amino aciduria was found, on shuttle the urinary amino acids showed little change with spaceflight except for a marked decrease in all of the amino acids on FD (flight day) 1 (p<0.05) and a reduction in isoleucine and valine on FD3 and FD4 (p<0.05). Conclusions: (i) Amino aciduria is not an inevitable consequence of space flight. (ii) The occurrence of amino aciduria, like muscle protein breakdown is a mission specific effect rather than part of the general human response to microgravity.     

Plasma and urine catecholamine levels in cosmonauts during long-term stay on Space Station Salyut-7.

The activity of the sympathetic adrenal system in cosmonauts exposed to a stay in space lasting for about half a year has so far been studied only by measuring catecholamine levels in plasma and urine samples taken before space flight and after landing. The device "Plasma 01", specially designed for collecting and processing venous blood from subjects during space flight on board the station Salyut-7 rendered it possible for the first time to collect and freeze samples of blood from cosmonauts in the course of a long-term 237-day space flight. A physician-cosmonaut collected samples of blood and urine from two cosmonauts over the period of days 217-219 of their stay in space. The samples were transported to Earth frozen. As indicators of the sympathetic adrenal system activity, plasma and urine concentrations of epinephrine and norepinephrine as well as urine levels of the catecholamine metabolites metanephrine, normetanephrine, and vanillylmandelic acid were determined before, during and after space flight. On days 217-219 of space flight plasma epinephrine and norepinephrine levels were slightly increased, yet not substantially different from normal. During stress situations plasma norepinephrine and epinephrine levels usually exhibit a manifold increase. On days 217-219 of space flight norepinephrine and epinephrine levels in urine were comparable with pre-flight values and the levels of their metabolites were even significantly decreased. All the parameters studied, particularly plasma norepinephrine as well as urine norepinephrine, normetanephrine, and vanillylmandelic acid, reached the highest values 8 days after landing. The results obtained suggest that, in the period of days 217-219 of the cosmonauts stay in space in the state of weightlessness, the sympathetic adrenal system is either not activated at all or there is but a slight activation induced by specific activities of the cosmonauts, whereas in the process of re-adaptation after space flight on Earth this system is considerably more markedly activated.     

Changes of human serum proteome profile during 7-day “dry” immersion

The aim of this study was to characterize changes of serum proteome profile during 7-day “dry” immersion (DI). The experiment with DI consisted of three series: control group without countermeasures (10 men), with using mechanical stimulation (6 men) and low-frequency myostimulation (5 men) as preventive means. Serum samples were fractionated using ClinProt robot (Bruker Daltonics) on magnetic beads (weak cation exchange magnetic beads—MB WCX) prior to mass-spectral profiling. It was obtained 170 peaks after fractionation of serum samples in each group. On 7th immersion day peak areas of fibrinopeptide A (m/z=1206; 1464), angiotensin II (m/z=1051), high molecular mass kininogen fragment (m/z=2133 Da) and C3-fragment of the complement system (m/z=1350 Da) were significantly decreased comparing with pre-experimental values of all experimental series. Peak areas of apolipoprotein C III (m/z=9419) and C4a fragment of the complement system (m/z=3206 Da) were increased. On 7th day of the recovery peak areas of all changed peaks were not close to pre-experimental values. This fact provided evidence of incomplete recovery of an organism after DI. The depth of the alterations had considerable individual variability. Thereby the detected changes of serum proteome profile in the experiment. They indicated a reorganization of the hormonal, immune systems and lipid metabolism. The use of myostimulation and mechanical stimulation as countermeasures partly compensated adverse effects of 7-day dry immersion on the parameters of coagulation system (fibrinopeptide A) and lipid metabolism (apolipoprotein CIII).     

Blood volume regulating hormones, fluid and electrolyte modifications during 21 and 198-day space flights (Altair-MIR 1993)

During the Altair MIR' 93 mission we studied several parameters involved in blood volume regulation. The experiment was done on two cosmonauts before (B-60, B-30), during (D6, D12, D18 for French and D7, D12, D17 for Russian) and after the flight (R+1, R+3 and R+7). Space flight durations were different for two cosmonauts: for the Russian the flight duration was 198 days and for the French 21 days. On board the MIR station only urinary (volume and electrolytes, atrial natriuretic peptide (ANP), cyclic guanosine monophosphate (cGMP) and catecholamines) and salivary (cGMP and cortisol) samples were collected, centrifuged and stored in freezer. Lithium was used as a tracer to know exactly the 24 h urine output (CNES urine collection Kit). Before and after flight, blood was drawn with an epicite needle and vacutainer system for hormonal assays (renin, antidiuretic hormone, cGMP, ANP and aldosterone) in two positions: after 30 min rest in upright seated position and after 90 min of supine position. Salivary samples were collected simultaneously. During flight a decrease of diuresis and ANP and an increase of osmolality were found. No modifications of hematocrit, but an increase of salivary cGMP and cortisol were also observed. The decrease of urinary ANP is in favor of hypovolemia as described in previous flights. The postflight examinations revealed changes in fluid-electrolyte metabolism which indicate a hypohydration status and a stimulation of hormonal system responsible for water and electrolyte retention in order to readapt to the normal gravity.     

Mutagenesis in bacterial spores exposed to space and simulated martian conditions: data from the EXPOSE-E spaceflight experiment PROTECT

As part of the PROTECT experiment of the EXPOSE-E mission on board the International Space Station (ISS), the mutagenic efficiency of space was studied in spores of Bacillus subtilis 168. After 1.5 years' exposure to selected parameters of outer space or simulated martian conditions, the rates of induced mutations to rifampicin resistance (Rif(R)) and sporulation deficiency (Spo(-)) were quantified. In all flight samples, both mutations, Rif(R) and Spo(-), were induced and their rates increased by several orders of magnitude. Extraterrestrial solar UV radiation (>110?nm) as well as simulated martian UV radiation (>200?nm) led to the most pronounced increase (up to nearly 4 orders of magnitude); however, mutations were also induced in flight samples shielded from insolation, which were exposed to the same conditions except solar irradiation. Nucleotide sequencing located the Rif(R) mutations in the rpoB gene encoding the β-subunit of RNA polymerase. Mutations isolated from flight and parallel mission ground reference (MGR) samples were exclusively localized to Cluster I. The 21 Rif(R) mutations isolated from the flight experiment showed all a C to T transition and were all localized to one hotspot: H482Y. In mutants isolated from the MGR, the spectrum was wider with predicted amino acid changes at residues Q469K/L/R, H482D/P/R/Y, and S487L. The data show the unique mutagenic power of space and martian surface conditions as a consequence of DNA injuries induced by solar UV radiation and space vacuum or the low pressure of Mars.     

Trajectories for missions of low-thrust spacecraft aimed at delivery of soil samples from main belt asteroids and Phobos

Trajectories of spacecraft with electro-jet low-thrust engines are studied for missions planning to deliver samples of matter from small bodies of the Solar System: asteroids Vesta and Fortuna, and Martian moon Phobos. Flight trajectories are analyzed for the mission to Phobos, the limits of optimization of payload spacecraft mass delivered to it are determined, and an estimate is given to losses in the payload mass when a low-thrust engine with constant outflow velocity is used. The model of an engine with ideally regulated low thrust is demonstrated to be convenient for calculations and analysis of flight trajectories of a low-thrust spacecraft.     

Conceptual study and key technology development for Mars Aeroflyby sample collection

Conceptual study of Mars Aeroflyby Sample Collection (MASC) is conducted as a part of the next Mars exploration mission currently entertained in Japan Aerospace Exploration Agency. In the mission scenario, an atmospheric entry vehicle is flown into the Martian atmosphere, collects the Martian dust particles as well as atmospheric gases during the guided hypersonic flight, exits the Martian atmosphere, and is inserted into a parking orbit from which a return system departs for the earth to deliver the dust and gas samples. In order to accomplish a controlled flight and a successful orbit insertion, aeroassist orbit transfer technologies are introduced into the guidance and control system. System analysis is conducted to assess the feasibility and to make a conceptual design, finding that the MASC system is feasible at the minimum system mass of 600 kg approximately. The aerogel, which is one of the candidates for the dust sample collector, is assessed by arcjet heating tests to examine its behavior when exposed to high-temperature gases, as well as by particle impingement tests to evaluate its dust capturing capability.     

Space motion sickness medications: interference with biomedical parameters

The possibility that drugs administered to Skylab 3 (SL-3) and 4 (SL-4) crewmen for space motion sickness may have interfered with their biomedical evaluation in space was investigated. Healthy volunteers received combinations of Scopolamine/Dexedrine for four days in regimens similar to those used in these missions. Urine samples, heart rate, body temperature, mood and performance were analyzed for drug-related changes. Twenty-four hour urine samples were analyzed by the same procedures as those used to analyze the flight samples. Hormone concentrations determined included cortisol, epinephrine, norepinephrine, aldosterone and antidiuretic hormone (ADH). In addition, volume, specific gravity, osmolarity, sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), chloride (Cl), inorganic phosphate, uric acid and creatinine were measured. Performance was not affected by the Scopolamine/Dexedrine. The drug combination increased daily mean heart rate (HR) significantly in all the subjects and daily mean rectal temperature (RT) in some of the subjects. A 2-4 hr phase shift in the HR circadian rhythm was also observed which indicates that internal circadian synchrony was disturbed by the drugs. Psychological and subjective evaluation indicated that the subjects could usually identify which days they were given the drugs by an increase in tension and anxiety, decreased patience, restlessness, decreased appetite, difficulty in sleeping and feelings of increased heart rate and body temperature. Urinary electrolytes were not changed significantly by the drug, but marked and significant changes occurred in urine volume and hormone excretion patterns. Scopolamine/Dexedrine caused consistent elevations in urinary cortisol and epinephrine and a transient elevation in ADH. Norepinephrine excretion was decreased, but there was no significant change in aldosterone excretion or in 24 hr urine volume. A comparison of these findings with the first four days of inflight data from the SL-3 and SL-4 missions leads to the conclusion that the dramatic increases in aldosterone excretion during the first three days of spaceflight probably can be directly attributed to weightlessness, whereas the antimotion sickness medication could have substantially contributed to the early increased excretion of epinephrine and cortisol during these missions.     

Endocrine responses in long-duration manned space flight

The bioassay of body fluids experiment is designed to evaluate the biochemical adaptation resulting from extended exposure to space flight environment by identifying changes in hormonal and associated fluid and electrolyte parameters reflected in the blood and urine of the participating crewmen. The combined stresses of space flight include weightlessness, acceleration, confinement, restraint, long-term maintenance of high levels of performance, and possible desynchronosis. Endocrine measurements to assess the physiological cost of these stresses have been considered from two aspects. Fluid and electrolyte balance have been correlated with weight loss, changes in the excretion of aldosterone and vasopressin and fluid compartments. The second area involves the estimation of the physiological cost of maintaining a given level of performance during space flight by analysis of urinary catecholamines and cortisol. Inter-individual variability was demonstrated in most experimental indices measured; however, constant patterns have emerged which include: body weight change; increases in plasma renin activity; elevations in urinary catecholamines, ADH, aldosterone and cortisol concentrations. Plasma cortisol decreases in immediate postflight samples with subsequent increase in 24-hour urines. The measured changes are consistent with the prediction that a relative increase in thoracic blood volume upon transition to the zero-gravity environment is interpreted as a true volume expansion resulting in an osmotic diuresis. This diuresis in association with other factors ultimately results in a reduction in intravascular volume, leading to an increase in renin and a secondary aldosteronism. Once these compensatory mechanisms are effective in reestablishing positive water balance, the crewmen are considered to be essentially adapted to the null-gravity environment. Although the physiological cost of this adaptation must reflect the electrolyte deficit and perhaps other factors, it is assumed that the compensated state is adequate for the demands of the environment; however, this new homeostatic set is not believed to be without physiological cost and could, except with proper precautions, reduce the functional reserve of exposed individuals.     

Ares I–X Flight Test Vehicle similitude to the Ares I Crew Launch Vehicle

The Ares I–X Flight Test Vehicle is the first in a series of flight test vehicles that will take the Ares I Crew Launch Vehicle design from development to operational capability. Ares I–X is scheduled for a 2009 flight date, early enough in the Ares I design and development process so that data obtained from the flight can impact the design of Ares I before its Critical Design Review. Decisions on Ares I–X scope, flight test objectives, and FTV fidelity were made prior to the Ares I systems requirements being baselined. This was necessary in order to achieve a development flight test to impact the Ares I design. Differences between the Ares I–X and the Ares I configurations are artifacts of formulating this experimental project at an early stage and the natural maturation of the Ares I design process. This paper describes the similarities and differences between the Ares I–X Flight Test Vehicle and the Ares I Crew Launch Vehicle. Areas of comparison include the outer mold line geometry, aerosciences, trajectory, structural modes, flight control architecture, separation sequence, and relevant element differences. Most of the outer mold line differences present between Ares I and Ares I–X are minor and will not have a significant effect on overall vehicle performance. The most significant impacts are related to the geometric differences in Orion Crew Exploration Vehicle at the forward end of the stack. These physical differences will cause differences in the flow physics in these areas. Even with these differences, the Ares I–X flight test is poised to meet all five primary objectives and six secondary objectives. Knowledge of what the Ares I–X flight test will provide in similitude to Ares I—as well as what the test will not provide—is important in the continued execution of the Ares I–X mission leading to its flight and the continued design and development of Ares I.     

Estimation of dynamic characteristics of the <Emphasis Type="Italic">International Space Station</Emphasis> from measurements of microaccelerations

We describe the results of determining the mass of the International Space Station using the data of MAMS accelerometer taken during correction of the station orbit on August 20, 2004. The correction was made by approach and attitude control engines (ACE) of the Progress transporting spacecraft. The engines were preliminary calibrated in an autonomous flight using the onboard device for measuring apparent velocity increment. The method of calibration is described and its results are presented. The error in station mass determination is about 1%. The same data of MAMS and similar data obtained during the orbit correction on August 26, 2004 were used for the analysis of high-frequency vibrations of the station mainframe caused by operation of the ACE of Progress. Natural frequencies of the ACE are determined. They lie in the frequency band 0.024–0.11 Hz. ACE operation is demonstrated to result in a substantial increase of microaccelerations onboard the station in the frequency range 0–1 Hz. The frequencies are indicated at which disturbances increase by more than an order of magnitude. The study described was carried out as a part of the Tensor technological experiment.     

以RBCC为动力的巡航飞行器有效载荷质量敏感性分析

基于飞行轨迹及质量分析数学模型,对以RBCC为动力的巡航飞行器有效载荷的敏感性进行了分析,主要考虑了发动机比冲、发射马赫数、发射高度、模态转换点(转换马赫数)及惰性质量系数等对有效载荷质量的影响。分析结果表明,提高发射马赫数和发射高度、增加发动机比冲、降低模态转换马赫数及飞行器惰性质量系数有利于提高巡航飞行器的有效载荷质量。其中有效载荷质量对惰性质量系数最敏感,当惰性质量系数分别减小7.3%和增大7.3%时,有效载荷质量的增大量和减小量将分别达到58%和103.7%。     

随动推力作用下柔性旋转飞行器稳定性分析

针对柔性旋转飞行器动力学问题,考虑了飞行器转速的作用,建立了计入陀螺力矩及随动推力影响的运动方程并分析了系统的动力稳定性问题。将柔性旋转飞行器简化为带有附加质量的非均匀转子结构,考虑陀螺力矩及随动推力的影响,采用剪切变形对轴向位移有影响的Timoshenko梁模型,基于有限元方法建立了运动方程,分析转速、剪切刚度及附加质量等因素对系统稳定性的影响,发现了转速作用下旋转飞行器刚体和弹性体模态耦合的新现象。结果表明：剪切刚度较小时,剪切变形对临界推力有较大影响;附加质量的大小及位置对临界推力和不稳定域有重要的影响;转速一般会诱发非均匀转子系统的弯曲模态与刚体模态合并为一个耦合模态,致使系统产生动态失稳。     

A study of metabolic balance in crewmembers of Skylab IV

A metabolic balance study was conducted on the three crewmembers of the 84-day Skylab IV earth orbital mission. Dietary intake was controlled, monitored, and kept very nearly constant for a period commencing 21 days prior to flight, throughout flight, and for a period of 18 days postflight. Within the first 30 days of flight urine calcium rose to a level approx. 100% above preflight levels and remained elevated for the remainder of the flight. Fecal calcium excretion increased more slowly but continued to accelerate throughout the flight and did not return to baseline levels during the postflight period. Urinary nitrogen increased to 25-30% above preflight levels within one month following launch and thereafter gradually subsided toward control values. The overall losses of calcium averaged approx. 200 mg per day throughout the mission while nitrogen losses averaged 590 mg. Various other indices of musculoskeletal deterioration are discussed and correlated. The parallelism between the effects of weightlessness and bed rest is reviewed. It is noted, that no evidence is yet available as to the identity of the initial biological response to the absence of gravity.     

MPT在探月中的应用方案初探

借鉴“东方红3号”卫星的推进系统组成和“SMART-1”探月器的飞行轨道,提出了2种微波等离子体推力器(MPT)应用于月球探测器的推进系统方案(即复合推进方案和统一推进方案),分析计算了MPT用于姿态控制时对推进剂耗量的影响及用于主推进变轨时推进剂耗量和飞行时间的变化,并讨论了MPT的电源系统带来的附加质量。结果表明,在付出一定飞行时间代价的条件下,MPT的引入将大大增加有效载荷质量。     

Disturbance in the lower ionosphere that accompanied the reentry of the Chelyabinsk cosmic body

The paper describes quasi-periodic and aperiodic variations in the phase and amplitude of radio waves of LF and VLF ranges, which accompanied the flight and explosion of the Chelyabinsk meteoroid. Quasi-periodic variations in the phase have been explained by the generation of acoustic-gravity waves in the atmosphere, which modulate the electron density in the ionosphere and the phase of radio waves. Aperiodic variations in the phase and amplitude of radio waves are associated with an increase in the electron density in the lower ionosphere (at altitudes of 65–70 km). This increase was most likely caused by the interactions of subsystems in the Earth–atmosphere–ionosphere–magnetosphere system or, more correctly, by the precipitation of high-energy electrons from the magnetosphere into the lower ionosphere, which was stimulated by the flight and explosion of a cosmic body. According to the estimates, the density of the flux of electrons with energies of 100 KeV should be on the order of 106 m^–2 s^–1.     

Metabolic changes in the animals subjected to space flight

The activity of the enzymes involved in aminoacid metabolism (tyrosine aminotransferase, TAT, tryptophan pyrrolase TP, serine dehydratase, SD) with rapid response to glucocorticoids and enzymes requiring for activity increase repeated administration of corticosterone (alanine aminotransferase, ALT, aspartate aminotransferase, AST) in liver, the changes of lipolysis in adipose tissue and the plasma corticosterone levels were studied in rats subjected to space flight (F), in animals from synchron model experiments (SM, simulated conditions of space flight in laboratory) and in intact controls (C). The increase of plasma corticosterone concentration and of the activity of rapidly (TAT, TP, SD) and slowly activating enzymes (ALT, AST) was found in F group 6-10 hr after space flight (18.5 days on biosatellite COSMOS 1129). This suggested the presence of acute-stress (associated primarily with the landing) and chronic stress induced hypercorticosteronemia during the flight. After the short 6-day period of recovery the plasma corticosterone concentrations and the activities of liver enzymes returned to control levels. The exposition of animals to repeated immobilization stress showed higher response of corticosterone levels in flight rats as compared to intact controls. No changes in basal lipolysis were observed in flight rats in comparison to intact controls, however the stimulation of lipolysis by norepinephrine was lower in animals from F and SM groups. This lower response of lipolytic processes to norepinephrine was found in flight animals also after six days period of recovery. These results showed that there are important changes in the regulation of lipolytic processes in adipose tissue of rats after space flight and in the conditions of model experiments.     

Reloading partly recovers bone mineral density and mechanical properties in hind limb unloaded rats

Skeletal unloading results in decreased bone formation and bone mass. During long-term space flight, the decreased bone mass is impossible to fully recover. Therefore, it is necessary to develop the effective countermeasures to prevent spaceflight-induced bone loss. Hindlimb Unloading (HLU) simulates effects of weightlessness and is utilized extensively to examine the response of musculoskeletal systems to certain aspects of space flight. The purpose of this study is to investigate the effects of a 4-week HLU in rats and subsequent reloading on the bone mineral density (BMD) and mechanical properties of load-bearing bones.     

Role of sample return and sample science in low cost missions

Sample return is an essential component of solar system exploration. Samples provide a unique data set that is critical for understanding formation and evolution of our solar system. This uniqueness is based on the scale of observations, precision of measurements, the ability to modify experiments as logic and technology dictate, and the ability to use instruments free of the constraints on mass, power, reliability, and data rate of flight instruments. Advances in analytical capabilities in recent years enable fundamental measurements to be made on extremely small samples, greatly reducing mass constraints on robotic sample return spacecraft. Sample studies provide irreplaceable ground truth for remotely-sensed data on planetary surfaces and fit within a variety of architectures for human exploration of the solar system.