γ-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin

Ionizing radiation (IR) exposure is inevitable in our modern society and can lead to a variety of deleterious effects including cancer and birth defects. A reliable, reproducible and sensitive assessment of exposure to IR and the individual response to that exposure would provide much needed information for the optimal treatment of each donor examined. We have developed a diagnostic test for IR exposure based on detection of the phosphorylated form of variant histone H2AX (γ-H2AX), which occurs specifically at sites of DNA double-strand breaks (DSBs). The cell responds to a nascent DSB through the phosphorylation of thousands of H2AX molecules flanking the damaged site. This highly amplified response can be visualized as a γ-H2AX focus in the chromatin that can be detected in situ with the appropriate antibody. Here we assess the usability of γ-H2AX focus formation as a possible biodosimeter for human exposure to IR using peripheral blood lymphocytes irradiated ex vivo and three-dimensional artificial models of human skin biopsies. In both systems, the tissues were exposed to 0.2–5 Gy, doses of IR that might be realistically encountered in various scenarios such as cancer radiotherapies or accidental exposure to radiation. Since the γ-H2AX response is maximal 30 min after exposure and declines over a period of hours as the cells repair the damage, we examined the time limitations of the useful detectability of γ-H2AX foci. We report that a linear response proportional to the initial radiation dose was obtained 48 and 24 h after exposure in blood samples and skin cells respectively. Thus, detection of γ-H2AX formation to monitor DNA damage in minimally invasive blood and skin tests could be useful tools to determine radiation dose exposure and analyze its effects on humans.     

The optical emission lines of type 1 X-ray bright Active Galactic Nuclei

A strong X-ray emission is one of the defining signatures of nuclear activity in galaxies. According to the Unified Model for Active Galactic Nuclei (AGN), both the X-ray radiation and the prominent broad emission lines, characterizing the optical and UV spectra of Type 1 AGNs, are originated in the innermost regions of the sources, close to the Super Massive Black Holes (SMBH), which power the central engine. Since the emission is concentrated in a very compact region (with typical size r?0.1$r?0.1$ pc) and it is not possible to obtain resolved images of the source, spectroscopic studies of this radiation represent the only valuable key to constrain the physical properties of matter and its structure in the center of active galaxies. Based on previous studies on the physics of the Broad Line Region (BLR) and on the X-ray spectra of broad (FWHM_Hβ ? 2000 km s⁻¹) and narrow line (1000 km s⁻¹ ?FWHM_Hβ ? 2000 km s⁻¹) emitting objects, it has been observed that the kinematic and ionization properties of matter close to the SMBHs are related together, and, in particular, that ionization is higher in narrow line sources. Here we report on the study of the optical and X-ray spectra of a sample of Type 1 AGNs, selected from the Sloan Digital Sky Survey (SDSS) database, within an upper redshift limit of z=0.35$z=0.35$, and detected at X-ray energies. We present analysis of the broad emission line fluxes and profiles, as well as the properties of the X-ray continuum and Fe Kα emission and we use these parameters to assess the consistency of our current AGN understanding.     

Inhibitory effect of simulated microgravity on differentiating preosteoblasts

The bone loss induced by microgravity is partly due to the decrease of mature osteoblasts. In the present study, we employed the random positioning machine (RPM) to simulate microgravity and investigated the acute effects of simulated microgravity on the differentiation of 2T3 preosteoblasts. Following 7 days’ culture under normal (1 g) condition, cells were exposed to simulated microgravity for 24 h. The results showed that 24 h treatment of simulated microgravity significantly decreased alkaline phosphatase (ALP) activity without changing the cell morphology. In addition, the mRNA expressions of osteogenic genes, including runt-related gene 2 (Runx2), osterix, osteocalcin (OC), type I collagen (Col I) and bone morphogenetic protein (BMP), were dramatically downregulated. Moreover, western blot analysis of total extracellular signal-regulated kinase (Erk) and phosphorylated Erk (p-Erk) indicated that p-Erk level, which represents the Erk activation status, was increased. Taken together, our results suggested that acute exposure to simulated microgravity inhibited osteoblast differentiation through modulating the expression of osteogenic genes and the Erk activity. These findings provide new insight for bone loss due to microgravity and unloading.     

Dynamical evolution of high area-to-mass ratio debris released into GPS orbits

A large set of simulations, including all the relevant perturbations, was carried out to investigate the long-term dynamical evolution of fictitious high area-to-mass ratio (A/M) objects released, with a negligible velocity variation, in each of the six orbital planes used by Global Positioning System (GPS) satellites. As with similar objects discovered in near synchronous trajectories, long lifetime orbits, with mean motions of about 2 revolutions per day, were found possible for debris characterized by extremely high area-to-mass ratios. Often the lifetime exceeds 100 years up to A/M ∼ 45 m²/kg, decreasing rapidly to a few months above such a threshold. However, the details of the evolution, which are conditioned by the complex interplay of solar radiation pressure and geopotential plus luni-solar resonances, depend on the initial conditions. Different behaviors are thus possible. In any case, objects like those discovered in synchronous orbits, with A/M as high as 20–40 m²/kg, could also survive in this orbital regime, with semi-major axes close to the semi-synchronous values, with maximum eccentricities between 0.3 and 0.7, and with significant orbit pole precessions (faster and wider for increasing values of A/M), leading to inclinations between 30° and more than 90°.     

Early effects of low dose C ion or X-ray irradiation on human peripheral blood lymphocytes

The aim of this study was to estimate the acute effects of low dose ¹²C⁶⁺ ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05 Gy ¹²C⁶⁺ ions or X-ray radiation and cell responses were measured at 24 h after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supernatant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-γ and TNF-α in HPBL and their protein levels in supernatant were significantly increased at 24 h after exposure to 0.05 Gy ¹²C⁶⁺ ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05 Gy high linear energy transfer (LET) ¹²C⁶⁺ radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDI.     

Study of atmospheric air pollutants during the partial solar eclipse on 15 January 2010 over Udaipur: A semi-arid location in Western India

The paper describes behavior of surface ozone, its precursor gases, BC along with TOCC, TWVC, AOT1020 nm as well as UV and IR radiation intensities observed during the partial solar eclipse of 15th January, 2010 over Udaipur, where 52% solar disc is obscured due to the moon’s shadow. During the beginning to main eclipse phase, the deviation values of several air pollutants concentrations from eclipse to control day values vary in a small range from −9 to −2 ppb in case of surface ozone and −180 to −80 ppb for CO. The corresponding change in the values of BC observed from −3.3 to −.5 μg/m³. No significant change is found in NO₂, NO or in ratio of NO₂/NO values during the partial eclipse time. TOCC values decrease from 3 to 5 DU along with a reduction in UV radiation intensity from 20 to 35% from starting to the main eclipse phase. The AOT1020 nm values are found to increase from .2 to 1.0 along with a reduction in IR radiation intensity order of 50%. However, TWVC values decrease from .22 to .1 cm during the eclipse hours. The low level of dilution in surface ozone in eclipse period may be attributed with change in local atmospheric boundary layer dynamic conditions or limited air pollutants dispersion, in term of decreases in planetary boundary layer height, wind speed and hence ventilation coefficient in the same eclipse hours. Thus, present studies support the argument for the leading roles of photochemical reactions with its precursor gases under presence of solar radiation in surface ozone variability. Other possible controlling factors are advection of air pollutants from the polluted region as evident from backward wind trajectories and altering the local meteorological conditions.     

Bacterial cellulose may provide the microbial-life biosignature in the rock records

Bacterial cellulose (BC) is a matrix for a biofilm formation, which is critical for survival and persistence of microbes in harsh environments. BC could play a significant role in the formation of microbial mats in pristine ecosystems on Earth. The prime objective of this study was to measure to what extent spectral and other characteristics of BC were changed under the performance of BC interaction with the earthly rock – anorthosite – via microorganisms. The spectral analyses (Fourier Transform Infrared FT-IR, spectroscopy, and atomic absorption spectroscopy) showed unprecedented accumulation of chemical elements in the BC-based biofilm. The absorption capacity of IR by BC was shielded a little by mineral crust formed by microorganisms on the BC-based biofilm surface, especially clearly seen in the range of 1200–900 cm⁻¹ in FT-IR spectra. Confocal scanning laser microscopy analysis revealed that elements bioleached from anorthosite created surface coats on the BC nanofibril web. At the same time, the vibrational spectra bands showed the presence of the characteristic region of anomeric carbons (960–730 cm⁻¹), wherein a band at 897 cm⁻¹ confirmed the presence of β-1, 4-linkages, which may serve as the cellulose fingerprint region. Results show that BC may be a biosignature for search signs of living organisms in rock records.     

Induction of genomic instability after an acute whole-body exposure of mice to Fe ions

The purpose of this study was to evaluate dose–response relationships for the in vivo induction of micronuclei (MN) as a measure of both initial radiation damage and the induction of genomic instability. These measurements were made in mouse blood erythrocytes as a function of radiation dose, radiation quality, time after irradiation, and the genetic background of exposed individuals. Blood samples were collected from two strains of mouse (CBA/CaJ and C57BL/6J) at different times up to 3 months following a whole-body exposure to various doses of 1 GeV/amu ⁵⁶Fe ions (0, 0.1, 0.5 and 1.0 Gy, at the dose rate of a 1 Gy/min) or ¹³⁷Cs gamma rays (0, 0.5, 1.0 and 3.0 Gy, at the dose rate of 0.72 Gy/min). Blood-smear slides were stained with acridine orange (AO). The frequencies of MN were measured in mature normochromatic-erythrocytes (MN-NCEs) and in immature polychromatic-erythrocytes (MN-PCEs). Effects of both types of radiation on erythropoiesis were also evaluated. As a measure of cell progression delay, a dose-dependent decrease in numbers of PCEs was observed at day 2 post-exposure in both strains, regardless of radiation quality. Subsequently, the levels of PCEs increased in all exposed mice, reaching control levels (or higher) by day 7 post-exposure. Further, at day 2 after the exposure, there was no increase in the frequency of MN-PCEs in CBA/CaJ mice exposed to ⁵⁶Fe ions while the frequency of MN-PCEs elevated as a function of dose in the C57BL/6J mice. At day 4, there was no dose related increase in MN-NCEs in either strain of mouse exposed to ¹³⁷Cs gamma rays. Additionally, at the early sacrifice times (days 2 and 4), ⁵⁶Fe ions were slightly more effective (per unit dose) in inducing MN-NCEs than ¹³⁷Cs gamma rays in CBA/CaJ mice. However, there was no increase in the frequency of MN-NCEs at late times after an acute exposure to either type of radiation. In contrast, both types of radiation induced increased MN-PCEs frequencies in irradiated CBA/CaJ mice, but not C57BL/6J mice, at late times post-exposure. This finding indicates the potential induction of genomic instability in hematopoietic cells of CBA/CaJ mice by both types of radiation. The finding also demonstrates the influence of genetic background on radiation-induced genomic instability in vivo.     

Simulation of depth–dose distributions for various ions in polyethylene medium

Study of depth–dose distributions for intermediate energy ion beams in tissue-like media such as polyethylene (CH₂)_n provides a good platform for further improvements in the fields of hadrontherapy and space radiation shielding. The depth–dose distributions for ¹²C ions at various energies and for light and intermediate ion beams (³He, ¹⁶O, ²⁰Ne and ²⁸Si) as well as for heavy ions ⁵⁶Fe in polyethylene were estimated by using simulation toolkit: Geant4. Calculations were performed mainly by considering two different combinations of standard electromagnetic (EM), binary cascade (BIC), statistical multifragmentation (SMF) and Fermi breakup (FB) models. The energies of the ion beams were selected to achieve the Bragg peaks at predefined position (∼60 mm) and as per their availability. Variations of peak-to-entrance ratio (from 7.44 ± 0.05 to 8.87 ± 0.05), entrance dose (from 2.89 ± 0.01 to 203.71 ± 0.63 MeV/mm) and entrance stopping power (from 3.608 to 208.858 MeV/mm, calculated by SRIM) with atomic number (Z) were presented in a systematic manner. The better peak-to-entrance ratio and less entrance dose in the region Z = 2 to 8 (i.e. ³He to ¹⁶O) may provide the suitability of the ion beams for hadrontherapy.     

Energy conversion of the flare due to direct electric fields from the sheared reconnection

In this paper we present a new mechanism of the main energy conversion of the solar flare. Since a flare inducing prominence (flux tube) rises V_z ? 300 km s⁻¹, the plasmas below it cannot continuously eject with Alfvén speeds of V_A = 3000 km s⁻¹ but probably with V_z ≈ ±100 km s⁻¹. Plasma up and downflows with V_A will within a short duration be blocked between the chromosphere where reconnected flux tubes are piling up, and the slowly rising flux rope. Hence the Petschek slow shock mechanism is difficult to be realized as a major energy converting mechanism.     

Evolution of the orbital elements for objects with high area-to-mass ratios in geostationary transfer orbits

A new population of uncatalogued objects in geosynchronous Earth orbits (GEO), with a mean motion of about 1 rev/day and eccentricities up to 0.6, has been identified recently. The first observations of this new type of objects were acquired in the framework of the European Space Agency’s (ESA) search for space debris in GEO and the geostationary transfer orbit (GTO) using the ESA 1-m telescope on Tenerife. Earlier studies have postulated that the perturbations due to the solar radiation pressure can lead to such large eccentricities for GEO objects with a high area-to-mass ratio (A/M). The simulations showed that the eccentricities of GEO objects with large A/M exhibit periodic variations with periods of about one year and amplitudes depending on the value of A/M. The findings of these studies could be confirmed by observations from the ESA 1-m telescope on Tenerife.     

Crop productivities and radiation use efficiencies for bioregenerative life support

NASA’s Biomass Production Chamber (BPC) at Kennedy Space Center was decommissioned in 1998, but several crop tests were conducted that have not been reported in the open literature. These include several monoculture studies with wheat, soybean, potato, lettuce, and tomato. For all of these studies, either 10 or 20 m² of plants were grown in an atmospherically closed chamber (113 m³ vol.) using a hydroponic nutrient film technique along with elevated CO₂ (1000 or 1200 μmol mol⁻¹). Canopy light (PAR) levels ranged from 17 to 85 mol m⁻² d⁻¹ depending on the species and photoperiod. Total biomass (DM) productivities reached 39.6 g m⁻² d⁻¹ for wheat, 27.2 g m⁻² d⁻¹ for potato, 19.6 g m⁻² d⁻¹ for tomato, 15.7 g m⁻² d⁻¹ for soybean, and 7.7 g m⁻² d⁻¹ for lettuce. Edible biomass (DM) productivities reached 18.4 g m⁻² d⁻¹ for potato, 11.3 g m⁻² d⁻¹ for wheat, 9.8 g m⁻² d⁻¹ for tomato, 7.1 g m⁻² d⁻¹ for lettuce, and 6.0 g m⁻² d⁻¹ for soybean. The corresponding radiation (light) use efficiencies for total biomass were 0.64 g mol⁻¹ PAR for potato, 0.59 g DM mol⁻¹ for wheat, 0.51 g mol⁻¹ for tomato, 0.46 g mol⁻¹ for lettuce, and 0.43 g mol⁻¹ for soybean. Radiation use efficiencies for edible biomass were 0.44 g mol⁻¹ for potato, 0.42 g mol⁻¹ for lettuce, 0.25 g mol⁻¹ for tomato, 0.17 g DM mol⁻¹ for wheat, and 0.16 g mol⁻¹ for soybean. By initially growing seedlings at a dense spacing and then transplanting them to the final production area could have saved about 12 d in each production cycle, and hence improved edible biomass productivities and radiation use efficiencies by 66% for lettuce (to 11.8 g m⁻² d⁻¹ and 0.70 g mol⁻¹), 16% for tomato (to 11.4 g m⁻² d⁻¹and 0.29 g mol⁻¹), 13% for soybean (to 6.9 g m⁻² d⁻¹ and 0.19 g mol⁻¹), and 13% for potato (to 20.8 g m⁻² d⁻¹ and 0.50 g mol⁻¹). Since wheat was grown at higher densities, transplanting seedlings would not have improved yields. Tests with wheat resulted in a relatively low harvest index of 29%, which may have been caused by ethylene or other organic volatile compounds (VOCs) accumulating in the chamber. Assuming a higher harvest index of 40% could be achieved by scrubbing VOCs, productivity of wheat seed could have been improved nearly 40% to 15.8 g m⁻² d⁻¹ and edible biomass radiation use efficiency to 0.30 g mol⁻¹.     

A simple model to predict solar radiation under clear sky conditions

Solar radiation is one of the major factors that dominate the thermal behaviors of aerostats in the daytime and the primary energy source of high altitude long endurance aerostats. Therefore, it is necessary to propose an accurate model to predict the solar irradiances. A comprehensive review of the well-known solar radiation models is conducted to help develop the new model. Based on the analysis of the existing models and the available radiation data, the extensive computer tests of the regression and optimization are conducted, from which the new solar radiation model for direct and diffuse irradiances under clear sky conditions is proposed. The new model has excellent prediction accuracy. The coefficient of determination for direct radiation is 0.992, with the root mean square error (RMSE) of 16.9 W/m² and the mean absolute error (MAE) of 2.2%. The coefficient of determination for diffuse radiation is 0.86, with RMSE = 8.7 W/m² and MAE = 9.9%. Comparisons with the well-known existing models show that the new model is much more accurate than the best existing ones.     

Experimental investigation of transient thermal behavior of an airship under different solar radiation and airflow conditions

Knowledge of the thermal behavior of airships is crucial to the development of airship technology. An experiment apparatus is constructed to investigate the thermal response characteristics of airships, and the transient temperature distributions of both hull and inner gas are obtained under the irradiation of a solar simulator and various airflow conditions. In the course of the research, the transient temperature change of the experimental airship is measured for four airflow speeds of 0 m/s (natural convection), 3.26 m/s, 5.5 m/s and 7.0 m/s, and two incident solar radiation values of 842.4 W/m² and 972.0 W/m². The results show that solar irradiation has significant influence on the airship hull and inner gas temperatures even if the airship stays in a ground airflow environment where the heat transfer is dominated by radiation and convection. The airflow around the airship is conducive to reduce the hull temperature and temperature nonuniformity. Transient thermal response of airships rapidly varies with time under solar radiation conditions and the hull temperature remains approximately constant in ∼5–10 min. Finally, a transient thermal model of airship is developed and the model is validated through comparison with the experimental data.     

Spectral analysis of time-integrated Konus–Wind GRBs: Implication on radiative mechanisms

We have analysed a sample of 328 time-integrated GRB prompt emission spectra taken via the Konus instrument on board the US GGS-Wind spacecraft between 2002 and 2004 using a couple of two-components models, Cut-off Power Law (CPL) + Power Law (PL) and blackbody (BB) + PL. The spectra show clear deviation from the Band function. The PL term is interpreted as the low energy tail of a nonthermal emission mechanism. The distributions of corresponding index β give values β < −2/3 consistent with synchrotron and synchrotron self-Compton mechanisms. The distribution of low energy index α associated with the CPL term shows clear discordance with synchrotron models for 31.4% of the analysed GRBs with values exceeding that for the line of death, α = −2/3. Then, a set of nonthermal radiation mechanisms producing harder slopes, i.e., α > −2/3, are presented and discussed. For the remaining majority (68.6%) of GRBs with CPL index α < −2/3, we show that optically thin synchrotron produced by a power law electron distribution of type, N(γ) ∼ γ^−p, γ₁ < γ < γ₂, for finite energy range (γ₂ ≠ ∞) is a likely emission mechanism with α ∼−(p + 1)/2 in the frequency range ν₁ ? ν ? ν₂ (where ν₂ = η²ν₁ with η = γ₂/γ₁), such that for p > 1/3, one gets α < −2/3. We also show that corresponding spectra in terms of F_ν and νF_ν functions are peaked around frequency ν₂ instead of ν₁, respectively for p < 1 and p < 3. Besides, thermal emission is examined taking a single Planck function for fitting the low energy range. It can be interpreted as an early emission from the GRB fireball photosphere with observed mean temperature, kT′ ∼ 16.8 keV. Furthermore, we have performed a statistical comparison between the CPL + PL and BB + PL models finding comparable χ²-values for an important fraction of GRBs, which makes it difficult to distinguish which model and specific radiation mechanism (possible thermal or nonthermal γ-ray emissions) are best suitable for describing the reported data. Therefore, additional information for those bursts, such as γ-ray polarization, would be highly desirable in future determinations of GRBs observational data.     

Neuroimmune response and sleep studies after whole body irradiation with high-LET particles

In order to investigate the biological effects of galactic rays on astronaut cerebral functions after space flight, mice were exposed to different heavy ions (HZE) in whole-body conditions at doses comparable to the galactic flux: ¹²C, ¹⁶O and ²⁰Ne (95 MeV/u, at 42–76 mGy). Animals were also exposed to 42 mGy of ⁶⁰Co radiation for comparison with HZE. The neuroimmune response, evaluated by interleukin-1 (IL-1) measurement, showed that this cytokine was produced 3 h after irradiation by ¹⁶O or ⁶⁰Co. In contrast, neither ¹²C (56.7 mGy) nor ²⁰Ne (76 mGy) induced IL-1 production. However, immunohistochemical staining of ¹²C-irradiated mouse brain tissue showed 2 months later a marked inflammatory reaction in the hippocampus and a diffuse response in parenchyma. Sleep studies were realized before and after exposure to 42 mGy of ¹⁶O and 76 mGy of ²⁰Ne: only the ²⁰Ne radiation displayed a small effect. A slight decrease in paradoxical sleep, corresponding to a reduction in the number of episodes of paradoxical sleep, was manifested between 8 and 22 days after exposure. Exposure to ¹²C and ¹⁶O induced no changes either in cellularity of spleen or thymus, or in caspase 3 activity (as much as four months after irradiation). Taken together, these data indicate that the CNS could be sensitive to heavy ions and that responses to HZE impact depend on the nature of the particle, the dose threshold and the time delay to develop biological processes. Differences in responses to different HZE highlight the complex biological phenomena to which astronauts are submitted during space flight.     

Solar cycle effect on temporal and spatial variation of topside ion density measured by SROSS C2 and ROCSAT 1 over the Indian longitude sector

We investigated the diurnal, seasonal and latitudinal variations of ion density Ni over the Indian low and equatorial topside ionosphere within 17.5°S to 17.5°N magnetic latitudes by combining the data from SROSS C2 and ROCSAT 1 for the 9 year period from 1995 to 2003 during solar cycle 23. The diurnal maximum density is found in the local noon or in the afternoon hours and the minimum occurs in the pre sunrise hours. The density is higher during the equinoxes as compared to that in the June and December solstice. The local time spread of the daytime maximum ion density increases with increase in solar activity. A north south asymmetry with higher ion density over northern hemisphere in the June solstice and over southern hemisphere in December solstice has been observed in moderate and high solar activity years. The crest to crest distance increases with increase in solar flux. Ion density bears a nonlinear relationship with F_10.7 cm solar flux and EUV flux in general. The density increases linearly with solar flux up to ∼150 sfu (1 sfu = 10⁻²²Wm⁻²Hz⁻¹) and EUV flux up to ∼50 units (10⁹ photons cm⁻² s⁻¹). But beyond this the density saturates. Inverse saturation and linear relationship have been observed in some season or latitude also. Inter-comparison of the three solar activity indices F_10.7 cm flux, EUV flux and F_10.7P (= (F_10.7 + F_10.7A)/2, where F_10.7A is the 81 day running average value of F_10.7) shows that the ion density correlates better with F_10.7P and F_10.7 cm fluxes. The annual average daytime total ion density from 1995 to 2003 follows a hysteresis loop as the solar cycle reverses. The ion density at 500 km over the Indian longitude sector as obtained by the international reference ionosphere is in general lower than the measured densities during moderate and high solar activity years. In low solar activity years the model densities are equal or higher than measured densities. The IRI EIA peaks are symmetric (±10°) in equinox while densities are higher at 10°N in June solstice and at 10°S in the December solstice. The model density follows F_10.7 linearly up to about F_10.7 > ∼150 sfu and then saturates.     

Tolerance of chufa (Cyperus esculentus L.) plants,representing the higher plant compartment in bioregenerative life support systems,to super-optimal air temperatures

Plants intended to be included in the photosynthesizing compartment of the bioregenerative life support system (BLSS) need to be studied in terms of both their production parameters under optimal conditions and their tolerance to stress factors that might be caused by emergency situations. The purpose of this study was to investigate tolerance of chufa (Cyperus esculentus L.) plants to the super-optimal air temperature of 45 ± 1 °C as dependent upon PAR (photosynthetically active radiation) intensity and the duration of the exposure to the stress factor. Chufa plants were grown hydroponically, on expanded clay, under artificial light. The nutrient solution was Knop’s mineral medium. Until the plants were 30 days old, they had been grown at 690 μmol m⁻² s⁻¹ PAR and air temperature 25 °C. Thirty-day-old plants were exposed to the temperature 45 °C for 6 h, 20 h, and 44 h at PAR intensities 690 μmol m⁻² s⁻¹ and 1150 μmol m⁻² s⁻¹. The exposure to the damaging air temperature for 44 h at 690 μmol m⁻² s⁻¹ PAR caused irreversible damage to PSA, resulting in leaf mortality. In chufa plants exposed to heat shock treatment at 690 μmol m⁻² s⁻¹ PAR for 6 h and 20 h, respiration exceeded photosynthesis, and CO₂ release in the light was recorded. Functional activity of photosynthetic apparatus, estimated from parameters of pulse-modulated chlorophyll fluorescence in Photosystem 2 (PS 2), decreased 40% to 50%. After the exposure to the stress factor was finished, functional activity of PSA recovered its initial values, and apparent photosynthesis (P_apparent) rate after a 20-h exposure to the stress factor was 2.6 times lower than before the elevation of the temperature. During the first hours of plant exposure to the temperature 45 °C at 1150 μmol m⁻² s⁻¹ PAR, respiration rate was higher than photosynthesis rate, but after 3–4 h of the exposure, photosynthetic processes exceeded oxidative ones and CO₂ absorption in the light was recorded. At the end of the 6-h exposure, P_apparent rate was close to that recorded prior to the exposure, and no significant changes were observed in the functional activity of PSA. At the end of the 20-h exposure, P_apparent rate was close to its initial value, but certain parameters of the functional activity of PSA decreased 25% vs. their initial values. During the repair period, the parameters of external gas exchange recovered their initial values, and parameters of pulse-modulated chlorophyll fluorescence were 20–30% higher than their initial values. Thus, exposure of chufa plants to the damaging temperature of the air for 20 h did not cause any irreversible damage to the photosynthetic apparatus of plants at either 690 μmol m⁻² s⁻¹ or 1150 μmol m⁻² s⁻¹ PAR, and higher PAR intensity during the heat shock treatment enhanced heat tolerance of the plants.     

The feasibility of using an L1 positioned dust cloud as a method of space-based geoengineering

In this paper a method of geoengineering is proposed involving clouds of dust placed in the vicinity of the L₁ point as an alternative to the use of thin film reflectors. The aim of this scheme is to reduce the manufacturing requirement for space-based geoengineering. It has been concluded that the mass requirement for a cloud placed at the classical L₁ point, to create an average solar insolation reduction of 1.7%, is 7.60 × 10¹⁰ kg yr⁻¹ whilst a cloud placed at a displaced equilibrium point created by the inclusion of the effect of solar radiation pressure is 1.87 × 10¹⁰ kg yr⁻¹. These mass ejection rates are considerably less than the mass required in other unprocessed dust cloud methods proposed and are comparable to thin film reflector geoengineering requirements. Importantly, unprocessed dust sourced in-situ is seen as an attractive scheme compared to highly engineered thin film reflectors. It is envisaged that the required mass of dust can be extracted from captured near Earth asteroids, whilst stabilised in the required position using the impulse provided by solar collectors or mass drivers used to eject material from the asteroid surface.