Longevity in space; experiment on the life span of Paramecium cell clone in space.

Life span is the most interesting and also the most important biologically relevant time to be investigated on the space station. As a model experiment, we proposed an investigation to assess the life span of clone generation of the ciliate Paramecium. In space, clone generation will be artificially started by conjugation or autogamy, and the life span of the cell populations in different gravitational fields (microgravity and onboard 1 x g control) will be precisely assessed in terms of fission age as compared with the clock time. In order to perform the space experiment including long-lasting culture and continuous measurement of cell division, we tested the methods of cell culture and of cell-density measurement, which will be available in closed environments under microgravity. The basic design of experimental hardware and a preliminary result of the cultivation procedure are described.     

Evaluation of gravity-dependent membrane potential shift in Paramecium

It is still debated whether or not gravity can stimulate unicellular organisms. This question may be settled by revealing changes in the membrane potential in a manner depending on the gravitational forces imposed on the cell. We estimated the gravity-dependent membrane potential shift to be about 1 mV G⁻¹ for Paramecium showing gravikinesis at 1–5 G, on the basis of measurements of gravity-induced changes in active propulsion and those of propulsive velocity in solutions, in which the membrane potential has been measured electrophysiologically. The shift in membrane potential to this extent may occur from mechanoreceptive changes in K⁺ or Ca²⁺ conductance by about 1% and might be at the limit of electrophysiological measurement using membrane potential-sensitive dyes. Our measurements of propulsive velocity vs membrane potential also suggested that the reported propulsive force of Paramecium measured in a solution of graded densities with the aid of a video centrifuge microscope at 350 G was 11 times as large as that for −29 mV, i.e., the resting membrane potential at [K⁺]_o = 1 mM and [Ca²⁺]_o = 1 mM, and, by extrapolation, that Paramecium was hyperpolarized to −60 mV by gravity stimulation of 100- G equivalent, the value corrected by considering the reduction of density difference between the interior and exterior of the cell in the graded density solution. The estimated shift of the membrane potential from −29 mV to −60 mV by 100- G equivalent stimulation, i.e., 0.3 mV G⁻¹, could reach the magnitude entirely feasible to be measured more directly.     

The minimal cost of life in space.

The cost of keeping people alive in space is assessed from a theoretical viewpoint and using two actual designs for plant growth systems. While life support is theoretically not very demanding, our ability to implement life support is well below theoretical limits. A theoretical limit has been calculated from requirements and the state of the art for plant growth has been calculated using data from the BIO-Plex PDR and from the Cornell CEA prototype system. The very low efficiency of our current approaches results in a high mission impact, though we can still see how to get a significant reduction in cost of food when compared to supplying it from Earth. Seeing the distribution of costs should allow us to improve our current designs.     

Optical and electron-microscopic studies of the Funaria hygrometrica protonema after cultivation for 96 days in space

$\text{Funaria hygrometrica}$ protonema cells grown in the “IFS-2” (Inoculating fixing system) for 96 days on board the Salyut 6 — Soyuz 32 orbital scientific station were examined by light and electron-microscopy. Investigation of experimental and control cells of the moss protonema showed common features as well as distinctions in their structure. Protonema cells of $\text{Funaria hygrometrica}$ both differentiate and undergo photosynthesis during space flight. Changes in cell shape, decreased cell size, a reduction in the volume of starch granules, and altered chloroplast structure were observed.     

Enzyme activities and membrane lipids in Artemia cysts after a long duration space flight

In the Free Flyer Biostack Experiment (L.D.E.F. mission) investigations have shown that biological objects in a resting state can survive more than 5.5 years of exposure to the space factors in particular microgravity and cosmic rays. We have measured enzyme activities involved in metabolic pathways of sugar and lipid degradation and determined phospholipid composition. Pyruvate kinase and glucose-6-phosphate dehydrogenase activities in space-exposed cysts were higher than in earth controls after 1 hour incubation. In controls, total phospholipids remained unchanged, on the contrary they increased significantly in space-exposed cysts. The rate of metabolism of various phospholipid components was unchanged in controls allowing the development while the level of most of them decreased in space-exposed cysts except for phosphatidylcholine. Enzyme activities (acetylhydrolase, phospholipase A₂ and lyso phospholipase) involved in phospholipid degradation increased; however, activities were much higher in space-exposed cysts. In conclusion, the long duration space flight resulted in an increase of the metabolic activity correlated with a faster development within the first 20 hours of post flight incubation.     

Survey of the vestibulum, and behavior of xenopus laevis larvae developed during a 7-days space flight

Aquatic animals have almost no body weight related proprioception for spatial orientation. larvae, like fish, maintain their attitude in water by continuous correction with their fin(s). For these reasons a special performance of the equilibrium system compared to terrestrial animals is necessary. Evidently fish therefore have more compact (dense) otoliths; larvae have less dense otolith (membranes) similar to land vertebrates; but their sacculus-otoliths are vertically positioned, which also may lead to a higher g-sensitivity.

For plausibility reasons gravity should influence the embryonic development of gravity receptors. Yet, evaluations of photographs taken from the surface of cut deep-frozen objects by incident light show no aberration of the shape of the whole vestibulum and of the shape, density, size and position of the otolith membrane in larvae developed under near-zero g (NEXPA-BW-STATEX in D1-Mission).

The further evaluation of the “weightless-larvae” revealed a probably not yet described statolith-like formation in the dorsal wall of the vestibulum. In the weightless larvae this formation outnumbers, also qualitatively, strongly the 1-g controls.

An extra result is the lack of striking effects of cosmic radiation on the embryonic development of the flown eggs.

The swimming behavior of the larvae which was observed about one hour after landing of the Space Shuttle showed a typical anomaly (loop swimming), which is known from larvae developed on the clinostat or from fish flown aboard Apollo capsules.     

Improvements in and actual performance of the Plant Experiment Unit onboard Kibo,the Japanese experiment module on the international space station

In 2004, Japan Aerospace Exploration Agency developed the engineered model of the Plant Experiment Unit and the Cell Biology Experiment Facility. The Plant Experiment Unit was designed to be installed in the Cell Biology Experiment Facility and to support the seed-to-seed life cycle experiment of Arabidopsis plants in space in the project named Space Seed. Ground-based experiments to test the Plant Experiment Unit showed that the unit needed further improvement of a system to control the water content of a seedbed using an infrared moisture analyzer and that it was difficult to keep the relative humidity inside the Plant Experiment Unit between 70 and 80% because the Cell Biology Experiment Facility had neither a ventilation system nor a dehumidifying system. Therefore, excess moisture inside the Cell Biology Experiment Facility was removed with desiccant bags containing calcium chloride. Eight flight models of the Plant Experiment Unit in which dry Arabidopsis seeds were fixed to the seedbed with gum arabic were launched to the International Space Station in the space shuttle STS-128 (17A) on August 28, 2009. Plant Experiment Unit were installed in the Cell Biology Experiment Facility with desiccant boxes, and then the Space Seed experiment was started in the Japanese Experiment Module, named Kibo, which was part of the International Space Station, on September 10, 2009 by watering the seedbed and terminated 2 months later on November 11, 2009. On April 19, 2010, the Arabidopsis plants harvested in Kibo were retrieved and brought back to Earth by the space shuttle mission STS-131 (19A). The present paper describes the Space Seed experiment with particular reference to the development of the Plant Experiment Unit and its actual performance in Kibo onboard the International Space Station. Downlinked images from Kibo showed that the seeds had started germinating 3 days after the initial watering. The plants continued growing, producing rosette leaves, inflorescence stems, flowers, and fruits in the Plant Experiment Unit. In addition, the senescence of rosette leaves was found to be delayed in microgravity.     

Measuring the matter distribution within z = 0.2 cluster lenses with XMM-Newton

We present an analysis of seven clusters observed by XMM-Newton as part of our survey of 17 most X-ray luminous clusters of galaxies at z  0.2 selected for a comprehensive and unbiased study of the mass distribution in massive clusters. Using the public software FTOOLS and XMMSAS we have set up an automated pipeline to reduce the EPIC MOS and pn spectro-imaging data, optimized for extended sources analysis. We also developped a code to perform intensive spectral and imaging analysis particularly focussing on proper background estimate and removal. XMM-Newton deep spectro-imaging of these clusters allowed us to fit a standard β-model to their gas emission profiles as well as a standard MEKAL emission model to their extracted spectra, and test their inferred characteristics against already calibrated relations.     

Space radiation quality factor investigations with “NAUSICAA”-device on board the “MIR” space station

The estimation of radiation quality factor in space flights is a serious problem of space dosimetry. The solution of this problem is based on LET-spectra measurements. The “NAUSICAA”-device installed on the “MIR” station has a detector with a large geometric factor, that gives the possibility of measuring LET-spectra with sufficient statistic in relatively short time interval and hence the corresponding Q. LET-spectra are accumulated through 24 hour periods and equivalent dose (H), absorbed dose (D), quality factor and flux (F) are measured every 10 minutes. The obtained data permit the estimate of the diurnal Q and Q in South Atlantic Anomaly. These values vary in the range of 1.4 – 2.2. The analysis of these values including their comparison with the calculated results is given in this paper.     

Thermal properties of molten InSb,GaSb, and InxGa1−xSb alloy semiconductor materials in preparation for crystal growth experiments on the international space station

The thermal properties of InSb, GaSb and In_xGa_1−xSb, such as the viscosity, wetting property, and evaporation rate, were investigated in preparation for the crystal growth experiment on the International Space Station (ISS). The viscosity of InGaSb, which is an essential property for numerical modeling of crystal growth, was evaluated. In addition, the wetting properties between molten In_xGa_1−xSb and quartz, BN, graphite, and C-103 materials were investigated. The evaporation rate of molten In_xGa_1−xSb was measured to determine the affinity of different sample configurations. From the measurements, it was found that the viscosity of In_xGa_1−xSb was between that of InSb and GaSb. The degree of wetting reaction between molten In_xGa_1−xSb and the C-103 substrate was very high, whereas that between molten In_xGa_1−xSb and quartz, BN, and graphite substrates was very low. The results suggest that BN and graphite can be used as materials to cover InSb and GaSb samples inside a quartz ampoule during the microgravity experiments. In addition, the difference of the evaporation rate of molten In_xGa_1−xSb, GaSb, and InSb was small at low, and large at high temperature.     

Effects of space flight exposure on cell growth,tumorigenicity and gene expression in cancer cells

It is well recognized that harsh outer space environment, consisting of microgravity and radiation, poses significant health risks for human cells. To investigate potential effects of the space environment exposure on cancer cells we examined the biological changes in Caski cells carried by the “Shen Zhou IV” spaceship. After exposure for 7 days in spaceflight, 1440 survival subclonal cell lines were established and 4 cell lines were screened. 44F10 and 17E3 were selected because of their increased cell proliferation and tumorigenesis, while 48A9 and 31F2 had slower cytological events. Experiments with cell proliferation assay, flow cytometry, soft agar assay, tumorigenesis assay and DNA microarray analysis have shown that selected cell lines presented multiple biological changes in cell morphology, cell growth, tumorigenicity and gene expression. These results suggest that space environment exposure can make significant biological impact on cancer cells and provide an entry point to find the immunological target of tumorigenesis.     

ROSAT observations of the Chamaeleon star forming cloud

Two soft X-ray images of the Chamaeleon I star forming cloud obtained with the ROSAT Position Sensitive Proportional Counter are presented. Seventy reliable, and perhaps 19 additional, X-ray sources are found. Up to Ninety percent of these sources are certainly or probably identified with T Tauri stars formed in the cloud. Twenty to 35 are probably previously unrecognized ‘weak’ T Tauri (WTT) stars. T Tauri X-ray luminosities range from log , or 10² – 10⁴ times solar levels, with mean in the 0.2–2.5 keV band. The X-ray luminosities of well-studied Chamaeleon cloud members are correlated with a complex of four stellar properties: effective temperature, mass, radius and bolometric luminosity. The spatial distribution, H-R diagram locations of the stars indicate WTT and CTT are coeval. The total premain sequence population of the cloud is likely to be > 100 stars, with WTT stars outnumbering ‘classical’ T Tauri (CTT) stars by 2:1.     

Evolution of coronal mass ejection/shock system in interplanetary space

The evolution of coronal mass ejection/shock system is investigated by numerically solving the usual set of two-dimensional single-fluid polytropic magnetohydrodynamic equations from 1 R_s to 1 AU in the meridian plane. The simulation result reveals that the coronal mass ejection/shock system formed near the sun evolves into the magnetic cloud/shock system near the earth’s orbit through the following three phases: the initial formation, the dominant latitudinal expansion and the similar expansion.     

The role of the space industry in building capacity in emerging space nations

The space industry in established space-faring nations is playing an increasingly important role in the development of capacity in emerging space nations. The role of industry ranges from provision of turn-key systems, to provision of training and joint development of satellites in partnership with emerging space countries. Ranked number 1 worldwide in terms of satellites ordered in 2006, Thales Alenia Space is at the heart of the most high performance satellite technologies in both civil and defense sectors. The company has been involved in parterships with a number of emerging space nations. This paper discusses several key factors for successful interaction of industry with national space programmes in emerging space nations.     

Present results of the joint radiobiological ESA/IBMP experiments “seeds” aboard Cosmos 2044 and 2229 - correlation of micro-dosimetric data and damage endpoints in Arabidopsis thaliana plants

Experimental data obtained from two Cosmos missions (2044, 2229) performed with dry plant seeds of Arabidopsis thaliana in a Biostack configuration are compared. Biological stress and genetic risk are induced in dry seeds by cosmic radiation, especially the high energetic, densely ionizing component of eavy ons (HI). Subdivision of impacting HI particles into LET-groups (15–35; 35–100; >100 keV/μm) showed the contribution of each LET group to the induction of different biological damage endpoints (survival, cell transformation, lethal plant development). An attempt is presented to comprehend the influence of the spatial energy deposition as a biophysical HI track parameter.     

First astrometric observations of space debris with the MéO telescope

The MéO (for Métrologie Optique) telescope is the Satellite and Lunar Laser Ranging (SLR) dedicated telescope of Observatoire de la Côte d’Azur (France) located at plateau de Calern. The telescope uses an altazimuth mount. The motorization of the mount has a capability of 6 deg/s allowing the follow up of Low Earth Orbits (LEO) satellites, as well as Medium Earth Orbits (MEO) and geostationary (GEO) satellites, and the Moon. The telescope has a primary mirror of 1.54 m. It uses a Nasmyth focus equipped with an EMCCD camera. The telescope field of view, defined by the equivalent focal length and the size of the camera, is currently 3.4 arcmin × 3.4 arcmin.     

Status of the T2L2/Jason2 Experiment

The T2L2 (Time Transfer by Laser Link) project, developed by CNES and OCA will permit the synchronization of remote ultra stable clocks and the determination of their performances over intercontinental distances. The principle of the experiment derives from Satellite Laser Ranging (SLR) technology with dedicated space equipment. T2L2 was accepted in 2005 to be on board the Jason2 altimetry satellite. The payload consists of both event timer and photo detection modules. The system uses the ultra-stable quartz oscillator of DORIS as on-board reference clock on one hand, and the Laser Reflector Array, making T2L2 a real two-way time transfer system on the other hand. The expected time stability of the T2L2 instrument (detection and timing), referenced by the DORIS oscillator and including all internal error sources should be at the level of 10–12 ps at 1 s and <1 ps at 1000 s. The metrological specifications of T2L2 should permit to maintain a precision of 1 to a few ps when measuring the phase of a clock during around 1000 seconds.     

Implications of ROSAT observations for the local hot bubble

The ROSAT all-sky survey and Guest Observer pointed observations have brought a wealth of high-quality data to the study of the soft X-ray diffuse background. Analysis of the spatial structure of the 1/4 keV flux, including observations of shadows cast by discrete clouds in the interstellar medium, have allowed the separation of the observed flux into foreground and background components supporting a spatially varying local component consistent with previous ideas of the local hot bubble, a highly variable galactic halo component, and an extragalactic background.