Functional state of chromatin and proliferative activity of meristematic cells in pea seedlings under various clinostatic conditions.

Data from a complex cytochemical analysis show that the functional state of chromatin and the level of the cell proliferative activity may be reliable cytological criteria for primary structural and functional changes that result in disturbances of plant growth and development. Autoradiographic and cytophotonetric studies made it possible to establish certain differences, induced by fast rotation (50 rev/min) on the clinostat, in the chromatin state and cell reproduction of the pea seedling root meristem for the initial stages of plant development. There were no essential differences for the given parameters under slow (2 rev/min) clinostatic conditions.     

Effects of altered gravity on plant cell processes: results of recent space and clinostatic experiments.

Space and clinostatic experiments revealed that plant cell structure and metabolism rearrangements depend on taxonomical position and physiological state of objects, growth phase and real or simulated microgravity influence duration. It was shown that clinostat conditions reproduce only a part of microgravity biological effects. It is established that various responses occur in microgravity: 1) rearrangements of cytoplasmic organelles ultrastructure and calcium balance; 2) physical-chemical properties of the plasmalemma are changed; 3) enzymes activity is often enhanced. These events provoke the acceleration of growth and differentiation of cells and their aging as a result; at the same time some responses can be considered as cell adaptation to microgravity.     

Biological effects of weightlessness and clinostatic conditions registered in cells of root meristem and cap of higher plants.

Research in cellular reproduction, differentiation and vital activity, i.e. processes underlying the development and functioning of organisms, plants included, is essential for solving fundamental and applied problems of space biology. Detailed anatomical analysis of roots of higher plants grown on board the Salyut 6 orbital research station show that under conditions of weightlessness for defined duration mitosis, cytokinesis and tissue differentiation in plant vegetative organs occur essentially normally. At the same time, certain rearrangements in the structural organization of cellular organelles--mainly the plastid apparatus, mitochondria, Golgi apparatus and nucleus--are established in the root meristem and cap of the experimental plants. This is evidence for considerable changes in cellular metabolism. The structural changes in the subcellular level arising under spaceflight conditions are partially absent in clinostat experiments designed to simulate weightlessness. Various clinostatic conditions have different influences on the cell structural and functional organization than does space flight. It is suggested that alterations of cellular metabolism under weightlessness and clinostatic conditions occur within existing genetic programs.     

Growth and development in higher plants under simulated microgravity conditions on a 3-dimensional clinostat.

Growth and development of etiolated pea (Pisum sativum L. cv. Alaska) and maize (Zea mays L. cv. Golden Cross Bantam) seedlings grown under simulated microgravity conditions were intensively studied using a 3-dimensional clinostat as a simulator of weightlessness. Epicotyls of etiolated pea seedlings grown on the clinostat were the most oriented toward the direction far from cotyledons. Mesocotyls of etiolated maize seedlings grew at random and coleoptiles curved slightly during clinostat rotation. Clinostat rotation promoted the emergence of the 3rd internodes in etiolated pea seedlings, while it significantly inhibited the growth of the 1st internodes. In maize seedlings, the growth of coleoptiles was little affected by clinostat rotation, but that of mesocotyls was suppressed, and therefore, the emergence of the leaf out of coleoptile was promoted. Clinostat rotation reduced the osmotic concentration in the 1st internodes of pea seedlings, although it has little effect on the 2nd and the 3rd internodes. Clinostat rotation also reduced the osmotic concentrations in both coleoptiles and mesocotyls of maize seedlings. Cell-wall extensibilities of the 1st and the 3rd internodes of pea seedlings grown on the clinostat were significantly lower and higher as compared with those on 1 g conditions, respectively. Cell-wall extensibility of mesocotyls in seedlings grown on the clinostat also decreased. Changes in cell wall properties seem to be well correlated to the growth of each organ in pea and maize seedlings. These results suggest that the growth and development of plants is controlled under gravity on earth, and that the growth responses of higher plants to microgravity conditions are regulated by both cell-wall mechanical properties and osmotic properties of stem cells.     

Optical and electron-microscopic studies of the Funaria hygrometrica protonema after cultivation for 96 days in space

$\text{Funaria hygrometrica}$ protonema cells grown in the “IFS-2” (Inoculating fixing system) for 96 days on board the Salyut 6 — Soyuz 32 orbital scientific station were examined by light and electron-microscopy. Investigation of experimental and control cells of the moss protonema showed common features as well as distinctions in their structure. Protonema cells of $\text{Funaria hygrometrica}$ both differentiate and undergo photosynthesis during space flight. Changes in cell shape, decreased cell size, a reduction in the volume of starch granules, and altered chloroplast structure were observed.     

On different sensitivities of microorganisms to lowered gravitation

The influence of lowered gravitation on biomass and CO₂ production in $\text{B.megaterium}$, a xerophyte, and $\text{Spirillum azotocolligens}$, an aqueous spirillum, in liquid nutrient medium on a horizontal clinostat at 0.1 $\text{g}$ has been studied. As controls we considered: 1) growth under stationary conditions of cultivation with test tubes oriented horizontally; 2) growth on a synchronously revolving centrifuge; and 3) growth on a swing with stirring. A horizontal clinostat at 0.1 g stimulates biomass production and CO₂ release in $\text{B.megaterium}$ as compared with the controls. $\text{Spirillum azotocolligens}$ growth is reduced as a result of clinostating. The best development and CO₂ production are observed under stationary conditions. The results do not support the assumption that microorganisms living in water are more resistant to lowered gravitation than those living in soil.     

The role of weightlessness in the genetic damage from preflight gamma-irradiation of organisms in experiments aboard the Salyut 6 orbital station

The effect of weightlessness on chromosomal aberration frequency in preflight irradiated $\text{Crepis capillaris}$ seeds, on the viability, fertility and mutation frequency in $\text{Arabidopsis thaliana}$, and on the frequency of nondisjunction and loss of X chromosomes in preflight irradiated $\text{Drosophila melanogaster}$ gametes was studied aboard the Salyut 6 orbital station. The following effects were observed: a flight-time dependent amplification of the effects of preflight

-irradiation in $\text{A}$. $\text{thaliana}$ with respect to all the parameters studied; unequal effects in seeds and seedlings of $\text{Crepis capillaris}$; and a significant increase in the frequency of nondisjunction and loss of chromosomes during meiosis in $\text{Drosophila}$ females. These observations are discussed in terms of the data of ground-based model experiments and flight experiments with a different time of exposure of objects to weightlessness. An attempt is made to elucidate the role of weightlessness in the modification of ionizing radiation effects.     

Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Magnetic flux ropes in the Venus ionosphere: In situ observations of force-free structures?

Force-free magnetic structures with cylindrical geometry appear under a variety of conditions in nature. Filamentary helical magnetic structures are observed to be associated with prominences and flares in the solar atmosphere, and can arise in superconductors and laboratory plasmas. Another example of cylindrical quasi-force-free configurations appears to exist in the Venus ionosphere. Magnetic flux ropes with diameters of ～20 – 30 km have been observed by the Pioneer Venus Orbiter to be a nearly ubiquitous feature of the dayside Venus ionosphere. Models of flux ropes suggest that many of these structures tend to be quasi-force-free, i.e., $\text{J}$×$\text{B}$～0, while others are correlated with pressure variations in the ambient thermal plasma, $\text{J}$×$\text{B}$=-?(nkT).     

Biological investigations aboard the biosatellite Cosmos-1129

Experiments on insects, higher plants and lower fungi were carried out aboard the biological satellite Cosmos-1129, in Earth orbit, from 25 September to 14 October 1979. The main objective of these experiments was to gain more profound knowledge of the effect of weightlessness on living organisms and to study the mechanisms by which these various organisms with different life cycles can adjust and develop in weightlessness. Experiments on insects ($\text{Drosophila melanogaster}$) were made with a view towards understanding gravitational preference in flies, the life cycle of which took place on board the biosatellite under conditions of artificial gravity. Experiments on higher plants ($\text{Zea mays, Arabidopsis taliana, Lycopersicum esculentum}$) and lower fungi ($\text{Physarum polycephalum}$) were performed.     

The action of simulated and true weightlessness on the digestive tract of rats

Rats on board the Soviet Cosmos 936 satellite for 18$\text{1}\text{2}$ days showed a decreased glycoprotein secretion from the salivary mucous glands, stomach and intestine, and an increased leucine aminopeptidase and acid phosphatase content from the small intestine. Grimelius positive cells were activated. One group of rats were centrifuged at 1 g during the flight to simulate terrestrial gravity. Some investigations have suggested that under these conditions muscular and cardiac disorders diminished. In the digestive tract the benefits of centrifugation at 1 g are minimal and limited to a few glycoprotein components. The digestive changes are probably the expression of a stress response, unrelated to weightlessness. Similar changes, concomitant with a glycocorticoid hypersecretion, were found in rats after 15 days of hypokinesia on Earth. These digestive changes persisted even in adrenalectomized rats.     

Ralicon anodes fabricated by electron beam lithography for image photon counting detectors

The Anger wedge and strip anode event location system developed for microchannel plate image photon detectors at the Space Sciences Laboratory of the University of California, Berkeley, has been extended in the present work by the use of electron beam lithography (EBL). Computer-aided design methods have been used to develop several types of RALICON ($\text{R}$eadout $\text{A}$nodes of $\text{Lit}$hographic $\text{Con}$struction) for use in photon counting microchannel plate imaging detectors. These anodes are suitable for linear, two dimensional or radial position measurements and they incorporate novel design features made possible by the EBL fabrication technique which significantly extend their application relative to published wedge-strip anode designs.     

Survival of microorganisms in space: A review

Spores of $\text{Bacillus subtilis}$ were exposed to selected factors of space (vacuum, solar UV radiation, heavy ions of cosmic radiation), and their response was studied after recovery. These investigations were supplemented by ground-based studies under simulated space conditions. The vacuum of space did not inactivate the spores. However, vacuum-induced structural changes in the DNA, and probably in the proteins, caused a supersensitivity to solar UV radiation. This phenomenon is caused by the production of specific photoproducts in DNA and protein, which cannot be removed by normal cellular repair processes. In vegetative bacterial cells, exposed to vacuum, cell dehydration led to damage of the cell membrane, which could be partly repaired during subsequent incubation. The high local effectiveness of the cosmic heavy ions further decreases the chance that spores can survive for any length of time in space. Nonetheless, a spore travelling through space and protected from ultraviolet radiation could possibly survive an interplanetary journey. Such a situation favors panspermia as a possible explanation for the origin of life.     

Ultraviolet spectroscopy of cometary comae: An update

Since its launch in 1978 the $\text{International Ultraviolet Explorer}$ ($\text{IUE}$) satellite observatory has been used to record ultraviolet spectra of nearly two dozen comets. These observations have been applied principally to studies of the composition, chemistry and evolution of the gaseous coma and more recently, with the substantially increased data base, to comparative analyses. The observations of Comets Bowell (1982 I) and Cernis (1983?) at a heliocentric distance of ≈ 3.4 AU show these two comets to be virtually identical and pose problems for water ice vaporization models. The most significant recent result from $\text{IUE}$ was the discovery of S₂ in the Earth-approaching comet IRAS-Araki-Alcock (1983d) and the use of the S₂ emission as a monitor of short-term variations in cometary activity. In early 1984, periodic comet Encke was observed for the second time by $\text{IUE}$, this time post-perihelion.     

Global telecommunications needs for the long duration balloon environment

The $\text{Long Duration}$ (LD) balloon environment significantly complicates the means of providing effective telecommunications support when compared to support possible within the conventional $\text{zero-pressure}$ (ZP) environment. This paper will discuss general aspects of supporting two-way telecommunications between ground based facilities and multiple LD balloon payloads.A summary of the LD environment (general operational and NSBF support) is presented as a basis for discussing generic network characteristics. General LD telecommunication needs are highlighted and a preliminary systems model of an “ideal” LD telecommunication network is introduced.     

What is climate?

The present meaning of the word $\text{climate}$ evolved simultaneously with the formulation of research priorities which are regarded as necessary to explore the causes and, consequences of observed environmental changes. Climate can be understood in its widest sense as the totallity of influences to which the biosphere is exposed. Of primary interest are changes of these environmental conditions which are caused by the variability of the $\text{climate system}$. This expression is used in the sense of a physical system in which energy conversions take place, and which is forced, e.g. by the solar input, geologic events and man's activity. The climate system is composed of five major subsystems: atmosphere, hydrosphere, land surfaces, cryosphere and biosphere. These subsystems interact in a complex non-linear manner in different time scales. Major climate determining processes are outlined and the research priorities are discussed, which are regarded essential to investigate them and their interactions.     

Immunolocalization of an annexin-like protein in corn.

Although calcium has been proposed to be an important regulatory element in plant gravitropic growth, as yet no specific function of Ca2+ in growth regulation has been discovered. Our recent studies on a Ca(2+)-binding protein in pea seedlings called p35 indicate that it is a member of the annexin family of proteins and may play a key role in growth regulation through its function in delivering polysaccharides needed for wall construction. We previously reported the isolation of p35 from pea plumules and the production of polyclonal antibodies to it. Immunolocalizaton analyses of p35 in pea tissues revealed high levels of staining in secretory cell types such as developing vascular cells and outer root cap cells. To test how general was the occurrence and distribution of this annexin-like protein in plant cells we initiated an analysis of annexins in the monocot corn using immunological techniques. Our results indicate the immunochemical properties and localization of corn annexins are very similar to those reported for pea. They are consistent with the postulate that annexins may play a general role in the regulation of the secretion of wall polysaccharides needed for growth, and thus could be an important target of calcium action during gravitropic growth.     

Ultrastructural analysis of organization of roots obtained from cell cultures at clinostating and under microgravity.

Data are presented of a comparative analysis on rhizogenesis in the Arabidopsis thaliana tissue culture growing in a solid nutrient medium under stationary conditions, clinostatic conditions and microgravity. Tissue samples weighing 100 mg. were set in the Petri dishes and placed in a horizontal slow clinostat /2 revs/min/. After 14 days of growth they were analyzed. On clinostating the number of roots formed from the callus cells was approximately one half the control. The formed root cap manifested no essential differences, in comparison with the stationary control, in the number of layers and cell sizes in its layers. In callusogenic roots, formed from clinostated cells, differentiation including root cap cells, proceeds without noticeable deviations from the norm. At the same time, gravireceptor cells do not function under these conditions. This is clearly displayed at a structural level in the location of amyloplasts-statoliths throughout the cytoplasm. The callus cell cultures experienced microgravity for 8 days. The number of formed roots under the influence of this factor was 36% relative to the stationary control. Root cap formation was abnormal. Gravireceptor cells did not formed under microgravity.     

Calcium gradient in plant cells with polarized growth in simulated microgravity.

Plant cells characterized by apical growth, for example, root hairs and apical cells of moss protonema, are a convenient model to address the problem of gravity response mechanisms including initiation of cell polarity. The fluorescent calcium probe, chlorotetracycline, allowed us to display the calcium distribution gradient in these cells. Irradiation by red light led to a sharp decrease in the Ca2+ ion activity in cells. During clinostatting in darkness the pattern of calcium influx and distribution changes inconsiderably as compared with control; in root hairs calcium is detected mainly in their apices and bases as in control. Addition of chlorpromazine to the medium probably increases the influx and accumulation of Ca2+ ions. Under data obtained confirm speculations on the Ca2+ ion functional role for the apical growth of plant cells and may suggest the participation of gravity in redistribution or activation of ion channels, calcium channels included, in the plasmalemma.     

Effects of clinorotation on the polysaccharide content of resynthesized walls of protoplasts.

Changes in cellulose and callose content during cell wall regeneration in Brassica oleracea protoplasts have been examined by cytofluorimetry following their exposure to the conditions of the horizontal clinostat (2 r.p.m.) for 10 days. In comparison with controls, cellulose content decreased 4-fold and 28% of the protoplasts failed to resynthesize a wall in the clinorotated sample. The callose content was almost doubled in clinostated cells. Callose synthesis fluctuated in both control and clinorotated protoplasts. The results support the idea that inhibition of cellulose synthesis in protoplasts grown on the clinostat is caused by a change of plasmalemma fluidity and functioning, and also by a disturbance to the state of cytoplasmic calcium under conditions of simulated microgravity.