Influence of gravity on the circadian timing system.

The circadian timing system (CTS) is responsible for daily temporal coordination of physiological and behavioral functions both internally and with the external environment. Experiments in altered gravitational environments have revealed changes in circadian rhythms of species ranging from fungi to primates. The altered gravitational environments examined included both the microgravity environment of spaceflight and hyperdynamic environments produced by centrifugation. Acute exposure to altered gravitational environments changed homeostatic parameters such as body temperature. These changes were time of day dependent. Exposure to gravitational alterations of relatively short duration produced changes in both the homeostatic level and the amplitude of circadian rhythms. Chronic exposure to a non-earth level of gravity resulted in changes in the period of the expressed rhythms as well as in the phase relationships between the rhythms and between the rhythms and the external environment. In addition, alterations in gravity appeared to act as a time cue for the CTS. Altered gravity also affected the sensitivity of the pacemaker to other aspects of the environment (i.e., light) and to shifts of time cues. Taken together, these studies lead to the conclusion that the CTS is indeed sensitive to gravity and its alterations. This finding has implications for both basic biology and space medicine.     

Microgravity effects on sea urchin fertilization and development.

Gravity has been a pervasive influence on all living systems and there is convincing evidence to suggest that it alters fertilization and embryogenesis in several developmental systems. Notwithstanding the global importance of gravity on development, it has only been recently possible to begin to design experiments which might directly investigate the specific effects of this vector. The goal of this research program is to explore and understand the effects of gravity on fertilization and early development using sea urchins as a model system. Sea urchin development has several advantages for this project including the feasibility of maintaining and manipulating these cells during spaceflight, the high percentage of normal fertilization and early development, and the abundant knowledge about molecular, biochemical, and cellular events during embryogenesis which permits detailed insights into the mechanism by which gravity might interfere with development. Furthermore, skeletal calcium is deposited into the embryonic spicules within a day of fertilization permitting studies of the effects of gravity on bone calcium deposition.     

The biological clock of Neurospora in a microgravity environment.

The circadian rhythm of conidiation in Neurospora crassa is thought to be an endogenously derived circadian oscillation; however, several investigators have suggested that circadian rhythms may, instead, be driven by some geophysical time cue(s). An experiment was conducted on space shuttle flight STS-9 in order to test this hypothesis; during the first 7-8 cycles in space, there were several minor alterations observed in the conidiation rhythm, including an increase in the period of the oscillation, an increase in the variability of the growth rate and a diminished rhythm amplitude, which eventually damped out in 25% of the flight tubes. On day seven of flight, the tubes were exposed to light while their growth fronts were marked. Some aspect of the marking process reinstated a robust rhythm in all the tubes which continued throughout the remainder of the flight. These results from the last 86 hours of flight demonstrated that the rhythm can persist in space. Since the aberrant rhythmicity occurred prior to the marking procedure, but not after, it was hypothesized that the damping on STS-9 may have resulted from the hypergravity pulse of launch. To test this hypothesis, we conducted investigations into the effects of altered gravitational forces on conidiation. Exposure to hypergravity (via centrifugation), simulated microgravity (via the use of a clinostat) and altered orientations (via alterations in the vector of a 1 g force) were used to examine the effects of gravity upon the circadian rhythm of conidiation.     

Is the Earth’s orbital motion linked to the spin rotation of the Sun?

Time variations of the magnetic field of the Sun, seen as a star (the data 1968–2018, with more than 27 thousand daily measurements of the solar mean magnetic field), allowed to specify the rotation period of the gravitating solar mass: 27.027(6)?days, synodic. This indicates a presumably unknown physical connection between motions of the Sun and the Earth: in the course of a year our star accomplishes nearly 27 half-revolutions, while the planet itself performs an identical number of its spinnings during one complete axial revolution of the Sun. True origin of this strange Sun–Earth resonance is unknown, but it is supposed the phenomenon might be caused by slight coherent perturbations of gravity within the solar system.     

Parathyroid hormone-related protein is a gravisensor in lung and bone cell biology.

Parathyroid Hormone-related Protein (PTHrP) has been shown to be essential for the development and homeostatic regulation of lung and bone. Since both lung and bone structure and function are affected by microgravity, we hypothesized that 0 x g down-regulates PTHrP signaling. To test this hypothesis, we suspended lung and bone cells in the simulated microgravity environment of a Rotating Wall Vessel Bioreactor, which simulates microgravity, for up to 72 hours. During the first 8 hours of exposure to simulated 0 x g, PTHrP expression fell precipitously, decreasing by 80-90%; during the subsequent 64 hours, PTHrP expression remained at this newly established level of expression. PTHrP production decreased from 12 pg/ml/hour to 1 pg/ml/hour in culture medium from microgravity-exposed cells. The cells were then recultured at unit gravity for 24 hours, and PTHrP expression and production returned to normal levels. Based on these findings, we have obtained bones from rats flown in space for 2 weeks (Mission STS-58, SL-2). Analysis of PTHrP expression by femurs and tibias from these animals (n=5) revealed that PTHrP expression was 60% lower than in bones from control ground-based rats. Interestingly, there were no differences in PTHrP expression by parietal bone from space-exposed versus ground-based animals, indicating that the effect of weightlessness on PTHrP expression is due to the unweighting of weight-bearing bones. This finding is consistent with other studies of microgravity-induced osteoporosis. The loss of the PTHrP signaling mechanism may be corrected using chemical agents that up-regulate this pathway. In conclusion, PTHrP represents a stretch-sensitive paracrine signaling mechanism that may sense gravity.     

Farming in space: environmental and biophysical concerns.

The colonization of space will depend on our ability to routinely provide for the metabolic needs (oxygen, water, and food) of a crew with minimal re-supply from Earth. On Earth, these functions are facilitated by the cultivation of plant crops, thus it is important to develop plant-based food production systems to sustain the presence of mankind in space. Farming practices on earth have evolved for thousands of years to meet both the demands of an ever-increasing population and the availability of scarce resources, and now these practices must adapt to accommodate the effects of global warming. Similar challenges are expected when earth-based agricultural practices are adapted for space-based agriculture. A key variable in space is gravity; planets (e.g. Mars, 1/3 g) and moons (e.g. Earth's moon, 1/6 g) differ from spacecraft orbiting the Earth (e.g. Space stations) or orbital transfer vehicles that are subject to microgravity. The movement of heat, water vapor, CO2 and O2 between plant surfaces and their environment is also affected by gravity. In microgravity, these processes may also be affected by reduced mass transport and thicker boundary layers around plant organs caused by the absence of buoyancy dependent convective transport. Future space farmers will have to adapt their practices to accommodate microgravity, high and low extremes in ambient temperatures, reduced atmospheric pressures, atmospheres containing high volatile organic carbon contents, and elevated to super-elevated CO2 concentrations. Farming in space must also be carried out within power-, volume-, and mass-limited life support systems and must share resources with manned crews. Improved lighting and sensor technologies will have to be developed and tested for use in space. These developments should also help make crop production in terrestrial controlled environments (plant growth chambers and greenhouses) more efficient and, therefore, make these alternative agricultural systems more economically feasible food production systems.     

Influence of long-term altered gravity on the swimming performance of developing cichlid fish: including results from the 2nd German Spacelab Mission D-2.

This study presents qualitative and quantitative data concerning gravity-dependent changes in the swimming behaviour of developing cichlid fish larvae (Oreochromis mossambicus) after a 9 resp. 10 days exposure to increased acceleration (centrifuge experiments), to reduced gravity (fast-rotating clinostat), changed accelerations (parabolic aircraft flights) and to near weightlessness (2nd German Spacelab Mission D-2). Changes of gravity initially cause disturbances of the swimming performance of the fish larvae. With prolonged stay in orbit a step by step normalisation of the swimming behaviour took place in the fish. After return to 1g earth conditions no somersaulting or looping could be detected concerning the fish, but still slow and disorientated movements as compared to controls occurred. The fish larvae adapted to earth gravity within 3-5 days. Fish seem to be in a distinct early developmental stages extreme sensitive and adaptable to altered gravity; However, elder fish either do not react or show compensatory behaviour e.g. escape reactions.     

Effect of the seed perturbation amplitude on the equatorial spread F initiation during solar minimum

The contribution of gravity wave (GW) to the initiation/development of spread F during a solar minimum year was investigated through the comparison of the observed precursory parameters and characteristics of the corresponding equatorial spread F (ESF) events. The ionospheric parameters were recorded at the magnetic equatorial station Sao Luis (2.3°S, 44°W, dip latitude 2°S) during March and October 2010. These data were used to estimate the influence of the relative gravity wave amplitude and the ambient ionospheric condition on the diurnal variation of the spread F initiation. The vertical velocity drift indicated a clear control and defines the threshold for the seasonal variability of the ESF occurrence. However, it was insufficient to solely determine or predict the day to day variation of ESF occurrence. Thus, few days with contrasting ambient ionospheric condition and magnitude of GW amplitude were analysed in order to investigate the role of the different precursory factors in the observed diurnal variation of the plasma irregularity development. The density scale length and gravity wave amplitude were shown to immensely contribute to the linear instability growth rate, especially during the days with a low post-sunset rise. Thus, the experimental observations have demonstrated the strong inter-dependence between the precursory factors and they have also highlighted the probable control of the ESF morphology.     

Dust grain orbital behavior around ceres

Many asteroids show indications they have undergone impacts with meteoroid particles having radii between 0.01 m and 1 m. During such impacts, small dust grains will be ejected at the impact site. The possibility of these dust grains (with radii greater than 2.2 μm) forming a halo around a spherical asteroid (such as Ceres) is investigated using standard numerical integration techniques. The orbital elements, positions, and velocities are determined for particles with varying radii taking into account both the influence of gravity, radiation pressure, and the interplanetary magnetic field (for charged particles). Under the influence of these forces it is found that dust grains (under the appropriate conditions) can be injected into orbits with lifetimes in excess of one year. The lifetime of the orbits is shown to be highly dependent on the location of the ejection point as well as the angle between the surface normal and the ejection path. It is also shown that only particles ejected within 10° relative to the surface tangential survive more than a few hours and that the longest-lived particles originate along a line perpendicular to the Ceres-Sun line.     

Biorhythms on cellular and organismic level (introduction).

The regular change of day and night, of light and darkness during millions of years has strongly affected the development of life on earth. Many organisms adapted themselves to this environmental condition and, finally, evolved an endogenous timer which usually is in phase with the earth's rotation and causes many functions to perform one oscillation per day. Such circadian rhythms (derived from circa dies i.e. about 1 day) were found in almost all classes of plants and animals, and even in protozoans. They persist in a constant environment and, therefore, are independent of any known external trigger signals. Since even unicells perform circadian rhythms which are similar to those observed in highly developed multicellular organisms many scientists favor the existence of a basic mechanism common to all kinds of biological clocks that is located somewhere in the single cell and probably comprises many different biochemical reactions. One purpose of this topical meeting was to discuss how organisms respond to the absence of gravity and terrestrial zeitgeber and how they may react to the imposing of hypergravity fields. Another aim was to develop model-mechanisms appropriate to describe these responses.     

The simulation of lunar gravity field recovery from D-VLBI of Chang’E-1 and SELENE lunar orbiters

The lunar gravity field is a foundation to study the lunar interior structure, and to recover the evolution history of the Moon. It is still an open and key topic for lunar science. For above mentioned reasons, it becomes one of the important scientific objectives of recent lunar missions, such as KAGUYA (SELENE) the Japanese lunar mission and Chang’E-1, the Chinese lunar mission. The Chang’E-1 and the SELENE were successfully launched in 2007. It is estimated that these two missions can fly around the Moon longer than 6 months simultaneously. In these two missions, the Chinese new VLBI (Very Long Baseline Interferometry) network will be applied for precise orbit determination (POD) by using a differential VLBI (D-VLBI) method during the mission period. The same-beam D-VLBI technique will contribute to recover the lunar gravity field together with other conventional observables, i.e. R&RR (Range and Range Rate) and multi-way Doppler. Taking VLBI tracking conditions into consideration and using the GEODYNII/SOVLE software of GSFC/NASA/USA [8 and 10], we simulated the lunar gravity field recovering ability with and without D-VLBI between the Chang’E-1 and SELENE main satellite. The cases of overlapped flying and tracking period of 30 days, 60 days and 90 days have been analyzed, respectively. The results show that D-VLBI tracking between two lunar satellites can improve the gravity field recovery remarkably. The results and methods introduced in this paper will benefit the actual missions.     

Estimating low resolution gravity fields at short time intervals to reduce temporal aliasing errors

The Gravity Recovery and Climate Experiment (GRACE) satellite mission has been estimating temporal changes in the Earth’s gravitational field since its launch in 2002. While it is not yet fully resolved what the limiting source of error is for GRACE, studies on future missions have shown that temporal aliasing errors due to undersampling signals of interest (such as hydrological variations) and errors in atmospheric, ocean, and tide models will be a limiting source of error for missions taking advantage of improved technologies (flying drag-free with a laser interferometer). This paper explores the option of reducing the effects of temporal aliasing errors by directly estimating low degree and order gravity fields at short time intervals, ultimately resulting in data products with improved spatial resolution. Three potential architectures are considered: a single pair of polar orbiting satellites, two pairs of polar orbiting satellites, and a polar orbiting pair of satellites coupled with a lower inclined pair of satellites. Results show that improvements in spatial resolution are obtained when one estimates a low resolution gravity field every two days for the case of a single pair of satellites, and every day for the case of two polar pairs of satellites. However, the spatial resolution for these cases is still lower than that provided by simply destriping and smoothing the solutions via standard GRACE post-processing techniques. Alternately, estimating daily gravity fields for the case of a polar pair of satellites coupled with a lower inclined pair results in solutions with superior spatial resolution than that offered by simply destriping and smoothing the solutions.     

Influence of gravitoinertial force on vestibular nystagmus in man observed in a centrifuge.

The influence of gravity load on the vestibular system in man was investigated in a centrifuge operating on the free swing principle. The vertical vestibular nystagmus induced by acceleration to 3G was analyzed and compared with reference measurements during 1G. Our data indicate that the effects of increased gravity load include a prolonged decay time constant of upbeat nystagmus and a subject-dependent persisting upbeat nystagmus. In an attempt to explain these findings, an extension of the velocity storage model is proposed, with gravity as a second stimulus function in addition to angular acceleration.     

Gravity waves and wind-shear in the MLT at 23°S

We have used the technique suggested by Hocking [Hocking, W. A new approach to momentum flux determinations using SKiYMET meteor radars. Ann. Geophys. 23, 2005.] to derive short period wind variances in the 80–100 km region from meteor radar data. We find that these fluctuating winds, assumed to correspond to gravity waves and turbulence, are closely correlated with the vertical shear of the horizontal tidal winds. This close correlation suggests that in situ wind shear may be a major source of gravity waves and turbulence in the MLT. If this is the case, gravity waves generated in the troposphere and propagating up to the MLT region, generally assumed to constitute an important influence on the climatology of the region, may be a less important source of energy and momentum in the 80–100 km region than has been hitherto believed.     

变重力场中散体颗粒堆的安息角及接触力分布

为研究重力与散体颗粒堆安息角的关系,针对颗粒系统的随机性和离散性特点,通过三维离散单元法建立颗粒的运动模型,对颗粒在不同重力场中的堆积过程进行了模拟,得出了颗粒堆的安息角及接触力概率分布.结果表明:颗粒堆内接触力构成的力链呈非规则网络状,其中,接触力近似对数正态分布,约65%接触力低于平均值;接触点中约70%处于临界滑移,其余接触点切向力与法向力的比值为均匀分布;不同重力场中颗粒堆接触力的分布规律具有相似性,即接触力相对重力无量纲化之后,其分布函数高度相符;颗粒堆的微观结构具有随机性差异,但是安息角不受重力大小的影响.     

强震前异常电场激发声重波对电离层的影响

震前地震孕育期地表异常增强的电场,通过大气电导率传输到电离层高度.该异常电场通过非稳态局部加热,可以在电离层高度激发声重波.基于该理论,利用一维时变中纬电离层物理模型,模拟了该扰动源对电离层电子密度的影响.结果表明,重力波引起的中性风速度扰动对电离层电子密度分布影响甚微,该机理无法解释震前电离层异常扰动现象.      

Possible actions of gravity on the cellular machinery.

Since the first flight of the ESA Biorack on the German Spacelab Mission D1 in 1985 evidence has been obtained that biological cells and small unicellular organisms function differently under conditions of microgravity. However, there is still lack of scientific proof that these effects are caused by a direct influence on the cells in the weightlessness condition. The question how normal gravity may play a role in cellular activity is being addressed and the results show that gravity may provide important signals during certain state transitions in the cell. These would be gravity-sensitive windows in the biological process. Also, by amplification mechanisms inside the cell, the cell may assume a state that is typical for normal gravity conditions and would change in microgravity. Experimental tools are discussed that would provide the conditions to obtain evidence for direct action of gravity and for the possible existence of gravity-sensitive windows.     

背景风场对中尺度受导重力波的影响

采用重力波的大气分层全波解法，计算得出了强逆风场作用下以及无风条件下中尺度重力波受导波模的色散曲线和衰减距离曲线，结果表明，强逆风场可以使得各中尺度受导波模衰减距离明显变长，并导致不同尺度重力波的衰减距离出现峰值分布，通过分析美国MillstoneHill台站和武汉电离层观象中的TID实测结果证实了这些风场支持的远距离传播波模的存在。     

Light microscopic analysis of the gravireceptor in Xenopus larvae developed in hypogravity.

The paper describes an investigation of the influence of gravity on the early differentiation of gravity receptors in Xenopus embryos and larvae. There is evidence that the expression of crystals in the saccus endolymphaticus was statistically greater when the embryos developed in near weightlessness (hypogravity) than on earth. The function of these crystals is unknown but they may contribute to the functioning of the vestibular apparatus.     

Histochemical investigations on the influence of long-term altered gravity on the CNS of developing cichlid fish: results from the 2nd German Spacelab Mission D-2.

The effect of long-term (10 days) altered gravitational conditions upon succinate dehydrogenase (SDH) reactivity in total brains as well as in individual brain nuclei of developing cichlid fish larvae had been investigated by means of semiquantitative histochemical methods (densitometric grey value analysis). Increasing accelerations from near weightlessness (spaceflight) via 1g controls to 3g hyper gravity (centrifuge) resulted in slightly increasing "all over the brain" (total brain) SDH reactivity. When focusing on distinct neuronal integration centers within the same brains in order to find the anatomical substratum of the gross histochemical data, significant effects of altered gravity only within vestibulum related brain parts were obtained.