UV photobiochemistry under space conditions

The response of spores of Bacillus subtilis, cells of Deinococcus radiodurans and conidia of Aspergillus ochraceus to actual and simulated space conditions (UV in combination with long-term exposure to extremely dry conditions, including vacuum) has been studied: The following effects have been analyzed: decrease of viability, occurrence of DNA double strand breaks, formation of DNA-protein cross-links and DNA-DNA cross-links. All organisms show an increased sensitivity to UV light in extreme dryness (dry argon or vacuum) compared to an irradiation in aqueous suspension. The UV irradiation leads in all cases to a variety of DNA lesions. Very conspicuous is the occurrence of double strand breaks. Most of these double strand breaks are produced by incomplete repair of other lesions, especially base damages. The increase in DNA lesions can be correlated to the loss in viability. The specific response of the chromosomal DNA to UV irradiation in extreme dryness, however, varies from species to species and depends on the state of dehydration. The formation of DNA double strand breaks and DNA-protein cross-links prevails in the case of B. subtilis spores. In cells of Deinococcus radiodurans DNA-DNA cross-links often predominate, in conidia of Aspergillus ochraceus double strand breaks. The results obtained by direct exposure to space conditions (EURECA mission and D2 mission) largely agree with the laboratory data.     

Microbial processes in frozen food

Deep freezing of food and storage at −19°C is a standard conservation procedure in food technology. The lower limit of growth of bacteria in food is from about −5°C to about −8°C, whereas the reproduction limit of yeasts is 2 to 3°C lower. Storage temperatures above −10°C should therefore not be used. At −18°C, a commonly used storage temperature, no growth of microorganisms will occur. The microorganisms mainly found at the lower growth limit are Pseudomonas sp. and basidiomycete yeasts. The reduction in the number of microorganisms due to freezing, storage, and thawing is not of practical importance. Microbial enzymes, in particular lipases and proteases, are still active at −18°C. Therefore, the quality of raw products and good hygiene at the production site are most important.     

Molecular and biophysical aspects of adaptation of life to temperatures below the freezing point

This review examines adaptation of life to temperatures below −2°C, from a biophysical point of view and relative to the properties of water. Different metabolic processes have different lowest temperatures. The lowest established temperature for photosynthesis and growth seems to be about −17°C. The review discusses membrane lipids from Antarctic microbial systems, and the limits to longevity of life in the cold, with coverage of permafrost microbial systems. It is directed towards readers of many backgrounds and supplements an earlier survey (L. Finegold, Adv. Space Res. 6, 257–264, 1986).     

Vega project — Three channel spectrophotometer for the Halley''s comet investigation

A spectrometer for the Halley's Comet Investigation after the VEGA Project is described in the present work. It consists of a telescope and three spectral channels: UV (120 – 290 nm), resolution Δ λ / λ = 170; VIS (280 – 710 nm), resolution Δ λ / λ = 170; IR (950 – 1900 nm), resolution Δ λ / λ = 70.

With the help of two-coordinate scanner, the secondary mirror of the telescope allows spatial scanning of the Comet with a frame 2°×1,5° with 105 different pixels.     

High temperature alters the growth reaction of Pottia protonemata.

Under gravistimulation, dark-grown protonemata of Pottia intermedia revealed negative gravitropism with a growth rate of approximately 28 μm·h⁻¹ at room temperature (20 °C). In 7 days, the protonema formed a bundle of vertically oriented filaments. At an elevated temperature (30 °C), bundles of vertically growing filaments were also formed. However, both filament growth rate and amplitude of the gravicurvature were reduced. Red light (RL) irradiation induced a positive phototropism of most apical protonemal cells at 20 °C. In a following period of darkness, approximately two-thirds of such cells began to grow upward again, recovering their negative gravitropism. RL irradiation at the elevated temperature caused a partial increase in the number of protonemal cells with negative phototropism, but the protonemata did not exhibit negative gravitropism after transfer to darkness. The negative gravitropic reaction was renewed only when protonemata were placed at 20 °C. A dramatic decrease in starch amount in protonemal apical cells, which are sensitive to both gravity and light, occurred at the higher temperature. Such a decrease may be one of the reasons for the inhibition of the protonemal gravireaction at the higher temperature. The observation has a bearing on the starch-statolith theory.     

Cold resistance and metabolic activity of lichens below 0°C

Laboratory measurements show that lichens are extremely tolerant of freezing stress and of low-temperature exposure. Metabolic activity recovered quickly after severe and extended cold treatment. Experimental results demonstrate also that CO₂ exchange is already active at around −20°C. The psychrophilic character of polar lichen species is demonstrated by optimum temperatures for net photosynthesis between 0 and 15°C. In situ measurements show that lichens begin photosynthesizing below 0°C if the dry thalli receive fresh snow. The lowest temperature measured in active lichens was −17°C at a continental Antarctic site. The fine structure and the hydration state of photobiont and mycobiont cells were studied by low-temperature scanning electron microscopy (LTSEM) of frozen hydrated specimens. Water potentials of the frozen system are in the range of or even higher than those allowing dry lichens to start photosynthesis by water vapor uptake at +10°C. The great success of lichens in polar and high alpine regions gives evidence of their physiological adaptation to low temperatures. In general lichens are able to persist through glacial periods, but extended snow cover and glaciation are limiting factors.     

Effects of salts and temperatures on post-irradiation growth of penicillium exposed to ultraviolet

The growth of Penicillium notatum colonies after UV irradiation of dried mycelium or spores was studied in relation to post-irradiation temperature and salt environment. Dried mycelium and spores behaved differently with respect to sensitivity to temperature, salts and UV, especially the latter. Threshold inhibitory doses for spores were modified markedly either at 4°C or in magnesium and calcium chlorides. It is suggested that these temperature and salt effects are related to prevention of photochemical membrane damage.     

Geotropic sensitivity exhibited by single hornets: The influence of caste, age, light and temperature

Hornet ( , Hymenoptera: Vespinae) workers, queens and males, aged 0–24 hours (i.e. juveniles) and 24 hours and more (i.e. adults) were tested for their responses to changes in the direction of the gravitational force while placed on a flat surface gradually tilted between 0.5° and 180°. The tests were run on non-blind and blind hornets, at temperatures ranging between 18°C and 35°C, in daylight as well as in the dark. Up to 18 hours of age, negative phototaxis prevailed among the hornets, which displayed a clear preference for remaining in the dark regardless of the geotropic position. Between 18–24 hours of age, there was gradual appearance of a sensitivity to change in the geotropic position. Above 24 hr of age, the hornets became sensitive to changes in their declinations, with workers becoming sensitive at a 3–5° declination, queens at 4–5° and males at a declination of 8–19° from the horizontal. Hornet response takes the form of an upward climb, to the highest point of the test surface. Such response required a temperature exceeding 24.8–25°C for workers, 23.2°C for queens and 20.8–21°C for males.     

Geminga pulsar observations with gamma-telescope Gamma-1

The Geminga light curve obtained with the “Gamma-1” telescope features two peaks separated by 0.5 ± 0.03 period. The light curve is pronounced for γ-quanta energies higher than 400 MeV. The pulsed flux upper limit (1σ) in the energy interval 50 – 300 MeV is 6·10⁻⁷ cm⁻²sec⁻¹. For energies >300 MeV the pulsed component power law spectrum has an exponent 1.1 _−0.3^+1.1 and an integral flux (1.1±0.3)·10⁻⁶ cm⁻²sec⁻¹.     

Interplanetary scintillations of PKS 2314+03 during occultation by comet Halley

Strong interplanetary scintillations (IPS) of the quasar 2314+03 were recorded at 103 MHz at Thaltej-Ahmedabad, India with a transit type correlation interferometer on 18, 19 and 20 December 1985, as the radio source was predicted to be occulted by the ion tail of the comet Halley.

On 18th through 20th very strong scintillations, with periodicities of 1 sec average were observed, their amplitude progressively decreasing as the source approached the tail-end. The rms variations of scintillating flux of the source on 18, 19 & 20 were about 18, 11 & 4.7 Jy, as against 3.3 Jy on control days 17 and 21 December for solar elongation of 87°.

Assuming Gaussian irregularities with weak scattering, the rms density variations, ΔN, of 10, 6, 3 and 1 elec./cm³ on 18 through 21 December, from the comet nucleus towards its tail-end, varied as (ΔN) ∝ r^−3.3 as against (ΔN) ∝ r⁻² in the solar plasma.

Quasi-periodic modulations of the enhanced scintillating flux possibly imply 10⁴ km scale-size ion condensations and mean electron density of 10³ to 10⁴ electrons/cm³ in the Halley's plasma tail.     

Mesospheric nitric oxide and ozone measurements in polar winter at 69°N

Two rocket experiments were carried out just before and after the polar night at Andoya (69°N), Norway to investigate transport of nitric oxide produced by auroral processes into the middle atmosphere and its influence on the ozone chemistry. Nitric oxide densities of (2–5) × 10⁸cm⁻³ found in the 70–90 km region are one to two orders of magnitude larger than those at middle latitudes. The influence on ozone densities in the 70–90 km region due to such enhanced nitric oxide abundance is found to be insignificant as compared to that due to transport in the middle of February. The larger ozone densities found in February (in spite of longer sunlit duration) than in November in the 40–60 km region again support predominance of transport over photochemical loss.     

Calcium abundance measurements using the Yohkoh BCS

Soft X-ray observations by SMM and other spacecraft have shown that the abundance of certain elements in solar corona varies from flare to flare. In this study, observations made by the Yohkoh Bragg Crystal Spectrometer (BCS) in helium-like Ca XIX have been analysed, and Ca abundance determined for 177 flares observed during the first four years of the mission (1991–1995). The average abundance of Ca relative to H for all flares is A_Ca = (3.64±0.39) × 10⁻⁶. As with an earlier study of SMM data, the abundance is found to be enhanced compared to the photosphere ((2.24±0.10) × 10⁻⁶), and with only minor variation from flare to flare. However, the absolute value and range of values determined by this study is smaller than in the previous study; these differences are discussed.     

Mercury: can any ice exist at subpolar regions?

The heat transfer in a regolith subsurface layer of thickness 20 m has been theoretically simulated for the areas near Mercury's north pole aiming at the clarification of the possible existence of subsurface ice formations of different form. The paper considers different models of the icy regolith structure and composition: pure uniform amorphous ice; a porous dispersive system with ice-filled pores and voids; permafrost. For comparison the heat transfer in dry iceless regolith has been considered as well. It has been shown that the line of maximum distribution of subsurface icy formations depends on the icy regolith model, but for any one in the “hot” regions it does not go below 70°. For the “cool” regions this line has been shown to go from 5° to 10° southward than that for the “hot” ones. The possible thickness of icy regolith near the pole has been estimated for different models assuming an interior heat flow of 15 mW m⁻². It has been shown that the maximum thickness of this layer takes place at the pole and is equal to 10 km for any model.     

High spectral resolution Fabry-Perot interferometer measurements of comet Halley at H-alpha and 6300 Å

A 40.6 cm Newtonian telescope has been interfaced to the Fabry-Perot interferometer at the Arecibo Observatory to make high spectral resolution measurements of Comet Halley emissions at 6562.72 Å (H-alpha) and 6300.3 Å (OI). In March 1986 the H-alpha surface brightness for a 5′.9 field of view centered on the comet nucleus decreased from 39±7.8 rayleighs on 12 March to 16±3.8 rayleighs on 23 March. The atomic hydrogen production rate on 12 March 1986 was 1.62±0.5 × 10³⁰ s⁻¹, and on 23 March 1986 it was 6.76±2.3 × 10²⁹ s⁻¹. Using spectral resolution of 0.196 Å, we found the atomic hydrogen outflow velocity to be approximately 7.9±1.0 km s⁻¹. In general, the H-alpha spectra are highly structured, and indicative of a multiple component atomic hydrogen velocity distribution. An isotropic outflow of atomic hydrogen at various velocities is not adequate to explain the spectra measured at H-alpha. The 6300.3 Å emission of O(1D) had a surface brightness of 81±16 rayleighs on 15 March 1986, and 95±11 rayleighs on 17 March 1986. After adjustment for atmospheric extinction, the implied O(1D) production rate on 15 March is 6.44±3.0 × 10²⁸ s⁻¹, and the production rate on 17 March is 5.66±2.7 × 10²⁸ s⁻¹. These spectra included a feature at 6300.8 Å that we attribute to NH₂. The brightness of this emission feature was 37±11 rayleighs on 15 March.     

Marangoni and buoyant convection in an open cavity under reduced and under normal gravity

We give the first full analysis of the microgravity experiment MKB ( arangoni onvektion im offenen oot) conducted on board of the space shuttle during the D1-mission in 1985, together with that of some ground based experiments on thermocapillary flow in a rectangular liquid volume with free upper surface. Streamlines and velocity profiles under μ-g and l-g are presented and discussed. Strong thermocapillary flow was observed under μ-g after the rupture of a dirt-film suppressing surface tension forces. The Nusselt-number under microgravity due to thermocapillary flow for Ma = 4.6 · 10⁵ is at least Nu = 6 ± 1. Under l-g a separation of the surface tension driven convection roll of hot fluid circulating on top of colder fluid was found for large Ma. The coupling and decoupling of buoyant and thermocapillary forces has been further studied in an experiment varying the temperature gradient along the free surface independently from that in the bulk fluid.     

Extreme starburst or central activity in X-ray luminous IRAS galaxies

ROSAT All Sky Survey observations of IRAS galaxies have revealed up to now a number of 10 optically non-Seyfert galaxies with X-ray (0.1–2.4) luminosities up to a few 10⁴³erg · s⁻¹ (Boller et al. 1992). The sources are brighter than previous detection limits of a few 10⁴¹erg · s⁻¹ as found by Stocke et al. (1991) or Green, Anderson and Ward (1992) for Einstein sources. The optical classification is based on follow-up observations which indicate clearly the non-Seyfert (LINER and HII region-like galaxies) nature. Our investigations reveal that galaxies classified as non-Seyferts on the basis of optical spectroscopy may reach exceptionally high X-ray luminosities which are similar to that of Seyfert galaxies. On the basis of the present observational material we suppose a hidden low luminosity AGN in the centre of these objects as the source of energy production. The objects are of interest when evaluating starburst versus central activity.     

Near infrared observations of comet Halley from Vega 2

Spatial distribution of the continuum radiation in the range of 0.95–1.9 μm presumes total dust production rate of the comet of 10ρ tonne s⁻¹ (ρ is the dust material density) and its angular distribution proportional cos . Observations of the water vapor band at 1.38 μ m reveal strong jets, their time shift from the dust jet measured in situ is consistent with gas velocity of 0.82±0.1 km s⁻¹ and dust velocity of 0.55±0.08 km s⁻¹. The OH vibrational-rotational bands observed are excided directly via photolysis of water vapor. Water vapor production rate deduced from the H₂O band and OH band intensities is 8×10²⁹ s⁻¹. Intensity of the CN(0,0) band result in the CN column density of 9×10¹² cm⁻², i.e. larger by a factor of 3 than given by the violet band.     

X-ray measurements of the dark matter distribution in clusters of galaxies with Chandra

We investigate the dark matter distributions in the central region of two clusters of galaxies (A1835 and MKW3S) using Chandra data. N-body simulations in the standard cold dark matter (CDM) model predict the dark matter distribution shows a cuspy dark matter profile: ρ(r) ∝ r, with in the range 1–2, while observations of dwarf and low surface brightness galaxies seem to favor the presence of a relatively flat core: 0 <  < 1. To investigate the dark matter distributions in the central region of clusters of galaxies, we analyze the Chandra data of A1835 and MKW3S with a deprojection method. We derive the mass profiles without the assumption of analytical models. We examine the inner slope of derived mass profiles assuming the dark matter profile is described with a power-law expression. The values of the slope are 0.95 ± 0.10 for A1835 and 1.33 ± 0.12 for MKW3S within the radius of 200 kpc. These are consistent with the result of the CDM simulations. However, within the radius of 100 kpc, the value of is less than unity for A1835 (0.47 ± 0.31). Our result implies that the central dark matter profile of some clusters cannot be described by CDM halos.     

Large light X-ray optics: basic ideas and concepts

One of the main guidelines for future X-ray astronomy projects like, e.g., XEUS (ESA) and Generation-X (NASA) is to utilize grazing-incidence focusing optics with extremely large telescopes (several tens of m² at 1 keV), with a dramatic increase in collecting area of about two order of magnitude compared to the current X-ray telescopes. In order to avoid the problem of the source's confusion limit at low fluxes, the angular resolution required for these optics should be superb (a few arcsec at most). The enormous mirror dimensions together with the high imaging performances give rise to a number of manufacturing problems. It is basically impossible to realize so large mirrors from closed Wolter I shells which benefit from high mechanical stiffness. Instead the mirrors need to be formed as rectangular segments and a series of them will be assembled in a petal. Taking into account the realistic load capabilities of space launchers, to be able to put in orbit so large mirror modules the mass/geometric-area ratio of the optics should be very small. Finally, with a so large optics mass it would be very difficult to provide the electric power for an optics thermal active control, able to maintain the mirrors at the usual temperature of 20 °C. Therefore, very likely, the optics will instead operate in extreme thermal conditions, with the mirror temperature oscillating between −30 and −40 °C, that tends to exclude the epoxy replication approach (the mismatch between the CTE of the substrate and that of the resin would cause prohibitively large deformations of the mirror surface profiles). From these considerations light weight materials with high thermal–mechanical properties such as glass or ceramics become attractive to realize the mirrors of future Xray telescopes. In this paper, we will discuss a segments manufacturing method based on Borofloat^TM glass. A series of finite element analysis concerning different aspects of the production, testing and integration of the optics are also presented as well.     

A comprehensive study on middle atmospheric thermal structure over a tropic and sub-tropic stations

The general characteristics of middle atmospheric thermal structure have been studied by making use of the Rayleigh lidar data collected over the period of about four years (1998–2001). Here, the data has been used from two different stations in the Indian sub-continent in tropics (Gadanki; 13.5°N, 79.2°E) and in sub-tropics (Mt. Abu; 24.5°N, 72.7°E). The observed monthly mean temperature profiles are compared with different model atmospheres (CIRA-86 and MSISE-90). We observed, the mean temperature profiles have closer agreement with MSISE-90 than CIRA-86. The temperature profiles measured by lidar and HALOE satellite overpass nearby lidar site are generally in agreement with each other. The systematic and statistical errors in deriving temperature are found to be uniform for both the stations, as 1 K at 50 km, 3 K at 60 km and 10 K at 70 km. The special features of mesospheric temperature inversion (MTI) and double stratopause structure (DBS) are also addressed for both the stations.