Gravitropism of basidiomycetous fungi--on Earth and in microgravity.

In order to achieve perfect positioning of their lamellae for spore dispersal, fruiting bodies of higher fungi rely on the omnipresent force gravity. Only accurate negatively gravitropic orientation of the fruiting body cap will guarantee successful reproduction. A spaceflight experiment during the STS-55 Spacelab mission in 1993 confirmed that the factor gravity is employed for spatial orientation. Most likely every hypha in the transition zone between the stipe and the cap region is capable of sensing gravity. Sensing presumably involves slight sedimentation of nuclei which subsequently causes deformation of the net-like arrangement of F-actin filament strands. Hyphal elongation is probably driven by hormone-controlled activation and redistribution of vesicle traffic and vesicle incorporation into the vacuoles and cell walls to subsequently cause increased water uptake and turgor pressure. Stipe bending is achieved by way of differential growth of the flanks of the upper-most stipe region. After reorientation to a horizontal position, elongation of the upper flank hyphae decreases 40% while elongation of the lower flank slightly increases. On the cellular level gravity-stimulated vesicle accumulation was observed in hyphae of the lower flank.     

Graviresponses in fungi.

Although the orientation of mycelial hyphal growth is usually independent of the gravity vector, individual specialised hyphae can show response to gravity. This is exemplified by the sporangiophore of Phycomyces, but the most striking gravitropic reactions occur in mushroom fruit bodies. During the course of development of a mushroom different tropisms predominate at different times; the young fruit body primordium is positively phototropic, but negative gravitropism later predominates. The switch between tropisms has been associated with meiosis. The spore-bearing tissue is positively gravitropic and responds independently of the stem. Bracket polypores do not show tropisms but exhibit gravimorphogenetic responses: disturbance leads to renewal of growth producing an entirely new fruiting structure. Indications from both clinostat and space flown experiments are that the basic form of the mushroom (overall tissue arrangement of stem, cap, gills, hymenium, veil) is established independently of the gravity vector although maturation, and especially commitment to the meiosis-sporulation pathway, requires the normal gravity vector. The gravity perception mechanism is difficult to identify. The latest results suggest that disturbance of cytoskeletal microfilaments is involved in perception (with nuclei possibly being used as statoliths), and Ca2(+)-mediated signal transduction may be involved in directing growth differentials.     

Morphometric analysis of cell size patterning involved in gravitropic curvature of the stipe of Coprinus cinereus.

During gravitropic bending of the stipe of Coprinus cinereus the majority of elongation occurred in the apical region of the lower surface of the stipe, although some elongation was seen throughout the stipe. The final rate of elongation was similar at both the upper and lower stipe surfaces but the lower surface achieved this rate first (close to the reaction time 25 min), whilst the upper surface of the stipe only attained its final elongation rate after a period of acceleration of 150 min. Detailed morphometric analysis of cell size patterning in transverse sections revealed no significant differences in cross sectional area, spatial or proportional distribution of different cell types between the upper and lower regions of the gravitropic bend. Measurements of longitudinal cell size revealed significant differences in compartment size between the lower and upper region. Hyphal compartments of lower regions of the bend were on average four to five times longer than those of the upper region.     

Differentiation of Dictyostelium discoideum vegetative cells into spores during Earth orbit in space.

We reported previously that emerged amoebae of Dictyostelium (D.) discoideum grew, aggregated and differentiated to fruiting bodies with normal morphology in space. Here, we investigated the effects of space radiation and/or microgravity on the number, viability, kinetics of germination, growth rate and mutation frequency of spores formed in space in a radiation-sensitive strain, gamma s13, and the parental strain, NC4. In gamma s13, there were hardly spores in the fruiting bodies formed in space. In NC4, we found a decrease in the number of spores, a delay in germination of the spores and delayed start of cell growth of the spores formed in space when compared to the ground control. However, the mutation frequency of the NC4 spores formed in space was similar to that of the ground control. We conclude that the depression of spore formation might be induced by microgravity and/or space radiation through the depression of some stage(s) of DNA repair during cell differentiation in the slime mold.     

Structure of cress root statocytes in microgravity (Bion-10 mission).

Experiments on primary roots of Lepidium sativum L. have been performed on board the Bion-10 satellite. The experimental set-up was extremely miniaturized and completely automatic. The results demonstrate the effectiveness of the instrumentation. The spatial orientation, growth, root cap differentiation and statocyte structure of roots grown under microgravity (MG) have been compared with control roots grown on the ground (GC) and in a 1G-reference centrifuge in space (RC). Root length and cap shape did not differ between MG and control samples. Under MG, the mean distance of the statoliths from the distal cell wall of the statocytes increased significantly, the mean distance of the mitochondria decreased and the nucleus did not change its position in comparison to both controls. The number and the shape of the amyloplasts (statoliths) were not influenced by the space flight factors, but their size as well as their relative area in the cell decreased. The number of starch grains per statolith as well as their size and shape changed under MG. In MG and RC samples the number of lipid bodies in the statocytes was higher and the relative area larger than in GC samples. The relative area occupied by vacuoles was greater in RC statocytes than in GC and MG statocytes. These results partly confirm and, in addition, extend the data from earlier experiments in space.     

Attitude analysis of space debris using SLR and light curve data measured with single-photon detector

Attitude is the important parameter for active debris removal and collision avoidance. This paper deduced the spin axis orientation and spin period of the rocket body, CZ-3B R/B (NORAD ID 38253), using the satellite laser ranging and light curve data measured with single-photon detector at Graz station. The epoch method and LC & SLR residuals fitting were combined to determine these values. The derived right ascension angle was around 220°, the declination angle was near 64° and the sidereal period was calculated to be 117.724 s, for 2017-07-03. The results derived from the two distinct methods were mutually validated. Rocket bodies are a major contributor to space debris and this work provides a reference for attitude determination and attitude modelling.     

Gravisensing: ionic responses, cytoskeleton and amyloplast behavior.

In Zea mays L., changes in orientation of stems are perceived by the pulvinal tissue, which responds to the stimulus by differential growth resulting in upward bending of the stem. Gravity is perceived in the bundle sheath cells, which contain amyloplasts that sediment to the new cell base when a change in the gravity vector occurs. The mechanism by which the mechanical signal is transduced into a physiological response is so far unknown for any gravity perceiving tissue. It is hypothesized that this involves interactions of amyloplasts with the plasma membrane and/or ER via cytoskeletal elements. To gain further insights into this process we monitored amyloplast movements in response to gravistimulation. In a pharmacological approach we investigated how the dynamics of plastid sedimentation are affected by actin and microtubule (MT) disrupting drugs. Dark grown caulonemal filaments of the moss Physcomitrella patens respond to gravity vector changes with a reorientation of tip growth away from the gravity vector. MT distributions in tip cells were monitored over time and MTs were seen to accumulate preferentially on the lower flank of the tip 30 min after a 90 degree turn. Using a self-referencing Ca2+ selective ion probe, we found that growing caulonemal filaments exhibit a Ca2+ influx at the apical dome, similar to that reported previously for other tip growing cells. However, in gravistimulated Physcomitrella filaments the region of Ca2+ influx is not confined to the apex, but extends about 60 micrometers along the upper side of the filament. Our results indicate an asymmetry in the Ca2+ flux pattern between the upper and side of the filament suggesting differential activation of Ca2+ permeable channels at the plasma membrane.     

Gravitaxis and graviperception in Euglena gracilis.

Gravitactic orientation in the flagellate Euglena gracilis is mediated by an active physiological receptor rather than a passive alignment of the cells. During a recent space flight on the American shuttle Columbia the cells were subjected to different accelerations between 0 and 1.5 x g and tracked by computerized real-time image analysis. The dependence of orientation on acceleration followed a sigmoidal curve with a threshold at < or = 0.16 x g and a saturation at about 0.32 x g. No adaptation of the cells to the conditions of weightlessness was observed over the duration of the space mission (12 days). Under terrestrial conditions graviorientation was eliminated when the cells were suspended in a medium the density of which (Ficoll) equaled that of the cell body (1.04 g/ml) and was reversed at higher densities indicating that the whole cytoplasm exerts a pressure on the respective lower membrane. There it probably activates stretch-sensitive calcium specific ion channels since gravitaxis can be affected by gadolinium which is a specific inhibitor of calcium transport in these structures. The sensory transduction chain could involve modulation of the membrane potential since ion channel blockers, ionophores and ATPase inhibitors impair graviperception.     

Gravity effects on the [correction of thr] growth and development of moss secondary protonemata.

The superficial cells of dark-grown moss shoots give rise to negatively gravitropic protonemata, whatever the orientation of the shoot. Shoot orientation, however, does affect from which side of the shoot the protonemata form and the direction of their growth. Protonemata from horizontal shoots grow out at a near-right angle to their supporting axes and are initiated more or less evenly along the upper side of the stem. Protonemata arising from vertically-oriented shoots in either an upright or an inverted position grow straight at an acute angle to the stem axis. The difference in the growth direction of the protonemata seems to be conditioned by the different position of the growth zone of the protonemal outgrowths, and subsequently that of the apical protonemal cells, with respect to the gravity vector. Observations suggest that the shoot protonemata, in conditions of clinorotation, persist in their original growth direction. Results also indicate that, in darkness, gravity determines only the site of protonemata initiation, not the process of initiation itself. Light, by contrast, by acting through both phytochrome and high-energy reaction systems, triggers the initiation process and defines the location of protonemata.     

空间飞行体与压缩区内等离子体非稳态相互作用的数值模拟

在压缩区内，飞行体天线辐射出的高频调制场影响着等离子体中的电荷分布，而电荷分布的扰动又反过来影响场量，采用FTCS有限差分格式方法，利用二维三分量轴对称，对空间飞行体与压缩区内等离子体非稳态相互作用过程进行数值模拟，得到了电荷密度扰动与电场的变化情况，计算结果表明，这种非线性相互作用可以引起场的塌缩并出现密度空洞，通过对密度空洞变化的探测，可对隐身飞行体进行跟踪。     

低高度内辐射带高能质子空间分布位形的研究

利用国际地磁参考场模式(IGRF模式)分析了1970-2000年低高度南大西洋负磁异常区位形的漂移与变化，给出了几个高度异常区中心位置磁场强度的变化和位置的变化。利用带电粒子的运动学方程，简要分析了低高度辐射带高能粒子的运动，得出在低高度，磁场是决定辐射带高能粒子空间强度与分布的决定性因素．低高度辐射带空间分布位形的变化特征应该与低高度南大西洋负磁异常区的变化特征基本一致．低高度南大西洋负磁异常区的特征可以作为低高度辐射带空间分布位形的一个初步判据。     

Effects of space flight and IGF-1 on immune function

We tested the hypothesis that insulin-like growth factor-1 (IGF-1) would ameliorate space flight-induced effects on the immune system. Twelve male, Sprague-Dawley rats, surgically implanted with mini osmotic pumps, were subjected to space flight for 10 days on STS-77. Six rats received 10 mg/kg/day of IGF-1 and 6 rats received saline. Flight animals had a lymphocytopenia and granulocytosis which were reversed by IGF-1. Flight animals had significantly higher corticosterone levels than ground controls but IGF-1 did not impact this stress hormone. Therefore, the reversed granulocytosis did not correlate with serum corticosterone. Space flight and IGF-1 also combined to induce a monocytopenia that was not evident in ground control animals treated with IGF-1 or in animals subjected to space flight but given physiological saline. There was a significant increase in spleen weights in vivarium animals treated with IGF-1, however, this change did not occur in flight animals. We observed reduced agonist-induced lymph node cell proliferation by cells from flight animals compared to ground controls. The reduced proliferation was not augmented by IGF-1 treatment. There was enhanced secretion of TNF, IL-6 and NO by flight-animal peritoneal macrophages compared to vivarium controls, however, O₂ secretion was not affected. These data suggest that IGF-1 can ameliorate some of the effects of space flight but that space flight can also impact the normal response to IGF-1.     

A deformation model of flexible,HAMR objects for accurate propagation under perturbations and the self-shadowing effects

A new type of space debris in near geosynchronous orbit (GEO) was recently discovered and later identified as exhibiting unique characteristics associated with high area-to-mass ratio (HAMR) objects, such as high rotation rates and high reflection properties. Observations have shown that this debris type is very sensitive to environmental disturbances, particularly solar radiation pressure, due to the fact that its motion depends on the actual effective area, orientation of that effective area, reflection properties and the area-to-mass ratio of the object is not stable over time. Previous investigations have modelled this type of debris as rigid bodies (constant area-to-mass ratios) or discrete deformed body; however, these simplifications will lead to inaccurate long term orbital predictions. This paper proposes a simple yet reliable model of a thin, deformable membrane based on multibody dynamics. The membrane is modelled as a series of flat plates, connected through joints, representing the flexibility of the membrane itself. The mass of the membrane, albeit low, is taken into account through lump masses at the joints. The attitude and orbital motion of this flexible membrane model is then propagated near GEO to predict its orbital evolution under the perturbations of solar radiation pressure, Earth’s gravity field (J₂), third body gravitational fields (the Sun and Moon) and self-shadowing. These results are then compared to those obtained for two rigid body models (cannonball and flat rigid plate). In addition, Monte Carlo simulations of the flexible model by varying initial attitude and deformation angle (different shape) are investigated and compared with the two rigid models (cannonball and flat rigid plate) over a period of 100?days. The numerical results demonstrate that cannonball and rigid flat plate are not appropriate to capture the true dynamical evolution of these objects, at the cost of increased computational time.     

Multi-Sphere Method for modeling spacecraft electrostatic forces and torques

The use of electrostatic (Coulomb) actuation for formation flying is attractive because non-renewable fuel reserves are not depleted and plume impingement issues are avoided. Prior analytical electrostatic force models used for Coulomb formations assume spherical spacecraft shapes, which include mutual capacitance and induced effects. However, this framework does not capture any orientation-dependent forces or torques on generic spacecraft geometries encountered during very close operations and docking scenarios. The Multi-Sphere Method (MSM) uses a collection of finite spheres to represent a complex shape and analytically approximate the Coulomb interaction with other charged bodies. Finite element analysis software is used as a truth model to determine the optimal sphere locations and radii. The model is robust to varying system parameters such as prescribed voltages and external shape size. Using the MSM, faster-than-realtime electrostatic simulation of six degree of freedom relative spacecraft motion is feasible, which is crucial for the development of robust relative position and orientation control algorithms in local space situational awareness applications. To demonstrate this ability, the rotation of a cylindrical craft in deep space is simulated, while charge control from a neighboring spacecraft is used to de-spin the object. Using a 1 m diameter craft separated by 10 m from a 3 by 1 m cylindrical craft in deep space, a 2 °/s initial rotation rate can be removed from the cylinder within 3 days, using electric potentials up to 30 kV.     

转盘轴承集聚效应分析及其对回转性能的影响

针对航天发射台转盘轴承工作时出现滚动体集聚导致摩擦力矩增大的问题,研究转盘轴承中滚动体之间间隙变化对集聚效应的影响。通过对典型工位处进行有限元分析,对比不同接触类型转盘轴承的滚道变形和滚动体接触作用力的分布规律。在确定滚动体与隔离块的几何关系后,进一步分析了滚道变形和滚动体接触作用力的时变性对滚动体间隙变化的影响,并将间隙变化和集聚效应建立了联系。有限元计算得到的发射台框架变形与实验测量数据一致,验证了有限元计算的可靠性。仿真结果显示,由于下滚道发生轴向变形,滚动体在运动过程中不断交替进行“爬坡”和“下坡”;滚道径向变形导致滚动体之间增大了较多的间隙;各滚动体的驱动摩擦力不断变化,运动状态具有时变性,当转盘轴承中间隙增加时,运动状态不断变化的滚动体导致增加的间隙出现累积,引起滚动体集聚。为消除滚动体集聚效应,探索其对摩擦力矩的影响,进行了对比实验。结果表明:集聚效应导致发射台的摩擦力矩增加,且变化剧烈。      

前体非对称涡Re效应初探及其风洞模拟技术

对不同长细比(11和6.15)的细长旋成体模型在低速风洞中完成了亚临界和 临界雷诺数(Re)的测压实验研究.结果表明,只要后体尾部截断至离二涡区足够远,就不会影响由前体二涡主控的多涡系结构,并且头部扰动与非对称涡响应之间的相关关系也保持不变,这为在常规低速风洞中通过增大旋成体直径、减小长细比来扩大Re实验范围提供了实验依据.基于此技术,临界Re下低速实验结果表明细长旋成体在层流和转捩分离区的截面压力分布有明显的区别,导致在临界Re内的侧向力较亚临界显著减小,而且头部扰动对背涡流动的主控作用明显减弱,单孔位微吹气扰动主动控制技术不再适用.      

Effects of rectilinear acceleration, caloric and optokinetic stimulation of human subjects in the Spacelab D-1 mission

A set of vestibular experiments was performed during the course of the German Spacelab D-1 mission from 30 October to 6 November 1985 by a consortium of experimenters from various european countries. Similar to the Spacelab SL-1 mission all of the scientific crew members were theoretically and practically trained for the experiments. Baseline measurements for all tests were collected 113, 86, 44, 30 and 18 days prior to the mission and compared with data taken inflight, on the landing day and the consecutive 7 to 14 days.

The hardware comprised mainly a motordriven accelerating platform, the SPACE SLED, and the vestibular helmet, a multi-purpose instrument in support of a variety of vestibular experiments including air-calorisation of the ears, optokinetic stimulation pattern presentation and optical and nystagmographic recording of eye movements.

Measurements of the threshold for the perception of detection of whole body movement did not reveal any dramatic changes in the 2 measured axes inflight when compared to preflight values. Early postflight values show a significantly elevated threshold for all axes in 3 out of 4 subjects.

The caloric nystagmus, already found during the SL-1 mission, was confirmed on all three tested subjects during the D-1 mission. It's amplitude and in some instances it's direction were influenced by horizontal acceleration on the SLED.

The amplitude of optokinetic nystagmus increased when subjects were allowed to free-float over that seen when subjects were fixed.

Stimulation of the neck receptors by roll movements of the body against the fixated head resulted in illusory object motion to the contralateral side. Torsional movements of the eyes during such neck receptor stimulation was present inflight and postflight, while it had not been observed preflight. Most results point to a reduction of otolithic effects in favour of visual and proprioceptive influences for spatial orientation.     

基于人体模型的舱外航天服热系统分析与计算

分析了舱外航天服空间换热的特点,对"空间环境-舱外航天服-人体"热系统进行了传热分析,利用Visual C++ 6.0及OpenGL技术进行了三维人体模型的构造,对人体体表温度的计算结果进行了三维图形显示.结合人体热调节模型建立了"空间环境-舱外航天服-人体"热系统仿真技术,分析了航天员在不同代谢模式、被动热防护状态及液冷、通风系统控制情况下的热状态,确定了各代谢活动水平下航天员达到舒适状态时液冷、通风系统的工作条件,利用暖体假人试验结果对系统仿真进行了验证,结果表明二者吻合很好,本项目具有很好的工程应用价值.